https://microbewiki.kenyon.edu/api.php?action=feedcontributions&user=Bussand1&feedformat=atommicrobewiki - User contributions [en]2024-03-28T19:04:57ZUser contributionsMediaWiki 1.39.6https://microbewiki.kenyon.edu/index.php?title=Alcanivorax&diff=30143Alcanivorax2008-04-21T16:17:01Z<p>Bussand1: /* Ecology */</p>
<hr />
<div>[[Image:AlcVorax PROCARYOTES01b-1.JPG|thumbnail|300px|Figure 1. ''Alcanivorax borkumensis''. Image from Helmholtz Centre for Infection Research[http://www.helmholtz-hzi.de/en/news_public_relation/press_releases/view/article/complete/oil_tanker_accidents_as_a_source_of_food/]]]<br />
<br />
==Classification==<br />
<br />
'''Bacteria'''; Phylum: '''Proteobacteria'''; Class: '''Gammaproteobacteria'''; Order: '''Oceanospirillales'''; Family: '''Alcanivoracaceae'''<br />
<br />
===Species===<br />
<br />
{|<br />
| height="10" bgcolor="#FFDF95" |<br />
'''NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Tree&id=2&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy]'''<br />
|}<br />
<br />
*''Alcanivorax balearicum''<br />
*''Alcanivorax borkumensis''<br />
*''Alcanivorax dieselolei''<br />
*''Alcanivorax indicus''<br />
*''Alcanivorax jadensis''<br />
*''Alcanivorax venustensis''<br />
<br />
==Description and Significance==<br />
[[Image:PWS tanker oil spill.jpg|thumbnail|200px|Figure 2. Supertanker Exxon Valdez grounded on Bligh Reef which released 11 million gallons of crude oil into the water. This oil-contaminated seawater is the preferred habitat for ''Alcanivorax''. Image from USGS[http://menlocampus.wr.usgs.gov/50years/accomplishments/oil.html]]]<br />
<br />
''Alcanivorax'', first described in 1998, is a Gram-negative, halophilic, aerobic, rod-shaped, oil-degrading marine bacterium that is found in low abundances in unpolluted environments in the upper layers of the ocean, but quickly becomes the predominant microbe in oil-contaminated open oceans and coastal waters when nitrogen and phosphorus are not limiting [2]. When conditions in these moderately halophilic environments are right, ''Alcanivorax'' may make up 80-90% of the oil-degrading microbes present in the area [4]. It is described as a non-motile bactertium which is true for species such as ''Alcanivorax borkumensis'', but other species such as ''Alcanivorax venustensis'' were described to be motile by polar flagella [1]. The optimial conditions described for A.borkumensis growth include temperatures in the range of 20-30 degrees celsius, and a NaCl concentration of 3-10%.<br />
<br />
As a result of their profound ability to degrade and live predominately on alkanes, as well as to become the dominant microbes in oil-contaminated areas, ''Alcanivorax'' plays a huge role in the biological cleanup of oil-contaminated environments. These oil-contaminated environments in the ocean are largely due to anthropogenic sources such as oil spills caused by tankers accidents (Figure 2), and cause serious ecological damage to plants and animals on the coast as well as other inhabitants of the ocean. Microbes such as ''Alcanivorax'' provide a major route for the breakdown of these pollutants, and demonstrate how marine bacteria keep the environment in check. Of all the ''Alcanivorax'' species and other oil-degrading microbes, ''Alcanivorax borkumensis'' is one of the most important worldwide due to the fact it produces a wide variety of very efficient oil-degrading enzymes. With this knowledge, ''A. borkumensis'' could provide a useful tool for bioremediation of oil spills.<br />
<br />
==Genome Structure==<br />
[[Image:Lorenzo.gif|thumbnail|200px|Figure 3. Mechanisms for oil degradation and survival encoded by the ''A. borkumensis SK2'' genome. Image from Victor de Lorenzo[http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html]]]<br />
<br />
The ''Alcanivorax borkumensis'' strain SK2, isolated from a seawater sediment sample in the North Sea at a site located near the Isle of Borkum, was the first hydrocarbonoclastic bacterium to be sequenced and was completed by Susanne Schneiker et al. It's genome consists of a single circular chromosome with 3,120,143 base pairs and an average G+C content of 54.7%. The genomic analysis of ''A. borkumensis SK2'' revealed several new insights into the bacterium's role for (i) n-alkane degradation (which includes metabolism, biosurfactant production and biofilm production), (ii) it's system for capturing or scavenging the small amounts of nitrogen, phosphorous, sulfur, and other elements in a nutrient-poor marine environment which allows for more efficient alkane degradation due to their main limitation of nutrient availability, (iii) as well as means for coping with stress factors such as high salt contents and high UV radiation since it thrives mostly in the upper layers in the ocean where UV light is encountered (Figure 3).<br />
<br />
It's genome encodes several systems for the catabolism of hydrocarbons which allow the bacertium to degrade all sorts of alkanes such as AlkB1 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C5-C12, and AlkB2 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C8 to C16. Both these systems are located close to the origin of replication of the chromosome. ''A. borkumensis'' is also able to degrade alkanes up to C32, branched aliphatic hydrocarbons, isoprenoid hydrocarbons such as phytane, as well as alkylarenes and alkylcycloalkanes. Thus, the genome encodes for a broad spectrum of systems for the catabolism of hydrocarbons, giving it a competitive advantage over other oil-degrading marine microbial communities. To deal with the damaging effects of UV light, ''A. borkumensis'' has a number of genes that reduce the damage. These include the full genes for DNA alkylation, recombinational and nucleotide excision repair, base excision repair, as well as the SOS response [4].<br />
<br />
==Cell Structure, Metabolism and Life Cycle==<br />
''Alcanivorax borkumensis'', a Gram-negative, rod-shaped chemoorganotroph, is able to use n-alkanes as its principle carbon and energy source by use of the broad spectrum of oil-degrading enzymes it possesses, but they can also use a limited number of organic compounds such as aliphatic hydrocarbons, volatile fatty acids, and pyruvate. However, it cannot utilize carbon sources such as sugars or amino acids. Cells grown with pyruvate were observed to be 2.0-3.0 micrometers in length and 0.4-07 micrometers in diameter, however, cells were shorter (1.0-1.5 micrometers in length) when cells were grown with n-alkanes as the carbon source (see Figure 1) [5]. When the slow growing ''A. borkumensis'' uses n-alkanes exclusively, the microbes produce extracellular and membrane-bound surface-active glucose lipids called biosurfactants. These biosurfactants reduce the surface tension of water from 72 to 29 mN m-1 and act as natural emulsifiers which enhances the break up of oil-in-water emulsions [4,5]. Due to the low solubility of oil in water, most oil degradation takes place at the oil-water interface where ''A. borkumensis'' attaches and forms a biofilm around the oil droplets as depicted in Figure 3.<br />
<br />
==Ecology==<br />
[[Image:OilContamination.jpg|thumbnail|200px|Figure 4. Oil spills in the ocean affect more than the aquatic environment. ''Alcanivorax'' helps reduce the damage on ecosystem health.]]<br />
<br />
''Alcanivorix'' is a novel species living in the oceans that plays a major role in keeping our pristine oceans as well as the inhabitants of the ocean and the inhabitants of the coastal regions in good health. It has been detected worldwide in places such as the Mediterranean Sea, Pacific Ocean, and the Arctic Sea [4]. In seawater with high concentrations of n-alkanes (as a result of oil spills, natural oil fields, and/or processing plants), ''Alcanivorax'' quickly becomes the predominant microbial community and is found in higher populations when compared to ''Alcanivorax'' in unpolluted seawater. There have been several recent fields studies on bacterial community dynamics and hydrocarbon degradation in coastal areas contaminated with oil. These field studies have demonstrated the immense importance of ''Alcanivorax'' (particularly ''A. Borkumensis'') in oil-spill bioremediation [2].<br />
<br />
==References==<br />
[1] [http://ijs.sgmjournals.org/cgi/content/abstract/53/1/331 Fernandez-Martinez, Javier, Maria J. Pujalte, Jesus Garcia-Martinez, Manuel Mata, Esperanza Garay, and Francisco Rodriguez-Valera. "Description of ''Alcanivorax Venustensis'' sp. nov. and Reclassification of ''Fundibacter Jadensis'' DSM 12178T (Bruns and Berthe-Corti 1999) As ''Alcanivorax Jadensis'' comb. nov., Members of the Emended Genus ''Alcanivorax''." International Journal of Systematic and Evolutionary Microbiology 53 (2003): 331-338.]<br />
<br />
[2] [http://www.blackwell-synergy.com/doi/pdf/10.1046/j.1468-2920.2003.00468.x Hara, Akihiro, Kazuaki Syutsubo, and Shigeaki Harayama. "''Alcanivorax'' Which Prevails In Oil-contaminated Seawater Exhibits Broad Substrate Specificity For Alkane Degradation." Environmental Microbiology 5.9 (2003): 746-753.]<br />
<br />
[3] [http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html Lorenzo, Víctor De. "Blueprint of an Oil-eating Bacterium." Nature Biotechnology 24 (2006): 952-953.]<br />
<br />
[4] [http://www.ncbi.nlm.nih.gov/pubmed/16878126 Schneiker, S. et al. "Genome Sequence of the Ubiquitous Hydrocarbon-degrading Marine Bacterium A''lcanivorax Borkumensis''." Nature Biotechnology 24 (2006): 997-1004.]<br />
<br />
[5] [http://ijs.sgmjournals.org/cgi/content/abstract/48/2/339 Yakimov, Michail M., Peter N. Golyshin, Siegmund Lang, Edward R. B. Moore, Wolf-Rainer Abraham, Heinrich Lunsdorf, and Kenneth N. Timmis. "''Alcanivorax Borkumensis'' gen. nov., sp. nov., A New, Hydrocarbon-degrading And Surfactant-producing Marine Bacterium." International Journal of Systematic Bacteriology 48 (1998): 339-348.]<br />
<br />
==Author==<br />
Page authored by Andrew Buss, student of [http://www.kbs.msu.edu/faculty/lennon/ Prof. Jay Lennon] at Michigan State University.</div>Bussand1https://microbewiki.kenyon.edu/index.php?title=Alcanivorax&diff=29854Alcanivorax2008-04-18T03:00:30Z<p>Bussand1: /* Cell Structure, Metabolism and Life Cycle */</p>
<hr />
<div>[[Image:AlcVorax PROCARYOTES01b-1.JPG|thumbnail|300px|Figure 1. ''Alcanivorax borkumensis''. Image from Helmholtz Centre for Infection Research[http://www.helmholtz-hzi.de/en/news_public_relation/press_releases/view/article/complete/oil_tanker_accidents_as_a_source_of_food/]]]<br />
<br />
==Classification==<br />
<br />
'''Bacteria'''; Phylum: '''Proteobacteria'''; Class: '''Gammaproteobacteria'''; Order: '''Oceanospirillales'''; Family: '''Alcanivoracaceae'''<br />
<br />
===Species===<br />
<br />
{|<br />
| height="10" bgcolor="#FFDF95" |<br />
'''NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Tree&id=2&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy]'''<br />
|}<br />
<br />
*''Alcanivorax balearicum''<br />
*''Alcanivorax borkumensis''<br />
*''Alcanivorax dieselolei''<br />
*''Alcanivorax indicus''<br />
*''Alcanivorax jadensis''<br />
*''Alcanivorax venustensis''<br />
<br />
==Description and Significance==<br />
[[Image:PWS tanker oil spill.jpg|thumbnail|200px|Figure 2. Supertanker Exxon Valdez grounded on Bligh Reef which released 11 million gallons of crude oil into the water. This oil-contaminated seawater is the preferred habitat for ''Alcanivorax''. Image from USGS[http://menlocampus.wr.usgs.gov/50years/accomplishments/oil.html]]]<br />
<br />
''Alcanivorax'', first described in 1998, is a Gram-negative, halophilic, aerobic, rod-shaped, oil-degrading marine bacterium that is found in low abundances in unpolluted environments in the upper layers of the ocean, but quickly becomes the predominant microbe in oil-contaminated open oceans and coastal waters when nitrogen and phosphorus are not limiting [2]. When conditions in these moderately halophilic environments are right, ''Alcanivorax'' may make up 80-90% of the oil-degrading microbes present in the area [4]. It is described as a non-motile bactertium which is true for species such as ''Alcanivorax borkumensis'', but other species such as ''Alcanivorax venustensis'' were described to be motile by polar flagella [1]. The optimial conditions described for A.borkumensis growth include temperatures in the range of 20-30 degrees celsius, and a NaCl concentration of 3-10%.<br />
<br />
As a result of their profound ability to degrade and live predominately on alkanes, as well as to become the dominant microbes in oil-contaminated areas, ''Alcanivorax'' plays a huge role in the biological cleanup of oil-contaminated environments. These oil-contaminated environments in the ocean are largely due to anthropogenic sources such as oil spills caused by tankers accidents (Figure 2), and cause serious ecological damage to plants and animals on the coast as well as other inhabitants of the ocean. Microbes such as ''Alcanivorax'' provide a major route for the breakdown of these pollutants, and demonstrate how marine bacteria keep the environment in check. Of all the ''Alcanivorax'' species and other oil-degrading microbes, ''Alcanivorax borkumensis'' is one of the most important worldwide due to the fact it produces a wide variety of very efficient oil-degrading enzymes. With this knowledge, ''A. borkumensis'' could provide a useful tool for bioremediation of oil spills.<br />
<br />
==Genome Structure==<br />
[[Image:Lorenzo.gif|thumbnail|200px|Figure 3. Mechanisms for oil degradation and survival encoded by the ''A. borkumensis SK2'' genome. Image from Victor de Lorenzo[http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html]]]<br />
<br />
The ''Alcanivorax borkumensis'' strain SK2, isolated from a seawater sediment sample in the North Sea at a site located near the Isle of Borkum, was the first hydrocarbonoclastic bacterium to be sequenced and was completed by Susanne Schneiker et al. It's genome consists of a single circular chromosome with 3,120,143 base pairs and an average G+C content of 54.7%. The genomic analysis of ''A. borkumensis SK2'' revealed several new insights into the bacterium's role for (i) n-alkane degradation (which includes metabolism, biosurfactant production and biofilm production), (ii) it's system for capturing or scavenging the small amounts of nitrogen, phosphorous, sulfur, and other elements in a nutrient-poor marine environment which allows for more efficient alkane degradation due to their main limitation of nutrient availability, (iii) as well as means for coping with stress factors such as high salt contents and high UV radiation since it thrives mostly in the upper layers in the ocean where UV light is encountered (Figure 3).<br />
<br />
It's genome encodes several systems for the catabolism of hydrocarbons which allow the bacertium to degrade all sorts of alkanes such as AlkB1 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C5-C12, and AlkB2 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C8 to C16. Both these systems are located close to the origin of replication of the chromosome. ''A. borkumensis'' is also able to degrade alkanes up to C32, branched aliphatic hydrocarbons, isoprenoid hydrocarbons such as phytane, as well as alkylarenes and alkylcycloalkanes. Thus, the genome encodes for a broad spectrum of systems for the catabolism of hydrocarbons, giving it a competitive advantage over other oil-degrading marine microbial communities. To deal with the damaging effects of UV light, ''A. borkumensis'' has a number of genes that reduce the damage. These include the full genes for DNA alkylation, recombinational and nucleotide excision repair, base excision repair, as well as the SOS response [4].<br />
<br />
==Cell Structure, Metabolism and Life Cycle==<br />
''Alcanivorax borkumensis'', a Gram-negative, rod-shaped chemoorganotroph, is able to use n-alkanes as its principle carbon and energy source by use of the broad spectrum of oil-degrading enzymes it possesses, but they can also use a limited number of organic compounds such as aliphatic hydrocarbons, volatile fatty acids, and pyruvate. However, it cannot utilize carbon sources such as sugars or amino acids. Cells grown with pyruvate were observed to be 2.0-3.0 micrometers in length and 0.4-07 micrometers in diameter, however, cells were shorter (1.0-1.5 micrometers in length) when cells were grown with n-alkanes as the carbon source (see Figure 1) [5]. When the slow growing ''A. borkumensis'' uses n-alkanes exclusively, the microbes produce extracellular and membrane-bound surface-active glucose lipids called biosurfactants. These biosurfactants reduce the surface tension of water from 72 to 29 mN m-1 and act as natural emulsifiers which enhances the break up of oil-in-water emulsions [4,5]. Due to the low solubility of oil in water, most oil degradation takes place at the oil-water interface where ''A. borkumensis'' attaches and forms a biofilm around the oil droplets as depicted in Figure 3.<br />
<br />
==Ecology==<br />
[[Image:OilContamination.jpg|thumbnail|200px|Figure 4. Oil spills in the ocean affect more than the aquatic environment. ''Alcanivorax'' helps reduce the damage on ecosystem health.]]<br />
<br />
''Alcanivorix'' is a novel species living in the oceans that plays a major role in keeping our pristine oceans as well as the inhabitants of the ocean and the inhabitants of the coastal regions in good health. It has been detected worldwide in places such as the Mediterranean Sea, Pacific Ocean, and the Arctic Sea [4]. In seawater with high concentrations of n-alkanes (as a result of oil spills, natural oil fields, and/or processing plants), ''Alcanivorax'' quickly becomes the predominant microbial community and is found in higher populations when compared to ''Alcanivorax'' in unpolluted seawater. There have been several recent fields studies on bacterial on community dynamics and hydrocarbon degradation in coastal areas contaminated with oil. These field studies have demonstrated the immense importance of ''Alcanivorax'' (particularly ''A. Borkumensis'') in oil-spill bioremediation [2].<br />
<br />
==References==<br />
[1] [http://ijs.sgmjournals.org/cgi/content/abstract/53/1/331 Fernandez-Martinez, Javier, Maria J. Pujalte, Jesus Garcia-Martinez, Manuel Mata, Esperanza Garay, and Francisco Rodriguez-Valera. "Description of ''Alcanivorax Venustensis'' sp. nov. and Reclassification of ''Fundibacter Jadensis'' DSM 12178T (Bruns and Berthe-Corti 1999) As ''Alcanivorax Jadensis'' comb. nov., Members of the Emended Genus ''Alcanivorax''." International Journal of Systematic and Evolutionary Microbiology 53 (2003): 331-338.]<br />
<br />
[2] [http://www.blackwell-synergy.com/doi/pdf/10.1046/j.1468-2920.2003.00468.x Hara, Akihiro, Kazuaki Syutsubo, and Shigeaki Harayama. "''Alcanivorax'' Which Prevails In Oil-contaminated Seawater Exhibits Broad Substrate Specificity For Alkane Degradation." Environmental Microbiology 5.9 (2003): 746-753.]<br />
<br />
[3] [http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html Lorenzo, Víctor De. "Blueprint of an Oil-eating Bacterium." Nature Biotechnology 24 (2006): 952-953.]<br />
<br />
[4] [http://www.ncbi.nlm.nih.gov/pubmed/16878126 Schneiker, S. et al. "Genome Sequence of the Ubiquitous Hydrocarbon-degrading Marine Bacterium A''lcanivorax Borkumensis''." Nature Biotechnology 24 (2006): 997-1004.]<br />
<br />
[5] [http://ijs.sgmjournals.org/cgi/content/abstract/48/2/339 Yakimov, Michail M., Peter N. Golyshin, Siegmund Lang, Edward R. B. Moore, Wolf-Rainer Abraham, Heinrich Lunsdorf, and Kenneth N. Timmis. "''Alcanivorax Borkumensis'' gen. nov., sp. nov., A New, Hydrocarbon-degrading And Surfactant-producing Marine Bacterium." International Journal of Systematic Bacteriology 48 (1998): 339-348.]<br />
<br />
==Author==<br />
Page authored by Andrew Buss, student of [http://www.kbs.msu.edu/faculty/lennon/ Prof. Jay Lennon] at Michigan State University.</div>Bussand1https://microbewiki.kenyon.edu/index.php?title=Taxonomy_Index&diff=29801Taxonomy Index2008-04-14T21:40:51Z<p>Bussand1: </p>
<hr />
<div>Includes a selection of current taxa from '''[[Microbial Biorealm]]''' and '''[[Viral Biorealm]]'''. Note: Many new pages authored by students have not yet been indexed here. To check whether a taxon has been covered, type the genus name at left and click "Search." The "Go" button only calls up pages whose title matches exactly what you typed, whereas "Search" calls up any page that mentions your item.<br><br />
<center><table border="0" cellpadding="2" cellspacing="2" width="100%"><br />
<caption> </caption><br />
<br />
<tr><br />
<td align="center" bgcolor="#ffccff" height="1626" valign="top" width="25%"> <b><font size="+2">Bacteria</font></b><br />
''[[Acetobacter]]''<br><br />
''[[Acinetobacter]]''<br><br />
''[[Aeromonas]]''<br><br />
''[[Agrobacterium]]''<br><br />
''[[Alcaligenes]]''<br><br />
''[[Alcanivorax]]''<br><br />
''[[Anabaena]]''<br><br />
''[[Aquifex]]''<br><br />
''[[Arthrobacter]]''<br><br />
''[[Azoarcus]]''<br><br />
''[[Azotobacter]]''<br><br />
''[[Bacillus]]''<br><br />
''[[Bacteroides]]''<br><br />
''[[Bdellovibrio]]''<br><br />
''[[Beggiatoa]]''<br><br />
''[[Bifidobacterium]]''<br><br />
''[[Borrelia]]''<br><br />
''[[Bradyrhizobium]]''<br><br />
''[[Brucella]]''<br><br />
''[[Burkholderia]]''<br><br />
''[[Campylobacter]]''<br><br />
''[[Candidatus Chloracidobacterium]]''<br><br />
''[[Caulobacter]]''<br><br />
''[[Chlamydia]]''<br><br />
''[[Chlorobium]]''<br><br />
''[[Chloroflexus]]''<br><br />
''[[Chroococcus]]''<br><br />
''[[Clostridium]]''<br><br />
''[[Corynebacterium]]''<br><br />
''[[Coxiella]]''<br><br />
''[[Dehalobacter]]''<br><br />
''[[Deinococcus]]''<br><br />
''[[Desulfosarcina]]''<br><br />
''[[Desulfovibrio]]''<br><br />
''[[Desulfuromonas]]''<br><br />
''[[Enterobacter]]''<br><br />
''[[Enterococcus]]''<br><br />
''[[Epulopiscium]]''<br><br />
''[[Erysipelothrix]]''<br />
''[[Erythrobacter]]''<br><br />
''[[Erythromicrobium]]''<br><br />
''[[Escherichia]]''<br><br />
''[[Flavobacterium]]''<br><br />
''[[Flexibacter]]''<br><br />
''[[Frankia]]''<br><br />
''[[Fusobacterium]]''<br><br />
''[[Gallionella]]''<br><br />
''[[Gemmata]]''<br><br />
''[[Geobacter]]''<br><br />
''[[Halomonas]]''<br><br />
''[[Helicobacter]]''<br><br />
''[[Klebsiella]]''<br><br />
''[[Lactobacillus]]''<br><br />
''[[Legionella]]''<br><br />
''[[Leptospira]]''<br><br />
''[[Leptospirillum]]''<br><br />
''[[Leptothrix]]''<br><br />
''[[Listeria]]''<br><br />
''[[Marinobacter]]''<br><br />
''[[Magnetotactic]]''<br><br />
''[[Merismopedia]]''<br><br />
''[[Methylobacterium]]''<br><br />
''[[Micrococcus]]''<br><br />
''[[Mitochondria]]''<br><br />
''[[Mycobacterium]]''<br><br />
''[[Mycoplasma]]''<br><br />
''[[Myxococcus]]''<br><br />
''[[Neisseria]]''<br><br />
''[[Nitrosococcus]]''<br><br />
''[[Nitrosomonas]]''<br><br />
''[[Nitrospira]]''<br><br />
''[[Nostoc]]''<br><br />
''[[Pasteurella]]''<br><br />
''[[Petrotoga]]''<br><br />
''[[Pirellula]]''<br><br />
''[[Planctomyces]]''<br><br />
''[[Pleurocapsa]]''<br><br />
''[[Porphyromonas]]''<br><br />
''[[Prevotella]]''<br><br />
''[[Prochlorococcus]]''<br><br />
''[[Prochloron]]''<br><br />
''[[Propionibacterium]]''<br><br />
''[[Prosthecobacter]]''<br><br />
''[[Prosthecomicrobium]]''<br><br />
''[[Proteobacteria]]''<br><br />
''[[Pseudomonas]]''<br><br />
''[[Psychrobacter]]''<br><br />
''[[Rhizobium]]''<br><br />
''[[Rhodobacter]]''<br><br />
''[[Rhodopseudomonas]]''<br><br />
''[[Rhodospirillum]]''<br><br />
''[[Rickettsia]]''<br><br />
''[[Roseobacter]]''<br><br />
''[[Ruminobacter]]''<br><br />
''[[Ruminococcus]]''<br><br />
''[[Salmonella]]''<br><br />
''[[Shigella]]''<br><br />
''[[Sinorhizobium]]''<br><br />
''[[Sphaerotilus]]''<br><br />
''[[Sphingomonas]]''<br><br />
''[[Spirillum]]''<br><br />
''[[Spirulina]]''<br><br />
''[[Staphylococcus]]''<br><br />
''[[Streptococcus]]''<br><br />
''[[Streptomyces]]''<br><br />
''[[Succinomonas]]''<br><br />
''[[Succinivibrio]]''<br><br />
''[[Synechococcus]]''<br><br />
''[[Thermodesulfobacterium]]''<br><br />
''[[Thermotoga]]''<br><br />
''[[Thermus]]''<br><br />
''[[Thiobacillus]]''<br><br />
''[[Thiocapsa]]''<br><br />
''[[Thiomargarita]]''<br><br />
''[[Treponema]]''<br><br />
''[[Trichodesmium]]''<br><br />
''[[Verrucomicrobium]]''<br><br />
''[[Vibrio]]''<br><br />
''[[Wigglesworthia]]''<br><br />
''[[Wolbachia]]''<br><br />
''[[Xanthomonas]]''<br><br />
''[[Yersinia]]''<br><br />
<br />
<br />
<br />
<td align="center" cellpadding="2" cellspacing="2" bgcolor="#ccccff" valign="top" width="25%"> <font size="+2">'''Archaea'''</font><br />
''[[Acidilobus]]''<br><br />
''[[Aeropyrum]]''<br><br />
''[[Archaeoglobus]]''<br><br />
''[[Caldisphaera]]''<br><br />
''[[Desulfurococcus]]''<br><br />
''[[Ferroplasma]]''<br><br />
''[[Haloarcula]]''<br><br />
''[[Halobacterium]]''<br><br />
''[[Halococcus]]''<br><br />
''[[Haloferax]]''<br><br />
''[[Haloquadra]]''<br><br />
''[[Halorhabdus]]''<br><br />
''[[Hyperthermus]]''<br><br />
''[[Ignicoccus]]''<br><br />
''[[Methanococcus]]''<br><br />
''[[Methanoculleus]]''<br><br />
''[[Methanofollis]]''<br><br />
''[[Methanogens]]''<br><br />
''[[Methanopyrus]]''<br><br />
''[[Methanosarcina]]''<br><br />
''[[Methanosphaera]]'' <br /><br />
''[[Methanothermobacter]]''<br><br />
''[[Nanoarchaeum]]''<br><br />
''[[Natronococcus]]''<br><br />
''[[Natronomonas]]'' <br><br />
''[[Picrophilus]]'' <br><br />
''[[Pyrobaculum]]'' <br><br />
''[[Pyrococcus]]''<br><br />
''[[Pyrodictium]]''<br><br />
''[[Sulfolobus]]''<br><br />
''[[Sulfurisphaera]]''<br><br />
''[[Thermococcus]]'' <br><br />
''[[Thermoplasma]]''<br><br />
''[[Thermoproteus]]''<br><br />
''[[Thermosphaera]]''<br><br />
''[[Vulcanisaeta]]'' <br><br />
</td><br />
<br />
<td align="center" cellspacing="2" cellpadding="2" bgcolor="#ffffcc" valign="top" width="25%"><font size="+2">'''Eukarya'''</font><br />
''[[Acrasids]]''<br><br />
''[[Actinophrydae]]''<br><br />
''[[Alexandrium]]''<br><br />
''[[Ameba]]''<br><br />
''[[Apicomplex]]''<br><br />
''[[Ascomycota]]''<br><br />
''[[Aspergillus]]''<br><br />
''[[Babesia]]''<br><br />
''[[Basidiomycota]]''<br><br />
''[[Bacillariophyta]]''<br><br />
''[[Bicosoecids]]''<br><br />
''[[Ceratium]]''<br><br />
''[[Charophyta]]''<br><br />
''[[Choanoflagellata]]''<br><br />
''[[Chrysophyceae]]''<br><br />
''[[Chrysophyta]]''<br><br />
''[[Chlorophyta]]''<br><br />
''[[Chytridiomycota]]''<br><br />
''[[Chrysophyta]]''<br><br />
''[[Ciliophora]]''<br><br />
''[[Cryptococcus]]''<br><br />
''[[Cryptosporidium]]''<br><br />
''[[Cyclospora]]''<br><br />
''[[Desulfurococcus]]''<br><br />
''[[Dictiostelida|Dictyostelida]]''<br><br />
''[[Didinium]]''<br><br />
''[[Dinoflagellata]]''<br><br />
''[[Diplomonada]]''<br><br />
''[[Emiliana|Emiliania]]''<br><br />
''[[Euglenozoa]]''<br><br />
''[[Euglyphids]]''<br><br />
''[[Foraminifera]]''<br><br />
''[[Giardia]]''<br><br />
''[[Labyrinthula]]''<br><br />
''[[Leishmania]]''<br><br />
''[[Leukocytozoon]]''<br><br />
''[[Magnaporthe]]''<br><br />
''[[Metazoa]]''<br><br />
''[[Microsporidia]]''<br><br />
''[[Mixotricha]]''<br><br />
''[[Morchella]]''<br><br />
''[[Myxogastria]]''<br><br />
''[[Nano- and Picoeukaryotes|Nanoeukaryotes]]''<br><br />
''[[Neurospora]]''<br><br />
''[[Oomycota]]''<br><br />
''[[Opalinids]]''<br><br />
''[[Oxymonads]]''<br><br />
''[[Parabasalia]]''<br><br />
''[[Paramecium]]''<br><br />
''[[Pelobionta]]''<br><br />
''[[Penecillium|Penicillium]]''<br><br />
''[[Pfiesteria]]''<br><br />
''[[Phaeophyceae]]''<br><br />
''[[Nano- and Picoeukaryotes|Picoeukaryotes]]''<br><br />
''[[Plasmodium]]''<br><br />
''[[Protostelida]]''<br><br />
''[[Radiolaria]]''<br><br />
''[[Retortamonads]]''<br><br />
''[[Rhodophyta]]''<br><br />
''[[Saccharomyces]]''<br><br />
''[[Sarcocystis]]''<br><br />
''[[Stentor]]''<br><br />
''[[Suctoria]]''<br><br />
''[[Parabasalia|Trichomonas]]''<br><br />
''[[Trypanosoma]]''<br><br />
''[[Ustilago]]''<br><br />
''[[Vorticella]]''<br><br />
''[[Xanthophyceae]]''<br><br />
''[[Zooxanthellae]]''<br><br />
''[[Zygomycota]]''<br><br />
<br />
<td align="center"<!-- Suggest using a non yellow color yellow is already used. Maybe a light green #caffbc like that would work. Drew T. 6.2.06 --> bgcolor="#ffff99" height="1626" valign="top" width="25%"> <b><font size="+2">Viruses</font></b><br />
[[Adenoviridae]]<br><br />
[[Ampullaviridae]]<br><br />
''[[Aphthovirus]]''<br><br />
[[Arenaviridae]]<br><br />
[[Baculoviridae]]<br><br />
''[[Badnavirus]]''<br><br />
[[Bicaudaviridae]]<br><br />
[[Birnaviridae]]<br><br />
[[Bromoviridae]]<br><br />
''[[Bromovirus]]''<br><br />
[[Bunyaviridae]]<br><br />
[[Caulimoviridae]]<br><br />
''[[Caulimovirus]]''<br><br />
''[[Circovirus]]''<br><br />
''[[Cladosporium]]''<br />
''[[Coronavirus]]''<br><br />
[[Cystoviridae]]<br><br />
''[[Enterovirus]]''<br><br />
[[Filoviridae]]<br><br />
[[Flaviviridae]]<br><br />
[[Fuselloviridae]]<br><br />
[[Geminiviridae]]<br><br />
[[Globuloviridae]]<br><br />
[[Guttaviridae]]<br><br />
''[[Hantavirus]]''<br><br />
''[[Hepacivirus]]''<br><br />
[[Hepadnaviridae]]<br><br />
''[[Hepatovirus]]''<br><br />
''[[Hepatitis B virus]]''<br><br />
[[Herpesviridae]]<br><br />
''[[Human immunodeficiency virus]]''<br><br />
[[Inoviridae]]<br><br />
[[Iridoviridae]]<br><br />
[[Leviviridae]]<br><br />
[[Lipothrixviridae]]<br><br />
[[Myoviridae]]<br><br />
[[Orthomyxoviridae]]<br><br />
''[[Orthoreovirus]]''<br><br />
[[Papillomaviridae]]<br><br />
[[Paramyxoviridae]]<br><br />
[[Parvoviridae]]<br><br />
[[Phycodnaviridae]]<br><br />
[[Picornaviridae]]<br><br />
[[Potyviridae]]<br><br />
''[[Potyvirus]]''<br><br />
[[Poxviridae]]<br><br />
[[Reoviridae]]<br><br />
[[Retroviridae]]<br><br />
[[Rhabdoviridae]]<br><br />
''[[Rhinovirus]]''<br><br />
''[[Rotavirus]]''<br><br />
[[Rudiviridae]]<br><br />
[[Siphoviridae]]<br><br />
[[Tectiviridae]]<br><br />
''[[Tobamovirus]]''<br><br />
[[Togaviridae]]<br><br />
''[[Varicosavirus]]''<br><br />
</td></div>Bussand1https://microbewiki.kenyon.edu/index.php?title=Alcanivorax&diff=29800Alcanivorax2008-04-12T16:37:52Z<p>Bussand1: /* Ecology */</p>
<hr />
<div>[[Image:AlcVorax PROCARYOTES01b-1.JPG|thumbnail|300px|Figure 1. ''Alcanivorax borkumensis''. Image from Helmholtz Centre for Infection Research[http://www.helmholtz-hzi.de/en/news_public_relation/press_releases/view/article/complete/oil_tanker_accidents_as_a_source_of_food/]]]<br />
<br />
==Classification==<br />
<br />
'''Bacteria'''; Phylum: '''Proteobacteria'''; Class: '''Gammaproteobacteria'''; Order: '''Oceanospirillales'''; Family: '''Alcanivoracaceae'''<br />
<br />
===Species===<br />
<br />
{|<br />
| height="10" bgcolor="#FFDF95" |<br />
'''NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Tree&id=2&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy]'''<br />
|}<br />
<br />
*''Alcanivorax balearicum''<br />
*''Alcanivorax borkumensis''<br />
*''Alcanivorax dieselolei''<br />
*''Alcanivorax indicus''<br />
*''Alcanivorax jadensis''<br />
*''Alcanivorax venustensis''<br />
<br />
==Description and Significance==<br />
[[Image:PWS tanker oil spill.jpg|thumbnail|200px|Figure 2. Supertanker Exxon Valdez grounded on Bligh Reef which released 11 million gallons of crude oil into the water. This oil-contaminated seawater is the preferred habitat for ''Alcanivorax''. Image from USGS[http://menlocampus.wr.usgs.gov/50years/accomplishments/oil.html]]]<br />
<br />
''Alcanivorax'', first described in 1998, is a Gram-negative, halophilic, aerobic, rod-shaped, oil-degrading marine bacterium that is found in low abundances in unpolluted environments in the upper layers of the ocean, but quickly becomes the predominant microbe in oil-contaminated open oceans and coastal waters when nitrogen and phosphorus are not limiting [2]. When conditions in these moderately halophilic environments are right, ''Alcanivorax'' may make up 80-90% of the oil-degrading microbes present in the area [4]. It is described as a non-motile bactertium which is true for species such as ''Alcanivorax borkumensis'', but other species such as ''Alcanivorax venustensis'' were described to be motile by polar flagella [1]. The optimial conditions described for A.borkumensis growth include temperatures in the range of 20-30 degrees celsius, and a NaCl concentration of 3-10%.<br />
<br />
As a result of their profound ability to degrade and live predominately on alkanes, as well as to become the dominant microbes in oil-contaminated areas, ''Alcanivorax'' plays a huge role in the biological cleanup of oil-contaminated environments. These oil-contaminated environments in the ocean are largely due to anthropogenic sources such as oil spills caused by tankers accidents (Figure 2), and cause serious ecological damage to plants and animals on the coast as well as other inhabitants of the ocean. Microbes such as ''Alcanivorax'' provide a major route for the breakdown of these pollutants, and demonstrate how marine bacteria keep the environment in check. Of all the ''Alcanivorax'' species and other oil-degrading microbes, ''Alcanivorax borkumensis'' is one of the most important worldwide due to the fact it produces a wide variety of very efficient oil-degrading enzymes. With this knowledge, ''A. borkumensis'' could provide a useful tool for bioremediation of oil spills.<br />
<br />
==Genome Structure==<br />
[[Image:Lorenzo.gif|thumbnail|200px|Figure 3. Mechanisms for oil degradation and survival encoded by the ''A. borkumensis SK2'' genome. Image from Victor de Lorenzo[http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html]]]<br />
<br />
The ''Alcanivorax borkumensis'' strain SK2, isolated from a seawater sediment sample in the North Sea at a site located near the Isle of Borkum, was the first hydrocarbonoclastic bacterium to be sequenced and was completed by Susanne Schneiker et al. It's genome consists of a single circular chromosome with 3,120,143 base pairs and an average G+C content of 54.7%. The genomic analysis of ''A. borkumensis SK2'' revealed several new insights into the bacterium's role for (i) n-alkane degradation (which includes metabolism, biosurfactant production and biofilm production), (ii) it's system for capturing or scavenging the small amounts of nitrogen, phosphorous, sulfur, and other elements in a nutrient-poor marine environment which allows for more efficient alkane degradation due to their main limitation of nutrient availability, (iii) as well as means for coping with stress factors such as high salt contents and high UV radiation since it thrives mostly in the upper layers in the ocean where UV light is encountered (Figure 3).<br />
<br />
It's genome encodes several systems for the catabolism of hydrocarbons which allow the bacertium to degrade all sorts of alkanes such as AlkB1 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C5-C12, and AlkB2 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C8 to C16. Both these systems are located close to the origin of replication of the chromosome. ''A. borkumensis'' is also able to degrade alkanes up to C32, branched aliphatic hydrocarbons, isoprenoid hydrocarbons such as phytane, as well as alkylarenes and alkylcycloalkanes. Thus, the genome encodes for a broad spectrum of systems for the catabolism of hydrocarbons, giving it a competitive advantage over other oil-degrading marine microbial communities. To deal with the damaging effects of UV light, ''A. borkumensis'' has a number of genes that reduce the damage. These include the full genes for DNA alkylation, recombinational and nucleotide excision repair, base excision repair, as well as the SOS response [4].<br />
<br />
==Cell Structure, Metabolism and Life Cycle==<br />
''Alcanivorax borkumensis'', a Gram-negative, rod-shaped chemoorganotroph, is able to use n-alkanes as its principle carbon and energy source by use of the broad spectrum of oil-degrading enzymes it possesses, but they can also use a limited number of organic compounds such as aliphatic hydrocarbons, volatile fatty acids, and pyruvate. However, it cannot utilize carbon sources such as sugars or amino acids. Cells grown with pyruvate were observed to be 2.0-3.0 micrometers in length and 0.4-07 micrometers in diameter, however, cells were shorter (1.0-1.5 micrometers in length) when cells were grown with n-alkanes as the carbon source (see Figure 1) [5]. When the slow growing ''A. borkumensis'' uses n-alkanes exclusively, the microbes produce extracellular and membrane-bound surface-active glucose lipids called biosurfactants. These biosurfactants reduce the surface tension of water from 72 to 29 mN m-1 and act as natural emulsifiers which enhances the break up of oil-in-water emulsions and prevent degradation of alkanes [4,5]. Due to the low solubility of oil in water, most oil degradation takes place at the oil-water interface where ''A. borkumensis'' attaches and forms a biofilm around the oil droplets as depicted in Figure 3.<br />
<br />
==Ecology==<br />
[[Image:OilContamination.jpg|thumbnail|200px|Figure 4. Oil spills in the ocean affect more than the aquatic environment. ''Alcanivorax'' helps reduce the damage on ecosystem health.]]<br />
<br />
''Alcanivorix'' is a novel species living in the oceans that plays a major role in keeping our pristine oceans as well as the inhabitants of the ocean and the inhabitants of the coastal regions in good health. It has been detected worldwide in places such as the Mediterranean Sea, Pacific Ocean, and the Arctic Sea [4]. In seawater with high concentrations of n-alkanes (as a result of oil spills, natural oil fields, and/or processing plants), ''Alcanivorax'' quickly becomes the predominant microbial community and is found in higher populations when compared to ''Alcanivorax'' in unpolluted seawater. There have been several recent fields studies on bacterial on community dynamics and hydrocarbon degradation in coastal areas contaminated with oil. These field studies have demonstrated the immense importance of ''Alcanivorax'' (particularly ''A. Borkumensis'') in oil-spill bioremediation [2].<br />
<br />
==References==<br />
[1] [http://ijs.sgmjournals.org/cgi/content/abstract/53/1/331 Fernandez-Martinez, Javier, Maria J. Pujalte, Jesus Garcia-Martinez, Manuel Mata, Esperanza Garay, and Francisco Rodriguez-Valera. "Description of ''Alcanivorax Venustensis'' sp. nov. and Reclassification of ''Fundibacter Jadensis'' DSM 12178T (Bruns and Berthe-Corti 1999) As ''Alcanivorax Jadensis'' comb. nov., Members of the Emended Genus ''Alcanivorax''." International Journal of Systematic and Evolutionary Microbiology 53 (2003): 331-338.]<br />
<br />
[2] [http://www.blackwell-synergy.com/doi/pdf/10.1046/j.1468-2920.2003.00468.x Hara, Akihiro, Kazuaki Syutsubo, and Shigeaki Harayama. "''Alcanivorax'' Which Prevails In Oil-contaminated Seawater Exhibits Broad Substrate Specificity For Alkane Degradation." Environmental Microbiology 5.9 (2003): 746-753.]<br />
<br />
[3] [http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html Lorenzo, Víctor De. "Blueprint of an Oil-eating Bacterium." Nature Biotechnology 24 (2006): 952-953.]<br />
<br />
[4] [http://www.ncbi.nlm.nih.gov/pubmed/16878126 Schneiker, S. et al. "Genome Sequence of the Ubiquitous Hydrocarbon-degrading Marine Bacterium A''lcanivorax Borkumensis''." Nature Biotechnology 24 (2006): 997-1004.]<br />
<br />
[5] [http://ijs.sgmjournals.org/cgi/content/abstract/48/2/339 Yakimov, Michail M., Peter N. Golyshin, Siegmund Lang, Edward R. B. Moore, Wolf-Rainer Abraham, Heinrich Lunsdorf, and Kenneth N. Timmis. "''Alcanivorax Borkumensis'' gen. nov., sp. nov., A New, Hydrocarbon-degrading And Surfactant-producing Marine Bacterium." International Journal of Systematic Bacteriology 48 (1998): 339-348.]<br />
<br />
==Author==<br />
Page authored by Andrew Buss, student of [http://www.kbs.msu.edu/faculty/lennon/ Prof. Jay Lennon] at Michigan State University.</div>Bussand1https://microbewiki.kenyon.edu/index.php?title=Alcanivorax&diff=29799Alcanivorax2008-04-11T15:48:34Z<p>Bussand1: /* Cell Structure, Metabolism and Life Cycle */</p>
<hr />
<div>[[Image:AlcVorax PROCARYOTES01b-1.JPG|thumbnail|300px|Figure 1. ''Alcanivorax borkumensis''. Image from Helmholtz Centre for Infection Research[http://www.helmholtz-hzi.de/en/news_public_relation/press_releases/view/article/complete/oil_tanker_accidents_as_a_source_of_food/]]]<br />
<br />
==Classification==<br />
<br />
'''Bacteria'''; Phylum: '''Proteobacteria'''; Class: '''Gammaproteobacteria'''; Order: '''Oceanospirillales'''; Family: '''Alcanivoracaceae'''<br />
<br />
===Species===<br />
<br />
{|<br />
| height="10" bgcolor="#FFDF95" |<br />
'''NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Tree&id=2&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy]'''<br />
|}<br />
<br />
*''Alcanivorax balearicum''<br />
*''Alcanivorax borkumensis''<br />
*''Alcanivorax dieselolei''<br />
*''Alcanivorax indicus''<br />
*''Alcanivorax jadensis''<br />
*''Alcanivorax venustensis''<br />
<br />
==Description and Significance==<br />
[[Image:PWS tanker oil spill.jpg|thumbnail|200px|Figure 2. Supertanker Exxon Valdez grounded on Bligh Reef which released 11 million gallons of crude oil into the water. This oil-contaminated seawater is the preferred habitat for ''Alcanivorax''. Image from USGS[http://menlocampus.wr.usgs.gov/50years/accomplishments/oil.html]]]<br />
<br />
''Alcanivorax'', first described in 1998, is a Gram-negative, halophilic, aerobic, rod-shaped, oil-degrading marine bacterium that is found in low abundances in unpolluted environments in the upper layers of the ocean, but quickly becomes the predominant microbe in oil-contaminated open oceans and coastal waters when nitrogen and phosphorus are not limiting [2]. When conditions in these moderately halophilic environments are right, ''Alcanivorax'' may make up 80-90% of the oil-degrading microbes present in the area [4]. It is described as a non-motile bactertium which is true for species such as ''Alcanivorax borkumensis'', but other species such as ''Alcanivorax venustensis'' were described to be motile by polar flagella [1]. The optimial conditions described for A.borkumensis growth include temperatures in the range of 20-30 degrees celsius, and a NaCl concentration of 3-10%.<br />
<br />
As a result of their profound ability to degrade and live predominately on alkanes, as well as to become the dominant microbes in oil-contaminated areas, ''Alcanivorax'' plays a huge role in the biological cleanup of oil-contaminated environments. These oil-contaminated environments in the ocean are largely due to anthropogenic sources such as oil spills caused by tankers accidents (Figure 2), and cause serious ecological damage to plants and animals on the coast as well as other inhabitants of the ocean. Microbes such as ''Alcanivorax'' provide a major route for the breakdown of these pollutants, and demonstrate how marine bacteria keep the environment in check. Of all the ''Alcanivorax'' species and other oil-degrading microbes, ''Alcanivorax borkumensis'' is one of the most important worldwide due to the fact it produces a wide variety of very efficient oil-degrading enzymes. With this knowledge, ''A. borkumensis'' could provide a useful tool for bioremediation of oil spills.<br />
<br />
==Genome Structure==<br />
[[Image:Lorenzo.gif|thumbnail|200px|Figure 3. Mechanisms for oil degradation and survival encoded by the ''A. borkumensis SK2'' genome. Image from Victor de Lorenzo[http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html]]]<br />
<br />
The ''Alcanivorax borkumensis'' strain SK2, isolated from a seawater sediment sample in the North Sea at a site located near the Isle of Borkum, was the first hydrocarbonoclastic bacterium to be sequenced and was completed by Susanne Schneiker et al. It's genome consists of a single circular chromosome with 3,120,143 base pairs and an average G+C content of 54.7%. The genomic analysis of ''A. borkumensis SK2'' revealed several new insights into the bacterium's role for (i) n-alkane degradation (which includes metabolism, biosurfactant production and biofilm production), (ii) it's system for capturing or scavenging the small amounts of nitrogen, phosphorous, sulfur, and other elements in a nutrient-poor marine environment which allows for more efficient alkane degradation due to their main limitation of nutrient availability, (iii) as well as means for coping with stress factors such as high salt contents and high UV radiation since it thrives mostly in the upper layers in the ocean where UV light is encountered (Figure 3).<br />
<br />
It's genome encodes several systems for the catabolism of hydrocarbons which allow the bacertium to degrade all sorts of alkanes such as AlkB1 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C5-C12, and AlkB2 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C8 to C16. Both these systems are located close to the origin of replication of the chromosome. ''A. borkumensis'' is also able to degrade alkanes up to C32, branched aliphatic hydrocarbons, isoprenoid hydrocarbons such as phytane, as well as alkylarenes and alkylcycloalkanes. Thus, the genome encodes for a broad spectrum of systems for the catabolism of hydrocarbons, giving it a competitive advantage over other oil-degrading marine microbial communities. To deal with the damaging effects of UV light, ''A. borkumensis'' has a number of genes that reduce the damage. These include the full genes for DNA alkylation, recombinational and nucleotide excision repair, base excision repair, as well as the SOS response [4].<br />
<br />
==Cell Structure, Metabolism and Life Cycle==<br />
''Alcanivorax borkumensis'', a Gram-negative, rod-shaped chemoorganotroph, is able to use n-alkanes as its principle carbon and energy source by use of the broad spectrum of oil-degrading enzymes it possesses, but they can also use a limited number of organic compounds such as aliphatic hydrocarbons, volatile fatty acids, and pyruvate. However, it cannot utilize carbon sources such as sugars or amino acids. Cells grown with pyruvate were observed to be 2.0-3.0 micrometers in length and 0.4-07 micrometers in diameter, however, cells were shorter (1.0-1.5 micrometers in length) when cells were grown with n-alkanes as the carbon source (see Figure 1) [5]. When the slow growing ''A. borkumensis'' uses n-alkanes exclusively, the microbes produce extracellular and membrane-bound surface-active glucose lipids called biosurfactants. These biosurfactants reduce the surface tension of water from 72 to 29 mN m-1 and act as natural emulsifiers which enhances the break up of oil-in-water emulsions and prevent degradation of alkanes [4,5]. Due to the low solubility of oil in water, most oil degradation takes place at the oil-water interface where ''A. borkumensis'' attaches and forms a biofilm around the oil droplets as depicted in Figure 3.<br />
<br />
==Ecology==<br />
[[Image:OilContamination.jpg|thumbnail|200px|Figure 4. Oil spills in the ocean affect more than the aquatic environment. ''Alcanivorax'' helps reduce the damage on ecosystem health.]]<br />
<br />
''Alcanivorix'' is a novel species living in the oceans that plays a major role in keeping our pristine oceans as well as the inhabitants of the ocean and the inhabitants of the coastal regions in good health. It has been detected worldwide in places such as the Mediterranean Sea, Pacific Ocean, and the Arctic Sea [4]. In seawater with high concentrations of n-alkanes (as a result of oil spills, natural oil fields, and/or processing plants), ''Alkanivorax'' quickly becomes the predominant microbial community and is found in higher populations when compared to ''Alkanivorax'' in unpolluted seawater. There have been several recent fields studies on bacterial on community dynamics and hydrocarbon degradation in coastal areas contaminated with oil. These field studies have demonstrated the immense importance of ''Alcanivorax'' (particularly ''A. Borkumensis'') in oil-spill bioremediation [2].<br />
<br />
==References==<br />
[1] [http://ijs.sgmjournals.org/cgi/content/abstract/53/1/331 Fernandez-Martinez, Javier, Maria J. Pujalte, Jesus Garcia-Martinez, Manuel Mata, Esperanza Garay, and Francisco Rodriguez-Valera. "Description of ''Alcanivorax Venustensis'' sp. nov. and Reclassification of ''Fundibacter Jadensis'' DSM 12178T (Bruns and Berthe-Corti 1999) As ''Alcanivorax Jadensis'' comb. nov., Members of the Emended Genus ''Alcanivorax''." International Journal of Systematic and Evolutionary Microbiology 53 (2003): 331-338.]<br />
<br />
[2] [http://www.blackwell-synergy.com/doi/pdf/10.1046/j.1468-2920.2003.00468.x Hara, Akihiro, Kazuaki Syutsubo, and Shigeaki Harayama. "''Alcanivorax'' Which Prevails In Oil-contaminated Seawater Exhibits Broad Substrate Specificity For Alkane Degradation." Environmental Microbiology 5.9 (2003): 746-753.]<br />
<br />
[3] [http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html Lorenzo, Víctor De. "Blueprint of an Oil-eating Bacterium." Nature Biotechnology 24 (2006): 952-953.]<br />
<br />
[4] [http://www.ncbi.nlm.nih.gov/pubmed/16878126 Schneiker, S. et al. "Genome Sequence of the Ubiquitous Hydrocarbon-degrading Marine Bacterium A''lcanivorax Borkumensis''." Nature Biotechnology 24 (2006): 997-1004.]<br />
<br />
[5] [http://ijs.sgmjournals.org/cgi/content/abstract/48/2/339 Yakimov, Michail M., Peter N. Golyshin, Siegmund Lang, Edward R. B. Moore, Wolf-Rainer Abraham, Heinrich Lunsdorf, and Kenneth N. Timmis. "''Alcanivorax Borkumensis'' gen. nov., sp. nov., A New, Hydrocarbon-degrading And Surfactant-producing Marine Bacterium." International Journal of Systematic Bacteriology 48 (1998): 339-348.]<br />
<br />
==Author==<br />
Page authored by Andrew Buss, student of [http://www.kbs.msu.edu/faculty/lennon/ Prof. Jay Lennon] at Michigan State University.</div>Bussand1https://microbewiki.kenyon.edu/index.php?title=Alcanivorax&diff=29785Alcanivorax2008-04-10T03:03:20Z<p>Bussand1: </p>
<hr />
<div>[[Image:AlcVorax PROCARYOTES01b-1.JPG|thumbnail|300px|Figure 1. ''Alcanivorax borkumensis''. Image from Helmholtz Centre for Infection Research[http://www.helmholtz-hzi.de/en/news_public_relation/press_releases/view/article/complete/oil_tanker_accidents_as_a_source_of_food/]]]<br />
<br />
==Classification==<br />
<br />
'''Bacteria'''; Phylum: '''Proteobacteria'''; Class: '''Gammaproteobacteria'''; Order: '''Oceanospirillales'''; Family: '''Alcanivoracaceae'''<br />
<br />
===Species===<br />
<br />
{|<br />
| height="10" bgcolor="#FFDF95" |<br />
'''NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Tree&id=2&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy]'''<br />
|}<br />
<br />
*''Alcanivorax balearicum''<br />
*''Alcanivorax borkumensis''<br />
*''Alcanivorax dieselolei''<br />
*''Alcanivorax indicus''<br />
*''Alcanivorax jadensis''<br />
*''Alcanivorax venustensis''<br />
<br />
==Description and Significance==<br />
[[Image:PWS tanker oil spill.jpg|thumbnail|200px|Figure 2. Supertanker Exxon Valdez grounded on Bligh Reef which released 11 million gallons of crude oil into the water. This oil-contaminated seawater is the preferred habitat for ''Alcanivorax''. Image from USGS[http://menlocampus.wr.usgs.gov/50years/accomplishments/oil.html]]]<br />
<br />
''Alcanivorax'', first described in 1998, is a Gram-negative, halophilic, aerobic, rod-shaped, oil-degrading marine bacterium that is found in low abundances in unpolluted environments in the upper layers of the ocean, but quickly becomes the predominant microbe in oil-contaminated open oceans and coastal waters when nitrogen and phosphorus are not limiting [2]. When conditions in these moderately halophilic environments are right, ''Alcanivorax'' may make up 80-90% of the oil-degrading microbes present in the area [4]. It is described as a non-motile bactertium which is true for species such as ''Alcanivorax borkumensis'', but other species such as ''Alcanivorax venustensis'' were described to be motile by polar flagella [1]. The optimial conditions described for A.borkumensis growth include temperatures in the range of 20-30 degrees celsius, and a NaCl concentration of 3-10%.<br />
<br />
As a result of their profound ability to degrade and live predominately on alkanes, as well as to become the dominant microbes in oil-contaminated areas, ''Alcanivorax'' plays a huge role in the biological cleanup of oil-contaminated environments. These oil-contaminated environments in the ocean are largely due to anthropogenic sources such as oil spills caused by tankers accidents (Figure 2), and cause serious ecological damage to plants and animals on the coast as well as other inhabitants of the ocean. Microbes such as ''Alcanivorax'' provide a major route for the breakdown of these pollutants, and demonstrate how marine bacteria keep the environment in check. Of all the ''Alcanivorax'' species and other oil-degrading microbes, ''Alcanivorax borkumensis'' is one of the most important worldwide due to the fact it produces a wide variety of very efficient oil-degrading enzymes. With this knowledge, ''A. borkumensis'' could provide a useful tool for bioremediation of oil spills.<br />
<br />
==Genome Structure==<br />
[[Image:Lorenzo.gif|thumbnail|200px|Figure 3. Mechanisms for oil degradation and survival encoded by the ''A. borkumensis SK2'' genome. Image from Victor de Lorenzo[http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html]]]<br />
<br />
The ''Alcanivorax borkumensis'' strain SK2, isolated from a seawater sediment sample in the North Sea at a site located near the Isle of Borkum, was the first hydrocarbonoclastic bacterium to be sequenced and was completed by Susanne Schneiker et al. It's genome consists of a single circular chromosome with 3,120,143 base pairs and an average G+C content of 54.7%. The genomic analysis of ''A. borkumensis SK2'' revealed several new insights into the bacterium's role for (i) n-alkane degradation (which includes metabolism, biosurfactant production and biofilm production), (ii) it's system for capturing or scavenging the small amounts of nitrogen, phosphorous, sulfur, and other elements in a nutrient-poor marine environment which allows for more efficient alkane degradation due to their main limitation of nutrient availability, (iii) as well as means for coping with stress factors such as high salt contents and high UV radiation since it thrives mostly in the upper layers in the ocean where UV light is encountered (Figure 3).<br />
<br />
It's genome encodes several systems for the catabolism of hydrocarbons which allow the bacertium to degrade all sorts of alkanes such as AlkB1 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C5-C12, and AlkB2 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C8 to C16. Both these systems are located close to the origin of replication of the chromosome. ''A. borkumensis'' is also able to degrade alkanes up to C32, branched aliphatic hydrocarbons, isoprenoid hydrocarbons such as phytane, as well as alkylarenes and alkylcycloalkanes. Thus, the genome encodes for a broad spectrum of systems for the catabolism of hydrocarbons, giving it a competitive advantage over other oil-degrading marine microbial communities. To deal with the damaging effects of UV light, ''A. borkumensis'' has a number of genes that reduce the damage. These include the full genes for DNA alkylation, recombinational and nucleotide excision repair, base excision repair, as well as the SOS response [4].<br />
<br />
==Cell Structure, Metabolism and Life Cycle==<br />
''Alcanivorax borkumensis'', a Gram-negative, rod-shaped chemoorganotroph, is able to use n-alkanes as its principle carbon and energy source by use of the broad spectrum of oil-degrading enzymes it possesses, but they can also use a limited number of organic compounds such as aliphatic hydrocarbons, volatile fatty acids, and pyruvate. However, it cannot utilize carbon sources such as sugars or amino acids. Cells grown with pyruvate were observed to be 2.0-3.0 micrometers in length and 0.4-07 micrometers in diameter, however, cells were shorter (1.0-1.5 micrometers in length) when cells were grown with n-alkanes as the carbon source [5]. When the slow growing ''A. borkumensis'' uses n-alkanes exclusively, the microbes produce extracellular and membrane-bound surface-active glucose lipids called biosurfactants. These biosurfactants reduce the surface tension of water from 72 to 29 mN m-1 and act as natural emulsifiers which enhances the break up of oil-in-water emulsions and prevent degradation of alkanes [4,5]. Due to the low solubility of oil in water, most oil degradation takes place at the oil-water interface where ''A. borkumensis'' attaches and forms a biofilm around the oil droplets as depicted in Figure 3.<br />
<br />
==Ecology==<br />
[[Image:OilContamination.jpg|thumbnail|200px|Figure 4. Oil spills in the ocean affect more than the aquatic environment. ''Alcanivorax'' helps reduce the damage on ecosystem health.]]<br />
<br />
''Alcanivorix'' is a novel species living in the oceans that plays a major role in keeping our pristine oceans as well as the inhabitants of the ocean and the inhabitants of the coastal regions in good health. It has been detected worldwide in places such as the Mediterranean Sea, Pacific Ocean, and the Arctic Sea [4]. In seawater with high concentrations of n-alkanes (as a result of oil spills, natural oil fields, and/or processing plants), ''Alkanivorax'' quickly becomes the predominant microbial community and is found in higher populations when compared to ''Alkanivorax'' in unpolluted seawater. There have been several recent fields studies on bacterial on community dynamics and hydrocarbon degradation in coastal areas contaminated with oil. These field studies have demonstrated the immense importance of ''Alcanivorax'' (particularly ''A. Borkumensis'') in oil-spill bioremediation [2].<br />
<br />
==References==<br />
[1] [http://ijs.sgmjournals.org/cgi/content/abstract/53/1/331 Fernandez-Martinez, Javier, Maria J. Pujalte, Jesus Garcia-Martinez, Manuel Mata, Esperanza Garay, and Francisco Rodriguez-Valera. "Description of ''Alcanivorax Venustensis'' sp. nov. and Reclassification of ''Fundibacter Jadensis'' DSM 12178T (Bruns and Berthe-Corti 1999) As ''Alcanivorax Jadensis'' comb. nov., Members of the Emended Genus ''Alcanivorax''." International Journal of Systematic and Evolutionary Microbiology 53 (2003): 331-338.]<br />
<br />
[2] [http://www.blackwell-synergy.com/doi/pdf/10.1046/j.1468-2920.2003.00468.x Hara, Akihiro, Kazuaki Syutsubo, and Shigeaki Harayama. "''Alcanivorax'' Which Prevails In Oil-contaminated Seawater Exhibits Broad Substrate Specificity For Alkane Degradation." Environmental Microbiology 5.9 (2003): 746-753.]<br />
<br />
[3] [http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html Lorenzo, Víctor De. "Blueprint of an Oil-eating Bacterium." Nature Biotechnology 24 (2006): 952-953.]<br />
<br />
[4] [http://www.ncbi.nlm.nih.gov/pubmed/16878126 Schneiker, S. et al. "Genome Sequence of the Ubiquitous Hydrocarbon-degrading Marine Bacterium A''lcanivorax Borkumensis''." Nature Biotechnology 24 (2006): 997-1004.]<br />
<br />
[5] [http://ijs.sgmjournals.org/cgi/content/abstract/48/2/339 Yakimov, Michail M., Peter N. Golyshin, Siegmund Lang, Edward R. B. Moore, Wolf-Rainer Abraham, Heinrich Lunsdorf, and Kenneth N. Timmis. "''Alcanivorax Borkumensis'' gen. nov., sp. nov., A New, Hydrocarbon-degrading And Surfactant-producing Marine Bacterium." International Journal of Systematic Bacteriology 48 (1998): 339-348.]<br />
<br />
==Author==<br />
Page authored by Andrew Buss, student of [http://www.kbs.msu.edu/faculty/lennon/ Prof. Jay Lennon] at Michigan State University.</div>Bussand1https://microbewiki.kenyon.edu/index.php?title=File:OilContamination.jpg&diff=29784File:OilContamination.jpg2008-04-10T02:58:19Z<p>Bussand1: Oil Spills in the oceans don't only affect the aquatic environments.</p>
<hr />
<div>Oil Spills in the oceans don't only affect the aquatic environments.</div>Bussand1https://microbewiki.kenyon.edu/index.php?title=Alcanivorax&diff=29783Alcanivorax2008-04-10T02:56:02Z<p>Bussand1: /* Ecology */</p>
<hr />
<div>[[Image:AlcVorax PROCARYOTES01b-1.JPG|thumbnail|300px|Figure 1. ''Alcanivorax borkumensis''. Image from Helmholtz Centre for Infection Research[http://www.helmholtz-hzi.de/en/news_public_relation/press_releases/view/article/complete/oil_tanker_accidents_as_a_source_of_food/]]]<br />
<br />
==Classification==<br />
<br />
'''Bacteria'''; Phylum: '''Proteobacteria'''; Class: '''Gammaproteobacteria'''; Order: '''Oceanospirillales'''; Family: '''Alcanivoracaceae'''<br />
<br />
===Species===<br />
<br />
{|<br />
| height="10" bgcolor="#FFDF95" |<br />
'''NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Tree&id=2&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy]'''<br />
|}<br />
<br />
*''Alcanivorax balearicum''<br />
*''Alcanivorax borkumensis''<br />
*''Alcanivorax dieselolei''<br />
*''Alcanivorax indicus''<br />
*''Alcanivorax jadensis''<br />
*''Alcanivorax venustensis''<br />
<br />
==Description and Significance==<br />
[[Image:PWS tanker oil spill.jpg|thumbnail|200px|Figure 2. Supertanker Exxon Valdez grounded on Bligh Reef which released 11 million gallons of crude oil into the water. This oil-contaminated seawater is the preferred habitat for ''Alcanivorax''. Image from USGS[http://menlocampus.wr.usgs.gov/50years/accomplishments/oil.html]]]<br />
<br />
''Alcanivorax'', first described in 1998, is a Gram-negative, halophilic, aerobic, rod-shaped, oil-degrading marine bacterium that is found in low abundances in unpolluted environments in the upper layers of the ocean, but quickly becomes the predominant microbe in oil-contaminated open oceans and coastal waters when nitrogen and phosphorus are not limiting [2]. When conditions in these moderately halophilic environments are right, ''Alcanivorax'' may make up 80-90% of the oil-degrading microbes present in the area [4]. It is described as a non-motile bactertium which is true for species such as ''Alcanivorax borkumensis'', but other species such as ''Alcanivorax venustensis'' were described to be motile by polar flagella [1]. The optimial conditions described for A.borkumensis growth include temperatures in the range of 20-30 degrees celsius, and a NaCl concentration of 3-10%.<br />
<br />
As a result of their profound ability to degrade and live predominately on alkanes, as well as to become the dominant microbes in oil-contaminated areas, ''Alcanivorax'' plays a huge role in the biological cleanup of oil-contaminated environments. These oil-contaminated environments in the ocean are largely due to anthropogenic sources such as oil spills caused by tankers accidents (Figure 2), and cause serious ecological damage to plants and animals on the coast as well as other inhabitants of the ocean. Microbes such as ''Alcanivorax'' provide a major route for the breakdown of these pollutants, and demonstrate how marine bacteria keep the environment in check. Of all the ''Alcanivorax'' species and other oil-degrading microbes, ''Alcanivorax borkumensis'' is one of the most important worldwide due to the fact it produces a wide variety of very efficient oil-degrading enzymes. With this knowledge, ''A. borkumensis'' could provide a useful tool for bioremediation of oil spills.<br />
<br />
==Genome Structure==<br />
[[Image:Lorenzo.gif|thumbnail|200px|Figure 3. Mechanisms for oil degradation and survival encoded by the ''A. borkumensis SK2'' genome. Image from Victor de Lorenzo[http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html]]]<br />
<br />
The ''Alcanivorax borkumensis'' strain SK2, isolated from a seawater sediment sample in the North Sea at a site located near the Isle of Borkum, was the first hydrocarbonoclastic bacterium to be sequenced and was completed by Susanne Schneiker et al. It's genome consists of a single circular chromosome with 3,120,143 base pairs and an average G+C content of 54.7%. The genomic analysis of ''A. borkumensis SK2'' revealed several new insights into the bacterium's role for (i) n-alkane degradation (which includes metabolism, biosurfactant production and biofilm production), (ii) it's system for capturing or scavenging the small amounts of nitrogen, phosphorous, sulfur, and other elements in a nutrient-poor marine environment which allows for more efficient alkane degradation due to their main limitation of nutrient availability, (iii) as well as means for coping with stress factors such as high salt contents and high UV radiation since it thrives mostly in the upper layers in the ocean where UV light is encountered (Figure 3).<br />
<br />
It's genome encodes several systems for the catabolism of hydrocarbons which allow the bacertium to degrade all sorts of alkanes such as AlkB1 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C5-C12, and AlkB2 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C8 to C16. Both these systems are located close to the origin of replication of the chromosome. ''A. borkumensis'' is also able to degrade alkanes up to C32, branched aliphatic hydrocarbons, isoprenoid hydrocarbons such as phytane, as well as alkylarenes and alkylcycloalkanes. Thus, the genome encodes for a broad spectrum of systems for the catabolism of hydrocarbons, giving it a competitive advantage over other oil-degrading marine microbial communities. To deal with the damaging effects of UV light, ''A. borkumensis'' has a number of genes that reduce the damage. These include the full genes for DNA alkylation, recombinational and nucleotide excision repair, base excision repair, as well as the SOS response [4].<br />
<br />
==Cell Structure, Metabolism and Life Cycle==<br />
''Alcanivorax borkumensis'', a Gram-negative, rod-shaped chemoorganotroph, is able to use n-alkanes as its principle carbon and energy source by use of the broad spectrum of oil-degrading enzymes it possesses, but they can also use a limited number of organic compounds such as aliphatic hydrocarbons, volatile fatty acids, and pyruvate. However, it cannot utilize carbon sources such as sugars or amino acids. Cells grown with pyruvate were observed to be 2.0-3.0 micrometers in length and 0.4-07 micrometers in diameter, however, cells were shorter (1.0-1.5 micrometers in length) when cells were grown with n-alkanes as the carbon source [5]. When the slow growing ''A. borkumensis'' uses n-alkanes exclusively, the microbes produce extracellular and membrane-bound surface-active glucose lipids called biosurfactants. These biosurfactants reduce the surface tension of water from 72 to 29 mN m-1 and act as natural emulsifiers which enhances the break up of oil-in-water emulsions and prevent degradation of alkanes [4,5]. Due to the low solubility of oil in water, most oil degradation takes place at the oil-water interface where ''A. borkumensis'' attaches and forms a biofilm around the oil droplets as depicted in Figure 3.<br />
<br />
==Ecology==<br />
''Alcanivorix'' is a novel species living in the oceans that plays a major role in keeping our pristine oceans as well as the inhabitants of the ocean and the inhabitants of the coastal regions in good health. It has been detected worldwide in places such as the Mediterranean Sea, Pacific Ocean, and the Arctic Sea [4]. In seawater with high concentrations of n-alkanes (as a result of oil spills, natural oil fields, and/or processing plants), ''Alkanivorax'' quickly becomes the predominant microbial community and is found in higher populations when compared to ''Alkanivorax'' in unpolluted seawater. There have been several recent fields studies on bacterial on community dynamics and hydrocarbon degradation in coastal areas contaminated with oil. These field studies have demonstrated the immense importance of ''Alcanivorax'' (particularly ''A. Borkumensis'') in oil-spill bioremediation [2].<br />
<br />
==References==<br />
[1] [http://ijs.sgmjournals.org/cgi/content/abstract/53/1/331 Fernandez-Martinez, Javier, Maria J. Pujalte, Jesus Garcia-Martinez, Manuel Mata, Esperanza Garay, and Francisco Rodriguez-Valera. "Description of ''Alcanivorax Venustensis'' sp. nov. and Reclassification of ''Fundibacter Jadensis'' DSM 12178T (Bruns and Berthe-Corti 1999) As ''Alcanivorax Jadensis'' comb. nov., Members of the Emended Genus ''Alcanivorax''." International Journal of Systematic and Evolutionary Microbiology 53 (2003): 331-338.]<br />
<br />
[2] [http://www.blackwell-synergy.com/doi/pdf/10.1046/j.1468-2920.2003.00468.x Hara, Akihiro, Kazuaki Syutsubo, and Shigeaki Harayama. "''Alcanivorax'' Which Prevails In Oil-contaminated Seawater Exhibits Broad Substrate Specificity For Alkane Degradation." Environmental Microbiology 5.9 (2003): 746-753.]<br />
<br />
[3] [http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html Lorenzo, Víctor De. "Blueprint of an Oil-eating Bacterium." Nature Biotechnology 24 (2006): 952-953.]<br />
<br />
[4] [http://www.ncbi.nlm.nih.gov/pubmed/16878126 Schneiker, S. et al. "Genome Sequence of the Ubiquitous Hydrocarbon-degrading Marine Bacterium A''lcanivorax Borkumensis''." Nature Biotechnology 24 (2006): 997-1004.]<br />
<br />
[5] [http://ijs.sgmjournals.org/cgi/content/abstract/48/2/339 Yakimov, Michail M., Peter N. Golyshin, Siegmund Lang, Edward R. B. Moore, Wolf-Rainer Abraham, Heinrich Lunsdorf, and Kenneth N. Timmis. "''Alcanivorax Borkumensis'' gen. nov., sp. nov., A New, Hydrocarbon-degrading And Surfactant-producing Marine Bacterium." International Journal of Systematic Bacteriology 48 (1998): 339-348.]<br />
<br />
==Author==<br />
Page authored by Andrew Buss, student of [http://www.kbs.msu.edu/faculty/lennon/ Prof. Jay Lennon] at Michigan State University.</div>Bussand1https://microbewiki.kenyon.edu/index.php?title=Alcanivorax&diff=29782Alcanivorax2008-04-10T02:48:13Z<p>Bussand1: /* Ecology */</p>
<hr />
<div>[[Image:AlcVorax PROCARYOTES01b-1.JPG|thumbnail|300px|Figure 1. ''Alcanivorax borkumensis''. Image from Helmholtz Centre for Infection Research[http://www.helmholtz-hzi.de/en/news_public_relation/press_releases/view/article/complete/oil_tanker_accidents_as_a_source_of_food/]]]<br />
<br />
==Classification==<br />
<br />
'''Bacteria'''; Phylum: '''Proteobacteria'''; Class: '''Gammaproteobacteria'''; Order: '''Oceanospirillales'''; Family: '''Alcanivoracaceae'''<br />
<br />
===Species===<br />
<br />
{|<br />
| height="10" bgcolor="#FFDF95" |<br />
'''NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Tree&id=2&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy]'''<br />
|}<br />
<br />
*''Alcanivorax balearicum''<br />
*''Alcanivorax borkumensis''<br />
*''Alcanivorax dieselolei''<br />
*''Alcanivorax indicus''<br />
*''Alcanivorax jadensis''<br />
*''Alcanivorax venustensis''<br />
<br />
==Description and Significance==<br />
[[Image:PWS tanker oil spill.jpg|thumbnail|200px|Figure 2. Supertanker Exxon Valdez grounded on Bligh Reef which released 11 million gallons of crude oil into the water. This oil-contaminated seawater is the preferred habitat for ''Alcanivorax''. Image from USGS[http://menlocampus.wr.usgs.gov/50years/accomplishments/oil.html]]]<br />
<br />
''Alcanivorax'', first described in 1998, is a Gram-negative, halophilic, aerobic, rod-shaped, oil-degrading marine bacterium that is found in low abundances in unpolluted environments in the upper layers of the ocean, but quickly becomes the predominant microbe in oil-contaminated open oceans and coastal waters when nitrogen and phosphorus are not limiting [2]. When conditions in these moderately halophilic environments are right, ''Alcanivorax'' may make up 80-90% of the oil-degrading microbes present in the area [4]. It is described as a non-motile bactertium which is true for species such as ''Alcanivorax borkumensis'', but other species such as ''Alcanivorax venustensis'' were described to be motile by polar flagella [1]. The optimial conditions described for A.borkumensis growth include temperatures in the range of 20-30 degrees celsius, and a NaCl concentration of 3-10%.<br />
<br />
As a result of their profound ability to degrade and live predominately on alkanes, as well as to become the dominant microbes in oil-contaminated areas, ''Alcanivorax'' plays a huge role in the biological cleanup of oil-contaminated environments. These oil-contaminated environments in the ocean are largely due to anthropogenic sources such as oil spills caused by tankers accidents (Figure 2), and cause serious ecological damage to plants and animals on the coast as well as other inhabitants of the ocean. Microbes such as ''Alcanivorax'' provide a major route for the breakdown of these pollutants, and demonstrate how marine bacteria keep the environment in check. Of all the ''Alcanivorax'' species and other oil-degrading microbes, ''Alcanivorax borkumensis'' is one of the most important worldwide due to the fact it produces a wide variety of very efficient oil-degrading enzymes. With this knowledge, ''A. borkumensis'' could provide a useful tool for bioremediation of oil spills.<br />
<br />
==Genome Structure==<br />
[[Image:Lorenzo.gif|thumbnail|200px|Figure 3. Mechanisms for oil degradation and survival encoded by the ''A. borkumensis SK2'' genome. Image from Victor de Lorenzo[http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html]]]<br />
<br />
The ''Alcanivorax borkumensis'' strain SK2, isolated from a seawater sediment sample in the North Sea at a site located near the Isle of Borkum, was the first hydrocarbonoclastic bacterium to be sequenced and was completed by Susanne Schneiker et al. It's genome consists of a single circular chromosome with 3,120,143 base pairs and an average G+C content of 54.7%. The genomic analysis of ''A. borkumensis SK2'' revealed several new insights into the bacterium's role for (i) n-alkane degradation (which includes metabolism, biosurfactant production and biofilm production), (ii) it's system for capturing or scavenging the small amounts of nitrogen, phosphorous, sulfur, and other elements in a nutrient-poor marine environment which allows for more efficient alkane degradation due to their main limitation of nutrient availability, (iii) as well as means for coping with stress factors such as high salt contents and high UV radiation since it thrives mostly in the upper layers in the ocean where UV light is encountered (Figure 3).<br />
<br />
It's genome encodes several systems for the catabolism of hydrocarbons which allow the bacertium to degrade all sorts of alkanes such as AlkB1 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C5-C12, and AlkB2 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C8 to C16. Both these systems are located close to the origin of replication of the chromosome. ''A. borkumensis'' is also able to degrade alkanes up to C32, branched aliphatic hydrocarbons, isoprenoid hydrocarbons such as phytane, as well as alkylarenes and alkylcycloalkanes. Thus, the genome encodes for a broad spectrum of systems for the catabolism of hydrocarbons, giving it a competitive advantage over other oil-degrading marine microbial communities. To deal with the damaging effects of UV light, ''A. borkumensis'' has a number of genes that reduce the damage. These include the full genes for DNA alkylation, recombinational and nucleotide excision repair, base excision repair, as well as the SOS response [4].<br />
<br />
==Cell Structure, Metabolism and Life Cycle==<br />
''Alcanivorax borkumensis'', a Gram-negative, rod-shaped chemoorganotroph, is able to use n-alkanes as its principle carbon and energy source by use of the broad spectrum of oil-degrading enzymes it possesses, but they can also use a limited number of organic compounds such as aliphatic hydrocarbons, volatile fatty acids, and pyruvate. However, it cannot utilize carbon sources such as sugars or amino acids. Cells grown with pyruvate were observed to be 2.0-3.0 micrometers in length and 0.4-07 micrometers in diameter, however, cells were shorter (1.0-1.5 micrometers in length) when cells were grown with n-alkanes as the carbon source [5]. When the slow growing ''A. borkumensis'' uses n-alkanes exclusively, the microbes produce extracellular and membrane-bound surface-active glucose lipids called biosurfactants. These biosurfactants reduce the surface tension of water from 72 to 29 mN m-1 and act as natural emulsifiers which enhances the break up of oil-in-water emulsions and prevent degradation of alkanes [4,5]. Due to the low solubility of oil in water, most oil degradation takes place at the oil-water interface where ''A. borkumensis'' attaches and forms a biofilm around the oil droplets as depicted in Figure 3.<br />
<br />
==Ecology==<br />
''Alcanivorix'' is a novel species living in the oceans that plays a major role in keeping our pristine oceans in good condition as well as the inhabitants of the ocean and the inhabitants of the coastal regions. It has been detected worldwide in places such as the Mediterranean Sea, Pacific Ocean, and the Arctic Sea [4]. In seawater with high concentrations of n-alkanes (as a result of oil spills, natural oil fields, and/or processing plants), ''Alkanivorax'' quickly becomes the predominant microbial community and is found in higher populations when compared to ''Alkanivorax'' in unpolluted seawater. There have been several recent fields studies on bacterial on community dynamics and hydrocarbon degradation in coastal areas contaminated with oil. These field studies have demonstrated the immense importance of ''Alcanivorax'' (particularly ''A. Borkumensis'') in oil-spill bioremediation [2].<br />
<br />
==References==<br />
[1] [http://ijs.sgmjournals.org/cgi/content/abstract/53/1/331 Fernandez-Martinez, Javier, Maria J. Pujalte, Jesus Garcia-Martinez, Manuel Mata, Esperanza Garay, and Francisco Rodriguez-Valera. "Description of ''Alcanivorax Venustensis'' sp. nov. and Reclassification of ''Fundibacter Jadensis'' DSM 12178T (Bruns and Berthe-Corti 1999) As ''Alcanivorax Jadensis'' comb. nov., Members of the Emended Genus ''Alcanivorax''." International Journal of Systematic and Evolutionary Microbiology 53 (2003): 331-338.]<br />
<br />
[2] [http://www.blackwell-synergy.com/doi/pdf/10.1046/j.1468-2920.2003.00468.x Hara, Akihiro, Kazuaki Syutsubo, and Shigeaki Harayama. "''Alcanivorax'' Which Prevails In Oil-contaminated Seawater Exhibits Broad Substrate Specificity For Alkane Degradation." Environmental Microbiology 5.9 (2003): 746-753.]<br />
<br />
[3] [http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html Lorenzo, Víctor De. "Blueprint of an Oil-eating Bacterium." Nature Biotechnology 24 (2006): 952-953.]<br />
<br />
[4] [http://www.ncbi.nlm.nih.gov/pubmed/16878126 Schneiker, S. et al. "Genome Sequence of the Ubiquitous Hydrocarbon-degrading Marine Bacterium A''lcanivorax Borkumensis''." Nature Biotechnology 24 (2006): 997-1004.]<br />
<br />
[5] [http://ijs.sgmjournals.org/cgi/content/abstract/48/2/339 Yakimov, Michail M., Peter N. Golyshin, Siegmund Lang, Edward R. B. Moore, Wolf-Rainer Abraham, Heinrich Lunsdorf, and Kenneth N. Timmis. "''Alcanivorax Borkumensis'' gen. nov., sp. nov., A New, Hydrocarbon-degrading And Surfactant-producing Marine Bacterium." International Journal of Systematic Bacteriology 48 (1998): 339-348.]<br />
<br />
==Author==<br />
Page authored by Andrew Buss, student of [http://www.kbs.msu.edu/faculty/lennon/ Prof. Jay Lennon] at Michigan State University.</div>Bussand1https://microbewiki.kenyon.edu/index.php?title=Alcanivorax&diff=29781Alcanivorax2008-04-10T02:47:42Z<p>Bussand1: /* Ecology and Pathogenesis */</p>
<hr />
<div>[[Image:AlcVorax PROCARYOTES01b-1.JPG|thumbnail|300px|Figure 1. ''Alcanivorax borkumensis''. Image from Helmholtz Centre for Infection Research[http://www.helmholtz-hzi.de/en/news_public_relation/press_releases/view/article/complete/oil_tanker_accidents_as_a_source_of_food/]]]<br />
<br />
==Classification==<br />
<br />
'''Bacteria'''; Phylum: '''Proteobacteria'''; Class: '''Gammaproteobacteria'''; Order: '''Oceanospirillales'''; Family: '''Alcanivoracaceae'''<br />
<br />
===Species===<br />
<br />
{|<br />
| height="10" bgcolor="#FFDF95" |<br />
'''NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Tree&id=2&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy]'''<br />
|}<br />
<br />
*''Alcanivorax balearicum''<br />
*''Alcanivorax borkumensis''<br />
*''Alcanivorax dieselolei''<br />
*''Alcanivorax indicus''<br />
*''Alcanivorax jadensis''<br />
*''Alcanivorax venustensis''<br />
<br />
==Description and Significance==<br />
[[Image:PWS tanker oil spill.jpg|thumbnail|200px|Figure 2. Supertanker Exxon Valdez grounded on Bligh Reef which released 11 million gallons of crude oil into the water. This oil-contaminated seawater is the preferred habitat for ''Alcanivorax''. Image from USGS[http://menlocampus.wr.usgs.gov/50years/accomplishments/oil.html]]]<br />
<br />
''Alcanivorax'', first described in 1998, is a Gram-negative, halophilic, aerobic, rod-shaped, oil-degrading marine bacterium that is found in low abundances in unpolluted environments in the upper layers of the ocean, but quickly becomes the predominant microbe in oil-contaminated open oceans and coastal waters when nitrogen and phosphorus are not limiting [2]. When conditions in these moderately halophilic environments are right, ''Alcanivorax'' may make up 80-90% of the oil-degrading microbes present in the area [4]. It is described as a non-motile bactertium which is true for species such as ''Alcanivorax borkumensis'', but other species such as ''Alcanivorax venustensis'' were described to be motile by polar flagella [1]. The optimial conditions described for A.borkumensis growth include temperatures in the range of 20-30 degrees celsius, and a NaCl concentration of 3-10%.<br />
<br />
As a result of their profound ability to degrade and live predominately on alkanes, as well as to become the dominant microbes in oil-contaminated areas, ''Alcanivorax'' plays a huge role in the biological cleanup of oil-contaminated environments. These oil-contaminated environments in the ocean are largely due to anthropogenic sources such as oil spills caused by tankers accidents (Figure 2), and cause serious ecological damage to plants and animals on the coast as well as other inhabitants of the ocean. Microbes such as ''Alcanivorax'' provide a major route for the breakdown of these pollutants, and demonstrate how marine bacteria keep the environment in check. Of all the ''Alcanivorax'' species and other oil-degrading microbes, ''Alcanivorax borkumensis'' is one of the most important worldwide due to the fact it produces a wide variety of very efficient oil-degrading enzymes. With this knowledge, ''A. borkumensis'' could provide a useful tool for bioremediation of oil spills.<br />
<br />
==Genome Structure==<br />
[[Image:Lorenzo.gif|thumbnail|200px|Figure 3. Mechanisms for oil degradation and survival encoded by the ''A. borkumensis SK2'' genome. Image from Victor de Lorenzo[http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html]]]<br />
<br />
The ''Alcanivorax borkumensis'' strain SK2, isolated from a seawater sediment sample in the North Sea at a site located near the Isle of Borkum, was the first hydrocarbonoclastic bacterium to be sequenced and was completed by Susanne Schneiker et al. It's genome consists of a single circular chromosome with 3,120,143 base pairs and an average G+C content of 54.7%. The genomic analysis of ''A. borkumensis SK2'' revealed several new insights into the bacterium's role for (i) n-alkane degradation (which includes metabolism, biosurfactant production and biofilm production), (ii) it's system for capturing or scavenging the small amounts of nitrogen, phosphorous, sulfur, and other elements in a nutrient-poor marine environment which allows for more efficient alkane degradation due to their main limitation of nutrient availability, (iii) as well as means for coping with stress factors such as high salt contents and high UV radiation since it thrives mostly in the upper layers in the ocean where UV light is encountered (Figure 3).<br />
<br />
It's genome encodes several systems for the catabolism of hydrocarbons which allow the bacertium to degrade all sorts of alkanes such as AlkB1 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C5-C12, and AlkB2 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C8 to C16. Both these systems are located close to the origin of replication of the chromosome. ''A. borkumensis'' is also able to degrade alkanes up to C32, branched aliphatic hydrocarbons, isoprenoid hydrocarbons such as phytane, as well as alkylarenes and alkylcycloalkanes. Thus, the genome encodes for a broad spectrum of systems for the catabolism of hydrocarbons, giving it a competitive advantage over other oil-degrading marine microbial communities. To deal with the damaging effects of UV light, ''A. borkumensis'' has a number of genes that reduce the damage. These include the full genes for DNA alkylation, recombinational and nucleotide excision repair, base excision repair, as well as the SOS response [4].<br />
<br />
==Cell Structure, Metabolism and Life Cycle==<br />
''Alcanivorax borkumensis'', a Gram-negative, rod-shaped chemoorganotroph, is able to use n-alkanes as its principle carbon and energy source by use of the broad spectrum of oil-degrading enzymes it possesses, but they can also use a limited number of organic compounds such as aliphatic hydrocarbons, volatile fatty acids, and pyruvate. However, it cannot utilize carbon sources such as sugars or amino acids. Cells grown with pyruvate were observed to be 2.0-3.0 micrometers in length and 0.4-07 micrometers in diameter, however, cells were shorter (1.0-1.5 micrometers in length) when cells were grown with n-alkanes as the carbon source [5]. When the slow growing ''A. borkumensis'' uses n-alkanes exclusively, the microbes produce extracellular and membrane-bound surface-active glucose lipids called biosurfactants. These biosurfactants reduce the surface tension of water from 72 to 29 mN m-1 and act as natural emulsifiers which enhances the break up of oil-in-water emulsions and prevent degradation of alkanes [4,5]. Due to the low solubility of oil in water, most oil degradation takes place at the oil-water interface where ''A. borkumensis'' attaches and forms a biofilm around the oil droplets as depicted in Figure 3.<br />
<br />
==Ecology==<br />
''Alcanivorix'' is a novel species living in the oceans that plays a major role in keeping our pristine oceans in good condition as well as the inhabitants of the ocean and the inhabitants of the coastal regions. It has been detected worldwide in places such as the Mediterranean Sea, Pacific Ocean, and the Arctic Sea. In seawater with high concentrations of n-alkanes (as a result of oil spills, natural oil fields, and/or processing plants), ''Alkanivorax'' quickly becomes the predominant microbial community and is found in higher populations when compared to ''Alkanivorax'' in unpolluted seawater. There have been several recent fields studies on bacterial on community dynamics and hydrocarbon degradation in coastal areas contaminated with oil. These field studies have demonstrated the immense importance of ''Alcanivorax'' (particularly ''A. Borkumensis'') in oil-spill bioremediation [2].<br />
<br />
==References==<br />
[1] [http://ijs.sgmjournals.org/cgi/content/abstract/53/1/331 Fernandez-Martinez, Javier, Maria J. Pujalte, Jesus Garcia-Martinez, Manuel Mata, Esperanza Garay, and Francisco Rodriguez-Valera. "Description of ''Alcanivorax Venustensis'' sp. nov. and Reclassification of ''Fundibacter Jadensis'' DSM 12178T (Bruns and Berthe-Corti 1999) As ''Alcanivorax Jadensis'' comb. nov., Members of the Emended Genus ''Alcanivorax''." International Journal of Systematic and Evolutionary Microbiology 53 (2003): 331-338.]<br />
<br />
[2] [http://www.blackwell-synergy.com/doi/pdf/10.1046/j.1468-2920.2003.00468.x Hara, Akihiro, Kazuaki Syutsubo, and Shigeaki Harayama. "''Alcanivorax'' Which Prevails In Oil-contaminated Seawater Exhibits Broad Substrate Specificity For Alkane Degradation." Environmental Microbiology 5.9 (2003): 746-753.]<br />
<br />
[3] [http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html Lorenzo, Víctor De. "Blueprint of an Oil-eating Bacterium." Nature Biotechnology 24 (2006): 952-953.]<br />
<br />
[4] [http://www.ncbi.nlm.nih.gov/pubmed/16878126 Schneiker, S. et al. "Genome Sequence of the Ubiquitous Hydrocarbon-degrading Marine Bacterium A''lcanivorax Borkumensis''." Nature Biotechnology 24 (2006): 997-1004.]<br />
<br />
[5] [http://ijs.sgmjournals.org/cgi/content/abstract/48/2/339 Yakimov, Michail M., Peter N. Golyshin, Siegmund Lang, Edward R. B. Moore, Wolf-Rainer Abraham, Heinrich Lunsdorf, and Kenneth N. Timmis. "''Alcanivorax Borkumensis'' gen. nov., sp. nov., A New, Hydrocarbon-degrading And Surfactant-producing Marine Bacterium." International Journal of Systematic Bacteriology 48 (1998): 339-348.]<br />
<br />
==Author==<br />
Page authored by Andrew Buss, student of [http://www.kbs.msu.edu/faculty/lennon/ Prof. Jay Lennon] at Michigan State University.</div>Bussand1https://microbewiki.kenyon.edu/index.php?title=Alcanivorax&diff=29768Alcanivorax2008-04-07T00:44:35Z<p>Bussand1: /* Cell Structure, Metabolism and Life Cycle */</p>
<hr />
<div>[[Image:AlcVorax PROCARYOTES01b-1.JPG|thumbnail|300px|Figure 1. ''Alcanivorax borkumensis''. Image from Helmholtz Centre for Infection Research[http://www.helmholtz-hzi.de/en/news_public_relation/press_releases/view/article/complete/oil_tanker_accidents_as_a_source_of_food/]]]<br />
<br />
==Classification==<br />
<br />
'''Bacteria'''; Phylum: '''Proteobacteria'''; Class: '''Gammaproteobacteria'''; Order: '''Oceanospirillales'''; Family: '''Alcanivoracaceae'''<br />
<br />
===Species===<br />
<br />
{|<br />
| height="10" bgcolor="#FFDF95" |<br />
'''NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Tree&id=2&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy]'''<br />
|}<br />
<br />
*''Alcanivorax balearicum''<br />
*''Alcanivorax borkumensis''<br />
*''Alcanivorax dieselolei''<br />
*''Alcanivorax indicus''<br />
*''Alcanivorax jadensis''<br />
*''Alcanivorax venustensis''<br />
<br />
==Description and Significance==<br />
[[Image:PWS tanker oil spill.jpg|thumbnail|200px|Figure 2. Supertanker Exxon Valdez grounded on Bligh Reef which released 11 million gallons of crude oil into the water. This oil-contaminated seawater is the preferred habitat for ''Alcanivorax''. Image from USGS[http://menlocampus.wr.usgs.gov/50years/accomplishments/oil.html]]]<br />
<br />
''Alcanivorax'', first described in 1998, is a Gram-negative, halophilic, aerobic, rod-shaped, oil-degrading marine bacterium that is found in low abundances in unpolluted environments in the upper layers of the ocean, but quickly becomes the predominant microbe in oil-contaminated open oceans and coastal waters when nitrogen and phosphorus are not limiting [2]. When conditions in these moderately halophilic environments are right, ''Alcanivorax'' may make up 80-90% of the oil-degrading microbes present in the area [4]. It is described as a non-motile bactertium which is true for species such as ''Alcanivorax borkumensis'', but other species such as ''Alcanivorax venustensis'' were described to be motile by polar flagella [1]. The optimial conditions described for A.borkumensis growth include temperatures in the range of 20-30 degrees celsius, and a NaCl concentration of 3-10%.<br />
<br />
As a result of their profound ability to degrade and live predominately on alkanes, as well as to become the dominant microbes in oil-contaminated areas, ''Alcanivorax'' plays a huge role in the biological cleanup of oil-contaminated environments. These oil-contaminated environments in the ocean are largely due to anthropogenic sources such as oil spills caused by tankers accidents (Figure 2), and cause serious ecological damage to plants and animals on the coast as well as other inhabitants of the ocean. Microbes such as ''Alcanivorax'' provide a major route for the breakdown of these pollutants, and demonstrate how marine bacteria keep the environment in check. Of all the ''Alcanivorax'' species and other oil-degrading microbes, ''Alcanivorax borkumensis'' is one of the most important worldwide due to the fact it produces a wide variety of very efficient oil-degrading enzymes. With this knowledge, ''A. borkumensis'' could provide a useful tool for bioremediation of oil spills.<br />
<br />
==Genome Structure==<br />
[[Image:Lorenzo.gif|thumbnail|200px|Figure 3. Mechanisms for oil degradation and survival encoded by the ''A. borkumensis SK2'' genome. Image from Victor de Lorenzo[http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html]]]<br />
<br />
The ''Alcanivorax borkumensis'' strain SK2, isolated from a seawater sediment sample in the North Sea at a site located near the Isle of Borkum, was the first hydrocarbonoclastic bacterium to be sequenced and was completed by Susanne Schneiker et al. It's genome consists of a single circular chromosome with 3,120,143 base pairs and an average G+C content of 54.7%. The genomic analysis of ''A. borkumensis SK2'' revealed several new insights into the bacterium's role for (i) n-alkane degradation (which includes metabolism, biosurfactant production and biofilm production), (ii) it's system for capturing or scavenging the small amounts of nitrogen, phosphorous, sulfur, and other elements in a nutrient-poor marine environment which allows for more efficient alkane degradation due to their main limitation of nutrient availability, (iii) as well as means for coping with stress factors such as high salt contents and high UV radiation since it thrives mostly in the upper layers in the ocean where UV light is encountered (Figure 3).<br />
<br />
It's genome encodes several systems for the catabolism of hydrocarbons which allow the bacertium to degrade all sorts of alkanes such as AlkB1 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C5-C12, and AlkB2 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C8 to C16. Both these systems are located close to the origin of replication of the chromosome. ''A. borkumensis'' is also able to degrade alkanes up to C32, branched aliphatic hydrocarbons, isoprenoid hydrocarbons such as phytane, as well as alkylarenes and alkylcycloalkanes. Thus, the genome encodes for a broad spectrum of systems for the catabolism of hydrocarbons, giving it a competitive advantage over other oil-degrading marine microbial communities. To deal with the damaging effects of UV light, ''A. borkumensis'' has a number of genes that reduce the damage. These include the full genes for DNA alkylation, recombinational and nucleotide excision repair, base excision repair, as well as the SOS response [4].<br />
<br />
==Cell Structure, Metabolism and Life Cycle==<br />
''Alcanivorax borkumensis'', a Gram-negative, rod-shaped chemoorganotroph, is able to use n-alkanes as its principle carbon and energy source by use of the broad spectrum of oil-degrading enzymes it possesses, but they can also use a limited number of organic compounds such as aliphatic hydrocarbons, volatile fatty acids, and pyruvate. However, it cannot utilize carbon sources such as sugars or amino acids. Cells grown with pyruvate were observed to be 2.0-3.0 micrometers in length and 0.4-07 micrometers in diameter, however, cells were shorter (1.0-1.5 micrometers in length) when cells were grown with n-alkanes as the carbon source [5]. When the slow growing ''A. borkumensis'' uses n-alkanes exclusively, the microbes produce extracellular and membrane-bound surface-active glucose lipids called biosurfactants. These biosurfactants reduce the surface tension of water from 72 to 29 mN m-1 and act as natural emulsifiers which enhances the break up of oil-in-water emulsions and prevent degradation of alkanes [4,5]. Due to the low solubility of oil in water, most oil degradation takes place at the oil-water interface where ''A. borkumensis'' attaches and forms a biofilm around the oil droplets as depicted in Figure 3.<br />
<br />
==Ecology and Pathogenesis==<br />
Habitat; symbiosis; biogeochemical significance; contributions to environment.<br />
<br />
==References==<br />
[1] [http://ijs.sgmjournals.org/cgi/content/abstract/53/1/331 Fernandez-Martinez, Javier, Maria J. Pujalte, Jesus Garcia-Martinez, Manuel Mata, Esperanza Garay, and Francisco Rodriguez-Valera. "Description of ''Alcanivorax Venustensis'' sp. nov. and Reclassification of ''Fundibacter Jadensis'' DSM 12178T (Bruns and Berthe-Corti 1999) As ''Alcanivorax Jadensis'' comb. nov., Members of the Emended Genus ''Alcanivorax''." International Journal of Systematic and Evolutionary Microbiology 53 (2003): 331-338.]<br />
<br />
[2] [http://www.blackwell-synergy.com/doi/pdf/10.1046/j.1468-2920.2003.00468.x Hara, Akihiro, Kazuaki Syutsubo, and Shigeaki Harayama. "''Alcanivorax'' Which Prevails In Oil-contaminated Seawater Exhibits Broad Substrate Specificity For Alkane Degradation." Environmental Microbiology 5.9 (2003): 746-753.]<br />
<br />
[3] [http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html Lorenzo, Víctor De. "Blueprint of an Oil-eating Bacterium." Nature Biotechnology 24 (2006): 952-953.]<br />
<br />
[4] [http://www.ncbi.nlm.nih.gov/pubmed/16878126 Schneiker, S. et al. "Genome Sequence of the Ubiquitous Hydrocarbon-degrading Marine Bacterium A''lcanivorax Borkumensis''." Nature Biotechnology 24 (2006): 997-1004.]<br />
<br />
[5] [http://ijs.sgmjournals.org/cgi/content/abstract/48/2/339 Yakimov, Michail M., Peter N. Golyshin, Siegmund Lang, Edward R. B. Moore, Wolf-Rainer Abraham, Heinrich Lunsdorf, and Kenneth N. Timmis. "''Alcanivorax Borkumensis'' gen. nov., sp. nov., A New, Hydrocarbon-degrading And Surfactant-producing Marine Bacterium." International Journal of Systematic Bacteriology 48 (1998): 339-348.]<br />
<br />
==Author==<br />
Page authored by Andrew Buss, student of [http://www.kbs.msu.edu/faculty/lennon/ Prof. Jay Lennon] at Michigan State University.</div>Bussand1https://microbewiki.kenyon.edu/index.php?title=Alcanivorax&diff=29764Alcanivorax2008-04-06T22:43:16Z<p>Bussand1: /* Genome Structure */</p>
<hr />
<div>[[Image:AlcVorax PROCARYOTES01b-1.JPG|thumbnail|300px|Figure 1. ''Alcanivorax borkumensis''. Image from Helmholtz Centre for Infection Research[http://www.helmholtz-hzi.de/en/news_public_relation/press_releases/view/article/complete/oil_tanker_accidents_as_a_source_of_food/]]]<br />
<br />
==Classification==<br />
<br />
'''Bacteria'''; Phylum: '''Proteobacteria'''; Class: '''Gammaproteobacteria'''; Order: '''Oceanospirillales'''; Family: '''Alcanivoracaceae'''<br />
<br />
===Species===<br />
<br />
{|<br />
| height="10" bgcolor="#FFDF95" |<br />
'''NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Tree&id=2&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy]'''<br />
|}<br />
<br />
*''Alcanivorax balearicum''<br />
*''Alcanivorax borkumensis''<br />
*''Alcanivorax dieselolei''<br />
*''Alcanivorax indicus''<br />
*''Alcanivorax jadensis''<br />
*''Alcanivorax venustensis''<br />
<br />
==Description and Significance==<br />
[[Image:PWS tanker oil spill.jpg|thumbnail|200px|Figure 2. Supertanker Exxon Valdez grounded on Bligh Reef which released 11 million gallons of crude oil into the water. This oil-contaminated seawater is the preferred habitat for ''Alcanivorax''. Image from USGS[http://menlocampus.wr.usgs.gov/50years/accomplishments/oil.html]]]<br />
<br />
''Alcanivorax'', first described in 1998, is a Gram-negative, halophilic, aerobic, rod-shaped, oil-degrading marine bacterium that is found in low abundances in unpolluted environments in the upper layers of the ocean, but quickly becomes the predominant microbe in oil-contaminated open oceans and coastal waters when nitrogen and phosphorus are not limiting [2]. When conditions in these moderately halophilic environments are right, ''Alcanivorax'' may make up 80-90% of the oil-degrading microbes present in the area [4]. It is described as a non-motile bactertium which is true for species such as ''Alcanivorax borkumensis'', but other species such as ''Alcanivorax venustensis'' were described to be motile by polar flagella [1]. The optimial conditions described for A.borkumensis growth include temperatures in the range of 20-30 degrees celsius, and a NaCl concentration of 3-10%.<br />
<br />
As a result of their profound ability to degrade and live predominately on alkanes, as well as to become the dominant microbes in oil-contaminated areas, ''Alcanivorax'' plays a huge role in the biological cleanup of oil-contaminated environments. These oil-contaminated environments in the ocean are largely due to anthropogenic sources such as oil spills caused by tankers accidents (Figure 2), and cause serious ecological damage to plants and animals on the coast as well as other inhabitants of the ocean. Microbes such as ''Alcanivorax'' provide a major route for the breakdown of these pollutants, and demonstrate how marine bacteria keep the environment in check. Of all the ''Alcanivorax'' species and other oil-degrading microbes, ''Alcanivorax borkumensis'' is one of the most important worldwide due to the fact it produces a wide variety of very efficient oil-degrading enzymes. With this knowledge, ''A. borkumensis'' could provide a useful tool for bioremediation of oil spills.<br />
<br />
==Genome Structure==<br />
[[Image:Lorenzo.gif|thumbnail|200px|Figure 3. Mechanisms for oil degradation and survival encoded by the ''A. borkumensis SK2'' genome. Image from Victor de Lorenzo[http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html]]]<br />
<br />
The ''Alcanivorax borkumensis'' strain SK2, isolated from a seawater sediment sample in the North Sea at a site located near the Isle of Borkum, was the first hydrocarbonoclastic bacterium to be sequenced and was completed by Susanne Schneiker et al. It's genome consists of a single circular chromosome with 3,120,143 base pairs and an average G+C content of 54.7%. The genomic analysis of ''A. borkumensis SK2'' revealed several new insights into the bacterium's role for (i) n-alkane degradation (which includes metabolism, biosurfactant production and biofilm production), (ii) it's system for capturing or scavenging the small amounts of nitrogen, phosphorous, sulfur, and other elements in a nutrient-poor marine environment which allows for more efficient alkane degradation due to their main limitation of nutrient availability, (iii) as well as means for coping with stress factors such as high salt contents and high UV radiation since it thrives mostly in the upper layers in the ocean where UV light is encountered (Figure 3).<br />
<br />
It's genome encodes several systems for the catabolism of hydrocarbons which allow the bacertium to degrade all sorts of alkanes such as AlkB1 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C5-C12, and AlkB2 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C8 to C16. Both these systems are located close to the origin of replication of the chromosome. ''A. borkumensis'' is also able to degrade alkanes up to C32, branched aliphatic hydrocarbons, isoprenoid hydrocarbons such as phytane, as well as alkylarenes and alkylcycloalkanes. Thus, the genome encodes for a broad spectrum of systems for the catabolism of hydrocarbons, giving it a competitive advantage over other oil-degrading marine microbial communities. To deal with the damaging effects of UV light, ''A. borkumensis'' has a number of genes that reduce the damage. These include the full genes for DNA alkylation, recombinational and nucleotide excision repair, base excision repair, as well as the SOS response [4].<br />
<br />
==Cell Structure, Metabolism and Life Cycle==<br />
''Alcanivorax borkumensis'', a Gram-negative, rod-shaped chemoorganotroph, is able to use n-alkanes as its principle carbon and energy source by use of the broad spectrum of oil-degrading enzymes it possesses, but they can also use a limited number of organic compounds such as aliphatic hydrocarbons, volatile fatty acids, and pyruvate. However, it cannot utilize carbon sources such as sugars or amino acids. Cells grown with pyruvate were observed to be 2.0-3.0 micrometers in length and 0.4-07 micrometers in diameter, however, cells were shorter (1.0-1.5 micrometers in length) when cells were grown with n-alkanes as the carbon source [5]. When the slow growing ''A. borkumensis'' uses n-alkanes exclusively, the microbes produce extracellular and membrane-bound surface-active glucose lipids called biosurfactants. These biosurfactants reduce the surface tension of water from 72 to 29 mN m-1 and act as natural emulsifiers which enhances the break up of oil-in-water emulsions which prevent degradation of alkanes [4,5]. Due to the low solubility of oil in water, most oil degradation takes place at the oil-water interface where ''A. borkumensis'' attaches and forms a biofilm around the oil droplets as depicted in Figure 3.<br />
<br />
==Ecology and Pathogenesis==<br />
Habitat; symbiosis; biogeochemical significance; contributions to environment.<br />
<br />
==References==<br />
[1] [http://ijs.sgmjournals.org/cgi/content/abstract/53/1/331 Fernandez-Martinez, Javier, Maria J. Pujalte, Jesus Garcia-Martinez, Manuel Mata, Esperanza Garay, and Francisco Rodriguez-Valera. "Description of ''Alcanivorax Venustensis'' sp. nov. and Reclassification of ''Fundibacter Jadensis'' DSM 12178T (Bruns and Berthe-Corti 1999) As ''Alcanivorax Jadensis'' comb. nov., Members of the Emended Genus ''Alcanivorax''." International Journal of Systematic and Evolutionary Microbiology 53 (2003): 331-338.]<br />
<br />
[2] [http://www.blackwell-synergy.com/doi/pdf/10.1046/j.1468-2920.2003.00468.x Hara, Akihiro, Kazuaki Syutsubo, and Shigeaki Harayama. "''Alcanivorax'' Which Prevails In Oil-contaminated Seawater Exhibits Broad Substrate Specificity For Alkane Degradation." Environmental Microbiology 5.9 (2003): 746-753.]<br />
<br />
[3] [http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html Lorenzo, Víctor De. "Blueprint of an Oil-eating Bacterium." Nature Biotechnology 24 (2006): 952-953.]<br />
<br />
[4] [http://www.ncbi.nlm.nih.gov/pubmed/16878126 Schneiker, S. et al. "Genome Sequence of the Ubiquitous Hydrocarbon-degrading Marine Bacterium A''lcanivorax Borkumensis''." Nature Biotechnology 24 (2006): 997-1004.]<br />
<br />
[5] [http://ijs.sgmjournals.org/cgi/content/abstract/48/2/339 Yakimov, Michail M., Peter N. Golyshin, Siegmund Lang, Edward R. B. Moore, Wolf-Rainer Abraham, Heinrich Lunsdorf, and Kenneth N. Timmis. "''Alcanivorax Borkumensis'' gen. nov., sp. nov., A New, Hydrocarbon-degrading And Surfactant-producing Marine Bacterium." International Journal of Systematic Bacteriology 48 (1998): 339-348.]<br />
<br />
==Author==<br />
Page authored by Andrew Buss, student of [http://www.kbs.msu.edu/faculty/lennon/ Prof. Jay Lennon] at Michigan State University.</div>Bussand1https://microbewiki.kenyon.edu/index.php?title=Alcanivorax&diff=29763Alcanivorax2008-04-06T22:42:07Z<p>Bussand1: /* Cell Structure, Metabolism and Life Cycle */</p>
<hr />
<div>[[Image:AlcVorax PROCARYOTES01b-1.JPG|thumbnail|300px|Figure 1. ''Alcanivorax borkumensis''. Image from Helmholtz Centre for Infection Research[http://www.helmholtz-hzi.de/en/news_public_relation/press_releases/view/article/complete/oil_tanker_accidents_as_a_source_of_food/]]]<br />
<br />
==Classification==<br />
<br />
'''Bacteria'''; Phylum: '''Proteobacteria'''; Class: '''Gammaproteobacteria'''; Order: '''Oceanospirillales'''; Family: '''Alcanivoracaceae'''<br />
<br />
===Species===<br />
<br />
{|<br />
| height="10" bgcolor="#FFDF95" |<br />
'''NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Tree&id=2&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy]'''<br />
|}<br />
<br />
*''Alcanivorax balearicum''<br />
*''Alcanivorax borkumensis''<br />
*''Alcanivorax dieselolei''<br />
*''Alcanivorax indicus''<br />
*''Alcanivorax jadensis''<br />
*''Alcanivorax venustensis''<br />
<br />
==Description and Significance==<br />
[[Image:PWS tanker oil spill.jpg|thumbnail|200px|Figure 2. Supertanker Exxon Valdez grounded on Bligh Reef which released 11 million gallons of crude oil into the water. This oil-contaminated seawater is the preferred habitat for ''Alcanivorax''. Image from USGS[http://menlocampus.wr.usgs.gov/50years/accomplishments/oil.html]]]<br />
<br />
''Alcanivorax'', first described in 1998, is a Gram-negative, halophilic, aerobic, rod-shaped, oil-degrading marine bacterium that is found in low abundances in unpolluted environments in the upper layers of the ocean, but quickly becomes the predominant microbe in oil-contaminated open oceans and coastal waters when nitrogen and phosphorus are not limiting [2]. When conditions in these moderately halophilic environments are right, ''Alcanivorax'' may make up 80-90% of the oil-degrading microbes present in the area [4]. It is described as a non-motile bactertium which is true for species such as ''Alcanivorax borkumensis'', but other species such as ''Alcanivorax venustensis'' were described to be motile by polar flagella [1]. The optimial conditions described for A.borkumensis growth include temperatures in the range of 20-30 degrees celsius, and a NaCl concentration of 3-10%.<br />
<br />
As a result of their profound ability to degrade and live predominately on alkanes, as well as to become the dominant microbes in oil-contaminated areas, ''Alcanivorax'' plays a huge role in the biological cleanup of oil-contaminated environments. These oil-contaminated environments in the ocean are largely due to anthropogenic sources such as oil spills caused by tankers accidents (Figure 2), and cause serious ecological damage to plants and animals on the coast as well as other inhabitants of the ocean. Microbes such as ''Alcanivorax'' provide a major route for the breakdown of these pollutants, and demonstrate how marine bacteria keep the environment in check. Of all the ''Alcanivorax'' species and other oil-degrading microbes, ''Alcanivorax borkumensis'' is one of the most important worldwide due to the fact it produces a wide variety of very efficient oil-degrading enzymes. With this knowledge, ''A. borkumensis'' could provide a useful tool for bioremediation of oil spills.<br />
<br />
==Genome Structure==<br />
[[Image:Lorenzo.gif|thumbnail|200px|Figure 3. Mechanisms for oil degradation and survival encoded by the ''A. borkumensis SK2'' genome. Image from Victor de Lorenzo[http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html]]]<br />
<br />
The ''Alcanivorax borkumensis'' strain SK2, isolated from a seawater sediment sample in the North Sea at a site located near the Isle of Borkum, was the first hydrocarbonoclastic bacterium to be sequenced and was completed by Susanne Schneiker et al. It's genome consists of a single circular chromosome with 3,120,143 base pairs with an average G+C content of 54.7%. The genomic analysis of ''A. borkumensis SK2'' revealed several new insights into the bacterium's role for (i) n-alkane degradation (which includes metabolism, biosurfactant production and biofilm production), (ii) it's system for capturing or scavenging the small amounts of nitrogen, phosphorous, sulfur, and other elements in a nutrient-poor marine environment which allows for more efficient alkane degradation due to their main limitation of nutrient availability, (iii) as well as means for coping with stress factors such as high salt contents and high UV radiation since it thrives mostly in the upper layers in the ocean where UV light is encountered (Figure 3).<br />
<br />
It's genome encodes several systems for the catabolism of hydrocarbons which allow the bacertium to degrade all sorts of alkanes such as AlkB1 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C5-C12, and AlkB2 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C8 to C16. Both these systems are located close to the origin of replication of the chromosome. ''A. borkumensis'' is also able to degrade alkanes up to C32, branched aliphatic hydrocarbons, isoprenoid hydrocarbons such as phytane, as well as alkylarenes and alkylcycloalkanes. Thus, the genome encodes for a broad spectrum of systems for the catabolism of hydrocarbons, giving it a competitive advantage over other oil-degrading marine microbial communities. To deal with the damaging effects of UV light, ''A. borkumensis'' has a number of genes that reduce the damage. These include the full genes for DNA alkylation, recombinational and nucleotide excision repair, base excision repair, as well as the SOS response [4].<br />
<br />
==Cell Structure, Metabolism and Life Cycle==<br />
''Alcanivorax borkumensis'', a Gram-negative, rod-shaped chemoorganotroph, is able to use n-alkanes as its principle carbon and energy source by use of the broad spectrum of oil-degrading enzymes it possesses, but they can also use a limited number of organic compounds such as aliphatic hydrocarbons, volatile fatty acids, and pyruvate. However, it cannot utilize carbon sources such as sugars or amino acids. Cells grown with pyruvate were observed to be 2.0-3.0 micrometers in length and 0.4-07 micrometers in diameter, however, cells were shorter (1.0-1.5 micrometers in length) when cells were grown with n-alkanes as the carbon source [5]. When the slow growing ''A. borkumensis'' uses n-alkanes exclusively, the microbes produce extracellular and membrane-bound surface-active glucose lipids called biosurfactants. These biosurfactants reduce the surface tension of water from 72 to 29 mN m-1 and act as natural emulsifiers which enhances the break up of oil-in-water emulsions which prevent degradation of alkanes [4,5]. Due to the low solubility of oil in water, most oil degradation takes place at the oil-water interface where ''A. borkumensis'' attaches and forms a biofilm around the oil droplets as depicted in Figure 3.<br />
<br />
==Ecology and Pathogenesis==<br />
Habitat; symbiosis; biogeochemical significance; contributions to environment.<br />
<br />
==References==<br />
[1] [http://ijs.sgmjournals.org/cgi/content/abstract/53/1/331 Fernandez-Martinez, Javier, Maria J. Pujalte, Jesus Garcia-Martinez, Manuel Mata, Esperanza Garay, and Francisco Rodriguez-Valera. "Description of ''Alcanivorax Venustensis'' sp. nov. and Reclassification of ''Fundibacter Jadensis'' DSM 12178T (Bruns and Berthe-Corti 1999) As ''Alcanivorax Jadensis'' comb. nov., Members of the Emended Genus ''Alcanivorax''." International Journal of Systematic and Evolutionary Microbiology 53 (2003): 331-338.]<br />
<br />
[2] [http://www.blackwell-synergy.com/doi/pdf/10.1046/j.1468-2920.2003.00468.x Hara, Akihiro, Kazuaki Syutsubo, and Shigeaki Harayama. "''Alcanivorax'' Which Prevails In Oil-contaminated Seawater Exhibits Broad Substrate Specificity For Alkane Degradation." Environmental Microbiology 5.9 (2003): 746-753.]<br />
<br />
[3] [http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html Lorenzo, Víctor De. "Blueprint of an Oil-eating Bacterium." Nature Biotechnology 24 (2006): 952-953.]<br />
<br />
[4] [http://www.ncbi.nlm.nih.gov/pubmed/16878126 Schneiker, S. et al. "Genome Sequence of the Ubiquitous Hydrocarbon-degrading Marine Bacterium A''lcanivorax Borkumensis''." Nature Biotechnology 24 (2006): 997-1004.]<br />
<br />
[5] [http://ijs.sgmjournals.org/cgi/content/abstract/48/2/339 Yakimov, Michail M., Peter N. Golyshin, Siegmund Lang, Edward R. B. Moore, Wolf-Rainer Abraham, Heinrich Lunsdorf, and Kenneth N. Timmis. "''Alcanivorax Borkumensis'' gen. nov., sp. nov., A New, Hydrocarbon-degrading And Surfactant-producing Marine Bacterium." International Journal of Systematic Bacteriology 48 (1998): 339-348.]<br />
<br />
==Author==<br />
Page authored by Andrew Buss, student of [http://www.kbs.msu.edu/faculty/lennon/ Prof. Jay Lennon] at Michigan State University.</div>Bussand1https://microbewiki.kenyon.edu/index.php?title=Alcanivorax&diff=29762Alcanivorax2008-04-06T22:40:42Z<p>Bussand1: /* Cell Structure, Metabolism and Life Cycle */</p>
<hr />
<div>[[Image:AlcVorax PROCARYOTES01b-1.JPG|thumbnail|300px|Figure 1. ''Alcanivorax borkumensis''. Image from Helmholtz Centre for Infection Research[http://www.helmholtz-hzi.de/en/news_public_relation/press_releases/view/article/complete/oil_tanker_accidents_as_a_source_of_food/]]]<br />
<br />
==Classification==<br />
<br />
'''Bacteria'''; Phylum: '''Proteobacteria'''; Class: '''Gammaproteobacteria'''; Order: '''Oceanospirillales'''; Family: '''Alcanivoracaceae'''<br />
<br />
===Species===<br />
<br />
{|<br />
| height="10" bgcolor="#FFDF95" |<br />
'''NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Tree&id=2&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy]'''<br />
|}<br />
<br />
*''Alcanivorax balearicum''<br />
*''Alcanivorax borkumensis''<br />
*''Alcanivorax dieselolei''<br />
*''Alcanivorax indicus''<br />
*''Alcanivorax jadensis''<br />
*''Alcanivorax venustensis''<br />
<br />
==Description and Significance==<br />
[[Image:PWS tanker oil spill.jpg|thumbnail|200px|Figure 2. Supertanker Exxon Valdez grounded on Bligh Reef which released 11 million gallons of crude oil into the water. This oil-contaminated seawater is the preferred habitat for ''Alcanivorax''. Image from USGS[http://menlocampus.wr.usgs.gov/50years/accomplishments/oil.html]]]<br />
<br />
''Alcanivorax'', first described in 1998, is a Gram-negative, halophilic, aerobic, rod-shaped, oil-degrading marine bacterium that is found in low abundances in unpolluted environments in the upper layers of the ocean, but quickly becomes the predominant microbe in oil-contaminated open oceans and coastal waters when nitrogen and phosphorus are not limiting [2]. When conditions in these moderately halophilic environments are right, ''Alcanivorax'' may make up 80-90% of the oil-degrading microbes present in the area [4]. It is described as a non-motile bactertium which is true for species such as ''Alcanivorax borkumensis'', but other species such as ''Alcanivorax venustensis'' were described to be motile by polar flagella [1]. The optimial conditions described for A.borkumensis growth include temperatures in the range of 20-30 degrees celsius, and a NaCl concentration of 3-10%.<br />
<br />
As a result of their profound ability to degrade and live predominately on alkanes, as well as to become the dominant microbes in oil-contaminated areas, ''Alcanivorax'' plays a huge role in the biological cleanup of oil-contaminated environments. These oil-contaminated environments in the ocean are largely due to anthropogenic sources such as oil spills caused by tankers accidents (Figure 2), and cause serious ecological damage to plants and animals on the coast as well as other inhabitants of the ocean. Microbes such as ''Alcanivorax'' provide a major route for the breakdown of these pollutants, and demonstrate how marine bacteria keep the environment in check. Of all the ''Alcanivorax'' species and other oil-degrading microbes, ''Alcanivorax borkumensis'' is one of the most important worldwide due to the fact it produces a wide variety of very efficient oil-degrading enzymes. With this knowledge, ''A. borkumensis'' could provide a useful tool for bioremediation of oil spills.<br />
<br />
==Genome Structure==<br />
[[Image:Lorenzo.gif|thumbnail|200px|Figure 3. Mechanisms for oil degradation and survival encoded by the ''A. borkumensis SK2'' genome. Image from Victor de Lorenzo[http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html]]]<br />
<br />
The ''Alcanivorax borkumensis'' strain SK2, isolated from a seawater sediment sample in the North Sea at a site located near the Isle of Borkum, was the first hydrocarbonoclastic bacterium to be sequenced and was completed by Susanne Schneiker et al. It's genome consists of a single circular chromosome with 3,120,143 base pairs with an average G+C content of 54.7%. The genomic analysis of ''A. borkumensis SK2'' revealed several new insights into the bacterium's role for (i) n-alkane degradation (which includes metabolism, biosurfactant production and biofilm production), (ii) it's system for capturing or scavenging the small amounts of nitrogen, phosphorous, sulfur, and other elements in a nutrient-poor marine environment which allows for more efficient alkane degradation due to their main limitation of nutrient availability, (iii) as well as means for coping with stress factors such as high salt contents and high UV radiation since it thrives mostly in the upper layers in the ocean where UV light is encountered (Figure 3).<br />
<br />
It's genome encodes several systems for the catabolism of hydrocarbons which allow the bacertium to degrade all sorts of alkanes such as AlkB1 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C5-C12, and AlkB2 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C8 to C16. Both these systems are located close to the origin of replication of the chromosome. ''A. borkumensis'' is also able to degrade alkanes up to C32, branched aliphatic hydrocarbons, isoprenoid hydrocarbons such as phytane, as well as alkylarenes and alkylcycloalkanes. Thus, the genome encodes for a broad spectrum of systems for the catabolism of hydrocarbons, giving it a competitive advantage over other oil-degrading marine microbial communities. To deal with the damaging effects of UV light, ''A. borkumensis'' has a number of genes that reduce the damage. These include the full genes for DNA alkylation, recombinational and nucleotide excision repair, base excision repair, as well as the SOS response [4].<br />
<br />
==Cell Structure, Metabolism and Life Cycle==<br />
''Alcanivorax borkumensis'', a Gram-negative, rod-shaped chemoorganotroph, is able to use n-alkanes as its principle carbon and energy source by use of the broad spectrum of oil-degrading enzymes it possesses, but they can also use a limited number of organic compounds such as aliphatic hydrocarbons, volatile fatty acids, and pyruvate. However, it cannot utilize carbon sources such as sugars or amino acids. Cells grown with pyruvate were observed to be 2.0-3.0 micrometers in length and 0.4-07 micrometers in diameter, however, cells were shorter (1.0-1.5 micrometers in length) when cells were grown with n-alkanes [5]. When the slow growing ''A. borkumensis'' uses n-alkanes exclusively, the microbes produce extracellular and membrane-bound surface-active glucose lipids called biosurfactants. These biosurfactants reduce the surface tension of water from 72 to 29 mN m-1 and act as natural emulsifiers which enhances the break up of oil-in-water emulsions which prevent degradation of alkanes [4,5]. Due to the low solubility of oil in water, most oil degradation takes place at the oil-water interface where ''A. borkumensis'' attaches and forms a biofilm around the oil droplets as depicted in Figure 3.<br />
<br />
==Ecology and Pathogenesis==<br />
Habitat; symbiosis; biogeochemical significance; contributions to environment.<br />
<br />
==References==<br />
[1] [http://ijs.sgmjournals.org/cgi/content/abstract/53/1/331 Fernandez-Martinez, Javier, Maria J. Pujalte, Jesus Garcia-Martinez, Manuel Mata, Esperanza Garay, and Francisco Rodriguez-Valera. "Description of ''Alcanivorax Venustensis'' sp. nov. and Reclassification of ''Fundibacter Jadensis'' DSM 12178T (Bruns and Berthe-Corti 1999) As ''Alcanivorax Jadensis'' comb. nov., Members of the Emended Genus ''Alcanivorax''." International Journal of Systematic and Evolutionary Microbiology 53 (2003): 331-338.]<br />
<br />
[2] [http://www.blackwell-synergy.com/doi/pdf/10.1046/j.1468-2920.2003.00468.x Hara, Akihiro, Kazuaki Syutsubo, and Shigeaki Harayama. "''Alcanivorax'' Which Prevails In Oil-contaminated Seawater Exhibits Broad Substrate Specificity For Alkane Degradation." Environmental Microbiology 5.9 (2003): 746-753.]<br />
<br />
[3] [http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html Lorenzo, Víctor De. "Blueprint of an Oil-eating Bacterium." Nature Biotechnology 24 (2006): 952-953.]<br />
<br />
[4] [http://www.ncbi.nlm.nih.gov/pubmed/16878126 Schneiker, S. et al. "Genome Sequence of the Ubiquitous Hydrocarbon-degrading Marine Bacterium A''lcanivorax Borkumensis''." Nature Biotechnology 24 (2006): 997-1004.]<br />
<br />
[5] [http://ijs.sgmjournals.org/cgi/content/abstract/48/2/339 Yakimov, Michail M., Peter N. Golyshin, Siegmund Lang, Edward R. B. Moore, Wolf-Rainer Abraham, Heinrich Lunsdorf, and Kenneth N. Timmis. "''Alcanivorax Borkumensis'' gen. nov., sp. nov., A New, Hydrocarbon-degrading And Surfactant-producing Marine Bacterium." International Journal of Systematic Bacteriology 48 (1998): 339-348.]<br />
<br />
==Author==<br />
Page authored by Andrew Buss, student of [http://www.kbs.msu.edu/faculty/lennon/ Prof. Jay Lennon] at Michigan State University.</div>Bussand1https://microbewiki.kenyon.edu/index.php?title=Alcanivorax&diff=29761Alcanivorax2008-04-06T21:06:25Z<p>Bussand1: /* Ecology and Pathogenesis */</p>
<hr />
<div>[[Image:AlcVorax PROCARYOTES01b-1.JPG|thumbnail|300px|Figure 1. ''Alcanivorax borkumensis''. Image from Helmholtz Centre for Infection Research[http://www.helmholtz-hzi.de/en/news_public_relation/press_releases/view/article/complete/oil_tanker_accidents_as_a_source_of_food/]]]<br />
<br />
==Classification==<br />
<br />
'''Bacteria'''; Phylum: '''Proteobacteria'''; Class: '''Gammaproteobacteria'''; Order: '''Oceanospirillales'''; Family: '''Alcanivoracaceae'''<br />
<br />
===Species===<br />
<br />
{|<br />
| height="10" bgcolor="#FFDF95" |<br />
'''NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Tree&id=2&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy]'''<br />
|}<br />
<br />
*''Alcanivorax balearicum''<br />
*''Alcanivorax borkumensis''<br />
*''Alcanivorax dieselolei''<br />
*''Alcanivorax indicus''<br />
*''Alcanivorax jadensis''<br />
*''Alcanivorax venustensis''<br />
<br />
==Description and Significance==<br />
[[Image:PWS tanker oil spill.jpg|thumbnail|200px|Figure 2. Supertanker Exxon Valdez grounded on Bligh Reef which released 11 million gallons of crude oil into the water. This oil-contaminated seawater is the preferred habitat for ''Alcanivorax''. Image from USGS[http://menlocampus.wr.usgs.gov/50years/accomplishments/oil.html]]]<br />
<br />
''Alcanivorax'', first described in 1998, is a Gram-negative, halophilic, aerobic, rod-shaped, oil-degrading marine bacterium that is found in low abundances in unpolluted environments in the upper layers of the ocean, but quickly becomes the predominant microbe in oil-contaminated open oceans and coastal waters when nitrogen and phosphorus are not limiting [2]. When conditions in these moderately halophilic environments are right, ''Alcanivorax'' may make up 80-90% of the oil-degrading microbes present in the area [4]. It is described as a non-motile bactertium which is true for species such as ''Alcanivorax borkumensis'', but other species such as ''Alcanivorax venustensis'' were described to be motile by polar flagella [1]. The optimial conditions described for A.borkumensis growth include temperatures in the range of 20-30 degrees celsius, and a NaCl concentration of 3-10%.<br />
<br />
As a result of their profound ability to degrade and live predominately on alkanes, as well as to become the dominant microbes in oil-contaminated areas, ''Alcanivorax'' plays a huge role in the biological cleanup of oil-contaminated environments. These oil-contaminated environments in the ocean are largely due to anthropogenic sources such as oil spills caused by tankers accidents (Figure 2), and cause serious ecological damage to plants and animals on the coast as well as other inhabitants of the ocean. Microbes such as ''Alcanivorax'' provide a major route for the breakdown of these pollutants, and demonstrate how marine bacteria keep the environment in check. Of all the ''Alcanivorax'' species and other oil-degrading microbes, ''Alcanivorax borkumensis'' is one of the most important worldwide due to the fact it produces a wide variety of very efficient oil-degrading enzymes. With this knowledge, ''A. borkumensis'' could provide a useful tool for bioremediation of oil spills.<br />
<br />
==Genome Structure==<br />
[[Image:Lorenzo.gif|thumbnail|200px|Figure 3. Mechanisms for oil degradation and survival encoded by the ''A. borkumensis SK2'' genome. Image from Victor de Lorenzo[http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html]]]<br />
<br />
The ''Alcanivorax borkumensis'' strain SK2, isolated from a seawater sediment sample in the North Sea at a site located near the Isle of Borkum, was the first hydrocarbonoclastic bacterium to be sequenced and was completed by Susanne Schneiker et al. It's genome consists of a single circular chromosome with 3,120,143 base pairs with an average G+C content of 54.7%. The genomic analysis of ''A. borkumensis SK2'' revealed several new insights into the bacterium's role for (i) n-alkane degradation (which includes metabolism, biosurfactant production and biofilm production), (ii) it's system for capturing or scavenging the small amounts of nitrogen, phosphorous, sulfur, and other elements in a nutrient-poor marine environment which allows for more efficient alkane degradation due to their main limitation of nutrient availability, (iii) as well as means for coping with stress factors such as high salt contents and high UV radiation since it thrives mostly in the upper layers in the ocean where UV light is encountered (Figure 3).<br />
<br />
It's genome encodes several systems for the catabolism of hydrocarbons which allow the bacertium to degrade all sorts of alkanes such as AlkB1 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C5-C12, and AlkB2 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C8 to C16. Both these systems are located close to the origin of replication of the chromosome. ''A. borkumensis'' is also able to degrade alkanes up to C32, branched aliphatic hydrocarbons, isoprenoid hydrocarbons such as phytane, as well as alkylarenes and alkylcycloalkanes. Thus, the genome encodes for a broad spectrum of systems for the catabolism of hydrocarbons, giving it a competitive advantage over other oil-degrading marine microbial communities. To deal with the damaging effects of UV light, ''A. borkumensis'' has a number of genes that reduce the damage. These include the full genes for DNA alkylation, recombinational and nucleotide excision repair, base excision repair, as well as the SOS response [4].<br />
<br />
==Cell Structure, Metabolism and Life Cycle==<br />
''Alcanivorax borkumensis'', a Gram-negative, rod-shaped chemoorganotroph, is able to use n-alkanes as its principle carbon and energy source by use of the broad spectrum of oil-degrading enzymes it possesses, but they can also use a limited number of organic compounds such as aliphatic hydrocarbons, volatile fatty acids, and pyruvate. However, it cannot utilize carbon sources such as sugars or amino acids. Cells grown with pyruvate were observed to be 2.0-3.0 micrometers in length and 0.4-07 micrometers in diameter, however, cells were shorter (1.0-1.5 micrometers in length) when cells were grown with n-alkanes [5]. When the slow growing ''A. borkumensis'' uses n-alkanes exclusively, the microbes produce extracellular and membrane-bound surface-active glucose lipids called biosurfactants. These biosurfactants reduce the surface tension of water from 72 to 29 dynes/cm and act as natural emulsifiers which enhances the break up of oil-in-water emulsions which prevent degradation of alkanes [4,5]. Due to the low solubility of oil in water, most oil degradation takes place at the oil-water interface where ''A. borkumensis'' attaches and forms a biofilm around the oil droplets as depicted in Figure 3.<br />
<br />
==Ecology and Pathogenesis==<br />
Habitat; symbiosis; biogeochemical significance; contributions to environment.<br />
<br />
==References==<br />
[1] [http://ijs.sgmjournals.org/cgi/content/abstract/53/1/331 Fernandez-Martinez, Javier, Maria J. Pujalte, Jesus Garcia-Martinez, Manuel Mata, Esperanza Garay, and Francisco Rodriguez-Valera. "Description of ''Alcanivorax Venustensis'' sp. nov. and Reclassification of ''Fundibacter Jadensis'' DSM 12178T (Bruns and Berthe-Corti 1999) As ''Alcanivorax Jadensis'' comb. nov., Members of the Emended Genus ''Alcanivorax''." International Journal of Systematic and Evolutionary Microbiology 53 (2003): 331-338.]<br />
<br />
[2] [http://www.blackwell-synergy.com/doi/pdf/10.1046/j.1468-2920.2003.00468.x Hara, Akihiro, Kazuaki Syutsubo, and Shigeaki Harayama. "''Alcanivorax'' Which Prevails In Oil-contaminated Seawater Exhibits Broad Substrate Specificity For Alkane Degradation." Environmental Microbiology 5.9 (2003): 746-753.]<br />
<br />
[3] [http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html Lorenzo, Víctor De. "Blueprint of an Oil-eating Bacterium." Nature Biotechnology 24 (2006): 952-953.]<br />
<br />
[4] [http://www.ncbi.nlm.nih.gov/pubmed/16878126 Schneiker, S. et al. "Genome Sequence of the Ubiquitous Hydrocarbon-degrading Marine Bacterium A''lcanivorax Borkumensis''." Nature Biotechnology 24 (2006): 997-1004.]<br />
<br />
[5] [http://ijs.sgmjournals.org/cgi/content/abstract/48/2/339 Yakimov, Michail M., Peter N. Golyshin, Siegmund Lang, Edward R. B. Moore, Wolf-Rainer Abraham, Heinrich Lunsdorf, and Kenneth N. Timmis. "''Alcanivorax Borkumensis'' gen. nov., sp. nov., A New, Hydrocarbon-degrading And Surfactant-producing Marine Bacterium." International Journal of Systematic Bacteriology 48 (1998): 339-348.]<br />
<br />
==Author==<br />
Page authored by Andrew Buss, student of [http://www.kbs.msu.edu/faculty/lennon/ Prof. Jay Lennon] at Michigan State University.</div>Bussand1https://microbewiki.kenyon.edu/index.php?title=Alcanivorax&diff=29760Alcanivorax2008-04-06T21:05:37Z<p>Bussand1: /* Genome Structure */</p>
<hr />
<div>[[Image:AlcVorax PROCARYOTES01b-1.JPG|thumbnail|300px|Figure 1. ''Alcanivorax borkumensis''. Image from Helmholtz Centre for Infection Research[http://www.helmholtz-hzi.de/en/news_public_relation/press_releases/view/article/complete/oil_tanker_accidents_as_a_source_of_food/]]]<br />
<br />
==Classification==<br />
<br />
'''Bacteria'''; Phylum: '''Proteobacteria'''; Class: '''Gammaproteobacteria'''; Order: '''Oceanospirillales'''; Family: '''Alcanivoracaceae'''<br />
<br />
===Species===<br />
<br />
{|<br />
| height="10" bgcolor="#FFDF95" |<br />
'''NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Tree&id=2&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy]'''<br />
|}<br />
<br />
*''Alcanivorax balearicum''<br />
*''Alcanivorax borkumensis''<br />
*''Alcanivorax dieselolei''<br />
*''Alcanivorax indicus''<br />
*''Alcanivorax jadensis''<br />
*''Alcanivorax venustensis''<br />
<br />
==Description and Significance==<br />
[[Image:PWS tanker oil spill.jpg|thumbnail|200px|Figure 2. Supertanker Exxon Valdez grounded on Bligh Reef which released 11 million gallons of crude oil into the water. This oil-contaminated seawater is the preferred habitat for ''Alcanivorax''. Image from USGS[http://menlocampus.wr.usgs.gov/50years/accomplishments/oil.html]]]<br />
<br />
''Alcanivorax'', first described in 1998, is a Gram-negative, halophilic, aerobic, rod-shaped, oil-degrading marine bacterium that is found in low abundances in unpolluted environments in the upper layers of the ocean, but quickly becomes the predominant microbe in oil-contaminated open oceans and coastal waters when nitrogen and phosphorus are not limiting [2]. When conditions in these moderately halophilic environments are right, ''Alcanivorax'' may make up 80-90% of the oil-degrading microbes present in the area [4]. It is described as a non-motile bactertium which is true for species such as ''Alcanivorax borkumensis'', but other species such as ''Alcanivorax venustensis'' were described to be motile by polar flagella [1]. The optimial conditions described for A.borkumensis growth include temperatures in the range of 20-30 degrees celsius, and a NaCl concentration of 3-10%.<br />
<br />
As a result of their profound ability to degrade and live predominately on alkanes, as well as to become the dominant microbes in oil-contaminated areas, ''Alcanivorax'' plays a huge role in the biological cleanup of oil-contaminated environments. These oil-contaminated environments in the ocean are largely due to anthropogenic sources such as oil spills caused by tankers accidents (Figure 2), and cause serious ecological damage to plants and animals on the coast as well as other inhabitants of the ocean. Microbes such as ''Alcanivorax'' provide a major route for the breakdown of these pollutants, and demonstrate how marine bacteria keep the environment in check. Of all the ''Alcanivorax'' species and other oil-degrading microbes, ''Alcanivorax borkumensis'' is one of the most important worldwide due to the fact it produces a wide variety of very efficient oil-degrading enzymes. With this knowledge, ''A. borkumensis'' could provide a useful tool for bioremediation of oil spills.<br />
<br />
==Genome Structure==<br />
[[Image:Lorenzo.gif|thumbnail|200px|Figure 3. Mechanisms for oil degradation and survival encoded by the ''A. borkumensis SK2'' genome. Image from Victor de Lorenzo[http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html]]]<br />
<br />
The ''Alcanivorax borkumensis'' strain SK2, isolated from a seawater sediment sample in the North Sea at a site located near the Isle of Borkum, was the first hydrocarbonoclastic bacterium to be sequenced and was completed by Susanne Schneiker et al. It's genome consists of a single circular chromosome with 3,120,143 base pairs with an average G+C content of 54.7%. The genomic analysis of ''A. borkumensis SK2'' revealed several new insights into the bacterium's role for (i) n-alkane degradation (which includes metabolism, biosurfactant production and biofilm production), (ii) it's system for capturing or scavenging the small amounts of nitrogen, phosphorous, sulfur, and other elements in a nutrient-poor marine environment which allows for more efficient alkane degradation due to their main limitation of nutrient availability, (iii) as well as means for coping with stress factors such as high salt contents and high UV radiation since it thrives mostly in the upper layers in the ocean where UV light is encountered (Figure 3).<br />
<br />
It's genome encodes several systems for the catabolism of hydrocarbons which allow the bacertium to degrade all sorts of alkanes such as AlkB1 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C5-C12, and AlkB2 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C8 to C16. Both these systems are located close to the origin of replication of the chromosome. ''A. borkumensis'' is also able to degrade alkanes up to C32, branched aliphatic hydrocarbons, isoprenoid hydrocarbons such as phytane, as well as alkylarenes and alkylcycloalkanes. Thus, the genome encodes for a broad spectrum of systems for the catabolism of hydrocarbons, giving it a competitive advantage over other oil-degrading marine microbial communities. To deal with the damaging effects of UV light, ''A. borkumensis'' has a number of genes that reduce the damage. These include the full genes for DNA alkylation, recombinational and nucleotide excision repair, base excision repair, as well as the SOS response [4].<br />
<br />
==Cell Structure, Metabolism and Life Cycle==<br />
''Alcanivorax borkumensis'', a Gram-negative, rod-shaped chemoorganotroph, is able to use n-alkanes as its principle carbon and energy source by use of the broad spectrum of oil-degrading enzymes it possesses, but they can also use a limited number of organic compounds such as aliphatic hydrocarbons, volatile fatty acids, and pyruvate. However, it cannot utilize carbon sources such as sugars or amino acids. Cells grown with pyruvate were observed to be 2.0-3.0 micrometers in length and 0.4-07 micrometers in diameter, however, cells were shorter (1.0-1.5 micrometers in length) when cells were grown with n-alkanes [5]. When the slow growing ''A. borkumensis'' uses n-alkanes exclusively, the microbes produce extracellular and membrane-bound surface-active glucose lipids called biosurfactants. These biosurfactants reduce the surface tension of water from 72 to 29 dynes/cm and act as natural emulsifiers which enhances the break up of oil-in-water emulsions which prevent degradation of alkanes [4,5]. Due to the low solubility of oil in water, most oil degradation takes place at the oil-water interface where ''A. borkumensis'' attaches and forms a biofilm around the oil droplets as depicted in Figure 3.<br />
<br />
==Ecology and Pathogenesis==<br />
Habitat; symbiosis; biogeochemical significance; contributions to environment.<br><br />
If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.<br><br><br />
<br />
==References==<br />
[1] [http://ijs.sgmjournals.org/cgi/content/abstract/53/1/331 Fernandez-Martinez, Javier, Maria J. Pujalte, Jesus Garcia-Martinez, Manuel Mata, Esperanza Garay, and Francisco Rodriguez-Valera. "Description of ''Alcanivorax Venustensis'' sp. nov. and Reclassification of ''Fundibacter Jadensis'' DSM 12178T (Bruns and Berthe-Corti 1999) As ''Alcanivorax Jadensis'' comb. nov., Members of the Emended Genus ''Alcanivorax''." International Journal of Systematic and Evolutionary Microbiology 53 (2003): 331-338.]<br />
<br />
[2] [http://www.blackwell-synergy.com/doi/pdf/10.1046/j.1468-2920.2003.00468.x Hara, Akihiro, Kazuaki Syutsubo, and Shigeaki Harayama. "''Alcanivorax'' Which Prevails In Oil-contaminated Seawater Exhibits Broad Substrate Specificity For Alkane Degradation." Environmental Microbiology 5.9 (2003): 746-753.]<br />
<br />
[3] [http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html Lorenzo, Víctor De. "Blueprint of an Oil-eating Bacterium." Nature Biotechnology 24 (2006): 952-953.]<br />
<br />
[4] [http://www.ncbi.nlm.nih.gov/pubmed/16878126 Schneiker, S. et al. "Genome Sequence of the Ubiquitous Hydrocarbon-degrading Marine Bacterium A''lcanivorax Borkumensis''." Nature Biotechnology 24 (2006): 997-1004.]<br />
<br />
[5] [http://ijs.sgmjournals.org/cgi/content/abstract/48/2/339 Yakimov, Michail M., Peter N. Golyshin, Siegmund Lang, Edward R. B. Moore, Wolf-Rainer Abraham, Heinrich Lunsdorf, and Kenneth N. Timmis. "''Alcanivorax Borkumensis'' gen. nov., sp. nov., A New, Hydrocarbon-degrading And Surfactant-producing Marine Bacterium." International Journal of Systematic Bacteriology 48 (1998): 339-348.]<br />
<br />
==Author==<br />
Page authored by Andrew Buss, student of [http://www.kbs.msu.edu/faculty/lennon/ Prof. Jay Lennon] at Michigan State University.</div>Bussand1https://microbewiki.kenyon.edu/index.php?title=Alcanivorax&diff=29759Alcanivorax2008-04-06T21:04:42Z<p>Bussand1: </p>
<hr />
<div>[[Image:AlcVorax PROCARYOTES01b-1.JPG|thumbnail|300px|Figure 1. ''Alcanivorax borkumensis''. Image from Helmholtz Centre for Infection Research[http://www.helmholtz-hzi.de/en/news_public_relation/press_releases/view/article/complete/oil_tanker_accidents_as_a_source_of_food/]]]<br />
<br />
==Classification==<br />
<br />
'''Bacteria'''; Phylum: '''Proteobacteria'''; Class: '''Gammaproteobacteria'''; Order: '''Oceanospirillales'''; Family: '''Alcanivoracaceae'''<br />
<br />
===Species===<br />
<br />
{|<br />
| height="10" bgcolor="#FFDF95" |<br />
'''NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Tree&id=2&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy]'''<br />
|}<br />
<br />
*''Alcanivorax balearicum''<br />
*''Alcanivorax borkumensis''<br />
*''Alcanivorax dieselolei''<br />
*''Alcanivorax indicus''<br />
*''Alcanivorax jadensis''<br />
*''Alcanivorax venustensis''<br />
<br />
==Description and Significance==<br />
[[Image:PWS tanker oil spill.jpg|thumbnail|200px|Figure 2. Supertanker Exxon Valdez grounded on Bligh Reef which released 11 million gallons of crude oil into the water. This oil-contaminated seawater is the preferred habitat for ''Alcanivorax''. Image from USGS[http://menlocampus.wr.usgs.gov/50years/accomplishments/oil.html]]]<br />
<br />
''Alcanivorax'', first described in 1998, is a Gram-negative, halophilic, aerobic, rod-shaped, oil-degrading marine bacterium that is found in low abundances in unpolluted environments in the upper layers of the ocean, but quickly becomes the predominant microbe in oil-contaminated open oceans and coastal waters when nitrogen and phosphorus are not limiting [2]. When conditions in these moderately halophilic environments are right, ''Alcanivorax'' may make up 80-90% of the oil-degrading microbes present in the area [4]. It is described as a non-motile bactertium which is true for species such as ''Alcanivorax borkumensis'', but other species such as ''Alcanivorax venustensis'' were described to be motile by polar flagella [1]. The optimial conditions described for A.borkumensis growth include temperatures in the range of 20-30 degrees celsius, and a NaCl concentration of 3-10%.<br />
<br />
As a result of their profound ability to degrade and live predominately on alkanes, as well as to become the dominant microbes in oil-contaminated areas, ''Alcanivorax'' plays a huge role in the biological cleanup of oil-contaminated environments. These oil-contaminated environments in the ocean are largely due to anthropogenic sources such as oil spills caused by tankers accidents (Figure 2), and cause serious ecological damage to plants and animals on the coast as well as other inhabitants of the ocean. Microbes such as ''Alcanivorax'' provide a major route for the breakdown of these pollutants, and demonstrate how marine bacteria keep the environment in check. Of all the ''Alcanivorax'' species and other oil-degrading microbes, ''Alcanivorax borkumensis'' is one of the most important worldwide due to the fact it produces a wide variety of very efficient oil-degrading enzymes. With this knowledge, ''A. borkumensis'' could provide a useful tool for bioremediation of oil spills.<br />
<br />
==Genome Structure==<br />
[[Image:Lorenzo.gif|thumbnail|200px|Figure 3. Mechanisms for oil degradation and survival encoded by the ''A. borkumensis SK2'' genome. Image from Victor de Lorenzo[http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html]]]<br />
<br />
The ''Alcanivorax borkumensis'' strain SK2, isolated from a seawater sediment sample in the North Sea at a site located near the Isle of Borkum, was the first hydrocarbonoclastic bacterium to be sequenced and was completed by Susanne Schneiker et al. It's genome consists of a single circular chromosome with 3,120,143 base pairs with an average G+C content of 54.7%. The genomic analysis of ''A. borkumensis SK2'' revealed several new insights into the bacterium's role for (i) n-alkane degradation (which includes metabolism, biosurfactant production and biofilm production), (ii) it's system for capturing or scavenging the small amounts of nitrogen, phosphorous, sulfur, and other elements in a nutrient-poor marine environment which allows for more efficient alkane degradation due to their main limitation of nutrient availability, (iii) as well as means for coping with stress factors such as high salt contents and high UV radiation since it thrives mostly in the upper layers in the ocean where UV light is encountered (Figure 3). It's genome encodes several systems for the catabolism of hydrocarbons which allow the bacertium to degrade all sorts of alkanes such as AlkB1 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C5-C12, and AlkB2 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C8 to C16. Both these systems are located close to the origin of replication of the chromosome. ''A. borkumensis'' is also able to degrade alkanes up to C32, branched aliphatic hydrocarbons, isoprenoid hydrocarbons such as phytane, as well as alkylarenes and alkylcycloalkanes. Thus, the genome encodes for a broad spectrum of systems for the catabolism of hydrocarbons, giving it a competitive advantage over other oil-degrading marine microbial communities. To deal with the damaging effects of UV light, ''A. borkumensis'' has a number of genes that reduce the damage. These include the full genes for DNA alkylation, recombinational and nucleotide excision repair, base excision repair, as well as the SOS response [4].<br />
<br />
==Cell Structure, Metabolism and Life Cycle==<br />
''Alcanivorax borkumensis'', a Gram-negative, rod-shaped chemoorganotroph, is able to use n-alkanes as its principle carbon and energy source by use of the broad spectrum of oil-degrading enzymes it possesses, but they can also use a limited number of organic compounds such as aliphatic hydrocarbons, volatile fatty acids, and pyruvate. However, it cannot utilize carbon sources such as sugars or amino acids. Cells grown with pyruvate were observed to be 2.0-3.0 micrometers in length and 0.4-07 micrometers in diameter, however, cells were shorter (1.0-1.5 micrometers in length) when cells were grown with n-alkanes [5]. When the slow growing ''A. borkumensis'' uses n-alkanes exclusively, the microbes produce extracellular and membrane-bound surface-active glucose lipids called biosurfactants. These biosurfactants reduce the surface tension of water from 72 to 29 dynes/cm and act as natural emulsifiers which enhances the break up of oil-in-water emulsions which prevent degradation of alkanes [4,5]. Due to the low solubility of oil in water, most oil degradation takes place at the oil-water interface where ''A. borkumensis'' attaches and forms a biofilm around the oil droplets as depicted in Figure 3.<br />
<br />
==Ecology and Pathogenesis==<br />
Habitat; symbiosis; biogeochemical significance; contributions to environment.<br><br />
If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.<br><br><br />
<br />
==References==<br />
[1] [http://ijs.sgmjournals.org/cgi/content/abstract/53/1/331 Fernandez-Martinez, Javier, Maria J. Pujalte, Jesus Garcia-Martinez, Manuel Mata, Esperanza Garay, and Francisco Rodriguez-Valera. "Description of ''Alcanivorax Venustensis'' sp. nov. and Reclassification of ''Fundibacter Jadensis'' DSM 12178T (Bruns and Berthe-Corti 1999) As ''Alcanivorax Jadensis'' comb. nov., Members of the Emended Genus ''Alcanivorax''." International Journal of Systematic and Evolutionary Microbiology 53 (2003): 331-338.]<br />
<br />
[2] [http://www.blackwell-synergy.com/doi/pdf/10.1046/j.1468-2920.2003.00468.x Hara, Akihiro, Kazuaki Syutsubo, and Shigeaki Harayama. "''Alcanivorax'' Which Prevails In Oil-contaminated Seawater Exhibits Broad Substrate Specificity For Alkane Degradation." Environmental Microbiology 5.9 (2003): 746-753.]<br />
<br />
[3] [http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html Lorenzo, Víctor De. "Blueprint of an Oil-eating Bacterium." Nature Biotechnology 24 (2006): 952-953.]<br />
<br />
[4] [http://www.ncbi.nlm.nih.gov/pubmed/16878126 Schneiker, S. et al. "Genome Sequence of the Ubiquitous Hydrocarbon-degrading Marine Bacterium A''lcanivorax Borkumensis''." Nature Biotechnology 24 (2006): 997-1004.]<br />
<br />
[5] [http://ijs.sgmjournals.org/cgi/content/abstract/48/2/339 Yakimov, Michail M., Peter N. Golyshin, Siegmund Lang, Edward R. B. Moore, Wolf-Rainer Abraham, Heinrich Lunsdorf, and Kenneth N. Timmis. "''Alcanivorax Borkumensis'' gen. nov., sp. nov., A New, Hydrocarbon-degrading And Surfactant-producing Marine Bacterium." International Journal of Systematic Bacteriology 48 (1998): 339-348.]<br />
<br />
==Author==<br />
Page authored by Andrew Buss, student of [http://www.kbs.msu.edu/faculty/lennon/ Prof. Jay Lennon] at Michigan State University.</div>Bussand1https://microbewiki.kenyon.edu/index.php?title=Alcanivorax&diff=29758Alcanivorax2008-04-06T20:56:48Z<p>Bussand1: /* Cell Structure, Metabolism and Life Cycle */</p>
<hr />
<div>[[Image:AlcVorax PROCARYOTES01b-1.JPG|thumbnail|300px|Figure 1. ''Alcanivorax borkumensis''. Image from Helmholtz Centre for Infection Research[http://www.helmholtz-hzi.de/en/news_public_relation/press_releases/view/article/complete/oil_tanker_accidents_as_a_source_of_food/]]]<br />
<br />
==Classification==<br />
<br />
'''Bacteria'''; Phylum: '''Proteobacteria'''; Class: '''Gammaproteobacteria'''; Order: '''Oceanospirillales'''; Family: '''Alcanivoracaceae'''<br />
<br />
===Species===<br />
<br />
{|<br />
| height="10" bgcolor="#FFDF95" |<br />
'''NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Tree&id=2&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy]'''<br />
|}<br />
<br />
*''Alcanivorax balearicum''<br />
*''Alcanivorax borkumensis''<br />
*''Alcanivorax dieselolei''<br />
*''Alcanivorax indicus''<br />
*''Alcanivorax jadensis''<br />
*''Alcanivorax venustensis''<br />
<br />
==Description and Significance==<br />
[[Image:PWS tanker oil spill.jpg|thumbnail|200px|Figure 2. Supertanker Exxon Valdez grounded on Bligh Reef which released 11 million gallons of crude oil into the water. This oil-contaminated seawater is the preferred habitat for ''Alcanivorax''. Image from USGS[http://menlocampus.wr.usgs.gov/50years/accomplishments/oil.html]]]<br />
<br />
''Alcanivorax'', first described in 1998, is a Gram-negative, halophilic, aerobic, rod-shaped, oil-degrading marine bacterium that is found in low abundances in unpolluted environments in the upper layers of the ocean, but quickly becomes the predominant microbe in oil-contaminated open oceans and coastal waters when nitrogen and phosphorus are not limiting [2]. When conditions in these moderately halophilic environments are right, ''Alcanivorax'' may make up 80-90% of the oil-degrading microbes present in the area [4]. It is described as a non-motile bactertium which is true for species such as ''Alcanivorax borkumensis'', but other species such as ''Alcanivorax venustensis'' were described to be motile by polar flagella [1]. The optimial conditions described for A.borkumensis growth include temperatures in the range of 20-30 degrees celsius, and a NaCl concentration of 3-10%. Cells grown with pyruvate were observed to be 2.0-3.0 micrometers in length and 0.4-07 micrometers in diameter, however, cells were shorter (1.0-1.5 micrometers in length) when cells were grown with n-alkanes [5].<br />
<br />
As a result of their profound ability to degrade and live predominately on alkanes, as well as to become the dominant microbes in oil-contaminated areas, ''Alcanivorax'' plays a huge role in the biological cleanup of oil-contaminated environments. These oil-contaminated environments in the ocean are largely due to anthropogenic sources such as oil spills caused by tankers accidents (Figure 2), and cause serious ecological damage to plants and animals on the coast as well as other inhabitants of the ocean. Microbes such as ''Alcanivorax'' provide a major route for the breakdown of these pollutants, and demonstrate how marine bacteria keep the environment in check. Of all the ''Alcanivorax'' species and other oil-degrading microbes, ''Alcanivorax borkumensis'' is one of the most important worldwide due to the fact it produces a wide variety of very efficient oil-degrading enzymes. With this knowledge, ''A. borkumensis'' could provide a useful tool for bioremediation of oil spills.<br />
<br />
==Genome Structure==<br />
[[Image:Lorenzo.gif|thumbnail|200px|Figure 3. Mechanisms for oil degradation and survival encoded by the ''A. borkumensis SK2'' genome. Image from Victor de Lorenzo[http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html]]]<br />
<br />
The ''Alcanivorax borkumensis'' strain SK2, isolated from a seawater sediment sample in the North Sea at a site located near the Isle of Borkum, was the first hydrocarbonoclastic bacterium to be sequenced and was completed by Susanne Schneiker et al. It's genome consists of a single circular chromosome with 3,120,143 base pairs with an average G+C content of 54.7%. The genomic analysis of ''A. borkumensis SK2'' revealed several new insights into the bacterium's role for (i) n-alkane degradation (which includes metabolism, biosurfactant production and biofilm production), (ii) it's system for capturing or scavenging the small amounts of nitrogen, phosphorous, sulfur, and other elements in a nutrient-poor marine environment which allows for more efficient alkane degradation due to their main limitation of nutrient availability, (iii) as well as means for coping with stress factors such as high salt contents and high UV radiation since it thrives mostly in the upper layers in the ocean where UV light is encountered (Figure 3). It's genome encodes several systems for the catabolism of hydrocarbons which allow the bacertium to degrade all sorts of alkanes such as AlkB1 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C5-C12, and AlkB2 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C8 to C16. Both these systems are located close to the origin of replication of the chromosome. ''A. borkumensis'' is also able to degrade alkanes up to C32, branched aliphatic hydrocarbons, isoprenoid hydrocarbons such as phytane, as well as alkylarenes and alkylcycloalkanes. Thus, the genome encodes for a broad spectrum of systems for the catabolism of hydrocarbons, giving it a competitive advantage over other oil-degrading marine microbial communities. To deal with the damaging effects of UV light, ''A. borkumensis'' has a number of genes that reduce the damage. These include the full genes for DNA alkylation, recombinational and nucleotide excision repair, base excision repair, as well as the SOS response [4].<br />
<br />
==Cell Structure, Metabolism and Life Cycle==<br />
''Alcanivorax borkumensis'', a chemoorganotroph, is able to use n-alkanes as its principle carbon and energy source by use of the broad spectrum of oil-degrading enzymes it possesses, but they can also use a limited number of organic compounds such as aliphatic hydrocarbons, volatile fatty acids, and pyruvate. However, it cannot utilize carbon sources such as sugars or amino acids. When the slow growing ''A. borkumensis'' uses n-alkanes exclusively, the microbes produce extracellular and membrane-bound surface-active glucose lipids called biosurfactants. These biosurfactants reduce the surface tension of water from 72 to 29 dynes/cm and act as natural emulsifiers which enhances the break up of oil-in-water emulsions which prevent degradation of alkanes [4,5]. Due to the low solubility of oil in water, most oil degradation takes place at the oil-water interface where ''A. borkumensis'' attaches and forms a biofilm around the oil droplets as depicted in Figure 3.<br />
<br />
==Ecology and Pathogenesis==<br />
Habitat; symbiosis; biogeochemical significance; contributions to environment.<br><br />
If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.<br><br><br />
<br />
==References==<br />
[1] [http://ijs.sgmjournals.org/cgi/content/abstract/53/1/331 Fernandez-Martinez, Javier, Maria J. Pujalte, Jesus Garcia-Martinez, Manuel Mata, Esperanza Garay, and Francisco Rodriguez-Valera. "Description of ''Alcanivorax Venustensis'' sp. nov. and Reclassification of ''Fundibacter Jadensis'' DSM 12178T (Bruns and Berthe-Corti 1999) As ''Alcanivorax Jadensis'' comb. nov., Members of the Emended Genus ''Alcanivorax''." International Journal of Systematic and Evolutionary Microbiology 53 (2003): 331-338.]<br />
<br />
[2] [http://www.blackwell-synergy.com/doi/pdf/10.1046/j.1468-2920.2003.00468.x Hara, Akihiro, Kazuaki Syutsubo, and Shigeaki Harayama. "''Alcanivorax'' Which Prevails In Oil-contaminated Seawater Exhibits Broad Substrate Specificity For Alkane Degradation." Environmental Microbiology 5.9 (2003): 746-753.]<br />
<br />
[3] [http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html Lorenzo, Víctor De. "Blueprint of an Oil-eating Bacterium." Nature Biotechnology 24 (2006): 952-953.]<br />
<br />
[4] [http://www.ncbi.nlm.nih.gov/pubmed/16878126 Schneiker, S. et al. "Genome Sequence of the Ubiquitous Hydrocarbon-degrading Marine Bacterium A''lcanivorax Borkumensis''." Nature Biotechnology 24 (2006): 997-1004.]<br />
<br />
[5] [http://ijs.sgmjournals.org/cgi/content/abstract/48/2/339 Yakimov, Michail M., Peter N. Golyshin, Siegmund Lang, Edward R. B. Moore, Wolf-Rainer Abraham, Heinrich Lunsdorf, and Kenneth N. Timmis. "''Alcanivorax Borkumensis'' gen. nov., sp. nov., A New, Hydrocarbon-degrading And Surfactant-producing Marine Bacterium." International Journal of Systematic Bacteriology 48 (1998): 339-348.]<br />
<br />
==Author==<br />
Page authored by Andrew Buss, student of [http://www.kbs.msu.edu/faculty/lennon/ Prof. Jay Lennon] at Michigan State University.</div>Bussand1https://microbewiki.kenyon.edu/index.php?title=Alcanivorax&diff=29757Alcanivorax2008-04-06T20:56:01Z<p>Bussand1: /* Description and Significance */</p>
<hr />
<div>[[Image:AlcVorax PROCARYOTES01b-1.JPG|thumbnail|300px|Figure 1. ''Alcanivorax borkumensis''. Image from Helmholtz Centre for Infection Research[http://www.helmholtz-hzi.de/en/news_public_relation/press_releases/view/article/complete/oil_tanker_accidents_as_a_source_of_food/]]]<br />
<br />
==Classification==<br />
<br />
'''Bacteria'''; Phylum: '''Proteobacteria'''; Class: '''Gammaproteobacteria'''; Order: '''Oceanospirillales'''; Family: '''Alcanivoracaceae'''<br />
<br />
===Species===<br />
<br />
{|<br />
| height="10" bgcolor="#FFDF95" |<br />
'''NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Tree&id=2&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy]'''<br />
|}<br />
<br />
*''Alcanivorax balearicum''<br />
*''Alcanivorax borkumensis''<br />
*''Alcanivorax dieselolei''<br />
*''Alcanivorax indicus''<br />
*''Alcanivorax jadensis''<br />
*''Alcanivorax venustensis''<br />
<br />
==Description and Significance==<br />
[[Image:PWS tanker oil spill.jpg|thumbnail|200px|Figure 2. Supertanker Exxon Valdez grounded on Bligh Reef which released 11 million gallons of crude oil into the water. This oil-contaminated seawater is the preferred habitat for ''Alcanivorax''. Image from USGS[http://menlocampus.wr.usgs.gov/50years/accomplishments/oil.html]]]<br />
<br />
''Alcanivorax'', first described in 1998, is a Gram-negative, halophilic, aerobic, rod-shaped, oil-degrading marine bacterium that is found in low abundances in unpolluted environments in the upper layers of the ocean, but quickly becomes the predominant microbe in oil-contaminated open oceans and coastal waters when nitrogen and phosphorus are not limiting [2]. When conditions in these moderately halophilic environments are right, ''Alcanivorax'' may make up 80-90% of the oil-degrading microbes present in the area [4]. It is described as a non-motile bactertium which is true for species such as ''Alcanivorax borkumensis'', but other species such as ''Alcanivorax venustensis'' were described to be motile by polar flagella [1]. The optimial conditions described for A.borkumensis growth include temperatures in the range of 20-30 degrees celsius, and a NaCl concentration of 3-10%. Cells grown with pyruvate were observed to be 2.0-3.0 micrometers in length and 0.4-07 micrometers in diameter, however, cells were shorter (1.0-1.5 micrometers in length) when cells were grown with n-alkanes [5].<br />
<br />
As a result of their profound ability to degrade and live predominately on alkanes, as well as to become the dominant microbes in oil-contaminated areas, ''Alcanivorax'' plays a huge role in the biological cleanup of oil-contaminated environments. These oil-contaminated environments in the ocean are largely due to anthropogenic sources such as oil spills caused by tankers accidents (Figure 2), and cause serious ecological damage to plants and animals on the coast as well as other inhabitants of the ocean. Microbes such as ''Alcanivorax'' provide a major route for the breakdown of these pollutants, and demonstrate how marine bacteria keep the environment in check. Of all the ''Alcanivorax'' species and other oil-degrading microbes, ''Alcanivorax borkumensis'' is one of the most important worldwide due to the fact it produces a wide variety of very efficient oil-degrading enzymes. With this knowledge, ''A. borkumensis'' could provide a useful tool for bioremediation of oil spills.<br />
<br />
==Genome Structure==<br />
[[Image:Lorenzo.gif|thumbnail|200px|Figure 3. Mechanisms for oil degradation and survival encoded by the ''A. borkumensis SK2'' genome. Image from Victor de Lorenzo[http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html]]]<br />
<br />
The ''Alcanivorax borkumensis'' strain SK2, isolated from a seawater sediment sample in the North Sea at a site located near the Isle of Borkum, was the first hydrocarbonoclastic bacterium to be sequenced and was completed by Susanne Schneiker et al. It's genome consists of a single circular chromosome with 3,120,143 base pairs with an average G+C content of 54.7%. The genomic analysis of ''A. borkumensis SK2'' revealed several new insights into the bacterium's role for (i) n-alkane degradation (which includes metabolism, biosurfactant production and biofilm production), (ii) it's system for capturing or scavenging the small amounts of nitrogen, phosphorous, sulfur, and other elements in a nutrient-poor marine environment which allows for more efficient alkane degradation due to their main limitation of nutrient availability, (iii) as well as means for coping with stress factors such as high salt contents and high UV radiation since it thrives mostly in the upper layers in the ocean where UV light is encountered (Figure 3). It's genome encodes several systems for the catabolism of hydrocarbons which allow the bacertium to degrade all sorts of alkanes such as AlkB1 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C5-C12, and AlkB2 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C8 to C16. Both these systems are located close to the origin of replication of the chromosome. ''A. borkumensis'' is also able to degrade alkanes up to C32, branched aliphatic hydrocarbons, isoprenoid hydrocarbons such as phytane, as well as alkylarenes and alkylcycloalkanes. Thus, the genome encodes for a broad spectrum of systems for the catabolism of hydrocarbons, giving it a competitive advantage over other oil-degrading marine microbial communities. To deal with the damaging effects of UV light, ''A. borkumensis'' has a number of genes that reduce the damage. These include the full genes for DNA alkylation, recombinational and nucleotide excision repair, base excision repair, as well as the SOS response [4].<br />
<br />
==Cell Structure, Metabolism and Life Cycle==<br />
Alcanivorax borkumensis, a chemoorganotroph, is able to use n-alkanes as its principle carbon and energy source by use of the broad spectrum of oil-degrading enzymes it possesses, but they can also use a limited number of organic compounds such as aliphatic hydrocarbons, volatile fatty acids, and pyruvate. However, it cannot utilize carbon sources such as sugars or amino acids. When the slow growing ''A. borkumensis'' uses n-alkanes exclusively, the microbes produce extracellular and membrane-bound surface-active glucose lipids called biosurfactants. These biosurfactants reduce the surface tension of water from 72 to 29 dynes/cm and act as natural emulsifiers which enhances the break up of oil-in-water emulsions which prevent degradation of alkanes [4,5]. Due to the low solubility of oil in water, most oil degradation takes place at the oil-water interface where ''A. borkumensis'' attaches and forms a biofilm around the oil droplets as depicted in Figure 3.<br />
<br />
==Ecology and Pathogenesis==<br />
Habitat; symbiosis; biogeochemical significance; contributions to environment.<br><br />
If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.<br><br><br />
<br />
==References==<br />
[1] [http://ijs.sgmjournals.org/cgi/content/abstract/53/1/331 Fernandez-Martinez, Javier, Maria J. Pujalte, Jesus Garcia-Martinez, Manuel Mata, Esperanza Garay, and Francisco Rodriguez-Valera. "Description of ''Alcanivorax Venustensis'' sp. nov. and Reclassification of ''Fundibacter Jadensis'' DSM 12178T (Bruns and Berthe-Corti 1999) As ''Alcanivorax Jadensis'' comb. nov., Members of the Emended Genus ''Alcanivorax''." International Journal of Systematic and Evolutionary Microbiology 53 (2003): 331-338.]<br />
<br />
[2] [http://www.blackwell-synergy.com/doi/pdf/10.1046/j.1468-2920.2003.00468.x Hara, Akihiro, Kazuaki Syutsubo, and Shigeaki Harayama. "''Alcanivorax'' Which Prevails In Oil-contaminated Seawater Exhibits Broad Substrate Specificity For Alkane Degradation." Environmental Microbiology 5.9 (2003): 746-753.]<br />
<br />
[3] [http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html Lorenzo, Víctor De. "Blueprint of an Oil-eating Bacterium." Nature Biotechnology 24 (2006): 952-953.]<br />
<br />
[4] [http://www.ncbi.nlm.nih.gov/pubmed/16878126 Schneiker, S. et al. "Genome Sequence of the Ubiquitous Hydrocarbon-degrading Marine Bacterium A''lcanivorax Borkumensis''." Nature Biotechnology 24 (2006): 997-1004.]<br />
<br />
[5] [http://ijs.sgmjournals.org/cgi/content/abstract/48/2/339 Yakimov, Michail M., Peter N. Golyshin, Siegmund Lang, Edward R. B. Moore, Wolf-Rainer Abraham, Heinrich Lunsdorf, and Kenneth N. Timmis. "''Alcanivorax Borkumensis'' gen. nov., sp. nov., A New, Hydrocarbon-degrading And Surfactant-producing Marine Bacterium." International Journal of Systematic Bacteriology 48 (1998): 339-348.]<br />
<br />
==Author==<br />
Page authored by Andrew Buss, student of [http://www.kbs.msu.edu/faculty/lennon/ Prof. Jay Lennon] at Michigan State University.</div>Bussand1https://microbewiki.kenyon.edu/index.php?title=Alcanivorax&diff=29755Alcanivorax2008-04-06T20:51:26Z<p>Bussand1: /* Description and Significance */</p>
<hr />
<div>[[Image:AlcVorax PROCARYOTES01b-1.JPG|thumbnail|300px|Figure 1. ''Alcanivorax borkumensis''. Image from Helmholtz Centre for Infection Research[http://www.helmholtz-hzi.de/en/news_public_relation/press_releases/view/article/complete/oil_tanker_accidents_as_a_source_of_food/]]]<br />
<br />
==Classification==<br />
<br />
'''Bacteria'''; Phylum: '''Proteobacteria'''; Class: '''Gammaproteobacteria'''; Order: '''Oceanospirillales'''; Family: '''Alcanivoracaceae'''<br />
<br />
===Species===<br />
<br />
{|<br />
| height="10" bgcolor="#FFDF95" |<br />
'''NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Tree&id=2&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy]'''<br />
|}<br />
<br />
*''Alcanivorax balearicum''<br />
*''Alcanivorax borkumensis''<br />
*''Alcanivorax dieselolei''<br />
*''Alcanivorax indicus''<br />
*''Alcanivorax jadensis''<br />
*''Alcanivorax venustensis''<br />
<br />
==Description and Significance==<br />
[[Image:PWS tanker oil spill.jpg|thumbnail|200px|Figure 2. Supertanker Exxon Valdez grounded on Bligh Reef which released 11 million gallons of crude oil into the water. This oil-contaminated seawater is the preferred habitat for ''Alcanivorax''. Image from USGS[http://menlocampus.wr.usgs.gov/50years/accomplishments/oil.html]]]<br />
<br />
''Alcanivorax'', first described in 1998, is a Gram-negative, halophilic, aerobic, rod-shaped, oil-degrading marine bacterium that is found in low abundances in unpolluted environments in the upper layers of the ocean, but quickly becomes the predominant microbe in oil-contaminated open oceans and coastal waters when nitrogen and phosphorus are not limiting [2]. When conditions in these moderately halophilic environments are right, ''Alcanivorax'' may make up 80-90% of the oil-degrading microbes present in the area [4]. It is described as a non-motile bactertium which is true for species such as ''Alcanivorax borkumensis'', but other species such as ''Alcanivorax venustensis'' were described to be motile by polar flagella [1]. The optimial conditions described for A.borkumensis growth include temperatures in the range of 20-30 degrees C, and a NaCl concentration of 3-10%. Cells grown with pyruvate were observed to be 2.0-3.0um in length and 0.4-07um in diameter, however, cells were shorter (1.0-1.5um in length) when cells were grown with n-alkanes [5].<br />
<br />
As a result of their profound ability to degrade and live predominately on alkanes, as well as to become the dominant microbes in oil-contaminated areas, ''Alcanivorax'' plays a huge role in the biological cleanup of oil-contaminated environments. These oil-contaminated environments in the ocean are largely due to anthropogenic sources such as oil spills caused by tankers accidents (Figure 2), and cause serious ecological damage to plants and animals on the coast as well as other inhabitants of the ocean. Microbes such as ''Alcanivorax'' provide a major route for the breakdown of these pollutants, and demonstrate how marine bacteria keep the environment in check. Of all the ''Alcanivorax'' species and other oil-degrading microbes, ''Alcanivorax borkumensis'' is one of the most important worldwide due to the fact it produces a wide variety of very efficient oil-degrading enzymes. With this knowledge, ''A. borkumensis'' could provide a useful tool for bioremediation of oil spills.<br />
<br />
==Genome Structure==<br />
[[Image:Lorenzo.gif|thumbnail|200px|Figure 3. Mechanisms for oil degradation and survival encoded by the ''A. borkumensis SK2'' genome. Image from Victor de Lorenzo[http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html]]]<br />
<br />
The ''Alcanivorax borkumensis'' strain SK2, isolated from a seawater sediment sample in the North Sea at a site located near the Isle of Borkum, was the first hydrocarbonoclastic bacterium to be sequenced and was completed by Susanne Schneiker et al. It's genome consists of a single circular chromosome with 3,120,143 base pairs with an average G+C content of 54.7%. The genomic analysis of ''A. borkumensis SK2'' revealed several new insights into the bacterium's role for (i) n-alkane degradation (which includes metabolism, biosurfactant production and biofilm production), (ii) it's system for capturing or scavenging the small amounts of nitrogen, phosphorous, sulfur, and other elements in a nutrient-poor marine environment which allows for more efficient alkane degradation due to their main limitation of nutrient availability, (iii) as well as means for coping with stress factors such as high salt contents and high UV radiation since it thrives mostly in the upper layers in the ocean where UV light is encountered (Figure 3). It's genome encodes several systems for the catabolism of hydrocarbons which allow the bacertium to degrade all sorts of alkanes such as AlkB1 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C5-C12, and AlkB2 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C8 to C16. Both these systems are located close to the origin of replication of the chromosome. ''A. borkumensis'' is also able to degrade alkanes up to C32, branched aliphatic hydrocarbons, isoprenoid hydrocarbons such as phytane, as well as alkylarenes and alkylcycloalkanes. Thus, the genome encodes for a broad spectrum of systems for the catabolism of hydrocarbons, giving it a competitive advantage over other oil-degrading marine microbial communities. To deal with the damaging effects of UV light, ''A. borkumensis'' has a number of genes that reduce the damage. These include the full genes for DNA alkylation, recombinational and nucleotide excision repair, base excision repair, as well as the SOS response [4].<br />
<br />
==Cell Structure, Metabolism and Life Cycle==<br />
Alcanivorax borkumensis, a chemoorganotroph, is able to use n-alkanes as its principle carbon and energy source by use of the broad spectrum of oil-degrading enzymes it possesses, but they can also use a limited number of organic compounds such as aliphatic hydrocarbons, volatile fatty acids, and pyruvate. However, it cannot utilize carbon sources such as sugars or amino acids. When the slow growing ''A. borkumensis'' uses n-alkanes exclusively, the microbes produce extracellular and membrane-bound surface-active glucose lipids called biosurfactants. These biosurfactants reduce the surface tension of water from 72 to 29 dynes/cm and act as natural emulsifiers which enhances the break up of oil-in-water emulsions which prevent degradation of alkanes [4,5]. Due to the low solubility of oil in water, most oil degradation takes place at the oil-water interface where ''A. borkumensis'' attaches and forms a biofilm around the oil droplets as depicted in Figure 3.<br />
<br />
==Ecology and Pathogenesis==<br />
Habitat; symbiosis; biogeochemical significance; contributions to environment.<br><br />
If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.<br><br><br />
<br />
==References==<br />
[1] [http://ijs.sgmjournals.org/cgi/content/abstract/53/1/331 Fernandez-Martinez, Javier, Maria J. Pujalte, Jesus Garcia-Martinez, Manuel Mata, Esperanza Garay, and Francisco Rodriguez-Valera. "Description of ''Alcanivorax Venustensis'' sp. nov. and Reclassification of ''Fundibacter Jadensis'' DSM 12178T (Bruns and Berthe-Corti 1999) As ''Alcanivorax Jadensis'' comb. nov., Members of the Emended Genus ''Alcanivorax''." International Journal of Systematic and Evolutionary Microbiology 53 (2003): 331-338.]<br />
<br />
[2] [http://www.blackwell-synergy.com/doi/pdf/10.1046/j.1468-2920.2003.00468.x Hara, Akihiro, Kazuaki Syutsubo, and Shigeaki Harayama. "''Alcanivorax'' Which Prevails In Oil-contaminated Seawater Exhibits Broad Substrate Specificity For Alkane Degradation." Environmental Microbiology 5.9 (2003): 746-753.]<br />
<br />
[3] [http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html Lorenzo, Víctor De. "Blueprint of an Oil-eating Bacterium." Nature Biotechnology 24 (2006): 952-953.]<br />
<br />
[4] [http://www.ncbi.nlm.nih.gov/pubmed/16878126 Schneiker, S. et al. "Genome Sequence of the Ubiquitous Hydrocarbon-degrading Marine Bacterium A''lcanivorax Borkumensis''." Nature Biotechnology 24 (2006): 997-1004.]<br />
<br />
[5] [http://ijs.sgmjournals.org/cgi/content/abstract/48/2/339 Yakimov, Michail M., Peter N. Golyshin, Siegmund Lang, Edward R. B. Moore, Wolf-Rainer Abraham, Heinrich Lunsdorf, and Kenneth N. Timmis. "''Alcanivorax Borkumensis'' gen. nov., sp. nov., A New, Hydrocarbon-degrading And Surfactant-producing Marine Bacterium." International Journal of Systematic Bacteriology 48 (1998): 339-348.]<br />
<br />
==Author==<br />
Page authored by Andrew Buss, student of [http://www.kbs.msu.edu/faculty/lennon/ Prof. Jay Lennon] at Michigan State University.</div>Bussand1https://microbewiki.kenyon.edu/index.php?title=Alcanivorax&diff=29754Alcanivorax2008-04-06T20:49:51Z<p>Bussand1: /* Cell Structure, Metabolism and Life Cycle */</p>
<hr />
<div>[[Image:AlcVorax PROCARYOTES01b-1.JPG|thumbnail|300px|Figure 1. ''Alcanivorax borkumensis''. Image from Helmholtz Centre for Infection Research[http://www.helmholtz-hzi.de/en/news_public_relation/press_releases/view/article/complete/oil_tanker_accidents_as_a_source_of_food/]]]<br />
<br />
==Classification==<br />
<br />
'''Bacteria'''; Phylum: '''Proteobacteria'''; Class: '''Gammaproteobacteria'''; Order: '''Oceanospirillales'''; Family: '''Alcanivoracaceae'''<br />
<br />
===Species===<br />
<br />
{|<br />
| height="10" bgcolor="#FFDF95" |<br />
'''NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Tree&id=2&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy]'''<br />
|}<br />
<br />
*''Alcanivorax balearicum''<br />
*''Alcanivorax borkumensis''<br />
*''Alcanivorax dieselolei''<br />
*''Alcanivorax indicus''<br />
*''Alcanivorax jadensis''<br />
*''Alcanivorax venustensis''<br />
<br />
==Description and Significance==<br />
[[Image:PWS tanker oil spill.jpg|thumbnail|200px|Figure 2. Supertanker Exxon Valdez grounded on Bligh Reef which released 11 million gallons of crude oil into the water. This oil-contaminated seawater is the preferred habitat for ''Alcanivorax''. Image from USGS[http://menlocampus.wr.usgs.gov/50years/accomplishments/oil.html]]]<br />
<br />
''Alcanivorax'', first described in 1998, is a Gram-negative, halophilic, aerobic, rod-shaped, oil-degrading marine bacterium that is found in low abundances in unpolluted environments in the upper layers of the ocean, but quickly becomes the predominant microbe in oil-contaminated open oceans and coastal waters when nitrogen and phosphorus are not limiting [2]. When conditions in these moderately halophilic environments are right, ''Alcanivorax'' may make up 80-90% of the oil-degrading microbes present in the area [4]. It is described as a non-motile bactertium which is true for species such as ''Alcanivorax borkumensis'', but other species such as ''Alcanivorax venustensis'' were described to be motile by polar flagella [1]. The optimial conditions described for A.borkumensis growth include temperatures in the range of 20-30 degrees C, and a NaCl concentration of 3-10%. Cells grown with pyruvate were observed to be 2.0-3.0um in length and 0.4-07um in diameter, however, cells were shorter (1.0-1.5um in length) when cells were grown with n-alkanes [5].<br />
<br />
As a result of their profound ability to degrade and live predominately on alkanes, as well as to become the dominant microbes in oil-contaminated areas, ''Alcanivorax'' plays a huge role in the biological cleanup of oil-contaminated environments. These oil-contaminated environments in the ocean are largely due to anthropogenic sources such as oil spills caused by tankers accidents, and cause serious ecological damage to plants and animals on the coast as well as other inhabitants of the ocean. Microbes such as ''Alcanivorax'' provide a major route for the breakdown of these pollutants, and demonstrate how marine bacteria keep the environment in check. Of all the ''Alcanivorax'' species and other oil-degrading microbes, ''Alcanivorax borkumensis'' is one of the most important worldwide due to the fact it produces a wide variety of very efficient oil-degrading enzymes. With this knowledge, ''A. borkumensis'' could provide a useful tool for bioremediation of oil spills.<br />
<br />
==Genome Structure==<br />
[[Image:Lorenzo.gif|thumbnail|200px|Figure 3. Mechanisms for oil degradation and survival encoded by the ''A. borkumensis SK2'' genome. Image from Victor de Lorenzo[http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html]]]<br />
<br />
The ''Alcanivorax borkumensis'' strain SK2, isolated from a seawater sediment sample in the North Sea at a site located near the Isle of Borkum, was the first hydrocarbonoclastic bacterium to be sequenced and was completed by Susanne Schneiker et al. It's genome consists of a single circular chromosome with 3,120,143 base pairs with an average G+C content of 54.7%. The genomic analysis of ''A. borkumensis SK2'' revealed several new insights into the bacterium's role for (i) n-alkane degradation (which includes metabolism, biosurfactant production and biofilm production), (ii) it's system for capturing or scavenging the small amounts of nitrogen, phosphorous, sulfur, and other elements in a nutrient-poor marine environment which allows for more efficient alkane degradation due to their main limitation of nutrient availability, (iii) as well as means for coping with stress factors such as high salt contents and high UV radiation since it thrives mostly in the upper layers in the ocean where UV light is encountered (Figure 3). It's genome encodes several systems for the catabolism of hydrocarbons which allow the bacertium to degrade all sorts of alkanes such as AlkB1 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C5-C12, and AlkB2 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C8 to C16. Both these systems are located close to the origin of replication of the chromosome. ''A. borkumensis'' is also able to degrade alkanes up to C32, branched aliphatic hydrocarbons, isoprenoid hydrocarbons such as phytane, as well as alkylarenes and alkylcycloalkanes. Thus, the genome encodes for a broad spectrum of systems for the catabolism of hydrocarbons, giving it a competitive advantage over other oil-degrading marine microbial communities. To deal with the damaging effects of UV light, ''A. borkumensis'' has a number of genes that reduce the damage. These include the full genes for DNA alkylation, recombinational and nucleotide excision repair, base excision repair, as well as the SOS response [4].<br />
<br />
==Cell Structure, Metabolism and Life Cycle==<br />
Alcanivorax borkumensis, a chemoorganotroph, is able to use n-alkanes as its principle carbon and energy source by use of the broad spectrum of oil-degrading enzymes it possesses, but they can also use a limited number of organic compounds such as aliphatic hydrocarbons, volatile fatty acids, and pyruvate. However, it cannot utilize carbon sources such as sugars or amino acids. When the slow growing ''A. borkumensis'' uses n-alkanes exclusively, the microbes produce extracellular and membrane-bound surface-active glucose lipids called biosurfactants. These biosurfactants reduce the surface tension of water from 72 to 29 dynes/cm and act as natural emulsifiers which enhances the break up of oil-in-water emulsions which prevent degradation of alkanes [4,5]. Due to the low solubility of oil in water, most oil degradation takes place at the oil-water interface where ''A. borkumensis'' attaches and forms a biofilm around the oil droplets as depicted in Figure 3.<br />
<br />
==Ecology and Pathogenesis==<br />
Habitat; symbiosis; biogeochemical significance; contributions to environment.<br><br />
If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.<br><br><br />
<br />
==References==<br />
[1] [http://ijs.sgmjournals.org/cgi/content/abstract/53/1/331 Fernandez-Martinez, Javier, Maria J. Pujalte, Jesus Garcia-Martinez, Manuel Mata, Esperanza Garay, and Francisco Rodriguez-Valera. "Description of ''Alcanivorax Venustensis'' sp. nov. and Reclassification of ''Fundibacter Jadensis'' DSM 12178T (Bruns and Berthe-Corti 1999) As ''Alcanivorax Jadensis'' comb. nov., Members of the Emended Genus ''Alcanivorax''." International Journal of Systematic and Evolutionary Microbiology 53 (2003): 331-338.]<br />
<br />
[2] [http://www.blackwell-synergy.com/doi/pdf/10.1046/j.1468-2920.2003.00468.x Hara, Akihiro, Kazuaki Syutsubo, and Shigeaki Harayama. "''Alcanivorax'' Which Prevails In Oil-contaminated Seawater Exhibits Broad Substrate Specificity For Alkane Degradation." Environmental Microbiology 5.9 (2003): 746-753.]<br />
<br />
[3] [http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html Lorenzo, Víctor De. "Blueprint of an Oil-eating Bacterium." Nature Biotechnology 24 (2006): 952-953.]<br />
<br />
[4] [http://www.ncbi.nlm.nih.gov/pubmed/16878126 Schneiker, S. et al. "Genome Sequence of the Ubiquitous Hydrocarbon-degrading Marine Bacterium A''lcanivorax Borkumensis''." Nature Biotechnology 24 (2006): 997-1004.]<br />
<br />
[5] [http://ijs.sgmjournals.org/cgi/content/abstract/48/2/339 Yakimov, Michail M., Peter N. Golyshin, Siegmund Lang, Edward R. B. Moore, Wolf-Rainer Abraham, Heinrich Lunsdorf, and Kenneth N. Timmis. "''Alcanivorax Borkumensis'' gen. nov., sp. nov., A New, Hydrocarbon-degrading And Surfactant-producing Marine Bacterium." International Journal of Systematic Bacteriology 48 (1998): 339-348.]<br />
<br />
==Author==<br />
Page authored by Andrew Buss, student of [http://www.kbs.msu.edu/faculty/lennon/ Prof. Jay Lennon] at Michigan State University.</div>Bussand1https://microbewiki.kenyon.edu/index.php?title=Alcanivorax&diff=29753Alcanivorax2008-04-06T20:49:33Z<p>Bussand1: /* Cell Structure, Metabolism and Life Cycle */</p>
<hr />
<div>[[Image:AlcVorax PROCARYOTES01b-1.JPG|thumbnail|300px|Figure 1. ''Alcanivorax borkumensis''. Image from Helmholtz Centre for Infection Research[http://www.helmholtz-hzi.de/en/news_public_relation/press_releases/view/article/complete/oil_tanker_accidents_as_a_source_of_food/]]]<br />
<br />
==Classification==<br />
<br />
'''Bacteria'''; Phylum: '''Proteobacteria'''; Class: '''Gammaproteobacteria'''; Order: '''Oceanospirillales'''; Family: '''Alcanivoracaceae'''<br />
<br />
===Species===<br />
<br />
{|<br />
| height="10" bgcolor="#FFDF95" |<br />
'''NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Tree&id=2&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy]'''<br />
|}<br />
<br />
*''Alcanivorax balearicum''<br />
*''Alcanivorax borkumensis''<br />
*''Alcanivorax dieselolei''<br />
*''Alcanivorax indicus''<br />
*''Alcanivorax jadensis''<br />
*''Alcanivorax venustensis''<br />
<br />
==Description and Significance==<br />
[[Image:PWS tanker oil spill.jpg|thumbnail|200px|Figure 2. Supertanker Exxon Valdez grounded on Bligh Reef which released 11 million gallons of crude oil into the water. This oil-contaminated seawater is the preferred habitat for ''Alcanivorax''. Image from USGS[http://menlocampus.wr.usgs.gov/50years/accomplishments/oil.html]]]<br />
<br />
''Alcanivorax'', first described in 1998, is a Gram-negative, halophilic, aerobic, rod-shaped, oil-degrading marine bacterium that is found in low abundances in unpolluted environments in the upper layers of the ocean, but quickly becomes the predominant microbe in oil-contaminated open oceans and coastal waters when nitrogen and phosphorus are not limiting [2]. When conditions in these moderately halophilic environments are right, ''Alcanivorax'' may make up 80-90% of the oil-degrading microbes present in the area [4]. It is described as a non-motile bactertium which is true for species such as ''Alcanivorax borkumensis'', but other species such as ''Alcanivorax venustensis'' were described to be motile by polar flagella [1]. The optimial conditions described for A.borkumensis growth include temperatures in the range of 20-30 degrees C, and a NaCl concentration of 3-10%. Cells grown with pyruvate were observed to be 2.0-3.0um in length and 0.4-07um in diameter, however, cells were shorter (1.0-1.5um in length) when cells were grown with n-alkanes [5].<br />
<br />
As a result of their profound ability to degrade and live predominately on alkanes, as well as to become the dominant microbes in oil-contaminated areas, ''Alcanivorax'' plays a huge role in the biological cleanup of oil-contaminated environments. These oil-contaminated environments in the ocean are largely due to anthropogenic sources such as oil spills caused by tankers accidents, and cause serious ecological damage to plants and animals on the coast as well as other inhabitants of the ocean. Microbes such as ''Alcanivorax'' provide a major route for the breakdown of these pollutants, and demonstrate how marine bacteria keep the environment in check. Of all the ''Alcanivorax'' species and other oil-degrading microbes, ''Alcanivorax borkumensis'' is one of the most important worldwide due to the fact it produces a wide variety of very efficient oil-degrading enzymes. With this knowledge, ''A. borkumensis'' could provide a useful tool for bioremediation of oil spills.<br />
<br />
==Genome Structure==<br />
[[Image:Lorenzo.gif|thumbnail|200px|Figure 3. Mechanisms for oil degradation and survival encoded by the ''A. borkumensis SK2'' genome. Image from Victor de Lorenzo[http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html]]]<br />
<br />
The ''Alcanivorax borkumensis'' strain SK2, isolated from a seawater sediment sample in the North Sea at a site located near the Isle of Borkum, was the first hydrocarbonoclastic bacterium to be sequenced and was completed by Susanne Schneiker et al. It's genome consists of a single circular chromosome with 3,120,143 base pairs with an average G+C content of 54.7%. The genomic analysis of ''A. borkumensis SK2'' revealed several new insights into the bacterium's role for (i) n-alkane degradation (which includes metabolism, biosurfactant production and biofilm production), (ii) it's system for capturing or scavenging the small amounts of nitrogen, phosphorous, sulfur, and other elements in a nutrient-poor marine environment which allows for more efficient alkane degradation due to their main limitation of nutrient availability, (iii) as well as means for coping with stress factors such as high salt contents and high UV radiation since it thrives mostly in the upper layers in the ocean where UV light is encountered (Figure 3). It's genome encodes several systems for the catabolism of hydrocarbons which allow the bacertium to degrade all sorts of alkanes such as AlkB1 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C5-C12, and AlkB2 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C8 to C16. Both these systems are located close to the origin of replication of the chromosome. ''A. borkumensis'' is also able to degrade alkanes up to C32, branched aliphatic hydrocarbons, isoprenoid hydrocarbons such as phytane, as well as alkylarenes and alkylcycloalkanes. Thus, the genome encodes for a broad spectrum of systems for the catabolism of hydrocarbons, giving it a competitive advantage over other oil-degrading marine microbial communities. To deal with the damaging effects of UV light, ''A. borkumensis'' has a number of genes that reduce the damage. These include the full genes for DNA alkylation, recombinational and nucleotide excision repair, base excision repair, as well as the SOS response [4].<br />
<br />
==Cell Structure, Metabolism and Life Cycle==<br />
Alcanivorax borkumensis, a chemoorganotroph, is able to use n-alkanes as its principle carbon and energy source by use of the broad spectrum of oil-degrading enzymes it possesses, but they can also use a limited number of organic compounds such as aliphatic hydrocarbons, volatile fatty acids, and pyruvate. However, it cannot utilize carbon sources such as sugars or amino acids. When the slow growing ''A. borkumensis'' uses n-alkanes exclusively, the microbes produce extracellular and membrane-bound surface-active glucose lipids called biosurfactants. These biosurfactants reduce the surface tension of water from 72 to 29 dynes/cm and act as natural emulsifiers which enhances the break up of oil-in-water emulsions which prevent degradation of alkanes [4]. Due to the low solubility of oil in water, most oil degradation takes place at the oil-water interface where ''A. borkumensis'' attaches and forms a biofilm around the oil droplets as depicted in Figure 3.<br />
<br />
==Ecology and Pathogenesis==<br />
Habitat; symbiosis; biogeochemical significance; contributions to environment.<br><br />
If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.<br><br><br />
<br />
==References==<br />
[1] [http://ijs.sgmjournals.org/cgi/content/abstract/53/1/331 Fernandez-Martinez, Javier, Maria J. Pujalte, Jesus Garcia-Martinez, Manuel Mata, Esperanza Garay, and Francisco Rodriguez-Valera. "Description of ''Alcanivorax Venustensis'' sp. nov. and Reclassification of ''Fundibacter Jadensis'' DSM 12178T (Bruns and Berthe-Corti 1999) As ''Alcanivorax Jadensis'' comb. nov., Members of the Emended Genus ''Alcanivorax''." International Journal of Systematic and Evolutionary Microbiology 53 (2003): 331-338.]<br />
<br />
[2] [http://www.blackwell-synergy.com/doi/pdf/10.1046/j.1468-2920.2003.00468.x Hara, Akihiro, Kazuaki Syutsubo, and Shigeaki Harayama. "''Alcanivorax'' Which Prevails In Oil-contaminated Seawater Exhibits Broad Substrate Specificity For Alkane Degradation." Environmental Microbiology 5.9 (2003): 746-753.]<br />
<br />
[3] [http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html Lorenzo, Víctor De. "Blueprint of an Oil-eating Bacterium." Nature Biotechnology 24 (2006): 952-953.]<br />
<br />
[4] [http://www.ncbi.nlm.nih.gov/pubmed/16878126 Schneiker, S. et al. "Genome Sequence of the Ubiquitous Hydrocarbon-degrading Marine Bacterium A''lcanivorax Borkumensis''." Nature Biotechnology 24 (2006): 997-1004.]<br />
<br />
[5] [http://ijs.sgmjournals.org/cgi/content/abstract/48/2/339 Yakimov, Michail M., Peter N. Golyshin, Siegmund Lang, Edward R. B. Moore, Wolf-Rainer Abraham, Heinrich Lunsdorf, and Kenneth N. Timmis. "''Alcanivorax Borkumensis'' gen. nov., sp. nov., A New, Hydrocarbon-degrading And Surfactant-producing Marine Bacterium." International Journal of Systematic Bacteriology 48 (1998): 339-348.]<br />
<br />
==Author==<br />
Page authored by Andrew Buss, student of [http://www.kbs.msu.edu/faculty/lennon/ Prof. Jay Lennon] at Michigan State University.</div>Bussand1https://microbewiki.kenyon.edu/index.php?title=Alcanivorax&diff=29749Alcanivorax2008-04-06T20:40:25Z<p>Bussand1: /* Description and Significance */</p>
<hr />
<div>[[Image:AlcVorax PROCARYOTES01b-1.JPG|thumbnail|300px|Figure 1. ''Alcanivorax borkumensis''. Image from Helmholtz Centre for Infection Research[http://www.helmholtz-hzi.de/en/news_public_relation/press_releases/view/article/complete/oil_tanker_accidents_as_a_source_of_food/]]]<br />
<br />
==Classification==<br />
<br />
'''Bacteria'''; Phylum: '''Proteobacteria'''; Class: '''Gammaproteobacteria'''; Order: '''Oceanospirillales'''; Family: '''Alcanivoracaceae'''<br />
<br />
===Species===<br />
<br />
{|<br />
| height="10" bgcolor="#FFDF95" |<br />
'''NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Tree&id=2&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy]'''<br />
|}<br />
<br />
*''Alcanivorax balearicum''<br />
*''Alcanivorax borkumensis''<br />
*''Alcanivorax dieselolei''<br />
*''Alcanivorax indicus''<br />
*''Alcanivorax jadensis''<br />
*''Alcanivorax venustensis''<br />
<br />
==Description and Significance==<br />
[[Image:PWS tanker oil spill.jpg|thumbnail|200px|Figure 2. Supertanker Exxon Valdez grounded on Bligh Reef which released 11 million gallons of crude oil into the water. This oil-contaminated seawater is the preferred habitat for ''Alcanivorax''. Image from USGS[http://menlocampus.wr.usgs.gov/50years/accomplishments/oil.html]]]<br />
<br />
''Alcanivorax'', first described in 1998, is a Gram-negative, halophilic, aerobic, rod-shaped, oil-degrading marine bacterium that is found in low abundances in unpolluted environments in the upper layers of the ocean, but quickly becomes the predominant microbe in oil-contaminated open oceans and coastal waters when nitrogen and phosphorus are not limiting [2]. When conditions in these moderately halophilic environments are right, ''Alcanivorax'' may make up 80-90% of the oil-degrading microbes present in the area [4]. It is described as a non-motile bactertium which is true for species such as ''Alcanivorax borkumensis'', but other species such as ''Alcanivorax venustensis'' were described to be motile by polar flagella [1]. The optimial conditions described for A.borkumensis growth include temperatures in the range of 20-30 degrees C, and a NaCl concentration of 3-10%. Cells grown with pyruvate were observed to be 2.0-3.0um in length and 0.4-07um in diameter, however, cells were shorter (1.0-1.5um in length) when cells were grown with n-alkanes [5].<br />
<br />
As a result of their profound ability to degrade and live predominately on alkanes, as well as to become the dominant microbes in oil-contaminated areas, ''Alcanivorax'' plays a huge role in the biological cleanup of oil-contaminated environments. These oil-contaminated environments in the ocean are largely due to anthropogenic sources such as oil spills caused by tankers accidents, and cause serious ecological damage to plants and animals on the coast as well as other inhabitants of the ocean. Microbes such as ''Alcanivorax'' provide a major route for the breakdown of these pollutants, and demonstrate how marine bacteria keep the environment in check. Of all the ''Alcanivorax'' species and other oil-degrading microbes, ''Alcanivorax borkumensis'' is one of the most important worldwide due to the fact it produces a wide variety of very efficient oil-degrading enzymes. With this knowledge, ''A. borkumensis'' could provide a useful tool for bioremediation of oil spills.<br />
<br />
==Genome Structure==<br />
[[Image:Lorenzo.gif|thumbnail|200px|Figure 3. Mechanisms for oil degradation and survival encoded by the ''A. borkumensis SK2'' genome. Image from Victor de Lorenzo[http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html]]]<br />
<br />
The ''Alcanivorax borkumensis'' strain SK2, isolated from a seawater sediment sample in the North Sea at a site located near the Isle of Borkum, was the first hydrocarbonoclastic bacterium to be sequenced and was completed by Susanne Schneiker et al. It's genome consists of a single circular chromosome with 3,120,143 base pairs with an average G+C content of 54.7%. The genomic analysis of ''A. borkumensis SK2'' revealed several new insights into the bacterium's role for (i) n-alkane degradation (which includes metabolism, biosurfactant production and biofilm production), (ii) it's system for capturing or scavenging the small amounts of nitrogen, phosphorous, sulfur, and other elements in a nutrient-poor marine environment which allows for more efficient alkane degradation due to their main limitation of nutrient availability, (iii) as well as means for coping with stress factors such as high salt contents and high UV radiation since it thrives mostly in the upper layers in the ocean where UV light is encountered (Figure 3). It's genome encodes several systems for the catabolism of hydrocarbons which allow the bacertium to degrade all sorts of alkanes such as AlkB1 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C5-C12, and AlkB2 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C8 to C16. Both these systems are located close to the origin of replication of the chromosome. ''A. borkumensis'' is also able to degrade alkanes up to C32, branched aliphatic hydrocarbons, isoprenoid hydrocarbons such as phytane, as well as alkylarenes and alkylcycloalkanes. Thus, the genome encodes for a broad spectrum of systems for the catabolism of hydrocarbons, giving it a competitive advantage over other oil-degrading marine microbial communities. To deal with the damaging effects of UV light, ''A. borkumensis'' has a number of genes that reduce the damage. These include the full genes for DNA alkylation, recombinational and nucleotide excision repair, base excision repair, as well as the SOS response [4].<br />
<br />
==Cell Structure, Metabolism and Life Cycle==<br />
Alcanivorax borkumensis is able to use n-alkanes as its principle carbon and energy source by use of the broad spectrum of oil-degrading enzymes it possesses, but they can also use a limited number of organic compounds such as aliphatic hydrocarbons, volatile fatty acids, and pyruvate. However, it cannot utilize carbon sources such as sugars or amino acids. When the slow growing ''A. borkumensis'' uses n-alkanes exclusively, the microbes produce extracellular and membrane-bound surface-active glucose lipids called biosurfactants. These biosurfactants act as natural emulsifiers which enhances the break up of oil-in-water emulsions which prevent degradation of alkanes [4]. Due to the low solubility of oil in water, most oil degradation takes place at the oil-water interface where ''A. borkumensis'' attaches and forms a biofilm around the oil droplets as depicted in Figure 3.<br />
<br />
==Ecology and Pathogenesis==<br />
Habitat; symbiosis; biogeochemical significance; contributions to environment.<br><br />
If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.<br><br><br />
<br />
==References==<br />
[1] [http://ijs.sgmjournals.org/cgi/content/abstract/53/1/331 Fernandez-Martinez, Javier, Maria J. Pujalte, Jesus Garcia-Martinez, Manuel Mata, Esperanza Garay, and Francisco Rodriguez-Valera. "Description of ''Alcanivorax Venustensis'' sp. nov. and Reclassification of ''Fundibacter Jadensis'' DSM 12178T (Bruns and Berthe-Corti 1999) As ''Alcanivorax Jadensis'' comb. nov., Members of the Emended Genus ''Alcanivorax''." International Journal of Systematic and Evolutionary Microbiology 53 (2003): 331-338.]<br />
<br />
[2] [http://www.blackwell-synergy.com/doi/pdf/10.1046/j.1468-2920.2003.00468.x Hara, Akihiro, Kazuaki Syutsubo, and Shigeaki Harayama. "''Alcanivorax'' Which Prevails In Oil-contaminated Seawater Exhibits Broad Substrate Specificity For Alkane Degradation." Environmental Microbiology 5.9 (2003): 746-753.]<br />
<br />
[3] [http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html Lorenzo, Víctor De. "Blueprint of an Oil-eating Bacterium." Nature Biotechnology 24 (2006): 952-953.]<br />
<br />
[4] [http://www.ncbi.nlm.nih.gov/pubmed/16878126 Schneiker, S. et al. "Genome Sequence of the Ubiquitous Hydrocarbon-degrading Marine Bacterium A''lcanivorax Borkumensis''." Nature Biotechnology 24 (2006): 997-1004.]<br />
<br />
[5] [http://ijs.sgmjournals.org/cgi/content/abstract/48/2/339 Yakimov, Michail M., Peter N. Golyshin, Siegmund Lang, Edward R. B. Moore, Wolf-Rainer Abraham, Heinrich Lunsdorf, and Kenneth N. Timmis. "''Alcanivorax Borkumensis'' gen. nov., sp. nov., A New, Hydrocarbon-degrading And Surfactant-producing Marine Bacterium." International Journal of Systematic Bacteriology 48 (1998): 339-348.]<br />
<br />
==Author==<br />
Page authored by Andrew Buss, student of [http://www.kbs.msu.edu/faculty/lennon/ Prof. Jay Lennon] at Michigan State University.</div>Bussand1https://microbewiki.kenyon.edu/index.php?title=Alcanivorax&diff=29748Alcanivorax2008-04-06T20:35:43Z<p>Bussand1: /* Description and Significance */</p>
<hr />
<div>[[Image:AlcVorax PROCARYOTES01b-1.JPG|thumbnail|300px|Figure 1. ''Alcanivorax borkumensis''. Image from Helmholtz Centre for Infection Research[http://www.helmholtz-hzi.de/en/news_public_relation/press_releases/view/article/complete/oil_tanker_accidents_as_a_source_of_food/]]]<br />
<br />
==Classification==<br />
<br />
'''Bacteria'''; Phylum: '''Proteobacteria'''; Class: '''Gammaproteobacteria'''; Order: '''Oceanospirillales'''; Family: '''Alcanivoracaceae'''<br />
<br />
===Species===<br />
<br />
{|<br />
| height="10" bgcolor="#FFDF95" |<br />
'''NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Tree&id=2&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy]'''<br />
|}<br />
<br />
*''Alcanivorax balearicum''<br />
*''Alcanivorax borkumensis''<br />
*''Alcanivorax dieselolei''<br />
*''Alcanivorax indicus''<br />
*''Alcanivorax jadensis''<br />
*''Alcanivorax venustensis''<br />
<br />
==Description and Significance==<br />
[[Image:PWS tanker oil spill.jpg|thumbnail|200px|Figure 2. Supertanker Exxon Valdez grounded on Bligh Reef which released 11 million gallons of crude oil into the water. This oil-contaminated seawater is the preferred habitat for ''Alcanivorax''. Image from USGS[http://menlocampus.wr.usgs.gov/50years/accomplishments/oil.html]]]<br />
<br />
''Alcanivorax'', first described in 1998, is a Gram-negative, halophilic, aerobic, rod-shaped, oil-degrading marine bacterium that is found in low abundances in unpolluted environments in the upper layers of the ocean, but quickly becomes the predominant microbe in oil-contaminated open oceans and coastal waters when nitrogen and phosphorus are not limiting [2]. When conditions in these moderately halophilic environments are right, ''Alcanivorax'' may make up 80-90% of the oil-degrading microbes present in the area [4]. It is described as a non-motile bactertium which is true for species such as ''Alcanivorax borkumensis'', but other species such as ''Alcanivorax venustensis'' were described to be motile by polar flagella [1]. The optimial conditions described for A.borkumensis growth include temperatures in the range of 20-30 degrees C, and a NaCl concentration of 3-10%. Cells grown with pyruvate were observed to be 2.0-3.0um in length and 0.4-07um in diameter, however, cells were shorter (1.0-1.5um in length) when cells were grown with n-alkanes [5].<br />
As a result of their profound ability to degrade and live predominately on alkanes, as well as to become the dominant microbes in oil-contaminated areas, ''Alcanivorax'' plays a huge role in the biological cleanup of oil-contaminated environments. These oil-contaminated environments in the ocean are largely due to anthropogenic sources such as oil spills caused by tankers accidents, and cause serious ecological damage to plants and animals on the coast as well as other inhabitants of the ocean. Microbes such as ''Alcanivorax'' provide a major route for the breakdown of these pollutants, and demonstrate how marine bacteria keep the environment in check. Of all the ''Alcanivorax'' species and other oil-degrading microbes, ''Alcanivorax borkumensis'' is one of the most important worldwide due to the fact it produces a wide variety of very efficient oil-degrading enzymes. With this knowledge, ''A. borkumensis'' could provide a useful tool for bioremediation of oil spills.<br />
<br />
==Genome Structure==<br />
[[Image:Lorenzo.gif|thumbnail|200px|Figure 3. Mechanisms for oil degradation and survival encoded by the ''A. borkumensis SK2'' genome. Image from Victor de Lorenzo[http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html]]]<br />
<br />
The ''Alcanivorax borkumensis'' strain SK2, isolated from a seawater sediment sample in the North Sea at a site located near the Isle of Borkum, was the first hydrocarbonoclastic bacterium to be sequenced and was completed by Susanne Schneiker et al. It's genome consists of a single circular chromosome with 3,120,143 base pairs with an average G+C content of 54.7%. The genomic analysis of ''A. borkumensis SK2'' revealed several new insights into the bacterium's role for (i) n-alkane degradation (which includes metabolism, biosurfactant production and biofilm production), (ii) it's system for capturing or scavenging the small amounts of nitrogen, phosphorous, sulfur, and other elements in a nutrient-poor marine environment which allows for more efficient alkane degradation due to their main limitation of nutrient availability, (iii) as well as means for coping with stress factors such as high salt contents and high UV radiation since it thrives mostly in the upper layers in the ocean where UV light is encountered (Figure 3). It's genome encodes several systems for the catabolism of hydrocarbons which allow the bacertium to degrade all sorts of alkanes such as AlkB1 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C5-C12, and AlkB2 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C8 to C16. Both these systems are located close to the origin of replication of the chromosome. ''A. borkumensis'' is also able to degrade alkanes up to C32, branched aliphatic hydrocarbons, isoprenoid hydrocarbons such as phytane, as well as alkylarenes and alkylcycloalkanes. Thus, the genome encodes for a broad spectrum of systems for the catabolism of hydrocarbons, giving it a competitive advantage over other oil-degrading marine microbial communities. To deal with the damaging effects of UV light, ''A. borkumensis'' has a number of genes that reduce the damage. These include the full genes for DNA alkylation, recombinational and nucleotide excision repair, base excision repair, as well as the SOS response [4].<br />
<br />
==Cell Structure, Metabolism and Life Cycle==<br />
Alcanivorax borkumensis is able to use n-alkanes as its principle carbon and energy source by use of the broad spectrum of oil-degrading enzymes it possesses, but they can also use a limited number of organic compounds such as aliphatic hydrocarbons, volatile fatty acids, and pyruvate. However, it cannot utilize carbon sources such as sugars or amino acids. When the slow growing ''A. borkumensis'' uses n-alkanes exclusively, the microbes produce extracellular and membrane-bound surface-active glucose lipids called biosurfactants. These biosurfactants act as natural emulsifiers which enhances the break up of oil-in-water emulsions which prevent degradation of alkanes [4]. Due to the low solubility of oil in water, most oil degradation takes place at the oil-water interface where ''A. borkumensis'' attaches and forms a biofilm around the oil droplets as depicted in Figure 3.<br />
<br />
==Ecology and Pathogenesis==<br />
Habitat; symbiosis; biogeochemical significance; contributions to environment.<br><br />
If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.<br><br><br />
<br />
==References==<br />
[1] [http://ijs.sgmjournals.org/cgi/content/abstract/53/1/331 Fernandez-Martinez, Javier, Maria J. Pujalte, Jesus Garcia-Martinez, Manuel Mata, Esperanza Garay, and Francisco Rodriguez-Valera. "Description of ''Alcanivorax Venustensis'' sp. nov. and Reclassification of ''Fundibacter Jadensis'' DSM 12178T (Bruns and Berthe-Corti 1999) As ''Alcanivorax Jadensis'' comb. nov., Members of the Emended Genus ''Alcanivorax''." International Journal of Systematic and Evolutionary Microbiology 53 (2003): 331-338.]<br />
<br />
[2] [http://www.blackwell-synergy.com/doi/pdf/10.1046/j.1468-2920.2003.00468.x Hara, Akihiro, Kazuaki Syutsubo, and Shigeaki Harayama. "''Alcanivorax'' Which Prevails In Oil-contaminated Seawater Exhibits Broad Substrate Specificity For Alkane Degradation." Environmental Microbiology 5.9 (2003): 746-753.]<br />
<br />
[3] [http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html Lorenzo, Víctor De. "Blueprint of an Oil-eating Bacterium." Nature Biotechnology 24 (2006): 952-953.]<br />
<br />
[4] [http://www.ncbi.nlm.nih.gov/pubmed/16878126 Schneiker, S. et al. "Genome Sequence of the Ubiquitous Hydrocarbon-degrading Marine Bacterium A''lcanivorax Borkumensis''." Nature Biotechnology 24 (2006): 997-1004.]<br />
<br />
[5] [http://ijs.sgmjournals.org/cgi/content/abstract/48/2/339 Yakimov, Michail M., Peter N. Golyshin, Siegmund Lang, Edward R. B. Moore, Wolf-Rainer Abraham, Heinrich Lunsdorf, and Kenneth N. Timmis. "''Alcanivorax Borkumensis'' gen. nov., sp. nov., A New, Hydrocarbon-degrading And Surfactant-producing Marine Bacterium." International Journal of Systematic Bacteriology 48 (1998): 339-348.]<br />
<br />
==Author==<br />
Page authored by Andrew Buss, student of [http://www.kbs.msu.edu/faculty/lennon/ Prof. Jay Lennon] at Michigan State University.</div>Bussand1https://microbewiki.kenyon.edu/index.php?title=Alcanivorax&diff=29747Alcanivorax2008-04-06T20:15:30Z<p>Bussand1: /* Genome Structure */</p>
<hr />
<div>[[Image:AlcVorax PROCARYOTES01b-1.JPG|thumbnail|300px|Figure 1. ''Alcanivorax borkumensis''. Image from Helmholtz Centre for Infection Research[http://www.helmholtz-hzi.de/en/news_public_relation/press_releases/view/article/complete/oil_tanker_accidents_as_a_source_of_food/]]]<br />
<br />
==Classification==<br />
<br />
'''Bacteria'''; Phylum: '''Proteobacteria'''; Class: '''Gammaproteobacteria'''; Order: '''Oceanospirillales'''; Family: '''Alcanivoracaceae'''<br />
<br />
===Species===<br />
<br />
{|<br />
| height="10" bgcolor="#FFDF95" |<br />
'''NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Tree&id=2&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy]'''<br />
|}<br />
<br />
*''Alcanivorax balearicum''<br />
*''Alcanivorax borkumensis''<br />
*''Alcanivorax dieselolei''<br />
*''Alcanivorax indicus''<br />
*''Alcanivorax jadensis''<br />
*''Alcanivorax venustensis''<br />
<br />
==Description and Significance==<br />
[[Image:PWS tanker oil spill.jpg|thumbnail|200px|Figure 2. Supertanker Exxon Valdez grounded on Bligh Reef which released 11 million gallons of crude oil into the water. This oil-contaminated seawater is the preferred habitat for ''Alcanivorax''. Image from USGS[http://menlocampus.wr.usgs.gov/50years/accomplishments/oil.html]]]<br />
<br />
''Alcanivorax'', first described in 1998, is a Gram-negative, halophilic, aerobic, rod-shaped, oil-degrading marine bacterium that is found in low abundances in unpolluted environments in the upper layers of the ocean, but quickly becomes the predominant microbe in oil-contaminated open oceans and coastal waters when nitrogen and phosphorus are not limiting [2]. When conditions in these moderately halophilic environments are right, ''Alcanivorax'' may make up 80-90% of the oil-degrading microbes present in the area [4]. It is described as a non-motile bactertium which is true for species such as ''Alcanivorax borkumensis'', but other species such as ''Alcanivorax venustensis'' were described to be motile by polar flagella [1]. <br />
<br />
As a result of their profound ability to degrade and live predominately on alkanes, as well as to become the dominant microbes in oil-contaminated areas, ''Alcanivorax'' plays a huge role in the biological cleanup of oil-contaminated environments. These oil-contaminated environments in the ocean are largely due to anthropogenic sources such as oil spills caused by tankers accidents, and cause serious ecological damage to coastal fauna and flora as well as other inhabitants of the ocean. Microbes such as ''Alcanivorax'' provide a major route for the breakdown of these pollutants, and demonstrate how marine bacteria keep the environment in check. Of all the ''Alcanivorax'' species and other oil-degrading microbes, ''Alcanivorax borkumensis'' is one of the most important worldwide due to the fact it produces a wide variety of very efficient oil-degrading enzymes. With this knowledge, ''A. borkumensis'' could provide a useful tool for bioremediation of oil spills.<br />
<br />
==Genome Structure==<br />
[[Image:Lorenzo.gif|thumbnail|200px|Figure 3. Mechanisms for oil degradation and survival encoded by the ''A. borkumensis SK2'' genome. Image from Victor de Lorenzo[http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html]]]<br />
<br />
The ''Alcanivorax borkumensis'' strain SK2, isolated from a seawater sediment sample in the North Sea at a site located near the Isle of Borkum, was the first hydrocarbonoclastic bacterium to be sequenced and was completed by Susanne Schneiker et al. It's genome consists of a single circular chromosome with 3,120,143 base pairs with an average G+C content of 54.7%. The genomic analysis of ''A. borkumensis SK2'' revealed several new insights into the bacterium's role for (i) n-alkane degradation (which includes metabolism, biosurfactant production and biofilm production), (ii) it's system for capturing or scavenging the small amounts of nitrogen, phosphorous, sulfur, and other elements in a nutrient-poor marine environment which allows for more efficient alkane degradation due to their main limitation of nutrient availability, (iii) as well as means for coping with stress factors such as high salt contents and high UV radiation since it thrives mostly in the upper layers in the ocean where UV light is encountered (Figure 3). It's genome encodes several systems for the catabolism of hydrocarbons which allow the bacertium to degrade all sorts of alkanes such as AlkB1 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C5-C12, and AlkB2 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C8 to C16. Both these systems are located close to the origin of replication of the chromosome. ''A. borkumensis'' is also able to degrade alkanes up to C32, branched aliphatic hydrocarbons, isoprenoid hydrocarbons such as phytane, as well as alkylarenes and alkylcycloalkanes. Thus, the genome encodes for a broad spectrum of systems for the catabolism of hydrocarbons, giving it a competitive advantage over other oil-degrading marine microbial communities. To deal with the damaging effects of UV light, ''A. borkumensis'' has a number of genes that reduce the damage. These include the full genes for DNA alkylation, recombinational and nucleotide excision repair, base excision repair, as well as the SOS response [4].<br />
<br />
==Cell Structure, Metabolism and Life Cycle==<br />
Alcanivorax borkumensis is able to use n-alkanes as its principle carbon and energy source by use of the broad spectrum of oil-degrading enzymes it possesses, but they can also use a limited number of organic compounds such as aliphatic hydrocarbons, volatile fatty acids, and pyruvate. However, it cannot utilize carbon sources such as sugars or amino acids. When the slow growing ''A. borkumensis'' uses n-alkanes exclusively, the microbes produce extracellular and membrane-bound surface-active glucose lipids called biosurfactants. These biosurfactants act as natural emulsifiers which enhances the break up of oil-in-water emulsions which prevent degradation of alkanes [4]. Due to the low solubility of oil in water, most oil degradation takes place at the oil-water interface where ''A. borkumensis'' attaches and forms a biofilm around the oil droplets as depicted in Figure 3.<br />
<br />
==Ecology and Pathogenesis==<br />
Habitat; symbiosis; biogeochemical significance; contributions to environment.<br><br />
If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.<br><br><br />
<br />
==References==<br />
[1] [http://ijs.sgmjournals.org/cgi/content/abstract/53/1/331 Fernandez-Martinez, Javier, Maria J. Pujalte, Jesus Garcia-Martinez, Manuel Mata, Esperanza Garay, and Francisco Rodriguez-Valera. "Description of ''Alcanivorax Venustensis'' sp. nov. and Reclassification of ''Fundibacter Jadensis'' DSM 12178T (Bruns and Berthe-Corti 1999) As ''Alcanivorax Jadensis'' comb. nov., Members of the Emended Genus ''Alcanivorax''." International Journal of Systematic and Evolutionary Microbiology 53 (2003): 331-338.]<br />
<br />
[2] [http://www.blackwell-synergy.com/doi/pdf/10.1046/j.1468-2920.2003.00468.x Hara, Akihiro, Kazuaki Syutsubo, and Shigeaki Harayama. "''Alcanivorax'' Which Prevails In Oil-contaminated Seawater Exhibits Broad Substrate Specificity For Alkane Degradation." Environmental Microbiology 5.9 (2003): 746-753.]<br />
<br />
[3] [http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html Lorenzo, Víctor De. "Blueprint of an Oil-eating Bacterium." Nature Biotechnology 24 (2006): 952-953.]<br />
<br />
[4] [http://www.ncbi.nlm.nih.gov/pubmed/16878126 Schneiker, S. et al. "Genome Sequence of the Ubiquitous Hydrocarbon-degrading Marine Bacterium A''lcanivorax Borkumensis''." Nature Biotechnology 24 (2006): 997-1004.]<br />
<br />
[5] [http://ijs.sgmjournals.org/cgi/content/abstract/48/2/339 Yakimov, Michail M., Peter N. Golyshin, Siegmund Lang, Edward R. B. Moore, Wolf-Rainer Abraham, Heinrich Lunsdorf, and Kenneth N. Timmis. "''Alcanivorax Borkumensis'' gen. nov., sp. nov., A New, Hydrocarbon-degrading And Surfactant-producing Marine Bacterium." International Journal of Systematic Bacteriology 48 (1998): 339-348.]<br />
<br />
==Author==<br />
Page authored by Andrew Buss, student of [http://www.kbs.msu.edu/faculty/lennon/ Prof. Jay Lennon] at Michigan State University.</div>Bussand1https://microbewiki.kenyon.edu/index.php?title=Alcanivorax&diff=29746Alcanivorax2008-04-06T20:14:55Z<p>Bussand1: /* Cell Structure, Metabolism and Life Cycle */</p>
<hr />
<div>[[Image:AlcVorax PROCARYOTES01b-1.JPG|thumbnail|300px|Figure 1. ''Alcanivorax borkumensis''. Image from Helmholtz Centre for Infection Research[http://www.helmholtz-hzi.de/en/news_public_relation/press_releases/view/article/complete/oil_tanker_accidents_as_a_source_of_food/]]]<br />
<br />
==Classification==<br />
<br />
'''Bacteria'''; Phylum: '''Proteobacteria'''; Class: '''Gammaproteobacteria'''; Order: '''Oceanospirillales'''; Family: '''Alcanivoracaceae'''<br />
<br />
===Species===<br />
<br />
{|<br />
| height="10" bgcolor="#FFDF95" |<br />
'''NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Tree&id=2&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy]'''<br />
|}<br />
<br />
*''Alcanivorax balearicum''<br />
*''Alcanivorax borkumensis''<br />
*''Alcanivorax dieselolei''<br />
*''Alcanivorax indicus''<br />
*''Alcanivorax jadensis''<br />
*''Alcanivorax venustensis''<br />
<br />
==Description and Significance==<br />
[[Image:PWS tanker oil spill.jpg|thumbnail|200px|Figure 2. Supertanker Exxon Valdez grounded on Bligh Reef which released 11 million gallons of crude oil into the water. This oil-contaminated seawater is the preferred habitat for ''Alcanivorax''. Image from USGS[http://menlocampus.wr.usgs.gov/50years/accomplishments/oil.html]]]<br />
<br />
''Alcanivorax'', first described in 1998, is a Gram-negative, halophilic, aerobic, rod-shaped, oil-degrading marine bacterium that is found in low abundances in unpolluted environments in the upper layers of the ocean, but quickly becomes the predominant microbe in oil-contaminated open oceans and coastal waters when nitrogen and phosphorus are not limiting [2]. When conditions in these moderately halophilic environments are right, ''Alcanivorax'' may make up 80-90% of the oil-degrading microbes present in the area [4]. It is described as a non-motile bactertium which is true for species such as ''Alcanivorax borkumensis'', but other species such as ''Alcanivorax venustensis'' were described to be motile by polar flagella [1]. <br />
<br />
As a result of their profound ability to degrade and live predominately on alkanes, as well as to become the dominant microbes in oil-contaminated areas, ''Alcanivorax'' plays a huge role in the biological cleanup of oil-contaminated environments. These oil-contaminated environments in the ocean are largely due to anthropogenic sources such as oil spills caused by tankers accidents, and cause serious ecological damage to coastal fauna and flora as well as other inhabitants of the ocean. Microbes such as ''Alcanivorax'' provide a major route for the breakdown of these pollutants, and demonstrate how marine bacteria keep the environment in check. Of all the ''Alcanivorax'' species and other oil-degrading microbes, ''Alcanivorax borkumensis'' is one of the most important worldwide due to the fact it produces a wide variety of very efficient oil-degrading enzymes. With this knowledge, ''A. borkumensis'' could provide a useful tool for bioremediation of oil spills.<br />
<br />
==Genome Structure==<br />
[[Image:Lorenzo.gif|thumbnail|200px|Figure 3. Mechanisms for oil degradation and survival encoded by the ''A. borkumensis SK2'' genome. Image from Victor de Lorenzo[http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html]]]<br />
<br />
The ''Alcanivorax borkumensis'' strain SK2, isolated from a seawater sediment sample in the North Sea at a site located near the Isle of Borkum, was the first hydrocarbonoclastic bacterium to be sequenced and was completed by Susanne Schneiker et al. It's genome consists of a single circular chromosome with 3,120,143 base pairs with an average G+C content of 54.7%. The genomic analysis of ''A. borkumensis SK2'' revealed several new insights into the bacterium's role for (i) n-alkane degradation (which includes metabolism, biosurfactant production and biofilm production), (ii) it's system for capturing or scavenging the small amounts of nitrogen, phosphorous, sulfur, and other elements in a nutrient-poor marine environment which allows for more efficient alkane degradation due to their main limitation of nutrient availability, (iii) as well as means for coping with stress factors such as high salt contents and high UV radiation since it thrives mostly in the upper layers in the ocean where UV light is encountered. It's genome encodes several systems for the catabolism of hydrocarbons which allow the bacertium to degrade all sorts of alkanes such as AlkB1 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C5-C12, and AlkB2 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C8 to C16. Both these systems are located close to the origin of replication of the chromosome. ''A. borkumensis'' is also able to degrade alkanes up to C32, branched aliphatic hydrocarbons, isoprenoid hydrocarbons such as phytane, as well as alkylarenes and alkylcycloalkanes. Thus, the genome encodes for a broad spectrum of systems for the catabolism of hydrocarbons, giving it a competitive advantage over other oil-degrading marine microbial communities. To deal with the damaging effects of UV light, ''A. borkumensis'' has a number of genes that reduce the damage. These include the full genes for DNA alkylation, recombinational and nucleotide excision repair, base excision repair, as well as the SOS response [4].<br />
<br />
==Cell Structure, Metabolism and Life Cycle==<br />
Alcanivorax borkumensis is able to use n-alkanes as its principle carbon and energy source by use of the broad spectrum of oil-degrading enzymes it possesses, but they can also use a limited number of organic compounds such as aliphatic hydrocarbons, volatile fatty acids, and pyruvate. However, it cannot utilize carbon sources such as sugars or amino acids. When the slow growing ''A. borkumensis'' uses n-alkanes exclusively, the microbes produce extracellular and membrane-bound surface-active glucose lipids called biosurfactants. These biosurfactants act as natural emulsifiers which enhances the break up of oil-in-water emulsions which prevent degradation of alkanes [4]. Due to the low solubility of oil in water, most oil degradation takes place at the oil-water interface where ''A. borkumensis'' attaches and forms a biofilm around the oil droplets as depicted in Figure 3.<br />
<br />
==Ecology and Pathogenesis==<br />
Habitat; symbiosis; biogeochemical significance; contributions to environment.<br><br />
If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.<br><br><br />
<br />
==References==<br />
[1] [http://ijs.sgmjournals.org/cgi/content/abstract/53/1/331 Fernandez-Martinez, Javier, Maria J. Pujalte, Jesus Garcia-Martinez, Manuel Mata, Esperanza Garay, and Francisco Rodriguez-Valera. "Description of ''Alcanivorax Venustensis'' sp. nov. and Reclassification of ''Fundibacter Jadensis'' DSM 12178T (Bruns and Berthe-Corti 1999) As ''Alcanivorax Jadensis'' comb. nov., Members of the Emended Genus ''Alcanivorax''." International Journal of Systematic and Evolutionary Microbiology 53 (2003): 331-338.]<br />
<br />
[2] [http://www.blackwell-synergy.com/doi/pdf/10.1046/j.1468-2920.2003.00468.x Hara, Akihiro, Kazuaki Syutsubo, and Shigeaki Harayama. "''Alcanivorax'' Which Prevails In Oil-contaminated Seawater Exhibits Broad Substrate Specificity For Alkane Degradation." Environmental Microbiology 5.9 (2003): 746-753.]<br />
<br />
[3] [http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html Lorenzo, Víctor De. "Blueprint of an Oil-eating Bacterium." Nature Biotechnology 24 (2006): 952-953.]<br />
<br />
[4] [http://www.ncbi.nlm.nih.gov/pubmed/16878126 Schneiker, S. et al. "Genome Sequence of the Ubiquitous Hydrocarbon-degrading Marine Bacterium A''lcanivorax Borkumensis''." Nature Biotechnology 24 (2006): 997-1004.]<br />
<br />
[5] [http://ijs.sgmjournals.org/cgi/content/abstract/48/2/339 Yakimov, Michail M., Peter N. Golyshin, Siegmund Lang, Edward R. B. Moore, Wolf-Rainer Abraham, Heinrich Lunsdorf, and Kenneth N. Timmis. "''Alcanivorax Borkumensis'' gen. nov., sp. nov., A New, Hydrocarbon-degrading And Surfactant-producing Marine Bacterium." International Journal of Systematic Bacteriology 48 (1998): 339-348.]<br />
<br />
==Author==<br />
Page authored by Andrew Buss, student of [http://www.kbs.msu.edu/faculty/lennon/ Prof. Jay Lennon] at Michigan State University.</div>Bussand1https://microbewiki.kenyon.edu/index.php?title=Alcanivorax&diff=29745Alcanivorax2008-04-06T20:11:24Z<p>Bussand1: /* Cell Structure, Metabolism and Life Cycle */</p>
<hr />
<div>[[Image:AlcVorax PROCARYOTES01b-1.JPG|thumbnail|300px|Figure 1. ''Alcanivorax borkumensis''. Image from Helmholtz Centre for Infection Research[http://www.helmholtz-hzi.de/en/news_public_relation/press_releases/view/article/complete/oil_tanker_accidents_as_a_source_of_food/]]]<br />
<br />
==Classification==<br />
<br />
'''Bacteria'''; Phylum: '''Proteobacteria'''; Class: '''Gammaproteobacteria'''; Order: '''Oceanospirillales'''; Family: '''Alcanivoracaceae'''<br />
<br />
===Species===<br />
<br />
{|<br />
| height="10" bgcolor="#FFDF95" |<br />
'''NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Tree&id=2&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy]'''<br />
|}<br />
<br />
*''Alcanivorax balearicum''<br />
*''Alcanivorax borkumensis''<br />
*''Alcanivorax dieselolei''<br />
*''Alcanivorax indicus''<br />
*''Alcanivorax jadensis''<br />
*''Alcanivorax venustensis''<br />
<br />
==Description and Significance==<br />
[[Image:PWS tanker oil spill.jpg|thumbnail|200px|Figure 2. Supertanker Exxon Valdez grounded on Bligh Reef which released 11 million gallons of crude oil into the water. This oil-contaminated seawater is the preferred habitat for ''Alcanivorax''. Image from USGS[http://menlocampus.wr.usgs.gov/50years/accomplishments/oil.html]]]<br />
<br />
''Alcanivorax'', first described in 1998, is a Gram-negative, halophilic, aerobic, rod-shaped, oil-degrading marine bacterium that is found in low abundances in unpolluted environments in the upper layers of the ocean, but quickly becomes the predominant microbe in oil-contaminated open oceans and coastal waters when nitrogen and phosphorus are not limiting [2]. When conditions in these moderately halophilic environments are right, ''Alcanivorax'' may make up 80-90% of the oil-degrading microbes present in the area [4]. It is described as a non-motile bactertium which is true for species such as ''Alcanivorax borkumensis'', but other species such as ''Alcanivorax venustensis'' were described to be motile by polar flagella [1]. <br />
<br />
As a result of their profound ability to degrade and live predominately on alkanes, as well as to become the dominant microbes in oil-contaminated areas, ''Alcanivorax'' plays a huge role in the biological cleanup of oil-contaminated environments. These oil-contaminated environments in the ocean are largely due to anthropogenic sources such as oil spills caused by tankers accidents, and cause serious ecological damage to coastal fauna and flora as well as other inhabitants of the ocean. Microbes such as ''Alcanivorax'' provide a major route for the breakdown of these pollutants, and demonstrate how marine bacteria keep the environment in check. Of all the ''Alcanivorax'' species and other oil-degrading microbes, ''Alcanivorax borkumensis'' is one of the most important worldwide due to the fact it produces a wide variety of very efficient oil-degrading enzymes. With this knowledge, ''A. borkumensis'' could provide a useful tool for bioremediation of oil spills.<br />
<br />
==Genome Structure==<br />
[[Image:Lorenzo.gif|thumbnail|200px|Figure 3. Mechanisms for oil degradation and survival encoded by the ''A. borkumensis SK2'' genome. Image from Victor de Lorenzo[http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html]]]<br />
<br />
The ''Alcanivorax borkumensis'' strain SK2, isolated from a seawater sediment sample in the North Sea at a site located near the Isle of Borkum, was the first hydrocarbonoclastic bacterium to be sequenced and was completed by Susanne Schneiker et al. It's genome consists of a single circular chromosome with 3,120,143 base pairs with an average G+C content of 54.7%. The genomic analysis of ''A. borkumensis SK2'' revealed several new insights into the bacterium's role for (i) n-alkane degradation (which includes metabolism, biosurfactant production and biofilm production), (ii) it's system for capturing or scavenging the small amounts of nitrogen, phosphorous, sulfur, and other elements in a nutrient-poor marine environment which allows for more efficient alkane degradation due to their main limitation of nutrient availability, (iii) as well as means for coping with stress factors such as high salt contents and high UV radiation since it thrives mostly in the upper layers in the ocean where UV light is encountered. It's genome encodes several systems for the catabolism of hydrocarbons which allow the bacertium to degrade all sorts of alkanes such as AlkB1 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C5-C12, and AlkB2 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C8 to C16. Both these systems are located close to the origin of replication of the chromosome. ''A. borkumensis'' is also able to degrade alkanes up to C32, branched aliphatic hydrocarbons, isoprenoid hydrocarbons such as phytane, as well as alkylarenes and alkylcycloalkanes. Thus, the genome encodes for a broad spectrum of systems for the catabolism of hydrocarbons, giving it a competitive advantage over other oil-degrading marine microbial communities. To deal with the damaging effects of UV light, ''A. borkumensis'' has a number of genes that reduce the damage. These include the full genes for DNA alkylation, recombinational and nucleotide excision repair, base excision repair, as well as the SOS response [4].<br />
<br />
==Cell Structure, Metabolism and Life Cycle==<br />
Interesting features of cell structure; how it gains energy; what important molecules it produces.<br />
<br />
Alcanivorax borkumensis is able to use n-alkanes as its principle carbon and energy source by use of the broad spectrum of oil-degrading enzymes it possesses, but they can also use a limited number of organic compounds such as aliphatic hydrocarbons, volatile fatty acids, and pyruvate. However, it cannot utilize carbon sources such as sugars or amino acids. When the slow growing ''A. borkumensis'' uses n-alkanes exclusively, the microbes produce extracellular and membrane-bound surface-active glucose lipids called biosurfactants. These biosurfactants act as natural emulsifiers which enhances the break up of oil-in-water emulsions which prevent degradation of alkanes [4]. Due to the low solubility of oil in water, most oil degradation takes place at the oil-water interface where ''A. borkumensis'' attaches and forms a biofilm around the oil droplets as depicted in Figure 3.<br />
<br />
==Ecology and Pathogenesis==<br />
Habitat; symbiosis; biogeochemical significance; contributions to environment.<br><br />
If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.<br><br><br />
<br />
==References==<br />
[1] [http://ijs.sgmjournals.org/cgi/content/abstract/53/1/331 Fernandez-Martinez, Javier, Maria J. Pujalte, Jesus Garcia-Martinez, Manuel Mata, Esperanza Garay, and Francisco Rodriguez-Valera. "Description of ''Alcanivorax Venustensis'' sp. nov. and Reclassification of ''Fundibacter Jadensis'' DSM 12178T (Bruns and Berthe-Corti 1999) As ''Alcanivorax Jadensis'' comb. nov., Members of the Emended Genus ''Alcanivorax''." International Journal of Systematic and Evolutionary Microbiology 53 (2003): 331-338.]<br />
<br />
[2] [http://www.blackwell-synergy.com/doi/pdf/10.1046/j.1468-2920.2003.00468.x Hara, Akihiro, Kazuaki Syutsubo, and Shigeaki Harayama. "''Alcanivorax'' Which Prevails In Oil-contaminated Seawater Exhibits Broad Substrate Specificity For Alkane Degradation." Environmental Microbiology 5.9 (2003): 746-753.]<br />
<br />
[3] [http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html Lorenzo, Víctor De. "Blueprint of an Oil-eating Bacterium." Nature Biotechnology 24 (2006): 952-953.]<br />
<br />
[4] [http://www.ncbi.nlm.nih.gov/pubmed/16878126 Schneiker, S. et al. "Genome Sequence of the Ubiquitous Hydrocarbon-degrading Marine Bacterium A''lcanivorax Borkumensis''." Nature Biotechnology 24 (2006): 997-1004.]<br />
<br />
[5] [http://ijs.sgmjournals.org/cgi/content/abstract/48/2/339 Yakimov, Michail M., Peter N. Golyshin, Siegmund Lang, Edward R. B. Moore, Wolf-Rainer Abraham, Heinrich Lunsdorf, and Kenneth N. Timmis. "''Alcanivorax Borkumensis'' gen. nov., sp. nov., A New, Hydrocarbon-degrading And Surfactant-producing Marine Bacterium." International Journal of Systematic Bacteriology 48 (1998): 339-348.]<br />
<br />
==Author==<br />
Page authored by Andrew Buss, student of [http://www.kbs.msu.edu/faculty/lennon/ Prof. Jay Lennon] at Michigan State University.</div>Bussand1https://microbewiki.kenyon.edu/index.php?title=Alcanivorax&diff=29744Alcanivorax2008-04-06T20:08:28Z<p>Bussand1: /* Cell Structure, Metabolism and Life Cycle */</p>
<hr />
<div>[[Image:AlcVorax PROCARYOTES01b-1.JPG|thumbnail|300px|Figure 1. ''Alcanivorax borkumensis''. Image from Helmholtz Centre for Infection Research[http://www.helmholtz-hzi.de/en/news_public_relation/press_releases/view/article/complete/oil_tanker_accidents_as_a_source_of_food/]]]<br />
<br />
==Classification==<br />
<br />
'''Bacteria'''; Phylum: '''Proteobacteria'''; Class: '''Gammaproteobacteria'''; Order: '''Oceanospirillales'''; Family: '''Alcanivoracaceae'''<br />
<br />
===Species===<br />
<br />
{|<br />
| height="10" bgcolor="#FFDF95" |<br />
'''NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Tree&id=2&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy]'''<br />
|}<br />
<br />
*''Alcanivorax balearicum''<br />
*''Alcanivorax borkumensis''<br />
*''Alcanivorax dieselolei''<br />
*''Alcanivorax indicus''<br />
*''Alcanivorax jadensis''<br />
*''Alcanivorax venustensis''<br />
<br />
==Description and Significance==<br />
[[Image:PWS tanker oil spill.jpg|thumbnail|200px|Figure 2. Supertanker Exxon Valdez grounded on Bligh Reef which released 11 million gallons of crude oil into the water. This oil-contaminated seawater is the preferred habitat for ''Alcanivorax''. Image from USGS[http://menlocampus.wr.usgs.gov/50years/accomplishments/oil.html]]]<br />
<br />
''Alcanivorax'', first described in 1998, is a Gram-negative, halophilic, aerobic, rod-shaped, oil-degrading marine bacterium that is found in low abundances in unpolluted environments in the upper layers of the ocean, but quickly becomes the predominant microbe in oil-contaminated open oceans and coastal waters when nitrogen and phosphorus are not limiting [2]. When conditions in these moderately halophilic environments are right, ''Alcanivorax'' may make up 80-90% of the oil-degrading microbes present in the area [4]. It is described as a non-motile bactertium which is true for species such as ''Alcanivorax borkumensis'', but other species such as ''Alcanivorax venustensis'' were described to be motile by polar flagella [1]. <br />
<br />
As a result of their profound ability to degrade and live predominately on alkanes, as well as to become the dominant microbes in oil-contaminated areas, ''Alcanivorax'' plays a huge role in the biological cleanup of oil-contaminated environments. These oil-contaminated environments in the ocean are largely due to anthropogenic sources such as oil spills caused by tankers accidents, and cause serious ecological damage to coastal fauna and flora as well as other inhabitants of the ocean. Microbes such as ''Alcanivorax'' provide a major route for the breakdown of these pollutants, and demonstrate how marine bacteria keep the environment in check. Of all the ''Alcanivorax'' species and other oil-degrading microbes, ''Alcanivorax borkumensis'' is one of the most important worldwide due to the fact it produces a wide variety of very efficient oil-degrading enzymes. With this knowledge, ''A. borkumensis'' could provide a useful tool for bioremediation of oil spills.<br />
<br />
==Genome Structure==<br />
[[Image:Lorenzo.gif|thumbnail|200px|Figure 3. Mechanisms for oil degradation and survival encoded by the ''A. borkumensis SK2'' genome. Image from Victor de Lorenzo[http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html]]]<br />
<br />
The ''Alcanivorax borkumensis'' strain SK2, isolated from a seawater sediment sample in the North Sea at a site located near the Isle of Borkum, was the first hydrocarbonoclastic bacterium to be sequenced and was completed by Susanne Schneiker et al. It's genome consists of a single circular chromosome with 3,120,143 base pairs with an average G+C content of 54.7%. The genomic analysis of ''A. borkumensis SK2'' revealed several new insights into the bacterium's role for (i) n-alkane degradation (which includes metabolism, biosurfactant production and biofilm production), (ii) it's system for capturing or scavenging the small amounts of nitrogen, phosphorous, sulfur, and other elements in a nutrient-poor marine environment which allows for more efficient alkane degradation due to their main limitation of nutrient availability, (iii) as well as means for coping with stress factors such as high salt contents and high UV radiation since it thrives mostly in the upper layers in the ocean where UV light is encountered. It's genome encodes several systems for the catabolism of hydrocarbons which allow the bacertium to degrade all sorts of alkanes such as AlkB1 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C5-C12, and AlkB2 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C8 to C16. Both these systems are located close to the origin of replication of the chromosome. ''A. borkumensis'' is also able to degrade alkanes up to C32, branched aliphatic hydrocarbons, isoprenoid hydrocarbons such as phytane, as well as alkylarenes and alkylcycloalkanes. Thus, the genome encodes for a broad spectrum of systems for the catabolism of hydrocarbons, giving it a competitive advantage over other oil-degrading marine microbial communities. To deal with the damaging effects of UV light, ''A. borkumensis'' has a number of genes that reduce the damage. These include the full genes for DNA alkylation, recombinational and nucleotide excision repair, base excision repair, as well as the SOS response [4].<br />
<br />
==Cell Structure, Metabolism and Life Cycle==<br />
Interesting features of cell structure; how it gains energy; what important molecules it produces.<br />
<br />
Alcanivorax borkumensis is able to use n-alkanes as its principle carbon and energy source by use of the broad spectrum of oil-degrading enzymes it possesses, but they can also use a limited number of organic compounds such as aliphatic hydrocarbons, volatile fatty acids, and pyruvate. However, it cannot utilize carbon sources such as sugars or amino acids. When the slow growing ''A. borkumensis'' uses n-alkanes exclusively, the microbes produce extracellular and membrane-bound surface-active glucose lipids called biosurfactants. These biosurfactants act as natural emulsifiers which enhances the break up of oil-in-water emulsions which prevent degradation of alkanes [4]. Due to the low solubility of oil in water, most oil degradation takes place at the oil-water interface as depicted in Figure 3.<br />
<br />
==Ecology and Pathogenesis==<br />
Habitat; symbiosis; biogeochemical significance; contributions to environment.<br><br />
If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.<br><br><br />
<br />
==References==<br />
[1] [http://ijs.sgmjournals.org/cgi/content/abstract/53/1/331 Fernandez-Martinez, Javier, Maria J. Pujalte, Jesus Garcia-Martinez, Manuel Mata, Esperanza Garay, and Francisco Rodriguez-Valera. "Description of ''Alcanivorax Venustensis'' sp. nov. and Reclassification of ''Fundibacter Jadensis'' DSM 12178T (Bruns and Berthe-Corti 1999) As ''Alcanivorax Jadensis'' comb. nov., Members of the Emended Genus ''Alcanivorax''." International Journal of Systematic and Evolutionary Microbiology 53 (2003): 331-338.]<br />
<br />
[2] [http://www.blackwell-synergy.com/doi/pdf/10.1046/j.1468-2920.2003.00468.x Hara, Akihiro, Kazuaki Syutsubo, and Shigeaki Harayama. "''Alcanivorax'' Which Prevails In Oil-contaminated Seawater Exhibits Broad Substrate Specificity For Alkane Degradation." Environmental Microbiology 5.9 (2003): 746-753.]<br />
<br />
[3] [http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html Lorenzo, Víctor De. "Blueprint of an Oil-eating Bacterium." Nature Biotechnology 24 (2006): 952-953.]<br />
<br />
[4] [http://www.ncbi.nlm.nih.gov/pubmed/16878126 Schneiker, S. et al. "Genome Sequence of the Ubiquitous Hydrocarbon-degrading Marine Bacterium A''lcanivorax Borkumensis''." Nature Biotechnology 24 (2006): 997-1004.]<br />
<br />
[5] [http://ijs.sgmjournals.org/cgi/content/abstract/48/2/339 Yakimov, Michail M., Peter N. Golyshin, Siegmund Lang, Edward R. B. Moore, Wolf-Rainer Abraham, Heinrich Lunsdorf, and Kenneth N. Timmis. "''Alcanivorax Borkumensis'' gen. nov., sp. nov., A New, Hydrocarbon-degrading And Surfactant-producing Marine Bacterium." International Journal of Systematic Bacteriology 48 (1998): 339-348.]<br />
<br />
==Author==<br />
Page authored by Andrew Buss, student of [http://www.kbs.msu.edu/faculty/lennon/ Prof. Jay Lennon] at Michigan State University.</div>Bussand1https://microbewiki.kenyon.edu/index.php?title=Alcanivorax&diff=29743Alcanivorax2008-04-06T20:08:03Z<p>Bussand1: /* Genome Structure */</p>
<hr />
<div>[[Image:AlcVorax PROCARYOTES01b-1.JPG|thumbnail|300px|Figure 1. ''Alcanivorax borkumensis''. Image from Helmholtz Centre for Infection Research[http://www.helmholtz-hzi.de/en/news_public_relation/press_releases/view/article/complete/oil_tanker_accidents_as_a_source_of_food/]]]<br />
<br />
==Classification==<br />
<br />
'''Bacteria'''; Phylum: '''Proteobacteria'''; Class: '''Gammaproteobacteria'''; Order: '''Oceanospirillales'''; Family: '''Alcanivoracaceae'''<br />
<br />
===Species===<br />
<br />
{|<br />
| height="10" bgcolor="#FFDF95" |<br />
'''NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Tree&id=2&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy]'''<br />
|}<br />
<br />
*''Alcanivorax balearicum''<br />
*''Alcanivorax borkumensis''<br />
*''Alcanivorax dieselolei''<br />
*''Alcanivorax indicus''<br />
*''Alcanivorax jadensis''<br />
*''Alcanivorax venustensis''<br />
<br />
==Description and Significance==<br />
[[Image:PWS tanker oil spill.jpg|thumbnail|200px|Figure 2. Supertanker Exxon Valdez grounded on Bligh Reef which released 11 million gallons of crude oil into the water. This oil-contaminated seawater is the preferred habitat for ''Alcanivorax''. Image from USGS[http://menlocampus.wr.usgs.gov/50years/accomplishments/oil.html]]]<br />
<br />
''Alcanivorax'', first described in 1998, is a Gram-negative, halophilic, aerobic, rod-shaped, oil-degrading marine bacterium that is found in low abundances in unpolluted environments in the upper layers of the ocean, but quickly becomes the predominant microbe in oil-contaminated open oceans and coastal waters when nitrogen and phosphorus are not limiting [2]. When conditions in these moderately halophilic environments are right, ''Alcanivorax'' may make up 80-90% of the oil-degrading microbes present in the area [4]. It is described as a non-motile bactertium which is true for species such as ''Alcanivorax borkumensis'', but other species such as ''Alcanivorax venustensis'' were described to be motile by polar flagella [1]. <br />
<br />
As a result of their profound ability to degrade and live predominately on alkanes, as well as to become the dominant microbes in oil-contaminated areas, ''Alcanivorax'' plays a huge role in the biological cleanup of oil-contaminated environments. These oil-contaminated environments in the ocean are largely due to anthropogenic sources such as oil spills caused by tankers accidents, and cause serious ecological damage to coastal fauna and flora as well as other inhabitants of the ocean. Microbes such as ''Alcanivorax'' provide a major route for the breakdown of these pollutants, and demonstrate how marine bacteria keep the environment in check. Of all the ''Alcanivorax'' species and other oil-degrading microbes, ''Alcanivorax borkumensis'' is one of the most important worldwide due to the fact it produces a wide variety of very efficient oil-degrading enzymes. With this knowledge, ''A. borkumensis'' could provide a useful tool for bioremediation of oil spills.<br />
<br />
==Genome Structure==<br />
[[Image:Lorenzo.gif|thumbnail|200px|Figure 3. Mechanisms for oil degradation and survival encoded by the ''A. borkumensis SK2'' genome. Image from Victor de Lorenzo[http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html]]]<br />
<br />
The ''Alcanivorax borkumensis'' strain SK2, isolated from a seawater sediment sample in the North Sea at a site located near the Isle of Borkum, was the first hydrocarbonoclastic bacterium to be sequenced and was completed by Susanne Schneiker et al. It's genome consists of a single circular chromosome with 3,120,143 base pairs with an average G+C content of 54.7%. The genomic analysis of ''A. borkumensis SK2'' revealed several new insights into the bacterium's role for (i) n-alkane degradation (which includes metabolism, biosurfactant production and biofilm production), (ii) it's system for capturing or scavenging the small amounts of nitrogen, phosphorous, sulfur, and other elements in a nutrient-poor marine environment which allows for more efficient alkane degradation due to their main limitation of nutrient availability, (iii) as well as means for coping with stress factors such as high salt contents and high UV radiation since it thrives mostly in the upper layers in the ocean where UV light is encountered. It's genome encodes several systems for the catabolism of hydrocarbons which allow the bacertium to degrade all sorts of alkanes such as AlkB1 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C5-C12, and AlkB2 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C8 to C16. Both these systems are located close to the origin of replication of the chromosome. ''A. borkumensis'' is also able to degrade alkanes up to C32, branched aliphatic hydrocarbons, isoprenoid hydrocarbons such as phytane, as well as alkylarenes and alkylcycloalkanes. Thus, the genome encodes for a broad spectrum of systems for the catabolism of hydrocarbons, giving it a competitive advantage over other oil-degrading marine microbial communities. To deal with the damaging effects of UV light, ''A. borkumensis'' has a number of genes that reduce the damage. These include the full genes for DNA alkylation, recombinational and nucleotide excision repair, base excision repair, as well as the SOS response [4].<br />
<br />
==Cell Structure, Metabolism and Life Cycle==<br />
Interesting features of cell structure; how it gains energy; what important molecules it produces.<br />
<br />
Alcanivorax borkumensis is able to use n-alkanes as its principle carbon and energy source by use of the broad spectrum of oil-degrading enzymes it possesses, but they can also use a limited number of organic compounds such as aliphatic hydrocarbons, volatile fatty acids, and pyruvate. However, it cannot utilize carbon sources such as sugars or amino acids. When the slow growing ''A. borkumensis'' uses n-alkanes exclusively, the microbes produce extracellular and membrane-bound surface-active glucose lipids called biosurfactants. These biosurfactants act as natural emulsifiers which enhances the break up of oil-in-water emulsions which prevent degradation of alkanes (Schneiker et al., 2006). Due to the low solubility of oil in water, most oil degradation takes place at the oil-water interface as depicted in figure 3.<br />
<br />
==Ecology and Pathogenesis==<br />
Habitat; symbiosis; biogeochemical significance; contributions to environment.<br><br />
If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.<br><br><br />
<br />
==References==<br />
[1] [http://ijs.sgmjournals.org/cgi/content/abstract/53/1/331 Fernandez-Martinez, Javier, Maria J. Pujalte, Jesus Garcia-Martinez, Manuel Mata, Esperanza Garay, and Francisco Rodriguez-Valera. "Description of ''Alcanivorax Venustensis'' sp. nov. and Reclassification of ''Fundibacter Jadensis'' DSM 12178T (Bruns and Berthe-Corti 1999) As ''Alcanivorax Jadensis'' comb. nov., Members of the Emended Genus ''Alcanivorax''." International Journal of Systematic and Evolutionary Microbiology 53 (2003): 331-338.]<br />
<br />
[2] [http://www.blackwell-synergy.com/doi/pdf/10.1046/j.1468-2920.2003.00468.x Hara, Akihiro, Kazuaki Syutsubo, and Shigeaki Harayama. "''Alcanivorax'' Which Prevails In Oil-contaminated Seawater Exhibits Broad Substrate Specificity For Alkane Degradation." Environmental Microbiology 5.9 (2003): 746-753.]<br />
<br />
[3] [http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html Lorenzo, Víctor De. "Blueprint of an Oil-eating Bacterium." Nature Biotechnology 24 (2006): 952-953.]<br />
<br />
[4] [http://www.ncbi.nlm.nih.gov/pubmed/16878126 Schneiker, S. et al. "Genome Sequence of the Ubiquitous Hydrocarbon-degrading Marine Bacterium A''lcanivorax Borkumensis''." Nature Biotechnology 24 (2006): 997-1004.]<br />
<br />
[5] [http://ijs.sgmjournals.org/cgi/content/abstract/48/2/339 Yakimov, Michail M., Peter N. Golyshin, Siegmund Lang, Edward R. B. Moore, Wolf-Rainer Abraham, Heinrich Lunsdorf, and Kenneth N. Timmis. "''Alcanivorax Borkumensis'' gen. nov., sp. nov., A New, Hydrocarbon-degrading And Surfactant-producing Marine Bacterium." International Journal of Systematic Bacteriology 48 (1998): 339-348.]<br />
<br />
==Author==<br />
Page authored by Andrew Buss, student of [http://www.kbs.msu.edu/faculty/lennon/ Prof. Jay Lennon] at Michigan State University.</div>Bussand1https://microbewiki.kenyon.edu/index.php?title=Alcanivorax&diff=29742Alcanivorax2008-04-06T20:07:44Z<p>Bussand1: /* Description and Significance */</p>
<hr />
<div>[[Image:AlcVorax PROCARYOTES01b-1.JPG|thumbnail|300px|Figure 1. ''Alcanivorax borkumensis''. Image from Helmholtz Centre for Infection Research[http://www.helmholtz-hzi.de/en/news_public_relation/press_releases/view/article/complete/oil_tanker_accidents_as_a_source_of_food/]]]<br />
<br />
==Classification==<br />
<br />
'''Bacteria'''; Phylum: '''Proteobacteria'''; Class: '''Gammaproteobacteria'''; Order: '''Oceanospirillales'''; Family: '''Alcanivoracaceae'''<br />
<br />
===Species===<br />
<br />
{|<br />
| height="10" bgcolor="#FFDF95" |<br />
'''NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Tree&id=2&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy]'''<br />
|}<br />
<br />
*''Alcanivorax balearicum''<br />
*''Alcanivorax borkumensis''<br />
*''Alcanivorax dieselolei''<br />
*''Alcanivorax indicus''<br />
*''Alcanivorax jadensis''<br />
*''Alcanivorax venustensis''<br />
<br />
==Description and Significance==<br />
[[Image:PWS tanker oil spill.jpg|thumbnail|200px|Figure 2. Supertanker Exxon Valdez grounded on Bligh Reef which released 11 million gallons of crude oil into the water. This oil-contaminated seawater is the preferred habitat for ''Alcanivorax''. Image from USGS[http://menlocampus.wr.usgs.gov/50years/accomplishments/oil.html]]]<br />
<br />
''Alcanivorax'', first described in 1998, is a Gram-negative, halophilic, aerobic, rod-shaped, oil-degrading marine bacterium that is found in low abundances in unpolluted environments in the upper layers of the ocean, but quickly becomes the predominant microbe in oil-contaminated open oceans and coastal waters when nitrogen and phosphorus are not limiting [2]. When conditions in these moderately halophilic environments are right, ''Alcanivorax'' may make up 80-90% of the oil-degrading microbes present in the area [4]. It is described as a non-motile bactertium which is true for species such as ''Alcanivorax borkumensis'', but other species such as ''Alcanivorax venustensis'' were described to be motile by polar flagella [1]. <br />
<br />
As a result of their profound ability to degrade and live predominately on alkanes, as well as to become the dominant microbes in oil-contaminated areas, ''Alcanivorax'' plays a huge role in the biological cleanup of oil-contaminated environments. These oil-contaminated environments in the ocean are largely due to anthropogenic sources such as oil spills caused by tankers accidents, and cause serious ecological damage to coastal fauna and flora as well as other inhabitants of the ocean. Microbes such as ''Alcanivorax'' provide a major route for the breakdown of these pollutants, and demonstrate how marine bacteria keep the environment in check. Of all the ''Alcanivorax'' species and other oil-degrading microbes, ''Alcanivorax borkumensis'' is one of the most important worldwide due to the fact it produces a wide variety of very efficient oil-degrading enzymes. With this knowledge, ''A. borkumensis'' could provide a useful tool for bioremediation of oil spills.<br />
<br />
==Genome Structure==<br />
[[Image:Lorenzo.gif|thumbnail|200px|Figure 3. Mechanisms for oil degradation and survival encoded by the ''A. borkumensis SK2'' genome. Image from Victor de Lorenzo[http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html]]]<br />
<br />
The ''Alcanivorax borkumensis'' strain SK2, isolated from a seawater sediment sample in the North Sea at a site located near the Isle of Borkum, was the first hydrocarbonoclastic bacterium to be sequenced and was completed by Susanne Schneiker et al. It's genome consists of a single circular chromosome with 3,120,143 base pairs with an average G+C content of 54.7%. The genomic analysis of ''A. borkumensis SK2'' revealed several new insights into the bacterium's role for (i) n-alkane degradation (which includes metabolism, biosurfactant production and biofilm production), (ii) it's system for capturing or scavenging the small amounts of nitrogen, phosphorous, sulfur, and other elements in a nutrient-poor marine environment which allows for more efficient alkane degradation due to their main limitation of nutrient availability, (iii) as well as means for coping with stress factors such as high salt contents and high UV radiation since it thrives mostly in the upper layers in the ocean where UV light is encountered. It's genome encodes several systems for the catabolism of hydrocarbons which allow the bacertium to degrade all sorts of alkanes such as AlkB1 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C5-C12, and AlkB2 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C8 to C16. Both these systems are located close to the origin of replication of the chromosome. ''A. borkumensis'' is also able to degrade alkanes up to C32, branched aliphatic hydrocarbons, isoprenoid hydrocarbons such as phytane, as well as alkylarenes and alkylcycloalkanes. Thus, the genome encodes for a broad spectrum of systems for the catabolism of hydrocarbons, giving it a competitive advantage over other oil-degrading marine microbial communities. To deal with the damaging effects of UV light, ''A. borkumensis'' has a number of genes that reduce the damage. These include the full genes for DNA alkylation, recombinational and nucleotide excision repair, base excision repair, as well as the SOS response (Schneiker et al., 2006).<br />
<br />
==Cell Structure, Metabolism and Life Cycle==<br />
Interesting features of cell structure; how it gains energy; what important molecules it produces.<br />
<br />
Alcanivorax borkumensis is able to use n-alkanes as its principle carbon and energy source by use of the broad spectrum of oil-degrading enzymes it possesses, but they can also use a limited number of organic compounds such as aliphatic hydrocarbons, volatile fatty acids, and pyruvate. However, it cannot utilize carbon sources such as sugars or amino acids. When the slow growing ''A. borkumensis'' uses n-alkanes exclusively, the microbes produce extracellular and membrane-bound surface-active glucose lipids called biosurfactants. These biosurfactants act as natural emulsifiers which enhances the break up of oil-in-water emulsions which prevent degradation of alkanes (Schneiker et al., 2006). Due to the low solubility of oil in water, most oil degradation takes place at the oil-water interface as depicted in figure 3.<br />
<br />
==Ecology and Pathogenesis==<br />
Habitat; symbiosis; biogeochemical significance; contributions to environment.<br><br />
If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.<br><br><br />
<br />
==References==<br />
[1] [http://ijs.sgmjournals.org/cgi/content/abstract/53/1/331 Fernandez-Martinez, Javier, Maria J. Pujalte, Jesus Garcia-Martinez, Manuel Mata, Esperanza Garay, and Francisco Rodriguez-Valera. "Description of ''Alcanivorax Venustensis'' sp. nov. and Reclassification of ''Fundibacter Jadensis'' DSM 12178T (Bruns and Berthe-Corti 1999) As ''Alcanivorax Jadensis'' comb. nov., Members of the Emended Genus ''Alcanivorax''." International Journal of Systematic and Evolutionary Microbiology 53 (2003): 331-338.]<br />
<br />
[2] [http://www.blackwell-synergy.com/doi/pdf/10.1046/j.1468-2920.2003.00468.x Hara, Akihiro, Kazuaki Syutsubo, and Shigeaki Harayama. "''Alcanivorax'' Which Prevails In Oil-contaminated Seawater Exhibits Broad Substrate Specificity For Alkane Degradation." Environmental Microbiology 5.9 (2003): 746-753.]<br />
<br />
[3] [http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html Lorenzo, Víctor De. "Blueprint of an Oil-eating Bacterium." Nature Biotechnology 24 (2006): 952-953.]<br />
<br />
[4] [http://www.ncbi.nlm.nih.gov/pubmed/16878126 Schneiker, S. et al. "Genome Sequence of the Ubiquitous Hydrocarbon-degrading Marine Bacterium A''lcanivorax Borkumensis''." Nature Biotechnology 24 (2006): 997-1004.]<br />
<br />
[5] [http://ijs.sgmjournals.org/cgi/content/abstract/48/2/339 Yakimov, Michail M., Peter N. Golyshin, Siegmund Lang, Edward R. B. Moore, Wolf-Rainer Abraham, Heinrich Lunsdorf, and Kenneth N. Timmis. "''Alcanivorax Borkumensis'' gen. nov., sp. nov., A New, Hydrocarbon-degrading And Surfactant-producing Marine Bacterium." International Journal of Systematic Bacteriology 48 (1998): 339-348.]<br />
<br />
==Author==<br />
Page authored by Andrew Buss, student of [http://www.kbs.msu.edu/faculty/lennon/ Prof. Jay Lennon] at Michigan State University.</div>Bussand1https://microbewiki.kenyon.edu/index.php?title=Alcanivorax&diff=29741Alcanivorax2008-04-06T20:03:57Z<p>Bussand1: /* Cell Structure, Metabolism and Life Cycle */</p>
<hr />
<div>[[Image:AlcVorax PROCARYOTES01b-1.JPG|thumbnail|300px|Figure 1. ''Alcanivorax borkumensis''. Image from Helmholtz Centre for Infection Research[http://www.helmholtz-hzi.de/en/news_public_relation/press_releases/view/article/complete/oil_tanker_accidents_as_a_source_of_food/]]]<br />
<br />
==Classification==<br />
<br />
'''Bacteria'''; Phylum: '''Proteobacteria'''; Class: '''Gammaproteobacteria'''; Order: '''Oceanospirillales'''; Family: '''Alcanivoracaceae'''<br />
<br />
===Species===<br />
<br />
{|<br />
| height="10" bgcolor="#FFDF95" |<br />
'''NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Tree&id=2&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy]'''<br />
|}<br />
<br />
*''Alcanivorax balearicum''<br />
*''Alcanivorax borkumensis''<br />
*''Alcanivorax dieselolei''<br />
*''Alcanivorax indicus''<br />
*''Alcanivorax jadensis''<br />
*''Alcanivorax venustensis''<br />
<br />
==Description and Significance==<br />
[[Image:PWS tanker oil spill.jpg|thumbnail|200px|Figure 2. Supertanker Exxon Valdez grounded on Bligh Reef which released 11 million gallons of crude oil into the water. This oil-contaminated seawater is the preferred habitat for ''Alcanivorax''. Image from USGS[http://menlocampus.wr.usgs.gov/50years/accomplishments/oil.html]]]<br />
<br />
''Alcanivorax'', first described in 1998, is a Gram-negative, halophilic, aerobic, rod-shaped, oil-degrading marine bacterium that is found in low abundances in unpolluted environments in the upper layers of the ocean, but quickly becomes the predominant microbe in oil-contaminated open oceans and coastal waters when nitrogen and phosphorus are not limiting (Hara, 2003). When conditions in these moderately halophilic environments are right, ''Alcanivorax'' may make up 80-90% of the oil-degrading microbes present in the area (Schneiker et al., 2006). It is described as a non-motile bactertium which is true for species such as ''Alcanivorax borkumensis'', but other species such as ''Alcanivorax venustensis'' were described to be motile by polar flagella (Fernandez-Martinez et al., 2003). <br />
<br />
As a result of their profound ability to degrade and live predominately on alkanes, as well as to become the dominant microbes in oil-contaminated areas, ''Alcanivorax'' plays a huge role in the biological cleanup of oil-contaminated environments. These oil-contaminated environments in the ocean are largely due to anthropogenic sources such as oil spills caused by tankers accidents, and cause serious ecological damage to coastal fauna and flora as well as other inhabitants of the ocean. Microbes such as ''Alcanivorax'' provide a major route for the breakdown of these pollutants, and demonstrate how marine bacteria keep the environment in check. Of all the ''Alcanivorax'' species and other oil-degrading microbes, ''Alcanivorax borkumensis'' is one of the most important worldwide due to the fact it produces a wide variety of very efficient oil-degrading enzymes. With this knowledge, ''A. borkumensis'' could provide a useful tool for bioremediation of oil spills.<br />
<br />
==Genome Structure==<br />
[[Image:Lorenzo.gif|thumbnail|200px|Figure 3. Mechanisms for oil degradation and survival encoded by the ''A. borkumensis SK2'' genome. Image from Victor de Lorenzo[http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html]]]<br />
<br />
The ''Alcanivorax borkumensis'' strain SK2, isolated from a seawater sediment sample in the North Sea at a site located near the Isle of Borkum, was the first hydrocarbonoclastic bacterium to be sequenced and was completed by Susanne Schneiker et al. It's genome consists of a single circular chromosome with 3,120,143 base pairs with an average G+C content of 54.7%. The genomic analysis of ''A. borkumensis SK2'' revealed several new insights into the bacterium's role for (i) n-alkane degradation (which includes metabolism, biosurfactant production and biofilm production), (ii) it's system for capturing or scavenging the small amounts of nitrogen, phosphorous, sulfur, and other elements in a nutrient-poor marine environment which allows for more efficient alkane degradation due to their main limitation of nutrient availability, (iii) as well as means for coping with stress factors such as high salt contents and high UV radiation since it thrives mostly in the upper layers in the ocean where UV light is encountered. It's genome encodes several systems for the catabolism of hydrocarbons which allow the bacertium to degrade all sorts of alkanes such as AlkB1 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C5-C12, and AlkB2 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C8 to C16. Both these systems are located close to the origin of replication of the chromosome. ''A. borkumensis'' is also able to degrade alkanes up to C32, branched aliphatic hydrocarbons, isoprenoid hydrocarbons such as phytane, as well as alkylarenes and alkylcycloalkanes. Thus, the genome encodes for a broad spectrum of systems for the catabolism of hydrocarbons, giving it a competitive advantage over other oil-degrading marine microbial communities. To deal with the damaging effects of UV light, ''A. borkumensis'' has a number of genes that reduce the damage. These include the full genes for DNA alkylation, recombinational and nucleotide excision repair, base excision repair, as well as the SOS response (Schneiker et al., 2006).<br />
<br />
==Cell Structure, Metabolism and Life Cycle==<br />
Interesting features of cell structure; how it gains energy; what important molecules it produces.<br />
<br />
Alcanivorax borkumensis is able to use n-alkanes as its principle carbon and energy source by use of the broad spectrum of oil-degrading enzymes it possesses, but they can also use a limited number of organic compounds such as aliphatic hydrocarbons, volatile fatty acids, and pyruvate. However, it cannot utilize carbon sources such as sugars or amino acids. When the slow growing ''A. borkumensis'' uses n-alkanes exclusively, the microbes produce extracellular and membrane-bound surface-active glucose lipids called biosurfactants. These biosurfactants act as natural emulsifiers which enhances the break up of oil-in-water emulsions which prevent degradation of alkanes (Schneiker et al., 2006). Due to the low solubility of oil in water, most oil degradation takes place at the oil-water interface as depicted in figure 3.<br />
<br />
==Ecology and Pathogenesis==<br />
Habitat; symbiosis; biogeochemical significance; contributions to environment.<br><br />
If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.<br><br><br />
<br />
==References==<br />
[1] [http://ijs.sgmjournals.org/cgi/content/abstract/53/1/331 Fernandez-Martinez, Javier, Maria J. Pujalte, Jesus Garcia-Martinez, Manuel Mata, Esperanza Garay, and Francisco Rodriguez-Valera. "Description of ''Alcanivorax Venustensis'' sp. nov. and Reclassification of ''Fundibacter Jadensis'' DSM 12178T (Bruns and Berthe-Corti 1999) As ''Alcanivorax Jadensis'' comb. nov., Members of the Emended Genus ''Alcanivorax''." International Journal of Systematic and Evolutionary Microbiology 53 (2003): 331-338.]<br />
<br />
[2] [http://www.blackwell-synergy.com/doi/pdf/10.1046/j.1468-2920.2003.00468.x Hara, Akihiro, Kazuaki Syutsubo, and Shigeaki Harayama. "''Alcanivorax'' Which Prevails In Oil-contaminated Seawater Exhibits Broad Substrate Specificity For Alkane Degradation." Environmental Microbiology 5.9 (2003): 746-753.]<br />
<br />
[3] [http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html Lorenzo, Víctor De. "Blueprint of an Oil-eating Bacterium." Nature Biotechnology 24 (2006): 952-953.]<br />
<br />
[4] [http://www.ncbi.nlm.nih.gov/pubmed/16878126 Schneiker, S. et al. "Genome Sequence of the Ubiquitous Hydrocarbon-degrading Marine Bacterium A''lcanivorax Borkumensis''." Nature Biotechnology 24 (2006): 997-1004.]<br />
<br />
[5] [http://ijs.sgmjournals.org/cgi/content/abstract/48/2/339 Yakimov, Michail M., Peter N. Golyshin, Siegmund Lang, Edward R. B. Moore, Wolf-Rainer Abraham, Heinrich Lunsdorf, and Kenneth N. Timmis. "''Alcanivorax Borkumensis'' gen. nov., sp. nov., A New, Hydrocarbon-degrading And Surfactant-producing Marine Bacterium." International Journal of Systematic Bacteriology 48 (1998): 339-348.]<br />
<br />
==Author==<br />
Page authored by Andrew Buss, student of [http://www.kbs.msu.edu/faculty/lennon/ Prof. Jay Lennon] at Michigan State University.</div>Bussand1https://microbewiki.kenyon.edu/index.php?title=Alcanivorax&diff=29740Alcanivorax2008-04-06T20:03:34Z<p>Bussand1: /* Cell Structure, Metabolism and Life Cycle */</p>
<hr />
<div>[[Image:AlcVorax PROCARYOTES01b-1.JPG|thumbnail|300px|Figure 1. ''Alcanivorax borkumensis''. Image from Helmholtz Centre for Infection Research[http://www.helmholtz-hzi.de/en/news_public_relation/press_releases/view/article/complete/oil_tanker_accidents_as_a_source_of_food/]]]<br />
<br />
==Classification==<br />
<br />
'''Bacteria'''; Phylum: '''Proteobacteria'''; Class: '''Gammaproteobacteria'''; Order: '''Oceanospirillales'''; Family: '''Alcanivoracaceae'''<br />
<br />
===Species===<br />
<br />
{|<br />
| height="10" bgcolor="#FFDF95" |<br />
'''NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Tree&id=2&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy]'''<br />
|}<br />
<br />
*''Alcanivorax balearicum''<br />
*''Alcanivorax borkumensis''<br />
*''Alcanivorax dieselolei''<br />
*''Alcanivorax indicus''<br />
*''Alcanivorax jadensis''<br />
*''Alcanivorax venustensis''<br />
<br />
==Description and Significance==<br />
[[Image:PWS tanker oil spill.jpg|thumbnail|200px|Figure 2. Supertanker Exxon Valdez grounded on Bligh Reef which released 11 million gallons of crude oil into the water. This oil-contaminated seawater is the preferred habitat for ''Alcanivorax''. Image from USGS[http://menlocampus.wr.usgs.gov/50years/accomplishments/oil.html]]]<br />
<br />
''Alcanivorax'', first described in 1998, is a Gram-negative, halophilic, aerobic, rod-shaped, oil-degrading marine bacterium that is found in low abundances in unpolluted environments in the upper layers of the ocean, but quickly becomes the predominant microbe in oil-contaminated open oceans and coastal waters when nitrogen and phosphorus are not limiting (Hara, 2003). When conditions in these moderately halophilic environments are right, ''Alcanivorax'' may make up 80-90% of the oil-degrading microbes present in the area (Schneiker et al., 2006). It is described as a non-motile bactertium which is true for species such as ''Alcanivorax borkumensis'', but other species such as ''Alcanivorax venustensis'' were described to be motile by polar flagella (Fernandez-Martinez et al., 2003). <br />
<br />
As a result of their profound ability to degrade and live predominately on alkanes, as well as to become the dominant microbes in oil-contaminated areas, ''Alcanivorax'' plays a huge role in the biological cleanup of oil-contaminated environments. These oil-contaminated environments in the ocean are largely due to anthropogenic sources such as oil spills caused by tankers accidents, and cause serious ecological damage to coastal fauna and flora as well as other inhabitants of the ocean. Microbes such as ''Alcanivorax'' provide a major route for the breakdown of these pollutants, and demonstrate how marine bacteria keep the environment in check. Of all the ''Alcanivorax'' species and other oil-degrading microbes, ''Alcanivorax borkumensis'' is one of the most important worldwide due to the fact it produces a wide variety of very efficient oil-degrading enzymes. With this knowledge, ''A. borkumensis'' could provide a useful tool for bioremediation of oil spills.<br />
<br />
==Genome Structure==<br />
[[Image:Lorenzo.gif|thumbnail|200px|Figure 3. Mechanisms for oil degradation and survival encoded by the ''A. borkumensis SK2'' genome. Image from Victor de Lorenzo[http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html]]]<br />
<br />
The ''Alcanivorax borkumensis'' strain SK2, isolated from a seawater sediment sample in the North Sea at a site located near the Isle of Borkum, was the first hydrocarbonoclastic bacterium to be sequenced and was completed by Susanne Schneiker et al. It's genome consists of a single circular chromosome with 3,120,143 base pairs with an average G+C content of 54.7%. The genomic analysis of ''A. borkumensis SK2'' revealed several new insights into the bacterium's role for (i) n-alkane degradation (which includes metabolism, biosurfactant production and biofilm production), (ii) it's system for capturing or scavenging the small amounts of nitrogen, phosphorous, sulfur, and other elements in a nutrient-poor marine environment which allows for more efficient alkane degradation due to their main limitation of nutrient availability, (iii) as well as means for coping with stress factors such as high salt contents and high UV radiation since it thrives mostly in the upper layers in the ocean where UV light is encountered. It's genome encodes several systems for the catabolism of hydrocarbons which allow the bacertium to degrade all sorts of alkanes such as AlkB1 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C5-C12, and AlkB2 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C8 to C16. Both these systems are located close to the origin of replication of the chromosome. ''A. borkumensis'' is also able to degrade alkanes up to C32, branched aliphatic hydrocarbons, isoprenoid hydrocarbons such as phytane, as well as alkylarenes and alkylcycloalkanes. Thus, the genome encodes for a broad spectrum of systems for the catabolism of hydrocarbons, giving it a competitive advantage over other oil-degrading marine microbial communities. To deal with the damaging effects of UV light, ''A. borkumensis'' has a number of genes that reduce the damage. These include the full genes for DNA alkylation, recombinational and nucleotide excision repair, base excision repair, as well as the SOS response (Schneiker et al., 2006).<br />
<br />
==Cell Structure, Metabolism and Life Cycle==<br />
Interesting features of cell structure; how it gains energy; what important molecules it produces.<br />
<br />
Alcanivorax borkumensis is able to use n-alkanes as its principle carbon and energy source by use of the broad spectrum of oil-degrading enzymes it possesses, but they can also use a limited number of organic compounds such as aliphatic hydrocarbons, volatile fatty acids, and pyruvate. However, it cannot utilize carbon sources such as sugars or amino acids. When the slow growing ''A. borkumensis'' uses n-alkanes exclusively, the microbes produce extracellular and membrane-bound surface-active glucose lipids called biosurfactants. These biosurfactants act as natural emulsifiers which enhances the break up of oil-in-water emulsions which prevent degradation of alkanes (Schneiker et al., 2006). Due to the low solubility of oil in water, most oil degradation takes place at the oil-water interface as depicted in figure 2.<br />
<br />
==Ecology and Pathogenesis==<br />
Habitat; symbiosis; biogeochemical significance; contributions to environment.<br><br />
If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.<br><br><br />
<br />
==References==<br />
[1] [http://ijs.sgmjournals.org/cgi/content/abstract/53/1/331 Fernandez-Martinez, Javier, Maria J. Pujalte, Jesus Garcia-Martinez, Manuel Mata, Esperanza Garay, and Francisco Rodriguez-Valera. "Description of ''Alcanivorax Venustensis'' sp. nov. and Reclassification of ''Fundibacter Jadensis'' DSM 12178T (Bruns and Berthe-Corti 1999) As ''Alcanivorax Jadensis'' comb. nov., Members of the Emended Genus ''Alcanivorax''." International Journal of Systematic and Evolutionary Microbiology 53 (2003): 331-338.]<br />
<br />
[2] [http://www.blackwell-synergy.com/doi/pdf/10.1046/j.1468-2920.2003.00468.x Hara, Akihiro, Kazuaki Syutsubo, and Shigeaki Harayama. "''Alcanivorax'' Which Prevails In Oil-contaminated Seawater Exhibits Broad Substrate Specificity For Alkane Degradation." Environmental Microbiology 5.9 (2003): 746-753.]<br />
<br />
[3] [http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html Lorenzo, Víctor De. "Blueprint of an Oil-eating Bacterium." Nature Biotechnology 24 (2006): 952-953.]<br />
<br />
[4] [http://www.ncbi.nlm.nih.gov/pubmed/16878126 Schneiker, S. et al. "Genome Sequence of the Ubiquitous Hydrocarbon-degrading Marine Bacterium A''lcanivorax Borkumensis''." Nature Biotechnology 24 (2006): 997-1004.]<br />
<br />
[5] [http://ijs.sgmjournals.org/cgi/content/abstract/48/2/339 Yakimov, Michail M., Peter N. Golyshin, Siegmund Lang, Edward R. B. Moore, Wolf-Rainer Abraham, Heinrich Lunsdorf, and Kenneth N. Timmis. "''Alcanivorax Borkumensis'' gen. nov., sp. nov., A New, Hydrocarbon-degrading And Surfactant-producing Marine Bacterium." International Journal of Systematic Bacteriology 48 (1998): 339-348.]<br />
<br />
==Author==<br />
Page authored by Andrew Buss, student of [http://www.kbs.msu.edu/faculty/lennon/ Prof. Jay Lennon] at Michigan State University.</div>Bussand1https://microbewiki.kenyon.edu/index.php?title=Alcanivorax&diff=29739Alcanivorax2008-04-06T20:00:05Z<p>Bussand1: /* Cell Structure, Metabolism and Life Cycle */</p>
<hr />
<div>[[Image:AlcVorax PROCARYOTES01b-1.JPG|thumbnail|300px|Figure 1. ''Alcanivorax borkumensis''. Image from Helmholtz Centre for Infection Research[http://www.helmholtz-hzi.de/en/news_public_relation/press_releases/view/article/complete/oil_tanker_accidents_as_a_source_of_food/]]]<br />
<br />
==Classification==<br />
<br />
'''Bacteria'''; Phylum: '''Proteobacteria'''; Class: '''Gammaproteobacteria'''; Order: '''Oceanospirillales'''; Family: '''Alcanivoracaceae'''<br />
<br />
===Species===<br />
<br />
{|<br />
| height="10" bgcolor="#FFDF95" |<br />
'''NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Tree&id=2&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy]'''<br />
|}<br />
<br />
*''Alcanivorax balearicum''<br />
*''Alcanivorax borkumensis''<br />
*''Alcanivorax dieselolei''<br />
*''Alcanivorax indicus''<br />
*''Alcanivorax jadensis''<br />
*''Alcanivorax venustensis''<br />
<br />
==Description and Significance==<br />
[[Image:PWS tanker oil spill.jpg|thumbnail|200px|Figure 2. Supertanker Exxon Valdez grounded on Bligh Reef which released 11 million gallons of crude oil into the water. This oil-contaminated seawater is the preferred habitat for ''Alcanivorax''. Image from USGS[http://menlocampus.wr.usgs.gov/50years/accomplishments/oil.html]]]<br />
<br />
''Alcanivorax'', first described in 1998, is a Gram-negative, halophilic, aerobic, rod-shaped, oil-degrading marine bacterium that is found in low abundances in unpolluted environments in the upper layers of the ocean, but quickly becomes the predominant microbe in oil-contaminated open oceans and coastal waters when nitrogen and phosphorus are not limiting (Hara, 2003). When conditions in these moderately halophilic environments are right, ''Alcanivorax'' may make up 80-90% of the oil-degrading microbes present in the area (Schneiker et al., 2006). It is described as a non-motile bactertium which is true for species such as ''Alcanivorax borkumensis'', but other species such as ''Alcanivorax venustensis'' were described to be motile by polar flagella (Fernandez-Martinez et al., 2003). <br />
<br />
As a result of their profound ability to degrade and live predominately on alkanes, as well as to become the dominant microbes in oil-contaminated areas, ''Alcanivorax'' plays a huge role in the biological cleanup of oil-contaminated environments. These oil-contaminated environments in the ocean are largely due to anthropogenic sources such as oil spills caused by tankers accidents, and cause serious ecological damage to coastal fauna and flora as well as other inhabitants of the ocean. Microbes such as ''Alcanivorax'' provide a major route for the breakdown of these pollutants, and demonstrate how marine bacteria keep the environment in check. Of all the ''Alcanivorax'' species and other oil-degrading microbes, ''Alcanivorax borkumensis'' is one of the most important worldwide due to the fact it produces a wide variety of very efficient oil-degrading enzymes. With this knowledge, ''A. borkumensis'' could provide a useful tool for bioremediation of oil spills.<br />
<br />
==Genome Structure==<br />
[[Image:Lorenzo.gif|thumbnail|200px|Figure 3. Mechanisms for oil degradation and survival encoded by the ''A. borkumensis SK2'' genome. Image from Victor de Lorenzo[http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html]]]<br />
<br />
The ''Alcanivorax borkumensis'' strain SK2, isolated from a seawater sediment sample in the North Sea at a site located near the Isle of Borkum, was the first hydrocarbonoclastic bacterium to be sequenced and was completed by Susanne Schneiker et al. It's genome consists of a single circular chromosome with 3,120,143 base pairs with an average G+C content of 54.7%. The genomic analysis of ''A. borkumensis SK2'' revealed several new insights into the bacterium's role for (i) n-alkane degradation (which includes metabolism, biosurfactant production and biofilm production), (ii) it's system for capturing or scavenging the small amounts of nitrogen, phosphorous, sulfur, and other elements in a nutrient-poor marine environment which allows for more efficient alkane degradation due to their main limitation of nutrient availability, (iii) as well as means for coping with stress factors such as high salt contents and high UV radiation since it thrives mostly in the upper layers in the ocean where UV light is encountered. It's genome encodes several systems for the catabolism of hydrocarbons which allow the bacertium to degrade all sorts of alkanes such as AlkB1 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C5-C12, and AlkB2 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C8 to C16. Both these systems are located close to the origin of replication of the chromosome. ''A. borkumensis'' is also able to degrade alkanes up to C32, branched aliphatic hydrocarbons, isoprenoid hydrocarbons such as phytane, as well as alkylarenes and alkylcycloalkanes. Thus, the genome encodes for a broad spectrum of systems for the catabolism of hydrocarbons, giving it a competitive advantage over other oil-degrading marine microbial communities. To deal with the damaging effects of UV light, ''A. borkumensis'' has a number of genes that reduce the damage. These include the full genes for DNA alkylation, recombinational and nucleotide excision repair, base excision repair, as well as the SOS response (Schneiker et al., 2006).<br />
<br />
==Cell Structure, Metabolism and Life Cycle==<br />
Interesting features of cell structure; how it gains energy; what important molecules it produces.<br />
<br />
Alcanivorax borkumensis is able to use n-alkanes as its principle carbon and energy source by use of the broad spectrum of oil-degrading enzymes it possesses, but they can also use a limited number of organic compounds such as aliphatic hydrocarbons, volatile fatty acids, and pyruvate. However, it cannot utilize carbon sources such as sugars or amino acids. When the slow growing ''A. borkumensis'' uses n-alkanes exclusively, the microbes produce extracellular and membrane-bound surface-active glucose lipids called biosurfactants. These biosurfactants act as natural emulsifiers which enhances the break up of oil-in-water emulsions which prevent degradation of alkanes (Schneiker et al., 2006). Due to the low solubility of oil in water,<br />
<br />
==Ecology and Pathogenesis==<br />
Habitat; symbiosis; biogeochemical significance; contributions to environment.<br><br />
If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.<br><br><br />
<br />
==References==<br />
[1] [http://ijs.sgmjournals.org/cgi/content/abstract/53/1/331 Fernandez-Martinez, Javier, Maria J. Pujalte, Jesus Garcia-Martinez, Manuel Mata, Esperanza Garay, and Francisco Rodriguez-Valera. "Description of ''Alcanivorax Venustensis'' sp. nov. and Reclassification of ''Fundibacter Jadensis'' DSM 12178T (Bruns and Berthe-Corti 1999) As ''Alcanivorax Jadensis'' comb. nov., Members of the Emended Genus ''Alcanivorax''." International Journal of Systematic and Evolutionary Microbiology 53 (2003): 331-338.]<br />
<br />
[2] [http://www.blackwell-synergy.com/doi/pdf/10.1046/j.1468-2920.2003.00468.x Hara, Akihiro, Kazuaki Syutsubo, and Shigeaki Harayama. "''Alcanivorax'' Which Prevails In Oil-contaminated Seawater Exhibits Broad Substrate Specificity For Alkane Degradation." Environmental Microbiology 5.9 (2003): 746-753.]<br />
<br />
[3] [http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html Lorenzo, Víctor De. "Blueprint of an Oil-eating Bacterium." Nature Biotechnology 24 (2006): 952-953.]<br />
<br />
[4] [http://www.ncbi.nlm.nih.gov/pubmed/16878126 Schneiker, S. et al. "Genome Sequence of the Ubiquitous Hydrocarbon-degrading Marine Bacterium A''lcanivorax Borkumensis''." Nature Biotechnology 24 (2006): 997-1004.]<br />
<br />
[5] [http://ijs.sgmjournals.org/cgi/content/abstract/48/2/339 Yakimov, Michail M., Peter N. Golyshin, Siegmund Lang, Edward R. B. Moore, Wolf-Rainer Abraham, Heinrich Lunsdorf, and Kenneth N. Timmis. "''Alcanivorax Borkumensis'' gen. nov., sp. nov., A New, Hydrocarbon-degrading And Surfactant-producing Marine Bacterium." International Journal of Systematic Bacteriology 48 (1998): 339-348.]<br />
<br />
==Author==<br />
Page authored by Andrew Buss, student of [http://www.kbs.msu.edu/faculty/lennon/ Prof. Jay Lennon] at Michigan State University.</div>Bussand1https://microbewiki.kenyon.edu/index.php?title=Alcanivorax&diff=29736Alcanivorax2008-04-06T19:34:09Z<p>Bussand1: /* Genome Structure */</p>
<hr />
<div>[[Image:AlcVorax PROCARYOTES01b-1.JPG|thumbnail|300px|Figure 1. ''Alcanivorax borkumensis''. Image from Helmholtz Centre for Infection Research[http://www.helmholtz-hzi.de/en/news_public_relation/press_releases/view/article/complete/oil_tanker_accidents_as_a_source_of_food/]]]<br />
<br />
==Classification==<br />
<br />
'''Bacteria'''; Phylum: '''Proteobacteria'''; Class: '''Gammaproteobacteria'''; Order: '''Oceanospirillales'''; Family: '''Alcanivoracaceae'''<br />
<br />
===Species===<br />
<br />
{|<br />
| height="10" bgcolor="#FFDF95" |<br />
'''NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Tree&id=2&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy]'''<br />
|}<br />
<br />
*''Alcanivorax balearicum''<br />
*''Alcanivorax borkumensis''<br />
*''Alcanivorax dieselolei''<br />
*''Alcanivorax indicus''<br />
*''Alcanivorax jadensis''<br />
*''Alcanivorax venustensis''<br />
<br />
==Description and Significance==<br />
[[Image:PWS tanker oil spill.jpg|thumbnail|200px|Figure 2. Supertanker Exxon Valdez grounded on Bligh Reef which released 11 million gallons of crude oil into the water. This oil-contaminated seawater is the preferred habitat for ''Alcanivorax''. Image from USGS[http://menlocampus.wr.usgs.gov/50years/accomplishments/oil.html]]]<br />
<br />
''Alcanivorax'', first described in 1998, is a Gram-negative, halophilic, aerobic, rod-shaped, oil-degrading marine bacterium that is found in low abundances in unpolluted environments in the upper layers of the ocean, but quickly becomes the predominant microbe in oil-contaminated open oceans and coastal waters when nitrogen and phosphorus are not limiting (Hara, 2003). When conditions in these moderately halophilic environments are right, ''Alcanivorax'' may make up 80-90% of the oil-degrading microbes present in the area (Schneiker et al., 2006). It is described as a non-motile bactertium which is true for species such as ''Alcanivorax borkumensis'', but other species such as ''Alcanivorax venustensis'' were described to be motile by polar flagella (Fernandez-Martinez et al., 2003). <br />
<br />
As a result of their profound ability to degrade and live predominately on alkanes, as well as to become the dominant microbes in oil-contaminated areas, ''Alcanivorax'' plays a huge role in the biological cleanup of oil-contaminated environments. These oil-contaminated environments in the ocean are largely due to anthropogenic sources such as oil spills caused by tankers accidents, and cause serious ecological damage to coastal fauna and flora as well as other inhabitants of the ocean. Microbes such as ''Alcanivorax'' provide a major route for the breakdown of these pollutants, and demonstrate how marine bacteria keep the environment in check. Of all the ''Alcanivorax'' species and other oil-degrading microbes, ''Alcanivorax borkumensis'' is one of the most important worldwide due to the fact it produces a wide variety of very efficient oil-degrading enzymes. With this knowledge, ''A. borkumensis'' could provide a useful tool for bioremediation of oil spills.<br />
<br />
==Genome Structure==<br />
[[Image:Lorenzo.gif|thumbnail|200px|Figure 3. Mechanisms for oil degradation and survival encoded by the ''A. borkumensis SK2'' genome. Image from Victor de Lorenzo[http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html]]]<br />
<br />
The ''Alcanivorax borkumensis'' strain SK2, isolated from a seawater sediment sample in the North Sea at a site located near the Isle of Borkum, was the first hydrocarbonoclastic bacterium to be sequenced and was completed by Susanne Schneiker et al. It's genome consists of a single circular chromosome with 3,120,143 base pairs with an average G+C content of 54.7%. The genomic analysis of ''A. borkumensis SK2'' revealed several new insights into the bacterium's role for (i) n-alkane degradation (which includes metabolism, biosurfactant production and biofilm production), (ii) it's system for capturing or scavenging the small amounts of nitrogen, phosphorous, sulfur, and other elements in a nutrient-poor marine environment which allows for more efficient alkane degradation due to their main limitation of nutrient availability, (iii) as well as means for coping with stress factors such as high salt contents and high UV radiation since it thrives mostly in the upper layers in the ocean where UV light is encountered. It's genome encodes several systems for the catabolism of hydrocarbons which allow the bacertium to degrade all sorts of alkanes such as AlkB1 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C5-C12, and AlkB2 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C8 to C16. Both these systems are located close to the origin of replication of the chromosome. ''A. borkumensis'' is also able to degrade alkanes up to C32, branched aliphatic hydrocarbons, isoprenoid hydrocarbons such as phytane, as well as alkylarenes and alkylcycloalkanes. Thus, the genome encodes for a broad spectrum of systems for the catabolism of hydrocarbons, giving it a competitive advantage over other oil-degrading marine microbial communities. To deal with the damaging effects of UV light, ''A. borkumensis'' has a number of genes that reduce the damage. These include the full genes for DNA alkylation, recombinational and nucleotide excision repair, base excision repair, as well as the SOS response (Schneiker et al., 2006).<br />
<br />
==Cell Structure, Metabolism and Life Cycle==<br />
Interesting features of cell structure; how it gains energy; what important molecules it produces.<br />
<br />
Alcanivorax borkumensis is able to use n-alkanes as its principle carbon and energy source by use of the broad spectrum of oil-degrading enzymes it possesses, but they can also use a limited number of organic compounds such as aliphatic hydrocarbons, volatile fatty acids, and pyruvate.<br />
<br />
==Ecology and Pathogenesis==<br />
Habitat; symbiosis; biogeochemical significance; contributions to environment.<br><br />
If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.<br><br><br />
<br />
==References==<br />
[1] [http://ijs.sgmjournals.org/cgi/content/abstract/53/1/331 Fernandez-Martinez, Javier, Maria J. Pujalte, Jesus Garcia-Martinez, Manuel Mata, Esperanza Garay, and Francisco Rodriguez-Valera. "Description of ''Alcanivorax Venustensis'' sp. nov. and Reclassification of ''Fundibacter Jadensis'' DSM 12178T (Bruns and Berthe-Corti 1999) As ''Alcanivorax Jadensis'' comb. nov., Members of the Emended Genus ''Alcanivorax''." International Journal of Systematic and Evolutionary Microbiology 53 (2003): 331-338.]<br />
<br />
[2] [http://www.blackwell-synergy.com/doi/pdf/10.1046/j.1468-2920.2003.00468.x Hara, Akihiro, Kazuaki Syutsubo, and Shigeaki Harayama. "''Alcanivorax'' Which Prevails In Oil-contaminated Seawater Exhibits Broad Substrate Specificity For Alkane Degradation." Environmental Microbiology 5.9 (2003): 746-753.]<br />
<br />
[3] [http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html Lorenzo, Víctor De. "Blueprint of an Oil-eating Bacterium." Nature Biotechnology 24 (2006): 952-953.]<br />
<br />
[4] [http://www.ncbi.nlm.nih.gov/pubmed/16878126 Schneiker, S. et al. "Genome Sequence of the Ubiquitous Hydrocarbon-degrading Marine Bacterium A''lcanivorax Borkumensis''." Nature Biotechnology 24 (2006): 997-1004.]<br />
<br />
[5] [http://ijs.sgmjournals.org/cgi/content/abstract/48/2/339 Yakimov, Michail M., Peter N. Golyshin, Siegmund Lang, Edward R. B. Moore, Wolf-Rainer Abraham, Heinrich Lunsdorf, and Kenneth N. Timmis. "''Alcanivorax Borkumensis'' gen. nov., sp. nov., A New, Hydrocarbon-degrading And Surfactant-producing Marine Bacterium." International Journal of Systematic Bacteriology 48 (1998): 339-348.]<br />
<br />
==Author==<br />
Page authored by Andrew Buss, student of [http://www.kbs.msu.edu/faculty/lennon/ Prof. Jay Lennon] at Michigan State University.</div>Bussand1https://microbewiki.kenyon.edu/index.php?title=Alcanivorax&diff=29734Alcanivorax2008-04-06T19:31:49Z<p>Bussand1: /* Cell Structure, Metabolism and Life Cycle */</p>
<hr />
<div>[[Image:AlcVorax PROCARYOTES01b-1.JPG|thumbnail|300px|Figure 1. ''Alcanivorax borkumensis''. Image from Helmholtz Centre for Infection Research[http://www.helmholtz-hzi.de/en/news_public_relation/press_releases/view/article/complete/oil_tanker_accidents_as_a_source_of_food/]]]<br />
<br />
==Classification==<br />
<br />
'''Bacteria'''; Phylum: '''Proteobacteria'''; Class: '''Gammaproteobacteria'''; Order: '''Oceanospirillales'''; Family: '''Alcanivoracaceae'''<br />
<br />
===Species===<br />
<br />
{|<br />
| height="10" bgcolor="#FFDF95" |<br />
'''NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Tree&id=2&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy]'''<br />
|}<br />
<br />
*''Alcanivorax balearicum''<br />
*''Alcanivorax borkumensis''<br />
*''Alcanivorax dieselolei''<br />
*''Alcanivorax indicus''<br />
*''Alcanivorax jadensis''<br />
*''Alcanivorax venustensis''<br />
<br />
==Description and Significance==<br />
[[Image:PWS tanker oil spill.jpg|thumbnail|200px|Figure 2. Supertanker Exxon Valdez grounded on Bligh Reef which released 11 million gallons of crude oil into the water. This oil-contaminated seawater is the preferred habitat for ''Alcanivorax''. Image from USGS[http://menlocampus.wr.usgs.gov/50years/accomplishments/oil.html]]]<br />
<br />
''Alcanivorax'', first described in 1998, is a Gram-negative, halophilic, aerobic, rod-shaped, oil-degrading marine bacterium that is found in low abundances in unpolluted environments in the upper layers of the ocean, but quickly becomes the predominant microbe in oil-contaminated open oceans and coastal waters when nitrogen and phosphorus are not limiting (Hara, 2003). When conditions in these moderately halophilic environments are right, ''Alcanivorax'' may make up 80-90% of the oil-degrading microbes present in the area (Schneiker et al., 2006). It is described as a non-motile bactertium which is true for species such as ''Alcanivorax borkumensis'', but other species such as ''Alcanivorax venustensis'' were described to be motile by polar flagella (Fernandez-Martinez et al., 2003). <br />
<br />
As a result of their profound ability to degrade and live predominately on alkanes, as well as to become the dominant microbes in oil-contaminated areas, ''Alcanivorax'' plays a huge role in the biological cleanup of oil-contaminated environments. These oil-contaminated environments in the ocean are largely due to anthropogenic sources such as oil spills caused by tankers accidents, and cause serious ecological damage to coastal fauna and flora as well as other inhabitants of the ocean. Microbes such as ''Alcanivorax'' provide a major route for the breakdown of these pollutants, and demonstrate how marine bacteria keep the environment in check. Of all the ''Alcanivorax'' species and other oil-degrading microbes, ''Alcanivorax borkumensis'' is one of the most important worldwide due to the fact it produces a wide variety of very efficient oil-degrading enzymes. With this knowledge, ''A. borkumensis'' could provide a useful tool for bioremediation of oil spills.<br />
<br />
==Genome Structure==<br />
[[Image:Lorenzo.gif|thumbnail|200px|Figure 3. Mechanisms for oil degradation and survival encoded by the ''A. borkumensis SK2'' genome. Image from Victor de Lorenzo[http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html]]]<br />
<br />
The ''Alcanivorax borkumensis'' strain SK2 was the first hydrocarbonoclastic bacterium to be sequenced and was completed by Susanne Schneiker et al. It's genome consists of a single circular chromosome with 3,120,143 base pairs with an average G+C content of 54.7%. The genomic analysis of ''A. borkumensis SK2'' revealed several new insights into the bacterium's role for (i) n-alkane degradation (which includes metabolism, biosurfactant production and biofilm production), (ii) it's system for capturing or scavenging the small amounts of nitrogen, phosphorous, sulfur, and other elements in a nutrient-poor marine environment which allows for more efficient alkane degradation due to their main limitation of nutrient availability, (iii) as well as means for coping with stress factors such as high salt contents and high UV radiation since it thrives mostly in the upper layers in the ocean where UV light is encountered. It's genome encodes several systems for the catabolism of hydrocarbons which allow the bacertium to degrade all sorts of alkanes such as AlkB1 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C5-C12, and AlkB2 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C8 to C16. Both these systems are located close to the origin of replication of the chromosome. ''A. borkumensis'' is also able to degrade alkanes up to C32, branched aliphatic hydrocarbons, isoprenoid hydrocarbons such as phytane, as well as alkylarenes and alkylcycloalkanes. Thus, the genome encodes for a broad spectrum of systems for the catabolism of hydrocarbons, giving it a competitive advantage over other oil-degrading marine microbial communities. To deal with the damaging effects of UV light, ''A. borkumensis'' has a number of genes that reduce the damage. These include the full genes for DNA alkylation, recombinational and nucleotide excision repair, base excision repair, as well as the SOS response (Schneiker et al., 2006).<br />
<br />
==Cell Structure, Metabolism and Life Cycle==<br />
Interesting features of cell structure; how it gains energy; what important molecules it produces.<br />
<br />
Alcanivorax borkumensis is able to use n-alkanes as its principle carbon and energy source by use of the broad spectrum of oil-degrading enzymes it possesses, but they can also use a limited number of organic compounds such as aliphatic hydrocarbons, volatile fatty acids, and pyruvate.<br />
<br />
==Ecology and Pathogenesis==<br />
Habitat; symbiosis; biogeochemical significance; contributions to environment.<br><br />
If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.<br><br><br />
<br />
==References==<br />
[1] [http://ijs.sgmjournals.org/cgi/content/abstract/53/1/331 Fernandez-Martinez, Javier, Maria J. Pujalte, Jesus Garcia-Martinez, Manuel Mata, Esperanza Garay, and Francisco Rodriguez-Valera. "Description of ''Alcanivorax Venustensis'' sp. nov. and Reclassification of ''Fundibacter Jadensis'' DSM 12178T (Bruns and Berthe-Corti 1999) As ''Alcanivorax Jadensis'' comb. nov., Members of the Emended Genus ''Alcanivorax''." International Journal of Systematic and Evolutionary Microbiology 53 (2003): 331-338.]<br />
<br />
[2] [http://www.blackwell-synergy.com/doi/pdf/10.1046/j.1468-2920.2003.00468.x Hara, Akihiro, Kazuaki Syutsubo, and Shigeaki Harayama. "''Alcanivorax'' Which Prevails In Oil-contaminated Seawater Exhibits Broad Substrate Specificity For Alkane Degradation." Environmental Microbiology 5.9 (2003): 746-753.]<br />
<br />
[3] [http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html Lorenzo, Víctor De. "Blueprint of an Oil-eating Bacterium." Nature Biotechnology 24 (2006): 952-953.]<br />
<br />
[4] [http://www.ncbi.nlm.nih.gov/pubmed/16878126 Schneiker, S. et al. "Genome Sequence of the Ubiquitous Hydrocarbon-degrading Marine Bacterium A''lcanivorax Borkumensis''." Nature Biotechnology 24 (2006): 997-1004.]<br />
<br />
[5] [http://ijs.sgmjournals.org/cgi/content/abstract/48/2/339 Yakimov, Michail M., Peter N. Golyshin, Siegmund Lang, Edward R. B. Moore, Wolf-Rainer Abraham, Heinrich Lunsdorf, and Kenneth N. Timmis. "''Alcanivorax Borkumensis'' gen. nov., sp. nov., A New, Hydrocarbon-degrading And Surfactant-producing Marine Bacterium." International Journal of Systematic Bacteriology 48 (1998): 339-348.]<br />
<br />
==Author==<br />
Page authored by Andrew Buss, student of [http://www.kbs.msu.edu/faculty/lennon/ Prof. Jay Lennon] at Michigan State University.</div>Bussand1https://microbewiki.kenyon.edu/index.php?title=Alcanivorax&diff=29733Alcanivorax2008-04-06T19:25:35Z<p>Bussand1: /* Description and Significance */</p>
<hr />
<div>[[Image:AlcVorax PROCARYOTES01b-1.JPG|thumbnail|300px|Figure 1. ''Alcanivorax borkumensis''. Image from Helmholtz Centre for Infection Research[http://www.helmholtz-hzi.de/en/news_public_relation/press_releases/view/article/complete/oil_tanker_accidents_as_a_source_of_food/]]]<br />
<br />
==Classification==<br />
<br />
'''Bacteria'''; Phylum: '''Proteobacteria'''; Class: '''Gammaproteobacteria'''; Order: '''Oceanospirillales'''; Family: '''Alcanivoracaceae'''<br />
<br />
===Species===<br />
<br />
{|<br />
| height="10" bgcolor="#FFDF95" |<br />
'''NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Tree&id=2&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy]'''<br />
|}<br />
<br />
*''Alcanivorax balearicum''<br />
*''Alcanivorax borkumensis''<br />
*''Alcanivorax dieselolei''<br />
*''Alcanivorax indicus''<br />
*''Alcanivorax jadensis''<br />
*''Alcanivorax venustensis''<br />
<br />
==Description and Significance==<br />
[[Image:PWS tanker oil spill.jpg|thumbnail|200px|Figure 2. Supertanker Exxon Valdez grounded on Bligh Reef which released 11 million gallons of crude oil into the water. This oil-contaminated seawater is the preferred habitat for ''Alcanivorax''. Image from USGS[http://menlocampus.wr.usgs.gov/50years/accomplishments/oil.html]]]<br />
<br />
''Alcanivorax'', first described in 1998, is a Gram-negative, halophilic, aerobic, rod-shaped, oil-degrading marine bacterium that is found in low abundances in unpolluted environments in the upper layers of the ocean, but quickly becomes the predominant microbe in oil-contaminated open oceans and coastal waters when nitrogen and phosphorus are not limiting (Hara, 2003). When conditions in these moderately halophilic environments are right, ''Alcanivorax'' may make up 80-90% of the oil-degrading microbes present in the area (Schneiker et al., 2006). It is described as a non-motile bactertium which is true for species such as ''Alcanivorax borkumensis'', but other species such as ''Alcanivorax venustensis'' were described to be motile by polar flagella (Fernandez-Martinez et al., 2003). <br />
<br />
As a result of their profound ability to degrade and live predominately on alkanes, as well as to become the dominant microbes in oil-contaminated areas, ''Alcanivorax'' plays a huge role in the biological cleanup of oil-contaminated environments. These oil-contaminated environments in the ocean are largely due to anthropogenic sources such as oil spills caused by tankers accidents, and cause serious ecological damage to coastal fauna and flora as well as other inhabitants of the ocean. Microbes such as ''Alcanivorax'' provide a major route for the breakdown of these pollutants, and demonstrate how marine bacteria keep the environment in check. Of all the ''Alcanivorax'' species and other oil-degrading microbes, ''Alcanivorax borkumensis'' is one of the most important worldwide due to the fact it produces a wide variety of very efficient oil-degrading enzymes. With this knowledge, ''A. borkumensis'' could provide a useful tool for bioremediation of oil spills.<br />
<br />
==Genome Structure==<br />
[[Image:Lorenzo.gif|thumbnail|200px|Figure 3. Mechanisms for oil degradation and survival encoded by the ''A. borkumensis SK2'' genome. Image from Victor de Lorenzo[http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html]]]<br />
<br />
The ''Alcanivorax borkumensis'' strain SK2 was the first hydrocarbonoclastic bacterium to be sequenced and was completed by Susanne Schneiker et al. It's genome consists of a single circular chromosome with 3,120,143 base pairs with an average G+C content of 54.7%. The genomic analysis of ''A. borkumensis SK2'' revealed several new insights into the bacterium's role for (i) n-alkane degradation (which includes metabolism, biosurfactant production and biofilm production), (ii) it's system for capturing or scavenging the small amounts of nitrogen, phosphorous, sulfur, and other elements in a nutrient-poor marine environment which allows for more efficient alkane degradation due to their main limitation of nutrient availability, (iii) as well as means for coping with stress factors such as high salt contents and high UV radiation since it thrives mostly in the upper layers in the ocean where UV light is encountered. It's genome encodes several systems for the catabolism of hydrocarbons which allow the bacertium to degrade all sorts of alkanes such as AlkB1 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C5-C12, and AlkB2 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C8 to C16. Both these systems are located close to the origin of replication of the chromosome. ''A. borkumensis'' is also able to degrade alkanes up to C32, branched aliphatic hydrocarbons, isoprenoid hydrocarbons such as phytane, as well as alkylarenes and alkylcycloalkanes. Thus, the genome encodes for a broad spectrum of systems for the catabolism of hydrocarbons, giving it a competitive advantage over other oil-degrading marine microbial communities. To deal with the damaging effects of UV light, ''A. borkumensis'' has a number of genes that reduce the damage. These include the full genes for DNA alkylation, recombinational and nucleotide excision repair, base excision repair, as well as the SOS response (Schneiker et al., 2006).<br />
<br />
==Cell Structure, Metabolism and Life Cycle==<br />
Interesting features of cell structure; how it gains energy; what important molecules it produces.<br />
<br />
Alcanivorax borkumensis is able to use n-alkanes as its principle carbon and energy source, but can also use<br />
<br />
==Ecology and Pathogenesis==<br />
Habitat; symbiosis; biogeochemical significance; contributions to environment.<br><br />
If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.<br><br><br />
<br />
==References==<br />
[1] [http://ijs.sgmjournals.org/cgi/content/abstract/53/1/331 Fernandez-Martinez, Javier, Maria J. Pujalte, Jesus Garcia-Martinez, Manuel Mata, Esperanza Garay, and Francisco Rodriguez-Valera. "Description of ''Alcanivorax Venustensis'' sp. nov. and Reclassification of ''Fundibacter Jadensis'' DSM 12178T (Bruns and Berthe-Corti 1999) As ''Alcanivorax Jadensis'' comb. nov., Members of the Emended Genus ''Alcanivorax''." International Journal of Systematic and Evolutionary Microbiology 53 (2003): 331-338.]<br />
<br />
[2] [http://www.blackwell-synergy.com/doi/pdf/10.1046/j.1468-2920.2003.00468.x Hara, Akihiro, Kazuaki Syutsubo, and Shigeaki Harayama. "''Alcanivorax'' Which Prevails In Oil-contaminated Seawater Exhibits Broad Substrate Specificity For Alkane Degradation." Environmental Microbiology 5.9 (2003): 746-753.]<br />
<br />
[3] [http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html Lorenzo, Víctor De. "Blueprint of an Oil-eating Bacterium." Nature Biotechnology 24 (2006): 952-953.]<br />
<br />
[4] [http://www.ncbi.nlm.nih.gov/pubmed/16878126 Schneiker, S. et al. "Genome Sequence of the Ubiquitous Hydrocarbon-degrading Marine Bacterium A''lcanivorax Borkumensis''." Nature Biotechnology 24 (2006): 997-1004.]<br />
<br />
[5] [http://ijs.sgmjournals.org/cgi/content/abstract/48/2/339 Yakimov, Michail M., Peter N. Golyshin, Siegmund Lang, Edward R. B. Moore, Wolf-Rainer Abraham, Heinrich Lunsdorf, and Kenneth N. Timmis. "''Alcanivorax Borkumensis'' gen. nov., sp. nov., A New, Hydrocarbon-degrading And Surfactant-producing Marine Bacterium." International Journal of Systematic Bacteriology 48 (1998): 339-348.]<br />
<br />
==Author==<br />
Page authored by Andrew Buss, student of [http://www.kbs.msu.edu/faculty/lennon/ Prof. Jay Lennon] at Michigan State University.</div>Bussand1https://microbewiki.kenyon.edu/index.php?title=Alcanivorax&diff=29732Alcanivorax2008-04-06T19:21:38Z<p>Bussand1: /* Cell Structure, Metabolism and Life Cycle */</p>
<hr />
<div>[[Image:AlcVorax PROCARYOTES01b-1.JPG|thumbnail|300px|Figure 1. ''Alcanivorax borkumensis''. Image from Helmholtz Centre for Infection Research[http://www.helmholtz-hzi.de/en/news_public_relation/press_releases/view/article/complete/oil_tanker_accidents_as_a_source_of_food/]]]<br />
<br />
==Classification==<br />
<br />
'''Bacteria'''; Phylum: '''Proteobacteria'''; Class: '''Gammaproteobacteria'''; Order: '''Oceanospirillales'''; Family: '''Alcanivoracaceae'''<br />
<br />
===Species===<br />
<br />
{|<br />
| height="10" bgcolor="#FFDF95" |<br />
'''NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Tree&id=2&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy]'''<br />
|}<br />
<br />
*''Alcanivorax balearicum''<br />
*''Alcanivorax borkumensis''<br />
*''Alcanivorax dieselolei''<br />
*''Alcanivorax indicus''<br />
*''Alcanivorax jadensis''<br />
*''Alcanivorax venustensis''<br />
<br />
==Description and Significance==<br />
[[Image:PWS tanker oil spill.jpg|thumbnail|200px|Figure 2. Supertanker Exxon Valdez grounded on Bligh Reef which released 11 million gallons of crude oil into the water. This oil-contaminated seawater is the preferred habitat for ''Alcanivorax''. Image from USGS[http://menlocampus.wr.usgs.gov/50years/accomplishments/oil.html]]]<br />
<br />
''Alcanivorax'', first described in 1998, is a Gram-negative, halophilic, aerobic, rod-shaped, oil-degrading marine bacterium that is found in low abundances in unpolluted environments in the upper layers of the ocean, but quickly becomes the predominant microbes in oil-contaminated open oceans and coastal waters when nitrogen and phosphorus are not limiting (Hara, 2003). When conditions in these moderately halophilic environments are right, ''Alcanivorax'' may make up 80-90% of the oil-degrading microbial community. It is described as a non-motile bactertium which is true for species such as ''Alcanivorax borkumensis'', but other species such as ''Alcanivorax venustensis'' were described to be motile by polar flagella (Fernandez-Martinez et al., 2003). <br />
<br />
As a result of their profound ability to degrade and live predominately on alkanes, as well as to become the dominant microbes in oil-contaminated areas, ''Alcanivorax'' plays a huge role in the biological cleanup of oil-contaminated environments. These oil-contaminated environments in the ocean are largely due to anthropogenic sources such as oil spills caused by tankers accidents, and cause serious ecological damage to coastal fauna and flora as well as other inhabitants of the ocean. Microbes such as ''Alcanivorax'' provide a major route for the breakdown of these pollutants, and demonstrate how marine bacteria keep the environment in check. Of all the ''Alcanivorax'' species and other oil-degrading microbes, ''Alcanivorax borkumensis'' is one of the most important worldwide due to the fact it produces a wide variety of very efficient oil-degrading enzymes. With this knowledge, ''A. borkumensis'' could provide a useful tool for bioremediation of oil spills.<br />
<br />
==Genome Structure==<br />
[[Image:Lorenzo.gif|thumbnail|200px|Figure 3. Mechanisms for oil degradation and survival encoded by the ''A. borkumensis SK2'' genome. Image from Victor de Lorenzo[http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html]]]<br />
<br />
The ''Alcanivorax borkumensis'' strain SK2 was the first hydrocarbonoclastic bacterium to be sequenced and was completed by Susanne Schneiker et al. It's genome consists of a single circular chromosome with 3,120,143 base pairs with an average G+C content of 54.7%. The genomic analysis of ''A. borkumensis SK2'' revealed several new insights into the bacterium's role for (i) n-alkane degradation (which includes metabolism, biosurfactant production and biofilm production), (ii) it's system for capturing or scavenging the small amounts of nitrogen, phosphorous, sulfur, and other elements in a nutrient-poor marine environment which allows for more efficient alkane degradation due to their main limitation of nutrient availability, (iii) as well as means for coping with stress factors such as high salt contents and high UV radiation since it thrives mostly in the upper layers in the ocean where UV light is encountered. It's genome encodes several systems for the catabolism of hydrocarbons which allow the bacertium to degrade all sorts of alkanes such as AlkB1 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C5-C12, and AlkB2 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C8 to C16. Both these systems are located close to the origin of replication of the chromosome. ''A. borkumensis'' is also able to degrade alkanes up to C32, branched aliphatic hydrocarbons, isoprenoid hydrocarbons such as phytane, as well as alkylarenes and alkylcycloalkanes. Thus, the genome encodes for a broad spectrum of systems for the catabolism of hydrocarbons, giving it a competitive advantage over other oil-degrading marine microbial communities. To deal with the damaging effects of UV light, ''A. borkumensis'' has a number of genes that reduce the damage. These include the full genes for DNA alkylation, recombinational and nucleotide excision repair, base excision repair, as well as the SOS response (Schneiker et al., 2006).<br />
<br />
==Cell Structure, Metabolism and Life Cycle==<br />
Interesting features of cell structure; how it gains energy; what important molecules it produces.<br />
<br />
Alcanivorax borkumensis is able to use n-alkanes as its principle carbon and energy source, but can also use<br />
<br />
==Ecology and Pathogenesis==<br />
Habitat; symbiosis; biogeochemical significance; contributions to environment.<br><br />
If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.<br><br><br />
<br />
==References==<br />
[1] [http://ijs.sgmjournals.org/cgi/content/abstract/53/1/331 Fernandez-Martinez, Javier, Maria J. Pujalte, Jesus Garcia-Martinez, Manuel Mata, Esperanza Garay, and Francisco Rodriguez-Valera. "Description of ''Alcanivorax Venustensis'' sp. nov. and Reclassification of ''Fundibacter Jadensis'' DSM 12178T (Bruns and Berthe-Corti 1999) As ''Alcanivorax Jadensis'' comb. nov., Members of the Emended Genus ''Alcanivorax''." International Journal of Systematic and Evolutionary Microbiology 53 (2003): 331-338.]<br />
<br />
[2] [http://www.blackwell-synergy.com/doi/pdf/10.1046/j.1468-2920.2003.00468.x Hara, Akihiro, Kazuaki Syutsubo, and Shigeaki Harayama. "''Alcanivorax'' Which Prevails In Oil-contaminated Seawater Exhibits Broad Substrate Specificity For Alkane Degradation." Environmental Microbiology 5.9 (2003): 746-753.]<br />
<br />
[3] [http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html Lorenzo, Víctor De. "Blueprint of an Oil-eating Bacterium." Nature Biotechnology 24 (2006): 952-953.]<br />
<br />
[4] [http://www.ncbi.nlm.nih.gov/pubmed/16878126 Schneiker, S. et al. "Genome Sequence of the Ubiquitous Hydrocarbon-degrading Marine Bacterium A''lcanivorax Borkumensis''." Nature Biotechnology 24 (2006): 997-1004.]<br />
<br />
[5] [http://ijs.sgmjournals.org/cgi/content/abstract/48/2/339 Yakimov, Michail M., Peter N. Golyshin, Siegmund Lang, Edward R. B. Moore, Wolf-Rainer Abraham, Heinrich Lunsdorf, and Kenneth N. Timmis. "''Alcanivorax Borkumensis'' gen. nov., sp. nov., A New, Hydrocarbon-degrading And Surfactant-producing Marine Bacterium." International Journal of Systematic Bacteriology 48 (1998): 339-348.]<br />
<br />
==Author==<br />
Page authored by Andrew Buss, student of [http://www.kbs.msu.edu/faculty/lennon/ Prof. Jay Lennon] at Michigan State University.</div>Bussand1https://microbewiki.kenyon.edu/index.php?title=Alcanivorax&diff=29731Alcanivorax2008-04-06T19:01:19Z<p>Bussand1: /* Description and Significance */</p>
<hr />
<div>[[Image:AlcVorax PROCARYOTES01b-1.JPG|thumbnail|300px|Figure 1. ''Alcanivorax borkumensis''. Image from Helmholtz Centre for Infection Research[http://www.helmholtz-hzi.de/en/news_public_relation/press_releases/view/article/complete/oil_tanker_accidents_as_a_source_of_food/]]]<br />
<br />
==Classification==<br />
<br />
'''Bacteria'''; Phylum: '''Proteobacteria'''; Class: '''Gammaproteobacteria'''; Order: '''Oceanospirillales'''; Family: '''Alcanivoracaceae'''<br />
<br />
===Species===<br />
<br />
{|<br />
| height="10" bgcolor="#FFDF95" |<br />
'''NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Tree&id=2&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy]'''<br />
|}<br />
<br />
*''Alcanivorax balearicum''<br />
*''Alcanivorax borkumensis''<br />
*''Alcanivorax dieselolei''<br />
*''Alcanivorax indicus''<br />
*''Alcanivorax jadensis''<br />
*''Alcanivorax venustensis''<br />
<br />
==Description and Significance==<br />
[[Image:PWS tanker oil spill.jpg|thumbnail|200px|Figure 2. Supertanker Exxon Valdez grounded on Bligh Reef which released 11 million gallons of crude oil into the water. This oil-contaminated seawater is the preferred habitat for ''Alcanivorax''. Image from USGS[http://menlocampus.wr.usgs.gov/50years/accomplishments/oil.html]]]<br />
<br />
''Alcanivorax'', first described in 1998, is a Gram-negative, halophilic, aerobic, rod-shaped, oil-degrading marine bacterium that is found in low abundances in unpolluted environments in the upper layers of the ocean, but quickly becomes the predominant microbes in oil-contaminated open oceans and coastal waters when nitrogen and phosphorus are not limiting (Hara, 2003). When conditions in these moderately halophilic environments are right, ''Alcanivorax'' may make up 80-90% of the oil-degrading microbial community. It is described as a non-motile bactertium which is true for species such as ''Alcanivorax borkumensis'', but other species such as ''Alcanivorax venustensis'' were described to be motile by polar flagella (Fernandez-Martinez et al., 2003). <br />
<br />
As a result of their profound ability to degrade and live predominately on alkanes, as well as to become the dominant microbes in oil-contaminated areas, ''Alcanivorax'' plays a huge role in the biological cleanup of oil-contaminated environments. These oil-contaminated environments in the ocean are largely due to anthropogenic sources such as oil spills caused by tankers accidents, and cause serious ecological damage to coastal fauna and flora as well as other inhabitants of the ocean. Microbes such as ''Alcanivorax'' provide a major route for the breakdown of these pollutants, and demonstrate how marine bacteria keep the environment in check. Of all the ''Alcanivorax'' species and other oil-degrading microbes, ''Alcanivorax borkumensis'' is one of the most important worldwide due to the fact it produces a wide variety of very efficient oil-degrading enzymes. With this knowledge, ''A. borkumensis'' could provide a useful tool for bioremediation of oil spills.<br />
<br />
==Genome Structure==<br />
[[Image:Lorenzo.gif|thumbnail|200px|Figure 3. Mechanisms for oil degradation and survival encoded by the ''A. borkumensis SK2'' genome. Image from Victor de Lorenzo[http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html]]]<br />
<br />
The ''Alcanivorax borkumensis'' strain SK2 was the first hydrocarbonoclastic bacterium to be sequenced and was completed by Susanne Schneiker et al. It's genome consists of a single circular chromosome with 3,120,143 base pairs with an average G+C content of 54.7%. The genomic analysis of ''A. borkumensis SK2'' revealed several new insights into the bacterium's role for (i) n-alkane degradation (which includes metabolism, biosurfactant production and biofilm production), (ii) it's system for capturing or scavenging the small amounts of nitrogen, phosphorous, sulfur, and other elements in a nutrient-poor marine environment which allows for more efficient alkane degradation due to their main limitation of nutrient availability, (iii) as well as means for coping with stress factors such as high salt contents and high UV radiation since it thrives mostly in the upper layers in the ocean where UV light is encountered. It's genome encodes several systems for the catabolism of hydrocarbons which allow the bacertium to degrade all sorts of alkanes such as AlkB1 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C5-C12, and AlkB2 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C8 to C16. Both these systems are located close to the origin of replication of the chromosome. ''A. borkumensis'' is also able to degrade alkanes up to C32, branched aliphatic hydrocarbons, isoprenoid hydrocarbons such as phytane, as well as alkylarenes and alkylcycloalkanes. Thus, the genome encodes for a broad spectrum of systems for the catabolism of hydrocarbons, giving it a competitive advantage over other oil-degrading marine microbial communities. To deal with the damaging effects of UV light, ''A. borkumensis'' has a number of genes that reduce the damage. These include the full genes for DNA alkylation, recombinational and nucleotide excision repair, base excision repair, as well as the SOS response (Schneiker et al., 2006).<br />
<br />
==Cell Structure, Metabolism and Life Cycle==<br />
Interesting features of cell structure; how it gains energy; what important molecules it produces.<br />
<br />
<br />
==Ecology and Pathogenesis==<br />
Habitat; symbiosis; biogeochemical significance; contributions to environment.<br><br />
If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.<br><br><br />
<br />
==References==<br />
[1] [http://ijs.sgmjournals.org/cgi/content/abstract/53/1/331 Fernandez-Martinez, Javier, Maria J. Pujalte, Jesus Garcia-Martinez, Manuel Mata, Esperanza Garay, and Francisco Rodriguez-Valera. "Description of ''Alcanivorax Venustensis'' sp. nov. and Reclassification of ''Fundibacter Jadensis'' DSM 12178T (Bruns and Berthe-Corti 1999) As ''Alcanivorax Jadensis'' comb. nov., Members of the Emended Genus ''Alcanivorax''." International Journal of Systematic and Evolutionary Microbiology 53 (2003): 331-338.]<br />
<br />
[2] [http://www.blackwell-synergy.com/doi/pdf/10.1046/j.1468-2920.2003.00468.x Hara, Akihiro, Kazuaki Syutsubo, and Shigeaki Harayama. "''Alcanivorax'' Which Prevails In Oil-contaminated Seawater Exhibits Broad Substrate Specificity For Alkane Degradation." Environmental Microbiology 5.9 (2003): 746-753.]<br />
<br />
[3] [http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html Lorenzo, Víctor De. "Blueprint of an Oil-eating Bacterium." Nature Biotechnology 24 (2006): 952-953.]<br />
<br />
[4] [http://www.ncbi.nlm.nih.gov/pubmed/16878126 Schneiker, S. et al. "Genome Sequence of the Ubiquitous Hydrocarbon-degrading Marine Bacterium A''lcanivorax Borkumensis''." Nature Biotechnology 24 (2006): 997-1004.]<br />
<br />
[5] [http://ijs.sgmjournals.org/cgi/content/abstract/48/2/339 Yakimov, Michail M., Peter N. Golyshin, Siegmund Lang, Edward R. B. Moore, Wolf-Rainer Abraham, Heinrich Lunsdorf, and Kenneth N. Timmis. "''Alcanivorax Borkumensis'' gen. nov., sp. nov., A New, Hydrocarbon-degrading And Surfactant-producing Marine Bacterium." International Journal of Systematic Bacteriology 48 (1998): 339-348.]<br />
<br />
==Author==<br />
Page authored by Andrew Buss, student of [http://www.kbs.msu.edu/faculty/lennon/ Prof. Jay Lennon] at Michigan State University.</div>Bussand1https://microbewiki.kenyon.edu/index.php?title=Alcanivorax&diff=29729Alcanivorax2008-04-06T18:56:52Z<p>Bussand1: /* Description and Significance */</p>
<hr />
<div>[[Image:AlcVorax PROCARYOTES01b-1.JPG|thumbnail|300px|Figure 1. ''Alcanivorax borkumensis''. Image from Helmholtz Centre for Infection Research[http://www.helmholtz-hzi.de/en/news_public_relation/press_releases/view/article/complete/oil_tanker_accidents_as_a_source_of_food/]]]<br />
<br />
==Classification==<br />
<br />
'''Bacteria'''; Phylum: '''Proteobacteria'''; Class: '''Gammaproteobacteria'''; Order: '''Oceanospirillales'''; Family: '''Alcanivoracaceae'''<br />
<br />
===Species===<br />
<br />
{|<br />
| height="10" bgcolor="#FFDF95" |<br />
'''NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Tree&id=2&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy]'''<br />
|}<br />
<br />
*''Alcanivorax balearicum''<br />
*''Alcanivorax borkumensis''<br />
*''Alcanivorax dieselolei''<br />
*''Alcanivorax indicus''<br />
*''Alcanivorax jadensis''<br />
*''Alcanivorax venustensis''<br />
<br />
==Description and Significance==<br />
[[Image:PWS tanker oil spill.jpg|thumbnail|200px|Figure 2. Supertanker Exxon Valdez grounded on Bligh Reef which released 11 million gallons of crude oil into the water. This oil-contaminated seawater is the preferred habitat for ''Alcanivorax''. Image from USGS[http://menlocampus.wr.usgs.gov/50years/accomplishments/oil.html]]]<br />
<br />
''Alcanivorax'', first described in 1998, is a Gram-negative, halophilic, aerobic, rod-shaped, oil-degrading marine bacterium that is found in low abundances in unpolluted environments in the upper layers of the ocean, but quickly becomes the predominant microbes in oil-contaminated open oceans and coastal waters when nitrogen and phosphorus are not limiting. When conditions in these moderately halophilic environments are right, ''Alcanivorax'' may make up 80-90% of the oil-degrading microbial community. It is described as a non-motile bactertium which is true for species such as ''Alcanivorax borkumensis'', but other species such as ''Alcanivorax venustensis'' were described to be motile by polar flagella (Fernandez-Martinez et al., 2003). <br />
<br />
As a result of their profound ability to degrade and live predominately on alkanes, as well as to become the dominant microbes in oil-contaminated areas, ''Alcanivorax'' plays a huge role in the biological cleanup of oil-contaminated environments. These oil-contaminated environments in the ocean are largely due to anthropogenic sources such as oil spills caused by tankers accidents, and cause serious ecological damage to coastal fauna and flora as well as other inhabitants of the ocean. Microbes such as ''Alcanivorax'' provide a major route for the breakdown of these pollutants, and demonstrate how marine bacteria keep the environment in check. Of all the ''Alcanivorax'' species and other oil-degrading microbes, ''Alcanivorax borkumensis'' is one of the most important worldwide due to the fact it produces a wide variety of very efficient oil-degrading enzymes. With this knowledge, ''A. borkumensis'' could provide a useful tool for bioremediation of oil spills.<br />
<br />
==Genome Structure==<br />
[[Image:Lorenzo.gif|thumbnail|200px|Figure 3. Mechanisms for oil degradation and survival encoded by the ''A. borkumensis SK2'' genome. Image from Victor de Lorenzo[http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html]]]<br />
<br />
The ''Alcanivorax borkumensis'' strain SK2 was the first hydrocarbonoclastic bacterium to be sequenced and was completed by Susanne Schneiker et al. It's genome consists of a single circular chromosome with 3,120,143 base pairs with an average G+C content of 54.7%. The genomic analysis of ''A. borkumensis SK2'' revealed several new insights into the bacterium's role for (i) n-alkane degradation (which includes metabolism, biosurfactant production and biofilm production), (ii) it's system for capturing or scavenging the small amounts of nitrogen, phosphorous, sulfur, and other elements in a nutrient-poor marine environment which allows for more efficient alkane degradation due to their main limitation of nutrient availability, (iii) as well as means for coping with stress factors such as high salt contents and high UV radiation since it thrives mostly in the upper layers in the ocean where UV light is encountered. It's genome encodes several systems for the catabolism of hydrocarbons which allow the bacertium to degrade all sorts of alkanes such as AlkB1 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C5-C12, and AlkB2 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C8 to C16. Both these systems are located close to the origin of replication of the chromosome. ''A. borkumensis'' is also able to degrade alkanes up to C32, branched aliphatic hydrocarbons, isoprenoid hydrocarbons such as phytane, as well as alkylarenes and alkylcycloalkanes. Thus, the genome encodes for a broad spectrum of systems for the catabolism of hydrocarbons, giving it a competitive advantage over other oil-degrading marine microbial communities. To deal with the damaging effects of UV light, ''A. borkumensis'' has a number of genes that reduce the damage. These include the full genes for DNA alkylation, recombinational and nucleotide excision repair, base excision repair, as well as the SOS response (Schneiker et al., 2006).<br />
<br />
==Cell Structure, Metabolism and Life Cycle==<br />
Interesting features of cell structure; how it gains energy; what important molecules it produces.<br />
<br />
<br />
==Ecology and Pathogenesis==<br />
Habitat; symbiosis; biogeochemical significance; contributions to environment.<br><br />
If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.<br><br><br />
<br />
==References==<br />
[1] [http://ijs.sgmjournals.org/cgi/content/abstract/53/1/331 Fernandez-Martinez, Javier, Maria J. Pujalte, Jesus Garcia-Martinez, Manuel Mata, Esperanza Garay, and Francisco Rodriguez-Valera. "Description of ''Alcanivorax Venustensis'' sp. nov. and Reclassification of ''Fundibacter Jadensis'' DSM 12178T (Bruns and Berthe-Corti 1999) As ''Alcanivorax Jadensis'' comb. nov., Members of the Emended Genus ''Alcanivorax''." International Journal of Systematic and Evolutionary Microbiology 53 (2003): 331-338.]<br />
<br />
[2] [http://www.blackwell-synergy.com/doi/pdf/10.1046/j.1468-2920.2003.00468.x Hara, Akihiro, Kazuaki Syutsubo, and Shigeaki Harayama. "''Alcanivorax'' Which Prevails In Oil-contaminated Seawater Exhibits Broad Substrate Specificity For Alkane Degradation." Environmental Microbiology 5.9 (2003): 746-753.]<br />
<br />
[3] [http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html Lorenzo, Víctor De. "Blueprint of an Oil-eating Bacterium." Nature Biotechnology 24 (2006): 952-953.]<br />
<br />
[4] [http://www.ncbi.nlm.nih.gov/pubmed/16878126 Schneiker, S. et al. "Genome Sequence of the Ubiquitous Hydrocarbon-degrading Marine Bacterium A''lcanivorax Borkumensis''." Nature Biotechnology 24 (2006): 997-1004.]<br />
<br />
[5] [http://ijs.sgmjournals.org/cgi/content/abstract/48/2/339 Yakimov, Michail M., Peter N. Golyshin, Siegmund Lang, Edward R. B. Moore, Wolf-Rainer Abraham, Heinrich Lunsdorf, and Kenneth N. Timmis. "''Alcanivorax Borkumensis'' gen. nov., sp. nov., A New, Hydrocarbon-degrading And Surfactant-producing Marine Bacterium." International Journal of Systematic Bacteriology 48 (1998): 339-348.]<br />
<br />
==Author==<br />
Page authored by Andrew Buss, student of [http://www.kbs.msu.edu/faculty/lennon/ Prof. Jay Lennon] at Michigan State University.</div>Bussand1https://microbewiki.kenyon.edu/index.php?title=Alcanivorax&diff=29728Alcanivorax2008-04-06T18:56:19Z<p>Bussand1: /* Description and Significance */</p>
<hr />
<div>[[Image:AlcVorax PROCARYOTES01b-1.JPG|thumbnail|300px|Figure 1. ''Alcanivorax borkumensis''. Image from Helmholtz Centre for Infection Research[http://www.helmholtz-hzi.de/en/news_public_relation/press_releases/view/article/complete/oil_tanker_accidents_as_a_source_of_food/]]]<br />
<br />
==Classification==<br />
<br />
'''Bacteria'''; Phylum: '''Proteobacteria'''; Class: '''Gammaproteobacteria'''; Order: '''Oceanospirillales'''; Family: '''Alcanivoracaceae'''<br />
<br />
===Species===<br />
<br />
{|<br />
| height="10" bgcolor="#FFDF95" |<br />
'''NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Tree&id=2&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy]'''<br />
|}<br />
<br />
*''Alcanivorax balearicum''<br />
*''Alcanivorax borkumensis''<br />
*''Alcanivorax dieselolei''<br />
*''Alcanivorax indicus''<br />
*''Alcanivorax jadensis''<br />
*''Alcanivorax venustensis''<br />
<br />
==Description and Significance==<br />
[[Image:PWS tanker oil spill.jpg|thumbnail|200px|Figure 2. Supertanker Exxon Valdez grounded on Bligh Reef which released 11 million gallons of crude oil into the water. This oil-contaminated seawater is the preferred habitat for ''Alcanivorax''. Image from USGS[http://menlocampus.wr.usgs.gov/50years/accomplishments/oil.html]]]<br />
<br />
''Alcanivorax'', first described in 1998, is a Gram-negative, halophilic, aerobic, rod-shaped, oil-degrading marine bacterium that is found in low abundances in unpolluted environments in the upper layers of the ocean, but quickly becomes the predominant microbes in oil-contaminated open oceans and coastal waters when nitrogen and phosphorus are not limiting. When conditions in these moderately halophilic environments are right, ''Alcanivorax'' may make up 80-90% of the oil-degrading microbial community. It is described as a non-motile bactertium which is true for species such as ''Alcanivorax borkumensis'', but other species such as ''Alcanivorax venustensis'' were described to be motile by polar flagella (Fernandez-Martinez et al., 2003). <br />
As a result of their profound ability to degrade and live predominately on alkanes, as well as to become the dominant microbes in oil-contaminated areas, ''Alcanivorax'' plays a huge role in the biological cleanup of oil-contaminated environments. These oil-contaminated environments in the ocean are largely due to anthropogenic sources such as oil spills caused by tankers accidents, and cause serious ecological damage to coastal fauna and flora as well as other inhabitants of the ocean. Microbes such as ''Alcanivorax'' provide a major route for the breakdown of these pollutants, and demonstrate how marine bacteria keep the environment in check. Of all the ''Alcanivorax'' species and other oil-degrading microbes, ''Alcanivorax borkumensis'' is one of the most important worldwide due to the fact it produces a wide variety of very efficient oil-degrading enzymes. With this knowledge, ''A. borkumensis'' could provide a useful tool for bioremediation of oil spills.<br />
<br />
==Genome Structure==<br />
[[Image:Lorenzo.gif|thumbnail|200px|Figure 3. Mechanisms for oil degradation and survival encoded by the ''A. borkumensis SK2'' genome. Image from Victor de Lorenzo[http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html]]]<br />
<br />
The ''Alcanivorax borkumensis'' strain SK2 was the first hydrocarbonoclastic bacterium to be sequenced and was completed by Susanne Schneiker et al. It's genome consists of a single circular chromosome with 3,120,143 base pairs with an average G+C content of 54.7%. The genomic analysis of ''A. borkumensis SK2'' revealed several new insights into the bacterium's role for (i) n-alkane degradation (which includes metabolism, biosurfactant production and biofilm production), (ii) it's system for capturing or scavenging the small amounts of nitrogen, phosphorous, sulfur, and other elements in a nutrient-poor marine environment which allows for more efficient alkane degradation due to their main limitation of nutrient availability, (iii) as well as means for coping with stress factors such as high salt contents and high UV radiation since it thrives mostly in the upper layers in the ocean where UV light is encountered. It's genome encodes several systems for the catabolism of hydrocarbons which allow the bacertium to degrade all sorts of alkanes such as AlkB1 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C5-C12, and AlkB2 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C8 to C16. Both these systems are located close to the origin of replication of the chromosome. ''A. borkumensis'' is also able to degrade alkanes up to C32, branched aliphatic hydrocarbons, isoprenoid hydrocarbons such as phytane, as well as alkylarenes and alkylcycloalkanes. Thus, the genome encodes for a broad spectrum of systems for the catabolism of hydrocarbons, giving it a competitive advantage over other oil-degrading marine microbial communities. To deal with the damaging effects of UV light, ''A. borkumensis'' has a number of genes that reduce the damage. These include the full genes for DNA alkylation, recombinational and nucleotide excision repair, base excision repair, as well as the SOS response (Schneiker et al., 2006).<br />
<br />
==Cell Structure, Metabolism and Life Cycle==<br />
Interesting features of cell structure; how it gains energy; what important molecules it produces.<br />
<br />
<br />
==Ecology and Pathogenesis==<br />
Habitat; symbiosis; biogeochemical significance; contributions to environment.<br><br />
If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.<br><br><br />
<br />
==References==<br />
[1] [http://ijs.sgmjournals.org/cgi/content/abstract/53/1/331 Fernandez-Martinez, Javier, Maria J. Pujalte, Jesus Garcia-Martinez, Manuel Mata, Esperanza Garay, and Francisco Rodriguez-Valera. "Description of ''Alcanivorax Venustensis'' sp. nov. and Reclassification of ''Fundibacter Jadensis'' DSM 12178T (Bruns and Berthe-Corti 1999) As ''Alcanivorax Jadensis'' comb. nov., Members of the Emended Genus ''Alcanivorax''." International Journal of Systematic and Evolutionary Microbiology 53 (2003): 331-338.]<br />
<br />
[2] [http://www.blackwell-synergy.com/doi/pdf/10.1046/j.1468-2920.2003.00468.x Hara, Akihiro, Kazuaki Syutsubo, and Shigeaki Harayama. "''Alcanivorax'' Which Prevails In Oil-contaminated Seawater Exhibits Broad Substrate Specificity For Alkane Degradation." Environmental Microbiology 5.9 (2003): 746-753.]<br />
<br />
[3] [http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html Lorenzo, Víctor De. "Blueprint of an Oil-eating Bacterium." Nature Biotechnology 24 (2006): 952-953.]<br />
<br />
[4] [http://www.ncbi.nlm.nih.gov/pubmed/16878126 Schneiker, S. et al. "Genome Sequence of the Ubiquitous Hydrocarbon-degrading Marine Bacterium A''lcanivorax Borkumensis''." Nature Biotechnology 24 (2006): 997-1004.]<br />
<br />
[5] [http://ijs.sgmjournals.org/cgi/content/abstract/48/2/339 Yakimov, Michail M., Peter N. Golyshin, Siegmund Lang, Edward R. B. Moore, Wolf-Rainer Abraham, Heinrich Lunsdorf, and Kenneth N. Timmis. "''Alcanivorax Borkumensis'' gen. nov., sp. nov., A New, Hydrocarbon-degrading And Surfactant-producing Marine Bacterium." International Journal of Systematic Bacteriology 48 (1998): 339-348.]<br />
<br />
==Author==<br />
Page authored by Andrew Buss, student of [http://www.kbs.msu.edu/faculty/lennon/ Prof. Jay Lennon] at Michigan State University.</div>Bussand1https://microbewiki.kenyon.edu/index.php?title=Alcanivorax&diff=29727Alcanivorax2008-04-06T18:53:20Z<p>Bussand1: /* Genome Structure */</p>
<hr />
<div>[[Image:AlcVorax PROCARYOTES01b-1.JPG|thumbnail|300px|Figure 1. ''Alcanivorax borkumensis''. Image from Helmholtz Centre for Infection Research[http://www.helmholtz-hzi.de/en/news_public_relation/press_releases/view/article/complete/oil_tanker_accidents_as_a_source_of_food/]]]<br />
<br />
==Classification==<br />
<br />
'''Bacteria'''; Phylum: '''Proteobacteria'''; Class: '''Gammaproteobacteria'''; Order: '''Oceanospirillales'''; Family: '''Alcanivoracaceae'''<br />
<br />
===Species===<br />
<br />
{|<br />
| height="10" bgcolor="#FFDF95" |<br />
'''NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Tree&id=2&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy]'''<br />
|}<br />
<br />
*''Alcanivorax balearicum''<br />
*''Alcanivorax borkumensis''<br />
*''Alcanivorax dieselolei''<br />
*''Alcanivorax indicus''<br />
*''Alcanivorax jadensis''<br />
*''Alcanivorax venustensis''<br />
<br />
==Description and Significance==<br />
[[Image:PWS tanker oil spill.jpg|thumbnail|200px|Figure 2. Supertanker Exxon Valdez grounded on Bligh Reef which released 11 million gallons of crude oil into the water. This oil-contaminated seawater is the preferred habitat for ''Alcanivorax''. Image from USGS[http://menlocampus.wr.usgs.gov/50years/accomplishments/oil.html]]]<br />
<br />
''Alcanivorax'', first described in 1998, is a Gram-negative, aerobic, rod-shaped, oil-degrading marine bacterium that is found in low abundances in unpolluted environments in the upper layers of the ocean, but quickly becomes the predominant microbes in oil-contaminated open oceans and coastal waters when nitrogen and phosphorus are not limiting. When conditions in these moderately halophilic environments are right, ''Alcanivorax'' may make up 80-90% of the oil-degrading microbial community. It is described as a non-motile bactertium which is true for species such as ''Alcanivorax borkumensis'', but other species such as ''Alcanivorax venustensis'' were described to be motile by polar flagella (Fernandez-Martinez et al., 2003). As a result of their profound ability to degrade and live predominately on alkanes, as well as to become the dominant microbes in oil-contaminated areas, ''Alcanivorax'' plays a huge role in the biological cleanup of oil-contaminated environments. These oil-contaminated environments in the ocean are largely due to anthropogenic sources such as oil spills caused by tankers accidents, and cause serious ecological damage to coastal fauna and flora as well as other inhabitants of the ocean. Microbes such as ''Alcanivorax'' provide a major route for the breakdown of these pollutants, and demonstrate how marine bacteria keep the environment in check. Of all the ''Alcanivorax'' species and other oil-degrading microbes, ''Alcanivorax borkumensis'' is one of the most important worldwide due to the fact it produces a wide variety of very efficient oil-degrading enzymes. With this knowledge, ''A. borkumensis'' could provide a useful tool for bioremediation of oil spills.<br />
<br />
==Genome Structure==<br />
[[Image:Lorenzo.gif|thumbnail|200px|Figure 3. Mechanisms for oil degradation and survival encoded by the ''A. borkumensis SK2'' genome. Image from Victor de Lorenzo[http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html]]]<br />
<br />
The ''Alcanivorax borkumensis'' strain SK2 was the first hydrocarbonoclastic bacterium to be sequenced and was completed by Susanne Schneiker et al. It's genome consists of a single circular chromosome with 3,120,143 base pairs with an average G+C content of 54.7%. The genomic analysis of ''A. borkumensis SK2'' revealed several new insights into the bacterium's role for (i) n-alkane degradation (which includes metabolism, biosurfactant production and biofilm production), (ii) it's system for capturing or scavenging the small amounts of nitrogen, phosphorous, sulfur, and other elements in a nutrient-poor marine environment which allows for more efficient alkane degradation due to their main limitation of nutrient availability, (iii) as well as means for coping with stress factors such as high salt contents and high UV radiation since it thrives mostly in the upper layers in the ocean where UV light is encountered. It's genome encodes several systems for the catabolism of hydrocarbons which allow the bacertium to degrade all sorts of alkanes such as AlkB1 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C5-C12, and AlkB2 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C8 to C16. Both these systems are located close to the origin of replication of the chromosome. ''A. borkumensis'' is also able to degrade alkanes up to C32, branched aliphatic hydrocarbons, isoprenoid hydrocarbons such as phytane, as well as alkylarenes and alkylcycloalkanes. Thus, the genome encodes for a broad spectrum of systems for the catabolism of hydrocarbons, giving it a competitive advantage over other oil-degrading marine microbial communities. To deal with the damaging effects of UV light, ''A. borkumensis'' has a number of genes that reduce the damage. These include the full genes for DNA alkylation, recombinational and nucleotide excision repair, base excision repair, as well as the SOS response (Schneiker et al., 2006).<br />
<br />
==Cell Structure, Metabolism and Life Cycle==<br />
Interesting features of cell structure; how it gains energy; what important molecules it produces.<br />
<br />
<br />
==Ecology and Pathogenesis==<br />
Habitat; symbiosis; biogeochemical significance; contributions to environment.<br><br />
If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.<br><br><br />
<br />
==References==<br />
[1] [http://ijs.sgmjournals.org/cgi/content/abstract/53/1/331 Fernandez-Martinez, Javier, Maria J. Pujalte, Jesus Garcia-Martinez, Manuel Mata, Esperanza Garay, and Francisco Rodriguez-Valera. "Description of ''Alcanivorax Venustensis'' sp. nov. and Reclassification of ''Fundibacter Jadensis'' DSM 12178T (Bruns and Berthe-Corti 1999) As ''Alcanivorax Jadensis'' comb. nov., Members of the Emended Genus ''Alcanivorax''." International Journal of Systematic and Evolutionary Microbiology 53 (2003): 331-338.]<br />
<br />
[2] [http://www.blackwell-synergy.com/doi/pdf/10.1046/j.1468-2920.2003.00468.x Hara, Akihiro, Kazuaki Syutsubo, and Shigeaki Harayama. "''Alcanivorax'' Which Prevails In Oil-contaminated Seawater Exhibits Broad Substrate Specificity For Alkane Degradation." Environmental Microbiology 5.9 (2003): 746-753.]<br />
<br />
[3] [http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html Lorenzo, Víctor De. "Blueprint of an Oil-eating Bacterium." Nature Biotechnology 24 (2006): 952-953.]<br />
<br />
[4] [http://www.ncbi.nlm.nih.gov/pubmed/16878126 Schneiker, S. et al. "Genome Sequence of the Ubiquitous Hydrocarbon-degrading Marine Bacterium A''lcanivorax Borkumensis''." Nature Biotechnology 24 (2006): 997-1004.]<br />
<br />
[5] [http://ijs.sgmjournals.org/cgi/content/abstract/48/2/339 Yakimov, Michail M., Peter N. Golyshin, Siegmund Lang, Edward R. B. Moore, Wolf-Rainer Abraham, Heinrich Lunsdorf, and Kenneth N. Timmis. "''Alcanivorax Borkumensis'' gen. nov., sp. nov., A New, Hydrocarbon-degrading And Surfactant-producing Marine Bacterium." International Journal of Systematic Bacteriology 48 (1998): 339-348.]<br />
<br />
==Author==<br />
Page authored by Andrew Buss, student of [http://www.kbs.msu.edu/faculty/lennon/ Prof. Jay Lennon] at Michigan State University.</div>Bussand1https://microbewiki.kenyon.edu/index.php?title=Alcanivorax&diff=29726Alcanivorax2008-04-06T18:53:11Z<p>Bussand1: /* Description and Significance */</p>
<hr />
<div>[[Image:AlcVorax PROCARYOTES01b-1.JPG|thumbnail|300px|Figure 1. ''Alcanivorax borkumensis''. Image from Helmholtz Centre for Infection Research[http://www.helmholtz-hzi.de/en/news_public_relation/press_releases/view/article/complete/oil_tanker_accidents_as_a_source_of_food/]]]<br />
<br />
==Classification==<br />
<br />
'''Bacteria'''; Phylum: '''Proteobacteria'''; Class: '''Gammaproteobacteria'''; Order: '''Oceanospirillales'''; Family: '''Alcanivoracaceae'''<br />
<br />
===Species===<br />
<br />
{|<br />
| height="10" bgcolor="#FFDF95" |<br />
'''NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Tree&id=2&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy]'''<br />
|}<br />
<br />
*''Alcanivorax balearicum''<br />
*''Alcanivorax borkumensis''<br />
*''Alcanivorax dieselolei''<br />
*''Alcanivorax indicus''<br />
*''Alcanivorax jadensis''<br />
*''Alcanivorax venustensis''<br />
<br />
==Description and Significance==<br />
[[Image:PWS tanker oil spill.jpg|thumbnail|200px|Figure 2. Supertanker Exxon Valdez grounded on Bligh Reef which released 11 million gallons of crude oil into the water. This oil-contaminated seawater is the preferred habitat for ''Alcanivorax''. Image from USGS[http://menlocampus.wr.usgs.gov/50years/accomplishments/oil.html]]]<br />
<br />
''Alcanivorax'', first described in 1998, is a Gram-negative, aerobic, rod-shaped, oil-degrading marine bacterium that is found in low abundances in unpolluted environments in the upper layers of the ocean, but quickly becomes the predominant microbes in oil-contaminated open oceans and coastal waters when nitrogen and phosphorus are not limiting. When conditions in these moderately halophilic environments are right, ''Alcanivorax'' may make up 80-90% of the oil-degrading microbial community. It is described as a non-motile bactertium which is true for species such as ''Alcanivorax borkumensis'', but other species such as ''Alcanivorax venustensis'' were described to be motile by polar flagella (Fernandez-Martinez et al., 2003). As a result of their profound ability to degrade and live predominately on alkanes, as well as to become the dominant microbes in oil-contaminated areas, ''Alcanivorax'' plays a huge role in the biological cleanup of oil-contaminated environments. These oil-contaminated environments in the ocean are largely due to anthropogenic sources such as oil spills caused by tankers accidents, and cause serious ecological damage to coastal fauna and flora as well as other inhabitants of the ocean. Microbes such as ''Alcanivorax'' provide a major route for the breakdown of these pollutants, and demonstrate how marine bacteria keep the environment in check. Of all the ''Alcanivorax'' species and other oil-degrading microbes, ''Alcanivorax borkumensis'' is one of the most important worldwide due to the fact it produces a wide variety of very efficient oil-degrading enzymes. With this knowledge, ''A. borkumensis'' could provide a useful tool for bioremediation of oil spills.<br />
<br />
==Genome Structure==<br />
[[Image:Lorenzo.gif|thumbnail|200px|Figure 3. Mechanisms for oil degradation and survival encoded by the ''A. borkumensis SK2'' genome. Image from Victor de Lorenzo[http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html]]]<br />
<br />
The ''Alcanivorax borkumensis'' strain SK2 was the first hydrocarbonoclastic bacterium to be sequenced and was completed by Susanne Schneiker et al. It's genome consists of a single circular chromosome with 3,120,143 base pairs with an average G+C content of 54.7%. The genomic analysis of ''A. borkumensis SK2'' revealed several new insights into the bacterium's role for (i) n-alkane degradation (which includes metabolism, biosurfactant production and biofilm production), (ii) it's system for capturing or scavenging the small amounts of nitrogen, phosphorous, sulfur, and other elements in a nutrient-poor marine environment which allows for more efficient alkane degradation due to their main limitation of nutrient availability, (iii) as well as means for coping with stress factors such as high salt contents and high UV radiation since it thrives mostly in the upper layers in the ocean where UV light is encountered. It's genome encodes several systems for the catabolism of hydrocarbons which allow the bacertium to degrade all sorts of alkanes such as AlkB1 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C5-C12, and AlkB2 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C8 to C16. Both these systems are located close to the origin of replication of the chromosome. ''A. borkumensis'' is also able to degrade alkanes up to C32, branched aliphatic hydrocarbons, isoprenoid hydrocarbons such as phytane, as well as alkylarenes and alkylcycloalkanes. Thus, the genome encodes for a broad spectrum of systems for the catabolism of hydrocarbons, giving it a competitive advantage over other oil-degrading marine microbial communities. To deal with the damaging effects of UV light, ''A. borkumensis'' has a number of genes that reduce the damage. These include the full genes for DNA alkylation, recombinational and nucleotide excision repair, base excision repair, as well as the SOS response (Schneiker et al. 2006).<br />
<br />
==Cell Structure, Metabolism and Life Cycle==<br />
Interesting features of cell structure; how it gains energy; what important molecules it produces.<br />
<br />
<br />
==Ecology and Pathogenesis==<br />
Habitat; symbiosis; biogeochemical significance; contributions to environment.<br><br />
If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.<br><br><br />
<br />
==References==<br />
[1] [http://ijs.sgmjournals.org/cgi/content/abstract/53/1/331 Fernandez-Martinez, Javier, Maria J. Pujalte, Jesus Garcia-Martinez, Manuel Mata, Esperanza Garay, and Francisco Rodriguez-Valera. "Description of ''Alcanivorax Venustensis'' sp. nov. and Reclassification of ''Fundibacter Jadensis'' DSM 12178T (Bruns and Berthe-Corti 1999) As ''Alcanivorax Jadensis'' comb. nov., Members of the Emended Genus ''Alcanivorax''." International Journal of Systematic and Evolutionary Microbiology 53 (2003): 331-338.]<br />
<br />
[2] [http://www.blackwell-synergy.com/doi/pdf/10.1046/j.1468-2920.2003.00468.x Hara, Akihiro, Kazuaki Syutsubo, and Shigeaki Harayama. "''Alcanivorax'' Which Prevails In Oil-contaminated Seawater Exhibits Broad Substrate Specificity For Alkane Degradation." Environmental Microbiology 5.9 (2003): 746-753.]<br />
<br />
[3] [http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html Lorenzo, Víctor De. "Blueprint of an Oil-eating Bacterium." Nature Biotechnology 24 (2006): 952-953.]<br />
<br />
[4] [http://www.ncbi.nlm.nih.gov/pubmed/16878126 Schneiker, S. et al. "Genome Sequence of the Ubiquitous Hydrocarbon-degrading Marine Bacterium A''lcanivorax Borkumensis''." Nature Biotechnology 24 (2006): 997-1004.]<br />
<br />
[5] [http://ijs.sgmjournals.org/cgi/content/abstract/48/2/339 Yakimov, Michail M., Peter N. Golyshin, Siegmund Lang, Edward R. B. Moore, Wolf-Rainer Abraham, Heinrich Lunsdorf, and Kenneth N. Timmis. "''Alcanivorax Borkumensis'' gen. nov., sp. nov., A New, Hydrocarbon-degrading And Surfactant-producing Marine Bacterium." International Journal of Systematic Bacteriology 48 (1998): 339-348.]<br />
<br />
==Author==<br />
Page authored by Andrew Buss, student of [http://www.kbs.msu.edu/faculty/lennon/ Prof. Jay Lennon] at Michigan State University.</div>Bussand1https://microbewiki.kenyon.edu/index.php?title=Alcanivorax&diff=29725Alcanivorax2008-04-06T18:52:36Z<p>Bussand1: /* Description and Significance */</p>
<hr />
<div>[[Image:AlcVorax PROCARYOTES01b-1.JPG|thumbnail|300px|Figure 1. ''Alcanivorax borkumensis''. Image from Helmholtz Centre for Infection Research[http://www.helmholtz-hzi.de/en/news_public_relation/press_releases/view/article/complete/oil_tanker_accidents_as_a_source_of_food/]]]<br />
<br />
==Classification==<br />
<br />
'''Bacteria'''; Phylum: '''Proteobacteria'''; Class: '''Gammaproteobacteria'''; Order: '''Oceanospirillales'''; Family: '''Alcanivoracaceae'''<br />
<br />
===Species===<br />
<br />
{|<br />
| height="10" bgcolor="#FFDF95" |<br />
'''NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Tree&id=2&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy]'''<br />
|}<br />
<br />
*''Alcanivorax balearicum''<br />
*''Alcanivorax borkumensis''<br />
*''Alcanivorax dieselolei''<br />
*''Alcanivorax indicus''<br />
*''Alcanivorax jadensis''<br />
*''Alcanivorax venustensis''<br />
<br />
==Description and Significance==<br />
[[Image:PWS tanker oil spill.jpg|thumbnail|200px|Figure 2. Supertanker Exxon Valdez grounded on Bligh Reef which released 11 million gallons of crude oil into the water. This oil-contaminated seawater is the preferred habitat for ''Alcanivorax''. Image from USGS[http://menlocampus.wr.usgs.gov/50years/accomplishments/oil.html]]]<br />
<br />
''Alcanivorax'', first described in 1998, is a Gram-negative, aerobic, rod-shaped, oil-degrading marine bacterium that is found in low abundances in unpolluted environments in the upper layers of the ocean, but quickly becomes the predominant microbes in oil-contaminated open oceans and coastal waters when nitrogen and phosphorus are not limiting. When conditions in these moderately halophilic environments are right, ''Alcanivorax'' may make up 80-90% of the oil-degrading microbial community. It is described as a non-motile bactertium which is true for species such as ''Alcanivorax borkumensis'', but other species such as ''Alcanivorax venustensis'' were described to be motile by polar flagella (Fernandez-Martinez, 2003). As a result of their profound ability to degrade and live predominately on alkanes, as well as to become the dominant microbes in oil-contaminated areas, ''Alcanivorax'' plays a huge role in the biological cleanup of oil-contaminated environments. These oil-contaminated environments in the ocean are largely due to anthropogenic sources such as oil spills caused by tankers accidents, and cause serious ecological damage to coastal fauna and flora as well as other inhabitants of the ocean. Microbes such as ''Alcanivorax'' provide a major route for the breakdown of these pollutants, and demonstrate how marine bacteria keep the environment in check. Of all the ''Alcanivorax'' species and other oil-degrading microbes, ''Alcanivorax borkumensis'' is one of the most important worldwide due to the fact it produces a wide variety of very efficient oil-degrading enzymes. With this knowledge, ''A. borkumensis'' could provide a useful tool for bioremediation of oil spills.<br />
<br />
==Genome Structure==<br />
[[Image:Lorenzo.gif|thumbnail|200px|Figure 3. Mechanisms for oil degradation and survival encoded by the ''A. borkumensis SK2'' genome. Image from Victor de Lorenzo[http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html]]]<br />
<br />
The ''Alcanivorax borkumensis'' strain SK2 was the first hydrocarbonoclastic bacterium to be sequenced and was completed by Susanne Schneiker et al. It's genome consists of a single circular chromosome with 3,120,143 base pairs with an average G+C content of 54.7%. The genomic analysis of ''A. borkumensis SK2'' revealed several new insights into the bacterium's role for (i) n-alkane degradation (which includes metabolism, biosurfactant production and biofilm production), (ii) it's system for capturing or scavenging the small amounts of nitrogen, phosphorous, sulfur, and other elements in a nutrient-poor marine environment which allows for more efficient alkane degradation due to their main limitation of nutrient availability, (iii) as well as means for coping with stress factors such as high salt contents and high UV radiation since it thrives mostly in the upper layers in the ocean where UV light is encountered. It's genome encodes several systems for the catabolism of hydrocarbons which allow the bacertium to degrade all sorts of alkanes such as AlkB1 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C5-C12, and AlkB2 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C8 to C16. Both these systems are located close to the origin of replication of the chromosome. ''A. borkumensis'' is also able to degrade alkanes up to C32, branched aliphatic hydrocarbons, isoprenoid hydrocarbons such as phytane, as well as alkylarenes and alkylcycloalkanes. Thus, the genome encodes for a broad spectrum of systems for the catabolism of hydrocarbons, giving it a competitive advantage over other oil-degrading marine microbial communities. To deal with the damaging effects of UV light, ''A. borkumensis'' has a number of genes that reduce the damage. These include the full genes for DNA alkylation, recombinational and nucleotide excision repair, base excision repair, as well as the SOS response (Schneiker et al. 2006).<br />
<br />
==Cell Structure, Metabolism and Life Cycle==<br />
Interesting features of cell structure; how it gains energy; what important molecules it produces.<br />
<br />
<br />
==Ecology and Pathogenesis==<br />
Habitat; symbiosis; biogeochemical significance; contributions to environment.<br><br />
If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.<br><br><br />
<br />
==References==<br />
[1] [http://ijs.sgmjournals.org/cgi/content/abstract/53/1/331 Fernandez-Martinez, Javier, Maria J. Pujalte, Jesus Garcia-Martinez, Manuel Mata, Esperanza Garay, and Francisco Rodriguez-Valera. "Description of ''Alcanivorax Venustensis'' sp. nov. and Reclassification of ''Fundibacter Jadensis'' DSM 12178T (Bruns and Berthe-Corti 1999) As ''Alcanivorax Jadensis'' comb. nov., Members of the Emended Genus ''Alcanivorax''." International Journal of Systematic and Evolutionary Microbiology 53 (2003): 331-338.]<br />
<br />
[2] [http://www.blackwell-synergy.com/doi/pdf/10.1046/j.1468-2920.2003.00468.x Hara, Akihiro, Kazuaki Syutsubo, and Shigeaki Harayama. "''Alcanivorax'' Which Prevails In Oil-contaminated Seawater Exhibits Broad Substrate Specificity For Alkane Degradation." Environmental Microbiology 5.9 (2003): 746-753.]<br />
<br />
[3] [http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html Lorenzo, Víctor De. "Blueprint of an Oil-eating Bacterium." Nature Biotechnology 24 (2006): 952-953.]<br />
<br />
[4] [http://www.ncbi.nlm.nih.gov/pubmed/16878126 Schneiker, S. et al. "Genome Sequence of the Ubiquitous Hydrocarbon-degrading Marine Bacterium A''lcanivorax Borkumensis''." Nature Biotechnology 24 (2006): 997-1004.]<br />
<br />
[5] [http://ijs.sgmjournals.org/cgi/content/abstract/48/2/339 Yakimov, Michail M., Peter N. Golyshin, Siegmund Lang, Edward R. B. Moore, Wolf-Rainer Abraham, Heinrich Lunsdorf, and Kenneth N. Timmis. "''Alcanivorax Borkumensis'' gen. nov., sp. nov., A New, Hydrocarbon-degrading And Surfactant-producing Marine Bacterium." International Journal of Systematic Bacteriology 48 (1998): 339-348.]<br />
<br />
==Author==<br />
Page authored by Andrew Buss, student of [http://www.kbs.msu.edu/faculty/lennon/ Prof. Jay Lennon] at Michigan State University.</div>Bussand1https://microbewiki.kenyon.edu/index.php?title=Alcanivorax&diff=29718Alcanivorax2008-04-06T01:37:26Z<p>Bussand1: /* Genome Structure */</p>
<hr />
<div>[[Image:AlcVorax PROCARYOTES01b-1.JPG|thumbnail|300px|Figure 1. ''Alcanivorax borkumensis''. Image from Helmholtz Centre for Infection Research[http://www.helmholtz-hzi.de/en/news_public_relation/press_releases/view/article/complete/oil_tanker_accidents_as_a_source_of_food/]]]<br />
<br />
==Classification==<br />
<br />
'''Bacteria'''; Phylum: '''Proteobacteria'''; Class: '''Gammaproteobacteria'''; Order: '''Oceanospirillales'''; Family: '''Alcanivoracaceae'''<br />
<br />
===Species===<br />
<br />
{|<br />
| height="10" bgcolor="#FFDF95" |<br />
'''NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Tree&id=2&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy]'''<br />
|}<br />
<br />
*''Alcanivorax balearicum''<br />
*''Alcanivorax borkumensis''<br />
*''Alcanivorax dieselolei''<br />
*''Alcanivorax indicus''<br />
*''Alcanivorax jadensis''<br />
*''Alcanivorax venustensis''<br />
<br />
==Description and Significance==<br />
[[Image:PWS tanker oil spill.jpg|thumbnail|200px|Figure 2. Supertanker Exxon Valdez grounded on Bligh Reef which released 11 million gallons of crude oil into the water. This oil-contaminated seawater is the preferred habitat for ''Alcanivorax''. Image from USGS[http://menlocampus.wr.usgs.gov/50years/accomplishments/oil.html]]]<br />
<br />
''Alcanivorax'', first described in 1998, is a Gram-negative, aerobic, rod-shaped, oil-degrading marine bacterium that is found in low abundances in unpolluted environments in the upper layers of the ocean, but quickly becomes the predominant microbes in oil-contaminated open oceans and coastal waters when nitrogen and phosphorus are not limiting. When conditions in these moderately halophilic environments are right, ''Alcanivorax'' may make up 80-90% of the oil-degrading microbial community. As a result of their profound ability to degrade and live predominately on alkanes, as well as to become the dominant microbes in oil-contaminated areas, ''Alcanivorax'' plays a huge role in the biological cleanup of oil-contaminated environments. These oil-contaminated environments in the ocean are largely due to anthropogenic sources such as oil spills caused by tankers accidents, and cause serious ecological damage to coastal fauna and flora as well as other inhabitants of the ocean. Microbes such as ''Alcanivorax'' provide a major route for the breakdown of these pollutants, and demonstrate how marine bacteria keep the environment in check. Of all the ''Alcanivorax'' species and other oil-degrading microbes, ''Alcanivorax borkumensis'' is one of the most important worldwide due to the fact it produces a wide variety of very efficient oil-degrading enzymes. With this knowledge, ''Alcanivorax borkumensis'' could provide a useful tool for bioremediation of oil spills.<br />
<br />
==Genome Structure==<br />
[[Image:Lorenzo.gif|thumbnail|200px|Figure 3. Mechanisms for oil degradation and survival encoded by the ''A. borkumensis SK2'' genome. Image from Victor de Lorenzo[http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html]]]<br />
<br />
The ''Alcanivorax borkumensis'' strain SK2 was the first hydrocarbonoclastic bacterium to be sequenced and was completed by Susanne Schneiker et al. It's genome consists of a single circular chromosome with 3,120,143 base pairs with an average G+C content of 54.7%. The genomic analysis of ''A. borkumensis SK2'' revealed several new insights into the bacterium's role for (i) n-alkane degradation (which includes metabolism, biosurfactant production and biofilm production), (ii) it's system for capturing or scavenging the small amounts of nitrogen, phosphorous, sulfur, and other elements in a nutrient-poor marine environment which allows for more efficient alkane degradation due to their main limitation of nutrient availability, (iii) as well as means for coping with stress factors such as high salt contents and high UV radiation since it thrives mostly in the upper layers in the ocean where UV light is encountered. It's genome encodes several systems for the catabolism of hydrocarbons which allow the bacertium to degrade all sorts of alkanes such as AlkB1 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C5-C12, and AlkB2 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C8 to C16. Both these systems are located close to the origin of replication of the chromosome. ''A. borkumensis'' is also able to degrade alkanes up to C32, branched aliphatic hydrocarbons, isoprenoid hydrocarbons such as phytane, as well as alkylarenes and alkylcycloalkanes. Thus, the genome encodes for a broad spectrum of systems for the catabolism of hydrocarbons, giving it a competitive advantage over other oil-degrading marine microbial communities. To deal with the damaging effects of UV light, ''A. borkumensis'' has a number of genes that reduce the damage. These include the full genes for DNA alkylation, recombinational and nucleotide excision repair, base excision repair, as well as the SOS response (Schneiker et al. 2006).<br />
<br />
==Cell Structure, Metabolism and Life Cycle==<br />
Interesting features of cell structure; how it gains energy; what important molecules it produces.<br />
<br />
<br />
==Ecology and Pathogenesis==<br />
Habitat; symbiosis; biogeochemical significance; contributions to environment.<br><br />
If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.<br><br><br />
<br />
==References==<br />
[1] [http://ijs.sgmjournals.org/cgi/content/abstract/53/1/331 Fernandez-Martinez, Javier, Maria J. Pujalte, Jesus Garcia-Martinez, Manuel Mata, Esperanza Garay, and Francisco Rodriguez-Valera. "Description of ''Alcanivorax Venustensis'' sp. nov. and Reclassification of ''Fundibacter Jadensis'' DSM 12178T (Bruns and Berthe-Corti 1999) As ''Alcanivorax Jadensis'' comb. nov., Members of the Emended Genus ''Alcanivorax''." International Journal of Systematic and Evolutionary Microbiology 53 (2003): 331-338.]<br />
<br />
[2] [http://www.blackwell-synergy.com/doi/pdf/10.1046/j.1468-2920.2003.00468.x Hara, Akihiro, Kazuaki Syutsubo, and Shigeaki Harayama. "''Alcanivorax'' Which Prevails In Oil-contaminated Seawater Exhibits Broad Substrate Specificity For Alkane Degradation." Environmental Microbiology 5.9 (2003): 746-753.]<br />
<br />
[3] [http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html Lorenzo, Víctor De. "Blueprint of an Oil-eating Bacterium." Nature Biotechnology 24 (2006): 952-953.]<br />
<br />
[4] [http://www.ncbi.nlm.nih.gov/pubmed/16878126 Schneiker, S. et al. "Genome Sequence of the Ubiquitous Hydrocarbon-degrading Marine Bacterium A''lcanivorax Borkumensis''." Nature Biotechnology 24 (2006): 997-1004.]<br />
<br />
[5] [http://ijs.sgmjournals.org/cgi/content/abstract/48/2/339 Yakimov, Michail M., Peter N. Golyshin, Siegmund Lang, Edward R. B. Moore, Wolf-Rainer Abraham, Heinrich Lunsdorf, and Kenneth N. Timmis. "''Alcanivorax Borkumensis'' gen. nov., sp. nov., A New, Hydrocarbon-degrading And Surfactant-producing Marine Bacterium." International Journal of Systematic Bacteriology 48 (1998): 339-348.]<br />
<br />
==Author==<br />
Page authored by Andrew Buss, student of [http://www.kbs.msu.edu/faculty/lennon/ Prof. Jay Lennon] at Michigan State University.</div>Bussand1https://microbewiki.kenyon.edu/index.php?title=Alcanivorax&diff=29717Alcanivorax2008-04-05T23:07:10Z<p>Bussand1: /* Classification */</p>
<hr />
<div>[[Image:AlcVorax PROCARYOTES01b-1.JPG|thumbnail|300px|Figure 1. ''Alcanivorax borkumensis''. Image from Helmholtz Centre for Infection Research[http://www.helmholtz-hzi.de/en/news_public_relation/press_releases/view/article/complete/oil_tanker_accidents_as_a_source_of_food/]]]<br />
<br />
==Classification==<br />
<br />
'''Bacteria'''; Phylum: '''Proteobacteria'''; Class: '''Gammaproteobacteria'''; Order: '''Oceanospirillales'''; Family: '''Alcanivoracaceae'''<br />
<br />
===Species===<br />
<br />
{|<br />
| height="10" bgcolor="#FFDF95" |<br />
'''NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Tree&id=2&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy]'''<br />
|}<br />
<br />
*''Alcanivorax balearicum''<br />
*''Alcanivorax borkumensis''<br />
*''Alcanivorax dieselolei''<br />
*''Alcanivorax indicus''<br />
*''Alcanivorax jadensis''<br />
*''Alcanivorax venustensis''<br />
<br />
==Description and Significance==<br />
[[Image:PWS tanker oil spill.jpg|thumbnail|200px|Figure 2. Supertanker Exxon Valdez grounded on Bligh Reef which released 11 million gallons of crude oil into the water. This oil-contaminated seawater is the preferred habitat for ''Alcanivorax''. Image from USGS[http://menlocampus.wr.usgs.gov/50years/accomplishments/oil.html]]]<br />
<br />
''Alcanivorax'', first described in 1998, is a Gram-negative, aerobic, rod-shaped, oil-degrading marine bacterium that is found in low abundances in unpolluted environments in the upper layers of the ocean, but quickly becomes the predominant microbes in oil-contaminated open oceans and coastal waters when nitrogen and phosphorus are not limiting. When conditions in these moderately halophilic environments are right, ''Alcanivorax'' may make up 80-90% of the oil-degrading microbial community. As a result of their profound ability to degrade and live predominately on alkanes, as well as to become the dominant microbes in oil-contaminated areas, ''Alcanivorax'' plays a huge role in the biological cleanup of oil-contaminated environments. These oil-contaminated environments in the ocean are largely due to anthropogenic sources such as oil spills caused by tankers accidents, and cause serious ecological damage to coastal fauna and flora as well as other inhabitants of the ocean. Microbes such as ''Alcanivorax'' provide a major route for the breakdown of these pollutants, and demonstrate how marine bacteria keep the environment in check. Of all the ''Alcanivorax'' species and other oil-degrading microbes, ''Alcanivorax borkumensis'' is one of the most important worldwide due to the fact it produces a wide variety of very efficient oil-degrading enzymes. With this knowledge, ''Alcanivorax borkumensis'' could provide a useful tool for bioremediation of oil spills.<br />
<br />
==Genome Structure==<br />
[[Image:Lorenzo.gif|thumbnail|200px|Figure 3. Mechanisms for oil degradation and survival encoded by the ''A. borkumensis SK2'' genome. Image from Victor de Lorenzo[http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html]]]<br />
<br />
The ''Alcanivorax borkumensis'' strain SK2 was the first hydrocarbonoclastic bacterium to be sequenced and was completed by Susanne Schneiker et al. It's genome consists of a single circular chromosome with 3,120,143 base pairs with an average G+C content of 54.7%. The genomic analysis of ''A. borkumensis SK2'' revealed several new insights into the bacterium's role for (i) n-alkane degradation (which includes metabolism, biosurfactant production and biofilm production), (ii) it's system for capturing or scavenging the small amounts of nitrogen, phosphorous, sulfur, and other elements in a nutrient-poor marine environment which allows for more efficient alkane degradation due to their main limitation of nutrient availability, (iii) as well as means for coping with stress factors such as high salt contents and high UV radiation since it thrives mostly in the upper layers in the ocean where UV light is encountered. It's genome encodes several systems for the catabolism of hydrocarbons which allow the bacertium to degrade all sorts of alkanes such as AlkB1 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C5-C12, and AlkB2 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C8 to C16. Both these systems are located close to the origin of replication of the chromosome. ''A. borkumensis'' is also able to degrade alkanes up to C32, branched aliphatic hydrocarbons, isoprenoid hydrocarbons such as phytane, as well as alkylarenes and alkylcycloalkanes. Thus, the genome encodes for a broad spectrum of systems for the catabolism of hydrocarbons, giving it a competitive advantage over other oil-degrading marine microbial communities. To deal with the damaging effects of UV light, ''A. borkumensis'' has a number of genes that reduce the damage. These include the full genes for DNA alkylation, recombinational and nucleotide excision repair, base excision repair, as well as the SOS response.<br />
<br />
==Cell Structure, Metabolism and Life Cycle==<br />
Interesting features of cell structure; how it gains energy; what important molecules it produces.<br />
<br />
<br />
==Ecology and Pathogenesis==<br />
Habitat; symbiosis; biogeochemical significance; contributions to environment.<br><br />
If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.<br><br><br />
<br />
==References==<br />
[1] [http://ijs.sgmjournals.org/cgi/content/abstract/53/1/331 Fernandez-Martinez, Javier, Maria J. Pujalte, Jesus Garcia-Martinez, Manuel Mata, Esperanza Garay, and Francisco Rodriguez-Valera. "Description of ''Alcanivorax Venustensis'' sp. nov. and Reclassification of ''Fundibacter Jadensis'' DSM 12178T (Bruns and Berthe-Corti 1999) As ''Alcanivorax Jadensis'' comb. nov., Members of the Emended Genus ''Alcanivorax''." International Journal of Systematic and Evolutionary Microbiology 53 (2003): 331-338.]<br />
<br />
[2] [http://www.blackwell-synergy.com/doi/pdf/10.1046/j.1468-2920.2003.00468.x Hara, Akihiro, Kazuaki Syutsubo, and Shigeaki Harayama. "''Alcanivorax'' Which Prevails In Oil-contaminated Seawater Exhibits Broad Substrate Specificity For Alkane Degradation." Environmental Microbiology 5.9 (2003): 746-753.]<br />
<br />
[3] [http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html Lorenzo, Víctor De. "Blueprint of an Oil-eating Bacterium." Nature Biotechnology 24 (2006): 952-953.]<br />
<br />
[4] [http://www.ncbi.nlm.nih.gov/pubmed/16878126 Schneiker, S. et al. "Genome Sequence of the Ubiquitous Hydrocarbon-degrading Marine Bacterium A''lcanivorax Borkumensis''." Nature Biotechnology 24 (2006): 997-1004.]<br />
<br />
[5] [http://ijs.sgmjournals.org/cgi/content/abstract/48/2/339 Yakimov, Michail M., Peter N. Golyshin, Siegmund Lang, Edward R. B. Moore, Wolf-Rainer Abraham, Heinrich Lunsdorf, and Kenneth N. Timmis. "''Alcanivorax Borkumensis'' gen. nov., sp. nov., A New, Hydrocarbon-degrading And Surfactant-producing Marine Bacterium." International Journal of Systematic Bacteriology 48 (1998): 339-348.]<br />
<br />
==Author==<br />
Page authored by Andrew Buss, student of [http://www.kbs.msu.edu/faculty/lennon/ Prof. Jay Lennon] at Michigan State University.</div>Bussand1https://microbewiki.kenyon.edu/index.php?title=Alcanivorax&diff=29716Alcanivorax2008-04-05T23:05:48Z<p>Bussand1: /* Genome Structure */</p>
<hr />
<div>[[Image:AlcVorax PROCARYOTES01b-1.JPG|thumbnail|300px|Figure 1. ''Alcanivorax borkumensis''. Image from Helmholtz Centre for Infection Research[http://www.helmholtz-hzi.de/en/news_public_relation/press_releases/view/article/complete/oil_tanker_accidents_as_a_source_of_food/]]]<br />
<br />
==Classification==<br />
<br />
Bacteria; Phylum: Proteobacteria; Class: Gammaproteobacteria; Order: Oceanospirillales; Family: Alcanivoracaceae<br />
<br />
===Species===<br />
<br />
{|<br />
| height="10" bgcolor="#FFDF95" |<br />
'''NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Tree&id=2&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy]'''<br />
|}<br />
<br />
*''Alcanivorax balearicum''<br />
*''Alcanivorax borkumensis''<br />
*''Alcanivorax dieselolei''<br />
*''Alcanivorax indicus''<br />
*''Alcanivorax jadensis''<br />
*''Alcanivorax venustensis''<br />
<br />
==Description and Significance==<br />
[[Image:PWS tanker oil spill.jpg|thumbnail|200px|Figure 2. Supertanker Exxon Valdez grounded on Bligh Reef which released 11 million gallons of crude oil into the water. This oil-contaminated seawater is the preferred habitat for ''Alcanivorax''. Image from USGS[http://menlocampus.wr.usgs.gov/50years/accomplishments/oil.html]]]<br />
<br />
''Alcanivorax'', first described in 1998, is a Gram-negative, aerobic, rod-shaped, oil-degrading marine bacterium that is found in low abundances in unpolluted environments in the upper layers of the ocean, but quickly becomes the predominant microbes in oil-contaminated open oceans and coastal waters when nitrogen and phosphorus are not limiting. When conditions in these moderately halophilic environments are right, ''Alcanivorax'' may make up 80-90% of the oil-degrading microbial community. As a result of their profound ability to degrade and live predominately on alkanes, as well as to become the dominant microbes in oil-contaminated areas, ''Alcanivorax'' plays a huge role in the biological cleanup of oil-contaminated environments. These oil-contaminated environments in the ocean are largely due to anthropogenic sources such as oil spills caused by tankers accidents, and cause serious ecological damage to coastal fauna and flora as well as other inhabitants of the ocean. Microbes such as ''Alcanivorax'' provide a major route for the breakdown of these pollutants, and demonstrate how marine bacteria keep the environment in check. Of all the ''Alcanivorax'' species and other oil-degrading microbes, ''Alcanivorax borkumensis'' is one of the most important worldwide due to the fact it produces a wide variety of very efficient oil-degrading enzymes. With this knowledge, ''Alcanivorax borkumensis'' could provide a useful tool for bioremediation of oil spills.<br />
<br />
==Genome Structure==<br />
[[Image:Lorenzo.gif|thumbnail|200px|Figure 3. Mechanisms for oil degradation and survival encoded by the ''A. borkumensis SK2'' genome. Image from Victor de Lorenzo[http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html]]]<br />
<br />
The ''Alcanivorax borkumensis'' strain SK2 was the first hydrocarbonoclastic bacterium to be sequenced and was completed by Susanne Schneiker et al. It's genome consists of a single circular chromosome with 3,120,143 base pairs with an average G+C content of 54.7%. The genomic analysis of ''A. borkumensis SK2'' revealed several new insights into the bacterium's role for (i) n-alkane degradation (which includes metabolism, biosurfactant production and biofilm production), (ii) it's system for capturing or scavenging the small amounts of nitrogen, phosphorous, sulfur, and other elements in a nutrient-poor marine environment which allows for more efficient alkane degradation due to their main limitation of nutrient availability, (iii) as well as means for coping with stress factors such as high salt contents and high UV radiation since it thrives mostly in the upper layers in the ocean where UV light is encountered. It's genome encodes several systems for the catabolism of hydrocarbons which allow the bacertium to degrade all sorts of alkanes such as AlkB1 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C5-C12, and AlkB2 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C8 to C16. Both these systems are located close to the origin of replication of the chromosome. ''A. borkumensis'' is also able to degrade alkanes up to C32, branched aliphatic hydrocarbons, isoprenoid hydrocarbons such as phytane, as well as alkylarenes and alkylcycloalkanes. Thus, the genome encodes for a broad spectrum of systems for the catabolism of hydrocarbons, giving it a competitive advantage over other oil-degrading marine microbial communities. To deal with the damaging effects of UV light, ''A. borkumensis'' has a number of genes that reduce the damage. These include the full genes for DNA alkylation, recombinational and nucleotide excision repair, base excision repair, as well as the SOS response.<br />
<br />
==Cell Structure, Metabolism and Life Cycle==<br />
Interesting features of cell structure; how it gains energy; what important molecules it produces.<br />
<br />
<br />
==Ecology and Pathogenesis==<br />
Habitat; symbiosis; biogeochemical significance; contributions to environment.<br><br />
If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.<br><br><br />
<br />
==References==<br />
[1] [http://ijs.sgmjournals.org/cgi/content/abstract/53/1/331 Fernandez-Martinez, Javier, Maria J. Pujalte, Jesus Garcia-Martinez, Manuel Mata, Esperanza Garay, and Francisco Rodriguez-Valera. "Description of ''Alcanivorax Venustensis'' sp. nov. and Reclassification of ''Fundibacter Jadensis'' DSM 12178T (Bruns and Berthe-Corti 1999) As ''Alcanivorax Jadensis'' comb. nov., Members of the Emended Genus ''Alcanivorax''." International Journal of Systematic and Evolutionary Microbiology 53 (2003): 331-338.]<br />
<br />
[2] [http://www.blackwell-synergy.com/doi/pdf/10.1046/j.1468-2920.2003.00468.x Hara, Akihiro, Kazuaki Syutsubo, and Shigeaki Harayama. "''Alcanivorax'' Which Prevails In Oil-contaminated Seawater Exhibits Broad Substrate Specificity For Alkane Degradation." Environmental Microbiology 5.9 (2003): 746-753.]<br />
<br />
[3] [http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html Lorenzo, Víctor De. "Blueprint of an Oil-eating Bacterium." Nature Biotechnology 24 (2006): 952-953.]<br />
<br />
[4] [http://www.ncbi.nlm.nih.gov/pubmed/16878126 Schneiker, S. et al. "Genome Sequence of the Ubiquitous Hydrocarbon-degrading Marine Bacterium A''lcanivorax Borkumensis''." Nature Biotechnology 24 (2006): 997-1004.]<br />
<br />
[5] [http://ijs.sgmjournals.org/cgi/content/abstract/48/2/339 Yakimov, Michail M., Peter N. Golyshin, Siegmund Lang, Edward R. B. Moore, Wolf-Rainer Abraham, Heinrich Lunsdorf, and Kenneth N. Timmis. "''Alcanivorax Borkumensis'' gen. nov., sp. nov., A New, Hydrocarbon-degrading And Surfactant-producing Marine Bacterium." International Journal of Systematic Bacteriology 48 (1998): 339-348.]<br />
<br />
==Author==<br />
Page authored by Andrew Buss, student of [http://www.kbs.msu.edu/faculty/lennon/ Prof. Jay Lennon] at Michigan State University.</div>Bussand1https://microbewiki.kenyon.edu/index.php?title=File:Lorenzo.gif&diff=29715File:Lorenzo.gif2008-04-05T23:05:10Z<p>Bussand1: </p>
<hr />
<div>Mechanisms for oil degradation and survival encoded by the ''A. borkumensis SK2'' genome.</div>Bussand1https://microbewiki.kenyon.edu/index.php?title=File:Lorenzo.gif&diff=29714File:Lorenzo.gif2008-04-05T23:04:54Z<p>Bussand1: </p>
<hr />
<div>Mechanisms for oil degradation and survival encoded by the A. borkumensis SK2 genome.</div>Bussand1https://microbewiki.kenyon.edu/index.php?title=Alcanivorax&diff=29713Alcanivorax2008-04-05T22:57:38Z<p>Bussand1: /* Genome Structure */</p>
<hr />
<div>[[Image:AlcVorax PROCARYOTES01b-1.JPG|thumbnail|300px|Figure 1. ''Alcanivorax borkumensis''. Image from Helmholtz Centre for Infection Research[http://www.helmholtz-hzi.de/en/news_public_relation/press_releases/view/article/complete/oil_tanker_accidents_as_a_source_of_food/]]]<br />
<br />
==Classification==<br />
<br />
Bacteria; Phylum: Proteobacteria; Class: Gammaproteobacteria; Order: Oceanospirillales; Family: Alcanivoracaceae<br />
<br />
===Species===<br />
<br />
{|<br />
| height="10" bgcolor="#FFDF95" |<br />
'''NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Tree&id=2&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy]'''<br />
|}<br />
<br />
*''Alcanivorax balearicum''<br />
*''Alcanivorax borkumensis''<br />
*''Alcanivorax dieselolei''<br />
*''Alcanivorax indicus''<br />
*''Alcanivorax jadensis''<br />
*''Alcanivorax venustensis''<br />
<br />
==Description and Significance==<br />
[[Image:PWS tanker oil spill.jpg|thumbnail|200px|Figure 2. Supertanker Exxon Valdez grounded on Bligh Reef which released 11 million gallons of crude oil into the water. This oil-contaminated seawater is the preferred habitat for ''Alcanivorax''. Image from USGS[http://menlocampus.wr.usgs.gov/50years/accomplishments/oil.html]]]<br />
<br />
''Alcanivorax'', first described in 1998, is a Gram-negative, aerobic, rod-shaped, oil-degrading marine bacterium that is found in low abundances in unpolluted environments in the upper layers of the ocean, but quickly becomes the predominant microbes in oil-contaminated open oceans and coastal waters when nitrogen and phosphorus are not limiting. When conditions in these moderately halophilic environments are right, ''Alcanivorax'' may make up 80-90% of the oil-degrading microbial community. As a result of their profound ability to degrade and live predominately on alkanes, as well as to become the dominant microbes in oil-contaminated areas, ''Alcanivorax'' plays a huge role in the biological cleanup of oil-contaminated environments. These oil-contaminated environments in the ocean are largely due to anthropogenic sources such as oil spills caused by tankers accidents, and cause serious ecological damage to coastal fauna and flora as well as other inhabitants of the ocean. Microbes such as ''Alcanivorax'' provide a major route for the breakdown of these pollutants, and demonstrate how marine bacteria keep the environment in check. Of all the ''Alcanivorax'' species and other oil-degrading microbes, ''Alcanivorax borkumensis'' is one of the most important worldwide due to the fact it produces a wide variety of very efficient oil-degrading enzymes. With this knowledge, ''Alcanivorax borkumensis'' could provide a useful tool for bioremediation of oil spills.<br />
<br />
==Genome Structure==<br />
[[Image:Lorenzo.gif|thumbnail|200px|Figure 3. Some of the mechanisms for oil degradation and survival encoded by the ''A. borkumensis SK2'' genome. Image from Victor de Lorenzo[http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html]]]<br />
<br />
The ''Alcanivorax borkumensis'' strain SK2 was the first hydrocarbonoclastic bacterium to be sequenced and was completed by Susanne Schneiker et al. It's genome consists of a single circular chromosome with 3,120,143 base pairs with an average G+C content of 54.7%. The genomic analysis of ''A. borkumensis SK2'' revealed several new insights into the bacterium's role for (i) n-alkane degradation (which includes metabolism, biosurfactant production and biofilm production), (ii) it's system for capturing or scavenging the small amounts of nitrogen, phosphorous, sulfur, and other elements in a nutrient-poor marine environment which allows for more efficient alkane degradation due to their main limitation of nutrient availability, (iii) as well as means for coping with stress factors such as high salt contents and high UV radiation since it thrives mostly in the upper layers in the ocean where UV light is encountered. It's genome encodes several systems for the catabolism of hydrocarbons which allow the bacertium to degrade all sorts of alkanes such as AlkB1 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C5-C12, and AlkB2 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C8 to C16. Both these systems are located close to the origin of replication of the chromosome. ''A. borkumensis'' is also able to degrade alkanes up to C32, branched aliphatic hydrocarbons, isoprenoid hydrocarbons such as phytane, as well as alkylarenes and alkylcycloalkanes. Thus, the genome encodes for a broad spectrum of systems for the catabolism of hydrocarbons, giving it a competitive advantage over other oil-degrading marine microbial communities. To deal with the damaging effects of UV light, ''A. borkumensis'' has a number of genes that reduce the damage. These include the full genes for DNA alkylation, recombinational and nucleotide excision repair, base excision repair, as well as the SOS response.<br />
<br />
==Cell Structure, Metabolism and Life Cycle==<br />
Interesting features of cell structure; how it gains energy; what important molecules it produces.<br />
<br />
<br />
==Ecology and Pathogenesis==<br />
Habitat; symbiosis; biogeochemical significance; contributions to environment.<br><br />
If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.<br><br><br />
<br />
==References==<br />
[1] [http://ijs.sgmjournals.org/cgi/content/abstract/53/1/331 Fernandez-Martinez, Javier, Maria J. Pujalte, Jesus Garcia-Martinez, Manuel Mata, Esperanza Garay, and Francisco Rodriguez-Valera. "Description of ''Alcanivorax Venustensis'' sp. nov. and Reclassification of ''Fundibacter Jadensis'' DSM 12178T (Bruns and Berthe-Corti 1999) As ''Alcanivorax Jadensis'' comb. nov., Members of the Emended Genus ''Alcanivorax''." International Journal of Systematic and Evolutionary Microbiology 53 (2003): 331-338.]<br />
<br />
[2] [http://www.blackwell-synergy.com/doi/pdf/10.1046/j.1468-2920.2003.00468.x Hara, Akihiro, Kazuaki Syutsubo, and Shigeaki Harayama. "''Alcanivorax'' Which Prevails In Oil-contaminated Seawater Exhibits Broad Substrate Specificity For Alkane Degradation." Environmental Microbiology 5.9 (2003): 746-753.]<br />
<br />
[3] [http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html Lorenzo, Víctor De. "Blueprint of an Oil-eating Bacterium." Nature Biotechnology 24 (2006): 952-953.]<br />
<br />
[4] [http://www.ncbi.nlm.nih.gov/pubmed/16878126 Schneiker, S. et al. "Genome Sequence of the Ubiquitous Hydrocarbon-degrading Marine Bacterium A''lcanivorax Borkumensis''." Nature Biotechnology 24 (2006): 997-1004.]<br />
<br />
[5] [http://ijs.sgmjournals.org/cgi/content/abstract/48/2/339 Yakimov, Michail M., Peter N. Golyshin, Siegmund Lang, Edward R. B. Moore, Wolf-Rainer Abraham, Heinrich Lunsdorf, and Kenneth N. Timmis. "''Alcanivorax Borkumensis'' gen. nov., sp. nov., A New, Hydrocarbon-degrading And Surfactant-producing Marine Bacterium." International Journal of Systematic Bacteriology 48 (1998): 339-348.]<br />
<br />
==Author==<br />
Page authored by Andrew Buss, student of [http://www.kbs.msu.edu/faculty/lennon/ Prof. Jay Lennon] at Michigan State University.</div>Bussand1https://microbewiki.kenyon.edu/index.php?title=Alcanivorax&diff=29712Alcanivorax2008-04-05T22:49:09Z<p>Bussand1: /* Description and Significance */</p>
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<div>[[Image:AlcVorax PROCARYOTES01b-1.JPG|thumbnail|300px|Figure 1. ''Alcanivorax borkumensis''. Image from Helmholtz Centre for Infection Research[http://www.helmholtz-hzi.de/en/news_public_relation/press_releases/view/article/complete/oil_tanker_accidents_as_a_source_of_food/]]]<br />
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==Classification==<br />
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Bacteria; Phylum: Proteobacteria; Class: Gammaproteobacteria; Order: Oceanospirillales; Family: Alcanivoracaceae<br />
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===Species===<br />
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{|<br />
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'''NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Tree&id=2&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy]'''<br />
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*''Alcanivorax balearicum''<br />
*''Alcanivorax borkumensis''<br />
*''Alcanivorax dieselolei''<br />
*''Alcanivorax indicus''<br />
*''Alcanivorax jadensis''<br />
*''Alcanivorax venustensis''<br />
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==Description and Significance==<br />
[[Image:PWS tanker oil spill.jpg|thumbnail|200px|Figure 2. Supertanker Exxon Valdez grounded on Bligh Reef which released 11 million gallons of crude oil into the water. This oil-contaminated seawater is the preferred habitat for ''Alcanivorax''. Image from USGS[http://menlocampus.wr.usgs.gov/50years/accomplishments/oil.html]]]<br />
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''Alcanivorax'', first described in 1998, is a Gram-negative, aerobic, rod-shaped, oil-degrading marine bacterium that is found in low abundances in unpolluted environments in the upper layers of the ocean, but quickly becomes the predominant microbes in oil-contaminated open oceans and coastal waters when nitrogen and phosphorus are not limiting. When conditions in these moderately halophilic environments are right, ''Alcanivorax'' may make up 80-90% of the oil-degrading microbial community. As a result of their profound ability to degrade and live predominately on alkanes, as well as to become the dominant microbes in oil-contaminated areas, ''Alcanivorax'' plays a huge role in the biological cleanup of oil-contaminated environments. These oil-contaminated environments in the ocean are largely due to anthropogenic sources such as oil spills caused by tankers accidents, and cause serious ecological damage to coastal fauna and flora as well as other inhabitants of the ocean. Microbes such as ''Alcanivorax'' provide a major route for the breakdown of these pollutants, and demonstrate how marine bacteria keep the environment in check. Of all the ''Alcanivorax'' species and other oil-degrading microbes, ''Alcanivorax borkumensis'' is one of the most important worldwide due to the fact it produces a wide variety of very efficient oil-degrading enzymes. With this knowledge, ''Alcanivorax borkumensis'' could provide a useful tool for bioremediation of oil spills.<br />
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==Genome Structure==<br />
[[Image:Lorenzo.gif|thumbnail|200px|Figure 3. Some of the mechanisms for oil degradation and survival encoded by the ''A. borkumensis SK2'' genome. Image from Victor de Lorenzo[http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html]]]<br />
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The ''Alcanivorax borkumensis'' strain SK2 was the first hydrocarbonoclastic bacterium to be sequenced and was completed by Susanne Schneiker et al. It's genome consists of a single circular chromosome with 3,120,143 base pairs with an average G+C content of 54.7%. The genomic analysis of ''A. borkumensis SK2'' revealed several new insights into the bacterium's role for (i) n-alkane degradation (which includes metabolism, biosurfactant production and biofilm production), (ii) it's system for capturing or scavenging the small amounts of nitrogen, phosphorous, sulfur, and other elements in a nutrient-poor marine environment which allows for more efficient alkane degradation due to their main limitation of nutrient availability, (iii) as well as means for coping with stress factors such as high salt contents and high UV radiation since it thrives mostly in the upper layers in the ocean. It's genome encodes several systems for the catabolism of hydrocarbons which allow the bacertium to degrade all sorts of alkanes such as AlkB1 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C5-C12, and AlkB2 alkane hydroxylase which oxidizes medium-chain alkanes in the range of C8 to C16. Both these systems are located close to the origin of replication of the chromosome. ''A. borkumensis'' is also able to degrade alkanes up to C32, branched aliphatic hydrocarbons, isoprenoid hydrocarbons such as phytane, as well as alkylarenes and alkylcycloalkanes. Thus, the genome encodes for a broad spectrum of systems for the catabolism of hydrocarbons, giving it a competitive advantage over other oil-degrading marine microbial communities.<br />
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==Cell Structure, Metabolism and Life Cycle==<br />
Interesting features of cell structure; how it gains energy; what important molecules it produces.<br />
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==Ecology and Pathogenesis==<br />
Habitat; symbiosis; biogeochemical significance; contributions to environment.<br><br />
If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.<br><br><br />
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==References==<br />
[1] [http://ijs.sgmjournals.org/cgi/content/abstract/53/1/331 Fernandez-Martinez, Javier, Maria J. Pujalte, Jesus Garcia-Martinez, Manuel Mata, Esperanza Garay, and Francisco Rodriguez-Valera. "Description of ''Alcanivorax Venustensis'' sp. nov. and Reclassification of ''Fundibacter Jadensis'' DSM 12178T (Bruns and Berthe-Corti 1999) As ''Alcanivorax Jadensis'' comb. nov., Members of the Emended Genus ''Alcanivorax''." International Journal of Systematic and Evolutionary Microbiology 53 (2003): 331-338.]<br />
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[2] [http://www.blackwell-synergy.com/doi/pdf/10.1046/j.1468-2920.2003.00468.x Hara, Akihiro, Kazuaki Syutsubo, and Shigeaki Harayama. "''Alcanivorax'' Which Prevails In Oil-contaminated Seawater Exhibits Broad Substrate Specificity For Alkane Degradation." Environmental Microbiology 5.9 (2003): 746-753.]<br />
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[3] [http://www.nature.com/nbt/journal/v24/n8/full/nbt0806-952.html Lorenzo, Víctor De. "Blueprint of an Oil-eating Bacterium." Nature Biotechnology 24 (2006): 952-953.]<br />
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[4] [http://www.ncbi.nlm.nih.gov/pubmed/16878126 Schneiker, S. et al. "Genome Sequence of the Ubiquitous Hydrocarbon-degrading Marine Bacterium A''lcanivorax Borkumensis''." Nature Biotechnology 24 (2006): 997-1004.]<br />
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[5] [http://ijs.sgmjournals.org/cgi/content/abstract/48/2/339 Yakimov, Michail M., Peter N. Golyshin, Siegmund Lang, Edward R. B. Moore, Wolf-Rainer Abraham, Heinrich Lunsdorf, and Kenneth N. Timmis. "''Alcanivorax Borkumensis'' gen. nov., sp. nov., A New, Hydrocarbon-degrading And Surfactant-producing Marine Bacterium." International Journal of Systematic Bacteriology 48 (1998): 339-348.]<br />
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==Author==<br />
Page authored by Andrew Buss, student of [http://www.kbs.msu.edu/faculty/lennon/ Prof. Jay Lennon] at Michigan State University.</div>Bussand1