Erwinia carotovora

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A Microbial Biorealm page on the genus Erwinia carotovora

Contents

Classification

Higher order taxa

Kingdom: Bacteria Phylum: Proteobacteria Class: Gammaproteobacteria Order: Enterobacteriales Family: Enterobacteriaceae Genus: Erwinia Species: carotovora (7)

Species

Erwinia amylovora; Erwinia aphidicola; Erwinia billingiae; Erwinia carotovora; Erwinia chrysantum; Erwinia mallotivora; Erwinia papayae; Erwinia persicina; Erwinia psidii; Erwinia pyrifoliae; Erwinia rhapontici; Erwinia toletana; Erwinia tracheiphila (6) Subspecies: Erwinia carotovora subsp. atroseptica, Erwinia carotovora subsp. betavasculorum, Erwinia carotovora subsp. carotovora, Erwinia carotovora subsp. odorifera, Erwinia carotovora subsp. wasabiae (6)

Description and significance

Erwinia carotovora is a rod shaped bacterium that was named after the crop of carrots from which it was first isolated. The bacterium infects a variety of vegetables and plants including carrots, potatoes, cucumbers, onions, tomatoes, lettuce and ornamental plants like iris.(2) These widespread microbes can be found in soil, guts of insects, water and suspended aerosols in air.(1) A major problem in agriculture, the microbes ceaselessly invade crops of potatoes and other vegetables in the fields or in storage that cause plant tissues to become soft and watery which eventually turn slimy and foul-smelling.(2) Whereas Erwinia carotovora subsp. atrosepticum’s pathogencity is restricted to potatoes in temperate temperatures, Erwinia carotovora subsp. carotovora infects a much broader host of plants, including potatoes, in warmer climates.(3)

Genome structure

Only the genome of Erwinia carotovora subsp. atroseptica strain SCRI1043 (Eca 1043) has been sequenced. The sequencing shows that the genome of Erwinia carotovora subsp. atroseptica is a single 5,064,019 base pair circular chromosome with 50.97 percent of guanine and cytosine, approximately 4,491 coding sequences (CDSs), seven rRNA operons, 76 tRNAs, and around 25 stable noncoding RNAs. It has acquired eleven horizontally acquired genomic islands (HAIs) that are predicted to allow nitrogen fixation, phenazine antibiotic production, phytotoxin synthesis and the production of adhesion proteins used for host attachment. In addition, the microbe acquired genes for opine catabolism. The bacterium contains all six Gram-negative protein secretion systems. Type one and type two secretion systems produce plant cell-wall degrading enzymes (PCWDEs) and other pathogenic determinants. There are twenty pectinase genes and seven CDCs that contribute to the production of PCWDEs. The type III secretion systems secrete proteins into eukaryotic cells where they contribute to pathogenesis by disrupting host cell signal transduction and cellular processes.(9) A mutation of the genes that resemble the type four secretion system locus of Agrobacterium tumefaciens negatively affects pathogenicity. The type five secretion system produces an auto transporter and the two-partner secretion systems may contribute to host cell attachment. (3)

Cell structure and metabolism

Erwinia carotovora is a Gram-negative, rod-shaped bacterium that lives alone or aggregates into pairs and chains. Non-spore forming and peritrichously flagellated, it is a facultative anaerobe that is catalase negative and oxidase positive. Erwinia carotovora produces a number of extracellular plant cell wall degrading enzymes such as pectic enzymes that degrade pectin, cellulase that degrades cellulose, hemicellulases, arabanases, cyanoses and a protease. As a mesophilic bacterium, Erwinia carotovora thrives the most in the temperature range between 27 and 30 degrees Celsius.(1) The genome sequencing of Erwinia carotovora subsp. atroseptica indicates that this microbe may not only be capable of nitrogen fixation and opine catabolism, but may also utilize around eighty ATP binding cassette transport systems.(3)

Ecology

In addition to infected plants, Erwinia carotovora can also be found in the guts of insects and bodies of water introduced by aerosols, runoff into rivers and dumping of potatoes. After rainfall upon diseased plants, an aerosol containing the bacteria is created. Fifty percent of the bacteria that become suspended in aerosols can survive for five to ten minutes and may travel for miles.(1)

Pathology

Erwinia carotovora is a plant pathogen that causes cell death through plant cell wall destruction by creating an osmotically fragile cell. This is achieved through the production of PCWDEs (3) such as extracellular pectic enzymes and cellulase that break down pectin and cellulose, respectively.(1) Either by plant to plant transfer or insect to plant transfer, this organism causes soft-rot diseases of many plants and vegetables that eventually become characterized as slimy and foul smelling.(1,2) Erwinia Carotovora subsp. atroseptica strictly infects potatoes that may also produce a nonribosomal peptide phytotoxin that induces necrosis by electrolyte leakage through transmembrane pore formations. In addition, Eca1043 is predicted to synthesize large hemagglutinin-like proteins, pili and fimbrial proteins for host adhesion. Finally the possible horizontal genetic transfer of genes that resembled type four secretion systems of Agrobacterium tumefaciens may contribute to pathogencity because the mutation of these genes negatively affected virulence.(3)

Application to Biotechnology

So far, none has been found.

Current Research

In the article "Using aqueous chlorine dioxide to prevent contamination of tomatoes with Salmonella enterica and Erwinia carotovora during fruit washing", it was found that washes with chlorine dioxide can be used as a disinfectant to effectively reduce the number of E. carotovora on fruit surfaces.(4) In the article Erwinia carotovora Evf antagonizes the elimination of bacteria in the gut of Drosophila larvae", it was found that the Erwinia Virulence Factor found in E. carotovorais not a toxin but allows for the accumulation of the bacteria in the anterior midgut of Drosophila larvae.(5) In the recent article "Genome sequence of the enterobacterial phytopathogen Erwinia carotovora subsp. atroseptica and characterization of virulence factors", it was discovered that organism responsible for blackleg and soft-rot disease in potatoes, Erwinia carotovora, demonstrates unexpected metabolic traits such as nitrogen fixation and opine catabolism.(3)

References

1. Perombelon, Michael CM. The Prokaryotes. Second Edition. p2899- - 2921.

2. Wood, M. 1998. Ubi7-new tool for potato breeders. Agricultural Research/January 1998, pp. 12-13.

3. Bell KS, Sebaihia M, Pritchard L, Holden MT, Hyman LJ, Holeva MC, Thomson NR, Bentley SD, Churcher LJ, Mungall K, Atkin R, Bason N, Brooks K, Chillingworth T, Clark K, Doggett J, Fraser A, Hance Z, Hauser H, Jagels K, Moule S, Norbertczak H, Ormond D, Price C, Quail MA, Sanders M, Walker D, Whitehead S, Salmond GP, Birch PR, Parkhill J, Toth IK. "Genome sequence of the enterobacterial phytopathogen Erwinia carotovora subsp. atroseptica and characterization of virulence factors" Proceedings of the National Academy of Sciences of the United States of America 2004 Jul 27;101(30):11105-10. Epub 2004 Jul 19

4. Pao S., Kelsey DF, Khalid MF, Ettinger MR. “Using aqueous chlorine dioxide to prevent contamination of tomatoes with Salmonella enterica and Erwinia carotovora during fruit washing.” Journal of food protection, 2007 Mar;70(3):629-34.

5. Acosta Muniz C, Jaillard D, Lemaitre B, Boccard F. “Erwinia carotovora Evf antagonizes the elimination of bacteria in the gut of Drosophila larvae.” Cellular microbiology, 2007 Jan;9(1):106-19. Epub 2006 Jul 31.

6. List of Prokaryotic names with Standing in Nomenclature. May 03, 2007 http://www.bacterio.cict.fr/e/erwinia.html

7. National Center for Biotechnology Information. April 17, 2007 http://www.ncbi.nlm.nih.gov/sites/entrez?Db=genome&Cmd=ShowDetailView&TermToSearch=412

8. National Center for Biotechnology Information. April 17, 2007 http://www.ncbi.nlm.nih.gov/sites/entrez?db=genomeprj&cmd=Retrieve&dopt=Overview&list_uids=350

9. Hueck CJ. “Type III protein secretion systems in bacterial pathogens of animals and plants.” Microbiology and molecular biology reviews, 1998 Jun;62(2):379-433

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