Soil Sample Number 13: Difference between revisions
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Revision as of 02:56, 16 November 2015
Classification
Domain: Bacteria; Phylum: Firmicutes; Class: Bacilli; Order: Bacillales; Family: Bacillaceae; Genus: Bacillus thermocopriae
Species
Bacillus thermocopriae
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NCBI: Taxonomy |
The soil was collected from a pasture on my father's farm in Lexington, TX at 7:04 pm on September 2, 2015. The weather was dry and 89 degrees F and there had been zero rain in the past 7 days. The soil was collected in a shady area at the back of the pasture along the fence line from about 2 inches beneath the surface and kept at room temperature on the kitchen counter for 2 days.
Description and Significance
Describe the appearance (colonial and cellular), possible antimicrobial activity etc. of the organism, and why the organism might be significant.
Soil sample number 13 when cultured on LB Agar is white, opaque, round, regular, small, shiny. It is found to be gram positive rod and is in a strong chain formation. The endospore stain revealed that this sample does have endospores. When tested for zone of inhibitions against E. coli and S. aureus there were no zones. Endospores make the sample extremely resistant to drying, heat, lethal chemicals and radiation.
Genome Structure
Describe the size and content of the genome. How many chromosomes? Circular or linear? Other interesting features? What is known about its sequence? Include S Ribosomal sequence that you obtained from PCR and sequencing here.
16S Ribosomal sequence
>DLF12-Reverse_D11.ab1ACCACCTGTCACTCTGTCCCCCGAAGGGGAAATTCCTATCTCTAGGAGTGTCAGAGGA
TGTCAAGACCTGGTAAGGTTCTTCGCGTTGCTTCGAATTAAACCACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTC
CTTTGAGTTTCAGCCTTGCGGCCGTACTCCCCAGGCGGAGTGCTTAATGCGTTAGCTGCAGCACTAAAGGGCGGAAACCC
TCTAACACTTAGCACTCATCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTC
AGCGTCAGTTACAGACCAGAAAGCCGCCTTCGCCACTGGTGTTCCTCCACATCTCTACGCATTTCACCGCTACACGTGGA
ATTCCGCTTTCCTCTTCTGCACTCAAGTCCCCCAGTTTCCAATGACCCTCCACGGTTGAGCCGTGGGCTTTCACATCAGA
CTTAAAGGACCGCCTGCGCGCGCTTTACGCCCAATAATTCCGGACAACGCTTGCCACCTACGTATTACCGCGGCTGCTGG
CACGTAGTTAGCCGTGGCTTTCTGGTTAGGTACCGTCAAGGTACCGGCAGTTACTCCGGTACTTGTTCTTCCCTAACAAC
AGAGCTTTACGACCCGAAGGCCTTCATCGCTCACGCGGCGTTGCTCCATCAGACTTTCGTCCATTGTGGAAGATTCCCTA
Cell Structure, Metabolism and Life Cycle
Interesting features of cell structure; how it gains energy; what important molecules it produces.
Most closely related to Bacillus drentensis.
Physiology and Pathogenesis
Biochemical characteristics, enzymes made, other characteristics that may be used to identify the organism; contributions to environment (if any).
If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.
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Soil sample number 13 tests positive for Glucose and Sucrose but is negative for Lactose when tested with Phenol Red Broth. It is positive for starch hydrolysis. When the casein hydrolysis test was performed nothing grew. After repeating the test still nothing grew. It was negative for gelatin hydrolysis as the tube remained solid and did not liquify. The DNA hydrolysis test was negative. Soil Sample number 13 was positive for lipid hydrolysis. The MR and the VP tests both were negative. It Simmons Citrate slant remained green which indicated a negative result for citrate. The SIM test resulted in a negative for motility, sulfur reduction and indole production from tryptophan. Our soil was positive for nitrate reduction. It also tested positive on the Triple Iron Sugar test for fermentation with acid accumulation. Soil sample number 13 was negative for Urea hydrolysis. Positive results for Arginine, Lysine and Ornithine as well as the Oxidase test were observed as well. The phenylalanine deaminase test was observed as a negative result. There was no growth on the EMB, HE and MAC plates which means the soil is negative for all of these tests as well. It is positive for Catalase.
References
ijs.microbiologyresearch.org/content/journel/ijsem/10.1099/ijs.0.046953-0
http://ncbi.nlm.nih.gov/Taxonomy/NCBI
Author
Page authored by Erin Andrews and Tabitha Sherer, students of Prof. Kristine Hollingsworth at Austin Community College.
