T7 Bacteriophage: Difference between revisions
(Created page with "T7 Bacteriophage - Enterobacteria phage T7 Classification Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Autographiviridae; Studiervirinae; Teseptimavirus Species Teseptimavirus T7 Description and Significance Like other viruses, the T7 bacteriophage does not produce its own energy. Instead, it infiltrates a bacterium via a membrane protein, and alters the genome in the nuclear envelope. By doing this, the T7 bacteriophage interferes with the...") |
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{{T7 Bacteriophage - Enterobacteria phage T7}} | |||
[[Image:T7Phage.jpg|thumb|300px|right|Legend. Image credit: Wikipedia.]] | |||
==Classification== | |||
Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Autographiviridae; Studiervirinae; Teseptimavirus [Others may be used. Use [http://www.ncbi.nlm.nih.gov/Taxonomy/ NCBI] link to find] | |||
Genome Structure | |||
===Species=== | |||
{| | |||
| height="10" bgcolor="#FFDF95" | | |||
'''NCBI: [https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=1007084&lvl=3&lin=f&keep=1&srchmode=1&unlock]''' | |||
|} | |||
''Genus species'' | |||
==Description and Significance== | |||
Describe the appearance, habitat, etc. of the organism, and why you think it is important. | |||
==Genome Structure== | |||
The genome of the T7 Bacteriophage is comprised of approximately 40,000 bp of DNA coding for 55 proteins. Many of these genes are overlapping and are removed via refactoring of the genome. | The genome of the T7 Bacteriophage is comprised of approximately 40,000 bp of DNA coding for 55 proteins. Many of these genes are overlapping and are removed via refactoring of the genome. | ||
Structure and Life Cycle | |||
==Cell Structure, Metabolism and Life Cycle== | |||
The T7 Bacteriophage possesses an icosahedral capsid shell made by gene product 10, and a 12-fold portal, a feature commonly found in tailed bacteriophages and herpesviruses. (Chen, Xiao, Wang, Cheng 2021) The T7 bacteriophage has a tail with a 12-fold adaptor protein gp11 assembly, a sixfold nozzle protein gp12 assembly, and six subunits of trimeric tail fiber gp17. The tail fibers of the T7 bacteriophage are used to recognize potential hosts, and absorb into them. On top of the portal, the hollow capsid shell can be found. | The T7 Bacteriophage possesses an icosahedral capsid shell made by gene product 10, and a 12-fold portal, a feature commonly found in tailed bacteriophages and herpesviruses. (Chen, Xiao, Wang, Cheng 2021) The T7 bacteriophage has a tail with a 12-fold adaptor protein gp11 assembly, a sixfold nozzle protein gp12 assembly, and six subunits of trimeric tail fiber gp17. The tail fibers of the T7 bacteriophage are used to recognize potential hosts, and absorb into them. On top of the portal, the hollow capsid shell can be found. | ||
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The life cycle of a T7 bacteriophage consists of three primary steps. Firstly, the phage searches for and absorbs into a host; in the second step, the phage transfers its linear DNA genome into a host cell and expresses its phage genes. Third, the host cell is lysed and releases the newly assembled phages. (Nguyen, Kang 2014) On the surface, the T7 bacteriophage targets a lipopolysaccharide receptor, which binds to the C-terminal end of the T7 tail fiber protein. (Nguyen, Kang 2014) The DNA of the phage is translocated into the host in a process dependent on transcription, which is performed by the hosts RNA polymerase and then by the T7 phages RNA polymerase. | The life cycle of a T7 bacteriophage consists of three primary steps. Firstly, the phage searches for and absorbs into a host; in the second step, the phage transfers its linear DNA genome into a host cell and expresses its phage genes. Third, the host cell is lysed and releases the newly assembled phages. (Nguyen, Kang 2014) On the surface, the T7 bacteriophage targets a lipopolysaccharide receptor, which binds to the C-terminal end of the T7 tail fiber protein. (Nguyen, Kang 2014) The DNA of the phage is translocated into the host in a process dependent on transcription, which is performed by the hosts RNA polymerase and then by the T7 phages RNA polymerase. | ||
References | |||
==Ecology and Pathogenesis== | |||
Habitat; symbiosis; biogeochemical significance; contributions to environment.<br> | |||
If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.<br><br> | |||
==References== | |||
U.S. National Library of Medicine. (n.d.). Taxonomy browser (escherichia phage T7). National Center for Biotechnology Information. https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=10760&lvl=3&lin=f&keep=1&srchmode=1&unlock | U.S. National Library of Medicine. (n.d.). Taxonomy browser (escherichia phage T7). National Center for Biotechnology Information. https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=10760&lvl=3&lin=f&keep=1&srchmode=1&unlock | ||
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Chen, W., Xiao, H., Wang, L., & Cheng, L. (2021, September 9). Structural changes in bacteriophage T7 upon receptor-induced ... - PNAS. PNAS. https://www.pnas.org/doi/full/10.1073/pnas.2102003118 | Chen, W., Xiao, H., Wang, L., & Cheng, L. (2021, September 9). Structural changes in bacteriophage T7 upon receptor-induced ... - PNAS. PNAS. https://www.pnas.org/doi/full/10.1073/pnas.2102003118 | ||
==Author== | |||
Page authored by Zachary Lovvorn, student of Prof. Bradley Tolar at UNC Wilmington. | |||
<!-- Do not remove this line-->[[Category:Pages edited by students of Bradley Tolar at UNC Wilmington]] | |||
Revision as of 23:20, 11 December 2023
Template:T7 Bacteriophage - Enterobacteria phage T7
Classification
Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Autographiviridae; Studiervirinae; Teseptimavirus [Others may be used. Use NCBI link to find]
Species
|
NCBI: [1] |
Genus species
Description and Significance
Describe the appearance, habitat, etc. of the organism, and why you think it is important.
Genome Structure
The genome of the T7 Bacteriophage is comprised of approximately 40,000 bp of DNA coding for 55 proteins. Many of these genes are overlapping and are removed via refactoring of the genome.
Cell Structure, Metabolism and Life Cycle
The T7 Bacteriophage possesses an icosahedral capsid shell made by gene product 10, and a 12-fold portal, a feature commonly found in tailed bacteriophages and herpesviruses. (Chen, Xiao, Wang, Cheng 2021) The T7 bacteriophage has a tail with a 12-fold adaptor protein gp11 assembly, a sixfold nozzle protein gp12 assembly, and six subunits of trimeric tail fiber gp17. The tail fibers of the T7 bacteriophage are used to recognize potential hosts, and absorb into them. On top of the portal, the hollow capsid shell can be found.
The life cycle of a T7 bacteriophage consists of three primary steps. Firstly, the phage searches for and absorbs into a host; in the second step, the phage transfers its linear DNA genome into a host cell and expresses its phage genes. Third, the host cell is lysed and releases the newly assembled phages. (Nguyen, Kang 2014) On the surface, the T7 bacteriophage targets a lipopolysaccharide receptor, which binds to the C-terminal end of the T7 tail fiber protein. (Nguyen, Kang 2014) The DNA of the phage is translocated into the host in a process dependent on transcription, which is performed by the hosts RNA polymerase and then by the T7 phages RNA polymerase.
Ecology and Pathogenesis
Habitat; symbiosis; biogeochemical significance; contributions to environment.
If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.
References
U.S. National Library of Medicine. (n.d.). Taxonomy browser (escherichia phage T7). National Center for Biotechnology Information. https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=10760&lvl=3&lin=f&keep=1&srchmode=1&unlock
Nguyen, H. M., & Kang, C. (2014, February). Lysis delay and burst shrinkage of coliphage T7 by deletion of Terminator Tφ reversed by deletion of early genes. Journal of virology. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3911561/#:~:text=The%20replication%20cycle%20of%20phage,release%20newly%20assembled%20phage%20progenies.
Chen, W., Xiao, H., Wang, L., & Cheng, L. (2021, September 9). Structural changes in bacteriophage T7 upon receptor-induced ... - PNAS. PNAS. https://www.pnas.org/doi/full/10.1073/pnas.2102003118
Author
Page authored by Zachary Lovvorn, student of Prof. Bradley Tolar at UNC Wilmington.
