Tina Torres Bacillus thuringiensis

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Classification

Domain: Bacteria, Phylum: Firmicutes, Class: Bacilli, Order: Bacillales, Family: Bacillaceae [Others may be used. Use NCBI link to find]

Species

NCBI: Taxonomy

Bacillus thuringiensis

Habitat Information

This soil sample was collected at 289 Spring Lane, Dripping Springs, TX 78620 Temperature: 60 F Humidity: 40% Wind Speed: NE 14G 22 mph Dewpoint: 35 F GPS coordinates:

Description and Significance

When streaked on an LB plate, the colonies that formed were opaque in appearance and flat. When tested for antimicrobial properties the one antibiotic that showed the most susceptibility was Sulfisoxazole, also a small zone of inhibition was seen with Ampicillin. Linezolid and Cefamandole showed no zone of inhibition.

Bacillus thuringiensis is a gram positive, soil dwelling bacterium that is commonly used as a biological pesticide.

[File:LB_Streak_Plate.jpg]

Genome Structure

This is the forward sequence I used to determine that my soil sample was Bacillus thuringiensis:

GACGGAGCAACGCCGCGTGAGTGATGAAGGCTTTCGGGTCGTAAAACTCTGTTGTTAGGGAAGAACAAGTGCTAGTTGAATAAGCTGGCACCTTGACGGTACCTAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGCGCAGGTGGTTTCTTAAGTCTGATGTGAAAGCCCACGGCTCAACCGTGGAGGGTCATTGGAAACTGGGAGACTTGAGTGCAGAAGAGGAAAGTGGAATTCCATGTGTAGCGGTGAAATGCGTAGAGATATGGAGGAACACCAGTGGCGAAGGCGACTTTCTGGTCTGTAACTGACACTGAGGCGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTAGAGGGTTTCCGCCCTTTANTGCTGAAGTTAACGCATTAAGCACTCCGCCTGGGGAGTACGGCCGCAAGGCTGAAACTCNNAGGAATTGACNGGGGCCCGCACAANCGGTGGANCATGTGGTTTAATT ACCAGGTNTTGAAATCCTCTGANAACCCTANAGATACGGCNTCTCNCNTCTNNAACATANTGAC

Consists of a 5.5-Mb chromosome and nine plasmids.

This organism is gram positive and forms endospores.

It is also found naturally in the gut of caterpillars, moths and butterflies.

Cell Structure, Metabolism and Life Cycle

This gram positive microorganism has a thick, cross linked peptidoglycan layer in the bacterial cell wall. It is harmful to many insects, it works by

Interesting features of cell structure; how it gains energy; what important molecules it produces.

Physiology and Pathogenesis

Biochemical characteristics, enzymes made, other characteristics that may be used to identify the organism; contributions to environment (if any).
If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.

Using various biochemical tests I was able to determine the following about this organism:

Citrate: test used to test an organism's ability to use carbon as it's only source

        *soil sample - negative for citrate

SIM (Sulfar, Indole, Motility)

        *soil sample - negative for sulfar, indole and motility

Nitrate

        *soil sample - positive for nitrite reduction (nitrate --> nitrite)

Urea: tests an organism's ability to break down or convert urea to amonia

        *soil sample - negative for urea

TSI (Triple Sugar Iron)

        *soil sample - glucose fermentation with acid production

Decarboxylation: tests organism's ability to produce an enzyme called decarboxylase

        *soil sample - Argine: positive for decarboxylase
                       Lysine: positive for fermentation
                       Ornithine: positive for decarboxylase

Phenylalanine Deaminase: tests organism's ability to produce enzyme deaminase

        *soil sample - negative

Oxidase: identifies organisms that produce enzyme cytochrome oxidase, which participates in the electron transport chain

        *soil sample - positive for cytochrome oxidase

Hektoen Enteric Agar: this media is selective and differential that is used to isolate Salmonella and Shigella species

        *soil sample - negative and negative for fermentation

MacConkey Agar: selective and differential media that is used to isolate organisms based on their ability to ferment lactose

        *soil sample - negative and negative for fermentation

Eosin Methylene Blue Agar: selective and differential media used to isolate fecal coliforms

        *soil sample - positive for fermentation

References

[Sample reference] Takai, K., Sugai, A., Itoh, T., and Horikoshi, K. "Palaeococcus ferrophilus gen. nov., sp. nov., a barophilic, hyperthermophilic archaeon from a deep-sea hydrothermal vent chimney". International Journal of Systematic and Evolutionary Microbiology. 2000. Volume 50. p. 489-500.

Author

Page authored by _____, student of Prof. Kristine Hollingsworth at Austin Community College.