From MicrobeWiki, the student-edited microbiology resource
A Microbial Biorealm page on the genus Methanofollis
Also see Methanogens overview.
Higher order taxa
Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales; Methanomicrobiaceae
Methanofollis aquaemaris, M. formosanus, M. liminatans, M. tationis
NCBI: Taxonomy Genome
Description and significance
Methanofollis ("a methane-producing bag") is a non-motile, Gram-negative, obligately anaerobic, mesophilic archaeon that produces methane. It grows between the temperatures 20-45°C (optimuim = 34-40°C), and at the pH value of around 7.
The genome of the archaeon has not yet sequenced. The G + C content of the DNA is determined to be 60.0%.
Cell structure and metabolism
The cells of Methanofollis are highly irregular cocci, with diameter of 1.25-2.0µm. The major polar lipids are phospholipids, glycolipids, and phosphoglycolipids.
It utilizes H2/CO2, formate, 2-propanol/CO2, and 2-butanol/CO2 for growth and methanogenesis. No growth has been observed on acetate, trimethylamine, methanol, ethanol, 2-propanol, isobutanol, or 2-butanol as catabolic substrates.
Most species of the archaeon are isolated from anaerobic high-rate wastewater bioreactors or solfataric fields. For example, M. tationis was isolated from a solfataric field on Mount Tatio in the Atacama desert in northern Chile.
Lai, M. C. and Chen, S. C. "Methanofollis aquaemaris sp. nov., a methanogen isolated from an aquaculture fish pond." International Journal of Systematic and Evolutionary Microbiology (2001). Volume 51. p. 1873-1880.
Zellner, G., Boone, D.R., Keswani, J., Whitman, W.B., Woese, C.R., Hagelstein A., Tindall B.J., and Stackebrandt, E. "Reclassification of Methanogenium tationis and Methanogenium liminatans as Methanofollis tationis gen. nov., comb. nov. and Methanofollis liminatans comb. nov. and description of a new strain of Methanofollis liminatans." International Journal of Systematic Bacteriology (1999). Volume 49. p. 247-255.