Small Intestine

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This article is concerned with the microbes residing in one particular niche: the small intestine of the digestive tract. Although most of the human flora (the term used for the bacteria living in the human body) is found in the colon, there are a good number of microbes found in the small intestine. The following article will discuss the different bacteria that contribute to normal functioning and diseased states of the small intestine, with a focus on that of humans.

The Small Intestine (in order from stomach to the large intestine): Duodenum, Jejunum, and Ileum.[1

Contents

Introduction

The human body is not only made up of human cells, but is also comprised of bacterial cells. In fact, microorganisms, or microbes, are so abundant that there are about ten times as many bacteria as there are human cells; while there are 100 trillion human cells, there are 1000 trillion bacteria cells [12]. It is estimated that there are 500 to 100,000 species of bacteria living in the human body [12]. These microbes reside on the skin and mucus surfaces of human tissue, but not within tissues[10].

Some of the bacteria in the body can be beneficial by helping the host obtain and breakdown nutrients, while others can be infectious, causing harm to the host. The microbes living in the host are known as “normal” bacteria and have been dubbed microbiota [11]. However, there are several that indeed benefit the human (a relationship of mutualism), while there are some bacteria that are harmful to the host (a parasitic relationship). Parasitic bacteria can cause many different types of disease, and are thus also considered pathogenic bacteria. As this article is looking at the small intestine, different microbial diseases of the small intestine will be reviewed. It is interesting to note that the growth of pathogenic bacteria can be controlled via means of competitive exclusion provided by the presence of useful bacteria provides [13]; this demonstrates the importance of studying the interaction between different microorganisms in a niche, which will be covered later in this article.

Description of the niche

The small intestine is the site where most of the nutrient absorption occurs such as minerals, sugars, and amino acids. After food has been broken down in the stomach by strong hydrochloric acid, the pyloric sphincter opens and food get pushed into small intestine by peristalsis. There are three major structural parts of the small intestine, duodenum, jejunum, and ileum. Throughout these sections, finger-liked structure called villi increase surface area that helps the absorption of nutrients. The concentration of bacterial activity in the small intestine is around 1 million per milliliter [28]. The microbial processes it undergoes is limited proteolysis and saccharolysis, toxin and SCFA production[25].

The lymphoid appearance of the Peyer's patch under electron microscope [15

Duodenum

The duodenum is a hollowed tube that is situated between the stomach and the jejunum. The duodenum is the shortest section of the small intestine; it is 26cm long, on average. It is mostly responsible for digesting chyme, the food bolus that was created by the churning motion of the stomach. Since it doesn’t have the thick mucus lining like the stomach, the duodenum cannot tolerate the low pH introduced by the chyme. In order to neutralize the pH, the liver secretes bile and pancreas secretes bicarbonate into the duodenum and brings the pH of duodenum up to around 5 and 6 range, a much more tolerable pH for protein and enzymes to function. Enzymes such as lipase, trypsin and chymotrypsin are also secreted into the duodenum to aid digestion. The bacterial density in this section of the small intestine reaches 101 to 103 CFU (Colony Forming Units) /mL and flourishes with gram-positive cocci and rods[2].

Jejunum

The jejunum is 2.5m long, and is the site of absorption. The pH is 7-8 (slightly alkaline). The jejunum is situated right after the duodenum and it is about 10 feet long with pH between 7 and 8 [21]. Goblet cells are most numerous in jejunum, although they exist throughout small intestine. The primary functions of these cells are to secrete mucus. Mucus provides protection against pH, stress, and microorganisms by trapping them [22]. As a result of this, the bacterial density rise to 104-107 CFU/mL and obtains various microbes such as Enterococcus faecalis, lactobacilli, diphtheroids, and the yeast Candida albicans.[25]

Ileum

The ileum is the last section of the small intestine. Like the jejunum, it is also around 10 feet long and its pH lies between 7 and 8. The bacterial density here is also 104-107 CFU/mL and has a microbe community similar to that of the colon. The Ileum is responsible for most of the food and liquid absorption, and the unabsorbed matter and waste products are passed into the large intestine.[21]] One unique feature of the ileum is the dominance of Peyer’s patch, a form of lymphoid tissue. The main function of these Peyer’s patches is to provide leukocytes, part of the immune system, to fight against foreign microorganisms [1].

Image of the Paneth cell under the 1BAS 2 Image Analyzer.[20

Protection against bacteria

Functionally similar to neutrophils, the most abundant form of white blood cell in human, paneth cells on the crypt in the lumen of the small intestine provide host defense against harmful bacteria by primarily secreting antibacterial molecules defensins, or alpha-defensins when exposed to both Gram positive and negative bacteria. Defensins kills the bacteria cells by disrupting their membrane function. Defensins peptides contain hydrophobic and positively-charged regions that can interact with bacteria’s negatively phospholipids membrane by forming pores. This leads to the weakening of the membrane and causing lysis of the bacterial cell. Since most bacteria have higher concentration of negatively charged membrane than normal vertebrae cell membrane, they interact well with defensins’ positively charged regions and sparing the normal vertebrae cells that are needed for normal metabolic functions. Paneth cells also secrete lysozyme and phospholipase A2, which both have antimicrobial functions as well. With the addition of these two agents, paneth cells not only have the ability to kill bacteria, but even fungi and some enveloped viruses as well [14].

Adjacent communities

Stomach

The stomach is located directly above the small intestine, and it is separated from the duodenum by the pyloric sphincter. As mentioned above, the duodenum has a neutral pH, which is needed because inside the stomach, gastric juice is secreted in order to provide the acidic environment needed to convert the inactive pepsinogen to the active pepsin. As the chyme (the semi-digested foodstuff) enters the duodenum, bile is secreted by the liver through the bile duct, to neutralize the acidic pH from the stomach.

Large Intestine

The colon is connected to the other end of the small intestine, at the ileum. It contains an incredibly large number of bacteria, at a concentration of 1011-1012 CFU/mL; the highest concentration found in any ecosystem. The colon contains 400 different types of species which is mostly anaerobic, gram-positive and gram-negative cells. The large intestine absorbs water, minerals, and organic acids. The microbial processes it undergoes is proteolysis, saccharolysis, toxin and SCFA production [2].

Conditions under which the environment changes

The physical conditions of the small intestine can change if there is diarrhea, ulcers, or infections. For example, one may have to take medications like antacid due to conditions such as heart burn or acid reflux into the esophagus. However, one side effect of taking such medication is the decrease of acidity of stomach acid due to neutralization of hydrochloric acid with carbonate from antacid. The result of this is that stomach cannot effectively kill the bacteria that enters the body, which increase the vulnerability of the gastrointestinal tract to bacterial infection [30]. These will be detailed in the diseases section below.

Resident microbes

Fluorescent image of gut bacteria .[28

Which microbes are present?

The intestinal tract of human body has ten times as many microbial organisms than the rest of the body cells in the body [9]. There are up to 100,000 primarily aerobic organisms per milliliter found in the small intestine, and there are at least 500 bacterial species present inside the intestinal tract [9]. The bacterial environment of the jejunum, the middle section of the small intestine located after the duodenum, consists mainly of gram-positive Streptococci and Lactobacilli [6]. Research done on several healthy volunteers showed the presence of Streptococci, Lactobacilli, Staphylococci, and fungi in the intestinal tract [6]. In the ileocaecal valve, Bacteroides and coliform bacteria are the dominant bacteria present there, in addition to anaerobic Lactobacilli [6]. In the ileum, the last section of the small intestine, the bacterial presence is varied, due to the “backwash contamination of the colon” [6].

Functions of flora[2]

Protective

• The natural flora of the small intestine protect the host by taking up space inside the small intestine. Their presence in the small intestine prevents pathogens from obtaining a foothold in the niche.

• Normal bacteria stimulate the growth of the intestinal lining and the immune system of the intestine.

• The natural flora of the small intestine provides competition for nutrients against pathogens, making it more difficult for pathogens to grow.

• The natural flora of the small intestine produces anti-bacterial products to eliminate competition such as pathogens.

Structural

• The natural flora in the small intestine makes up part of the intestinal barrier.

• The natural flora of the small intestine is critical in the natural development of the immune system.

Metabolic

• The natural flora of the small intestine protect the host by metabolizing carcinogens in dietary foods.

• The natural flora of the small intestine provide the host with synthesized vitamins, such as biotin and folate.

• Help produce vitamin K, which is absorbed and used by the host.

• The bacteria are important even for the muscular activity of the small intestine. Without bacteria, there is reduced muscular activity.

Microbial Adventures: Vol. 1

It was a dark and churning night in the stomach of Bob. Lactobacillus lounged around in the folds of the stomach, waiting for the churning to stop. It had been churning for hours with no end in sight. Then, as suddenly as the churning started, it stopped. LB, as he was known among his fellows, stepped out from the folds and surveyed the environment. The harsh acid environment was held back by only his spore coatings. No, he decided. This was not the place to germinate, I wouldn't last long in this environment. He decided to stay inside his spore, ready to spring forth when the moment was right.

Before he could return to the safety of the fold he had been hiding under, the stomach began to undergo peristalsis, the undulating motion of the stomach rippled across the landscape throwing LB to and fro. He was thrown across the Pyloric Sphincter. There he blacked out.

LB suddenly awoke to the rushing river of bile released by the gall bladder. If LB had a mouth, he would have been spitting out the bile, luckily he did not have a mouth. The bile crashed into the partially digested fat around him, causing the fat to emulsify! Around him a pool of bicarbonate began to form, causing the acids from the stomach to be neutralized. I must be in the duodenum, LB rationalized. This place is much more habitable than the stomach, but its a bit too volatile for me!

LB floated along with the gentle flow of food and other spores, some of the spores eventually decided they liked this environment and decided to stay. It wasn't his place to make judgements on other microbe's choices, so he wished them well and moved along.

It wasn't long before LB arrived to find himself at a bustling city! It was the largest collection of microbes LB had ever seen. The city had looked like it had started at a center point and had expanded outward, a colony had once settled here and had eventually grew into the wonder LB saw before him. Nearby LB heard one microbe say to another "Ah, the Jejunum. You will never find a more wretched hive of scum and villainy." The words of this other microbe were very convincing, and LB decided against germinating in this town. He had overheard rumors of a place even larger and more densely packed than even the jejunum, they were whispers of the colon. LB wished to see the sight whose very description was uttered with secrecy and reverence.

LB stayed packed in his spore and continued to move along, hopeful to see the colon. He traveled a long while and eventually landed in the ileum. The ileum was not the metropolis he had heard about. In fact, it was nearly the same size as the jejunum. The conditions here were more pleasant than the duodenum and the jejunum. Doubt gripped LB, he felt the legendary metropolis slip away. LB had given up hope, LB decided that the ileum would be the place to stay to germinate, replicate, and produce lactic acid. The food was plentiful and the neighborhood was friendly.

Little did he know that the metropolis he was looking for was the colon, and it was just a bit further down the tract. Fortunately for LB, this place made him happier than he would ever be if he had indeed traveled to the colon to live there. For if he had traveled to the colon, he would not have wanted to live there, it is an incredibly crowded place, more so than LB's wildest dreams.

Microbial diseases of the small intestine

Pathogenic bacteria in the small intestine are the cause of a variety of diseases. Specific species of bacteria are responsible, but the balance of bacterial numbers is also important. The human host thus has enzymes specialized in regulating the numbers should they get too high or low, but the small intestine is still at risk for disease.

Peptic Ulcers from Helicobacter Pylori[31]]

Helicobactor pylori under the transmission electron microgram. .[26

Peptic ulcers are sores in the lining of the stomach or the duodenum of the small intestine. They have two different causes: infection by the bacteria Helicobacter pylori (H. pylori), or long-term use of NSAIDs (nonsteroidal anti-inflammatory agents) such as aspirin. Contrary to popular belief, stress and spicy food do not cause peptic ulcers; instead, they merely exacerbate the severity of preexisting ulcers. Once an ulcer develops, abdominal discomfort is the most common symptom, and is sometimes accompanied by nausea, weight loss or poor appetite.

The bacteria H. pylori is a spiral-shaped, gram-negative microaerophile proteobacterium that inhabits the stomach and duodenum. Once a person contracts H. pylori, it attacks the stomach and duodenum by targeting the protective mucus coating. Since mucus usually protects the stomach from the acid, acid can now reach the underlying sensitive lining and break it down. H. pylori is specially adapted to the acidic environment of the stomach due to the secretion of protective neutralizing enzymes, specifically urease, and its spiral shape allows for burrowing and embedding into the intestinal lining. Contrary to popular belief, peptic ulcers are actually predominant in the duodenum rather than the stomach. Since the duodenum has a neutral pH environment, H. pylori is not limited to acidic environments; it has merely adapted to survive in the stomach, and instead changes its living conditions by regulating urease [32].

Surprisingly, H. pylori infection usually does not lead to ulcers; while 20 percent of people under 40 years old are infected, only 10 percent of people actually suffer from peptic ulcers at some point in their lives. It is believed that the bacterium is transferred through food or water, but the exact method of spreading is still unknown; as a result, prevention is very difficult, and researchers are currently working on developing a vaccine [29].

The diagnosis of H. pylori-induced ulcers begins with first identifying a stomach ulcer by performing an endoscopy or an upper gastrointestinal series (an X-ray taken after the patient drinks barium to highlight the organs on the film). Next, H. pylori will be diagnosed through a variety of tests: blood, breath, stool and tissue sample. For the blood test, they detect the presence of antibodies to the bacteria. For the breath test, a urea solution containing a special carbon atom is ingested, and if the bacteria is present, the urea breaks down to release the carbon, which is measured. A test on the fecal matter can also be performed, which is also known as the Helicobacter pylori antigen test (HpSA). Finally, the tissue test is saved until the very end because it is the most invasive; a biopsy sample is removed with the endoscope, and either the urease test, a histology test, or a culture test is taken.

Ulcer .[29

Treatment of H. pylori peptic ulcers can take the form of drugs that either target the ulcers or target the bacteria. Those that strictly treat the ulcers both suppress acid secretion and protect the stomach lining, such as histamine receptor-2 (H2) blockers and proton pump inhibitors. Antibiotics are taken to kill the H. pylori. It is not wise to use only one type of medication; to date, it is recommended to undergo a 2-week course of triple therapy, which involves taking two antibiotics to kill the H. pylori, an acid suppressor or a stomach-lining protector. This method has been proven to be effective for over 90 percent of patients suffering from this ailment. The only downside to this treatment is that many pills have to be taken at once. There are also variations to triple therapy: dual therapy (antibiotic, acid suppressor) and quadruple therapy (two antibiotics, an acid suppressor, and a stomach-lining protector).

Tropical Sprue

One of the infectious disorders of the small intestine is tropical sprue. Although there is no precise definition of this disorder as of yet, some scientists define it as “malabsorption of two or more test substances of people living in the tropics" [6]. Symptoms of tropical sprue usually include macrocytic anemia, or abnormally enlarged erythrocytes, due to a malabsorption of folate and vitamin B12 [7]. In early stages of this disease, the ileum and jejunum specifically are affected due to the malabsorption of xylose, glucose, fat, vitamin B12, and folate. After a duration of four months, the mucosa in the small intestine shows partial villous atrophy. Since the villi are the main components of the small intestine that is used for the absorption of nutrients, the absorptive qualities of the small intestine are limited in a patient with tropical sprue, leading to malabsorption of essential nutrients. Though there is no specific known cause of tropical sprue, there is little doubt that it is caused by a severe, acute gastrointestinal infection. No specific microorganism has been identified as the sole cause of tropical sprue, however. Patients with tropical sprue often have a colonization of coliform bacteria in the small intestine. Studies have shown that patients with tropical sprue in areas such as North India, Puerto Rico, Haiti, and in Europeans travelling in India contain coliform bacteria in the jejunum. The bacteria in the intestines of the European travelers included Alcaligenes faecaelis, Enterobacter aerogenes, and the hafnia species [6]. Other patients were infested with Klebsiella pneumonia, Escherichia coli, and Enterobacter cloacae. In one study in Vellore, India, coliform bacteria were present in 29 of 33 patients with tropical sprue [6]. Additional studies in animals have shown that colonization of Enterobacteria in the small intestine causes changes in mucosal structure of the villi. Several experiments have been conducted on rabbit jejunum. Coliform bacteria are usually aerobes or facultative anaerobes, and they contain toxins that increase fluid secretion in the intestines. Another experiment conducted on rabbit jejunum has shown that Klebsiella pneumonia, when entered into the jejunum, decreases xylose absorption while shortening and blunting the villi, inhibiting absorption [6]. Similar results have been shown with a different strain of bacteria, Enterobacter cloacae, is also entered into the jejunum [6].

The standard treatment of tropical sprue is a dosage of tetracycline and folic acid for at least six months [6]. This treatment fixes the mucosal structure of the villi of the small intestine, resolving malabsorption , resulting in patients having increased appetite and weight gain. If there is evidence of vitamin B12 malabsorption in the body during tropical sprue, a vitamin B12 replacement is added as a remedy. Though full recovery is expected, people who stay in the tropical areas where this disease is endemic, have a good chance of falling into a relapse [6]. If this disease is untreated, death eventually occurs, with the victim dying extremely malnourished. However, tropical sprue can be fatal in extremely young or old victims, even with treatment.

Tropical sprue is mostly prevalent in tropical areas, however, not all of these areas have cases of tropical sprue. This may be due to dietary differences of the inhabitants in these regions. There has been a study linking the amount of long-chain unsaturated fatty acids consumed to the occurrence of tropical sprue in the population [6].

Small Intestine Bacterial Overgrowth

Small intestine bacterial overgrowth (SIBO), also known as small bowel bacterial overgrowth (SBBO), is a condition of the small intestine, defined as an increase in the number of bacteria in the upper gastrointestinal tract [9]. It can be caused by any condition that interferes with the muscular activity in the small intestine that can allow bacteria to multiply [8]. SIBO can result in villous atrophy and mucosal inflammation, altering the absorptive functions of the small intestine [9]. The main cause of SIBO is thought to be mainly bacterial. The production of enterotoxins by facultative anaerobes injures the intestinal surface while aerobic bacteria produce enzymes and metabolic products that cause epithelial injury. Symptoms of SIBO result mainly from malabsorption, and these symptoms include combinations of cramping, diarrhea, dyspepsia, and weight loss. In addition, anemia can result from malabsorption caused by occult blood loss, vitamin B12 deficiency, or both [9].

The diagnosis of SIBO usually starts with a clinical exam. The presence of 105 or more colony units of non-pharyngeal bacteria, usually coliforms, suggest SIBO [9]. Screening tests, which include urine ladicans, serum D-lactic acid, and the glucose breath hydrogen test, are also used. The most common of these, the glucose breath hydrogen test, works as an increase in the hydrogen production in the breath after glucose consumption usually means that there is a significant small intestine bacterial fermentation of carbohydrates [9].

Treatment is usually given when the symptoms become too severe, and they usually depend on the species of bacteria involved and the severity of the symptoms. Most treatments include the use of oral antibiotics, which reduce the number of bacteria in the intestinal tract. Surgery may be needed for anatomical anomalies such as diverticula, which are pouches in the colon that provide bacteria with a safe area in which to multiply. The most popular antibiotic used is metronidazole [9]. Trimethoprim-sulfamethoxazole, aminoglycosides, spectrum penicillins, and cephlasporins are usually used against facultative anaerobes [9]. If a patient has increased symptomatic activity, increased dosage may be administered. Sulfasalazine or corticosteroids may be used if there is an increased inflammatory response, however, dosage is based only on bacterial response [9].

A new and upcoming treatment used to combat SIBO is the use of bacterial substitution, which utilizes probiotic bacteria to replace existing bacteria, since the probiotic bacteria can eliminate pathogenic bacterial infections [9]. These probiotic bacteria have a beneficial effect on the small intestine. The probiotic bacteria commonly used are able to control the growth of pathogenic bacteria such as Salmonella typhimurium, Shigella sp, Clostridium difficile, Ampylobacter jejuni, and Escherichia coli, and in addition, are able to offer protection against Gardnerella vaginalitis, Bacteroides bivius, Candida albicans, and Chlamydia trachomatis. Lactic acid bacteria such as Lactobacillus GG and Actobacillus plantarum 299V are able to inhibit many gram-negative bacteria by removing toxic substances in the small intestine and stimulating the immune system [9].

Other Diseases

Crohn's Disease[2]

Salmonellosis[3]

Irritable Bowel Syndrome[4]

Current Research and Discoveries

1. Proliferation and Apoptosis of the Enterocyte is influenced by Bacteria

Prior studies have shown that the small intestines have undergone morphological changes, such as the crypt depth and villous height, after inoculating germ-free pigs with different types of bacteria. Two gnotobiotic experiments were performed where 16 piglets were allocated into 4 types of treatment groups: Germ-Free, monoassociation with Lactobacillus fermentum, Escherichia coli, or sow feces. The piglets were reared for 14 days of age where the intestinal tissue and enterocytes were collected each day for histology, gene expression, and protein analysis. Quantitative PCR was used to measure proliferating nuclear cell antigen and it was concluded that the Escherichia coli and not the Lactobacillus fermentum helped stimulate an increase apoptosis and cell proliferation. Thus, only by the death of the receptors and commensal bacteria were the enterocyte able to have a significant turnover[3].

2. Commensal Bacteria increase invasion of intestinal epithelium

Researchers from Harvard Medical School have discovered that the bacteria in the small intestine may help promote the invasion of typhoid. The beneficial bacteria located in the small intestine produce a compound that assist in redistributing the protein on the cell’s surface, which resides on the lining of intestine. This triggers epithelial cell trafficking of a protein, therefore serving as a receptor for the pathogenic bacteria. As a result, the cells become more susceptible to infection of Salmonella enterica serovar Typhi[4].

Streptococcus gordonii observed under Transmission electron micrograph .[27

3. Diversity of Intestinal Bacterial in Maturing Chicken

Bacterial flora in the ilea and ceca of chickens are analyzed by studying 1,230 partial 16S rRNA gene sequences. Bacterial DNA was isolated using density gradient centrifugation. Studies show that the microbes that live in the ileum consist of: 68.5% Lactobacillus, 11% Clostridiaceae, 6.5% Streptococcus, and 6.5% Enterococcus. The large amount and variety of bacteria in the intestine make it difficult to distinguish the function of each one; however, overall that intestinal bacterial affect: nutrition, immune responses, pathogenesis of intestinal disease, and degradation of mucus. The bacterial community in birds is affected by diet, age, and antibiotics. Overall, results showed that as the bacterial community started out as a stable community in the ileum and cecum. However, as the chickens matured, the bacterial community becomes more complex and increasingly differs between the ileum and cecum, which shows that each region develops its own unique set of microbes[16].

4. Regulation of Intestinal Angiogenesis by Microbes via Paneth Cells

Using three-dimensional imaging, researchers at the Washington University School of Medicine studied the impact of intestinal angiogenesis on the microbes in the small intestine of mice. Angiogenesis, the growth of new blood vessels, takes place in the complex network of blood vessels in small intestinal villi. However, the villi is also the site where gut microorganisms, or microbiota, live. This experiment studies the differences between the villi in germ-free mice and mice with Bacteroides thetaiotaomicron colonies during or after postnatal gut development – approximately a ten day period. Studies have shown that Bacteroides thetaiotaomicron is of the major microbes that reside in both mouse and human gut. This experiment focuses on Paneth cells, which defend the host against microbes by secreting antibacterial peptides that affect the microbes in the lumen, or interior of the blood vessels. The results show that microbes function in breaking down carbohydrate polymers by facilitating lumen breakdown of dietary macromolecules so that the host does not have to cleave the various linkages in their food themselves. Symbiosis is observed since the host is able to gain more nutrients, while microbes are provided a safe niche with a plentiful carbon source[17].

5. Microbial Symbiosis prevents Intestinal Inflammatory Disease

Symbiosis between microbes and their human host can be observed by microbes preventing intestinal inflammatory disease. One of the main microbes this experiment focuses on is Bacteroides fragilis, which protects its human host against colitis, a chronic inflammation of the membrane lining the gastrointestinal tract, caused by Helicobacter hepaticus, a commensal bacterium. Although colitis is mainly affects the large intestine, in some rare cases it affects the ileum as well since it precedes the large intestine. In order for Bacteroides fragilis to be beneficial to its host, it must express polysaccharide A, or PSA, which affects the interleukin-10-producing CD4 T cells; otherwise Helicobacter hepaticus will continue to grow and cause inflammatory disease. It is shown that purified PSA can prevent gut pathology. This experiment gives purified PSA to mice and measures the changes of the inflammatory disease. Mice without Helicobacter hepaticus had a very mild colitis, while mice with Helicobacter hepaticus developed severe colitis. When purified PSA was given to the diseased mice, they were almost completely protected against the Helicobacter hepaticus, and the disease level was reduced to match those who did not develop colitis. Thus, Bacteroides fragilis is just one example of a symbiosis relationship where a single bacterial organism promotes human health; and suggests that there are others yet to be discovered[18].

6. Effects of Dietary Fat Source and Antibiotics and Age on Bacterial in the Ileum of Chickens

PCR with denaturing gradient gel electrophoresis (DGGE) studies how different fat sources in the diets of broiler chickens affected the microbial community in the ileum of the chickens at different ages. DGGE involves molecular fingerprinting which separates polymerase chain reaction (PCR)-generated DNA products. This experiment differs from previous experiments, which only focused on the cecum, by examining the parts of the small intestine – the jejunum and ileum, which are largely responsible for nutrient absorption. The experiment focuses only on the ileum, but notes that the same micro flora exist in both sites. In addition, instead of studying the effects of dietary carbohydrate sources, it focuses on dietary fat sources by feeding the chickens with either soy oil or lard and tallow mix and an antibiotic supplement (avilamycin and salinomycin mix). Salinomycin antibiotic inhibits the growth of C. perfringens. The results confirmed that the microbial communities were affected by both factors: the chickens’‘ dietary fat source with antibiotics as well as the age of the chickens. As the chickens aged, there was an increase in Streptococcus alactolyticus, enterobacteria, and Clostridium perfringens. The different dietary fat sources primarily affected Lactobacillus salivarius and Clostridium perfringens. Results show that soy oil has a higher solubility which help the dispersion of antibiotics in the small intestine; thus, indirectly affects the microbial communities that live there. Clostridium perfringens, which was affected by both age and dietary fat sources, also exhibited different strains in response to the factors[19].

7. Why the immune system doesn’t attack the small intestine

Researchers from the Dana Farber Cancer Institute and Shannon Turley, PhD, recently identified a group of lymph node cells that instruct the immune system to leave healthy tissue alone. They have found that cells that are not generally thought of as part of the immune system actually help protect against microbe attacks in the intestine. Since the lymph nodes are found throughout the body, they suppress a variety of autoimmune diseases, which normally results from immune system assault on healthy tissue. In order to distinguish the normal and foreign cells, small proteins are located on the cell surface called antigens. Thus the dendritic cells exposed the antigens of normal neighboring cells, which in-turn puts the immune system at ease. Turley discovered that dendritic cells are not essential in creating tolerance in T cells but instead tolerance is produced by stomal cells from nearby lymph nodes[24].

Conclusion

A wide variety of microbes reside on the mucus surfaces of the small intestine. From these 500 bacterial species, many of these microbial communities are beneficial for the host by: aiding in nutrient absorption, stimulating growth, providing protection against pathogens, producing antibiotics, and even aiding in muscular activity of the small intestine. However, some microbes are parasitic and cause diseases. Despite the large number and diversity of microbes that inhabit the small intestine, current research and studies on the small intestine gradually provide more insight on the specific function each microbe, which can lead to the development of new drugs and treatments for diseases.

References

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[4] Lyczak, J.B. "Commensal Bacteria Increase Invasion of Intestinal Epithelium by Salmonella enterica Serovar Typhi." INFECTION AND IMMUNITY 71 (2003): 6610-614.

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[9] Vanderhoof, Jon A., Rosemary J. Young, Nancy Murray, and Stuart S. Kaufman. "Treatment Strategies for Small Bowel Bacterial Overgrowth in Short Bowel Syndrome." Journal of Pediatric Gastroenterology & Nutrition 27 (1998): 155-60.

[10]http://www.textbookofbacteriology.net/normalflora.html

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[15]Nagi/Babiuk, A. M. "Preparation, purification and characterization of bovine Peyer's patch leukocytes." PudMed Central. Apr. 1988. Canadian Veterinary Medical Association. 29 Aug. 2008 <http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1255435>.

[16]Lu, Jiangrang, Umelaalim Idris, Barry Harmon, Charles Hofacre, John J. Maurer, and Margie D. Lee. ‘‘Diversity and Succession of the Intestinal Bacterial Community of the Maturing Broiler Chicken.’‘ Applied and Environmental Microbiology 11th ser. 69 (2003): 6816-824.

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Edited by Ellis Chiu, Janet Kwok, Kevin Lee, Susan Lee, Martin Tang, Adnan Vahora, students of Rachel Larsen

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