https://microbewiki.kenyon.edu/api.php?action=feedcontributions&user=Bgalindo6106&feedformat=atommicrobewiki - User contributions [en]2024-03-29T06:33:10ZUser contributionsMediaWiki 1.39.6https://microbewiki.kenyon.edu/index.php?title=Moritella_marina&diff=127577Moritella marina2016-12-03T05:23:12Z<p>Bgalindo6106: /* References */</p>
<hr />
<div>=Classification=<br />
Domain: Bacteria<br />
<br />
Phylum: Proteobacteria<br />
<br />
Class: Gammaproteobacteria<br />
<br />
Order: Alteromonadales<br />
<br />
Family: Moritellaceae<br />
<br />
Genus: Moritella<br />
<br />
=Species=<br />
''M. japonica, M. yayanosii, M. viscosa, M. profunda, M. abyssi, M. dasanesis''<br />
<br />
<br />
=Description and Significance=<br />
''Moritella marina'' is a gram-negative halophilic psychrophilic facultative anaerobe with curved or straight rods, motile polar flagella that produce PUFA’s (polyunsaturated fatty acids) and DHA (docosahexanoic acid). “Twenty-five years ago, a marine bacterium originally designated ''Vibrio marinus'' and later renamed ''Moritella marina'' MP-1 was reported to produce high levels of DHA (18% of the total fatty acids). Such high levels of DHA presumably provide this bacterium with the ability to maintain appropriate membrane fluidity in the low temperatures and high pressures of its marine environment.”<br />
“Phylogenetic tree of some representatives of the γ-subclass of the class Proteobacteria showing the position of 16S rDNA sequences from some ''Alteromonas macleodii''-related strains and clone sequences from picoplankton DNA from the Mediterranean Sea mesocosm experiment. Sequences of clones 17B161 and 1B161 are available under accession number Y18229 and Y18231, respectively. The tree was calculated by the algorithm of De Soete [16]. The percentage of 500 bootstrap samplings that support branching points above 80% confidence are indicated. The scale bar corresponds to two nucleotide substitutions over 100 sequence positions. The tree was rooted with ''Roseobacter algicola'' as an outgroup.” (FEMS, 1998)<br />
<br />
[[image:Phytree.png|thumbnail|300px| Figure 1: The Family ''Moritellacae''(Urakawa, 2014). ''Moritella viscosa'' is 99% closely related to ''Moritella marina.'' ]]<br />
<br />
=16S Ribosomal RNA Gene Information=<br />
<br />
<br />
[[image:16s.png|thumbnail|300px| Figure 2: Image credit:(Urakawa, 2014)]]<br />
<br />
“Sequence variation at positions 73–98 and 181–214 (E. coli nomenclature) within two variable regions of the 16S rDNA of representatives of clone clusters 1–5 and some strains isolated from mesocosm B. Probe target regions are in bold” (FEMS,1998)<br />
<br />
=Genome Structure=<br />
<br />
Look at Figure: 3<br />
<br />
[[image:GenomeStructure.png|thumbnail|350px| Figure 3: High sequence diversity of Alteromonas macleodii-related cloned and cellular 16S rDNAs from a Mediterranean seawater mesocosm experiment. (FEMS Microbiology Ecology, 2016) ]]<br />
<br />
=Ecology and pathogenesis=<br />
''Moritella marina'' have been found in a variety of cold water marine environments, ranging from the ocean floor to the intestinal tract of marine organisms. Most ''Moritella'' species are thought to live mutualistically amongst larger marine organisms, but ''Moritella viscosa'' has been noted to cause skin ulcers in some fish (Urakawa, 2014). <br />
=Current Research=<br />
''Moritella marina'' has been noted for having unusually high production of the long chain polyunsaturated fatty acids (PUFAs). When ''M. marina'' strain MP-1 was cultured in medium containing cerulenin, a fatty acid synthesis inhibitor, decreases in levels of middle-chain fatty acids and remarkable increases in levels of DHA were observed. These results suggest that the synthesis of middle-chain fatty acids works independently of the synthesis of DHA. M. marina was also found to produce chitonase when induced with chiton (Stefanidi, E., & Vorgias, C. E., 2008). <br />
=References=<br />
<br />
<br />
And, Kumar B. Kautharapu. "Kumar B. Kautharapu." ''Genome Sequence of the Psychrophilic Deep-Sea Bacterium Moritella Marina MP-1'' (ATCC 15381). N.p., 2012. Web. 02 Dec. 2016.<br />
http://jb.asm.org/content/194/22/6296.full<br />
<br />
<br />
Urakawa, H., Kita-Tsukamoto, K., Steven, S. E., Ohwada, K., & Colwell, R. R. (1998). A proposal to transfer Vibrio marinus (Russell 1891) to a new genus Moritella gen. nov. as Moritella marina comb. nov. FEMS microbiology letters, 165(2), 373-378. http://femsle.oxfordjournals.org/content/165/2/373.full#ref-13<br />
<br />
<br />
Pukall, R., Päuker, O., Buntefuß, D., Ulrichs, G., Lebaron, P., Bernard, L., ... & Stackebrandt, E. (1999). High sequence diversity of Alteromonas macleodii-related cloned and cellular 16S rDNAs from a Mediterranean seawater mesocosm experiment. FEMS Microbiology Ecology, 28(4), 335-344.<br />
http://femsec.oxfordjournals.org/content/28/4/335<br />
<br />
<br />
Stefanidi, E., & Vorgias, C. E. (2008). Molecular analysis of the gene encoding a new chitinase from the marine psychrophilic bacterium ''Moritella marina'' and biochemical characterization of the recombinant enzyme. Extremophiles, 12(4), 541-552.<br />
"FEMS Microbiology Ecology." High Sequence Diversity of Alteromonas Macleodii-related Cloned and Cellular 16S RDNAs from a Mediterranean Seawater Mesocosm Experiment | FEMS Microbiology Ecology. N.p., n.d. Web. 02 Dec. 2016.<br />
http://femsec.oxfordjournals.org/content/28/4/335 <br />
<br />
<br />
http://www.bacterio.net/moritella.html <br />
<br />
<br />
Genome structure image:<br />
https://www.patricbrc.org/portal/portal/patric/CircosGenomeViewer?cType=genome&cId=1202962.4 <br />
<br />
<br />
http://jb.asm.org/content/194/22/6296.full.pdf<br />
<br />
http://lib.dr.iastate.edu/cgi/viewcontent.cgi?article=1177&context=cbe_pubs<br />
<br />
http://www.biochemsoctrans.org/content/28/6/943.long<br />
<br />
https://www.researchgate.net/publication/232721452_Genome_Sequence_of_the_Psychrophilic_Deep-Sea_Bacterium_Moritella_marina_MP-1_ATCC_15381<br />
<br />
http://aem.asm.org/content/51/4/730.abstract?ijkey=6cfa0071e9fc15e0a007123a571561c86c1354d3&keytype2=tf_ipsecsha<br />
<br />
http://link.springer.com/referencework/10.1007%2F978-3-642-30194-0<br />
<br />
http://jb.asm.org/content/99/1/326.full.pdf<br />
<br />
=Author=<br />
Urakawa Toshi</div>Bgalindo6106https://microbewiki.kenyon.edu/index.php?title=Moritella_marina&diff=127576Moritella marina2016-12-03T05:22:03Z<p>Bgalindo6106: /* Description and Significance */</p>
<hr />
<div>=Classification=<br />
Domain: Bacteria<br />
<br />
Phylum: Proteobacteria<br />
<br />
Class: Gammaproteobacteria<br />
<br />
Order: Alteromonadales<br />
<br />
Family: Moritellaceae<br />
<br />
Genus: Moritella<br />
<br />
=Species=<br />
''M. japonica, M. yayanosii, M. viscosa, M. profunda, M. abyssi, M. dasanesis''<br />
<br />
<br />
=Description and Significance=<br />
''Moritella marina'' is a gram-negative halophilic psychrophilic facultative anaerobe with curved or straight rods, motile polar flagella that produce PUFA’s (polyunsaturated fatty acids) and DHA (docosahexanoic acid). “Twenty-five years ago, a marine bacterium originally designated ''Vibrio marinus'' and later renamed ''Moritella marina'' MP-1 was reported to produce high levels of DHA (18% of the total fatty acids). Such high levels of DHA presumably provide this bacterium with the ability to maintain appropriate membrane fluidity in the low temperatures and high pressures of its marine environment.”<br />
“Phylogenetic tree of some representatives of the γ-subclass of the class Proteobacteria showing the position of 16S rDNA sequences from some ''Alteromonas macleodii''-related strains and clone sequences from picoplankton DNA from the Mediterranean Sea mesocosm experiment. Sequences of clones 17B161 and 1B161 are available under accession number Y18229 and Y18231, respectively. The tree was calculated by the algorithm of De Soete [16]. The percentage of 500 bootstrap samplings that support branching points above 80% confidence are indicated. The scale bar corresponds to two nucleotide substitutions over 100 sequence positions. The tree was rooted with ''Roseobacter algicola'' as an outgroup.” (FEMS, 1998)<br />
<br />
[[image:Phytree.png|thumbnail|300px| Figure 1: The Family ''Moritellacae''(Urakawa, 2014). ''Moritella viscosa'' is 99% closely related to ''Moritella marina.'' ]]<br />
<br />
=16S Ribosomal RNA Gene Information=<br />
<br />
<br />
[[image:16s.png|thumbnail|300px| Figure 2: Image credit:(Urakawa, 2014)]]<br />
<br />
“Sequence variation at positions 73–98 and 181–214 (E. coli nomenclature) within two variable regions of the 16S rDNA of representatives of clone clusters 1–5 and some strains isolated from mesocosm B. Probe target regions are in bold” (FEMS,1998)<br />
<br />
=Genome Structure=<br />
<br />
Look at Figure: 3<br />
<br />
[[image:GenomeStructure.png|thumbnail|350px| Figure 3: High sequence diversity of Alteromonas macleodii-related cloned and cellular 16S rDNAs from a Mediterranean seawater mesocosm experiment. (FEMS Microbiology Ecology, 2016) ]]<br />
<br />
=Ecology and pathogenesis=<br />
''Moritella marina'' have been found in a variety of cold water marine environments, ranging from the ocean floor to the intestinal tract of marine organisms. Most ''Moritella'' species are thought to live mutualistically amongst larger marine organisms, but ''Moritella viscosa'' has been noted to cause skin ulcers in some fish (Urakawa, 2014). <br />
=Current Research=<br />
''Moritella marina'' has been noted for having unusually high production of the long chain polyunsaturated fatty acids (PUFAs). When ''M. marina'' strain MP-1 was cultured in medium containing cerulenin, a fatty acid synthesis inhibitor, decreases in levels of middle-chain fatty acids and remarkable increases in levels of DHA were observed. These results suggest that the synthesis of middle-chain fatty acids works independently of the synthesis of DHA. M. marina was also found to produce chitonase when induced with chiton (Stefanidi, E., & Vorgias, C. E., 2008). <br />
=References=<br />
<br />
<br />
And, Kumar B. Kautharapu. "Kumar B. Kautharapu." ''Genome Sequence of the Psychrophilic Deep-Sea Bacterium Moritella Marina MP-1'' (ATCC 15381). N.p., 2012. Web. 02 Dec. 2016.<br />
http://jb.asm.org/content/194/22/6296.full<br />
<br />
<br />
Urakawa, H., Kita-Tsukamoto, K., Steven, S. E., Ohwada, K., & Colwell, R. R. (1998). A proposal to transfer Vibrio marinus (Russell 1891) to a new genus Moritella gen. nov. as Moritella marina comb. nov. FEMS microbiology letters, 165(2), 373-378. http://femsle.oxfordjournals.org/content/165/2/373.full#ref-13<br />
<br />
<br />
Pukall, R., Päuker, O., Buntefuß, D., Ulrichs, G., Lebaron, P., Bernard, L., ... & Stackebrandt, E. (1999). High sequence diversity of Alteromonas macleodii-related cloned and cellular 16S rDNAs from a Mediterranean seawater mesocosm experiment. FEMS Microbiology Ecology, 28(4), 335-344.<br />
http://femsec.oxfordjournals.org/content/28/4/335<br />
<br />
<br />
Stefanidi, E., & Vorgias, C. E. (2008). Molecular analysis of the gene encoding a new chitinase from the marine psychrophilic bacterium Moritella marina and biochemical characterization of the recombinant enzyme. Extremophiles, 12(4), 541-552.<br />
"FEMS Microbiology Ecology." High Sequence Diversity of Alteromonas Macleodii-related Cloned and Cellular 16S RDNAs from a Mediterranean Seawater Mesocosm Experiment | FEMS Microbiology Ecology. N.p., n.d. Web. 02 Dec. 2016.<br />
http://femsec.oxfordjournals.org/content/28/4/335 <br />
<br />
<br />
http://www.bacterio.net/moritella.html <br />
<br />
<br />
Genome structure image:<br />
https://www.patricbrc.org/portal/portal/patric/CircosGenomeViewer?cType=genome&cId=1202962.4 <br />
<br />
<br />
http://jb.asm.org/content/194/22/6296.full.pdf<br />
<br />
http://lib.dr.iastate.edu/cgi/viewcontent.cgi?article=1177&context=cbe_pubs<br />
<br />
http://www.biochemsoctrans.org/content/28/6/943.long<br />
<br />
https://www.researchgate.net/publication/232721452_Genome_Sequence_of_the_Psychrophilic_Deep-Sea_Bacterium_Moritella_marina_MP-1_ATCC_15381<br />
<br />
http://aem.asm.org/content/51/4/730.abstract?ijkey=6cfa0071e9fc15e0a007123a571561c86c1354d3&keytype2=tf_ipsecsha<br />
<br />
http://link.springer.com/referencework/10.1007%2F978-3-642-30194-0<br />
<br />
http://jb.asm.org/content/99/1/326.full.pdf <br />
<br />
<br />
=Author=<br />
Urakawa Toshi</div>Bgalindo6106https://microbewiki.kenyon.edu/index.php?title=Moritella_marina&diff=127575Moritella marina2016-12-03T05:21:36Z<p>Bgalindo6106: /* 16S Ribosomal RNA Gene Information */</p>
<hr />
<div>=Classification=<br />
Domain: Bacteria<br />
<br />
Phylum: Proteobacteria<br />
<br />
Class: Gammaproteobacteria<br />
<br />
Order: Alteromonadales<br />
<br />
Family: Moritellaceae<br />
<br />
Genus: Moritella<br />
<br />
=Species=<br />
''M. japonica, M. yayanosii, M. viscosa, M. profunda, M. abyssi, M. dasanesis''<br />
<br />
<br />
=Description and Significance=<br />
''Moritella marina'' is a gram-negative halophilic psychrophilic facultative anaerobe with curved or straight rods, motile polar flagella that produce PUFA’s (polyunsaturated fatty acids) and DHA (docosahexanoic acid). “Twenty-five years ago, a marine bacterium originally designated ''Vibrio marinus'' and later renamed ''Moritella marina'' MP-1 was reported to produce high levels of DHA (18% of the total fatty acids). Such high levels of DHA presumably provide this bacterium with the ability to maintain appropriate membrane fluidity in the low temperatures and high pressures of its marine environment.”<br />
“Phylogenetic tree of some representatives of the γ-subclass of the class Proteobacteria showing the position of 16S rDNA sequences from some ''Alteromonas macleodii''-related strains and clone sequences from picoplankton DNA from the Mediterranean Sea mesocosm experiment. Sequences of clones 17B161 and 1B161 are available under accession number Y18229 and Y18231, respectively. The tree was calculated by the algorithm of De Soete [16]. The percentage of 500 bootstrap samplings that support branching points above 80% confidence are indicated. The scale bar corresponds to two nucleotide substitutions over 100 sequence positions. The tree was rooted with ''Roseobacter algicola'' as an outgroup.” (FEMS, 1998)<br />
<br />
[[image:Phytree.png|thumbnail|300px| Figure 1: The Family ''Moritellacae''(Toshi, 2014). ''Moritella viscosa'' is 99% closely related to ''Moritella marina.'' ]]<br />
<br />
=16S Ribosomal RNA Gene Information=<br />
<br />
<br />
[[image:16s.png|thumbnail|300px| Figure 2: Image credit:(Urakawa, 2014)]]<br />
<br />
“Sequence variation at positions 73–98 and 181–214 (E. coli nomenclature) within two variable regions of the 16S rDNA of representatives of clone clusters 1–5 and some strains isolated from mesocosm B. Probe target regions are in bold” (FEMS,1998)<br />
<br />
=Genome Structure=<br />
<br />
Look at Figure: 3<br />
<br />
[[image:GenomeStructure.png|thumbnail|350px| Figure 3: High sequence diversity of Alteromonas macleodii-related cloned and cellular 16S rDNAs from a Mediterranean seawater mesocosm experiment. (FEMS Microbiology Ecology, 2016) ]]<br />
<br />
=Ecology and pathogenesis=<br />
''Moritella marina'' have been found in a variety of cold water marine environments, ranging from the ocean floor to the intestinal tract of marine organisms. Most ''Moritella'' species are thought to live mutualistically amongst larger marine organisms, but ''Moritella viscosa'' has been noted to cause skin ulcers in some fish (Urakawa, 2014). <br />
=Current Research=<br />
''Moritella marina'' has been noted for having unusually high production of the long chain polyunsaturated fatty acids (PUFAs). When ''M. marina'' strain MP-1 was cultured in medium containing cerulenin, a fatty acid synthesis inhibitor, decreases in levels of middle-chain fatty acids and remarkable increases in levels of DHA were observed. These results suggest that the synthesis of middle-chain fatty acids works independently of the synthesis of DHA. M. marina was also found to produce chitonase when induced with chiton (Stefanidi, E., & Vorgias, C. E., 2008). <br />
=References=<br />
<br />
<br />
And, Kumar B. Kautharapu. "Kumar B. Kautharapu." ''Genome Sequence of the Psychrophilic Deep-Sea Bacterium Moritella Marina MP-1'' (ATCC 15381). N.p., 2012. Web. 02 Dec. 2016.<br />
http://jb.asm.org/content/194/22/6296.full<br />
<br />
<br />
Urakawa, H., Kita-Tsukamoto, K., Steven, S. E., Ohwada, K., & Colwell, R. R. (1998). A proposal to transfer Vibrio marinus (Russell 1891) to a new genus Moritella gen. nov. as Moritella marina comb. nov. FEMS microbiology letters, 165(2), 373-378. http://femsle.oxfordjournals.org/content/165/2/373.full#ref-13<br />
<br />
<br />
Pukall, R., Päuker, O., Buntefuß, D., Ulrichs, G., Lebaron, P., Bernard, L., ... & Stackebrandt, E. (1999). High sequence diversity of Alteromonas macleodii-related cloned and cellular 16S rDNAs from a Mediterranean seawater mesocosm experiment. FEMS Microbiology Ecology, 28(4), 335-344.<br />
http://femsec.oxfordjournals.org/content/28/4/335<br />
<br />
<br />
Stefanidi, E., & Vorgias, C. E. (2008). Molecular analysis of the gene encoding a new chitinase from the marine psychrophilic bacterium Moritella marina and biochemical characterization of the recombinant enzyme. Extremophiles, 12(4), 541-552.<br />
"FEMS Microbiology Ecology." High Sequence Diversity of Alteromonas Macleodii-related Cloned and Cellular 16S RDNAs from a Mediterranean Seawater Mesocosm Experiment | FEMS Microbiology Ecology. N.p., n.d. Web. 02 Dec. 2016.<br />
http://femsec.oxfordjournals.org/content/28/4/335 <br />
<br />
<br />
http://www.bacterio.net/moritella.html <br />
<br />
<br />
Genome structure image:<br />
https://www.patricbrc.org/portal/portal/patric/CircosGenomeViewer?cType=genome&cId=1202962.4 <br />
<br />
<br />
http://jb.asm.org/content/194/22/6296.full.pdf<br />
<br />
http://lib.dr.iastate.edu/cgi/viewcontent.cgi?article=1177&context=cbe_pubs<br />
<br />
http://www.biochemsoctrans.org/content/28/6/943.long<br />
<br />
https://www.researchgate.net/publication/232721452_Genome_Sequence_of_the_Psychrophilic_Deep-Sea_Bacterium_Moritella_marina_MP-1_ATCC_15381<br />
<br />
http://aem.asm.org/content/51/4/730.abstract?ijkey=6cfa0071e9fc15e0a007123a571561c86c1354d3&keytype2=tf_ipsecsha<br />
<br />
http://link.springer.com/referencework/10.1007%2F978-3-642-30194-0<br />
<br />
http://jb.asm.org/content/99/1/326.full.pdf <br />
<br />
<br />
=Author=<br />
Urakawa Toshi</div>Bgalindo6106https://microbewiki.kenyon.edu/index.php?title=Moritella_marina&diff=127574Moritella marina2016-12-03T05:18:41Z<p>Bgalindo6106: /* Genome Structure */</p>
<hr />
<div>=Classification=<br />
Domain: Bacteria<br />
<br />
Phylum: Proteobacteria<br />
<br />
Class: Gammaproteobacteria<br />
<br />
Order: Alteromonadales<br />
<br />
Family: Moritellaceae<br />
<br />
Genus: Moritella<br />
<br />
=Species=<br />
''M. japonica, M. yayanosii, M. viscosa, M. profunda, M. abyssi, M. dasanesis''<br />
<br />
<br />
=Description and Significance=<br />
''Moritella marina'' is a gram-negative halophilic psychrophilic facultative anaerobe with curved or straight rods, motile polar flagella that produce PUFA’s (polyunsaturated fatty acids) and DHA (docosahexanoic acid). “Twenty-five years ago, a marine bacterium originally designated ''Vibrio marinus'' and later renamed ''Moritella marina'' MP-1 was reported to produce high levels of DHA (18% of the total fatty acids). Such high levels of DHA presumably provide this bacterium with the ability to maintain appropriate membrane fluidity in the low temperatures and high pressures of its marine environment.”<br />
“Phylogenetic tree of some representatives of the γ-subclass of the class Proteobacteria showing the position of 16S rDNA sequences from some ''Alteromonas macleodii''-related strains and clone sequences from picoplankton DNA from the Mediterranean Sea mesocosm experiment. Sequences of clones 17B161 and 1B161 are available under accession number Y18229 and Y18231, respectively. The tree was calculated by the algorithm of De Soete [16]. The percentage of 500 bootstrap samplings that support branching points above 80% confidence are indicated. The scale bar corresponds to two nucleotide substitutions over 100 sequence positions. The tree was rooted with ''Roseobacter algicola'' as an outgroup.” (FEMS, 1998)<br />
<br />
[[image:Phytree.png|thumbnail|300px| Figure 1: The Family ''Moritellacae''(Toshi, 2014). ''Moritella viscosa'' is 99% closely related to ''Moritella marina.'' ]]<br />
<br />
=16S Ribosomal RNA Gene Information=<br />
<br />
<br />
[[image:16s.png|thumbnail|300px| Figure 2: Image credit:(Toshi, 2014)]]<br />
<br />
“Sequence variation at positions 73–98 and 181–214 (E. coli nomenclature) within two variable regions of the 16S rDNA of representatives of clone clusters 1–5 and some strains isolated from mesocosm B. Probe target regions are in bold” (FEMS,1998)<br />
<br />
=Genome Structure=<br />
<br />
Look at Figure: 3<br />
<br />
[[image:GenomeStructure.png|thumbnail|350px| Figure 3: High sequence diversity of Alteromonas macleodii-related cloned and cellular 16S rDNAs from a Mediterranean seawater mesocosm experiment. (FEMS Microbiology Ecology, 2016) ]]<br />
<br />
=Ecology and pathogenesis=<br />
''Moritella marina'' have been found in a variety of cold water marine environments, ranging from the ocean floor to the intestinal tract of marine organisms. Most ''Moritella'' species are thought to live mutualistically amongst larger marine organisms, but ''Moritella viscosa'' has been noted to cause skin ulcers in some fish (Urakawa, 2014). <br />
=Current Research=<br />
''Moritella marina'' has been noted for having unusually high production of the long chain polyunsaturated fatty acids (PUFAs). When ''M. marina'' strain MP-1 was cultured in medium containing cerulenin, a fatty acid synthesis inhibitor, decreases in levels of middle-chain fatty acids and remarkable increases in levels of DHA were observed. These results suggest that the synthesis of middle-chain fatty acids works independently of the synthesis of DHA. M. marina was also found to produce chitonase when induced with chiton (Stefanidi, E., & Vorgias, C. E., 2008). <br />
=References=<br />
<br />
<br />
And, Kumar B. Kautharapu. "Kumar B. Kautharapu." ''Genome Sequence of the Psychrophilic Deep-Sea Bacterium Moritella Marina MP-1'' (ATCC 15381). N.p., 2012. Web. 02 Dec. 2016.<br />
http://jb.asm.org/content/194/22/6296.full<br />
<br />
<br />
Urakawa, H., Kita-Tsukamoto, K., Steven, S. E., Ohwada, K., & Colwell, R. R. (1998). A proposal to transfer Vibrio marinus (Russell 1891) to a new genus Moritella gen. nov. as Moritella marina comb. nov. FEMS microbiology letters, 165(2), 373-378. http://femsle.oxfordjournals.org/content/165/2/373.full#ref-13<br />
<br />
<br />
Pukall, R., Päuker, O., Buntefuß, D., Ulrichs, G., Lebaron, P., Bernard, L., ... & Stackebrandt, E. (1999). High sequence diversity of Alteromonas macleodii-related cloned and cellular 16S rDNAs from a Mediterranean seawater mesocosm experiment. FEMS Microbiology Ecology, 28(4), 335-344.<br />
http://femsec.oxfordjournals.org/content/28/4/335<br />
<br />
<br />
Stefanidi, E., & Vorgias, C. E. (2008). Molecular analysis of the gene encoding a new chitinase from the marine psychrophilic bacterium Moritella marina and biochemical characterization of the recombinant enzyme. Extremophiles, 12(4), 541-552.<br />
"FEMS Microbiology Ecology." High Sequence Diversity of Alteromonas Macleodii-related Cloned and Cellular 16S RDNAs from a Mediterranean Seawater Mesocosm Experiment | FEMS Microbiology Ecology. N.p., n.d. Web. 02 Dec. 2016.<br />
http://femsec.oxfordjournals.org/content/28/4/335 <br />
<br />
<br />
http://www.bacterio.net/moritella.html <br />
<br />
<br />
Genome structure image:<br />
https://www.patricbrc.org/portal/portal/patric/CircosGenomeViewer?cType=genome&cId=1202962.4 <br />
<br />
<br />
http://jb.asm.org/content/194/22/6296.full.pdf<br />
<br />
http://lib.dr.iastate.edu/cgi/viewcontent.cgi?article=1177&context=cbe_pubs<br />
<br />
http://www.biochemsoctrans.org/content/28/6/943.long<br />
<br />
https://www.researchgate.net/publication/232721452_Genome_Sequence_of_the_Psychrophilic_Deep-Sea_Bacterium_Moritella_marina_MP-1_ATCC_15381<br />
<br />
http://aem.asm.org/content/51/4/730.abstract?ijkey=6cfa0071e9fc15e0a007123a571561c86c1354d3&keytype2=tf_ipsecsha<br />
<br />
http://link.springer.com/referencework/10.1007%2F978-3-642-30194-0<br />
<br />
http://jb.asm.org/content/99/1/326.full.pdf <br />
<br />
<br />
=Author=<br />
Urakawa Toshi</div>Bgalindo6106https://microbewiki.kenyon.edu/index.php?title=Moritella_marina&diff=127573Moritella marina2016-12-03T05:17:13Z<p>Bgalindo6106: /* Classification */</p>
<hr />
<div>=Classification=<br />
Domain: Bacteria<br />
<br />
Phylum: Proteobacteria<br />
<br />
Class: Gammaproteobacteria<br />
<br />
Order: Alteromonadales<br />
<br />
Family: Moritellaceae<br />
<br />
Genus: Moritella<br />
<br />
=Species=<br />
''M. japonica, M. yayanosii, M. viscosa, M. profunda, M. abyssi, M. dasanesis''<br />
<br />
<br />
=Description and Significance=<br />
''Moritella marina'' is a gram-negative halophilic psychrophilic facultative anaerobe with curved or straight rods, motile polar flagella that produce PUFA’s (polyunsaturated fatty acids) and DHA (docosahexanoic acid). “Twenty-five years ago, a marine bacterium originally designated ''Vibrio marinus'' and later renamed ''Moritella marina'' MP-1 was reported to produce high levels of DHA (18% of the total fatty acids). Such high levels of DHA presumably provide this bacterium with the ability to maintain appropriate membrane fluidity in the low temperatures and high pressures of its marine environment.”<br />
“Phylogenetic tree of some representatives of the γ-subclass of the class Proteobacteria showing the position of 16S rDNA sequences from some ''Alteromonas macleodii''-related strains and clone sequences from picoplankton DNA from the Mediterranean Sea mesocosm experiment. Sequences of clones 17B161 and 1B161 are available under accession number Y18229 and Y18231, respectively. The tree was calculated by the algorithm of De Soete [16]. The percentage of 500 bootstrap samplings that support branching points above 80% confidence are indicated. The scale bar corresponds to two nucleotide substitutions over 100 sequence positions. The tree was rooted with ''Roseobacter algicola'' as an outgroup.” (FEMS, 1998)<br />
<br />
[[image:Phytree.png|thumbnail|300px| Figure 1: The Family ''Moritellacae''(Toshi, 2014). ''Moritella viscosa'' is 99% closely related to ''Moritella marina.'' ]]<br />
<br />
=16S Ribosomal RNA Gene Information=<br />
<br />
<br />
[[image:16s.png|thumbnail|300px| Figure 2: Image credit:(Toshi, 2014)]]<br />
<br />
“Sequence variation at positions 73–98 and 181–214 (E. coli nomenclature) within two variable regions of the 16S rDNA of representatives of clone clusters 1–5 and some strains isolated from mesocosm B. Probe target regions are in bold” (FEMS,1998)<br />
<br />
=Genome Structure=<br />
<br />
Image 3<br />
<br />
[[image:GenomeStructure.png|thumbnail|350px| Figure 3: High sequence diversity of Alteromonas macleodii-related cloned and cellular 16S rDNAs from a Mediterranean seawater mesocosm experiment. (FEMS Microbiology Ecology, 2016) ]]<br />
<br />
=Ecology and pathogenesis=<br />
''Moritella marina'' have been found in a variety of cold water marine environments, ranging from the ocean floor to the intestinal tract of marine organisms. Most ''Moritella'' species are thought to live mutualistically amongst larger marine organisms, but ''Moritella viscosa'' has been noted to cause skin ulcers in some fish (Urakawa, 2014). <br />
=Current Research=<br />
''Moritella marina'' has been noted for having unusually high production of the long chain polyunsaturated fatty acids (PUFAs). When ''M. marina'' strain MP-1 was cultured in medium containing cerulenin, a fatty acid synthesis inhibitor, decreases in levels of middle-chain fatty acids and remarkable increases in levels of DHA were observed. These results suggest that the synthesis of middle-chain fatty acids works independently of the synthesis of DHA. M. marina was also found to produce chitonase when induced with chiton (Stefanidi, E., & Vorgias, C. E., 2008). <br />
=References=<br />
<br />
<br />
And, Kumar B. Kautharapu. "Kumar B. Kautharapu." ''Genome Sequence of the Psychrophilic Deep-Sea Bacterium Moritella Marina MP-1'' (ATCC 15381). N.p., 2012. Web. 02 Dec. 2016.<br />
http://jb.asm.org/content/194/22/6296.full<br />
<br />
<br />
Urakawa, H., Kita-Tsukamoto, K., Steven, S. E., Ohwada, K., & Colwell, R. R. (1998). A proposal to transfer Vibrio marinus (Russell 1891) to a new genus Moritella gen. nov. as Moritella marina comb. nov. FEMS microbiology letters, 165(2), 373-378. http://femsle.oxfordjournals.org/content/165/2/373.full#ref-13<br />
<br />
<br />
Pukall, R., Päuker, O., Buntefuß, D., Ulrichs, G., Lebaron, P., Bernard, L., ... & Stackebrandt, E. (1999). High sequence diversity of Alteromonas macleodii-related cloned and cellular 16S rDNAs from a Mediterranean seawater mesocosm experiment. FEMS Microbiology Ecology, 28(4), 335-344.<br />
http://femsec.oxfordjournals.org/content/28/4/335<br />
<br />
<br />
Stefanidi, E., & Vorgias, C. E. (2008). Molecular analysis of the gene encoding a new chitinase from the marine psychrophilic bacterium Moritella marina and biochemical characterization of the recombinant enzyme. Extremophiles, 12(4), 541-552.<br />
"FEMS Microbiology Ecology." High Sequence Diversity of Alteromonas Macleodii-related Cloned and Cellular 16S RDNAs from a Mediterranean Seawater Mesocosm Experiment | FEMS Microbiology Ecology. N.p., n.d. Web. 02 Dec. 2016.<br />
http://femsec.oxfordjournals.org/content/28/4/335 <br />
<br />
<br />
http://www.bacterio.net/moritella.html <br />
<br />
<br />
Genome structure image:<br />
https://www.patricbrc.org/portal/portal/patric/CircosGenomeViewer?cType=genome&cId=1202962.4 <br />
<br />
<br />
http://jb.asm.org/content/194/22/6296.full.pdf<br />
<br />
http://lib.dr.iastate.edu/cgi/viewcontent.cgi?article=1177&context=cbe_pubs<br />
<br />
http://www.biochemsoctrans.org/content/28/6/943.long<br />
<br />
https://www.researchgate.net/publication/232721452_Genome_Sequence_of_the_Psychrophilic_Deep-Sea_Bacterium_Moritella_marina_MP-1_ATCC_15381<br />
<br />
http://aem.asm.org/content/51/4/730.abstract?ijkey=6cfa0071e9fc15e0a007123a571561c86c1354d3&keytype2=tf_ipsecsha<br />
<br />
http://link.springer.com/referencework/10.1007%2F978-3-642-30194-0<br />
<br />
http://jb.asm.org/content/99/1/326.full.pdf <br />
<br />
<br />
=Author=<br />
Urakawa Toshi</div>Bgalindo6106https://microbewiki.kenyon.edu/index.php?title=Moritella_marina&diff=127572Moritella marina2016-12-03T05:15:24Z<p>Bgalindo6106: </p>
<hr />
<div>=Classification=<br />
Domain: Bacteria<br />
Phylum: Proteobacteria<br />
Class: Gammaproteobacteria<br />
Order: Alteromonadales<br />
Family: Moritellaceae<br />
Genus: Moritella<br />
<br />
<br />
=Species=<br />
''M. japonica, M. yayanosii, M. viscosa, M. profunda, M. abyssi, M. dasanesis''<br />
<br />
<br />
=Description and Significance=<br />
''Moritella marina'' is a gram-negative halophilic psychrophilic facultative anaerobe with curved or straight rods, motile polar flagella that produce PUFA’s (polyunsaturated fatty acids) and DHA (docosahexanoic acid). “Twenty-five years ago, a marine bacterium originally designated ''Vibrio marinus'' and later renamed ''Moritella marina'' MP-1 was reported to produce high levels of DHA (18% of the total fatty acids). Such high levels of DHA presumably provide this bacterium with the ability to maintain appropriate membrane fluidity in the low temperatures and high pressures of its marine environment.”<br />
“Phylogenetic tree of some representatives of the γ-subclass of the class Proteobacteria showing the position of 16S rDNA sequences from some ''Alteromonas macleodii''-related strains and clone sequences from picoplankton DNA from the Mediterranean Sea mesocosm experiment. Sequences of clones 17B161 and 1B161 are available under accession number Y18229 and Y18231, respectively. The tree was calculated by the algorithm of De Soete [16]. The percentage of 500 bootstrap samplings that support branching points above 80% confidence are indicated. The scale bar corresponds to two nucleotide substitutions over 100 sequence positions. The tree was rooted with ''Roseobacter algicola'' as an outgroup.” (FEMS, 1998)<br />
<br />
[[image:Phytree.png|thumbnail|300px| Figure 1: The Family ''Moritellacae''(Toshi, 2014). ''Moritella viscosa'' is 99% closely related to ''Moritella marina.'' ]]<br />
<br />
=16S Ribosomal RNA Gene Information=<br />
<br />
<br />
[[image:16s.png|thumbnail|300px| Figure 2: Image credit:(Toshi, 2014)]]<br />
<br />
“Sequence variation at positions 73–98 and 181–214 (E. coli nomenclature) within two variable regions of the 16S rDNA of representatives of clone clusters 1–5 and some strains isolated from mesocosm B. Probe target regions are in bold” (FEMS,1998)<br />
<br />
=Genome Structure=<br />
<br />
Image 3<br />
<br />
[[image:GenomeStructure.png|thumbnail|350px| Figure 3: High sequence diversity of Alteromonas macleodii-related cloned and cellular 16S rDNAs from a Mediterranean seawater mesocosm experiment. (FEMS Microbiology Ecology, 2016) ]]<br />
<br />
=Ecology and pathogenesis=<br />
''Moritella marina'' have been found in a variety of cold water marine environments, ranging from the ocean floor to the intestinal tract of marine organisms. Most ''Moritella'' species are thought to live mutualistically amongst larger marine organisms, but ''Moritella viscosa'' has been noted to cause skin ulcers in some fish (Urakawa, 2014). <br />
=Current Research=<br />
''Moritella marina'' has been noted for having unusually high production of the long chain polyunsaturated fatty acids (PUFAs). When ''M. marina'' strain MP-1 was cultured in medium containing cerulenin, a fatty acid synthesis inhibitor, decreases in levels of middle-chain fatty acids and remarkable increases in levels of DHA were observed. These results suggest that the synthesis of middle-chain fatty acids works independently of the synthesis of DHA. M. marina was also found to produce chitonase when induced with chiton (Stefanidi, E., & Vorgias, C. E., 2008). <br />
=References=<br />
<br />
<br />
And, Kumar B. Kautharapu. "Kumar B. Kautharapu." ''Genome Sequence of the Psychrophilic Deep-Sea Bacterium Moritella Marina MP-1'' (ATCC 15381). N.p., 2012. Web. 02 Dec. 2016.<br />
http://jb.asm.org/content/194/22/6296.full<br />
<br />
<br />
Urakawa, H., Kita-Tsukamoto, K., Steven, S. E., Ohwada, K., & Colwell, R. R. (1998). A proposal to transfer Vibrio marinus (Russell 1891) to a new genus Moritella gen. nov. as Moritella marina comb. nov. FEMS microbiology letters, 165(2), 373-378. http://femsle.oxfordjournals.org/content/165/2/373.full#ref-13<br />
<br />
<br />
Pukall, R., Päuker, O., Buntefuß, D., Ulrichs, G., Lebaron, P., Bernard, L., ... & Stackebrandt, E. (1999). High sequence diversity of Alteromonas macleodii-related cloned and cellular 16S rDNAs from a Mediterranean seawater mesocosm experiment. FEMS Microbiology Ecology, 28(4), 335-344.<br />
http://femsec.oxfordjournals.org/content/28/4/335<br />
<br />
<br />
Stefanidi, E., & Vorgias, C. E. (2008). Molecular analysis of the gene encoding a new chitinase from the marine psychrophilic bacterium Moritella marina and biochemical characterization of the recombinant enzyme. Extremophiles, 12(4), 541-552.<br />
"FEMS Microbiology Ecology." High Sequence Diversity of Alteromonas Macleodii-related Cloned and Cellular 16S RDNAs from a Mediterranean Seawater Mesocosm Experiment | FEMS Microbiology Ecology. N.p., n.d. Web. 02 Dec. 2016.<br />
http://femsec.oxfordjournals.org/content/28/4/335 <br />
<br />
<br />
http://www.bacterio.net/moritella.html <br />
<br />
<br />
Genome structure image:<br />
https://www.patricbrc.org/portal/portal/patric/CircosGenomeViewer?cType=genome&cId=1202962.4 <br />
<br />
<br />
http://jb.asm.org/content/194/22/6296.full.pdf<br />
<br />
http://lib.dr.iastate.edu/cgi/viewcontent.cgi?article=1177&context=cbe_pubs<br />
<br />
http://www.biochemsoctrans.org/content/28/6/943.long<br />
<br />
https://www.researchgate.net/publication/232721452_Genome_Sequence_of_the_Psychrophilic_Deep-Sea_Bacterium_Moritella_marina_MP-1_ATCC_15381<br />
<br />
http://aem.asm.org/content/51/4/730.abstract?ijkey=6cfa0071e9fc15e0a007123a571561c86c1354d3&keytype2=tf_ipsecsha<br />
<br />
http://link.springer.com/referencework/10.1007%2F978-3-642-30194-0<br />
<br />
http://jb.asm.org/content/99/1/326.full.pdf <br />
<br />
<br />
=Author=<br />
Urakawa Toshi</div>Bgalindo6106https://microbewiki.kenyon.edu/index.php?title=Moritella_marina&diff=127567Moritella marina2016-12-02T21:54:40Z<p>Bgalindo6106: /* Genome Structure */</p>
<hr />
<div>=Classification=<br />
Domain: Bacteria<br />
Phylum: Proteobacteria<br />
Class: Gammaproteobacteria<br />
Order: Alteromonadales<br />
Family: Moritellaceae<br />
Genus: Moritella<br />
<br />
<br />
=Species=<br />
''M. japonica, M. yayanosii, M. viscosa, M. profunda, M. abyssi, M. dasanesis''<br />
<br />
<br />
=Description and Significance=<br />
''Moritella marina'' is a gram-negative halophilic psychrophilic facultative anaerobe with curved or straight rods, motile polar flagella that produce PUFA’s (polyunsaturated fatty acids) and DHA (docosahexanoic acid). “Twenty-five years ago, a marine bacterium originally designated ''Vibrio marinus'' and later renamed ''Moritella marina'' MP-1 was reported to produce high levels of DHA (18% of the total fatty acids). Such high levels of DHA presumably provide this bacterium with the ability to maintain appropriate membrane fluidity in the low temperatures and high pressures of its marine environment.”<br />
“Phylogenetic tree of some representatives of the γ-subclass of the class Proteobacteria showing the position of 16S rDNA sequences from some ''Alteromonas macleodii''-related strains and clone sequences from picoplankton DNA from the Mediterranean Sea mesocosm experiment. Sequences of clones 17B161 and 1B161 are available under accession number Y18229 and Y18231, respectively. The tree was calculated by the algorithm of De Soete [16]. The percentage of 500 bootstrap samplings that support branching points above 80% confidence are indicated. The scale bar corresponds to two nucleotide substitutions over 100 sequence positions. The tree was rooted with ''Roseobacter algicola'' as an outgroup.” (FEMS, 1998)<br />
<br />
[[image:Phytree.png|thumbnail|300px| Figure 1: The Family ''Moritellacae''(Toshi, 2014). ''Moritella viscosa'' is 99% closely related to ''Moritella marina.'' ]]<br />
<br />
=16S Ribosomal RNA Gene Information=<br />
<br />
<br />
[[image:16s.png|thumbnail|300px| Figure 2: Image credit:(Toshi, 2014)]]<br />
<br />
“Sequence variation at positions 73–98 and 181–214 (E. coli nomenclature) within two variable regions of the 16S rDNA of representatives of clone clusters 1–5 and some strains isolated from mesocosm B. Probe target regions are in bold” (FEMS,1998)<br />
<br />
=Genome Structure=<br />
<br />
Image 3<br />
<br />
[[image:GenomeStructure.png|thumbnail|350px| Figure 3: High sequence diversity of Alteromonas macleodii-related cloned and cellular 16S rDNAs from a Mediterranean seawater mesocosm experiment. (FEMS Microbiology Ecology, 2016) ]]<br />
<br />
=Ecology and pathogenesis=<br />
''Moritella marina'' have been found in a variety of cold water marine environments, ranging from the ocean floor to the intestinal tract of marine organisms. Most ''Moritella'' species are thought to live mutualistically amongst larger marine organisms, but ''Moritella viscosa'' has been noted to cause skin ulcers in some fish (Urakawa, 2014). <br />
=Current Research=<br />
''Moritella marina'' has been noted for having unusually high production of the long chain polyunsaturated fatty acids (PUFAs). When ''M. marina'' strain MP-1 was cultured in medium containing cerulenin, a fatty acid synthesis inhibitor, decreases in levels of middle-chain fatty acids and remarkable increases in levels of DHA were observed. These results suggest that the synthesis of middle-chain fatty acids works independently of the synthesis of DHA. M. marina was also found to produce chitonase when induced with chiton (Stefanidi, E., & Vorgias, C. E., 2008). <br />
=References=<br />
<br />
<br />
And, Kumar B. Kautharapu. "Kumar B. Kautharapu." ''Genome Sequence of the Psychrophilic Deep-Sea Bacterium Moritella Marina MP-1'' (ATCC 15381). N.p., 2012. Web. 02 Dec. 2016.<br />
http://jb.asm.org/content/194/22/6296.full<br />
<br />
<br />
Urakawa, H., Kita-Tsukamoto, K., Steven, S. E., Ohwada, K., & Colwell, R. R. (1998). A proposal to transfer Vibrio marinus (Russell 1891) to a new genus Moritella gen. nov. as Moritella marina comb. nov. FEMS microbiology letters, 165(2), 373-378. http://femsle.oxfordjournals.org/content/165/2/373.full#ref-13<br />
<br />
<br />
Pukall, R., Päuker, O., Buntefuß, D., Ulrichs, G., Lebaron, P., Bernard, L., ... & Stackebrandt, E. (1999). High sequence diversity of Alteromonas macleodii-related cloned and cellular 16S rDNAs from a Mediterranean seawater mesocosm experiment. FEMS Microbiology Ecology, 28(4), 335-344.<br />
http://femsec.oxfordjournals.org/content/28/4/335<br />
<br />
<br />
Stefanidi, E., & Vorgias, C. E. (2008). Molecular analysis of the gene encoding a new chitinase from the marine psychrophilic bacterium Moritella marina and biochemical characterization of the recombinant enzyme. Extremophiles, 12(4), 541-552.<br />
"FEMS Microbiology Ecology." High Sequence Diversity of Alteromonas Macleodii-related Cloned and Cellular 16S RDNAs from a Mediterranean Seawater Mesocosm Experiment | FEMS Microbiology Ecology. N.p., n.d. Web. 02 Dec. 2016.<br />
http://femsec.oxfordjournals.org/content/28/4/335 <br />
<br />
<br />
http://www.bacterio.net/moritella.html <br />
<br />
<br />
Genome structure image:<br />
https://www.patricbrc.org/portal/portal/patric/CircosGenomeViewer?cType=genome&cId=1202962.4 <br />
<br />
<br />
http://jb.asm.org/content/194/22/6296.full.pdf<br />
<br />
http://lib.dr.iastate.edu/cgi/viewcontent.cgi?article=1177&context=cbe_pubs<br />
<br />
http://www.biochemsoctrans.org/content/28/6/943.long<br />
<br />
https://www.researchgate.net/publication/232721452_Genome_Sequence_of_the_Psychrophilic_Deep-Sea_Bacterium_Moritella_marina_MP-1_ATCC_15381<br />
<br />
http://aem.asm.org/content/51/4/730.abstract?ijkey=6cfa0071e9fc15e0a007123a571561c86c1354d3&keytype2=tf_ipsecsha<br />
<br />
http://link.springer.com/referencework/10.1007%2F978-3-642-30194-0<br />
<br />
http://jb.asm.org/content/99/1/326.full.pdf <br />
<br />
<br />
=Author=<br />
Dr. Toshi</div>Bgalindo6106https://microbewiki.kenyon.edu/index.php?title=Moritella_marina&diff=127566Moritella marina2016-12-02T21:51:26Z<p>Bgalindo6106: </p>
<hr />
<div>=Classification=<br />
Domain: Bacteria<br />
Phylum: Proteobacteria<br />
Class: Gammaproteobacteria<br />
Order: Alteromonadales<br />
Family: Moritellaceae<br />
Genus: Moritella<br />
<br />
<br />
=Species=<br />
''M. japonica, M. yayanosii, M. viscosa, M. profunda, M. abyssi, M. dasanesis''<br />
<br />
<br />
=Description and Significance=<br />
''Moritella marina'' is a gram-negative halophilic psychrophilic facultative anaerobe with curved or straight rods, motile polar flagella that produce PUFA’s (polyunsaturated fatty acids) and DHA (docosahexanoic acid). “Twenty-five years ago, a marine bacterium originally designated ''Vibrio marinus'' and later renamed ''Moritella marina'' MP-1 was reported to produce high levels of DHA (18% of the total fatty acids). Such high levels of DHA presumably provide this bacterium with the ability to maintain appropriate membrane fluidity in the low temperatures and high pressures of its marine environment.”<br />
“Phylogenetic tree of some representatives of the γ-subclass of the class Proteobacteria showing the position of 16S rDNA sequences from some ''Alteromonas macleodii''-related strains and clone sequences from picoplankton DNA from the Mediterranean Sea mesocosm experiment. Sequences of clones 17B161 and 1B161 are available under accession number Y18229 and Y18231, respectively. The tree was calculated by the algorithm of De Soete [16]. The percentage of 500 bootstrap samplings that support branching points above 80% confidence are indicated. The scale bar corresponds to two nucleotide substitutions over 100 sequence positions. The tree was rooted with ''Roseobacter algicola'' as an outgroup.” (FEMS, 1998)<br />
<br />
[[image:Phytree.png|thumbnail|300px| Figure 1: The Family ''Moritellacae''(Toshi, 2014). ''Moritella viscosa'' is 99% closely related to ''Moritella marina.'' ]]<br />
<br />
=16S Ribosomal RNA Gene Information=<br />
<br />
<br />
[[image:16s.png|thumbnail|300px| Figure 2: Image credit:(Toshi, 2014)]]<br />
<br />
“Sequence variation at positions 73–98 and 181–214 (E. coli nomenclature) within two variable regions of the 16S rDNA of representatives of clone clusters 1–5 and some strains isolated from mesocosm B. Probe target regions are in bold” (FEMS,1998)<br />
<br />
=Genome Structure=<br />
[[image:GenomeStructure.png|thumbnail|350px| Figure 2:.]]<br />
<br />
<br />
=Ecology and pathogenesis=<br />
''Moritella marina'' have been found in a variety of cold water marine environments, ranging from the ocean floor to the intestinal tract of marine organisms. Most ''Moritella'' species are thought to live mutualistically amongst larger marine organisms, but ''Moritella viscosa'' has been noted to cause skin ulcers in some fish (Urakawa, 2014). <br />
=Current Research=<br />
''Moritella marina'' has been noted for having unusually high production of the long chain polyunsaturated fatty acids (PUFAs). When ''M. marina'' strain MP-1 was cultured in medium containing cerulenin, a fatty acid synthesis inhibitor, decreases in levels of middle-chain fatty acids and remarkable increases in levels of DHA were observed. These results suggest that the synthesis of middle-chain fatty acids works independently of the synthesis of DHA. M. marina was also found to produce chitonase when induced with chiton (Stefanidi, E., & Vorgias, C. E., 2008). <br />
=References=<br />
<br />
<br />
And, Kumar B. Kautharapu. "Kumar B. Kautharapu." ''Genome Sequence of the Psychrophilic Deep-Sea Bacterium Moritella Marina MP-1'' (ATCC 15381). N.p., 2012. Web. 02 Dec. 2016.<br />
http://jb.asm.org/content/194/22/6296.full<br />
<br />
<br />
Urakawa, H., Kita-Tsukamoto, K., Steven, S. E., Ohwada, K., & Colwell, R. R. (1998). A proposal to transfer Vibrio marinus (Russell 1891) to a new genus Moritella gen. nov. as Moritella marina comb. nov. FEMS microbiology letters, 165(2), 373-378. http://femsle.oxfordjournals.org/content/165/2/373.full#ref-13<br />
<br />
<br />
Pukall, R., Päuker, O., Buntefuß, D., Ulrichs, G., Lebaron, P., Bernard, L., ... & Stackebrandt, E. (1999). High sequence diversity of Alteromonas macleodii-related cloned and cellular 16S rDNAs from a Mediterranean seawater mesocosm experiment. FEMS Microbiology Ecology, 28(4), 335-344.<br />
http://femsec.oxfordjournals.org/content/28/4/335<br />
<br />
<br />
Stefanidi, E., & Vorgias, C. E. (2008). Molecular analysis of the gene encoding a new chitinase from the marine psychrophilic bacterium Moritella marina and biochemical characterization of the recombinant enzyme. Extremophiles, 12(4), 541-552.<br />
"FEMS Microbiology Ecology." High Sequence Diversity of Alteromonas Macleodii-related Cloned and Cellular 16S RDNAs from a Mediterranean Seawater Mesocosm Experiment | FEMS Microbiology Ecology. N.p., n.d. Web. 02 Dec. 2016.<br />
http://femsec.oxfordjournals.org/content/28/4/335 <br />
<br />
<br />
http://www.bacterio.net/moritella.html <br />
<br />
<br />
Genome structure image:<br />
https://www.patricbrc.org/portal/portal/patric/CircosGenomeViewer?cType=genome&cId=1202962.4 <br />
<br />
<br />
http://jb.asm.org/content/194/22/6296.full.pdf<br />
<br />
http://lib.dr.iastate.edu/cgi/viewcontent.cgi?article=1177&context=cbe_pubs<br />
<br />
http://www.biochemsoctrans.org/content/28/6/943.long<br />
<br />
https://www.researchgate.net/publication/232721452_Genome_Sequence_of_the_Psychrophilic_Deep-Sea_Bacterium_Moritella_marina_MP-1_ATCC_15381<br />
<br />
http://aem.asm.org/content/51/4/730.abstract?ijkey=6cfa0071e9fc15e0a007123a571561c86c1354d3&keytype2=tf_ipsecsha<br />
<br />
http://link.springer.com/referencework/10.1007%2F978-3-642-30194-0<br />
<br />
http://jb.asm.org/content/99/1/326.full.pdf <br />
<br />
<br />
=Author=<br />
Dr. Toshi</div>Bgalindo6106https://microbewiki.kenyon.edu/index.php?title=Moritella_marina&diff=127564Moritella marina2016-12-02T21:49:07Z<p>Bgalindo6106: </p>
<hr />
<div>=Classification=<br />
Domain: Bacteria<br />
Phylum: Proteobacteria<br />
Class: Gammaproteobacteria<br />
Order: Alteromonadales<br />
Family: Moritellaceae<br />
Genus: Moritella<br />
<br />
<br />
=Species=<br />
''M. japonica, M. yayanosii, M. viscosa, M. profunda, M. abyssi, M. dasanesis''<br />
<br />
<br />
=Description and Significance=<br />
''Moritella marina'' is a gram-negative halophilic psychrophilic facultative anaerobe with curved or straight rods, motile polar flagella that produce PUFA’s (polyunsaturated fatty acids) and DHA (docosahexanoic acid). “Twenty-five years ago, a marine bacterium originally designated ''Vibrio marinus'' and later renamed ''Moritella marina'' MP-1 was reported to produce high levels of DHA (18% of the total fatty acids). Such high levels of DHA presumably provide this bacterium with the ability to maintain appropriate membrane fluidity in the low temperatures and high pressures of its marine environment.”<br />
“Phylogenetic tree of some representatives of the γ-subclass of the class Proteobacteria showing the position of 16S rDNA sequences from some ''Alteromonas macleodii''-related strains and clone sequences from picoplankton DNA from the Mediterranean Sea mesocosm experiment. Sequences of clones 17B161 and 1B161 are available under accession number Y18229 and Y18231, respectively. The tree was calculated by the algorithm of De Soete [16]. The percentage of 500 bootstrap samplings that support branching points above 80% confidence are indicated. The scale bar corresponds to two nucleotide substitutions over 100 sequence positions. The tree was rooted with ''Roseobacter algicola'' as an outgroup.” (FEMS, 1998)<br />
<br />
[[image:Phytree.png|thumbnail|300px| Figure 1: The Family ''Moritellacae''(Toshi, 2014). ''Moritella viscosa'' is 99% closely related to ''Moritella marina.'' ]]<br />
<br />
=16S Ribosomal RNA Gene Information=<br />
<br />
<br />
[[image:16s.png|thumbnail|300px| Figure 2: Image credit:(Toshi, 2014)]]<br />
<br />
“Sequence variation at positions 73–98 and 181–214 (E. coli nomenclature) within two variable regions of the 16S rDNA of representatives of clone clusters 1–5 and some strains isolated from mesocosm B. Probe target regions are in bold” (FEMS,1998)<br />
<br />
<br />
=Ecology and pathogenesis=<br />
''Moritella marina'' have been found in a variety of cold water marine environments, ranging from the ocean floor to the intestinal tract of marine organisms. Most ''Moritella'' species are thought to live mutualistically amongst larger marine organisms, but ''Moritella viscosa'' has been noted to cause skin ulcers in some fish (Urakawa, 2014). <br />
=Current Research=<br />
''Moritella marina'' has been noted for having unusually high production of the long chain polyunsaturated fatty acids (PUFAs). When ''M. marina'' strain MP-1 was cultured in medium containing cerulenin, a fatty acid synthesis inhibitor, decreases in levels of middle-chain fatty acids and remarkable increases in levels of DHA were observed. These results suggest that the synthesis of middle-chain fatty acids works independently of the synthesis of DHA. M. marina was also found to produce chitonase when induced with chiton (Stefanidi, E., & Vorgias, C. E., 2008). <br />
=References=<br />
<br />
<br />
And, Kumar B. Kautharapu. "Kumar B. Kautharapu." ''Genome Sequence of the Psychrophilic Deep-Sea Bacterium Moritella Marina MP-1'' (ATCC 15381). N.p., 2012. Web. 02 Dec. 2016.<br />
http://jb.asm.org/content/194/22/6296.full<br />
<br />
<br />
Urakawa, H., Kita-Tsukamoto, K., Steven, S. E., Ohwada, K., & Colwell, R. R. (1998). A proposal to transfer Vibrio marinus (Russell 1891) to a new genus Moritella gen. nov. as Moritella marina comb. nov. FEMS microbiology letters, 165(2), 373-378. http://femsle.oxfordjournals.org/content/165/2/373.full#ref-13<br />
<br />
<br />
Pukall, R., Päuker, O., Buntefuß, D., Ulrichs, G., Lebaron, P., Bernard, L., ... & Stackebrandt, E. (1999). High sequence diversity of Alteromonas macleodii-related cloned and cellular 16S rDNAs from a Mediterranean seawater mesocosm experiment. FEMS Microbiology Ecology, 28(4), 335-344.<br />
http://femsec.oxfordjournals.org/content/28/4/335<br />
<br />
<br />
Stefanidi, E., & Vorgias, C. E. (2008). Molecular analysis of the gene encoding a new chitinase from the marine psychrophilic bacterium Moritella marina and biochemical characterization of the recombinant enzyme. Extremophiles, 12(4), 541-552.<br />
"FEMS Microbiology Ecology." High Sequence Diversity of Alteromonas Macleodii-related Cloned and Cellular 16S RDNAs from a Mediterranean Seawater Mesocosm Experiment | FEMS Microbiology Ecology. N.p., n.d. Web. 02 Dec. 2016.<br />
http://femsec.oxfordjournals.org/content/28/4/335 <br />
<br />
<br />
http://www.bacterio.net/moritella.html <br />
<br />
<br />
Genome structure image:<br />
https://www.patricbrc.org/portal/portal/patric/CircosGenomeViewer?cType=genome&cId=1202962.4 <br />
<br />
<br />
http://jb.asm.org/content/194/22/6296.full.pdf<br />
<br />
http://lib.dr.iastate.edu/cgi/viewcontent.cgi?article=1177&context=cbe_pubs<br />
<br />
http://www.biochemsoctrans.org/content/28/6/943.long<br />
<br />
https://www.researchgate.net/publication/232721452_Genome_Sequence_of_the_Psychrophilic_Deep-Sea_Bacterium_Moritella_marina_MP-1_ATCC_15381<br />
<br />
http://aem.asm.org/content/51/4/730.abstract?ijkey=6cfa0071e9fc15e0a007123a571561c86c1354d3&keytype2=tf_ipsecsha<br />
<br />
http://link.springer.com/referencework/10.1007%2F978-3-642-30194-0<br />
<br />
http://jb.asm.org/content/99/1/326.full.pdf <br />
<br />
<br />
=Author=<br />
Dr. Toshi</div>Bgalindo6106https://microbewiki.kenyon.edu/index.php?title=Moritella_marina&diff=127563Moritella marina2016-12-02T21:48:03Z<p>Bgalindo6106: /* Author */</p>
<hr />
<div>=Classification=<br />
Domain: Bacteria<br />
Phylum: Proteobacteria<br />
Class: Gammaproteobacteria<br />
Order: Alteromonadales<br />
Family: Moritellaceae<br />
Genus: Moritella<br />
<br />
<br />
=Species=<br />
''M. japonica, M. yayanosii, M. viscosa, M. profunda, M. abyssi, M. dasanesis''<br />
<br />
<br />
=Description and Significance=<br />
''Moritella marina'' is a gram-negative halophilic psychrophilic facultative anaerobe with curved or straight rods, motile polar flagella that produce PUFA’s (polyunsaturated fatty acids) and DHA (docosahexanoic acid). “Twenty-five years ago, a marine bacterium originally designated ''Vibrio marinus'' and later renamed ''Moritella marina'' MP-1 was reported to produce high levels of DHA (18% of the total fatty acids). Such high levels of DHA presumably provide this bacterium with the ability to maintain appropriate membrane fluidity in the low temperatures and high pressures of its marine environment.”<br />
“Phylogenetic tree of some representatives of the γ-subclass of the class Proteobacteria showing the position of 16S rDNA sequences from some ''Alteromonas macleodii''-related strains and clone sequences from picoplankton DNA from the Mediterranean Sea mesocosm experiment. Sequences of clones 17B161 and 1B161 are available under accession number Y18229 and Y18231, respectively. The tree was calculated by the algorithm of De Soete [16]. The percentage of 500 bootstrap samplings that support branching points above 80% confidence are indicated. The scale bar corresponds to two nucleotide substitutions over 100 sequence positions. The tree was rooted with ''Roseobacter algicola'' as an outgroup.” (FEMS, 1998)<br />
<br />
[[image:Phytree.png|thumbnail|300px| Figure 1: The Family ''Moritellacae''(Toshi, 2014). ''Moritella viscosa'' is 99% closely related to ''Moritella marina.'' ]]<br />
<br />
=16S Ribosomal RNA Gene Information=<br />
<br />
<br />
[[image:16s.png|thumbnail|300px| Figure 2: Image credit:(Toshi, 2014)]]<br />
<br />
“Sequence variation at positions 73–98 and 181–214 (E. coli nomenclature) within two variable regions of the 16S rDNA of representatives of clone clusters 1–5 and some strains isolated from mesocosm B. Probe target regions are in bold” (FEMS,1998)<br />
<br />
=Genome Structure=<br />
<br />
<br />
<br />
Genome structure image link:<br />
https://www.patricbrc.org/portal/portal/patric/CircosGenomeViewer?cType=genome&cId=1202962.4 <br />
Pukall, R., Päuker, O., Buntefuß, D., Ulrichs, G., Lebaron, P., Bernard, L., ... & Stackebrandt, E. (1999). High sequence diversity of Alteromonas macleodii-related cloned and cellular 16S rDNAs from a Mediterranean seawater mesocosm experiment. ''FEMS Microbiology Ecology'', 28(4), 335-344.<br />
http://femsec.oxfordjournals.org/content/28/4/335<br />
<br />
[[image:GenomeStructure.png|thumbnail|375px| Figure 3:High sequence diversity of Alteromonas macleodii-related cloned and cellular 16S rDNAs from a Mediterranean seawater mesocosm experiment. Image credit:''FEMS Microbiology Ecology'']]<br />
<br />
=Ecology and pathogenesis=<br />
''Moritella marina'' have been found in a variety of cold water marine environments, ranging from the ocean floor to the intestinal tract of marine organisms. Most ''Moritella'' species are thought to live mutualistically amongst larger marine organisms, but ''Moritella viscosa'' has been noted to cause skin ulcers in some fish (Urakawa, 2014). <br />
=Current Research=<br />
''Moritella marina'' has been noted for having unusually high production of the long chain polyunsaturated fatty acids (PUFAs). When ''M. marina'' strain MP-1 was cultured in medium containing cerulenin, a fatty acid synthesis inhibitor, decreases in levels of middle-chain fatty acids and remarkable increases in levels of DHA were observed. These results suggest that the synthesis of middle-chain fatty acids works independently of the synthesis of DHA. M. marina was also found to produce chitonase when induced with chiton (Stefanidi, E., & Vorgias, C. E., 2008). <br />
=References=<br />
<br />
<br />
And, Kumar B. Kautharapu. "Kumar B. Kautharapu." ''Genome Sequence of the Psychrophilic Deep-Sea Bacterium Moritella Marina MP-1'' (ATCC 15381). N.p., 2012. Web. 02 Dec. 2016.<br />
http://jb.asm.org/content/194/22/6296.full<br />
<br />
<br />
Urakawa, H., Kita-Tsukamoto, K., Steven, S. E., Ohwada, K., & Colwell, R. R. (1998). A proposal to transfer Vibrio marinus (Russell 1891) to a new genus Moritella gen. nov. as Moritella marina comb. nov. FEMS microbiology letters, 165(2), 373-378. http://femsle.oxfordjournals.org/content/165/2/373.full#ref-13<br />
<br />
<br />
Pukall, R., Päuker, O., Buntefuß, D., Ulrichs, G., Lebaron, P., Bernard, L., ... & Stackebrandt, E. (1999). High sequence diversity of Alteromonas macleodii-related cloned and cellular 16S rDNAs from a Mediterranean seawater mesocosm experiment. FEMS Microbiology Ecology, 28(4), 335-344.<br />
http://femsec.oxfordjournals.org/content/28/4/335<br />
<br />
<br />
Stefanidi, E., & Vorgias, C. E. (2008). Molecular analysis of the gene encoding a new chitinase from the marine psychrophilic bacterium Moritella marina and biochemical characterization of the recombinant enzyme. Extremophiles, 12(4), 541-552.<br />
"FEMS Microbiology Ecology." High Sequence Diversity of Alteromonas Macleodii-related Cloned and Cellular 16S RDNAs from a Mediterranean Seawater Mesocosm Experiment | FEMS Microbiology Ecology. N.p., n.d. Web. 02 Dec. 2016.<br />
http://femsec.oxfordjournals.org/content/28/4/335 <br />
<br />
<br />
http://www.bacterio.net/moritella.html <br />
<br />
<br />
Genome structure image:<br />
https://www.patricbrc.org/portal/portal/patric/CircosGenomeViewer?cType=genome&cId=1202962.4 <br />
<br />
<br />
http://jb.asm.org/content/194/22/6296.full.pdf<br />
<br />
http://lib.dr.iastate.edu/cgi/viewcontent.cgi?article=1177&context=cbe_pubs<br />
<br />
http://www.biochemsoctrans.org/content/28/6/943.long<br />
<br />
https://www.researchgate.net/publication/232721452_Genome_Sequence_of_the_Psychrophilic_Deep-Sea_Bacterium_Moritella_marina_MP-1_ATCC_15381<br />
<br />
http://aem.asm.org/content/51/4/730.abstract?ijkey=6cfa0071e9fc15e0a007123a571561c86c1354d3&keytype2=tf_ipsecsha<br />
<br />
http://link.springer.com/referencework/10.1007%2F978-3-642-30194-0<br />
<br />
http://jb.asm.org/content/99/1/326.full.pdf <br />
<br />
<br />
=Author=<br />
Dr. Toshi</div>Bgalindo6106https://microbewiki.kenyon.edu/index.php?title=Moritella_marina&diff=127560Moritella marina2016-12-02T21:47:46Z<p>Bgalindo6106: </p>
<hr />
<div>=Classification=<br />
Domain: Bacteria<br />
Phylum: Proteobacteria<br />
Class: Gammaproteobacteria<br />
Order: Alteromonadales<br />
Family: Moritellaceae<br />
Genus: Moritella<br />
<br />
<br />
=Species=<br />
''M. japonica, M. yayanosii, M. viscosa, M. profunda, M. abyssi, M. dasanesis''<br />
<br />
<br />
=Description and Significance=<br />
''Moritella marina'' is a gram-negative halophilic psychrophilic facultative anaerobe with curved or straight rods, motile polar flagella that produce PUFA’s (polyunsaturated fatty acids) and DHA (docosahexanoic acid). “Twenty-five years ago, a marine bacterium originally designated ''Vibrio marinus'' and later renamed ''Moritella marina'' MP-1 was reported to produce high levels of DHA (18% of the total fatty acids). Such high levels of DHA presumably provide this bacterium with the ability to maintain appropriate membrane fluidity in the low temperatures and high pressures of its marine environment.”<br />
“Phylogenetic tree of some representatives of the γ-subclass of the class Proteobacteria showing the position of 16S rDNA sequences from some ''Alteromonas macleodii''-related strains and clone sequences from picoplankton DNA from the Mediterranean Sea mesocosm experiment. Sequences of clones 17B161 and 1B161 are available under accession number Y18229 and Y18231, respectively. The tree was calculated by the algorithm of De Soete [16]. The percentage of 500 bootstrap samplings that support branching points above 80% confidence are indicated. The scale bar corresponds to two nucleotide substitutions over 100 sequence positions. The tree was rooted with ''Roseobacter algicola'' as an outgroup.” (FEMS, 1998)<br />
<br />
[[image:Phytree.png|thumbnail|300px| Figure 1: The Family ''Moritellacae''(Toshi, 2014). ''Moritella viscosa'' is 99% closely related to ''Moritella marina.'' ]]<br />
<br />
=16S Ribosomal RNA Gene Information=<br />
<br />
<br />
[[image:16s.png|thumbnail|300px| Figure 2: Image credit:(Toshi, 2014)]]<br />
<br />
“Sequence variation at positions 73–98 and 181–214 (E. coli nomenclature) within two variable regions of the 16S rDNA of representatives of clone clusters 1–5 and some strains isolated from mesocosm B. Probe target regions are in bold” (FEMS,1998)<br />
<br />
=Genome Structure=<br />
<br />
<br />
<br />
Genome structure image link:<br />
https://www.patricbrc.org/portal/portal/patric/CircosGenomeViewer?cType=genome&cId=1202962.4 <br />
Pukall, R., Päuker, O., Buntefuß, D., Ulrichs, G., Lebaron, P., Bernard, L., ... & Stackebrandt, E. (1999). High sequence diversity of Alteromonas macleodii-related cloned and cellular 16S rDNAs from a Mediterranean seawater mesocosm experiment. ''FEMS Microbiology Ecology'', 28(4), 335-344.<br />
http://femsec.oxfordjournals.org/content/28/4/335<br />
<br />
[[image:GenomeStructure.png|thumbnail|375px| Figure 3:High sequence diversity of Alteromonas macleodii-related cloned and cellular 16S rDNAs from a Mediterranean seawater mesocosm experiment. Image credit:''FEMS Microbiology Ecology'']]<br />
<br />
=Ecology and pathogenesis=<br />
''Moritella marina'' have been found in a variety of cold water marine environments, ranging from the ocean floor to the intestinal tract of marine organisms. Most ''Moritella'' species are thought to live mutualistically amongst larger marine organisms, but ''Moritella viscosa'' has been noted to cause skin ulcers in some fish (Urakawa, 2014). <br />
=Current Research=<br />
''Moritella marina'' has been noted for having unusually high production of the long chain polyunsaturated fatty acids (PUFAs). When ''M. marina'' strain MP-1 was cultured in medium containing cerulenin, a fatty acid synthesis inhibitor, decreases in levels of middle-chain fatty acids and remarkable increases in levels of DHA were observed. These results suggest that the synthesis of middle-chain fatty acids works independently of the synthesis of DHA. M. marina was also found to produce chitonase when induced with chiton (Stefanidi, E., & Vorgias, C. E., 2008). <br />
=References=<br />
<br />
<br />
And, Kumar B. Kautharapu. "Kumar B. Kautharapu." ''Genome Sequence of the Psychrophilic Deep-Sea Bacterium Moritella Marina MP-1'' (ATCC 15381). N.p., 2012. Web. 02 Dec. 2016.<br />
http://jb.asm.org/content/194/22/6296.full<br />
<br />
<br />
Urakawa, H., Kita-Tsukamoto, K., Steven, S. E., Ohwada, K., & Colwell, R. R. (1998). A proposal to transfer Vibrio marinus (Russell 1891) to a new genus Moritella gen. nov. as Moritella marina comb. nov. FEMS microbiology letters, 165(2), 373-378. http://femsle.oxfordjournals.org/content/165/2/373.full#ref-13<br />
<br />
<br />
Pukall, R., Päuker, O., Buntefuß, D., Ulrichs, G., Lebaron, P., Bernard, L., ... & Stackebrandt, E. (1999). High sequence diversity of Alteromonas macleodii-related cloned and cellular 16S rDNAs from a Mediterranean seawater mesocosm experiment. FEMS Microbiology Ecology, 28(4), 335-344.<br />
http://femsec.oxfordjournals.org/content/28/4/335<br />
<br />
<br />
Stefanidi, E., & Vorgias, C. E. (2008). Molecular analysis of the gene encoding a new chitinase from the marine psychrophilic bacterium Moritella marina and biochemical characterization of the recombinant enzyme. Extremophiles, 12(4), 541-552.<br />
"FEMS Microbiology Ecology." High Sequence Diversity of Alteromonas Macleodii-related Cloned and Cellular 16S RDNAs from a Mediterranean Seawater Mesocosm Experiment | FEMS Microbiology Ecology. N.p., n.d. Web. 02 Dec. 2016.<br />
http://femsec.oxfordjournals.org/content/28/4/335 <br />
<br />
<br />
http://www.bacterio.net/moritella.html <br />
<br />
<br />
Genome structure image:<br />
https://www.patricbrc.org/portal/portal/patric/CircosGenomeViewer?cType=genome&cId=1202962.4 <br />
<br />
<br />
http://jb.asm.org/content/194/22/6296.full.pdf<br />
<br />
http://lib.dr.iastate.edu/cgi/viewcontent.cgi?article=1177&context=cbe_pubs<br />
<br />
http://www.biochemsoctrans.org/content/28/6/943.long<br />
<br />
https://www.researchgate.net/publication/232721452_Genome_Sequence_of_the_Psychrophilic_Deep-Sea_Bacterium_Moritella_marina_MP-1_ATCC_15381<br />
<br />
http://aem.asm.org/content/51/4/730.abstract?ijkey=6cfa0071e9fc15e0a007123a571561c86c1354d3&keytype2=tf_ipsecsha<br />
<br />
http://link.springer.com/referencework/10.1007%2F978-3-642-30194-0<br />
<br />
http://jb.asm.org/content/99/1/326.full.pdf <br />
<br />
<br />
=Author=<br />
toshi</div>Bgalindo6106https://microbewiki.kenyon.edu/index.php?title=Moritella_marina&diff=127559Moritella marina2016-12-02T21:47:18Z<p>Bgalindo6106: </p>
<hr />
<div>=Classification=<br />
Domain: Bacteria<br />
Phylum: Proteobacteria<br />
Class: Gammaproteobacteria<br />
Order: Alteromonadales<br />
Family: Moritellaceae<br />
Genus: Moritella<br />
<br />
<br />
=Species=<br />
''M. japonica, M. yayanosii, M. viscosa, M. profunda, M. abyssi, M. dasanesis''<br />
<br />
<br />
=Description and Significance=<br />
''Moritella marina'' is a gram-negative halophilic psychrophilic facultative anaerobe with curved or straight rods, motile polar flagella that produce PUFA’s (polyunsaturated fatty acids) and DHA (docosahexanoic acid). “Twenty-five years ago, a marine bacterium originally designated ''Vibrio marinus'' and later renamed ''Moritella marina'' MP-1 was reported to produce high levels of DHA (18% of the total fatty acids). Such high levels of DHA presumably provide this bacterium with the ability to maintain appropriate membrane fluidity in the low temperatures and high pressures of its marine environment.”<br />
“Phylogenetic tree of some representatives of the γ-subclass of the class Proteobacteria showing the position of 16S rDNA sequences from some ''Alteromonas macleodii''-related strains and clone sequences from picoplankton DNA from the Mediterranean Sea mesocosm experiment. Sequences of clones 17B161 and 1B161 are available under accession number Y18229 and Y18231, respectively. The tree was calculated by the algorithm of De Soete [16]. The percentage of 500 bootstrap samplings that support branching points above 80% confidence are indicated. The scale bar corresponds to two nucleotide substitutions over 100 sequence positions. The tree was rooted with ''Roseobacter algicola'' as an outgroup.” (FEMS, 1998)<br />
<br />
[[image:Phytree.png|thumbnail|300px| Figure 1: The Family ''Moritellacae''(Toshi, 2014). ''Moritella viscosa'' is 99% closely related to ''Moritella marina.'' ]]<br />
<br />
=16S Ribosomal RNA Gene Information=<br />
<br />
<br />
[[image:16s.png|thumbnail|300px| Figure 2: Image credit:(Toshi, 2014)]]<br />
<br />
“Sequence variation at positions 73–98 and 181–214 (E. coli nomenclature) within two variable regions of the 16S rDNA of representatives of clone clusters 1–5 and some strains isolated from mesocosm B. Probe target regions are in bold” (FEMS,1998)<br />
<br />
=Genome Structure=<br />
<br />
<br />
<br />
Genome structure image link:<br />
https://www.patricbrc.org/portal/portal/patric/CircosGenomeViewer?cType=genome&cId=1202962.4 <br />
Pukall, R., Päuker, O., Buntefuß, D., Ulrichs, G., Lebaron, P., Bernard, L., ... & Stackebrandt, E. (1999). High sequence diversity of Alteromonas macleodii-related cloned and cellular 16S rDNAs from a Mediterranean seawater mesocosm experiment. ''FEMS Microbiology Ecology'', 28(4), 335-344.<br />
http://femsec.oxfordjournals.org/content/28/4/335<br />
<br />
[[image:GenomeStructure.png|thumbnail|375px| Figure 3:High sequence diversity of Alteromonas macleodii-related cloned and cellular 16S rDNAs from a Mediterranean seawater mesocosm experiment. Image credit:''FEMS Microbiology Ecology'']]<br />
<br />
=Ecology and pathogenesis=<br />
''Moritella marina'' have been found in a variety of cold water marine environments, ranging from the ocean floor to the intestinal tract of marine organisms. Most ''Moritella'' species are thought to live mutualistically amongst larger marine organisms, but ''Moritella viscosa'' has been noted to cause skin ulcers in some fish (Urakawa, 2014). <br />
=Current Research=<br />
''Moritella marina'' has been noted for having unusually high production of the long chain polyunsaturated fatty acids (PUFAs). When ''M. marina'' strain MP-1 was cultured in medium containing cerulenin, a fatty acid synthesis inhibitor, decreases in levels of middle-chain fatty acids and remarkable increases in levels of DHA were observed. These results suggest that the synthesis of middle-chain fatty acids works independently of the synthesis of DHA. M. marina was also found to produce chitonase when induced with chiton (Stefanidi, E., & Vorgias, C. E., 2008). <br />
=References=<br />
<br />
<br />
And, Kumar B. Kautharapu. "Kumar B. Kautharapu." ''Genome Sequence of the Psychrophilic Deep-Sea Bacterium Moritella Marina MP-1'' (ATCC 15381). N.p., 2012. Web. 02 Dec. 2016.<br />
http://jb.asm.org/content/194/22/6296.full<br />
<br />
<br />
Urakawa, H., Kita-Tsukamoto, K., Steven, S. E., Ohwada, K., & Colwell, R. R. (1998). A proposal to transfer Vibrio marinus (Russell 1891) to a new genus Moritella gen. nov. as Moritella marina comb. nov. FEMS microbiology letters, 165(2), 373-378. http://femsle.oxfordjournals.org/content/165/2/373.full#ref-13<br />
<br />
<br />
Pukall, R., Päuker, O., Buntefuß, D., Ulrichs, G., Lebaron, P., Bernard, L., ... & Stackebrandt, E. (1999). High sequence diversity of Alteromonas macleodii-related cloned and cellular 16S rDNAs from a Mediterranean seawater mesocosm experiment. FEMS Microbiology Ecology, 28(4), 335-344.<br />
http://femsec.oxfordjournals.org/content/28/4/335<br />
<br />
<br />
Stefanidi, E., & Vorgias, C. E. (2008). Molecular analysis of the gene encoding a new chitinase from the marine psychrophilic bacterium Moritella marina and biochemical characterization of the recombinant enzyme. Extremophiles, 12(4), 541-552.<br />
"FEMS Microbiology Ecology." High Sequence Diversity of Alteromonas Macleodii-related Cloned and Cellular 16S RDNAs from a Mediterranean Seawater Mesocosm Experiment | FEMS Microbiology Ecology. N.p., n.d. Web. 02 Dec. 2016.<br />
http://femsec.oxfordjournals.org/content/28/4/335 <br />
<br />
<br />
http://www.bacterio.net/moritella.html <br />
<br />
<br />
Genome structure image:<br />
https://www.patricbrc.org/portal/portal/patric/CircosGenomeViewer?cType=genome&cId=1202962.4 <br />
<br />
<br />
http://jb.asm.org/content/194/22/6296.full.pdf<br />
<br />
http://lib.dr.iastate.edu/cgi/viewcontent.cgi?article=1177&context=cbe_pubs<br />
<br />
http://www.biochemsoctrans.org/content/28/6/943.long<br />
<br />
https://www.researchgate.net/publication/232721452_Genome_Sequence_of_the_Psychrophilic_Deep-Sea_Bacterium_Moritella_marina_MP-1_ATCC_15381<br />
<br />
http://aem.asm.org/content/51/4/730.abstract?ijkey=6cfa0071e9fc15e0a007123a571561c86c1354d3&keytype2=tf_ipsecsha<br />
<br />
http://link.springer.com/referencework/10.1007%2F978-3-642-30194-0<br />
<br />
http://jb.asm.org/content/99/1/326.full.pdf <br />
<br />
<br />
Author</div>Bgalindo6106https://microbewiki.kenyon.edu/index.php?title=Moritella_marina&diff=127556Moritella marina2016-12-02T21:45:59Z<p>Bgalindo6106: /* Description and Significance */</p>
<hr />
<div>=Classification=<br />
Domain: Bacteria<br />
Phylum: Proteobacteria<br />
Class: Gammaproteobacteria<br />
Order: Alteromonadales<br />
Family: Moritellaceae<br />
Genus: Moritella<br />
<br />
<br />
=Species=<br />
''M. japonica, M. yayanosii, M. viscosa, M. profunda, M. abyssi, M. dasanesis''<br />
<br />
<br />
=Description and Significance=<br />
''Moritella marina'' is a gram-negative halophilic psychrophilic facultative anaerobe with curved or straight rods, motile polar flagella that produce PUFA’s (polyunsaturated fatty acids) and DHA (docosahexanoic acid). “Twenty-five years ago, a marine bacterium originally designated ''Vibrio marinus'' and later renamed ''Moritella marina'' MP-1 was reported to produce high levels of DHA (18% of the total fatty acids). Such high levels of DHA presumably provide this bacterium with the ability to maintain appropriate membrane fluidity in the low temperatures and high pressures of its marine environment.”<br />
“Phylogenetic tree of some representatives of the γ-subclass of the class Proteobacteria showing the position of 16S rDNA sequences from some ''Alteromonas macleodii''-related strains and clone sequences from picoplankton DNA from the Mediterranean Sea mesocosm experiment. Sequences of clones 17B161 and 1B161 are available under accession number Y18229 and Y18231, respectively. The tree was calculated by the algorithm of De Soete [16]. The percentage of 500 bootstrap samplings that support branching points above 80% confidence are indicated. The scale bar corresponds to two nucleotide substitutions over 100 sequence positions. The tree was rooted with ''Roseobacter algicola'' as an outgroup.” (FEMS, 1998)<br />
<br />
[[image:Phytree.png|thumbnail|300px| Figure 1: The Family ''Moritellacae''(Toshi, 2014). ''Moritella viscosa'' is 99% closely related to ''Moritella marina.'' ]]<br />
<br />
=16S Ribosomal RNA Gene Information=<br />
<br />
<br />
[[image:16s.png|thumbnail|300px| Figure 2: Image credit:(Toshi, 2014)]]<br />
<br />
“Sequence variation at positions 73–98 and 181–214 (E. coli nomenclature) within two variable regions of the 16S rDNA of representatives of clone clusters 1–5 and some strains isolated from mesocosm B. Probe target regions are in bold” (FEMS,1998)<br />
<br />
=Genome Structure=<br />
<br />
<br />
<br />
Genome structure image link:<br />
https://www.patricbrc.org/portal/portal/patric/CircosGenomeViewer?cType=genome&cId=1202962.4 <br />
Pukall, R., Päuker, O., Buntefuß, D., Ulrichs, G., Lebaron, P., Bernard, L., ... & Stackebrandt, E. (1999). High sequence diversity of Alteromonas macleodii-related cloned and cellular 16S rDNAs from a Mediterranean seawater mesocosm experiment. ''FEMS Microbiology Ecology'', 28(4), 335-344.<br />
http://femsec.oxfordjournals.org/content/28/4/335<br />
<br />
[[image:GenomeStructure.png|thumbnail|375px| Figure 3:High sequence diversity of Alteromonas macleodii-related cloned and cellular 16S rDNAs from a Mediterranean seawater mesocosm experiment. Image credit:''FEMS Microbiology Ecology'']]<br />
<br />
=Ecology and pathogenesis=<br />
''Moritella marina'' have been found in a variety of cold water marine environments, ranging from the ocean floor to the intestinal tract of marine organisms. Most ''Moritella'' species are thought to live mutualistically amongst larger marine organisms, but ''Moritella viscosa'' has been noted to cause skin ulcers in some fish (Urakawa, 2014). <br />
=Current Research=<br />
''Moritella marina'' has been noted for having unusually high production of the long chain polyunsaturated fatty acids (PUFAs). When ''M. marina'' strain MP-1 was cultured in medium containing cerulenin, a fatty acid synthesis inhibitor, decreases in levels of middle-chain fatty acids and remarkable increases in levels of DHA were observed. These results suggest that the synthesis of middle-chain fatty acids works independently of the synthesis of DHA. M. marina was also found to produce chitonase when induced with chiton (citation). <br />
References<br />
<br />
<br />
And, Kumar B. Kautharapu. "Kumar B. Kautharapu." ''Genome Sequence of the Psychrophilic Deep-Sea Bacterium Moritella Marina MP-1'' (ATCC 15381). N.p., 2012. Web. 02 Dec. 2016.<br />
http://jb.asm.org/content/194/22/6296.full<br />
<br />
<br />
Urakawa, H., Kita-Tsukamoto, K., Steven, S. E., Ohwada, K., & Colwell, R. R. (1998). A proposal to transfer Vibrio marinus (Russell 1891) to a new genus Moritella gen. nov. as Moritella marina comb. nov. FEMS microbiology letters, 165(2), 373-378. http://femsle.oxfordjournals.org/content/165/2/373.full#ref-13<br />
<br />
<br />
Pukall, R., Päuker, O., Buntefuß, D., Ulrichs, G., Lebaron, P., Bernard, L., ... & Stackebrandt, E. (1999). High sequence diversity of Alteromonas macleodii-related cloned and cellular 16S rDNAs from a Mediterranean seawater mesocosm experiment. FEMS Microbiology Ecology, 28(4), 335-344.<br />
http://femsec.oxfordjournals.org/content/28/4/335<br />
<br />
<br />
Stefanidi, E., & Vorgias, C. E. (2008). Molecular analysis of the gene encoding a new chitinase from the marine psychrophilic bacterium Moritella marina and biochemical characterization of the recombinant enzyme. Extremophiles, 12(4), 541-552.<br />
"FEMS Microbiology Ecology." High Sequence Diversity of Alteromonas Macleodii-related Cloned and Cellular 16S RDNAs from a Mediterranean Seawater Mesocosm Experiment | FEMS Microbiology Ecology. N.p., n.d. Web. 02 Dec. 2016.<br />
http://femsec.oxfordjournals.org/content/28/4/335 <br />
<br />
<br />
http://www.bacterio.net/moritella.html <br />
<br />
<br />
Genome structure image:<br />
https://www.patricbrc.org/portal/portal/patric/CircosGenomeViewer?cType=genome&cId=1202962.4 <br />
<br />
<br />
http://jb.asm.org/content/194/22/6296.full.pdf<br />
<br />
http://lib.dr.iastate.edu/cgi/viewcontent.cgi?article=1177&context=cbe_pubs<br />
<br />
http://www.biochemsoctrans.org/content/28/6/943.long<br />
<br />
https://www.researchgate.net/publication/232721452_Genome_Sequence_of_the_Psychrophilic_Deep-Sea_Bacterium_Moritella_marina_MP-1_ATCC_15381<br />
<br />
http://aem.asm.org/content/51/4/730.abstract?ijkey=6cfa0071e9fc15e0a007123a571561c86c1354d3&keytype2=tf_ipsecsha<br />
<br />
http://link.springer.com/referencework/10.1007%2F978-3-642-30194-0<br />
<br />
http://jb.asm.org/content/99/1/326.full.pdf <br />
<br />
<br />
Author</div>Bgalindo6106https://microbewiki.kenyon.edu/index.php?title=Moritella_marina&diff=127554Moritella marina2016-12-02T21:44:06Z<p>Bgalindo6106: /* 16S Ribosomal RNA Gene Information */</p>
<hr />
<div>=Classification=<br />
Domain: Bacteria<br />
Phylum: Proteobacteria<br />
Class: Gammaproteobacteria<br />
Order: Alteromonadales<br />
Family: Moritellaceae<br />
Genus: Moritella<br />
<br />
<br />
=Species=<br />
''M. japonica, M. yayanosii, M. viscosa, M. profunda, M. abyssi, M. dasanesis''<br />
<br />
<br />
=Description and Significance=<br />
''Moritella marina'' is a gram-negative halophilic psychrophilic facultative anaerobe with curved or straight rods, motile polar flagella that produce PUFA’s (polyunsaturated fatty acids) and DHA (docosahexanoic acid). “Twenty-five years ago, a marine bacterium originally designated ''Vibrio marinus'' (3) and later renamed ''Moritella marina'' MP-1 (14) was reported to produce high levels of DHA (18% of the total fatty acids) (4). Such high levels of DHA presumably provide this bacterium with the ability to maintain appropriate membrane fluidity in the low temperatures and high pressures of its marine environment.”<br />
“Phylogenetic tree of some representatives of the γ-subclass of the class Proteobacteria showing the position of 16S rDNA sequences from some ''Alteromonas macleodii''-related strains and clone sequences from picoplankton DNA from the Mediterranean Sea mesocosm experiment. Sequences of clones 17B161 and 1B161 are available under accession number Y18229 and Y18231, respectively. The tree was calculated by the algorithm of De Soete [16]. The percentage of 500 bootstrap samplings that support branching points above 80% confidence are indicated. The scale bar corresponds to two nucleotide substitutions over 100 sequence positions. The tree was rooted with ''Roseobacter algicola'' as an outgroup.” (FEMS, 1998)<br />
<br />
[[image:Phytree.png|thumbnail|300px| Figure 1: The Family ''Moritellacae''(Toshi, 2014). ''Moritella viscosa'' is 99% closely related to ''Moritella marina.'' ]]<br />
<br />
=16S Ribosomal RNA Gene Information=<br />
<br />
<br />
[[image:16s.png|thumbnail|300px| Figure 2: Image credit:(Toshi, 2014)]]<br />
<br />
“Sequence variation at positions 73–98 and 181–214 (E. coli nomenclature) within two variable regions of the 16S rDNA of representatives of clone clusters 1–5 and some strains isolated from mesocosm B. Probe target regions are in bold” (FEMS,1998)<br />
<br />
=Genome Structure=<br />
<br />
<br />
<br />
Genome structure image link:<br />
https://www.patricbrc.org/portal/portal/patric/CircosGenomeViewer?cType=genome&cId=1202962.4 <br />
Pukall, R., Päuker, O., Buntefuß, D., Ulrichs, G., Lebaron, P., Bernard, L., ... & Stackebrandt, E. (1999). High sequence diversity of Alteromonas macleodii-related cloned and cellular 16S rDNAs from a Mediterranean seawater mesocosm experiment. ''FEMS Microbiology Ecology'', 28(4), 335-344.<br />
http://femsec.oxfordjournals.org/content/28/4/335<br />
<br />
[[image:GenomeStructure.png|thumbnail|375px| Figure 3:High sequence diversity of Alteromonas macleodii-related cloned and cellular 16S rDNAs from a Mediterranean seawater mesocosm experiment. Image credit:''FEMS Microbiology Ecology'']]<br />
<br />
=Ecology and pathogenesis=<br />
''Moritella marina'' have been found in a variety of cold water marine environments, ranging from the ocean floor to the intestinal tract of marine organisms. Most ''Moritella'' species are thought to live mutualistically amongst larger marine organisms, but ''Moritella viscosa'' has been noted to cause skin ulcers in some fish (Urakawa, 2014). <br />
=Current Research=<br />
''Moritella marina'' has been noted for having unusually high production of the long chain polyunsaturated fatty acids (PUFAs). When ''M. marina'' strain MP-1 was cultured in medium containing cerulenin, a fatty acid synthesis inhibitor, decreases in levels of middle-chain fatty acids and remarkable increases in levels of DHA were observed. These results suggest that the synthesis of middle-chain fatty acids works independently of the synthesis of DHA. M. marina was also found to produce chitonase when induced with chiton (citation). <br />
References<br />
<br />
<br />
And, Kumar B. Kautharapu. "Kumar B. Kautharapu." ''Genome Sequence of the Psychrophilic Deep-Sea Bacterium Moritella Marina MP-1'' (ATCC 15381). N.p., 2012. Web. 02 Dec. 2016.<br />
http://jb.asm.org/content/194/22/6296.full<br />
<br />
<br />
Urakawa, H., Kita-Tsukamoto, K., Steven, S. E., Ohwada, K., & Colwell, R. R. (1998). A proposal to transfer Vibrio marinus (Russell 1891) to a new genus Moritella gen. nov. as Moritella marina comb. nov. FEMS microbiology letters, 165(2), 373-378. http://femsle.oxfordjournals.org/content/165/2/373.full#ref-13<br />
<br />
<br />
Pukall, R., Päuker, O., Buntefuß, D., Ulrichs, G., Lebaron, P., Bernard, L., ... & Stackebrandt, E. (1999). High sequence diversity of Alteromonas macleodii-related cloned and cellular 16S rDNAs from a Mediterranean seawater mesocosm experiment. FEMS Microbiology Ecology, 28(4), 335-344.<br />
http://femsec.oxfordjournals.org/content/28/4/335<br />
<br />
<br />
Stefanidi, E., & Vorgias, C. E. (2008). Molecular analysis of the gene encoding a new chitinase from the marine psychrophilic bacterium Moritella marina and biochemical characterization of the recombinant enzyme. Extremophiles, 12(4), 541-552.<br />
"FEMS Microbiology Ecology." High Sequence Diversity of Alteromonas Macleodii-related Cloned and Cellular 16S RDNAs from a Mediterranean Seawater Mesocosm Experiment | FEMS Microbiology Ecology. N.p., n.d. Web. 02 Dec. 2016.<br />
http://femsec.oxfordjournals.org/content/28/4/335 <br />
<br />
<br />
http://www.bacterio.net/moritella.html <br />
<br />
<br />
Genome structure image:<br />
https://www.patricbrc.org/portal/portal/patric/CircosGenomeViewer?cType=genome&cId=1202962.4 <br />
<br />
<br />
http://jb.asm.org/content/194/22/6296.full.pdf<br />
<br />
http://lib.dr.iastate.edu/cgi/viewcontent.cgi?article=1177&context=cbe_pubs<br />
<br />
http://www.biochemsoctrans.org/content/28/6/943.long<br />
<br />
https://www.researchgate.net/publication/232721452_Genome_Sequence_of_the_Psychrophilic_Deep-Sea_Bacterium_Moritella_marina_MP-1_ATCC_15381<br />
<br />
http://aem.asm.org/content/51/4/730.abstract?ijkey=6cfa0071e9fc15e0a007123a571561c86c1354d3&keytype2=tf_ipsecsha<br />
<br />
http://link.springer.com/referencework/10.1007%2F978-3-642-30194-0<br />
<br />
http://jb.asm.org/content/99/1/326.full.pdf <br />
<br />
<br />
Author</div>Bgalindo6106https://microbewiki.kenyon.edu/index.php?title=Moritella_marina&diff=127553Moritella marina2016-12-02T21:42:36Z<p>Bgalindo6106: /* Description and Significance */</p>
<hr />
<div>=Classification=<br />
Domain: Bacteria<br />
Phylum: Proteobacteria<br />
Class: Gammaproteobacteria<br />
Order: Alteromonadales<br />
Family: Moritellaceae<br />
Genus: Moritella<br />
<br />
<br />
=Species=<br />
''M. japonica, M. yayanosii, M. viscosa, M. profunda, M. abyssi, M. dasanesis''<br />
<br />
<br />
=Description and Significance=<br />
''Moritella marina'' is a gram-negative halophilic psychrophilic facultative anaerobe with curved or straight rods, motile polar flagella that produce PUFA’s (polyunsaturated fatty acids) and DHA (docosahexanoic acid). “Twenty-five years ago, a marine bacterium originally designated ''Vibrio marinus'' (3) and later renamed ''Moritella marina'' MP-1 (14) was reported to produce high levels of DHA (18% of the total fatty acids) (4). Such high levels of DHA presumably provide this bacterium with the ability to maintain appropriate membrane fluidity in the low temperatures and high pressures of its marine environment.”<br />
“Phylogenetic tree of some representatives of the γ-subclass of the class Proteobacteria showing the position of 16S rDNA sequences from some ''Alteromonas macleodii''-related strains and clone sequences from picoplankton DNA from the Mediterranean Sea mesocosm experiment. Sequences of clones 17B161 and 1B161 are available under accession number Y18229 and Y18231, respectively. The tree was calculated by the algorithm of De Soete [16]. The percentage of 500 bootstrap samplings that support branching points above 80% confidence are indicated. The scale bar corresponds to two nucleotide substitutions over 100 sequence positions. The tree was rooted with ''Roseobacter algicola'' as an outgroup.” (FEMS, 1998)<br />
<br />
[[image:Phytree.png|thumbnail|300px| Figure 1: The Family ''Moritellacae''(Toshi, 2014). ''Moritella viscosa'' is 99% closely related to ''Moritella marina.'' ]]<br />
<br />
=16S Ribosomal RNA Gene Information=<br />
<br />
<br />
[[image:16s.png|thumbnail|300px| Figure 2:]]<br />
<br />
“Sequence variation at positions 73–98 and 181–214 (E. coli nomenclature) within two variable regions of the 16S rDNA of representatives of clone clusters 1–5 and some strains isolated from mesocosm B. Probe target regions are in bold” (FEMS,1998)<br />
<br />
=Genome Structure=<br />
<br />
<br />
<br />
Genome structure image link:<br />
https://www.patricbrc.org/portal/portal/patric/CircosGenomeViewer?cType=genome&cId=1202962.4 <br />
Pukall, R., Päuker, O., Buntefuß, D., Ulrichs, G., Lebaron, P., Bernard, L., ... & Stackebrandt, E. (1999). High sequence diversity of Alteromonas macleodii-related cloned and cellular 16S rDNAs from a Mediterranean seawater mesocosm experiment. ''FEMS Microbiology Ecology'', 28(4), 335-344.<br />
http://femsec.oxfordjournals.org/content/28/4/335<br />
<br />
[[image:GenomeStructure.png|thumbnail|375px| Figure 3:High sequence diversity of Alteromonas macleodii-related cloned and cellular 16S rDNAs from a Mediterranean seawater mesocosm experiment. Image credit:''FEMS Microbiology Ecology'']]<br />
<br />
=Ecology and pathogenesis=<br />
''Moritella marina'' have been found in a variety of cold water marine environments, ranging from the ocean floor to the intestinal tract of marine organisms. Most ''Moritella'' species are thought to live mutualistically amongst larger marine organisms, but ''Moritella viscosa'' has been noted to cause skin ulcers in some fish (Urakawa, 2014). <br />
=Current Research=<br />
''Moritella marina'' has been noted for having unusually high production of the long chain polyunsaturated fatty acids (PUFAs). When ''M. marina'' strain MP-1 was cultured in medium containing cerulenin, a fatty acid synthesis inhibitor, decreases in levels of middle-chain fatty acids and remarkable increases in levels of DHA were observed. These results suggest that the synthesis of middle-chain fatty acids works independently of the synthesis of DHA. M. marina was also found to produce chitonase when induced with chiton (citation). <br />
References<br />
<br />
<br />
And, Kumar B. Kautharapu. "Kumar B. Kautharapu." ''Genome Sequence of the Psychrophilic Deep-Sea Bacterium Moritella Marina MP-1'' (ATCC 15381). N.p., 2012. Web. 02 Dec. 2016.<br />
http://jb.asm.org/content/194/22/6296.full<br />
<br />
<br />
Urakawa, H., Kita-Tsukamoto, K., Steven, S. E., Ohwada, K., & Colwell, R. R. (1998). A proposal to transfer Vibrio marinus (Russell 1891) to a new genus Moritella gen. nov. as Moritella marina comb. nov. FEMS microbiology letters, 165(2), 373-378. http://femsle.oxfordjournals.org/content/165/2/373.full#ref-13<br />
<br />
<br />
Pukall, R., Päuker, O., Buntefuß, D., Ulrichs, G., Lebaron, P., Bernard, L., ... & Stackebrandt, E. (1999). High sequence diversity of Alteromonas macleodii-related cloned and cellular 16S rDNAs from a Mediterranean seawater mesocosm experiment. FEMS Microbiology Ecology, 28(4), 335-344.<br />
http://femsec.oxfordjournals.org/content/28/4/335<br />
<br />
<br />
Stefanidi, E., & Vorgias, C. E. (2008). Molecular analysis of the gene encoding a new chitinase from the marine psychrophilic bacterium Moritella marina and biochemical characterization of the recombinant enzyme. Extremophiles, 12(4), 541-552.<br />
"FEMS Microbiology Ecology." High Sequence Diversity of Alteromonas Macleodii-related Cloned and Cellular 16S RDNAs from a Mediterranean Seawater Mesocosm Experiment | FEMS Microbiology Ecology. N.p., n.d. Web. 02 Dec. 2016.<br />
http://femsec.oxfordjournals.org/content/28/4/335 <br />
<br />
<br />
http://www.bacterio.net/moritella.html <br />
<br />
<br />
Genome structure image:<br />
https://www.patricbrc.org/portal/portal/patric/CircosGenomeViewer?cType=genome&cId=1202962.4 <br />
<br />
<br />
http://jb.asm.org/content/194/22/6296.full.pdf<br />
<br />
http://lib.dr.iastate.edu/cgi/viewcontent.cgi?article=1177&context=cbe_pubs<br />
<br />
http://www.biochemsoctrans.org/content/28/6/943.long<br />
<br />
https://www.researchgate.net/publication/232721452_Genome_Sequence_of_the_Psychrophilic_Deep-Sea_Bacterium_Moritella_marina_MP-1_ATCC_15381<br />
<br />
http://aem.asm.org/content/51/4/730.abstract?ijkey=6cfa0071e9fc15e0a007123a571561c86c1354d3&keytype2=tf_ipsecsha<br />
<br />
http://link.springer.com/referencework/10.1007%2F978-3-642-30194-0<br />
<br />
http://jb.asm.org/content/99/1/326.full.pdf <br />
<br />
<br />
Author</div>Bgalindo6106https://microbewiki.kenyon.edu/index.php?title=Moritella_marina&diff=127549Moritella marina2016-12-02T21:38:40Z<p>Bgalindo6106: /* Description and Significance */</p>
<hr />
<div>=Classification=<br />
Domain: Bacteria<br />
Phylum: Proteobacteria<br />
Class: Gammaproteobacteria<br />
Order: Alteromonadales<br />
Family: Moritellaceae<br />
Genus: Moritella<br />
<br />
<br />
=Species=<br />
''M. japonica, M. yayanosii, M. viscosa, M. profunda, M. abyssi, M. dasanesis''<br />
<br />
<br />
=Description and Significance=<br />
''Moritella marina'' is a gram-negative halophilic psychrophilic facultative anaerobe with curved or straight rods, motile polar flagella that produce PUFA’s (polyunsaturated fatty acids) and DHA (docosahexanoic acid). “Twenty-five years ago, a marine bacterium originally designated ''Vibrio marinus'' (3) and later renamed ''Moritella marina'' MP-1 (14) was reported to produce high levels of DHA (18% of the total fatty acids) (4). Such high levels of DHA presumably provide this bacterium with the ability to maintain appropriate membrane fluidity in the low temperatures and high pressures of its marine environment.”<br />
“Phylogenetic tree of some representatives of the γ-subclass of the class Proteobacteria showing the position of 16S rDNA sequences from some ''Alteromonas macleodii''-related strains and clone sequences from picoplankton DNA from the Mediterranean Sea mesocosm experiment. Sequences of clones 17B161 and 1B161 are available under accession number Y18229 and Y18231, respectively. The tree was calculated by the algorithm of De Soete [16]. The percentage of 500 bootstrap samplings that support branching points above 80% confidence are indicated. The scale bar corresponds to two nucleotide substitutions over 100 sequence positions. The tree was rooted with ''Roseobacter algicola'' as an outgroup.” (FEMS, 1998)<br />
<br />
[[image:Phytree.png|thumbnail|300px| Figure 1: The Family ''Moritellacae''(Toshi, 2014)]]<br />
<br />
=16S Ribosomal RNA Gene Information=<br />
<br />
<br />
[[image:16s.png|thumbnail|300px| Figure 2:]]<br />
<br />
“Sequence variation at positions 73–98 and 181–214 (E. coli nomenclature) within two variable regions of the 16S rDNA of representatives of clone clusters 1–5 and some strains isolated from mesocosm B. Probe target regions are in bold” (FEMS,1998)<br />
<br />
=Genome Structure=<br />
<br />
<br />
<br />
Genome structure image link:<br />
https://www.patricbrc.org/portal/portal/patric/CircosGenomeViewer?cType=genome&cId=1202962.4 <br />
Pukall, R., Päuker, O., Buntefuß, D., Ulrichs, G., Lebaron, P., Bernard, L., ... & Stackebrandt, E. (1999). High sequence diversity of Alteromonas macleodii-related cloned and cellular 16S rDNAs from a Mediterranean seawater mesocosm experiment. ''FEMS Microbiology Ecology'', 28(4), 335-344.<br />
http://femsec.oxfordjournals.org/content/28/4/335<br />
<br />
[[image:GenomeStructure.png|thumbnail|375px| Figure 3:High sequence diversity of Alteromonas macleodii-related cloned and cellular 16S rDNAs from a Mediterranean seawater mesocosm experiment. Image credit:''FEMS Microbiology Ecology'']]<br />
<br />
=Ecology and pathogenesis=<br />
''Moritella marina'' have been found in a variety of cold water marine environments, ranging from the ocean floor to the intestinal tract of marine organisms. Most ''Moritella'' species are thought to live mutualistically amongst larger marine organisms, but ''Moritella viscosa'' has been noted to cause skin ulcers in some fish (Urakawa, 2014). <br />
=Current Research=<br />
''Moritella marina'' has been noted for having unusually high production of the long chain polyunsaturated fatty acids (PUFAs). When ''M. marina'' strain MP-1 was cultured in medium containing cerulenin, a fatty acid synthesis inhibitor, decreases in levels of middle-chain fatty acids and remarkable increases in levels of DHA were observed. These results suggest that the synthesis of middle-chain fatty acids works independently of the synthesis of DHA. M. marina was also found to produce chitonase when induced with chiton (citation). <br />
References<br />
<br />
<br />
And, Kumar B. Kautharapu. "Kumar B. Kautharapu." ''Genome Sequence of the Psychrophilic Deep-Sea Bacterium Moritella Marina MP-1'' (ATCC 15381). N.p., 2012. Web. 02 Dec. 2016.<br />
http://jb.asm.org/content/194/22/6296.full<br />
<br />
<br />
Urakawa, H., Kita-Tsukamoto, K., Steven, S. E., Ohwada, K., & Colwell, R. R. (1998). A proposal to transfer Vibrio marinus (Russell 1891) to a new genus Moritella gen. nov. as Moritella marina comb. nov. FEMS microbiology letters, 165(2), 373-378. http://femsle.oxfordjournals.org/content/165/2/373.full#ref-13<br />
<br />
<br />
Pukall, R., Päuker, O., Buntefuß, D., Ulrichs, G., Lebaron, P., Bernard, L., ... & Stackebrandt, E. (1999). High sequence diversity of Alteromonas macleodii-related cloned and cellular 16S rDNAs from a Mediterranean seawater mesocosm experiment. FEMS Microbiology Ecology, 28(4), 335-344.<br />
http://femsec.oxfordjournals.org/content/28/4/335<br />
<br />
<br />
Stefanidi, E., & Vorgias, C. E. (2008). Molecular analysis of the gene encoding a new chitinase from the marine psychrophilic bacterium Moritella marina and biochemical characterization of the recombinant enzyme. Extremophiles, 12(4), 541-552.<br />
"FEMS Microbiology Ecology." High Sequence Diversity of Alteromonas Macleodii-related Cloned and Cellular 16S RDNAs from a Mediterranean Seawater Mesocosm Experiment | FEMS Microbiology Ecology. N.p., n.d. Web. 02 Dec. 2016.<br />
http://femsec.oxfordjournals.org/content/28/4/335 <br />
<br />
<br />
http://www.bacterio.net/moritella.html <br />
<br />
<br />
Genome structure image:<br />
https://www.patricbrc.org/portal/portal/patric/CircosGenomeViewer?cType=genome&cId=1202962.4 <br />
<br />
<br />
http://jb.asm.org/content/194/22/6296.full.pdf<br />
<br />
http://lib.dr.iastate.edu/cgi/viewcontent.cgi?article=1177&context=cbe_pubs<br />
<br />
http://www.biochemsoctrans.org/content/28/6/943.long<br />
<br />
https://www.researchgate.net/publication/232721452_Genome_Sequence_of_the_Psychrophilic_Deep-Sea_Bacterium_Moritella_marina_MP-1_ATCC_15381<br />
<br />
http://aem.asm.org/content/51/4/730.abstract?ijkey=6cfa0071e9fc15e0a007123a571561c86c1354d3&keytype2=tf_ipsecsha<br />
<br />
http://link.springer.com/referencework/10.1007%2F978-3-642-30194-0<br />
<br />
http://jb.asm.org/content/99/1/326.full.pdf <br />
<br />
<br />
Author</div>Bgalindo6106https://microbewiki.kenyon.edu/index.php?title=Moritella_marina&diff=127547Moritella marina2016-12-02T21:37:47Z<p>Bgalindo6106: /* Genome Structure */</p>
<hr />
<div>=Classification=<br />
Domain: Bacteria<br />
Phylum: Proteobacteria<br />
Class: Gammaproteobacteria<br />
Order: Alteromonadales<br />
Family: Moritellaceae<br />
Genus: Moritella<br />
<br />
<br />
=Species=<br />
''M. japonica, M. yayanosii, M. viscosa, M. profunda, M. abyssi, M. dasanesis''<br />
<br />
<br />
=Description and Significance=<br />
''Moritella marina'' is a gram-negative halophilic psychrophilic facultative anaerobe with curved or straight rods, motile polar flagella that produce PUFA’s (polyunsaturated fatty acids) and DHA (docosahexanoic acid). “Twenty-five years ago, a marine bacterium originally designated ''Vibrio marinus'' (3) and later renamed ''Moritella marina'' MP-1 (14) was reported to produce high levels of DHA (18% of the total fatty acids) (4). Such high levels of DHA presumably provide this bacterium with the ability to maintain appropriate membrane fluidity in the low temperatures and high pressures of its marine environment.”<br />
<br />
<br />
<br />
“Phylogenetic tree of some representatives of the γ-subclass of the class Proteobacteria showing the position of 16S rDNA sequences from some ''Alteromonas macleodii''-related strains and clone sequences from picoplankton DNA from the Mediterranean Sea mesocosm experiment. Sequences of clones 17B161 and 1B161 are available under accession number Y18229 and Y18231, respectively. The tree was calculated by the algorithm of De Soete [16]. The percentage of 500 bootstrap samplings that support branching points above 80% confidence are indicated. The scale bar corresponds to two nucleotide substitutions over 100 sequence positions. The tree was rooted with ''Roseobacter algicola'' as an outgroup.” (FEMS, 1998)<br />
<br />
[[image:Phytree.png|thumbnail|300px| Figure 1: The Family ''Moritellacae''(Toshi, 2014)]]<br />
<br />
=16S Ribosomal RNA Gene Information=<br />
<br />
<br />
[[image:16s.png|thumbnail|300px| Figure 2:]]<br />
<br />
“Sequence variation at positions 73–98 and 181–214 (E. coli nomenclature) within two variable regions of the 16S rDNA of representatives of clone clusters 1–5 and some strains isolated from mesocosm B. Probe target regions are in bold” (FEMS,1998)<br />
<br />
=Genome Structure=<br />
<br />
<br />
<br />
Genome structure image link:<br />
https://www.patricbrc.org/portal/portal/patric/CircosGenomeViewer?cType=genome&cId=1202962.4 <br />
Pukall, R., Päuker, O., Buntefuß, D., Ulrichs, G., Lebaron, P., Bernard, L., ... & Stackebrandt, E. (1999). High sequence diversity of Alteromonas macleodii-related cloned and cellular 16S rDNAs from a Mediterranean seawater mesocosm experiment. ''FEMS Microbiology Ecology'', 28(4), 335-344.<br />
http://femsec.oxfordjournals.org/content/28/4/335<br />
<br />
[[image:GenomeStructure.png|thumbnail|375px| Figure 3:High sequence diversity of Alteromonas macleodii-related cloned and cellular 16S rDNAs from a Mediterranean seawater mesocosm experiment. Image credit:''FEMS Microbiology Ecology'']]<br />
<br />
=Ecology and pathogenesis=<br />
''Moritella marina'' have been found in a variety of cold water marine environments, ranging from the ocean floor to the intestinal tract of marine organisms. Most ''Moritella'' species are thought to live mutualistically amongst larger marine organisms, but ''Moritella viscosa'' has been noted to cause skin ulcers in some fish (Urakawa, 2014). <br />
=Current Research=<br />
''Moritella marina'' has been noted for having unusually high production of the long chain polyunsaturated fatty acids (PUFAs). When ''M. marina'' strain MP-1 was cultured in medium containing cerulenin, a fatty acid synthesis inhibitor, decreases in levels of middle-chain fatty acids and remarkable increases in levels of DHA were observed. These results suggest that the synthesis of middle-chain fatty acids works independently of the synthesis of DHA. M. marina was also found to produce chitonase when induced with chiton (citation). <br />
References<br />
<br />
<br />
And, Kumar B. Kautharapu. "Kumar B. Kautharapu." ''Genome Sequence of the Psychrophilic Deep-Sea Bacterium Moritella Marina MP-1'' (ATCC 15381). N.p., 2012. Web. 02 Dec. 2016.<br />
http://jb.asm.org/content/194/22/6296.full<br />
<br />
<br />
Urakawa, H., Kita-Tsukamoto, K., Steven, S. E., Ohwada, K., & Colwell, R. R. (1998). A proposal to transfer Vibrio marinus (Russell 1891) to a new genus Moritella gen. nov. as Moritella marina comb. nov. FEMS microbiology letters, 165(2), 373-378. http://femsle.oxfordjournals.org/content/165/2/373.full#ref-13<br />
<br />
<br />
Pukall, R., Päuker, O., Buntefuß, D., Ulrichs, G., Lebaron, P., Bernard, L., ... & Stackebrandt, E. (1999). High sequence diversity of Alteromonas macleodii-related cloned and cellular 16S rDNAs from a Mediterranean seawater mesocosm experiment. FEMS Microbiology Ecology, 28(4), 335-344.<br />
http://femsec.oxfordjournals.org/content/28/4/335<br />
<br />
<br />
Stefanidi, E., & Vorgias, C. E. (2008). Molecular analysis of the gene encoding a new chitinase from the marine psychrophilic bacterium Moritella marina and biochemical characterization of the recombinant enzyme. Extremophiles, 12(4), 541-552.<br />
"FEMS Microbiology Ecology." High Sequence Diversity of Alteromonas Macleodii-related Cloned and Cellular 16S RDNAs from a Mediterranean Seawater Mesocosm Experiment | FEMS Microbiology Ecology. N.p., n.d. Web. 02 Dec. 2016.<br />
http://femsec.oxfordjournals.org/content/28/4/335 <br />
<br />
<br />
http://www.bacterio.net/moritella.html <br />
<br />
<br />
Genome structure image:<br />
https://www.patricbrc.org/portal/portal/patric/CircosGenomeViewer?cType=genome&cId=1202962.4 <br />
<br />
<br />
http://jb.asm.org/content/194/22/6296.full.pdf<br />
<br />
http://lib.dr.iastate.edu/cgi/viewcontent.cgi?article=1177&context=cbe_pubs<br />
<br />
http://www.biochemsoctrans.org/content/28/6/943.long<br />
<br />
https://www.researchgate.net/publication/232721452_Genome_Sequence_of_the_Psychrophilic_Deep-Sea_Bacterium_Moritella_marina_MP-1_ATCC_15381<br />
<br />
http://aem.asm.org/content/51/4/730.abstract?ijkey=6cfa0071e9fc15e0a007123a571561c86c1354d3&keytype2=tf_ipsecsha<br />
<br />
http://link.springer.com/referencework/10.1007%2F978-3-642-30194-0<br />
<br />
http://jb.asm.org/content/99/1/326.full.pdf <br />
<br />
<br />
Author</div>Bgalindo6106https://microbewiki.kenyon.edu/index.php?title=Moritella_marina&diff=127545Moritella marina2016-12-02T21:37:08Z<p>Bgalindo6106: /* Description and Significance */</p>
<hr />
<div>=Classification=<br />
Domain: Bacteria<br />
Phylum: Proteobacteria<br />
Class: Gammaproteobacteria<br />
Order: Alteromonadales<br />
Family: Moritellaceae<br />
Genus: Moritella<br />
<br />
<br />
=Species=<br />
''M. japonica, M. yayanosii, M. viscosa, M. profunda, M. abyssi, M. dasanesis''<br />
<br />
<br />
=Description and Significance=<br />
''Moritella marina'' is a gram-negative halophilic psychrophilic facultative anaerobe with curved or straight rods, motile polar flagella that produce PUFA’s (polyunsaturated fatty acids) and DHA (docosahexanoic acid). “Twenty-five years ago, a marine bacterium originally designated ''Vibrio marinus'' (3) and later renamed ''Moritella marina'' MP-1 (14) was reported to produce high levels of DHA (18% of the total fatty acids) (4). Such high levels of DHA presumably provide this bacterium with the ability to maintain appropriate membrane fluidity in the low temperatures and high pressures of its marine environment.”<br />
<br />
<br />
<br />
“Phylogenetic tree of some representatives of the γ-subclass of the class Proteobacteria showing the position of 16S rDNA sequences from some ''Alteromonas macleodii''-related strains and clone sequences from picoplankton DNA from the Mediterranean Sea mesocosm experiment. Sequences of clones 17B161 and 1B161 are available under accession number Y18229 and Y18231, respectively. The tree was calculated by the algorithm of De Soete [16]. The percentage of 500 bootstrap samplings that support branching points above 80% confidence are indicated. The scale bar corresponds to two nucleotide substitutions over 100 sequence positions. The tree was rooted with ''Roseobacter algicola'' as an outgroup.” (FEMS, 1998)<br />
<br />
[[image:Phytree.png|thumbnail|300px| Figure 1: The Family ''Moritellacae''(Toshi, 2014)]]<br />
<br />
=16S Ribosomal RNA Gene Information=<br />
<br />
<br />
[[image:16s.png|thumbnail|300px| Figure 2:]]<br />
<br />
“Sequence variation at positions 73–98 and 181–214 (E. coli nomenclature) within two variable regions of the 16S rDNA of representatives of clone clusters 1–5 and some strains isolated from mesocosm B. Probe target regions are in bold” (FEMS,1998)<br />
<br />
=Genome Structure=<br />
<br />
<br />
<br />
Genome structure image link:<br />
https://www.patricbrc.org/portal/portal/patric/CircosGenomeViewer?cType=genome&cId=1202962.4 <br />
Pukall, R., Päuker, O., Buntefuß, D., Ulrichs, G., Lebaron, P., Bernard, L., ... & Stackebrandt, E. (1999). High sequence diversity of Alteromonas macleodii-related cloned and cellular 16S rDNAs from a Mediterranean seawater mesocosm experiment. ''FEMS Microbiology Ecology'', 28(4), 335-344.<br />
http://femsec.oxfordjournals.org/content/28/4/335<br />
<br />
[[image:GenomeStructure.png|thumbnail|375px| Figure 1:High sequence diversity of Alteromonas macleodii-related cloned and cellular 16S rDNAs from a Mediterranean seawater mesocosm experiment. Image credit:''FEMS Microbiology Ecology'']]<br />
<br />
=Ecology and pathogenesis=<br />
''Moritella marina'' have been found in a variety of cold water marine environments, ranging from the ocean floor to the intestinal tract of marine organisms. Most ''Moritella'' species are thought to live mutualistically amongst larger marine organisms, but ''Moritella viscosa'' has been noted to cause skin ulcers in some fish (Urakawa, 2014). <br />
=Current Research=<br />
''Moritella marina'' has been noted for having unusually high production of the long chain polyunsaturated fatty acids (PUFAs). When ''M. marina'' strain MP-1 was cultured in medium containing cerulenin, a fatty acid synthesis inhibitor, decreases in levels of middle-chain fatty acids and remarkable increases in levels of DHA were observed. These results suggest that the synthesis of middle-chain fatty acids works independently of the synthesis of DHA. M. marina was also found to produce chitonase when induced with chiton (citation). <br />
References<br />
<br />
<br />
And, Kumar B. Kautharapu. "Kumar B. Kautharapu." ''Genome Sequence of the Psychrophilic Deep-Sea Bacterium Moritella Marina MP-1'' (ATCC 15381). N.p., 2012. Web. 02 Dec. 2016.<br />
http://jb.asm.org/content/194/22/6296.full<br />
<br />
<br />
Urakawa, H., Kita-Tsukamoto, K., Steven, S. E., Ohwada, K., & Colwell, R. R. (1998). A proposal to transfer Vibrio marinus (Russell 1891) to a new genus Moritella gen. nov. as Moritella marina comb. nov. FEMS microbiology letters, 165(2), 373-378. http://femsle.oxfordjournals.org/content/165/2/373.full#ref-13<br />
<br />
<br />
Pukall, R., Päuker, O., Buntefuß, D., Ulrichs, G., Lebaron, P., Bernard, L., ... & Stackebrandt, E. (1999). High sequence diversity of Alteromonas macleodii-related cloned and cellular 16S rDNAs from a Mediterranean seawater mesocosm experiment. FEMS Microbiology Ecology, 28(4), 335-344.<br />
http://femsec.oxfordjournals.org/content/28/4/335<br />
<br />
<br />
Stefanidi, E., & Vorgias, C. E. (2008). Molecular analysis of the gene encoding a new chitinase from the marine psychrophilic bacterium Moritella marina and biochemical characterization of the recombinant enzyme. Extremophiles, 12(4), 541-552.<br />
"FEMS Microbiology Ecology." High Sequence Diversity of Alteromonas Macleodii-related Cloned and Cellular 16S RDNAs from a Mediterranean Seawater Mesocosm Experiment | FEMS Microbiology Ecology. N.p., n.d. Web. 02 Dec. 2016.<br />
http://femsec.oxfordjournals.org/content/28/4/335 <br />
<br />
<br />
http://www.bacterio.net/moritella.html <br />
<br />
<br />
Genome structure image:<br />
https://www.patricbrc.org/portal/portal/patric/CircosGenomeViewer?cType=genome&cId=1202962.4 <br />
<br />
<br />
http://jb.asm.org/content/194/22/6296.full.pdf<br />
<br />
http://lib.dr.iastate.edu/cgi/viewcontent.cgi?article=1177&context=cbe_pubs<br />
<br />
http://www.biochemsoctrans.org/content/28/6/943.long<br />
<br />
https://www.researchgate.net/publication/232721452_Genome_Sequence_of_the_Psychrophilic_Deep-Sea_Bacterium_Moritella_marina_MP-1_ATCC_15381<br />
<br />
http://aem.asm.org/content/51/4/730.abstract?ijkey=6cfa0071e9fc15e0a007123a571561c86c1354d3&keytype2=tf_ipsecsha<br />
<br />
http://link.springer.com/referencework/10.1007%2F978-3-642-30194-0<br />
<br />
http://jb.asm.org/content/99/1/326.full.pdf <br />
<br />
<br />
Author</div>Bgalindo6106https://microbewiki.kenyon.edu/index.php?title=File:Phytree.png&diff=127543File:Phytree.png2016-12-02T21:33:27Z<p>Bgalindo6106: </p>
<hr />
<div></div>Bgalindo6106https://microbewiki.kenyon.edu/index.php?title=Moritella_marina&diff=127541Moritella marina2016-12-02T21:32:33Z<p>Bgalindo6106: /* 16S Ribosomal RNA Gene Information */</p>
<hr />
<div>=Classification=<br />
Domain: Bacteria<br />
Phylum: Proteobacteria<br />
Class: Gammaproteobacteria<br />
Order: Alteromonadales<br />
Family: Moritellaceae<br />
Genus: Moritella<br />
<br />
<br />
=Species=<br />
''M. japonica, M. yayanosii, M. viscosa, M. profunda, M. abyssi, M. dasanesis''<br />
<br />
<br />
=Description and Significance=<br />
''Moritella marina'' is a gram-negative halophilic psychrophilic facultative anaerobe with curved or straight rods, motile polar flagella that produce PUFA’s (polyunsaturated fatty acids) and DHA (docosahexanoic acid). “Twenty-five years ago, a marine bacterium originally designated ''Vibrio marinus'' (3) and later renamed ''Moritella marina'' MP-1 (14) was reported to produce high levels of DHA (18% of the total fatty acids) (4). Such high levels of DHA presumably provide this bacterium with the ability to maintain appropriate membrane fluidity in the low temperatures and high pressures of its marine environment.”<br />
<br />
<br />
<br />
“Phylogenetic tree of some representatives of the γ-subclass of the class Proteobacteria showing the position of 16S rDNA sequences from some ''Alteromonas macleodii''-related strains and clone sequences from picoplankton DNA from the Mediterranean Sea mesocosm experiment. Sequences of clones 17B161 and 1B161 are available under accession number Y18229 and Y18231, respectively. The tree was calculated by the algorithm of De Soete [16]. The percentage of 500 bootstrap samplings that support branching points above 80% confidence are indicated. The scale bar corresponds to two nucleotide substitutions over 100 sequence positions. The tree was rooted with ''Roseobacter algicola'' as an outgroup.” (FEMS, 1998)<br />
=16S Ribosomal RNA Gene Information=<br />
<br />
<br />
[[image:16s.png|thumbnail|300px| Figure 2:]]<br />
<br />
“Sequence variation at positions 73–98 and 181–214 (E. coli nomenclature) within two variable regions of the 16S rDNA of representatives of clone clusters 1–5 and some strains isolated from mesocosm B. Probe target regions are in bold” (FEMS,1998)<br />
<br />
=Genome Structure=<br />
<br />
<br />
<br />
Genome structure image link:<br />
https://www.patricbrc.org/portal/portal/patric/CircosGenomeViewer?cType=genome&cId=1202962.4 <br />
Pukall, R., Päuker, O., Buntefuß, D., Ulrichs, G., Lebaron, P., Bernard, L., ... & Stackebrandt, E. (1999). High sequence diversity of Alteromonas macleodii-related cloned and cellular 16S rDNAs from a Mediterranean seawater mesocosm experiment. ''FEMS Microbiology Ecology'', 28(4), 335-344.<br />
http://femsec.oxfordjournals.org/content/28/4/335<br />
<br />
[[image:GenomeStructure.png|thumbnail|375px| Figure 1:High sequence diversity of Alteromonas macleodii-related cloned and cellular 16S rDNAs from a Mediterranean seawater mesocosm experiment. Image credit:''FEMS Microbiology Ecology'']]<br />
<br />
=Ecology and pathogenesis=<br />
''Moritella marina'' have been found in a variety of cold water marine environments, ranging from the ocean floor to the intestinal tract of marine organisms. Most ''Moritella'' species are thought to live mutualistically amongst larger marine organisms, but ''Moritella viscosa'' has been noted to cause skin ulcers in some fish (Urakawa, 2014). <br />
=Current Research=<br />
''Moritella marina'' has been noted for having unusually high production of the long chain polyunsaturated fatty acids (PUFAs). When ''M. marina'' strain MP-1 was cultured in medium containing cerulenin, a fatty acid synthesis inhibitor, decreases in levels of middle-chain fatty acids and remarkable increases in levels of DHA were observed. These results suggest that the synthesis of middle-chain fatty acids works independently of the synthesis of DHA. M. marina was also found to produce chitonase when induced with chiton (citation). <br />
References<br />
<br />
<br />
And, Kumar B. Kautharapu. "Kumar B. Kautharapu." ''Genome Sequence of the Psychrophilic Deep-Sea Bacterium Moritella Marina MP-1'' (ATCC 15381). N.p., 2012. Web. 02 Dec. 2016.<br />
http://jb.asm.org/content/194/22/6296.full<br />
<br />
<br />
Urakawa, H., Kita-Tsukamoto, K., Steven, S. E., Ohwada, K., & Colwell, R. R. (1998). A proposal to transfer Vibrio marinus (Russell 1891) to a new genus Moritella gen. nov. as Moritella marina comb. nov. FEMS microbiology letters, 165(2), 373-378. http://femsle.oxfordjournals.org/content/165/2/373.full#ref-13<br />
<br />
<br />
Pukall, R., Päuker, O., Buntefuß, D., Ulrichs, G., Lebaron, P., Bernard, L., ... & Stackebrandt, E. (1999). High sequence diversity of Alteromonas macleodii-related cloned and cellular 16S rDNAs from a Mediterranean seawater mesocosm experiment. FEMS Microbiology Ecology, 28(4), 335-344.<br />
http://femsec.oxfordjournals.org/content/28/4/335<br />
<br />
<br />
Stefanidi, E., & Vorgias, C. E. (2008). Molecular analysis of the gene encoding a new chitinase from the marine psychrophilic bacterium Moritella marina and biochemical characterization of the recombinant enzyme. Extremophiles, 12(4), 541-552.<br />
"FEMS Microbiology Ecology." High Sequence Diversity of Alteromonas Macleodii-related Cloned and Cellular 16S RDNAs from a Mediterranean Seawater Mesocosm Experiment | FEMS Microbiology Ecology. N.p., n.d. Web. 02 Dec. 2016.<br />
http://femsec.oxfordjournals.org/content/28/4/335 <br />
<br />
<br />
http://www.bacterio.net/moritella.html <br />
<br />
<br />
Genome structure image:<br />
https://www.patricbrc.org/portal/portal/patric/CircosGenomeViewer?cType=genome&cId=1202962.4 <br />
<br />
<br />
http://jb.asm.org/content/194/22/6296.full.pdf<br />
<br />
http://lib.dr.iastate.edu/cgi/viewcontent.cgi?article=1177&context=cbe_pubs<br />
<br />
http://www.biochemsoctrans.org/content/28/6/943.long<br />
<br />
https://www.researchgate.net/publication/232721452_Genome_Sequence_of_the_Psychrophilic_Deep-Sea_Bacterium_Moritella_marina_MP-1_ATCC_15381<br />
<br />
http://aem.asm.org/content/51/4/730.abstract?ijkey=6cfa0071e9fc15e0a007123a571561c86c1354d3&keytype2=tf_ipsecsha<br />
<br />
http://link.springer.com/referencework/10.1007%2F978-3-642-30194-0<br />
<br />
http://jb.asm.org/content/99/1/326.full.pdf <br />
<br />
<br />
Author</div>Bgalindo6106https://microbewiki.kenyon.edu/index.php?title=Moritella_marina&diff=127540Moritella marina2016-12-02T21:31:36Z<p>Bgalindo6106: /* 16S Ribosomal RNA Gene Information */</p>
<hr />
<div>=Classification=<br />
Domain: Bacteria<br />
Phylum: Proteobacteria<br />
Class: Gammaproteobacteria<br />
Order: Alteromonadales<br />
Family: Moritellaceae<br />
Genus: Moritella<br />
<br />
<br />
=Species=<br />
''M. japonica, M. yayanosii, M. viscosa, M. profunda, M. abyssi, M. dasanesis''<br />
<br />
<br />
=Description and Significance=<br />
''Moritella marina'' is a gram-negative halophilic psychrophilic facultative anaerobe with curved or straight rods, motile polar flagella that produce PUFA’s (polyunsaturated fatty acids) and DHA (docosahexanoic acid). “Twenty-five years ago, a marine bacterium originally designated ''Vibrio marinus'' (3) and later renamed ''Moritella marina'' MP-1 (14) was reported to produce high levels of DHA (18% of the total fatty acids) (4). Such high levels of DHA presumably provide this bacterium with the ability to maintain appropriate membrane fluidity in the low temperatures and high pressures of its marine environment.”<br />
<br />
<br />
<br />
“Phylogenetic tree of some representatives of the γ-subclass of the class Proteobacteria showing the position of 16S rDNA sequences from some ''Alteromonas macleodii''-related strains and clone sequences from picoplankton DNA from the Mediterranean Sea mesocosm experiment. Sequences of clones 17B161 and 1B161 are available under accession number Y18229 and Y18231, respectively. The tree was calculated by the algorithm of De Soete [16]. The percentage of 500 bootstrap samplings that support branching points above 80% confidence are indicated. The scale bar corresponds to two nucleotide substitutions over 100 sequence positions. The tree was rooted with ''Roseobacter algicola'' as an outgroup.” (FEMS, 1998)<br />
=16S Ribosomal RNA Gene Information=<br />
<br />
<br />
[[image:16s.png|thumbnail|300px| Figure 2:“Sequence variation at positions 73–98 and 181–214 (E. coli nomenclature) within two variable regions of the 16S rDNA of representatives of clone clusters 1–5 and some strains isolated from mesocosm B. Probe target regions are in bold” (FEMS,1998)]]<br />
<br />
=Genome Structure=<br />
<br />
<br />
<br />
Genome structure image link:<br />
https://www.patricbrc.org/portal/portal/patric/CircosGenomeViewer?cType=genome&cId=1202962.4 <br />
Pukall, R., Päuker, O., Buntefuß, D., Ulrichs, G., Lebaron, P., Bernard, L., ... & Stackebrandt, E. (1999). High sequence diversity of Alteromonas macleodii-related cloned and cellular 16S rDNAs from a Mediterranean seawater mesocosm experiment. ''FEMS Microbiology Ecology'', 28(4), 335-344.<br />
http://femsec.oxfordjournals.org/content/28/4/335<br />
<br />
[[image:GenomeStructure.png|thumbnail|375px| Figure 1:High sequence diversity of Alteromonas macleodii-related cloned and cellular 16S rDNAs from a Mediterranean seawater mesocosm experiment. Image credit:''FEMS Microbiology Ecology'']]<br />
<br />
=Ecology and pathogenesis=<br />
''Moritella marina'' have been found in a variety of cold water marine environments, ranging from the ocean floor to the intestinal tract of marine organisms. Most ''Moritella'' species are thought to live mutualistically amongst larger marine organisms, but ''Moritella viscosa'' has been noted to cause skin ulcers in some fish (Urakawa, 2014). <br />
=Current Research=<br />
''Moritella marina'' has been noted for having unusually high production of the long chain polyunsaturated fatty acids (PUFAs). When ''M. marina'' strain MP-1 was cultured in medium containing cerulenin, a fatty acid synthesis inhibitor, decreases in levels of middle-chain fatty acids and remarkable increases in levels of DHA were observed. These results suggest that the synthesis of middle-chain fatty acids works independently of the synthesis of DHA. M. marina was also found to produce chitonase when induced with chiton (citation). <br />
References<br />
<br />
<br />
And, Kumar B. Kautharapu. "Kumar B. Kautharapu." ''Genome Sequence of the Psychrophilic Deep-Sea Bacterium Moritella Marina MP-1'' (ATCC 15381). N.p., 2012. Web. 02 Dec. 2016.<br />
http://jb.asm.org/content/194/22/6296.full<br />
<br />
<br />
Urakawa, H., Kita-Tsukamoto, K., Steven, S. E., Ohwada, K., & Colwell, R. R. (1998). A proposal to transfer Vibrio marinus (Russell 1891) to a new genus Moritella gen. nov. as Moritella marina comb. nov. FEMS microbiology letters, 165(2), 373-378. http://femsle.oxfordjournals.org/content/165/2/373.full#ref-13<br />
<br />
<br />
Pukall, R., Päuker, O., Buntefuß, D., Ulrichs, G., Lebaron, P., Bernard, L., ... & Stackebrandt, E. (1999). High sequence diversity of Alteromonas macleodii-related cloned and cellular 16S rDNAs from a Mediterranean seawater mesocosm experiment. FEMS Microbiology Ecology, 28(4), 335-344.<br />
http://femsec.oxfordjournals.org/content/28/4/335<br />
<br />
<br />
Stefanidi, E., & Vorgias, C. E. (2008). Molecular analysis of the gene encoding a new chitinase from the marine psychrophilic bacterium Moritella marina and biochemical characterization of the recombinant enzyme. Extremophiles, 12(4), 541-552.<br />
"FEMS Microbiology Ecology." High Sequence Diversity of Alteromonas Macleodii-related Cloned and Cellular 16S RDNAs from a Mediterranean Seawater Mesocosm Experiment | FEMS Microbiology Ecology. N.p., n.d. Web. 02 Dec. 2016.<br />
http://femsec.oxfordjournals.org/content/28/4/335 <br />
<br />
<br />
http://www.bacterio.net/moritella.html <br />
<br />
<br />
Genome structure image:<br />
https://www.patricbrc.org/portal/portal/patric/CircosGenomeViewer?cType=genome&cId=1202962.4 <br />
<br />
<br />
http://jb.asm.org/content/194/22/6296.full.pdf<br />
<br />
http://lib.dr.iastate.edu/cgi/viewcontent.cgi?article=1177&context=cbe_pubs<br />
<br />
http://www.biochemsoctrans.org/content/28/6/943.long<br />
<br />
https://www.researchgate.net/publication/232721452_Genome_Sequence_of_the_Psychrophilic_Deep-Sea_Bacterium_Moritella_marina_MP-1_ATCC_15381<br />
<br />
http://aem.asm.org/content/51/4/730.abstract?ijkey=6cfa0071e9fc15e0a007123a571561c86c1354d3&keytype2=tf_ipsecsha<br />
<br />
http://link.springer.com/referencework/10.1007%2F978-3-642-30194-0<br />
<br />
http://jb.asm.org/content/99/1/326.full.pdf <br />
<br />
<br />
Author</div>Bgalindo6106https://microbewiki.kenyon.edu/index.php?title=File:16s.png&diff=127534File:16s.png2016-12-02T21:28:17Z<p>Bgalindo6106: </p>
<hr />
<div></div>Bgalindo6106https://microbewiki.kenyon.edu/index.php?title=Moritella_marina&diff=127533Moritella marina2016-12-02T21:27:37Z<p>Bgalindo6106: /* Genome Structure */</p>
<hr />
<div>=Classification=<br />
Domain: Bacteria<br />
Phylum: Proteobacteria<br />
Class: Gammaproteobacteria<br />
Order: Alteromonadales<br />
Family: Moritellaceae<br />
Genus: Moritella<br />
<br />
<br />
=Species=<br />
''M. japonica, M. yayanosii, M. viscosa, M. profunda, M. abyssi, M. dasanesis''<br />
<br />
<br />
=Description and Significance=<br />
''Moritella marina'' is a gram-negative halophilic psychrophilic facultative anaerobe with curved or straight rods, motile polar flagella that produce PUFA’s (polyunsaturated fatty acids) and DHA (docosahexanoic acid). “Twenty-five years ago, a marine bacterium originally designated ''Vibrio marinus'' (3) and later renamed ''Moritella marina'' MP-1 (14) was reported to produce high levels of DHA (18% of the total fatty acids) (4). Such high levels of DHA presumably provide this bacterium with the ability to maintain appropriate membrane fluidity in the low temperatures and high pressures of its marine environment.”<br />
<br />
<br />
<br />
“Phylogenetic tree of some representatives of the γ-subclass of the class Proteobacteria showing the position of 16S rDNA sequences from some ''Alteromonas macleodii''-related strains and clone sequences from picoplankton DNA from the Mediterranean Sea mesocosm experiment. Sequences of clones 17B161 and 1B161 are available under accession number Y18229 and Y18231, respectively. The tree was calculated by the algorithm of De Soete [16]. The percentage of 500 bootstrap samplings that support branching points above 80% confidence are indicated. The scale bar corresponds to two nucleotide substitutions over 100 sequence positions. The tree was rooted with ''Roseobacter algicola'' as an outgroup.” (FEMS, 1998)<br />
=16S Ribosomal RNA Gene Information=<br />
<br />
<br />
<br />
“Sequence variation at positions 73–98 and 181–214 (E. coli nomenclature) within two variable regions of the 16S rDNA of representatives of clone clusters 1–5 and some strains isolated from mesocosm B. Probe target regions are in bold” (FEMS,1998)<br />
=Genome Structure=<br />
<br />
<br />
<br />
Genome structure image link:<br />
https://www.patricbrc.org/portal/portal/patric/CircosGenomeViewer?cType=genome&cId=1202962.4 <br />
Pukall, R., Päuker, O., Buntefuß, D., Ulrichs, G., Lebaron, P., Bernard, L., ... & Stackebrandt, E. (1999). High sequence diversity of Alteromonas macleodii-related cloned and cellular 16S rDNAs from a Mediterranean seawater mesocosm experiment. ''FEMS Microbiology Ecology'', 28(4), 335-344.<br />
http://femsec.oxfordjournals.org/content/28/4/335<br />
<br />
[[image:GenomeStructure.png|thumbnail|375px| Figure 1:High sequence diversity of Alteromonas macleodii-related cloned and cellular 16S rDNAs from a Mediterranean seawater mesocosm experiment. Image credit:''FEMS Microbiology Ecology'']]<br />
<br />
=Ecology and pathogenesis=<br />
''Moritella marina'' have been found in a variety of cold water marine environments, ranging from the ocean floor to the intestinal tract of marine organisms. Most ''Moritella'' species are thought to live mutualistically amongst larger marine organisms, but ''Moritella viscosa'' has been noted to cause skin ulcers in some fish (Urakawa, 2014). <br />
=Current Research=<br />
''Moritella marina'' has been noted for having unusually high production of the long chain polyunsaturated fatty acids (PUFAs). When ''M. marina'' strain MP-1 was cultured in medium containing cerulenin, a fatty acid synthesis inhibitor, decreases in levels of middle-chain fatty acids and remarkable increases in levels of DHA were observed. These results suggest that the synthesis of middle-chain fatty acids works independently of the synthesis of DHA. M. marina was also found to produce chitonase when induced with chiton (citation). <br />
References<br />
<br />
<br />
And, Kumar B. Kautharapu. "Kumar B. Kautharapu." ''Genome Sequence of the Psychrophilic Deep-Sea Bacterium Moritella Marina MP-1'' (ATCC 15381). N.p., 2012. Web. 02 Dec. 2016.<br />
http://jb.asm.org/content/194/22/6296.full<br />
<br />
<br />
Urakawa, H., Kita-Tsukamoto, K., Steven, S. E., Ohwada, K., & Colwell, R. R. (1998). A proposal to transfer Vibrio marinus (Russell 1891) to a new genus Moritella gen. nov. as Moritella marina comb. nov. FEMS microbiology letters, 165(2), 373-378. http://femsle.oxfordjournals.org/content/165/2/373.full#ref-13<br />
<br />
<br />
Pukall, R., Päuker, O., Buntefuß, D., Ulrichs, G., Lebaron, P., Bernard, L., ... & Stackebrandt, E. (1999). High sequence diversity of Alteromonas macleodii-related cloned and cellular 16S rDNAs from a Mediterranean seawater mesocosm experiment. FEMS Microbiology Ecology, 28(4), 335-344.<br />
http://femsec.oxfordjournals.org/content/28/4/335<br />
<br />
<br />
Stefanidi, E., & Vorgias, C. E. (2008). Molecular analysis of the gene encoding a new chitinase from the marine psychrophilic bacterium Moritella marina and biochemical characterization of the recombinant enzyme. Extremophiles, 12(4), 541-552.<br />
"FEMS Microbiology Ecology." High Sequence Diversity of Alteromonas Macleodii-related Cloned and Cellular 16S RDNAs from a Mediterranean Seawater Mesocosm Experiment | FEMS Microbiology Ecology. N.p., n.d. Web. 02 Dec. 2016.<br />
http://femsec.oxfordjournals.org/content/28/4/335 <br />
<br />
<br />
http://www.bacterio.net/moritella.html <br />
<br />
<br />
Genome structure image:<br />
https://www.patricbrc.org/portal/portal/patric/CircosGenomeViewer?cType=genome&cId=1202962.4 <br />
<br />
<br />
http://jb.asm.org/content/194/22/6296.full.pdf<br />
<br />
http://lib.dr.iastate.edu/cgi/viewcontent.cgi?article=1177&context=cbe_pubs<br />
<br />
http://www.biochemsoctrans.org/content/28/6/943.long<br />
<br />
https://www.researchgate.net/publication/232721452_Genome_Sequence_of_the_Psychrophilic_Deep-Sea_Bacterium_Moritella_marina_MP-1_ATCC_15381<br />
<br />
http://aem.asm.org/content/51/4/730.abstract?ijkey=6cfa0071e9fc15e0a007123a571561c86c1354d3&keytype2=tf_ipsecsha<br />
<br />
http://link.springer.com/referencework/10.1007%2F978-3-642-30194-0<br />
<br />
http://jb.asm.org/content/99/1/326.full.pdf <br />
<br />
<br />
Author</div>Bgalindo6106https://microbewiki.kenyon.edu/index.php?title=File:GenomeStructure.png&diff=127526File:GenomeStructure.png2016-12-02T21:24:27Z<p>Bgalindo6106: </p>
<hr />
<div></div>Bgalindo6106https://microbewiki.kenyon.edu/index.php?title=Moritella_marina&diff=127524Moritella marina2016-12-02T21:23:20Z<p>Bgalindo6106: </p>
<hr />
<div>=Classification=<br />
Domain: Bacteria<br />
Phylum: Proteobacteria<br />
Class: Gammaproteobacteria<br />
Order: Alteromonadales<br />
Family: Moritellaceae<br />
Genus: Moritella<br />
<br />
<br />
=Species=<br />
''M. japonica, M. yayanosii, M. viscosa, M. profunda, M. abyssi, M. dasanesis''<br />
<br />
<br />
=Description and Significance=<br />
''Moritella marina'' is a gram-negative halophilic psychrophilic facultative anaerobe with curved or straight rods, motile polar flagella that produce PUFA’s (polyunsaturated fatty acids) and DHA (docosahexanoic acid). “Twenty-five years ago, a marine bacterium originally designated ''Vibrio marinus'' (3) and later renamed ''Moritella marina'' MP-1 (14) was reported to produce high levels of DHA (18% of the total fatty acids) (4). Such high levels of DHA presumably provide this bacterium with the ability to maintain appropriate membrane fluidity in the low temperatures and high pressures of its marine environment.”<br />
<br />
<br />
<br />
“Phylogenetic tree of some representatives of the γ-subclass of the class Proteobacteria showing the position of 16S rDNA sequences from some ''Alteromonas macleodii''-related strains and clone sequences from picoplankton DNA from the Mediterranean Sea mesocosm experiment. Sequences of clones 17B161 and 1B161 are available under accession number Y18229 and Y18231, respectively. The tree was calculated by the algorithm of De Soete [16]. The percentage of 500 bootstrap samplings that support branching points above 80% confidence are indicated. The scale bar corresponds to two nucleotide substitutions over 100 sequence positions. The tree was rooted with ''Roseobacter algicola'' as an outgroup.” (FEMS, 1998)<br />
=16S Ribosomal RNA Gene Information=<br />
<br />
<br />
<br />
“Sequence variation at positions 73–98 and 181–214 (E. coli nomenclature) within two variable regions of the 16S rDNA of representatives of clone clusters 1–5 and some strains isolated from mesocosm B. Probe target regions are in bold” (FEMS,1998)<br />
=Genome Structure=<br />
<br />
<br />
<br />
Genome structure image link:<br />
https://www.patricbrc.org/portal/portal/patric/CircosGenomeViewer?cType=genome&cId=1202962.4 <br />
Pukall, R., Päuker, O., Buntefuß, D., Ulrichs, G., Lebaron, P., Bernard, L., ... & Stackebrandt, E. (1999). High sequence diversity of Alteromonas macleodii-related cloned and cellular 16S rDNAs from a Mediterranean seawater mesocosm experiment. ''FEMS Microbiology Ecology'', 28(4), 335-344.<br />
http://femsec.oxfordjournals.org/content/28/4/335<br />
<br />
<br />
=Ecology and pathogenesis=<br />
''Moritella marina'' have been found in a variety of cold water marine environments, ranging from the ocean floor to the intestinal tract of marine organisms. Most ''Moritella'' species are thought to live mutualistically amongst larger marine organisms, but ''Moritella viscosa'' has been noted to cause skin ulcers in some fish (Urakawa, 2014). <br />
=Current Research=<br />
''Moritella marina'' has been noted for having unusually high production of the long chain polyunsaturated fatty acids (PUFAs). When ''M. marina'' strain MP-1 was cultured in medium containing cerulenin, a fatty acid synthesis inhibitor, decreases in levels of middle-chain fatty acids and remarkable increases in levels of DHA were observed. These results suggest that the synthesis of middle-chain fatty acids works independently of the synthesis of DHA. M. marina was also found to produce chitonase when induced with chiton (citation). <br />
References<br />
<br />
<br />
And, Kumar B. Kautharapu. "Kumar B. Kautharapu." ''Genome Sequence of the Psychrophilic Deep-Sea Bacterium Moritella Marina MP-1'' (ATCC 15381). N.p., 2012. Web. 02 Dec. 2016.<br />
http://jb.asm.org/content/194/22/6296.full<br />
<br />
<br />
Urakawa, H., Kita-Tsukamoto, K., Steven, S. E., Ohwada, K., & Colwell, R. R. (1998). A proposal to transfer Vibrio marinus (Russell 1891) to a new genus Moritella gen. nov. as Moritella marina comb. nov. FEMS microbiology letters, 165(2), 373-378. http://femsle.oxfordjournals.org/content/165/2/373.full#ref-13<br />
<br />
<br />
Pukall, R., Päuker, O., Buntefuß, D., Ulrichs, G., Lebaron, P., Bernard, L., ... & Stackebrandt, E. (1999). High sequence diversity of Alteromonas macleodii-related cloned and cellular 16S rDNAs from a Mediterranean seawater mesocosm experiment. FEMS Microbiology Ecology, 28(4), 335-344.<br />
http://femsec.oxfordjournals.org/content/28/4/335<br />
<br />
<br />
Stefanidi, E., & Vorgias, C. E. (2008). Molecular analysis of the gene encoding a new chitinase from the marine psychrophilic bacterium Moritella marina and biochemical characterization of the recombinant enzyme. Extremophiles, 12(4), 541-552.<br />
"FEMS Microbiology Ecology." High Sequence Diversity of Alteromonas Macleodii-related Cloned and Cellular 16S RDNAs from a Mediterranean Seawater Mesocosm Experiment | FEMS Microbiology Ecology. N.p., n.d. Web. 02 Dec. 2016.<br />
http://femsec.oxfordjournals.org/content/28/4/335 <br />
<br />
<br />
http://www.bacterio.net/moritella.html <br />
<br />
<br />
Genome structure image:<br />
https://www.patricbrc.org/portal/portal/patric/CircosGenomeViewer?cType=genome&cId=1202962.4 <br />
<br />
<br />
http://jb.asm.org/content/194/22/6296.full.pdf<br />
<br />
http://lib.dr.iastate.edu/cgi/viewcontent.cgi?article=1177&context=cbe_pubs<br />
<br />
http://www.biochemsoctrans.org/content/28/6/943.long<br />
<br />
https://www.researchgate.net/publication/232721452_Genome_Sequence_of_the_Psychrophilic_Deep-Sea_Bacterium_Moritella_marina_MP-1_ATCC_15381<br />
<br />
http://aem.asm.org/content/51/4/730.abstract?ijkey=6cfa0071e9fc15e0a007123a571561c86c1354d3&keytype2=tf_ipsecsha<br />
<br />
http://link.springer.com/referencework/10.1007%2F978-3-642-30194-0<br />
<br />
http://jb.asm.org/content/99/1/326.full.pdf <br />
<br />
<br />
Author</div>Bgalindo6106https://microbewiki.kenyon.edu/index.php?title=Moritella_marina&diff=127517Moritella marina2016-12-02T21:18:32Z<p>Bgalindo6106: </p>
<hr />
<div>=Classification=<br />
Domain: Bacteria<br />
Phylum: Proteobacteria<br />
Class: Gammaproteobacteria<br />
Order: Alteromonadales<br />
Family: Moritellaceae<br />
Genus: Moritella<br />
<br />
<br />
=Species=<br />
''M. japonica, M. yayanosii, M. viscosa, M. profunda, M. abyssi, M. dasanesis''<br />
<br />
<br />
=Description and Significance=<br />
''Moritella marina'' is a gram-negative halophilic psychrophilic facultative anaerobe with curved or straight rods, motile polar flagella that produce PUFA’s (polyunsaturated fatty acids) and DHA (docosahexanoic acid). “Twenty-five years ago, a marine bacterium originally designated ''Vibrio marinus'' (3) and later renamed ''Moritella marina'' MP-1 (14) was reported to produce high levels of DHA (18% of the total fatty acids) (4). Such high levels of DHA presumably provide this bacterium with the ability to maintain appropriate membrane fluidity in the low temperatures and high pressures of its marine environment.”<br />
<br />
<br />
<br />
“Phylogenetic tree of some representatives of the γ-subclass of the class Proteobacteria showing the position of 16S rDNA sequences from some ''Alteromonas macleodii''-related strains and clone sequences from picoplankton DNA from the Mediterranean Sea mesocosm experiment. Sequences of clones 17B161 and 1B161 are available under accession number Y18229 and Y18231, respectively. The tree was calculated by the algorithm of De Soete [16]. The percentage of 500 bootstrap samplings that support branching points above 80% confidence are indicated. The scale bar corresponds to two nucleotide substitutions over 100 sequence positions. The tree was rooted with ''Roseobacter algicola'' as an outgroup.” (FEMS, 1998)<br />
=16S Ribosomal RNA Gene Information=<br />
<br />
<br />
<br />
“Sequence variation at positions 73–98 and 181–214 (E. coli nomenclature) within two variable regions of the 16S rDNA of representatives of clone clusters 1–5 and some strains isolated from mesocosm B. Probe target regions are in bold” (FEMS,1998)<br />
=Genome Structure=<br />
<br />
<br />
<br />
Genome structure image link:<br />
https://www.patricbrc.org/portal/portal/patric/CircosGenomeViewer?cType=genome&cId=1202962.4 <br />
Pukall, R., Päuker, O., Buntefuß, D., Ulrichs, G., Lebaron, P., Bernard, L., ... & Stackebrandt, E. (1999). High sequence diversity of Alteromonas macleodii-related cloned and cellular 16S rDNAs from a Mediterranean seawater mesocosm experiment. ''FEMS Microbiology Ecology'', 28(4), 335-344.<br />
http://femsec.oxfordjournals.org/content/28/4/335<br />
<br />
<br />
=Ecology and pathogenesis=<br />
''Moritella marina'' have been found in a variety of cold water marine environments, ranging from the ocean floor to the intestinal tract of marine organisms. Most ''Moritella'' species are thought to live mutualistically amongst larger marine organisms, but ''Moritella viscosa'' has been noted to cause skin ulcers in some fish (Urakawa, 2014). <br />
=Current Research=<br />
''Moritella marina'' has been noted for having unusually high production of the long chain polyunsaturated fatty acids (PUFAs). When ''M. marina'' strain MP-1 was cultured in medium containing cerulenin, a fatty acid synthesis inhibitor, decreases in levels of middle-chain fatty acids and remarkable increases in levels of DHA were observed. These results suggest that the synthesis of middle-chain fatty acids works independently of the synthesis of DHA. M. marina was also found to produce chitonase when induced with chiton (citation). <br />
References<br />
<br />
<br />
And, Kumar B. Kautharapu. "Kumar B. Kautharapu." ''Genome Sequence of the Psychrophilic Deep-Sea Bacterium Moritella Marina MP-1'' (ATCC 15381). N.p., 2012. Web. 02 Dec. 2016.<br />
http://jb.asm.org/content/194/22/6296.full<br />
<br />
<br />
Urakawa, H., Kita-Tsukamoto, K., Steven, S. E., Ohwada, K., & Colwell, R. R. (1998). A proposal to transfer Vibrio marinus (Russell 1891) to a new genus Moritella gen. nov. as Moritella marina comb. nov. FEMS microbiology letters, 165(2), 373-378. http://femsle.oxfordjournals.org/content/165/2/373.full#ref-13<br />
<br />
<br />
Pukall, R., Päuker, O., Buntefuß, D., Ulrichs, G., Lebaron, P., Bernard, L., ... & Stackebrandt, E. (1999). High sequence diversity of Alteromonas macleodii-related cloned and cellular 16S rDNAs from a Mediterranean seawater mesocosm experiment. FEMS Microbiology Ecology, 28(4), 335-344.<br />
http://femsec.oxfordjournals.org/content/28/4/335<br />
<br />
<br />
Stefanidi, E., & Vorgias, C. E. (2008). Molecular analysis of the gene encoding a new chitinase from the marine psychrophilic bacterium Moritella marina and biochemical characterization of the recombinant enzyme. Extremophiles, 12(4), 541-552.<br />
"FEMS Microbiology Ecology." High Sequence Diversity of Alteromonas Macleodii-related Cloned and Cellular 16S RDNAs from a Mediterranean Seawater Mesocosm Experiment | FEMS Microbiology Ecology. N.p., n.d. Web. 02 Dec. 2016.<br />
http://femsec.oxfordjournals.org/content/28/4/335 <br />
<br />
<br />
http://www.bacterio.net/moritella.html <br />
<br />
<br />
Genome structure image:<br />
https://www.patricbrc.org/portal/portal/patric/CircosGenomeViewer?cType=genome&cId=1202962.4 <br />
<br />
<br />
http://jb.asm.org/content/194/22/6296.full.pdf<br />
http://lib.dr.iastate.edu/cgi/viewcontent.cgi?article=1177&context=cbe_pubs<br />
http://www.biochemsoctrans.org/content/28/6/943.long<br />
https://www.researchgate.net/publication/232721452_Genome_Sequence_of_the_Psychrophilic_Deep-Sea_Bacterium_Moritella_marina_MP-1_ATCC_15381<br />
http://aem.asm.org/content/51/4/730.abstract?ijkey=6cfa0071e9fc15e0a007123a571561c86c1354d3&keytype2=tf_ipsecsha<br />
http://link.springer.com/referencework/10.1007%2F978-3-642-30194-0<br />
http://jb.asm.org/content/99/1/326.full.pdf <br />
<br />
<br />
Author</div>Bgalindo6106https://microbewiki.kenyon.edu/index.php?title=Moritella_marina&diff=127514Moritella marina2016-12-02T21:14:51Z<p>Bgalindo6106: </p>
<hr />
<div><br />
=Classification=<br />
Domain: Bacteria<br />
Phylum: Proteobacteria<br />
Class: Gammaproteobacteria<br />
Order: Alteromonadales<br />
Family: Moritellaceae<br />
Genus: Moritella<br />
<br />
<br />
=Species=<br />
''M. japonica, M. yayanosii, M. viscosa, M. profunda, M. abyssi, M. dasanesis''<br />
<br />
<br />
=Description and Significance=<br />
''Moritella marina'' is a gram-negative halophilic psychrophilic facultative anaerobe with curved or straight rods, motile polar flagella that produce PUFA’s (polyunsaturated fatty acids) and DHA (docosahexanoic acid). “Twenty-five years ago, a marine bacterium originally designated ''Vibrio marinus'' (3) and later renamed ''Moritella marina'' MP-1 (14) was reported to produce high levels of DHA (18% of the total fatty acids) (4). Such high levels of DHA presumably provide this bacterium with the ability to maintain appropriate membrane fluidity in the low temperatures and high pressures of its marine environment.”<br />
<br />
<br />
<br />
“Phylogenetic tree of some representatives of the γ-subclass of the class Proteobacteria showing the position of 16S rDNA sequences from some ''Alteromonas macleodii''-related strains and clone sequences from picoplankton DNA from the Mediterranean Sea mesocosm experiment. Sequences of clones 17B161 and 1B161 are available under accession number Y18229 and Y18231, respectively. The tree was calculated by the algorithm of De Soete [16]. The percentage of 500 bootstrap samplings that support branching points above 80% confidence are indicated. The scale bar corresponds to two nucleotide substitutions over 100 sequence positions. The tree was rooted with ''Roseobacter algicola'' as an outgroup.” (FEMS, 1998)<br />
=16S Ribosomal RNA Gene Information=<br />
<br />
<br />
<br />
“Sequence variation at positions 73–98 and 181–214 (E. coli nomenclature) within two variable regions of the 16S rDNA of representatives of clone clusters 1–5 and some strains isolated from mesocosm B. Probe target regions are in bold” (FEMS,1998)<br />
=Genome Structure=<br />
<br />
<br />
<br />
Genome structure image link:<br />
https://www.patricbrc.org/portal/portal/patric/CircosGenomeViewer?cType=genome&cId=1202962.4 <br />
Pukall, R., Päuker, O., Buntefuß, D., Ulrichs, G., Lebaron, P., Bernard, L., ... & Stackebrandt, E. (1999). High sequence diversity of Alteromonas macleodii-related cloned and cellular 16S rDNAs from a Mediterranean seawater mesocosm experiment. ''FEMS Microbiology Ecology'', 28(4), 335-344.<br />
http://femsec.oxfordjournals.org/content/28/4/335<br />
<br />
<br />
=Ecology and pathogenesis=<br />
''Moritella marina'' have been found in a variety of cold water marine environments, ranging from the ocean floor to the intestinal tract of marine organisms. Most ''Moritella'' species are thought to live mutualistically amongst larger marine organisms, but ''Moritella viscosa'' has been noted to cause skin ulcers in some fish (Urakawa, 2014). <br />
=Current Research=<br />
''Moritella marina'' has been noted for having unusually high production of the long chain polyunsaturated fatty acids (PUFAs). When ''M. marina'' strain MP-1 was cultured in medium containing cerulenin, a fatty acid synthesis inhibitor, decreases in levels of middle-chain fatty acids and remarkable increases in levels of DHA were observed. These results suggest that the synthesis of middle-chain fatty acids works independently of the synthesis of DHA. M. marina was also found to produce chitonase when induced with chiton (citation). <br />
References<br />
<br />
<br />
And, Kumar B. Kautharapu. "Kumar B. Kautharapu." ''Genome Sequence of the Psychrophilic Deep-Sea Bacterium Moritella Marina MP-1'' (ATCC 15381). N.p., 2012. Web. 02 Dec. 2016.<br />
http://jb.asm.org/content/194/22/6296.full<br />
<br />
<br />
Urakawa, H., Kita-Tsukamoto, K., Steven, S. E., Ohwada, K., & Colwell, R. R. (1998). A proposal to transfer Vibrio marinus (Russell 1891) to a new genus Moritella gen. nov. as Moritella marina comb. nov. FEMS microbiology letters, 165(2), 373-378. http://femsle.oxfordjournals.org/content/165/2/373.full#ref-13<br />
<br />
<br />
Pukall, R., Päuker, O., Buntefuß, D., Ulrichs, G., Lebaron, P., Bernard, L., ... & Stackebrandt, E. (1999). High sequence diversity of Alteromonas macleodii-related cloned and cellular 16S rDNAs from a Mediterranean seawater mesocosm experiment. FEMS Microbiology Ecology, 28(4), 335-344.<br />
http://femsec.oxfordjournals.org/content/28/4/335<br />
<br />
<br />
Stefanidi, E., & Vorgias, C. E. (2008). Molecular analysis of the gene encoding a new chitinase from the marine psychrophilic bacterium Moritella marina and biochemical characterization of the recombinant enzyme. Extremophiles, 12(4), 541-552.<br />
"FEMS Microbiology Ecology." High Sequence Diversity of Alteromonas Macleodii-related Cloned and Cellular 16S RDNAs from a Mediterranean Seawater Mesocosm Experiment | FEMS Microbiology Ecology. N.p., n.d. Web. 02 Dec. 2016.<br />
http://femsec.oxfordjournals.org/content/28/4/335 <br />
<br />
<br />
http://www.bacterio.net/moritella.html <br />
<br />
<br />
Genome structure image:<br />
https://www.patricbrc.org/portal/portal/patric/CircosGenomeViewer?cType=genome&cId=1202962.4 <br />
<br />
<br />
http://jb.asm.org/content/194/22/6296.full.pdf<br />
http://lib.dr.iastate.edu/cgi/viewcontent.cgi?article=1177&context=cbe_pubs<br />
http://www.biochemsoctrans.org/content/28/6/943.long<br />
https://www.researchgate.net/publication/232721452_Genome_Sequence_of_the_Psychrophilic_Deep-Sea_Bacterium_Moritella_marina_MP-1_ATCC_15381<br />
http://aem.asm.org/content/51/4/730.abstract?ijkey=6cfa0071e9fc15e0a007123a571561c86c1354d3&keytype2=tf_ipsecsha<br />
http://link.springer.com/referencework/10.1007%2F978-3-642-30194-0<br />
http://jb.asm.org/content/99/1/326.full.pdf <br />
<br />
<br />
Author</div>Bgalindo6106https://microbewiki.kenyon.edu/index.php?title=Moritella_marina&diff=127512Moritella marina2016-12-02T21:14:26Z<p>Bgalindo6106: </p>
<hr />
<div>''Moritella Marina''<br />
<br />
Higher order taxa<br />
<br />
=Classification=<br />
Domain: Bacteria<br />
Phylum: Proteobacteria<br />
Class: Gammaproteobacteria<br />
Order: Alteromonadales<br />
Family: Moritellaceae<br />
Genus: Moritella<br />
<br />
<br />
=Species=<br />
''M. japonica, M. yayanosii, M. viscosa, M. profunda, M. abyssi, M. dasanesis''<br />
<br />
<br />
=Description and Significance=<br />
''Moritella marina'' is a gram-negative halophilic psychrophilic facultative anaerobe with curved or straight rods, motile polar flagella that produce PUFA’s (polyunsaturated fatty acids) and DHA (docosahexanoic acid). “Twenty-five years ago, a marine bacterium originally designated ''Vibrio marinus'' (3) and later renamed ''Moritella marina'' MP-1 (14) was reported to produce high levels of DHA (18% of the total fatty acids) (4). Such high levels of DHA presumably provide this bacterium with the ability to maintain appropriate membrane fluidity in the low temperatures and high pressures of its marine environment.”<br />
<br />
<br />
<br />
“Phylogenetic tree of some representatives of the γ-subclass of the class Proteobacteria showing the position of 16S rDNA sequences from some ''Alteromonas macleodii''-related strains and clone sequences from picoplankton DNA from the Mediterranean Sea mesocosm experiment. Sequences of clones 17B161 and 1B161 are available under accession number Y18229 and Y18231, respectively. The tree was calculated by the algorithm of De Soete [16]. The percentage of 500 bootstrap samplings that support branching points above 80% confidence are indicated. The scale bar corresponds to two nucleotide substitutions over 100 sequence positions. The tree was rooted with ''Roseobacter algicola'' as an outgroup.” (FEMS, 1998)<br />
=16S Ribosomal RNA Gene Information=<br />
<br />
<br />
<br />
“Sequence variation at positions 73–98 and 181–214 (E. coli nomenclature) within two variable regions of the 16S rDNA of representatives of clone clusters 1–5 and some strains isolated from mesocosm B. Probe target regions are in bold” (FEMS,1998)<br />
=Genome Structure=<br />
<br />
<br />
<br />
Genome structure image link:<br />
https://www.patricbrc.org/portal/portal/patric/CircosGenomeViewer?cType=genome&cId=1202962.4 <br />
Pukall, R., Päuker, O., Buntefuß, D., Ulrichs, G., Lebaron, P., Bernard, L., ... & Stackebrandt, E. (1999). High sequence diversity of Alteromonas macleodii-related cloned and cellular 16S rDNAs from a Mediterranean seawater mesocosm experiment. ''FEMS Microbiology Ecology'', 28(4), 335-344.<br />
http://femsec.oxfordjournals.org/content/28/4/335<br />
<br />
<br />
=Ecology and pathogenesis=<br />
''Moritella marina'' have been found in a variety of cold water marine environments, ranging from the ocean floor to the intestinal tract of marine organisms. Most ''Moritella'' species are thought to live mutualistically amongst larger marine organisms, but ''Moritella viscosa'' has been noted to cause skin ulcers in some fish (Urakawa, 2014). <br />
=Current Research=<br />
''Moritella marina'' has been noted for having unusually high production of the long chain polyunsaturated fatty acids (PUFAs). When ''M. marina'' strain MP-1 was cultured in medium containing cerulenin, a fatty acid synthesis inhibitor, decreases in levels of middle-chain fatty acids and remarkable increases in levels of DHA were observed. These results suggest that the synthesis of middle-chain fatty acids works independently of the synthesis of DHA. M. marina was also found to produce chitonase when induced with chiton (citation). <br />
References<br />
<br />
<br />
And, Kumar B. Kautharapu. "Kumar B. Kautharapu." ''Genome Sequence of the Psychrophilic Deep-Sea Bacterium Moritella Marina MP-1'' (ATCC 15381). N.p., 2012. Web. 02 Dec. 2016.<br />
http://jb.asm.org/content/194/22/6296.full<br />
<br />
<br />
Urakawa, H., Kita-Tsukamoto, K., Steven, S. E., Ohwada, K., & Colwell, R. R. (1998). A proposal to transfer Vibrio marinus (Russell 1891) to a new genus Moritella gen. nov. as Moritella marina comb. nov. FEMS microbiology letters, 165(2), 373-378. http://femsle.oxfordjournals.org/content/165/2/373.full#ref-13<br />
<br />
<br />
Pukall, R., Päuker, O., Buntefuß, D., Ulrichs, G., Lebaron, P., Bernard, L., ... & Stackebrandt, E. (1999). High sequence diversity of Alteromonas macleodii-related cloned and cellular 16S rDNAs from a Mediterranean seawater mesocosm experiment. FEMS Microbiology Ecology, 28(4), 335-344.<br />
http://femsec.oxfordjournals.org/content/28/4/335<br />
<br />
<br />
Stefanidi, E., & Vorgias, C. E. (2008). Molecular analysis of the gene encoding a new chitinase from the marine psychrophilic bacterium Moritella marina and biochemical characterization of the recombinant enzyme. Extremophiles, 12(4), 541-552.<br />
"FEMS Microbiology Ecology." High Sequence Diversity of Alteromonas Macleodii-related Cloned and Cellular 16S RDNAs from a Mediterranean Seawater Mesocosm Experiment | FEMS Microbiology Ecology. N.p., n.d. Web. 02 Dec. 2016.<br />
http://femsec.oxfordjournals.org/content/28/4/335 <br />
<br />
<br />
http://www.bacterio.net/moritella.html <br />
<br />
<br />
Genome structure image:<br />
https://www.patricbrc.org/portal/portal/patric/CircosGenomeViewer?cType=genome&cId=1202962.4 <br />
<br />
<br />
http://jb.asm.org/content/194/22/6296.full.pdf<br />
http://lib.dr.iastate.edu/cgi/viewcontent.cgi?article=1177&context=cbe_pubs<br />
http://www.biochemsoctrans.org/content/28/6/943.long<br />
https://www.researchgate.net/publication/232721452_Genome_Sequence_of_the_Psychrophilic_Deep-Sea_Bacterium_Moritella_marina_MP-1_ATCC_15381<br />
http://aem.asm.org/content/51/4/730.abstract?ijkey=6cfa0071e9fc15e0a007123a571561c86c1354d3&keytype2=tf_ipsecsha<br />
http://link.springer.com/referencework/10.1007%2F978-3-642-30194-0<br />
http://jb.asm.org/content/99/1/326.full.pdf <br />
<br />
<br />
Author</div>Bgalindo6106https://microbewiki.kenyon.edu/index.php?title=Moritella_marina&diff=127507Moritella marina2016-12-02T21:10:51Z<p>Bgalindo6106: </p>
<hr />
<div>''Moritella Marina''<br />
Higher order taxa<br />
=Classification=<br />
Domain: Bacteria<br />
Phylum: Proteobacteria<br />
Class: Gammaproteobacteria<br />
Order: Alteromonadales<br />
Family: Moritellaceae<br />
Genus: Moritella<br />
<br />
<br />
=Species=<br />
''M. japonica, M. yayanosii, M. viscosa, M. profunda, M. abyssi, M. dasanesis''<br />
<br />
<br />
=Description and Significance=<br />
''Moritella marina'' is a gram-negative halophilic psychrophilic facultative anaerobe with curved or straight rods, motile polar flagella that produce PUFA’s (polyunsaturated fatty acids) and DHA (docosahexanoic acid). “Twenty-five years ago, a marine bacterium originally designated ''Vibrio marinus'' (3) and later renamed ''Moritella marina'' MP-1 (14) was reported to produce high levels of DHA (18% of the total fatty acids) (4). Such high levels of DHA presumably provide this bacterium with the ability to maintain appropriate membrane fluidity in the low temperatures and high pressures of its marine environment.”<br />
<br />
<br />
<br />
“Phylogenetic tree of some representatives of the γ-subclass of the class Proteobacteria showing the position of 16S rDNA sequences from some ''Alteromonas macleodii''-related strains and clone sequences from picoplankton DNA from the Mediterranean Sea mesocosm experiment. Sequences of clones 17B161 and 1B161 are available under accession number Y18229 and Y18231, respectively. The tree was calculated by the algorithm of De Soete [16]. The percentage of 500 bootstrap samplings that support branching points above 80% confidence are indicated. The scale bar corresponds to two nucleotide substitutions over 100 sequence positions. The tree was rooted with ''Roseobacter algicola'' as an outgroup.” (FEMS, 1998)<br />
=16S Ribosomal RNA Gene Information=<br />
<br />
<br />
<br />
“Sequence variation at positions 73–98 and 181–214 (E. coli nomenclature) within two variable regions of the 16S rDNA of representatives of clone clusters 1–5 and some strains isolated from mesocosm B. Probe target regions are in bold” (FEMS,1998)<br />
=Genome Structure=<br />
<br />
<br />
<br />
Genome structure image link:<br />
https://www.patricbrc.org/portal/portal/patric/CircosGenomeViewer?cType=genome&cId=1202962.4 <br />
Pukall, R., Päuker, O., Buntefuß, D., Ulrichs, G., Lebaron, P., Bernard, L., ... & Stackebrandt, E. (1999). High sequence diversity of Alteromonas macleodii-related cloned and cellular 16S rDNAs from a Mediterranean seawater mesocosm experiment. ''FEMS Microbiology Ecology'', 28(4), 335-344.<br />
http://femsec.oxfordjournals.org/content/28/4/335<br />
<br />
<br />
=Ecology and pathogenesis=<br />
''Moritella marina'' have been found in a variety of cold water marine environments, ranging from the ocean floor to the intestinal tract of marine organisms. Most ''Moritella'' species are thought to live mutualistically amongst larger marine organisms, but ''Moritella viscosa'' has been noted to cause skin ulcers in some fish (Urakawa, 2014). <br />
=Current Research=<br />
''Moritella marina'' has been noted for having unusually high production of the long chain polyunsaturated fatty acids (PUFAs). When ''M. marina'' strain MP-1 was cultured in medium containing cerulenin, a fatty acid synthesis inhibitor, decreases in levels of middle-chain fatty acids and remarkable increases in levels of DHA were observed. These results suggest that the synthesis of middle-chain fatty acids works independently of the synthesis of DHA. M. marina was also found to produce chitonase when induced with chiton (citation). <br />
References<br />
<br />
<br />
And, Kumar B. Kautharapu. "Kumar B. Kautharapu." ''Genome Sequence of the Psychrophilic Deep-Sea Bacterium Moritella Marina MP-1'' (ATCC 15381). N.p., 2012. Web. 02 Dec. 2016.<br />
http://jb.asm.org/content/194/22/6296.full<br />
<br />
<br />
Urakawa, H., Kita-Tsukamoto, K., Steven, S. E., Ohwada, K., & Colwell, R. R. (1998). A proposal to transfer Vibrio marinus (Russell 1891) to a new genus Moritella gen. nov. as Moritella marina comb. nov. FEMS microbiology letters, 165(2), 373-378. http://femsle.oxfordjournals.org/content/165/2/373.full#ref-13<br />
<br />
<br />
Pukall, R., Päuker, O., Buntefuß, D., Ulrichs, G., Lebaron, P., Bernard, L., ... & Stackebrandt, E. (1999). High sequence diversity of Alteromonas macleodii-related cloned and cellular 16S rDNAs from a Mediterranean seawater mesocosm experiment. FEMS Microbiology Ecology, 28(4), 335-344.<br />
http://femsec.oxfordjournals.org/content/28/4/335<br />
<br />
<br />
Stefanidi, E., & Vorgias, C. E. (2008). Molecular analysis of the gene encoding a new chitinase from the marine psychrophilic bacterium Moritella marina and biochemical characterization of the recombinant enzyme. Extremophiles, 12(4), 541-552.<br />
"FEMS Microbiology Ecology." High Sequence Diversity of Alteromonas Macleodii-related Cloned and Cellular 16S RDNAs from a Mediterranean Seawater Mesocosm Experiment | FEMS Microbiology Ecology. N.p., n.d. Web. 02 Dec. 2016.<br />
http://femsec.oxfordjournals.org/content/28/4/335 <br />
<br />
<br />
http://www.bacterio.net/moritella.html <br />
<br />
<br />
Genome structure image:<br />
https://www.patricbrc.org/portal/portal/patric/CircosGenomeViewer?cType=genome&cId=1202962.4 <br />
<br />
<br />
http://jb.asm.org/content/194/22/6296.full.pdf<br />
http://lib.dr.iastate.edu/cgi/viewcontent.cgi?article=1177&context=cbe_pubs<br />
http://www.biochemsoctrans.org/content/28/6/943.long<br />
https://www.researchgate.net/publication/232721452_Genome_Sequence_of_the_Psychrophilic_Deep-Sea_Bacterium_Moritella_marina_MP-1_ATCC_15381<br />
http://aem.asm.org/content/51/4/730.abstract?ijkey=6cfa0071e9fc15e0a007123a571561c86c1354d3&keytype2=tf_ipsecsha<br />
http://link.springer.com/referencework/10.1007%2F978-3-642-30194-0<br />
http://jb.asm.org/content/99/1/326.full.pdf <br />
<br />
<br />
Author</div>Bgalindo6106https://microbewiki.kenyon.edu/index.php?title=Moritella_marina&diff=127505Moritella marina2016-12-02T21:09:02Z<p>Bgalindo6106: </p>
<hr />
<div>''Moritella Marina''<br />
Higher order taxa<br />
=Classification=<br />
Domain: Bacteria<br />
Phylum: Proteobacteria<br />
Class: Gammaproteobacteria<br />
Order: Alteromonadales<br />
Family: Moritellaceae<br />
Genus: Moritella<br />
<br />
<br />
=Species=<br />
''M. japonica, M. yayanosii, M. viscosa, M. profunda, M. abyssi, M. dasanesis''<br />
<br />
<br />
=Description and Significance=<br />
''Moritella marina'' is a gram-negative halophilic psychrophilic facultative anaerobe with curved or straight rods, motile polar flagella that produce PUFA’s (polyunsaturated fatty acids) and DHA (docosahexanoic acid). “Twenty-five years ago, a marine bacterium originally designated ''Vibrio marinus'' (3) and later renamed ''Moritella marina'' MP-1 (14) was reported to produce high levels of DHA (18% of the total fatty acids) (4). Such high levels of DHA presumably provide this bacterium with the ability to maintain appropriate membrane fluidity in the low temperatures and high pressures of its marine environment.”<br />
<br />
<br />
<br />
“Phylogenetic tree of some representatives of the γ-subclass of the class Proteobacteria showing the position of 16S rDNA sequences from some ''Alteromonas macleodii''-related strains and clone sequences from picoplankton DNA from the Mediterranean Sea mesocosm experiment. Sequences of clones 17B161 and 1B161 are available under accession number Y18229 and Y18231, respectively. The tree was calculated by the algorithm of De Soete [16]. The percentage of 500 bootstrap samplings that support branching points above 80% confidence are indicated. The scale bar corresponds to two nucleotide substitutions over 100 sequence positions. The tree was rooted with ''Roseobacter algicola'' as an outgroup.” (FEMS, 1998)<br />
=16S Ribosomal RNA Gene Information=<br />
<br />
<br />
<br />
“Sequence variation at positions 73–98 and 181–214 (E. coli nomenclature) within two variable regions of the 16S rDNA of representatives of clone clusters 1–5 and some strains isolated from mesocosm B. Probe target regions are in bold” (FEMS,1998)<br />
=Genome Structure=<br />
<br />
<br />
<br />
Genome structure image link:<br />
https://www.patricbrc.org/portal/portal/patric/CircosGenomeViewer?cType=genome&cId=1202962.4 <br />
Pukall, R., Päuker, O., Buntefuß, D., Ulrichs, G., Lebaron, P., Bernard, L., ... & Stackebrandt, E. (1999). High sequence diversity of Alteromonas macleodii-related cloned and cellular 16S rDNAs from a Mediterranean seawater mesocosm experiment. FEMS Microbiology Ecology, 28(4), 335-344.<br />
http://femsec.oxfordjournals.org/content/28/4/335<br />
<br />
<br />
=Ecology and pathogenesis=<br />
Moritella marina have been found in a variety of cold water marine environments, ranging from the ocean floor to the intestinal tract of marine organisms. Most Moritella species are thought to live mutualistically amongst larger marine organisms, but Moritella viscosa has been noted to cause skin ulcers in some fish (Urakawa, 2014). <br />
=Current Research=<br />
Moritella marina has been noted for having unusually high production of the long chain polyunsaturated fatty acids (PUFAs). When M. marina strain MP-1 was cultured in medium containing cerulenin, a fatty acid synthesis inhibitor, decreases in levels of middle-chain fatty acids and remarkable increases in levels of DHA were observed. These results suggest that the synthesis of middle-chain fatty acids works independently of the synthesis of DHA. M. marina was also found to produce chitonase when induced with chiton (citation). <br />
References<br />
<br />
<br />
And, Kumar B. Kautharapu. "Kumar B. Kautharapu." Genome Sequence of the Psychrophilic Deep-Sea Bacterium Moritella Marina MP-1 (ATCC 15381). N.p., 2012. Web. 02 Dec. 2016.<br />
http://jb.asm.org/content/194/22/6296.full<br />
<br />
<br />
Urakawa, H., Kita-Tsukamoto, K., Steven, S. E., Ohwada, K., & Colwell, R. R. (1998). A proposal to transfer Vibrio marinus (Russell 1891) to a new genus Moritella gen. nov. as Moritella marina comb. nov. FEMS microbiology letters, 165(2), 373-378. http://femsle.oxfordjournals.org/content/165/2/373.full#ref-13<br />
<br />
<br />
Pukall, R., Päuker, O., Buntefuß, D., Ulrichs, G., Lebaron, P., Bernard, L., ... & Stackebrandt, E. (1999). High sequence diversity of Alteromonas macleodii-related cloned and cellular 16S rDNAs from a Mediterranean seawater mesocosm experiment. FEMS Microbiology Ecology, 28(4), 335-344.<br />
http://femsec.oxfordjournals.org/content/28/4/335<br />
<br />
<br />
Stefanidi, E., & Vorgias, C. E. (2008). Molecular analysis of the gene encoding a new chitinase from the marine psychrophilic bacterium Moritella marina and biochemical characterization of the recombinant enzyme. Extremophiles, 12(4), 541-552.<br />
"FEMS Microbiology Ecology." High Sequence Diversity of Alteromonas Macleodii-related Cloned and Cellular 16S RDNAs from a Mediterranean Seawater Mesocosm Experiment | FEMS Microbiology Ecology. N.p., n.d. Web. 02 Dec. 2016.<br />
http://femsec.oxfordjournals.org/content/28/4/335 <br />
<br />
<br />
http://www.bacterio.net/moritella.html <br />
<br />
<br />
Genome structure image:<br />
https://www.patricbrc.org/portal/portal/patric/CircosGenomeViewer?cType=genome&cId=1202962.4 <br />
<br />
<br />
http://jb.asm.org/content/194/22/6296.full.pdf<br />
http://lib.dr.iastate.edu/cgi/viewcontent.cgi?article=1177&context=cbe_pubs<br />
http://www.biochemsoctrans.org/content/28/6/943.long<br />
https://www.researchgate.net/publication/232721452_Genome_Sequence_of_the_Psychrophilic_Deep-Sea_Bacterium_Moritella_marina_MP-1_ATCC_15381<br />
http://aem.asm.org/content/51/4/730.abstract?ijkey=6cfa0071e9fc15e0a007123a571561c86c1354d3&keytype2=tf_ipsecsha<br />
http://link.springer.com/referencework/10.1007%2F978-3-642-30194-0<br />
http://jb.asm.org/content/99/1/326.full.pdf <br />
<br />
<br />
Author</div>Bgalindo6106https://microbewiki.kenyon.edu/index.php?title=Moritella_marina&diff=127479Moritella marina2016-12-02T20:52:08Z<p>Bgalindo6106: </p>
<hr />
<div>Moritella Marina<br />
Higher order taxa<br />
=Classification=<br />
Domain: Bacteria<br />
Phylum: Proteobacteria<br />
Class: Gammaproteobacteria<br />
Order: Alteromonadales<br />
Family: Moritellaceae<br />
Genus: Moritella<br />
<br />
<br />
=Species=<br />
M. japonica, M. yayanosii, M. viscosa, M. profunda, M. abyssi, M. dasanesis<br />
<br />
<br />
=Description and Significance=<br />
Moritella marina is a gram-negative halophilic psychrophilic facultative anaerobe with curved or straight rods, motile polar flagella that produce PUFA’s (polyunsaturated fatty acids) and DHA (docosahexanoic acid). “Twenty-five years ago, a marine bacterium originally designated Vibrio marinus (3) and later renamed Moritella marina MP-1 (14) was reported to produce high levels of DHA (18% of the total fatty acids) (4). Such high levels of DHA presumably provide this bacterium with the ability to maintain appropriate membrane fluidity in the low temperatures and high pressures of its marine environment.”<br />
<br />
<br />
<br />
“Phylogenetic tree of some representatives of the γ-subclass of the class Proteobacteria showing the position of 16S rDNA sequences from some Alteromonas macleodii-related strains and clone sequences from picoplankton DNA from the Mediterranean Sea mesocosm experiment. Sequences of clones 17B161 and 1B161 are available under accession number Y18229 and Y18231, respectively. The tree was calculated by the algorithm of De Soete [16]. The percentage of 500 bootstrap samplings that support branching points above 80% confidence are indicated. The scale bar corresponds to two nucleotide substitutions over 100 sequence positions. The tree was rooted with Roseobacter algicola as an outgroup.” (FEMS, 1998)<br />
=16S Ribosomal RNA Gene Information=<br />
<br />
<br />
<br />
“Sequence variation at positions 73–98 and 181–214 (E. coli nomenclature) within two variable regions of the 16S rDNA of representatives of clone clusters 1–5 and some strains isolated from mesocosm B. Probe target regions are in bold” (FEMS,1998)<br />
=Genome Structure=<br />
<br />
<br />
<br />
Genome structure image link:<br />
https://www.patricbrc.org/portal/portal/patric/CircosGenomeViewer?cType=genome&cId=1202962.4 <br />
Pukall, R., Päuker, O., Buntefuß, D., Ulrichs, G., Lebaron, P., Bernard, L., ... & Stackebrandt, E. (1999). High sequence diversity of Alteromonas macleodii-related cloned and cellular 16S rDNAs from a Mediterranean seawater mesocosm experiment. FEMS Microbiology Ecology, 28(4), 335-344.<br />
http://femsec.oxfordjournals.org/content/28/4/335<br />
<br />
<br />
=Ecology and pathogenesis=<br />
Moritella marina have been found in a variety of cold water marine environments, ranging from the ocean floor to the intestinal tract of marine organisms. Most Moritella species are thought to live mutualistically amongst larger marine organisms, but Moritella viscosa has been noted to cause skin ulcers in some fish (Urakawa, 2014). <br />
=Current Research=<br />
Moritella marina has been noted for having unusually high production of the long chain polyunsaturated fatty acids (PUFAs). When M. marina strain MP-1 was cultured in medium containing cerulenin, a fatty acid synthesis inhibitor, decreases in levels of middle-chain fatty acids and remarkable increases in levels of DHA were observed. These results suggest that the synthesis of middle-chain fatty acids works independently of the synthesis of DHA. M. marina was also found to produce chitonase when induced with chiton (citation). <br />
References<br />
<br />
<br />
And, Kumar B. Kautharapu. "Kumar B. Kautharapu." Genome Sequence of the Psychrophilic Deep-Sea Bacterium Moritella Marina MP-1 (ATCC 15381). N.p., 2012. Web. 02 Dec. 2016.<br />
http://jb.asm.org/content/194/22/6296.full<br />
<br />
<br />
Urakawa, H., Kita-Tsukamoto, K., Steven, S. E., Ohwada, K., & Colwell, R. R. (1998). A proposal to transfer Vibrio marinus (Russell 1891) to a new genus Moritella gen. nov. as Moritella marina comb. nov. FEMS microbiology letters, 165(2), 373-378. http://femsle.oxfordjournals.org/content/165/2/373.full#ref-13<br />
<br />
<br />
Pukall, R., Päuker, O., Buntefuß, D., Ulrichs, G., Lebaron, P., Bernard, L., ... & Stackebrandt, E. (1999). High sequence diversity of Alteromonas macleodii-related cloned and cellular 16S rDNAs from a Mediterranean seawater mesocosm experiment. FEMS Microbiology Ecology, 28(4), 335-344.<br />
http://femsec.oxfordjournals.org/content/28/4/335<br />
<br />
<br />
Stefanidi, E., & Vorgias, C. E. (2008). Molecular analysis of the gene encoding a new chitinase from the marine psychrophilic bacterium Moritella marina and biochemical characterization of the recombinant enzyme. Extremophiles, 12(4), 541-552.<br />
"FEMS Microbiology Ecology." High Sequence Diversity of Alteromonas Macleodii-related Cloned and Cellular 16S RDNAs from a Mediterranean Seawater Mesocosm Experiment | FEMS Microbiology Ecology. N.p., n.d. Web. 02 Dec. 2016.<br />
http://femsec.oxfordjournals.org/content/28/4/335 <br />
<br />
<br />
http://www.bacterio.net/moritella.html <br />
<br />
<br />
Genome structure image:<br />
https://www.patricbrc.org/portal/portal/patric/CircosGenomeViewer?cType=genome&cId=1202962.4 <br />
<br />
<br />
http://jb.asm.org/content/194/22/6296.full.pdf<br />
http://lib.dr.iastate.edu/cgi/viewcontent.cgi?article=1177&context=cbe_pubs<br />
http://www.biochemsoctrans.org/content/28/6/943.long<br />
https://www.researchgate.net/publication/232721452_Genome_Sequence_of_the_Psychrophilic_Deep-Sea_Bacterium_Moritella_marina_MP-1_ATCC_15381<br />
http://aem.asm.org/content/51/4/730.abstract?ijkey=6cfa0071e9fc15e0a007123a571561c86c1354d3&keytype2=tf_ipsecsha<br />
http://link.springer.com/referencework/10.1007%2F978-3-642-30194-0<br />
http://jb.asm.org/content/99/1/326.full.pdf <br />
<br />
<br />
Author</div>Bgalindo6106https://microbewiki.kenyon.edu/index.php?title=Moritella_marina&diff=127478Moritella marina2016-12-02T20:51:44Z<p>Bgalindo6106: </p>
<hr />
<div>Moritella Marina<br />
=Higher order taxa=<br />
=Classification=<br />
Domain: Bacteria<br />
Phylum: Proteobacteria<br />
Class: Gammaproteobacteria<br />
Order: Alteromonadales<br />
Family: Moritellaceae<br />
Genus: Moritella<br />
<br />
<br />
=Species=<br />
M. japonica, M. yayanosii, M. viscosa, M. profunda, M. abyssi, M. dasanesis<br />
<br />
<br />
=Description and Significance=<br />
Moritella marina is a gram-negative halophilic psychrophilic facultative anaerobe with curved or straight rods, motile polar flagella that produce PUFA’s (polyunsaturated fatty acids) and DHA (docosahexanoic acid). “Twenty-five years ago, a marine bacterium originally designated Vibrio marinus (3) and later renamed Moritella marina MP-1 (14) was reported to produce high levels of DHA (18% of the total fatty acids) (4). Such high levels of DHA presumably provide this bacterium with the ability to maintain appropriate membrane fluidity in the low temperatures and high pressures of its marine environment.”<br />
<br />
<br />
<br />
“Phylogenetic tree of some representatives of the γ-subclass of the class Proteobacteria showing the position of 16S rDNA sequences from some Alteromonas macleodii-related strains and clone sequences from picoplankton DNA from the Mediterranean Sea mesocosm experiment. Sequences of clones 17B161 and 1B161 are available under accession number Y18229 and Y18231, respectively. The tree was calculated by the algorithm of De Soete [16]. The percentage of 500 bootstrap samplings that support branching points above 80% confidence are indicated. The scale bar corresponds to two nucleotide substitutions over 100 sequence positions. The tree was rooted with Roseobacter algicola as an outgroup.” (FEMS, 1998)<br />
=16S Ribosomal RNA Gene Information=<br />
<br />
<br />
<br />
“Sequence variation at positions 73–98 and 181–214 (E. coli nomenclature) within two variable regions of the 16S rDNA of representatives of clone clusters 1–5 and some strains isolated from mesocosm B. Probe target regions are in bold” (FEMS,1998)<br />
=Genome Structure=<br />
<br />
<br />
<br />
Genome structure image link:<br />
https://www.patricbrc.org/portal/portal/patric/CircosGenomeViewer?cType=genome&cId=1202962.4 <br />
Pukall, R., Päuker, O., Buntefuß, D., Ulrichs, G., Lebaron, P., Bernard, L., ... & Stackebrandt, E. (1999). High sequence diversity of Alteromonas macleodii-related cloned and cellular 16S rDNAs from a Mediterranean seawater mesocosm experiment. FEMS Microbiology Ecology, 28(4), 335-344.<br />
http://femsec.oxfordjournals.org/content/28/4/335<br />
<br />
<br />
=Ecology and pathogenesis=<br />
Moritella marina have been found in a variety of cold water marine environments, ranging from the ocean floor to the intestinal tract of marine organisms. Most Moritella species are thought to live mutualistically amongst larger marine organisms, but Moritella viscosa has been noted to cause skin ulcers in some fish (Urakawa, 2014). <br />
=Current Research=<br />
Moritella marina has been noted for having unusually high production of the long chain polyunsaturated fatty acids (PUFAs). When M. marina strain MP-1 was cultured in medium containing cerulenin, a fatty acid synthesis inhibitor, decreases in levels of middle-chain fatty acids and remarkable increases in levels of DHA were observed. These results suggest that the synthesis of middle-chain fatty acids works independently of the synthesis of DHA. M. marina was also found to produce chitonase when induced with chiton (citation). <br />
References<br />
<br />
<br />
And, Kumar B. Kautharapu. "Kumar B. Kautharapu." Genome Sequence of the Psychrophilic Deep-Sea Bacterium Moritella Marina MP-1 (ATCC 15381). N.p., 2012. Web. 02 Dec. 2016.<br />
http://jb.asm.org/content/194/22/6296.full<br />
<br />
<br />
Urakawa, H., Kita-Tsukamoto, K., Steven, S. E., Ohwada, K., & Colwell, R. R. (1998). A proposal to transfer Vibrio marinus (Russell 1891) to a new genus Moritella gen. nov. as Moritella marina comb. nov. FEMS microbiology letters, 165(2), 373-378. http://femsle.oxfordjournals.org/content/165/2/373.full#ref-13<br />
<br />
<br />
Pukall, R., Päuker, O., Buntefuß, D., Ulrichs, G., Lebaron, P., Bernard, L., ... & Stackebrandt, E. (1999). High sequence diversity of Alteromonas macleodii-related cloned and cellular 16S rDNAs from a Mediterranean seawater mesocosm experiment. FEMS Microbiology Ecology, 28(4), 335-344.<br />
http://femsec.oxfordjournals.org/content/28/4/335<br />
<br />
<br />
Stefanidi, E., & Vorgias, C. E. (2008). Molecular analysis of the gene encoding a new chitinase from the marine psychrophilic bacterium Moritella marina and biochemical characterization of the recombinant enzyme. Extremophiles, 12(4), 541-552.<br />
"FEMS Microbiology Ecology." High Sequence Diversity of Alteromonas Macleodii-related Cloned and Cellular 16S RDNAs from a Mediterranean Seawater Mesocosm Experiment | FEMS Microbiology Ecology. N.p., n.d. Web. 02 Dec. 2016.<br />
http://femsec.oxfordjournals.org/content/28/4/335 <br />
<br />
<br />
http://www.bacterio.net/moritella.html <br />
<br />
<br />
Genome structure image:<br />
https://www.patricbrc.org/portal/portal/patric/CircosGenomeViewer?cType=genome&cId=1202962.4 <br />
<br />
<br />
http://jb.asm.org/content/194/22/6296.full.pdf<br />
http://lib.dr.iastate.edu/cgi/viewcontent.cgi?article=1177&context=cbe_pubs<br />
http://www.biochemsoctrans.org/content/28/6/943.long<br />
https://www.researchgate.net/publication/232721452_Genome_Sequence_of_the_Psychrophilic_Deep-Sea_Bacterium_Moritella_marina_MP-1_ATCC_15381<br />
http://aem.asm.org/content/51/4/730.abstract?ijkey=6cfa0071e9fc15e0a007123a571561c86c1354d3&keytype2=tf_ipsecsha<br />
http://link.springer.com/referencework/10.1007%2F978-3-642-30194-0<br />
http://jb.asm.org/content/99/1/326.full.pdf <br />
<br />
<br />
Author</div>Bgalindo6106https://microbewiki.kenyon.edu/index.php?title=Moritella_marina&diff=127471Moritella marina2016-12-02T20:44:31Z<p>Bgalindo6106: Created page with "Moritella Marina Higher order taxa =Classification= Domain: Bacteria Phylum: Proteobacteria Class: Gammaproteobacteria Order: Alteromonadales Family: Moritellaceae Genus: Mori..."</p>
<hr />
<div>Moritella Marina<br />
Higher order taxa<br />
=Classification=<br />
Domain: Bacteria<br />
Phylum: Proteobacteria<br />
Class: Gammaproteobacteria<br />
Order: Alteromonadales<br />
Family: Moritellaceae<br />
Genus: Moritella<br />
<br />
<br />
=Species=<br />
M. japonica, M. yayanosii, M. viscosa, M. profunda, M. abyssi, M. dasanesis<br />
<br />
<br />
=Description and Significance=<br />
Moritella marina is a gram-negative halophilic psychrophilic facultative anaerobe with curved or straight rods, motile polar flagella that produce PUFA’s (polyunsaturated fatty acids) and DHA (docosahexanoic acid). “Twenty-five years ago, a marine bacterium originally designated Vibrio marinus (3) and later renamed Moritella marina MP-1 (14) was reported to produce high levels of DHA (18% of the total fatty acids) (4). Such high levels of DHA presumably provide this bacterium with the ability to maintain appropriate membrane fluidity in the low temperatures and high pressures of its marine environment.”<br />
<br />
<br />
<br />
Phylogenetic tree of some representatives of the γ-subclass of the class Proteobacteria showing the position of 16S rDNA sequences from some Alteromonas macleodii-related strains and clone sequences from picoplankton DNA from the Mediterranean Sea mesocosm experiment. Sequences of clones 17B161 and 1B161 are available under accession number Y18229 and Y18231, respectively. The tree was calculated by the algorithm of De Soete [16]. The percentage of 500 bootstrap samplings that support branching points above 80% confidence are indicated. The scale bar corresponds to two nucleotide substitutions over 100 sequence positions. The tree was rooted with Roseobacter algicola as an outgroup.<br />
<br />
=16S Ribosomal RNA Gene Information=<br />
<br />
<br />
<br />
Sequence variation at positions 73–98 and 181–214 (E. coli nomenclature) within two variable regions of the 16S rDNA of representatives of clone clusters 1–5 and some strains isolated from mesocosm B. Probe target regions are in bold<br />
<br />
=Genome Structure=<br />
<br />
<br />
<br />
Genome structure image link:<br />
https://www.patricbrc.org/portal/portal/patric/CircosGenomeViewer?cType=genome&cId=1202962.4 <br />
Pukall, R., Päuker, O., Buntefuß, D., Ulrichs, G., Lebaron, P., Bernard, L., ... & Stackebrandt, E. (1999). High sequence diversity of Alteromonas macleodii-related cloned and cellular 16S rDNAs from a Mediterranean seawater mesocosm experiment. FEMS Microbiology Ecology, 28(4), 335-344.<br />
http://femsec.oxfordjournals.org/content/28/4/335<br />
Cell structure and metabolism<br />
<br />
<br />
=Ecology and pathogenesis=<br />
<br />
Moritella marina have been found in a variety of cold water marine environments, ranging from the ocean floor to the intestinal tract of marine organisms. Most Moritella species are thought to live mutualistically amongst larger marine organisms, but Moritella viscosa has been noted to cause skin ulcers in some fish (Urakawa, 2014). <br />
Current Research<br />
Moritella marina has been noted for having unusually high production of the long chain polyunsaturated fatty acids (PUFAs). When M. marina strain MP-1 was cultured in medium containing cerulenin, a fatty acid synthesis inhibitor, decreases in levels of middle-chain fatty acids and remarkable increases in levels of DHA were observed. These results suggest that the synthesis of middle-chain fatty acids works independently of the synthesis of DHA. M. marina was also found to produce chitonase when induced with chiton (citation). <br />
<br />
=References=<br />
<br />
<br />
And, Kumar B. Kautharapu. "Kumar B. Kautharapu." Genome Sequence of the Psychrophilic Deep-Sea Bacterium Moritella Marina MP-1 (ATCC 15381). N.p., 2012. Web. 02 Dec. 2016.<br />
http://jb.asm.org/content/194/22/6296.full<br />
<br />
<br />
Urakawa, H., Kita-Tsukamoto, K., Steven, S. E., Ohwada, K., & Colwell, R. R. (1998). A proposal to transfer Vibrio marinus (Russell 1891) to a new genus Moritella gen. nov. as Moritella marina comb. nov. FEMS microbiology letters, 165(2), 373-378. http://femsle.oxfordjournals.org/content/165/2/373.full#ref-13<br />
<br />
<br />
Pukall, R., Päuker, O., Buntefuß, D., Ulrichs, G., Lebaron, P., Bernard, L., ... & Stackebrandt, E. (1999). High sequence diversity of Alteromonas macleodii-related cloned and cellular 16S rDNAs from a Mediterranean seawater mesocosm experiment. FEMS Microbiology Ecology, 28(4), 335-344.<br />
http://femsec.oxfordjournals.org/content/28/4/335<br />
<br />
<br />
Stefanidi, E., & Vorgias, C. E. (2008). Molecular analysis of the gene encoding a new chitinase from the marine psychrophilic bacterium Moritella marina and biochemical characterization of the recombinant enzyme. Extremophiles, 12(4), 541-552.<br />
http://www.bacterio.net/moritella.html <br />
<br />
<br />
Genome structure image:<br />
https://www.patricbrc.org/portal/portal/patric/CircosGenomeViewer?cType=genome&cId=1202962.4 <br />
<br />
<br />
http://jb.asm.org/content/194/22/6296.full.pdf<br />
http://lib.dr.iastate.edu/cgi/viewcontent.cgi?article=1177&context=cbe_pubs<br />
http://www.biochemsoctrans.org/content/28/6/943.long<br />
https://www.researchgate.net/publication/232721452_Genome_Sequence_of_the_Psychrophilic_Deep-Sea_Bacterium_Moritella_marina_MP-1_ATCC_15381<br />
http://aem.asm.org/content/51/4/730.abstract?ijkey=6cfa0071e9fc15e0a007123a571561c86c1354d3&keytype2=tf_ipsecsha<br />
http://link.springer.com/referencework/10.1007%2F978-3-642-30194-0<br />
http://jb.asm.org/content/99/1/326.full.pdf <br />
<br />
<br />
Author</div>Bgalindo6106