Ralstonia eutropha: Difference between revisions

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==Description and significance==
==Description and significance==


Thermoplasma volcanium can be isolated from coal refuse piles, solfatara fields, and hot springs.  This microbe is thermophilic and acidophilicIt lives in a high temperature environment in the range of 33˚C to 67˚C with the optimum at 60˚CEven though it survives at this high temperature, it is still the lowest among archaea. Additionally, it only survives in acidic environment with pH between 1.0 and 4.0, with the optimum at pH of 2.0.  Thermoplasma cells lyse at neutral pH.  Research has shown that Thermoplasma volcanium may be the host cell of the endosymbrosis theory of eukaryotic cells.  Hence, the genome is sequenced to confirm this hypothesis.
Ralstonia eutropha JMP 134 can be found in both soil and water.  This bacterium has great potential for use in bioremediation as it is able to degrade an great number of chlorinated aromatic (chloroaromatic) compounds and chemically related pollutantsRalstonia eutropha H16 was formerly known as Alcaligenes eutrophus which was originally isolated from sludgeRalstonia eutropha JMP 134 and other strains belonging to this species are models for studying microbial production of “polyhydroxyalkanoates, and chemolithoautotrophic metabolism in aerobic heterotrophs.(JGI)”
Ralstonia eutropha strains can utilize hydrogen, carbon dioxide and organic compounds for development and is model organism for hydrogen oxidation because it can nurture on hydrogen as the sole energy source. Ralstonia eutropha JMP 134 was isolated dues to its ability to degrade the herbicide 2,4-dichlorophenoxyacetic acid, which was encoded on plasmid as having the function of degrading.


==Genome structure==
==Genome structure==

Revision as of 05:32, 5 June 2007

A Microbial Biorealm page on the genus Ralstonia eutropha

Classification

Higher order taxa

Domain:Bacteria

Phylum:Proteobacteria

Class: Betaproteobacteria

Order: Burkholderiales

Family:Burkholderiaceae

Genus: Cupriavidus/Ralstonia/Wautersia

Species

NCBI: [1]

Genus Species synonym and strains:

1. Cupriavidus necator

2. Wautersia eutropha

3. Ralstonia eutropha

    a. Ralstonia eutropha JMP 134
    b. Ralstonia eutropha H850
    c. Ralstonia eutropha H16 

Description and significance

Ralstonia eutropha JMP 134 can be found in both soil and water. This bacterium has great potential for use in bioremediation as it is able to degrade an great number of chlorinated aromatic (chloroaromatic) compounds and chemically related pollutants. Ralstonia eutropha H16 was formerly known as Alcaligenes eutrophus which was originally isolated from sludge. Ralstonia eutropha JMP 134 and other strains belonging to this species are models for studying microbial production of “polyhydroxyalkanoates, and chemolithoautotrophic metabolism in aerobic heterotrophs.(JGI)” Ralstonia eutropha strains can utilize hydrogen, carbon dioxide and organic compounds for development and is model organism for hydrogen oxidation because it can nurture on hydrogen as the sole energy source. Ralstonia eutropha JMP 134 was isolated dues to its ability to degrade the herbicide 2,4-dichlorophenoxyacetic acid, which was encoded on plasmid as having the function of degrading.

Genome structure

Thermoplasma volcanium has a circular DNA with 1,584,804 nucleotides. It does not contain any plasmids. However, it possesses about 70 proteins not found in any other archaea’s genome.

Cell structure and metabolism

This microbe has a unique cell membrane that contains tetraether lipids. It lacks any kind of cell wall, which causes it to have irregular shapes and is capable of assuming different shapes. The microbe uses multiple flagella for high motility. Thermoplasma volcanium is heterotrophic and therefore requires it to obtain nutrients from other organisms especially those who cannot survive in acidic or high temperature environments. Depending on its living conditions, the microbe is both anaerobic and aerobic. It is anaerobic in the presence of elemental sulfur.

Ecology

Due to its evolutionary ties to eukaryotes, Thermoplasma genus can be used as model organism for researches.

Pathology

There is no known pathogen among different strains of Ralstonia eutropha.

Application to Biotechnology

Does this organism produce any useful compounds or enzymes? What are they and how are they used?

Current Research

Enter summaries of the most recent research here--at least three required

References

[JGI] [2]

Edited by student of Rachel Larsen and Kit Pogliano