Thermus

From MicrobeWiki, the student-edited microbiology resource

Jump to: navigation, search
This is a curated page. Report corrections to Microbewiki.

A Microbial Biorealm page on the genus Thermus

Strains of thermophilic species isolated from the Tanganyika Lake. The left sample is an example of a vesicular strain while the sample at right shows a more "rotund" bodied cell. From the Tangandro Expedition.

Contents

Classification

Higher order taxa:

Bacteria; Deinococcus-Thermus; Deinococci; Thermales; Thermaceae

Species:

T. antranikianii, T. aquaticus, T. brockianus, T. caldophilus, T. filiformis, T. igniterrae, T. kawarayuensis, T. nonproteolyticus, T. oshimai, T. rehai, T. scotoductus, T. thermophilus,T. thermophilus HB27, T. yunnanensiT. sp., T. sp. manikaranii , T. spp.

NCBI: Taxonomy Genome

Description and Significance

Thermus bacteria cells are a Gram-negative bacteria and are "either immotile or possess flagella and are motile" (R. Huber et al. 2004). Thermus is both more active at higher temperatures and more thermostable than most other enzymes. Go here to learn about Thermus thermophilus in vitamin B(12) synthesis.

Genome Structure

Thermus is assumed to have a pilus-like structure for conjugation, though the molecular basis of the DNA uptake is still unknown. In the three loci of the T. thermophilus genome, twelve genes encoding preplin-like proteins were found. Mutational analysis suggests that one locus (containing four encoding preplin-like protein genes) is essential for natural transformation (A. Friedrich et al. 2002).

Cell Structure and Metabolism

Thermus thermophilus. Courtesy of Francois Franceschi and the Max Planck Institute.

Thermus has an optimum growth temperature of between 70°C and 75 °C (min: 37-45°C, max: 79°C), though some have lower growth temperatures of about 60°C (min: 35-40°C, max: 70°C). High temperature Thermus pH ranges between 7.5 and 8.0, though strains grow as low as 5.1 and as high as 9.5 (R. Huber et al. 2004 ). No strain of Thermus appears to be capable of fermentation.

Thermus strains use several substrates for growth, including carbohydrates, amino acids, carboxylic acids, and peptides (R. Huber et al. 2004). Because of the diversity in habitat from which Thermus strains are isolated, the nutritional diversity also has a large variety. Thermus strains often use monosaccharides as a source for single carbons, though typically pentoses is not metabolized. Thermus strains do not necessarily use the constituent monosaccharide of the disaccharides being used. Most Icelandic strains use sucrose and maltose, though two strains use glucose. The proteins hydrolyzed vary by the isolates as well; while elastin, fibrin, and casein are used by many high-temperature strains of Thermus, nitrate is used as a terminal electrany strains, some are unable to hydrolyze each substrate (R. Huber et al. 2004). For mon acceptor and Themus grows anaerobically in the presence (but not in the absence) of it. Metabolic studies have shown Thermus aquaticus to be a complete tricarboxylic acid cycle, giving a glyoxylate bypass as well with isocitrate lyase and malate synthase (Pask-Hughes).

Ecology

Thermus strains are found in hot springs in the enzyme Taq, used for DNA fingerprinting. From the Lunar and Planetary Institute.

Thermus-like bacteria have been found in both shallow and deep-sea marine hydrothermal systems, as well as in low saline solfataric springs. The Thermus strain T. aquaticus was first isolated in Yellowstone National Park from neutral and alkaline springs and has since then also been isolated from hot springs found in Iceland. Currently, they have been isolated from high temperature (55-100°C) and weak acid to alkaline pH (5-9) habitats, though strains of Thermus were isolated from sites as acidic as pH 3.9 in New Zealand (R. Huber et al. 2004).

References

Alfredsson G. A. ; J. K. Kristjansson; S. Hjorleifsdottir; K. O. Stetter; 1988. "Rhodothermus marinus, gen. nov., sp. nov., a thermophilic, halophilic bacterium from submarine hot springs in Iceland." J. Gen. Microbiol. vol. 134, pp. 299–306.

Friedrich, A., J. Rumszauer, A. Henne, B. Averhoff. "Pilin-Like Proteins in the Extremely Thermophilic Bacterium Thermus thermophilus HB27: Implication in Competence for Natural Transformation and Links to Type IV Pilus Biogenesis". Applied and Environmental Microbiology, July 2003, p. 3695-3700, Vol. 69, No. 7.

Huber, R., K. O. Stetter, The Prokaryotes: An Evolving Electronic Resource for the Mircobiological Community. 2004. Springer-Verlag New York, LLC.

Lunar and Planetary Institute. 2002. Yellowstone Hot Springs Expedition.

Pask-Hughes R. A. ; R. A. D. Williams. "Extremely thermophilic gram-negative bacteria from hot tap water." Journal of general microbiology. 1975 88: 321-328

Rehse, P.H.; Kitao, T.; Tahirov, T.H. "Acta Crystallography." (2005). D61, 913-919.

Tangandro Expedition. 1996. "Tangandro." Institut de Génétique et Microbiologie.

Williams, R. A. D., M. S. Da Costa, The Prokaryotes: An Evolving Electronic Resource for the Mircobiological Community. 2004

Personal tools