https://microbewiki.kenyon.edu/api.php?action=feedcontributions&user=Suchanart.apisaksiri&feedformat=atommicrobewiki - User contributions [en]2024-03-28T23:17:34ZUser contributionsMediaWiki 1.39.6https://microbewiki.kenyon.edu/index.php?title=NS_Mystery_Organism&diff=132329NS Mystery Organism2017-12-08T20:49:26Z<p>Suchanart.apisaksiri: /* Genome Structure */</p>
<hr />
<div>{{Uncurated}}<br />
==Classification==<br />
<br />
Domain; Phylum; Class; Order; family [Others may be used. Use [http://www.ncbi.nlm.nih.gov/Taxonomy/ NCBI] link to find]<br />
<br />
Unknown <br />
<br />
===Species===<br />
<br />
{|<br />
| height="10" bgcolor="#FFDF95" |<br />
'''NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Tree&id=2&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy]'''<br />
|}<br />
<br />
''NS Mystery Organism''<br />
<br />
==Habitat Information ==<br />
Describe the location and conditions under which the organism was isolated.<br />
<br />
Date: 09/06/2017 <br />
<br />
Time: 18:49:00 CST<br />
<br />
Air Temperature: 83 ℉<br />
<br />
Humidity: 33%<br />
<br />
24 hr rainfall: 0 inches<br />
<br />
Pressure: 30.6 inch/ 1,017.0 mb<br />
<br />
Solar Radiation: 22.49 ms/m²<br />
<br />
Latitude: 30.144673 | Longitude: -97.841463<br />
<br />
The organism was isolated at a latitude of 30.144673 and a longitude of -97.841463 on September 6, 2017 at 18:49:00 CST. The conditions under which the NS Mystery Organism was isolated includes it being 83 degrees fahrenheit and 33% humidity. There were 0 inches of rainfall with a solar radiation of 22.49 ms/m^2.<br />
<br />
==Description and Significance==<br />
<br />
The colony morphology of NS Mystery Organism has a smooth surface with slightly raised elevation and an entire margin. The organism exhibits creamy yellow color. <br />
<br />
Cellular arrangement through microscopic observation is Diplobacilli. <br />
<br />
'''Significance'''<br />
<br />
The antimicrobial activity test has shown a zone of inhibitions with all the antibiotics used which are listed below.<br />
Antimicrobial agents used: <br />
1) Sulbactam,<br />
2. Ticracillin<br />
3. Cefoxitin<br />
4. Oxacillin <br />
5. Vancomycin<br />
<br />
The Organism was also sensitized by some disinfectants used including Tea tree, PepperMint, Lysol 10% and Bleach 100%.<br />
<br />
==Genome Structure==<br />
<br />
PCR and Gel electrophoresis tests were inconclusive, therefore, the results are unknown due to complications from sequence reactions.<br />
<br />
==Cell Structure, Metabolism and Life Cycle==<br />
<br />
'''Gram Stain''' NS Mystery Organism shows to be Gram-positive under Gram stain reaction test with prominent purple color from Crystal Violet. <br />
<br />
'''Capsule Stain''' Under Capsule Stain test, the result is negative without any appearance of Capsule. <br />
<br />
'''Endospore test''' The Organism also shows Negative Endospore test result under Endospore Stain test. <br />
<br />
'''Motility test''' However, under the Motility test, there was a movement away from the stab line into the medium which indicates a Positive Motility test result. Non-motile organisms which lack flagella, are usually going to form a single line of growth that does not spread into the surrounding area. While a motile bacterium will grow and make a hazy zone around the stab line. <br />
<br />
'''Oxidase test''' The NS Mystery Organism has shown a Positive test result which indicates Aerobic respiration. It can use oxygen as a terminal electron acceptor in respiration which was proven through a positive oxidase test. <br />
<br />
'''Triple Sugar Iron Agar''' NS Mystery Organism turns yellow/yellow under Triple Sugar Iron Agar (TSI) test points out fermentation of Lactose and Glucose.<br />
<br />
'''DNA Hydrolysis''' The Organism produces DNase enzyme under DNA hydrolysis test with a clearing around growth in the agar plate. <br />
<br />
'''Lipid Hydrolysis''' It also shows to have Lipase enzyme with a weak positive test result of Lipid Hydrolysis.<br />
<br />
==Physiology and Pathogenesis==<br />
<br />
'''Mannitol Salt Agar''' The Mannitol Salt Agar result for the organism indicated a positive result. The medium shows good yellow growth and is presumed Staphylococci because it is not inhibited by NaCl. The organism produces acid from Mannitol fermentation and is presumed possible pathogenic Staphylococcus aureus. <br />
<br />
'''Lipid Hydrolysis''' Lipid hydrolysis was used to detect lipolytic bacteria, especially in high-fat dairy products. Lipase allows the organisms that produce it to break down lipids into smaller fragments. The test showed a weak positive.<br />
<br />
'''Starch Hydrolysis''' Results : Inconclusive/Unknown<br />
<br />
'''Phenol Red Broth''': Results : Negative Fermentation.<br />
<br />
'''Casein Hydrolysis''': Results : Negative. There was no clearing around the organism which indicates a negative test result. <br />
<br />
'''Gelatin Hydrolysis''': Results : Negative. The organism shows inability to produces Gelatinase. <br />
<br />
'''Eosin methylene blue agar''' EMB test is used to encourage the growth of fecal coliforms, to test drinking water. The result is based on lactose fermentation. The results were positive since pink colonies formed, indicating slow and weak fermentation.<br />
<br />
'''Phenylethyl Alcohol Agar''' The organism is not inhibited by phenylethyl alcohol as proven with Phenylethyl Alcohol Agar.<br />
<br />
==References==<br />
[Sample reference] [http://ijs.sgmjournals.org/cgi/reprint/50/2/489 Takai, K., Sugai, A., Itoh, T., and Horikoshi, K. "''Palaeococcus ferrophilus'' gen. nov., sp. nov., a barophilic, hyperthermophilic archaeon from a deep-sea hydrothermal vent chimney". ''International Journal of Systematic and Evolutionary Microbiology''. 2000. Volume 50. p. 489-500.]<br />
<br />
==Author==<br />
Page authored by Suchanart Apisaksiri and Sally Phonthaasa, students of Prof. Kristine Hollingsworth at Austin Community College.<br />
<br />
<!-- Do not remove this line-->[[Category:Pages edited by students of Kristine Hollingsworth at Austin Community College]]</div>Suchanart.apisaksirihttps://microbewiki.kenyon.edu/index.php?title=NS_Mystery_Organism&diff=132328NS Mystery Organism2017-12-08T20:47:46Z<p>Suchanart.apisaksiri: /* Cell Structure, Metabolism and Life Cycle */</p>
<hr />
<div>{{Uncurated}}<br />
==Classification==<br />
<br />
Domain; Phylum; Class; Order; family [Others may be used. Use [http://www.ncbi.nlm.nih.gov/Taxonomy/ NCBI] link to find]<br />
<br />
Unknown <br />
<br />
===Species===<br />
<br />
{|<br />
| height="10" bgcolor="#FFDF95" |<br />
'''NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Tree&id=2&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy]'''<br />
|}<br />
<br />
''NS Mystery Organism''<br />
<br />
==Habitat Information ==<br />
Describe the location and conditions under which the organism was isolated.<br />
<br />
Date: 09/06/2017 <br />
<br />
Time: 18:49:00 CST<br />
<br />
Air Temperature: 83 ℉<br />
<br />
Humidity: 33%<br />
<br />
24 hr rainfall: 0 inches<br />
<br />
Pressure: 30.6 inch/ 1,017.0 mb<br />
<br />
Solar Radiation: 22.49 ms/m²<br />
<br />
Latitude: 30.144673 | Longitude: -97.841463<br />
<br />
The organism was isolated at a latitude of 30.144673 and a longitude of -97.841463 on September 6, 2017 at 18:49:00 CST. The conditions under which the NS Mystery Organism was isolated includes it being 83 degrees fahrenheit and 33% humidity. There were 0 inches of rainfall with a solar radiation of 22.49 ms/m^2.<br />
<br />
==Description and Significance==<br />
<br />
The colony morphology of NS Mystery Organism has a smooth surface with slightly raised elevation and an entire margin. The organism exhibits creamy yellow color. <br />
<br />
Cellular arrangement through microscopic observation is Diplobacilli. <br />
<br />
'''Significance'''<br />
<br />
The antimicrobial activity test has shown a zone of inhibitions with all the antibiotics used which are listed below.<br />
Antimicrobial agents used: <br />
1) Sulbactam,<br />
2. Ticracillin<br />
3. Cefoxitin<br />
4. Oxacillin <br />
5. Vancomycin<br />
<br />
The Organism was also sensitized by some disinfectants used including Tea tree, PepperMint, Lysol 10% and Bleach 100%.<br />
<br />
==Genome Structure==<br />
<br />
Unknown due to complications from sequence reactions.<br />
<br />
==Cell Structure, Metabolism and Life Cycle==<br />
<br />
'''Gram Stain''' NS Mystery Organism shows to be Gram-positive under Gram stain reaction test with prominent purple color from Crystal Violet. <br />
<br />
'''Capsule Stain''' Under Capsule Stain test, the result is negative without any appearance of Capsule. <br />
<br />
'''Endospore test''' The Organism also shows Negative Endospore test result under Endospore Stain test. <br />
<br />
'''Motility test''' However, under the Motility test, there was a movement away from the stab line into the medium which indicates a Positive Motility test result. Non-motile organisms which lack flagella, are usually going to form a single line of growth that does not spread into the surrounding area. While a motile bacterium will grow and make a hazy zone around the stab line. <br />
<br />
'''Oxidase test''' The NS Mystery Organism has shown a Positive test result which indicates Aerobic respiration. It can use oxygen as a terminal electron acceptor in respiration which was proven through a positive oxidase test. <br />
<br />
'''Triple Sugar Iron Agar''' NS Mystery Organism turns yellow/yellow under Triple Sugar Iron Agar (TSI) test points out fermentation of Lactose and Glucose.<br />
<br />
'''DNA Hydrolysis''' The Organism produces DNase enzyme under DNA hydrolysis test with a clearing around growth in the agar plate. <br />
<br />
'''Lipid Hydrolysis''' It also shows to have Lipase enzyme with a weak positive test result of Lipid Hydrolysis.<br />
<br />
==Physiology and Pathogenesis==<br />
<br />
'''Mannitol Salt Agar''' The Mannitol Salt Agar result for the organism indicated a positive result. The medium shows good yellow growth and is presumed Staphylococci because it is not inhibited by NaCl. The organism produces acid from Mannitol fermentation and is presumed possible pathogenic Staphylococcus aureus. <br />
<br />
'''Lipid Hydrolysis''' Lipid hydrolysis was used to detect lipolytic bacteria, especially in high-fat dairy products. Lipase allows the organisms that produce it to break down lipids into smaller fragments. The test showed a weak positive.<br />
<br />
'''Starch Hydrolysis''' Results : Inconclusive/Unknown<br />
<br />
'''Phenol Red Broth''': Results : Negative Fermentation.<br />
<br />
'''Casein Hydrolysis''': Results : Negative. There was no clearing around the organism which indicates a negative test result. <br />
<br />
'''Gelatin Hydrolysis''': Results : Negative. The organism shows inability to produces Gelatinase. <br />
<br />
'''Eosin methylene blue agar''' EMB test is used to encourage the growth of fecal coliforms, to test drinking water. The result is based on lactose fermentation. The results were positive since pink colonies formed, indicating slow and weak fermentation.<br />
<br />
'''Phenylethyl Alcohol Agar''' The organism is not inhibited by phenylethyl alcohol as proven with Phenylethyl Alcohol Agar.<br />
<br />
==References==<br />
[Sample reference] [http://ijs.sgmjournals.org/cgi/reprint/50/2/489 Takai, K., Sugai, A., Itoh, T., and Horikoshi, K. "''Palaeococcus ferrophilus'' gen. nov., sp. nov., a barophilic, hyperthermophilic archaeon from a deep-sea hydrothermal vent chimney". ''International Journal of Systematic and Evolutionary Microbiology''. 2000. Volume 50. p. 489-500.]<br />
<br />
==Author==<br />
Page authored by Suchanart Apisaksiri and Sally Phonthaasa, students of Prof. Kristine Hollingsworth at Austin Community College.<br />
<br />
<!-- Do not remove this line-->[[Category:Pages edited by students of Kristine Hollingsworth at Austin Community College]]</div>Suchanart.apisaksirihttps://microbewiki.kenyon.edu/index.php?title=NS_Mystery_Organism&diff=132327NS Mystery Organism2017-12-08T20:43:03Z<p>Suchanart.apisaksiri: /* Physiology and Pathogenesis */</p>
<hr />
<div>{{Uncurated}}<br />
==Classification==<br />
<br />
Domain; Phylum; Class; Order; family [Others may be used. Use [http://www.ncbi.nlm.nih.gov/Taxonomy/ NCBI] link to find]<br />
<br />
Unknown <br />
<br />
===Species===<br />
<br />
{|<br />
| height="10" bgcolor="#FFDF95" |<br />
'''NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Tree&id=2&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy]'''<br />
|}<br />
<br />
''NS Mystery Organism''<br />
<br />
==Habitat Information ==<br />
Describe the location and conditions under which the organism was isolated.<br />
<br />
Date: 09/06/2017 <br />
<br />
Time: 18:49:00 CST<br />
<br />
Air Temperature: 83 ℉<br />
<br />
Humidity: 33%<br />
<br />
24 hr rainfall: 0 inches<br />
<br />
Pressure: 30.6 inch/ 1,017.0 mb<br />
<br />
Solar Radiation: 22.49 ms/m²<br />
<br />
Latitude: 30.144673 | Longitude: -97.841463<br />
<br />
The organism was isolated at a latitude of 30.144673 and a longitude of -97.841463 on September 6, 2017 at 18:49:00 CST. The conditions under which the NS Mystery Organism was isolated includes it being 83 degrees fahrenheit and 33% humidity. There were 0 inches of rainfall with a solar radiation of 22.49 ms/m^2.<br />
<br />
==Description and Significance==<br />
<br />
The colony morphology of NS Mystery Organism has a smooth surface with slightly raised elevation and an entire margin. The organism exhibits creamy yellow color. <br />
<br />
Cellular arrangement through microscopic observation is Diplobacilli. <br />
<br />
'''Significance'''<br />
<br />
The antimicrobial activity test has shown a zone of inhibitions with all the antibiotics used which are listed below.<br />
Antimicrobial agents used: <br />
1) Sulbactam,<br />
2. Ticracillin<br />
3. Cefoxitin<br />
4. Oxacillin <br />
5. Vancomycin<br />
<br />
The Organism was also sensitized by some disinfectants used including Tea tree, PepperMint, Lysol 10% and Bleach 100%.<br />
<br />
==Genome Structure==<br />
<br />
Unknown due to complications from sequence reactions.<br />
<br />
==Cell Structure, Metabolism and Life Cycle==<br />
<br />
'''Gram Stain''' NS Mystery Organism shows to be Gram-positive under Gram stain reaction test with prominent purple color from Crystal Violet. <br />
<br />
'''Capsule Stain''' Under Capsule Stain test, the result is negative without any appearance of Capsule. <br />
<br />
'''Endospore test''' The Organism also shows Negative Endospore test result under Endospore Stain test. <br />
<br />
'''Motility test''' However, under the Motility test, there was a movement away from the stab line into the medium which indicates a Positive Motility test result. Non-motile organisms which lack flagella, are usually going to form a single line of growth that does not spread into the surrounding area. While a motile bacterium will grow and make a hazy zone around the stab line. <br />
<br />
'''Oxidase test''' Oxidase test has shown Positive result indicating Aerobic respiration.<br />
<br />
'''Triple Sugar Iron Agar''' NS Mystery Organism turns yellow/yellow under Triple Sugar Iron Agar (TSI) test points out fermentation of Lactose and Glucose.<br />
<br />
'''DNA Hydrolysis''' The Organism produces DNase enzyme under DNA hydrolysis test with a clearing around growth in the agar plate. <br />
<br />
'''Lipid Hydrolysis''' It also shows to have Lipase enzyme with a weak positive test result of Lipid Hydrolysis.<br />
<br />
==Physiology and Pathogenesis==<br />
<br />
'''Mannitol Salt Agar''' The Mannitol Salt Agar result for the organism indicated a positive result. The medium shows good yellow growth and is presumed Staphylococci because it is not inhibited by NaCl. The organism produces acid from Mannitol fermentation and is presumed possible pathogenic Staphylococcus aureus. <br />
<br />
'''Lipid Hydrolysis''' Lipid hydrolysis was used to detect lipolytic bacteria, especially in high-fat dairy products. Lipase allows the organisms that produce it to break down lipids into smaller fragments. The test showed a weak positive.<br />
<br />
'''Starch Hydrolysis''' Results : Inconclusive/Unknown<br />
<br />
'''Phenol Red Broth''': Results : Negative Fermentation.<br />
<br />
'''Casein Hydrolysis''': Results : Negative. There was no clearing around the organism which indicates a negative test result. <br />
<br />
'''Gelatin Hydrolysis''': Results : Negative. The organism shows inability to produces Gelatinase. <br />
<br />
'''Eosin methylene blue agar''' EMB test is used to encourage the growth of fecal coliforms, to test drinking water. The result is based on lactose fermentation. The results were positive since pink colonies formed, indicating slow and weak fermentation.<br />
<br />
'''Phenylethyl Alcohol Agar''' The organism is not inhibited by phenylethyl alcohol as proven with Phenylethyl Alcohol Agar.<br />
<br />
==References==<br />
[Sample reference] [http://ijs.sgmjournals.org/cgi/reprint/50/2/489 Takai, K., Sugai, A., Itoh, T., and Horikoshi, K. "''Palaeococcus ferrophilus'' gen. nov., sp. nov., a barophilic, hyperthermophilic archaeon from a deep-sea hydrothermal vent chimney". ''International Journal of Systematic and Evolutionary Microbiology''. 2000. Volume 50. p. 489-500.]<br />
<br />
==Author==<br />
Page authored by Suchanart Apisaksiri and Sally Phonthaasa, students of Prof. Kristine Hollingsworth at Austin Community College.<br />
<br />
<!-- Do not remove this line-->[[Category:Pages edited by students of Kristine Hollingsworth at Austin Community College]]</div>Suchanart.apisaksirihttps://microbewiki.kenyon.edu/index.php?title=NS_Mystery_Organism&diff=132326NS Mystery Organism2017-12-08T20:41:39Z<p>Suchanart.apisaksiri: /* Physiology and Pathogenesis */</p>
<hr />
<div>{{Uncurated}}<br />
==Classification==<br />
<br />
Domain; Phylum; Class; Order; family [Others may be used. Use [http://www.ncbi.nlm.nih.gov/Taxonomy/ NCBI] link to find]<br />
<br />
Unknown <br />
<br />
===Species===<br />
<br />
{|<br />
| height="10" bgcolor="#FFDF95" |<br />
'''NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Tree&id=2&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy]'''<br />
|}<br />
<br />
''NS Mystery Organism''<br />
<br />
==Habitat Information ==<br />
Describe the location and conditions under which the organism was isolated.<br />
<br />
Date: 09/06/2017 <br />
<br />
Time: 18:49:00 CST<br />
<br />
Air Temperature: 83 ℉<br />
<br />
Humidity: 33%<br />
<br />
24 hr rainfall: 0 inches<br />
<br />
Pressure: 30.6 inch/ 1,017.0 mb<br />
<br />
Solar Radiation: 22.49 ms/m²<br />
<br />
Latitude: 30.144673 | Longitude: -97.841463<br />
<br />
The organism was isolated at a latitude of 30.144673 and a longitude of -97.841463 on September 6, 2017 at 18:49:00 CST. The conditions under which the NS Mystery Organism was isolated includes it being 83 degrees fahrenheit and 33% humidity. There were 0 inches of rainfall with a solar radiation of 22.49 ms/m^2.<br />
<br />
==Description and Significance==<br />
<br />
The colony morphology of NS Mystery Organism has a smooth surface with slightly raised elevation and an entire margin. The organism exhibits creamy yellow color. <br />
<br />
Cellular arrangement through microscopic observation is Diplobacilli. <br />
<br />
'''Significance'''<br />
<br />
The antimicrobial activity test has shown a zone of inhibitions with all the antibiotics used which are listed below.<br />
Antimicrobial agents used: <br />
1) Sulbactam,<br />
2. Ticracillin<br />
3. Cefoxitin<br />
4. Oxacillin <br />
5. Vancomycin<br />
<br />
The Organism was also sensitized by some disinfectants used including Tea tree, PepperMint, Lysol 10% and Bleach 100%.<br />
<br />
==Genome Structure==<br />
<br />
Unknown due to complications from sequence reactions.<br />
<br />
==Cell Structure, Metabolism and Life Cycle==<br />
<br />
'''Gram Stain''' NS Mystery Organism shows to be Gram-positive under Gram stain reaction test with prominent purple color from Crystal Violet. <br />
<br />
'''Capsule Stain''' Under Capsule Stain test, the result is negative without any appearance of Capsule. <br />
<br />
'''Endospore test''' The Organism also shows Negative Endospore test result under Endospore Stain test. <br />
<br />
'''Motility test''' However, under the Motility test, there was a movement away from the stab line into the medium which indicates a Positive Motility test result. Non-motile organisms which lack flagella, are usually going to form a single line of growth that does not spread into the surrounding area. While a motile bacterium will grow and make a hazy zone around the stab line. <br />
<br />
'''Oxidase test''' Oxidase test has shown Positive result indicating Aerobic respiration.<br />
<br />
'''Triple Sugar Iron Agar''' NS Mystery Organism turns yellow/yellow under Triple Sugar Iron Agar (TSI) test points out fermentation of Lactose and Glucose.<br />
<br />
'''DNA Hydrolysis''' The Organism produces DNase enzyme under DNA hydrolysis test with a clearing around growth in the agar plate. <br />
<br />
'''Lipid Hydrolysis''' It also shows to have Lipase enzyme with a weak positive test result of Lipid Hydrolysis.<br />
<br />
==Physiology and Pathogenesis==<br />
<br />
'''Mannitol Salt Agar''' The Mannitol Salt Agar result for the organism indicated a positive result. The medium shows good yellow growth and is presumed Staphylococci because it is not inhibited by NaCl. The organism produces acid from Mannitol fermentation and is presumed possible pathogenic Staphylococcus aureus. <br />
<br />
'''Gram Stain''' A gram reaction test was done and indicated that the organism was gram positive.<br />
<br />
'''Lipid Hydrolysis''' Lipid hydrolysis was used to detect lipolytic bacteria, especially in high-fat dairy products. Lipase allows the organisms that produce it to break down lipids into smaller fragments. The test showed a weak positive.<br />
<br />
'''Starch Hydrolysis''' Results : Inconclusive/Unknown<br />
<br />
'''Phenol Red Broth''': Results : Negative Fermentation.<br />
<br />
'''Casein Hydrolysis''': Results : Negative. There was no clearing around the organism which indicates a negative test result. <br />
<br />
'''Gelatin Hydrolysis''': Results : Negative. The organism shows inability to produces Gelatinase. <br />
<br />
'''Oxidase Test''' The NS Mystery Organism is aerobic, and can use oxygen as a terminal electron acceptor in respiration. This was proven through a positive oxidase test. <br />
<br />
'''Eosin methylene blue agar''' EMB test is used to encourage the growth of fecal coliforms, to test drinking water. The result is based on lactose fermentation. The results were positive since pink colonies formed, indicating slow and weak fermentation.<br />
<br />
'''Phenylethyl Alcohol Agar''' The organism is not inhibited by phenylethyl alcohol as proven with Phenylethyl Alcohol Agar.<br />
<br />
==References==<br />
[Sample reference] [http://ijs.sgmjournals.org/cgi/reprint/50/2/489 Takai, K., Sugai, A., Itoh, T., and Horikoshi, K. "''Palaeococcus ferrophilus'' gen. nov., sp. nov., a barophilic, hyperthermophilic archaeon from a deep-sea hydrothermal vent chimney". ''International Journal of Systematic and Evolutionary Microbiology''. 2000. Volume 50. p. 489-500.]<br />
<br />
==Author==<br />
Page authored by Suchanart Apisaksiri and Sally Phonthaasa, students of Prof. Kristine Hollingsworth at Austin Community College.<br />
<br />
<!-- Do not remove this line-->[[Category:Pages edited by students of Kristine Hollingsworth at Austin Community College]]</div>Suchanart.apisaksirihttps://microbewiki.kenyon.edu/index.php?title=NS_Mystery_Organism&diff=132320NS Mystery Organism2017-12-08T20:32:31Z<p>Suchanart.apisaksiri: /* Physiology and Pathogenesis */</p>
<hr />
<div>{{Uncurated}}<br />
==Classification==<br />
<br />
Domain; Phylum; Class; Order; family [Others may be used. Use [http://www.ncbi.nlm.nih.gov/Taxonomy/ NCBI] link to find]<br />
<br />
Unknown <br />
<br />
===Species===<br />
<br />
{|<br />
| height="10" bgcolor="#FFDF95" |<br />
'''NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Tree&id=2&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy]'''<br />
|}<br />
<br />
''NS Mystery Organism''<br />
<br />
==Habitat Information ==<br />
Describe the location and conditions under which the organism was isolated.<br />
<br />
Date: 09/06/2017 <br />
<br />
Time: 18:49:00 CST<br />
<br />
Air Temperature: 83 ℉<br />
<br />
Humidity: 33%<br />
<br />
24 hr rainfall: 0 inches<br />
<br />
Pressure: 30.6 inch/ 1,017.0 mb<br />
<br />
Solar Radiation: 22.49 ms/m²<br />
<br />
Latitude: 30.144673 | Longitude: -97.841463<br />
<br />
The organism was isolated at a latitude of 30.144673 and a longitude of -97.841463 on September 6, 2017 at 18:49:00 CST. The conditions under which the NS Mystery Organism was isolated includes it being 83 degrees fahrenheit and 33% humidity. There were 0 inches of rainfall with a solar radiation of 22.49 ms/m^2.<br />
<br />
==Description and Significance==<br />
<br />
The colony morphology of NS Mystery Organism has a smooth surface with slightly raised elevation and an entire margin. The organism exhibits creamy yellow color. <br />
<br />
Cellular arrangement through microscopic observation is Diplobacilli. <br />
<br />
'''Significance'''<br />
<br />
The antimicrobial activity test has shown a zone of inhibitions with all the antibiotics used which are listed below.<br />
Antimicrobial agents used: <br />
1) Sulbactam,<br />
2. Ticracillin<br />
3. Cefoxitin<br />
4. Oxacillin <br />
5. Vancomycin<br />
<br />
The Organism was also sensitized by some disinfectants used including Tea tree, PepperMint, Lysol 10% and Bleach 100%.<br />
<br />
==Genome Structure==<br />
<br />
Unknown due to complications from sequence reactions.<br />
<br />
==Cell Structure, Metabolism and Life Cycle==<br />
<br />
'''Gram Stain''' NS Mystery Organism shows to be Gram-positive under Gram stain reaction test with prominent purple color from Crystal Violet. <br />
<br />
'''Capsule Stain''' Under Capsule Stain test, the result is negative without any appearance of Capsule. <br />
<br />
'''Endospore test''' The Organism also shows Negative Endospore test result under Endospore Stain test. <br />
<br />
'''Motility test''' However, under the Motility test, there was a movement away from the stab line into the medium which indicates a Positive Motility test result. Non-motile organisms which lack flagella, are usually going to form a single line of growth that does not spread into the surrounding area. While a motile bacterium will grow and make a hazy zone around the stab line. <br />
<br />
'''Oxidase test''' Oxidase test has shown Positive result indicating Aerobic respiration.<br />
<br />
'''Triple Sugar Iron Agar''' NS Mystery Organism turns yellow/yellow under Triple Sugar Iron Agar (TSI) test points out fermentation of Lactose and Glucose.<br />
<br />
'''DNA Hydrolysis''' The Organism produces DNase enzyme under DNA hydrolysis test with a clearing around growth in the agar plate. <br />
<br />
'''Lipid Hydrolysis''' It also shows to have Lipase enzyme with a weak positive test result of Lipid Hydrolysis.<br />
<br />
==Physiology and Pathogenesis==<br />
<br />
'''Mannitol Salt Agar''' The Mannitol Salt Agar result for the organism indicated a positive result. The medium shows good yellow growth and is presumed Staphylococci because it is not inhibited by NaCl. The organism produces acid from Mannitol fermentation and is presumed possible pathogenic Staphylococcus aureus. <br />
<br />
'''Gram Stain''' A gram reaction test was done and indicated that the organism was gram positive.<br />
<br />
'''Lipid Hydrolysis''' Lipid hydrolysis was used to detect lipolytic bacteria, especially in high-fat dairy products. Lipase allows the organisms that produce it to break down lipids into smaller fragments. The test showed a weak positive.<br />
<br />
'''Oxidase Test''' The NS Mystery Organism is aerobic, and can use oxygen as a terminal electron acceptor in respiration. This was proven through a positive oxidase test. <br />
<br />
'''Eosin methylene blue agar''' EMB test is used to encourage the growth of fecal coliforms, to test drinking water. The result is based on lactose fermentation. The results were positive since pink colonies formed, indicating slow and weak fermentation.<br />
<br />
'''Phenylethyl Alcohol Agar''' The organism is not inhibited by phenylethyl alcohol as proven with Phenylethyl Alcohol Agar.<br />
<br />
==References==<br />
[Sample reference] [http://ijs.sgmjournals.org/cgi/reprint/50/2/489 Takai, K., Sugai, A., Itoh, T., and Horikoshi, K. "''Palaeococcus ferrophilus'' gen. nov., sp. nov., a barophilic, hyperthermophilic archaeon from a deep-sea hydrothermal vent chimney". ''International Journal of Systematic and Evolutionary Microbiology''. 2000. Volume 50. p. 489-500.]<br />
<br />
==Author==<br />
Page authored by Suchanart Apisaksiri and Sally Phonthaasa, students of Prof. Kristine Hollingsworth at Austin Community College.<br />
<br />
<!-- Do not remove this line-->[[Category:Pages edited by students of Kristine Hollingsworth at Austin Community College]]</div>Suchanart.apisaksirihttps://microbewiki.kenyon.edu/index.php?title=NS_Mystery_Organism&diff=132318NS Mystery Organism2017-12-08T20:32:14Z<p>Suchanart.apisaksiri: /* Cell Structure, Metabolism and Life Cycle */</p>
<hr />
<div>{{Uncurated}}<br />
==Classification==<br />
<br />
Domain; Phylum; Class; Order; family [Others may be used. Use [http://www.ncbi.nlm.nih.gov/Taxonomy/ NCBI] link to find]<br />
<br />
Unknown <br />
<br />
===Species===<br />
<br />
{|<br />
| height="10" bgcolor="#FFDF95" |<br />
'''NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Tree&id=2&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy]'''<br />
|}<br />
<br />
''NS Mystery Organism''<br />
<br />
==Habitat Information ==<br />
Describe the location and conditions under which the organism was isolated.<br />
<br />
Date: 09/06/2017 <br />
<br />
Time: 18:49:00 CST<br />
<br />
Air Temperature: 83 ℉<br />
<br />
Humidity: 33%<br />
<br />
24 hr rainfall: 0 inches<br />
<br />
Pressure: 30.6 inch/ 1,017.0 mb<br />
<br />
Solar Radiation: 22.49 ms/m²<br />
<br />
Latitude: 30.144673 | Longitude: -97.841463<br />
<br />
The organism was isolated at a latitude of 30.144673 and a longitude of -97.841463 on September 6, 2017 at 18:49:00 CST. The conditions under which the NS Mystery Organism was isolated includes it being 83 degrees fahrenheit and 33% humidity. There were 0 inches of rainfall with a solar radiation of 22.49 ms/m^2.<br />
<br />
==Description and Significance==<br />
<br />
The colony morphology of NS Mystery Organism has a smooth surface with slightly raised elevation and an entire margin. The organism exhibits creamy yellow color. <br />
<br />
Cellular arrangement through microscopic observation is Diplobacilli. <br />
<br />
'''Significance'''<br />
<br />
The antimicrobial activity test has shown a zone of inhibitions with all the antibiotics used which are listed below.<br />
Antimicrobial agents used: <br />
1) Sulbactam,<br />
2. Ticracillin<br />
3. Cefoxitin<br />
4. Oxacillin <br />
5. Vancomycin<br />
<br />
The Organism was also sensitized by some disinfectants used including Tea tree, PepperMint, Lysol 10% and Bleach 100%.<br />
<br />
==Genome Structure==<br />
<br />
Unknown due to complications from sequence reactions.<br />
<br />
==Cell Structure, Metabolism and Life Cycle==<br />
<br />
'''Gram Stain''' NS Mystery Organism shows to be Gram-positive under Gram stain reaction test with prominent purple color from Crystal Violet. <br />
<br />
'''Capsule Stain''' Under Capsule Stain test, the result is negative without any appearance of Capsule. <br />
<br />
'''Endospore test''' The Organism also shows Negative Endospore test result under Endospore Stain test. <br />
<br />
'''Motility test''' However, under the Motility test, there was a movement away from the stab line into the medium which indicates a Positive Motility test result. Non-motile organisms which lack flagella, are usually going to form a single line of growth that does not spread into the surrounding area. While a motile bacterium will grow and make a hazy zone around the stab line. <br />
<br />
'''Oxidase test''' Oxidase test has shown Positive result indicating Aerobic respiration.<br />
<br />
'''Triple Sugar Iron Agar''' NS Mystery Organism turns yellow/yellow under Triple Sugar Iron Agar (TSI) test points out fermentation of Lactose and Glucose.<br />
<br />
'''DNA Hydrolysis''' The Organism produces DNase enzyme under DNA hydrolysis test with a clearing around growth in the agar plate. <br />
<br />
'''Lipid Hydrolysis''' It also shows to have Lipase enzyme with a weak positive test result of Lipid Hydrolysis.<br />
<br />
==Physiology and Pathogenesis==<br />
<br />
'''Mannitol Salt Agar''' The Mannitol Salt Agar result for the organism indicated a positive result. The medium shows good yellow growth and is presumed Staphylococci because it is not inhibited by NaCl. The organism produces acid from Mannitol fermentation and is presumed possible pathogenic Staphylococcus aureus. <br />
<br />
'''Gram Stain''' A gram reaction test was done and indicated that the organism was gram positive.<br />
<br />
In addition, a positive motility test and a positive DNA hydrolysis was tested. <br />
<br />
'''Lipid Hydrolysis''' Lipid hydrolysis was used to detect lipolytic bacteria, especially in high-fat dairy products. Lipase allows the organisms that produce it to break down lipids into smaller fragments. The test showed a weak positive.<br />
<br />
'''Oxidase Test''' The NS Mystery Organism is aerobic, and can use oxygen as a terminal electron acceptor in respiration. This was proven through a positive oxidase test. <br />
<br />
'''Eosin methylene blue agar''' EMB test is used to encourage the growth of fecal coliforms, to test drinking water. The result is based on lactose fermentation. The results were positive since pink colonies formed, indicating slow and weak fermentation.<br />
<br />
'''Phenylethyl Alcohol Agar''' The organism is not inhibited by phenylethyl alcohol as proven with Phenylethyl Alcohol Agar.<br />
<br />
==References==<br />
[Sample reference] [http://ijs.sgmjournals.org/cgi/reprint/50/2/489 Takai, K., Sugai, A., Itoh, T., and Horikoshi, K. "''Palaeococcus ferrophilus'' gen. nov., sp. nov., a barophilic, hyperthermophilic archaeon from a deep-sea hydrothermal vent chimney". ''International Journal of Systematic and Evolutionary Microbiology''. 2000. Volume 50. p. 489-500.]<br />
<br />
==Author==<br />
Page authored by Suchanart Apisaksiri and Sally Phonthaasa, students of Prof. Kristine Hollingsworth at Austin Community College.<br />
<br />
<!-- Do not remove this line-->[[Category:Pages edited by students of Kristine Hollingsworth at Austin Community College]]</div>Suchanart.apisaksirihttps://microbewiki.kenyon.edu/index.php?title=NS_Mystery_Organism&diff=132317NS Mystery Organism2017-12-08T20:31:21Z<p>Suchanart.apisaksiri: /* Physiology and Pathogenesis */</p>
<hr />
<div>{{Uncurated}}<br />
==Classification==<br />
<br />
Domain; Phylum; Class; Order; family [Others may be used. Use [http://www.ncbi.nlm.nih.gov/Taxonomy/ NCBI] link to find]<br />
<br />
Unknown <br />
<br />
===Species===<br />
<br />
{|<br />
| height="10" bgcolor="#FFDF95" |<br />
'''NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Tree&id=2&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy]'''<br />
|}<br />
<br />
''NS Mystery Organism''<br />
<br />
==Habitat Information ==<br />
Describe the location and conditions under which the organism was isolated.<br />
<br />
Date: 09/06/2017 <br />
<br />
Time: 18:49:00 CST<br />
<br />
Air Temperature: 83 ℉<br />
<br />
Humidity: 33%<br />
<br />
24 hr rainfall: 0 inches<br />
<br />
Pressure: 30.6 inch/ 1,017.0 mb<br />
<br />
Solar Radiation: 22.49 ms/m²<br />
<br />
Latitude: 30.144673 | Longitude: -97.841463<br />
<br />
The organism was isolated at a latitude of 30.144673 and a longitude of -97.841463 on September 6, 2017 at 18:49:00 CST. The conditions under which the NS Mystery Organism was isolated includes it being 83 degrees fahrenheit and 33% humidity. There were 0 inches of rainfall with a solar radiation of 22.49 ms/m^2.<br />
<br />
==Description and Significance==<br />
<br />
The colony morphology of NS Mystery Organism has a smooth surface with slightly raised elevation and an entire margin. The organism exhibits creamy yellow color. <br />
<br />
Cellular arrangement through microscopic observation is Diplobacilli. <br />
<br />
'''Significance'''<br />
<br />
The antimicrobial activity test has shown a zone of inhibitions with all the antibiotics used which are listed below.<br />
Antimicrobial agents used: <br />
1) Sulbactam,<br />
2. Ticracillin<br />
3. Cefoxitin<br />
4. Oxacillin <br />
5. Vancomycin<br />
<br />
The Organism was also sensitized by some disinfectants used including Tea tree, PepperMint, Lysol 10% and Bleach 100%.<br />
<br />
==Genome Structure==<br />
<br />
Unknown due to complications from sequence reactions.<br />
<br />
==Cell Structure, Metabolism and Life Cycle==<br />
<br />
'''Gram Stain''' NS Mystery Organism shows to be Gram-positive under Gram stain reaction test with prominent purple color from Crystal Violet. <br />
<br />
'''Capsule Stain''' Under Capsule Stain test, the result is negative without any appearance of Capsule. <br />
<br />
'''Endospore test''' The Organism also shows Negative Endospore test result under Endospore Stain test. <br />
<br />
'''Motility test''' However, under the Motility test, there was a movement away from the stab line into the medium which indicates a Positive Motility test result. <br />
<br />
'''Oxidase test''' Oxidase test has shown Positive result indicating Aerobic respiration.<br />
<br />
'''Triple Sugar Iron Agar''' NS Mystery Organism turns yellow/yellow under Triple Sugar Iron Agar (TSI) test points out fermentation of Lactose and Glucose.<br />
<br />
'''DNA Hydrolysis''' The Organism produces DNase enzyme under DNA hydrolysis test with a clearing around growth in the agar plate. <br />
<br />
'''Lipid Hydrolysis''' It also shows to have Lipase enzyme with a weak positive test result of Lipid Hydrolysis.<br />
<br />
==Physiology and Pathogenesis==<br />
<br />
'''Mannitol Salt Agar''' The Mannitol Salt Agar result for the organism indicated a positive result. The medium shows good yellow growth and is presumed Staphylococci because it is not inhibited by NaCl. The organism produces acid from Mannitol fermentation and is presumed possible pathogenic Staphylococcus aureus. <br />
<br />
'''Gram Stain''' A gram reaction test was done and indicated that the organism was gram positive.<br />
<br />
In addition, a positive motility test and a positive DNA hydrolysis was tested. <br />
<br />
'''Lipid Hydrolysis''' Lipid hydrolysis was used to detect lipolytic bacteria, especially in high-fat dairy products. Lipase allows the organisms that produce it to break down lipids into smaller fragments. The test showed a weak positive.<br />
<br />
'''Oxidase Test''' The NS Mystery Organism is aerobic, and can use oxygen as a terminal electron acceptor in respiration. This was proven through a positive oxidase test. <br />
<br />
'''Eosin methylene blue agar''' EMB test is used to encourage the growth of fecal coliforms, to test drinking water. The result is based on lactose fermentation. The results were positive since pink colonies formed, indicating slow and weak fermentation.<br />
<br />
'''Phenylethyl Alcohol Agar''' The organism is not inhibited by phenylethyl alcohol as proven with Phenylethyl Alcohol Agar.<br />
<br />
==References==<br />
[Sample reference] [http://ijs.sgmjournals.org/cgi/reprint/50/2/489 Takai, K., Sugai, A., Itoh, T., and Horikoshi, K. "''Palaeococcus ferrophilus'' gen. nov., sp. nov., a barophilic, hyperthermophilic archaeon from a deep-sea hydrothermal vent chimney". ''International Journal of Systematic and Evolutionary Microbiology''. 2000. Volume 50. p. 489-500.]<br />
<br />
==Author==<br />
Page authored by Suchanart Apisaksiri and Sally Phonthaasa, students of Prof. Kristine Hollingsworth at Austin Community College.<br />
<br />
<!-- Do not remove this line-->[[Category:Pages edited by students of Kristine Hollingsworth at Austin Community College]]</div>Suchanart.apisaksirihttps://microbewiki.kenyon.edu/index.php?title=NS_Mystery_Organism&diff=132316NS Mystery Organism2017-12-08T20:28:11Z<p>Suchanart.apisaksiri: /* Cell Structure, Metabolism and Life Cycle */</p>
<hr />
<div>{{Uncurated}}<br />
==Classification==<br />
<br />
Domain; Phylum; Class; Order; family [Others may be used. Use [http://www.ncbi.nlm.nih.gov/Taxonomy/ NCBI] link to find]<br />
<br />
Unknown <br />
<br />
===Species===<br />
<br />
{|<br />
| height="10" bgcolor="#FFDF95" |<br />
'''NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Tree&id=2&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy]'''<br />
|}<br />
<br />
''NS Mystery Organism''<br />
<br />
==Habitat Information ==<br />
Describe the location and conditions under which the organism was isolated.<br />
<br />
Date: 09/06/2017 <br />
<br />
Time: 18:49:00 CST<br />
<br />
Air Temperature: 83 ℉<br />
<br />
Humidity: 33%<br />
<br />
24 hr rainfall: 0 inches<br />
<br />
Pressure: 30.6 inch/ 1,017.0 mb<br />
<br />
Solar Radiation: 22.49 ms/m²<br />
<br />
Latitude: 30.144673 | Longitude: -97.841463<br />
<br />
The organism was isolated at a latitude of 30.144673 and a longitude of -97.841463 on September 6, 2017 at 18:49:00 CST. The conditions under which the NS Mystery Organism was isolated includes it being 83 degrees fahrenheit and 33% humidity. There were 0 inches of rainfall with a solar radiation of 22.49 ms/m^2.<br />
<br />
==Description and Significance==<br />
<br />
The colony morphology of NS Mystery Organism has a smooth surface with slightly raised elevation and an entire margin. The organism exhibits creamy yellow color. <br />
<br />
Cellular arrangement through microscopic observation is Diplobacilli. <br />
<br />
'''Significance'''<br />
<br />
The antimicrobial activity test has shown a zone of inhibitions with all the antibiotics used which are listed below.<br />
Antimicrobial agents used: <br />
1) Sulbactam,<br />
2. Ticracillin<br />
3. Cefoxitin<br />
4. Oxacillin <br />
5. Vancomycin<br />
<br />
The Organism was also sensitized by some disinfectants used including Tea tree, PepperMint, Lysol 10% and Bleach 100%.<br />
<br />
==Genome Structure==<br />
<br />
Unknown due to complications from sequence reactions.<br />
<br />
==Cell Structure, Metabolism and Life Cycle==<br />
<br />
'''Gram Stain''' NS Mystery Organism shows to be Gram-positive under Gram stain reaction test with prominent purple color from Crystal Violet. <br />
<br />
'''Capsule Stain''' Under Capsule Stain test, the result is negative without any appearance of Capsule. <br />
<br />
'''Endospore test''' The Organism also shows Negative Endospore test result under Endospore Stain test. <br />
<br />
'''Motility test''' However, under the Motility test, there was a movement away from the stab line into the medium which indicates a Positive Motility test result. <br />
<br />
'''Oxidase test''' Oxidase test has shown Positive result indicating Aerobic respiration.<br />
<br />
'''Triple Sugar Iron Agar''' NS Mystery Organism turns yellow/yellow under Triple Sugar Iron Agar (TSI) test points out fermentation of Lactose and Glucose.<br />
<br />
'''DNA Hydrolysis''' The Organism produces DNase enzyme under DNA hydrolysis test with a clearing around growth in the agar plate. <br />
<br />
'''Lipid Hydrolysis''' It also shows to have Lipase enzyme with a weak positive test result of Lipid Hydrolysis.<br />
<br />
==Physiology and Pathogenesis==<br />
<br />
The Mannitol Salt Agar result for the organism indicated a positive result. The medium shows good yellow growth and is presumed Staphylococci because it is not inhibited by NaCl. The organism produces acid from Mannitol fermentation and is presumed possible pathogenic Staphylococcus aureus. <br />
<br />
A gram reaction test was done and indicated that the organism was gram positive.<br />
<br />
In addition, a positive motility test and a positive DNA hydrolysis was tested. Non-motile organisms which lack flagella, are usually going to form a single line of growth that does not spread into the surrounding area. While a motile bacterium will grow and make a hazy zone around the stab line. <br />
<br />
Lipid hydrolysis was used to detect lipolytic bacteria, especially in high-fat dairy products. Lipase allows the organisms that produce it to break down lipids into smaller fragments. The test showed a weak positive.<br />
<br />
The NS Mystery Organism is aerobic, and can use oxygen as a terminal electron acceptor in respiration. This was proven through a positive oxidase test. <br />
<br />
EMB test is used to encourage the growth of fecal coliforms, to test drinking water. The result is based on lactose fermentation. The results were positive since pink colonies formed, indicating slow and weak fermentation.<br />
<br />
The organism is not inhibited by phenylethyl alcohol as proven with Phenylethyl Alcohol Agar.<br />
<br />
==References==<br />
[Sample reference] [http://ijs.sgmjournals.org/cgi/reprint/50/2/489 Takai, K., Sugai, A., Itoh, T., and Horikoshi, K. "''Palaeococcus ferrophilus'' gen. nov., sp. nov., a barophilic, hyperthermophilic archaeon from a deep-sea hydrothermal vent chimney". ''International Journal of Systematic and Evolutionary Microbiology''. 2000. Volume 50. p. 489-500.]<br />
<br />
==Author==<br />
Page authored by Suchanart Apisaksiri and Sally Phonthaasa, students of Prof. Kristine Hollingsworth at Austin Community College.<br />
<br />
<!-- Do not remove this line-->[[Category:Pages edited by students of Kristine Hollingsworth at Austin Community College]]</div>Suchanart.apisaksirihttps://microbewiki.kenyon.edu/index.php?title=NS_Mystery_Organism&diff=132315NS Mystery Organism2017-12-08T20:22:32Z<p>Suchanart.apisaksiri: /* Description and Significance */</p>
<hr />
<div>{{Uncurated}}<br />
==Classification==<br />
<br />
Domain; Phylum; Class; Order; family [Others may be used. Use [http://www.ncbi.nlm.nih.gov/Taxonomy/ NCBI] link to find]<br />
<br />
Unknown <br />
<br />
===Species===<br />
<br />
{|<br />
| height="10" bgcolor="#FFDF95" |<br />
'''NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Tree&id=2&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy]'''<br />
|}<br />
<br />
''NS Mystery Organism''<br />
<br />
==Habitat Information ==<br />
Describe the location and conditions under which the organism was isolated.<br />
<br />
Date: 09/06/2017 <br />
<br />
Time: 18:49:00 CST<br />
<br />
Air Temperature: 83 ℉<br />
<br />
Humidity: 33%<br />
<br />
24 hr rainfall: 0 inches<br />
<br />
Pressure: 30.6 inch/ 1,017.0 mb<br />
<br />
Solar Radiation: 22.49 ms/m²<br />
<br />
Latitude: 30.144673 | Longitude: -97.841463<br />
<br />
The organism was isolated at a latitude of 30.144673 and a longitude of -97.841463 on September 6, 2017 at 18:49:00 CST. The conditions under which the NS Mystery Organism was isolated includes it being 83 degrees fahrenheit and 33% humidity. There were 0 inches of rainfall with a solar radiation of 22.49 ms/m^2.<br />
<br />
==Description and Significance==<br />
<br />
The colony morphology of NS Mystery Organism has a smooth surface with slightly raised elevation and an entire margin. The organism exhibits creamy yellow color. <br />
<br />
Cellular arrangement through microscopic observation is Diplobacilli. <br />
<br />
'''Significance'''<br />
<br />
The antimicrobial activity test has shown a zone of inhibitions with all the antibiotics used which are listed below.<br />
Antimicrobial agents used: <br />
1) Sulbactam,<br />
2. Ticracillin<br />
3. Cefoxitin<br />
4. Oxacillin <br />
5. Vancomycin<br />
<br />
The Organism was also sensitized by some disinfectants used including Tea tree, PepperMint, Lysol 10% and Bleach 100%.<br />
<br />
==Genome Structure==<br />
<br />
Unknown due to complications from sequence reactions.<br />
<br />
==Cell Structure, Metabolism and Life Cycle==<br />
<br />
NS Mystery Organism shows to be Gram-positive under Gram stain reaction test with prominent purple color from Crystal Violet. <br />
<br />
Under Capsule Stain test, the result is negative without any appearance of Capsule. <br />
<br />
The Organism also shows Negative Endospore test result under Endospore Stain test. <br />
<br />
However, under the Motility test, there was a movement away from the stab line into the medium which indicates a Positive Motility test result. <br />
<br />
Oxidase test has shown Positive result indicating Aerobic respiration.<br />
<br />
NS Mystery Organism turns yellow/yellow under Triple Sugar Iron Agar (TSI) test points out fermentation of Lactose and Glucose.<br />
<br />
The Organism produces DNase enzyme under DNA hydrolysis test with a clearing around growth in the agar plate. <br />
<br />
It also shows to have Lipase enzyme with a weak positive test result of Lipid Hydrolysis.<br />
<br />
==Physiology and Pathogenesis==<br />
<br />
The Mannitol Salt Agar result for the organism indicated a positive result. The medium shows good yellow growth and is presumed Staphylococci because it is not inhibited by NaCl. The organism produces acid from Mannitol fermentation and is presumed possible pathogenic Staphylococcus aureus. <br />
<br />
A gram reaction test was done and indicated that the organism was gram positive.<br />
<br />
In addition, a positive motility test and a positive DNA hydrolysis was tested. Non-motile organisms which lack flagella, are usually going to form a single line of growth that does not spread into the surrounding area. While a motile bacterium will grow and make a hazy zone around the stab line. <br />
<br />
Lipid hydrolysis was used to detect lipolytic bacteria, especially in high-fat dairy products. Lipase allows the organisms that produce it to break down lipids into smaller fragments. The test showed a weak positive.<br />
<br />
The NS Mystery Organism is aerobic, and can use oxygen as a terminal electron acceptor in respiration. This was proven through a positive oxidase test. <br />
<br />
EMB test is used to encourage the growth of fecal coliforms, to test drinking water. The result is based on lactose fermentation. The results were positive since pink colonies formed, indicating slow and weak fermentation.<br />
<br />
The organism is not inhibited by phenylethyl alcohol as proven with Phenylethyl Alcohol Agar.<br />
<br />
==References==<br />
[Sample reference] [http://ijs.sgmjournals.org/cgi/reprint/50/2/489 Takai, K., Sugai, A., Itoh, T., and Horikoshi, K. "''Palaeococcus ferrophilus'' gen. nov., sp. nov., a barophilic, hyperthermophilic archaeon from a deep-sea hydrothermal vent chimney". ''International Journal of Systematic and Evolutionary Microbiology''. 2000. Volume 50. p. 489-500.]<br />
<br />
==Author==<br />
Page authored by Suchanart Apisaksiri and Sally Phonthaasa, students of Prof. Kristine Hollingsworth at Austin Community College.<br />
<br />
<!-- Do not remove this line-->[[Category:Pages edited by students of Kristine Hollingsworth at Austin Community College]]</div>Suchanart.apisaksirihttps://microbewiki.kenyon.edu/index.php?title=NS_Mystery_Organism&diff=132314NS Mystery Organism2017-12-08T20:22:12Z<p>Suchanart.apisaksiri: /* Description and Significance */</p>
<hr />
<div>{{Uncurated}}<br />
==Classification==<br />
<br />
Domain; Phylum; Class; Order; family [Others may be used. Use [http://www.ncbi.nlm.nih.gov/Taxonomy/ NCBI] link to find]<br />
<br />
Unknown <br />
<br />
===Species===<br />
<br />
{|<br />
| height="10" bgcolor="#FFDF95" |<br />
'''NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Tree&id=2&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy]'''<br />
|}<br />
<br />
''NS Mystery Organism''<br />
<br />
==Habitat Information ==<br />
Describe the location and conditions under which the organism was isolated.<br />
<br />
Date: 09/06/2017 <br />
<br />
Time: 18:49:00 CST<br />
<br />
Air Temperature: 83 ℉<br />
<br />
Humidity: 33%<br />
<br />
24 hr rainfall: 0 inches<br />
<br />
Pressure: 30.6 inch/ 1,017.0 mb<br />
<br />
Solar Radiation: 22.49 ms/m²<br />
<br />
Latitude: 30.144673 | Longitude: -97.841463<br />
<br />
The organism was isolated at a latitude of 30.144673 and a longitude of -97.841463 on September 6, 2017 at 18:49:00 CST. The conditions under which the NS Mystery Organism was isolated includes it being 83 degrees fahrenheit and 33% humidity. There were 0 inches of rainfall with a solar radiation of 22.49 ms/m^2.<br />
<br />
==Description and Significance==<br />
<br />
The colony morphology of NS Mystery Organism has a smooth surface with slightly raised elevation and an entire margin. The organism exhibits creamy yellow color. <br />
<br />
Cellular arrangement through microscopic observation is Diplobacilli. <br />
<br />
'''Significance'''<br />
The antimicrobial activity test has shown a zone of inhibitions with all the antibiotics used which are listed below.<br />
Antimicrobial agents used: <br />
1) Sulbactam,<br />
2. Ticracillin<br />
3. Cefoxitin<br />
4. Oxacillin <br />
5. Vancomycin<br />
<br />
The Organism was also sensitized by some disinfectants used including Tea tree, PepperMint, Lysol 10% and Bleach 100%.<br />
<br />
==Genome Structure==<br />
<br />
Unknown due to complications from sequence reactions.<br />
<br />
==Cell Structure, Metabolism and Life Cycle==<br />
<br />
NS Mystery Organism shows to be Gram-positive under Gram stain reaction test with prominent purple color from Crystal Violet. <br />
<br />
Under Capsule Stain test, the result is negative without any appearance of Capsule. <br />
<br />
The Organism also shows Negative Endospore test result under Endospore Stain test. <br />
<br />
However, under the Motility test, there was a movement away from the stab line into the medium which indicates a Positive Motility test result. <br />
<br />
Oxidase test has shown Positive result indicating Aerobic respiration.<br />
<br />
NS Mystery Organism turns yellow/yellow under Triple Sugar Iron Agar (TSI) test points out fermentation of Lactose and Glucose.<br />
<br />
The Organism produces DNase enzyme under DNA hydrolysis test with a clearing around growth in the agar plate. <br />
<br />
It also shows to have Lipase enzyme with a weak positive test result of Lipid Hydrolysis.<br />
<br />
==Physiology and Pathogenesis==<br />
<br />
The Mannitol Salt Agar result for the organism indicated a positive result. The medium shows good yellow growth and is presumed Staphylococci because it is not inhibited by NaCl. The organism produces acid from Mannitol fermentation and is presumed possible pathogenic Staphylococcus aureus. <br />
<br />
A gram reaction test was done and indicated that the organism was gram positive.<br />
<br />
In addition, a positive motility test and a positive DNA hydrolysis was tested. Non-motile organisms which lack flagella, are usually going to form a single line of growth that does not spread into the surrounding area. While a motile bacterium will grow and make a hazy zone around the stab line. <br />
<br />
Lipid hydrolysis was used to detect lipolytic bacteria, especially in high-fat dairy products. Lipase allows the organisms that produce it to break down lipids into smaller fragments. The test showed a weak positive.<br />
<br />
The NS Mystery Organism is aerobic, and can use oxygen as a terminal electron acceptor in respiration. This was proven through a positive oxidase test. <br />
<br />
EMB test is used to encourage the growth of fecal coliforms, to test drinking water. The result is based on lactose fermentation. The results were positive since pink colonies formed, indicating slow and weak fermentation.<br />
<br />
The organism is not inhibited by phenylethyl alcohol as proven with Phenylethyl Alcohol Agar.<br />
<br />
==References==<br />
[Sample reference] [http://ijs.sgmjournals.org/cgi/reprint/50/2/489 Takai, K., Sugai, A., Itoh, T., and Horikoshi, K. "''Palaeococcus ferrophilus'' gen. nov., sp. nov., a barophilic, hyperthermophilic archaeon from a deep-sea hydrothermal vent chimney". ''International Journal of Systematic and Evolutionary Microbiology''. 2000. Volume 50. p. 489-500.]<br />
<br />
==Author==<br />
Page authored by Suchanart Apisaksiri and Sally Phonthaasa, students of Prof. Kristine Hollingsworth at Austin Community College.<br />
<br />
<!-- Do not remove this line-->[[Category:Pages edited by students of Kristine Hollingsworth at Austin Community College]]</div>Suchanart.apisaksirihttps://microbewiki.kenyon.edu/index.php?title=NS_Mystery_Organism&diff=132311NS Mystery Organism2017-12-08T20:08:29Z<p>Suchanart.apisaksiri: /* Description and Significance */</p>
<hr />
<div>{{Uncurated}}<br />
==Classification==<br />
<br />
Domain; Phylum; Class; Order; family [Others may be used. Use [http://www.ncbi.nlm.nih.gov/Taxonomy/ NCBI] link to find]<br />
<br />
Unknown <br />
<br />
===Species===<br />
<br />
{|<br />
| height="10" bgcolor="#FFDF95" |<br />
'''NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Tree&id=2&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy]'''<br />
|}<br />
<br />
''NS Mystery Organism''<br />
<br />
==Habitat Information ==<br />
Describe the location and conditions under which the organism was isolated.<br />
<br />
Date: 09/06/2017 <br />
<br />
Time: 18:49:00 CST<br />
<br />
Air Temperature: 83 ℉<br />
<br />
Humidity: 33%<br />
<br />
24 hr rainfall: 0 inches<br />
<br />
Pressure: 30.6 inch/ 1,017.0 mb<br />
<br />
Solar Radiation: 22.49 ms/m²<br />
<br />
Latitude: 30.144673 | Longitude: -97.841463<br />
<br />
The organism was isolated at a latitude of 30.144673 and a longitude of -97.841463 on September 6, 2017 at 18:49:00 CST. The conditions under which the NS Mystery Organism was isolated includes it being 83 degrees fahrenheit and 33% humidity. There were 0 inches of rainfall with a solar radiation of 22.49 ms/m^2.<br />
<br />
==Description and Significance==<br />
<br />
The colony morphology of NS Mystery Organism has a smooth surface with slightly raised elevation and an entire margin. The organism exhibits creamy yellow color. <br />
<br />
Cellular arrangement through microscopic observation is Diplobacilli. The antimicrobial activity test has shown a zone of inhibitions with all the antibiotics used which are listed below.<br />
Antimicrobial agents used: <br />
1) Sulbactam,<br />
2. Ticracillin<br />
3. Cefoxitin<br />
4. Oxacillin <br />
5. Vancomycin<br />
<br />
The Organism was also sensitized by some disinfectants used including Tea tree, PepperMint, Lysol 10% and Bleach 100%.<br />
<br />
==Genome Structure==<br />
<br />
Unknown due to complications from sequence reactions.<br />
<br />
==Cell Structure, Metabolism and Life Cycle==<br />
<br />
NS Mystery Organism shows to be Gram-positive under Gram stain reaction test with prominent purple color from Crystal Violet. <br />
<br />
Under Capsule Stain test, the result is negative without any appearance of Capsule. <br />
<br />
The Organism also shows Negative Endospore test result under Endospore Stain test. <br />
<br />
However, under the Motility test, there was a movement away from the stab line into the medium which indicates a Positive Motility test result. <br />
<br />
Oxidase test has shown Positive result indicating Aerobic respiration.<br />
<br />
NS Mystery Organism turns yellow/yellow under Triple Sugar Iron Agar (TSI) test points out fermentation of Lactose and Glucose.<br />
<br />
The Organism produces DNase enzyme under DNA hydrolysis test with a clearing around growth in the agar plate. <br />
<br />
It also shows to have Lipase enzyme with a weak positive test result of Lipid Hydrolysis.<br />
<br />
==Physiology and Pathogenesis==<br />
<br />
The Mannitol Salt Agar result for the organism indicated a positive result. The medium shows good yellow growth and is presumed Staphylococci because it is not inhibited by NaCl. The organism produces acid from Mannitol fermentation and is presumed possible pathogenic Staphylococcus aureus. <br />
<br />
A gram reaction test was done and indicated that the organism was gram positive.<br />
<br />
In addition, a positive motility test and a positive DNA hydrolysis was tested. Non-motile organisms which lack flagella, are usually going to form a single line of growth that does not spread into the surrounding area. While a motile bacterium will grow and make a hazy zone around the stab line. <br />
<br />
Lipid hydrolysis was used to detect lipolytic bacteria, especially in high-fat dairy products. Lipase allows the organisms that produce it to break down lipids into smaller fragments. The test showed a weak positive.<br />
<br />
The NS Mystery Organism is aerobic, and can use oxygen as a terminal electron acceptor in respiration. This was proven through a positive oxidase test. <br />
<br />
EMB test is used to encourage the growth of fecal coliforms, to test drinking water. The result is based on lactose fermentation. The results were positive since pink colonies formed, indicating slow and weak fermentation.<br />
<br />
The organism is not inhibited by phenylethyl alcohol as proven with Phenylethyl Alcohol Agar.<br />
<br />
==References==<br />
[Sample reference] [http://ijs.sgmjournals.org/cgi/reprint/50/2/489 Takai, K., Sugai, A., Itoh, T., and Horikoshi, K. "''Palaeococcus ferrophilus'' gen. nov., sp. nov., a barophilic, hyperthermophilic archaeon from a deep-sea hydrothermal vent chimney". ''International Journal of Systematic and Evolutionary Microbiology''. 2000. Volume 50. p. 489-500.]<br />
<br />
==Author==<br />
Page authored by Suchanart Apisaksiri and Sally Phonthaasa, students of Prof. Kristine Hollingsworth at Austin Community College.<br />
<br />
<!-- Do not remove this line-->[[Category:Pages edited by students of Kristine Hollingsworth at Austin Community College]]</div>Suchanart.apisaksirihttps://microbewiki.kenyon.edu/index.php?title=NS_Mystery_Organism&diff=132310NS Mystery Organism2017-12-08T19:56:09Z<p>Suchanart.apisaksiri: /* Physiology and Pathogenesis */</p>
<hr />
<div>{{Uncurated}}<br />
==Classification==<br />
<br />
Domain; Phylum; Class; Order; family [Others may be used. Use [http://www.ncbi.nlm.nih.gov/Taxonomy/ NCBI] link to find]<br />
<br />
Unknown <br />
<br />
===Species===<br />
<br />
{|<br />
| height="10" bgcolor="#FFDF95" |<br />
'''NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Tree&id=2&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy]'''<br />
|}<br />
<br />
''NS Mystery Organism''<br />
<br />
==Habitat Information ==<br />
Describe the location and conditions under which the organism was isolated.<br />
<br />
Date: 09/06/2017 <br />
<br />
Time: 18:49:00 CST<br />
<br />
Air Temperature: 83 ℉<br />
<br />
Humidity: 33%<br />
<br />
24 hr rainfall: 0 inches<br />
<br />
Pressure: 30.6 inch/ 1,017.0 mb<br />
<br />
Solar Radiation: 22.49 ms/m²<br />
<br />
Latitude: 30.144673 | Longitude: -97.841463<br />
<br />
The organism was isolated at a latitude of 30.144673 and a longitude of -97.841463 on September 6, 2017 at 18:49:00 CST. The conditions under which the NS Mystery Organism was isolated includes it being 83 degrees fahrenheit and 33% humidity. There were 0 inches of rainfall with a solar radiation of 22.49 ms/m^2.<br />
<br />
==Description and Significance==<br />
Describe the appearance (colonial and cellular), possible antimicrobial activity etc. of the organism, and why the organism might be significant.<br />
<br />
The colony morphology of NS Mystery Organism has a smooth surface with slightly raised elevation and an entire margin. The organism exhibits creamy yellow color. <br />
<br />
Cellular arrangement through microscopic observation is Diplobacilli. The antimicrobial activity test has shown a zone of inhibitions with all the antibiotics used. The Organism was also sensitized by some disinfectants used including Tea tree, PepperMint, Lysol 10% and Bleach 100%.<br />
<br />
==Genome Structure==<br />
<br />
Unknown due to complications from sequence reactions.<br />
<br />
==Cell Structure, Metabolism and Life Cycle==<br />
<br />
NS Mystery Organism shows to be Gram-positive under Gram stain reaction test with prominent purple color from Crystal Violet. <br />
<br />
Under Capsule Stain test, the result is negative without any appearance of Capsule. <br />
<br />
The Organism also shows Negative Endospore test result under Endospore Stain test. <br />
<br />
However, under the Motility test, there was a movement away from the stab line into the medium which indicates a Positive Motility test result. <br />
<br />
Oxidase test has shown Positive result indicating Aerobic respiration.<br />
<br />
NS Mystery Organism turns yellow/yellow under Triple Sugar Iron Agar (TSI) test points out fermentation of Lactose and Glucose.<br />
<br />
The Organism produces DNase enzyme under DNA hydrolysis test with a clearing around growth in the agar plate. <br />
<br />
It also shows to have Lipase enzyme with a weak positive test result of Lipid Hydrolysis.<br />
<br />
==Physiology and Pathogenesis==<br />
<br />
The Mannitol Salt Agar result for the organism indicated a positive result. The medium shows good yellow growth and is presumed Staphylococci because it is not inhibited by NaCl. The organism produces acid from Mannitol fermentation and is presumed possible pathogenic Staphylococcus aureus. <br />
<br />
A gram reaction test was done and indicated that the organism was gram positive.<br />
<br />
In addition, a positive motility test and a positive DNA hydrolysis was tested. Non-motile organisms which lack flagella, are usually going to form a single line of growth that does not spread into the surrounding area. While a motile bacterium will grow and make a hazy zone around the stab line. <br />
<br />
Lipid hydrolysis was used to detect lipolytic bacteria, especially in high-fat dairy products. Lipase allows the organisms that produce it to break down lipids into smaller fragments. The test showed a weak positive.<br />
<br />
The NS Mystery Organism is aerobic, and can use oxygen as a terminal electron acceptor in respiration. This was proven through a positive oxidase test. <br />
<br />
EMB test is used to encourage the growth of fecal coliforms, to test drinking water. The result is based on lactose fermentation. The results were positive since pink colonies formed, indicating slow and weak fermentation.<br />
<br />
The organism is not inhibited by phenylethyl alcohol as proven with Phenylethyl Alcohol Agar.<br />
<br />
==References==<br />
[Sample reference] [http://ijs.sgmjournals.org/cgi/reprint/50/2/489 Takai, K., Sugai, A., Itoh, T., and Horikoshi, K. "''Palaeococcus ferrophilus'' gen. nov., sp. nov., a barophilic, hyperthermophilic archaeon from a deep-sea hydrothermal vent chimney". ''International Journal of Systematic and Evolutionary Microbiology''. 2000. Volume 50. p. 489-500.]<br />
<br />
==Author==<br />
Page authored by Suchanart Apisaksiri and Sally Phonthaasa, students of Prof. Kristine Hollingsworth at Austin Community College.<br />
<br />
<!-- Do not remove this line-->[[Category:Pages edited by students of Kristine Hollingsworth at Austin Community College]]</div>Suchanart.apisaksirihttps://microbewiki.kenyon.edu/index.php?title=NS_Mystery_Organism&diff=132308NS Mystery Organism2017-12-08T19:53:41Z<p>Suchanart.apisaksiri: /* Cell Structure, Metabolism and Life Cycle */</p>
<hr />
<div>{{Uncurated}}<br />
==Classification==<br />
<br />
Domain; Phylum; Class; Order; family [Others may be used. Use [http://www.ncbi.nlm.nih.gov/Taxonomy/ NCBI] link to find]<br />
<br />
Unknown <br />
<br />
===Species===<br />
<br />
{|<br />
| height="10" bgcolor="#FFDF95" |<br />
'''NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Tree&id=2&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy]'''<br />
|}<br />
<br />
''NS Mystery Organism''<br />
<br />
==Habitat Information ==<br />
Describe the location and conditions under which the organism was isolated.<br />
<br />
Date: 09/06/2017 <br />
<br />
Time: 18:49:00 CST<br />
<br />
Air Temperature: 83 ℉<br />
<br />
Humidity: 33%<br />
<br />
24 hr rainfall: 0 inches<br />
<br />
Pressure: 30.6 inch/ 1,017.0 mb<br />
<br />
Solar Radiation: 22.49 ms/m²<br />
<br />
Latitude: 30.144673 | Longitude: -97.841463<br />
<br />
The organism was isolated at a latitude of 30.144673 and a longitude of -97.841463 on September 6, 2017 at 18:49:00 CST. The conditions under which the NS Mystery Organism was isolated includes it being 83 degrees fahrenheit and 33% humidity. There were 0 inches of rainfall with a solar radiation of 22.49 ms/m^2.<br />
<br />
==Description and Significance==<br />
Describe the appearance (colonial and cellular), possible antimicrobial activity etc. of the organism, and why the organism might be significant.<br />
<br />
The colony morphology of NS Mystery Organism has a smooth surface with slightly raised elevation and an entire margin. The organism exhibits creamy yellow color. <br />
<br />
Cellular arrangement through microscopic observation is Diplobacilli. The antimicrobial activity test has shown a zone of inhibitions with all the antibiotics used. The Organism was also sensitized by some disinfectants used including Tea tree, PepperMint, Lysol 10% and Bleach 100%.<br />
<br />
==Genome Structure==<br />
<br />
Unknown due to complications from sequence reactions.<br />
<br />
==Cell Structure, Metabolism and Life Cycle==<br />
<br />
NS Mystery Organism shows to be Gram-positive under Gram stain reaction test with prominent purple color from Crystal Violet. <br />
<br />
Under Capsule Stain test, the result is negative without any appearance of Capsule. <br />
<br />
The Organism also shows Negative Endospore test result under Endospore Stain test. <br />
<br />
However, under the Motility test, there was a movement away from the stab line into the medium which indicates a Positive Motility test result. <br />
<br />
Oxidase test has shown Positive result indicating Aerobic respiration.<br />
<br />
NS Mystery Organism turns yellow/yellow under Triple Sugar Iron Agar (TSI) test points out fermentation of Lactose and Glucose.<br />
<br />
The Organism produces DNase enzyme under DNA hydrolysis test with a clearing around growth in the agar plate. <br />
<br />
It also shows to have Lipase enzyme with a weak positive test result of Lipid Hydrolysis.<br />
<br />
==Physiology and Pathogenesis==<br />
<br />
The Mannitol Salt Agar result for the organism indicated a positive result. The medium shows good yellow growth and is presumed Staphylococci because it is not inhibited by NaCl. The organism can produces acid from Mannitol fermentation and is presumed possible pathogenic Staphylococcus aureus. <br />
A gram reaction test was done and indicated that the organism was gram positive. In addition, a positive motility test and a positive DNA hydrolysis was tested. Non-motile organisms which lack flagella, are usually going to form a single line of growth that does not spread into the surrounding area. While a motile bacterium will grow and make a hazy zone around the stab line. <br />
Lipid hydrolysis was used to detect lipolytic bacteria, especially in high-fat dairy products. Lipase allows the organisms that produce it to break down lipids into smaller fragments. The test showed a weak positive. The NS Mystery Organism is aerobic, and can use oxygen as a terminal electron acceptor in respiration. This was proven through a positive oxidase test. EMB test is used to encourage the growth of fecal coliforms, to test drinking water. The result is based on lactose fermentation. The results were positive since pink colonies formed, indicating slow and weak fermentation.The organism is not inhibited by phenylethyl alcohol as proven with Phenylethyl Alcohol Agar.<br />
<br />
==References==<br />
[Sample reference] [http://ijs.sgmjournals.org/cgi/reprint/50/2/489 Takai, K., Sugai, A., Itoh, T., and Horikoshi, K. "''Palaeococcus ferrophilus'' gen. nov., sp. nov., a barophilic, hyperthermophilic archaeon from a deep-sea hydrothermal vent chimney". ''International Journal of Systematic and Evolutionary Microbiology''. 2000. Volume 50. p. 489-500.]<br />
<br />
==Author==<br />
Page authored by Suchanart Apisaksiri and Sally Phonthaasa, students of Prof. Kristine Hollingsworth at Austin Community College.<br />
<br />
<!-- Do not remove this line-->[[Category:Pages edited by students of Kristine Hollingsworth at Austin Community College]]</div>Suchanart.apisaksirihttps://microbewiki.kenyon.edu/index.php?title=NS_Mystery_Organism&diff=132307NS Mystery Organism2017-12-08T19:49:20Z<p>Suchanart.apisaksiri: /* Physiology and Pathogenesis */</p>
<hr />
<div>{{Uncurated}}<br />
==Classification==<br />
<br />
Domain; Phylum; Class; Order; family [Others may be used. Use [http://www.ncbi.nlm.nih.gov/Taxonomy/ NCBI] link to find]<br />
<br />
Unknown <br />
<br />
===Species===<br />
<br />
{|<br />
| height="10" bgcolor="#FFDF95" |<br />
'''NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Tree&id=2&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy]'''<br />
|}<br />
<br />
''NS Mystery Organism''<br />
<br />
==Habitat Information ==<br />
Describe the location and conditions under which the organism was isolated.<br />
<br />
Date: 09/06/2017 <br />
<br />
Time: 18:49:00 CST<br />
<br />
Air Temperature: 83 ℉<br />
<br />
Humidity: 33%<br />
<br />
24 hr rainfall: 0 inches<br />
<br />
Pressure: 30.6 inch/ 1,017.0 mb<br />
<br />
Solar Radiation: 22.49 ms/m²<br />
<br />
Latitude: 30.144673 | Longitude: -97.841463<br />
<br />
The organism was isolated at a latitude of 30.144673 and a longitude of -97.841463 on September 6, 2017 at 18:49:00 CST. The conditions under which the NS Mystery Organism was isolated includes it being 83 degrees fahrenheit and 33% humidity. There were 0 inches of rainfall with a solar radiation of 22.49 ms/m^2.<br />
<br />
==Description and Significance==<br />
Describe the appearance (colonial and cellular), possible antimicrobial activity etc. of the organism, and why the organism might be significant.<br />
<br />
The colony morphology of NS Mystery Organism has a smooth surface with slightly raised elevation and an entire margin. The organism exhibits creamy yellow color. <br />
<br />
Cellular arrangement through microscopic observation is Diplobacilli. The antimicrobial activity test has shown a zone of inhibitions with all the antibiotics used. The Organism was also sensitized by some disinfectants used including Tea tree, PepperMint, Lysol 10% and Bleach 100%.<br />
<br />
==Genome Structure==<br />
<br />
Unknown due to complications from sequence reactions.<br />
<br />
==Cell Structure, Metabolism and Life Cycle==<br />
Interesting features of cell structure; how it gains energy; what important molecules it produces.<br />
<br />
<br />
NS Mystery Organism shows to be Gram-positive under Gram stain reaction test with prominent purple color from Crystal Violet. <br />
<br />
Under Capsule Stain test, the result is negative without any appearance of Capsule. The Organism also shows Negative Endospore test result under Endospore Stain test. However, under the Motility test, there was a movement away from the stab line into the medium which indicates a Positive Motility test result. <br />
<br />
Oxidase test has shown Positive result indicating Aerobic respiration. NS Mystery Organism turns yellow/yellow under Triple Sugar Iron Agar (TSI) test points out fermentation of Lactose and Glucose.<br />
<br />
The Organism produces DNase enzyme under DNA hydrolysis test with a clearing around growth in the agar plate. It also shows to have Lipase enzyme with a weak positive test result of Lipid Hydrolysis.<br />
<br />
==Physiology and Pathogenesis==<br />
<br />
The Mannitol Salt Agar result for the organism indicated a positive result. The medium shows good yellow growth and is presumed Staphylococci because it is not inhibited by NaCl. The organism can produces acid from Mannitol fermentation and is presumed possible pathogenic Staphylococcus aureus. <br />
A gram reaction test was done and indicated that the organism was gram positive. In addition, a positive motility test and a positive DNA hydrolysis was tested. Non-motile organisms which lack flagella, are usually going to form a single line of growth that does not spread into the surrounding area. While a motile bacterium will grow and make a hazy zone around the stab line. <br />
Lipid hydrolysis was used to detect lipolytic bacteria, especially in high-fat dairy products. Lipase allows the organisms that produce it to break down lipids into smaller fragments. The test showed a weak positive. The NS Mystery Organism is aerobic, and can use oxygen as a terminal electron acceptor in respiration. This was proven through a positive oxidase test. EMB test is used to encourage the growth of fecal coliforms, to test drinking water. The result is based on lactose fermentation. The results were positive since pink colonies formed, indicating slow and weak fermentation.The organism is not inhibited by phenylethyl alcohol as proven with Phenylethyl Alcohol Agar.<br />
<br />
==References==<br />
[Sample reference] [http://ijs.sgmjournals.org/cgi/reprint/50/2/489 Takai, K., Sugai, A., Itoh, T., and Horikoshi, K. "''Palaeococcus ferrophilus'' gen. nov., sp. nov., a barophilic, hyperthermophilic archaeon from a deep-sea hydrothermal vent chimney". ''International Journal of Systematic and Evolutionary Microbiology''. 2000. Volume 50. p. 489-500.]<br />
<br />
==Author==<br />
Page authored by Suchanart Apisaksiri and Sally Phonthaasa, students of Prof. Kristine Hollingsworth at Austin Community College.<br />
<br />
<!-- Do not remove this line-->[[Category:Pages edited by students of Kristine Hollingsworth at Austin Community College]]</div>Suchanart.apisaksirihttps://microbewiki.kenyon.edu/index.php?title=NS_Mystery_Organism&diff=131954NS Mystery Organism2017-12-06T05:16:12Z<p>Suchanart.apisaksiri: /* Physiology and Pathogenesis */</p>
<hr />
<div>{{Uncurated}}<br />
==Classification==<br />
<br />
Domain; Phylum; Class; Order; family [Others may be used. Use [http://www.ncbi.nlm.nih.gov/Taxonomy/ NCBI] link to find]<br />
<br />
Unknown <br />
<br />
===Species===<br />
<br />
{|<br />
| height="10" bgcolor="#FFDF95" |<br />
'''NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Tree&id=2&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy]'''<br />
|}<br />
<br />
''NS Mystery Organism''<br />
<br />
==Habitat Information ==<br />
Describe the location and conditions under which the organism was isolated.<br />
<br />
Date: 09/06/2017 <br />
<br />
Time: 18:49:00 CST<br />
<br />
Air Temperature: 83 ℉<br />
<br />
Humidity: 33%<br />
<br />
24 hr rainfall: 0 inches<br />
<br />
Pressure: 30.6 inch/ 1,017.0 mb<br />
<br />
Solar Radiation: 22.49 ms/m²<br />
<br />
Latitude: 30.144673 | Longitude: -97.841463<br />
<br />
The organism was isolated at a latitude of 30.144673 and a longitude of -97.841463 on September 6, 2017 at 18:49:00 CST. The conditions under which the NS Mystery Organism was isolated includes it being 83 degrees fahrenheit and 33% humidity. There were 0 inches of rainfall with a solar radiation of 22.49 ms/m^2.<br />
<br />
==Description and Significance==<br />
Describe the appearance (colonial and cellular), possible antimicrobial activity etc. of the organism, and why the organism might be significant.<br />
<br />
The colony morphology of NS Mystery Organism has a smooth surface with slightly raised elevation and an entire margin. The organism exhibits creamy yellow color. <br />
<br />
Cellular arrangement through microscopic observation is Diplobacilli. The antimicrobial activity test has shown a zone of inhibitions with all the antibiotics used. The Organism was also sensitized by some disinfectants used including Tea tree, PepperMint, Lysol 10% and Bleach 100%.<br />
<br />
==Genome Structure==<br />
<br />
Unknown due to complications from sequence reactions.<br />
<br />
==Cell Structure, Metabolism and Life Cycle==<br />
Interesting features of cell structure; how it gains energy; what important molecules it produces.<br />
<br />
<br />
NS Mystery Organism shows to be Gram-positive under Gram stain reaction test with prominent purple color from Crystal Violet. <br />
<br />
Under Capsule Stain test, the result is negative without any appearance of Capsule. The Organism also shows Negative Endospore test result under Endospore Stain test. However, under the Motility test, there was a movement away from the stab line into the medium which indicates a Positive Motility test result. <br />
<br />
Oxidase test has shown Positive result indicating Aerobic respiration. NS Mystery Organism turns yellow/yellow under Triple Sugar Iron Agar (TSI) test points out fermentation of Lactose and Glucose.<br />
<br />
The Organism produces DNase enzyme under DNA hydrolysis test with a clearing around growth in the agar plate. It also shows to have Lipase enzyme with a weak positive test result of Lipid Hydrolysis.<br />
<br />
==Physiology and Pathogenesis==<br />
Biochemical characteristics, enzymes made, other characteristics that may be used to identify the organism; contributions to environment (if any).<br><br />
If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.<br><br><br />
<br />
The Mannitol Salt Agar result for the organism indicated a positive result. The medium shows good yellow growth and is presumed Staphylococci because it is not inhibited by NaCl. The organism can produces acid from Mannitol fermentation and is presumed possible pathogenic Staphylococcus aureus. <br />
A gram reaction test was done and indicated that the organism was gram positive. In addition, a positive motility test and a positive DNA hydrolysis was tested. Non-motile organisms which lack flagella, are usually going to form a single line of growth that does not spread into the surrounding area. While a motile bacterium will grow and make a hazy zone around the stab line. <br />
Lipid hydrolysis was used to detect lipolytic bacteria, especially in high-fat dairy products. Lipase allows the organisms that produce it to break down lipids into smaller fragments. The test showed a weak positive. The NS Mystery Organism is aerobic, and can use oxygen as a terminal electron acceptor in respiration. This was proven through a positive oxidase test. EMB test is used to encourage the growth of fecal coliforms, to test drinking water. The result is based on lactose fermentation. The results were positive since pink colonies formed, indicating slow and weak fermentation.The organism is not inhibited by phenylethyl alcohol as proven with Phenylethyl Alcohol Agar.<br />
<br />
==References==<br />
[Sample reference] [http://ijs.sgmjournals.org/cgi/reprint/50/2/489 Takai, K., Sugai, A., Itoh, T., and Horikoshi, K. "''Palaeococcus ferrophilus'' gen. nov., sp. nov., a barophilic, hyperthermophilic archaeon from a deep-sea hydrothermal vent chimney". ''International Journal of Systematic and Evolutionary Microbiology''. 2000. Volume 50. p. 489-500.]<br />
<br />
==Author==<br />
Page authored by Suchanart Apisaksiri and Sally Phonthaasa, students of Prof. Kristine Hollingsworth at Austin Community College.<br />
<br />
<!-- Do not remove this line-->[[Category:Pages edited by students of Kristine Hollingsworth at Austin Community College]]</div>Suchanart.apisaksirihttps://microbewiki.kenyon.edu/index.php?title=NS_Mystery_Organism&diff=131953NS Mystery Organism2017-12-06T05:13:53Z<p>Suchanart.apisaksiri: /* Classification */</p>
<hr />
<div>{{Uncurated}}<br />
==Classification==<br />
<br />
Domain; Phylum; Class; Order; family [Others may be used. Use [http://www.ncbi.nlm.nih.gov/Taxonomy/ NCBI] link to find]<br />
<br />
Unknown <br />
<br />
===Species===<br />
<br />
{|<br />
| height="10" bgcolor="#FFDF95" |<br />
'''NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Tree&id=2&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy]'''<br />
|}<br />
<br />
''NS Mystery Organism''<br />
<br />
==Habitat Information ==<br />
Describe the location and conditions under which the organism was isolated.<br />
<br />
Date: 09/06/2017 <br />
<br />
Time: 18:49:00 CST<br />
<br />
Air Temperature: 83 ℉<br />
<br />
Humidity: 33%<br />
<br />
24 hr rainfall: 0 inches<br />
<br />
Pressure: 30.6 inch/ 1,017.0 mb<br />
<br />
Solar Radiation: 22.49 ms/m²<br />
<br />
Latitude: 30.144673 | Longitude: -97.841463<br />
<br />
The organism was isolated at a latitude of 30.144673 and a longitude of -97.841463 on September 6, 2017 at 18:49:00 CST. The conditions under which the NS Mystery Organism was isolated includes it being 83 degrees fahrenheit and 33% humidity. There were 0 inches of rainfall with a solar radiation of 22.49 ms/m^2.<br />
<br />
==Description and Significance==<br />
Describe the appearance (colonial and cellular), possible antimicrobial activity etc. of the organism, and why the organism might be significant.<br />
<br />
The colony morphology of NS Mystery Organism has a smooth surface with slightly raised elevation and an entire margin. The organism exhibits creamy yellow color. <br />
<br />
Cellular arrangement through microscopic observation is Diplobacilli. The antimicrobial activity test has shown a zone of inhibitions with all the antibiotics used. The Organism was also sensitized by some disinfectants used including Tea tree, PepperMint, Lysol 10% and Bleach 100%.<br />
<br />
==Genome Structure==<br />
<br />
Unknown due to complications from sequence reactions.<br />
<br />
==Cell Structure, Metabolism and Life Cycle==<br />
Interesting features of cell structure; how it gains energy; what important molecules it produces.<br />
<br />
<br />
NS Mystery Organism shows to be Gram-positive under Gram stain reaction test with prominent purple color from Crystal Violet. <br />
<br />
Under Capsule Stain test, the result is negative without any appearance of Capsule. The Organism also shows Negative Endospore test result under Endospore Stain test. However, under the Motility test, there was a movement away from the stab line into the medium which indicates a Positive Motility test result. <br />
<br />
Oxidase test has shown Positive result indicating Aerobic respiration. NS Mystery Organism turns yellow/yellow under Triple Sugar Iron Agar (TSI) test points out fermentation of Lactose and Glucose.<br />
<br />
The Organism produces DNase enzyme under DNA hydrolysis test with a clearing around growth in the agar plate. It also shows to have Lipase enzyme with a weak positive test result of Lipid Hydrolysis.<br />
<br />
==Physiology and Pathogenesis==<br />
Biochemical characteristics, enzymes made, other characteristics that may be used to identify the organism; contributions to environment (if any).<br><br />
If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.<br><br><br />
<br />
The Mannitol Salt Agar result for the organism indicated a positive result. The medium shows good yellow growth and is presumed Staphylococci because it is not inhibited by NaCl. The organism can produces acid from Mannitol fermentation and is presumed possible pathogenic Staphylococcus aureus. A gram reaction test was done and indicated that the organism was gram positive. In addition, a positive motility test and a positive DNA hydrolysis was tested. Non-motile organisms which lack flagella, are usually going to form a single line of growth that does not spread into the surrounding area. While a motile bacterium will grow and make a hazy zone around the stab line. Lipid hydrolysis was used to detect lipolytic bacteria, especially in high-fat dairy products. Lipase allows the organisms that produce it to break down lipids into smaller fragments. The test showed a weak positive. The NS Mystery Organism is aerobic, and can use oxygen as a terminal electron acceptor in respiration. This was proven through a positive oxidase test. EMB test is used to encourage the growth of fecal coliforms, to test drinking water. The result is based on lactose fermentation. The results were positive since pink colonies formed, indicating slow and weak fermentation.The organism is not inhibited by phenylethyl alcohol as proven with Phenylethyl Alcohol Agar.<br />
<br />
==References==<br />
[Sample reference] [http://ijs.sgmjournals.org/cgi/reprint/50/2/489 Takai, K., Sugai, A., Itoh, T., and Horikoshi, K. "''Palaeococcus ferrophilus'' gen. nov., sp. nov., a barophilic, hyperthermophilic archaeon from a deep-sea hydrothermal vent chimney". ''International Journal of Systematic and Evolutionary Microbiology''. 2000. Volume 50. p. 489-500.]<br />
<br />
==Author==<br />
Page authored by Suchanart Apisaksiri and Sally Phonthaasa, students of Prof. Kristine Hollingsworth at Austin Community College.<br />
<br />
<!-- Do not remove this line-->[[Category:Pages edited by students of Kristine Hollingsworth at Austin Community College]]</div>Suchanart.apisaksirihttps://microbewiki.kenyon.edu/index.php?title=NS_Mystery_Organism&diff=131952NS Mystery Organism2017-12-06T05:13:14Z<p>Suchanart.apisaksiri: /* Physiology and Pathogenesis */</p>
<hr />
<div>{{Uncurated}}<br />
==Classification==<br />
<br />
Domain; Phylum; Class; Order; family [Others may be used. Use [http://www.ncbi.nlm.nih.gov/Taxonomy/ NCBI] link to find]<br />
<br />
===Species===<br />
<br />
{|<br />
| height="10" bgcolor="#FFDF95" |<br />
'''NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Tree&id=2&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy]'''<br />
|}<br />
<br />
''NS Mystery Organism''<br />
<br />
==Habitat Information ==<br />
Describe the location and conditions under which the organism was isolated.<br />
<br />
Date: 09/06/2017 <br />
<br />
Time: 18:49:00 CST<br />
<br />
Air Temperature: 83 ℉<br />
<br />
Humidity: 33%<br />
<br />
24 hr rainfall: 0 inches<br />
<br />
Pressure: 30.6 inch/ 1,017.0 mb<br />
<br />
Solar Radiation: 22.49 ms/m²<br />
<br />
Latitude: 30.144673 | Longitude: -97.841463<br />
<br />
The organism was isolated at a latitude of 30.144673 and a longitude of -97.841463 on September 6, 2017 at 18:49:00 CST. The conditions under which the NS Mystery Organism was isolated includes it being 83 degrees fahrenheit and 33% humidity. There were 0 inches of rainfall with a solar radiation of 22.49 ms/m^2.<br />
<br />
==Description and Significance==<br />
Describe the appearance (colonial and cellular), possible antimicrobial activity etc. of the organism, and why the organism might be significant.<br />
<br />
The colony morphology of NS Mystery Organism has a smooth surface with slightly raised elevation and an entire margin. The organism exhibits creamy yellow color. <br />
<br />
Cellular arrangement through microscopic observation is Diplobacilli. The antimicrobial activity test has shown a zone of inhibitions with all the antibiotics used. The Organism was also sensitized by some disinfectants used including Tea tree, PepperMint, Lysol 10% and Bleach 100%.<br />
<br />
==Genome Structure==<br />
<br />
Unknown due to complications from sequence reactions.<br />
<br />
==Cell Structure, Metabolism and Life Cycle==<br />
Interesting features of cell structure; how it gains energy; what important molecules it produces.<br />
<br />
<br />
NS Mystery Organism shows to be Gram-positive under Gram stain reaction test with prominent purple color from Crystal Violet. <br />
<br />
Under Capsule Stain test, the result is negative without any appearance of Capsule. The Organism also shows Negative Endospore test result under Endospore Stain test. However, under the Motility test, there was a movement away from the stab line into the medium which indicates a Positive Motility test result. <br />
<br />
Oxidase test has shown Positive result indicating Aerobic respiration. NS Mystery Organism turns yellow/yellow under Triple Sugar Iron Agar (TSI) test points out fermentation of Lactose and Glucose.<br />
<br />
The Organism produces DNase enzyme under DNA hydrolysis test with a clearing around growth in the agar plate. It also shows to have Lipase enzyme with a weak positive test result of Lipid Hydrolysis.<br />
<br />
==Physiology and Pathogenesis==<br />
Biochemical characteristics, enzymes made, other characteristics that may be used to identify the organism; contributions to environment (if any).<br><br />
If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.<br><br><br />
<br />
The Mannitol Salt Agar result for the organism indicated a positive result. The medium shows good yellow growth and is presumed Staphylococci because it is not inhibited by NaCl. The organism can produces acid from Mannitol fermentation and is presumed possible pathogenic Staphylococcus aureus. A gram reaction test was done and indicated that the organism was gram positive. In addition, a positive motility test and a positive DNA hydrolysis was tested. Non-motile organisms which lack flagella, are usually going to form a single line of growth that does not spread into the surrounding area. While a motile bacterium will grow and make a hazy zone around the stab line. Lipid hydrolysis was used to detect lipolytic bacteria, especially in high-fat dairy products. Lipase allows the organisms that produce it to break down lipids into smaller fragments. The test showed a weak positive. The NS Mystery Organism is aerobic, and can use oxygen as a terminal electron acceptor in respiration. This was proven through a positive oxidase test. EMB test is used to encourage the growth of fecal coliforms, to test drinking water. The result is based on lactose fermentation. The results were positive since pink colonies formed, indicating slow and weak fermentation.The organism is not inhibited by phenylethyl alcohol as proven with Phenylethyl Alcohol Agar.<br />
<br />
==References==<br />
[Sample reference] [http://ijs.sgmjournals.org/cgi/reprint/50/2/489 Takai, K., Sugai, A., Itoh, T., and Horikoshi, K. "''Palaeococcus ferrophilus'' gen. nov., sp. nov., a barophilic, hyperthermophilic archaeon from a deep-sea hydrothermal vent chimney". ''International Journal of Systematic and Evolutionary Microbiology''. 2000. Volume 50. p. 489-500.]<br />
<br />
==Author==<br />
Page authored by Suchanart Apisaksiri and Sally Phonthaasa, students of Prof. Kristine Hollingsworth at Austin Community College.<br />
<br />
<!-- Do not remove this line-->[[Category:Pages edited by students of Kristine Hollingsworth at Austin Community College]]</div>Suchanart.apisaksirihttps://microbewiki.kenyon.edu/index.php?title=NS_Mystery_Organism&diff=131950NS Mystery Organism2017-12-06T05:05:14Z<p>Suchanart.apisaksiri: /* Cell Structure, Metabolism and Life Cycle */</p>
<hr />
<div>{{Uncurated}}<br />
==Classification==<br />
<br />
Domain; Phylum; Class; Order; family [Others may be used. Use [http://www.ncbi.nlm.nih.gov/Taxonomy/ NCBI] link to find]<br />
<br />
===Species===<br />
<br />
{|<br />
| height="10" bgcolor="#FFDF95" |<br />
'''NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Tree&id=2&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy]'''<br />
|}<br />
<br />
''NS Mystery Organism''<br />
<br />
==Habitat Information ==<br />
Describe the location and conditions under which the organism was isolated.<br />
<br />
Date: 09/06/2017 <br />
<br />
Time: 18:49:00 CST<br />
<br />
Air Temperature: 83 ℉<br />
<br />
Humidity: 33%<br />
<br />
24 hr rainfall: 0 inches<br />
<br />
Pressure: 30.6 inch/ 1,017.0 mb<br />
<br />
Solar Radiation: 22.49 ms/m²<br />
<br />
Latitude: 30.144673 | Longitude: -97.841463<br />
<br />
The organism was isolated at a latitude of 30.144673 and a longitude of -97.841463 on September 6, 2017 at 18:49:00 CST. The conditions under which the NS Mystery Organism was isolated includes it being 83 degrees fahrenheit and 33% humidity. There were 0 inches of rainfall with a solar radiation of 22.49 ms/m^2.<br />
<br />
==Description and Significance==<br />
Describe the appearance (colonial and cellular), possible antimicrobial activity etc. of the organism, and why the organism might be significant.<br />
<br />
The colony morphology of NS Mystery Organism has a smooth surface with slightly raised elevation and an entire margin. The organism exhibits creamy yellow color. <br />
<br />
Cellular arrangement through microscopic observation is Diplobacilli. The antimicrobial activity test has shown a zone of inhibitions with all the antibiotics used. The Organism was also sensitized by some disinfectants used including Tea tree, PepperMint, Lysol 10% and Bleach 100%.<br />
<br />
==Genome Structure==<br />
<br />
Unknown due to complications from sequence reactions.<br />
<br />
==Cell Structure, Metabolism and Life Cycle==<br />
Interesting features of cell structure; how it gains energy; what important molecules it produces.<br />
<br />
<br />
NS Mystery Organism shows to be Gram-positive under Gram stain reaction test with prominent purple color from Crystal Violet. <br />
<br />
Under Capsule Stain test, the result is negative without any appearance of Capsule. The Organism also shows Negative Endospore test result under Endospore Stain test. However, under the Motility test, there was a movement away from the stab line into the medium which indicates a Positive Motility test result. <br />
<br />
Oxidase test has shown Positive result indicating Aerobic respiration. NS Mystery Organism turns yellow/yellow under Triple Sugar Iron Agar (TSI) test points out fermentation of Lactose and Glucose.<br />
<br />
The Organism produces DNase enzyme under DNA hydrolysis test with a clearing around growth in the agar plate. It also shows to have Lipase enzyme with a weak positive test result of Lipid Hydrolysis.<br />
<br />
==Physiology and Pathogenesis==<br />
Biochemical characteristics, enzymes made, other characteristics that may be used to identify the organism; contributions to environment (if any).<br><br />
If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.<br><br><br />
<br />
When a gram reaction test was performed, the results of the NS Mystery Organism resulted to be gram positive.<br />
<br />
==References==<br />
[Sample reference] [http://ijs.sgmjournals.org/cgi/reprint/50/2/489 Takai, K., Sugai, A., Itoh, T., and Horikoshi, K. "''Palaeococcus ferrophilus'' gen. nov., sp. nov., a barophilic, hyperthermophilic archaeon from a deep-sea hydrothermal vent chimney". ''International Journal of Systematic and Evolutionary Microbiology''. 2000. Volume 50. p. 489-500.]<br />
<br />
==Author==<br />
Page authored by Suchanart Apisaksiri and Sally Phonthaasa, students of Prof. Kristine Hollingsworth at Austin Community College.<br />
<br />
<!-- Do not remove this line-->[[Category:Pages edited by students of Kristine Hollingsworth at Austin Community College]]</div>Suchanart.apisaksirihttps://microbewiki.kenyon.edu/index.php?title=NS_Mystery_Organism&diff=131949NS Mystery Organism2017-12-06T04:56:16Z<p>Suchanart.apisaksiri: /* Physiology and Pathogenesis */</p>
<hr />
<div>{{Uncurated}}<br />
==Classification==<br />
<br />
Domain; Phylum; Class; Order; family [Others may be used. Use [http://www.ncbi.nlm.nih.gov/Taxonomy/ NCBI] link to find]<br />
<br />
===Species===<br />
<br />
{|<br />
| height="10" bgcolor="#FFDF95" |<br />
'''NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Tree&id=2&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy]'''<br />
|}<br />
<br />
''NS Mystery Organism''<br />
<br />
==Habitat Information ==<br />
Describe the location and conditions under which the organism was isolated.<br />
<br />
Date: 09/06/2017 <br />
<br />
Time: 18:49:00 CST<br />
<br />
Air Temperature: 83 ℉<br />
<br />
Humidity: 33%<br />
<br />
24 hr rainfall: 0 inches<br />
<br />
Pressure: 30.6 inch/ 1,017.0 mb<br />
<br />
Solar Radiation: 22.49 ms/m²<br />
<br />
Latitude: 30.144673 | Longitude: -97.841463<br />
<br />
The organism was isolated at a latitude of 30.144673 and a longitude of -97.841463 on September 6, 2017 at 18:49:00 CST. The conditions under which the NS Mystery Organism was isolated includes it being 83 degrees fahrenheit and 33% humidity. There were 0 inches of rainfall with a solar radiation of 22.49 ms/m^2.<br />
<br />
==Description and Significance==<br />
Describe the appearance (colonial and cellular), possible antimicrobial activity etc. of the organism, and why the organism might be significant.<br />
<br />
The colony morphology of NS Mystery Organism has a smooth surface with slightly raised elevation and an entire margin. The organism exhibits creamy yellow color. <br />
<br />
Cellular arrangement through microscopic observation is Diplobacilli. The antimicrobial activity test has shown a zone of inhibitions with all the antibiotics used. The Organism was also sensitized by some disinfectants used including Tea tree, PepperMint, Lysol 10% and Bleach 100%.<br />
<br />
==Genome Structure==<br />
<br />
Unknown due to complications from sequence reactions.<br />
<br />
==Cell Structure, Metabolism and Life Cycle==<br />
Interesting features of cell structure; how it gains energy; what important molecules it produces.<br />
<br />
<br />
==Physiology and Pathogenesis==<br />
Biochemical characteristics, enzymes made, other characteristics that may be used to identify the organism; contributions to environment (if any).<br><br />
If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.<br><br><br />
<br />
When a gram reaction test was performed, the results of the NS Mystery Organism resulted to be gram positive.<br />
<br />
==References==<br />
[Sample reference] [http://ijs.sgmjournals.org/cgi/reprint/50/2/489 Takai, K., Sugai, A., Itoh, T., and Horikoshi, K. "''Palaeococcus ferrophilus'' gen. nov., sp. nov., a barophilic, hyperthermophilic archaeon from a deep-sea hydrothermal vent chimney". ''International Journal of Systematic and Evolutionary Microbiology''. 2000. Volume 50. p. 489-500.]<br />
<br />
==Author==<br />
Page authored by Suchanart Apisaksiri and Sally Phonthaasa, students of Prof. Kristine Hollingsworth at Austin Community College.<br />
<br />
<!-- Do not remove this line-->[[Category:Pages edited by students of Kristine Hollingsworth at Austin Community College]]</div>Suchanart.apisaksirihttps://microbewiki.kenyon.edu/index.php?title=NS_Mystery_Organism&diff=131944NS Mystery Organism2017-12-06T04:41:19Z<p>Suchanart.apisaksiri: /* Genome Structure */</p>
<hr />
<div>{{Uncurated}}<br />
==Classification==<br />
<br />
Domain; Phylum; Class; Order; family [Others may be used. Use [http://www.ncbi.nlm.nih.gov/Taxonomy/ NCBI] link to find]<br />
<br />
===Species===<br />
<br />
{|<br />
| height="10" bgcolor="#FFDF95" |<br />
'''NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Tree&id=2&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy]'''<br />
|}<br />
<br />
''NS Mystery Organism''<br />
<br />
==Habitat Information ==<br />
Describe the location and conditions under which the organism was isolated.<br />
<br />
Date: 09/06/2017 <br />
<br />
Time: 18:49:00 CST<br />
<br />
Air Temperature: 83 ℉<br />
<br />
Humidity: 33%<br />
<br />
24 hr rainfall: 0 inches<br />
<br />
Pressure: 30.6 inch/ 1,017.0 mb<br />
<br />
Solar Radiation: 22.49 ms/m²<br />
<br />
Latitude: 30.144673 | Longitude: -97.841463<br />
<br />
The organism was isolated at a latitude of 30.144673 and a longitude of -97.841463 on September 6, 2017 at 18:49:00 CST. The conditions under which the NS Mystery Organism was isolated includes it being 83 degrees fahrenheit and 33% humidity. There were 0 inches of rainfall with a solar radiation of 22.49 ms/m^2.<br />
<br />
==Description and Significance==<br />
Describe the appearance (colonial and cellular), possible antimicrobial activity etc. of the organism, and why the organism might be significant.<br />
<br />
The colony morphology of NS Mystery Organism has a smooth surface with slightly raised elevation and an entire margin. The organism exhibits creamy yellow color. <br />
<br />
Cellular arrangement through microscopic observation is Diplobacilli. The antimicrobial activity test has shown a zone of inhibitions with all the antibiotics used. The Organism was also sensitized by some disinfectants used including Tea tree, PepperMint, Lysol 10% and Bleach 100%.<br />
<br />
==Genome Structure==<br />
<br />
Unknown due to complications from sequence reactions.<br />
<br />
==Cell Structure, Metabolism and Life Cycle==<br />
Interesting features of cell structure; how it gains energy; what important molecules it produces.<br />
<br />
<br />
==Physiology and Pathogenesis==<br />
Biochemical characteristics, enzymes made, other characteristics that may be used to identify the organism; contributions to environment (if any).<br><br />
If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.<br><br><br />
<br />
==References==<br />
[Sample reference] [http://ijs.sgmjournals.org/cgi/reprint/50/2/489 Takai, K., Sugai, A., Itoh, T., and Horikoshi, K. "''Palaeococcus ferrophilus'' gen. nov., sp. nov., a barophilic, hyperthermophilic archaeon from a deep-sea hydrothermal vent chimney". ''International Journal of Systematic and Evolutionary Microbiology''. 2000. Volume 50. p. 489-500.]<br />
<br />
==Author==<br />
Page authored by Suchanart Apisaksiri and Sally Phonthaasa, students of Prof. Kristine Hollingsworth at Austin Community College.<br />
<br />
<!-- Do not remove this line-->[[Category:Pages edited by students of Kristine Hollingsworth at Austin Community College]]</div>Suchanart.apisaksirihttps://microbewiki.kenyon.edu/index.php?title=NS_Mystery_Organism&diff=131943NS Mystery Organism2017-12-06T04:39:25Z<p>Suchanart.apisaksiri: /* Description and Significance */</p>
<hr />
<div>{{Uncurated}}<br />
==Classification==<br />
<br />
Domain; Phylum; Class; Order; family [Others may be used. Use [http://www.ncbi.nlm.nih.gov/Taxonomy/ NCBI] link to find]<br />
<br />
===Species===<br />
<br />
{|<br />
| height="10" bgcolor="#FFDF95" |<br />
'''NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Tree&id=2&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy]'''<br />
|}<br />
<br />
''NS Mystery Organism''<br />
<br />
==Habitat Information ==<br />
Describe the location and conditions under which the organism was isolated.<br />
<br />
Date: 09/06/2017 <br />
<br />
Time: 18:49:00 CST<br />
<br />
Air Temperature: 83 ℉<br />
<br />
Humidity: 33%<br />
<br />
24 hr rainfall: 0 inches<br />
<br />
Pressure: 30.6 inch/ 1,017.0 mb<br />
<br />
Solar Radiation: 22.49 ms/m²<br />
<br />
Latitude: 30.144673 | Longitude: -97.841463<br />
<br />
The organism was isolated at a latitude of 30.144673 and a longitude of -97.841463 on September 6, 2017 at 18:49:00 CST. The conditions under which the NS Mystery Organism was isolated includes it being 83 degrees fahrenheit and 33% humidity. There were 0 inches of rainfall with a solar radiation of 22.49 ms/m^2.<br />
<br />
==Description and Significance==<br />
Describe the appearance (colonial and cellular), possible antimicrobial activity etc. of the organism, and why the organism might be significant.<br />
<br />
The colony morphology of NS Mystery Organism has a smooth surface with slightly raised elevation and an entire margin. The organism exhibits creamy yellow color. <br />
<br />
Cellular arrangement through microscopic observation is Diplobacilli. The antimicrobial activity test has shown a zone of inhibitions with all the antibiotics used. The Organism was also sensitized by some disinfectants used including Tea tree, PepperMint, Lysol 10% and Bleach 100%.<br />
<br />
==Genome Structure==<br />
<br />
Unknown<br />
<br />
==Cell Structure, Metabolism and Life Cycle==<br />
Interesting features of cell structure; how it gains energy; what important molecules it produces.<br />
<br />
<br />
==Physiology and Pathogenesis==<br />
Biochemical characteristics, enzymes made, other characteristics that may be used to identify the organism; contributions to environment (if any).<br><br />
If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.<br><br><br />
<br />
==References==<br />
[Sample reference] [http://ijs.sgmjournals.org/cgi/reprint/50/2/489 Takai, K., Sugai, A., Itoh, T., and Horikoshi, K. "''Palaeococcus ferrophilus'' gen. nov., sp. nov., a barophilic, hyperthermophilic archaeon from a deep-sea hydrothermal vent chimney". ''International Journal of Systematic and Evolutionary Microbiology''. 2000. Volume 50. p. 489-500.]<br />
<br />
==Author==<br />
Page authored by Suchanart Apisaksiri and Sally Phonthaasa, students of Prof. Kristine Hollingsworth at Austin Community College.<br />
<br />
<!-- Do not remove this line-->[[Category:Pages edited by students of Kristine Hollingsworth at Austin Community College]]</div>Suchanart.apisaksirihttps://microbewiki.kenyon.edu/index.php?title=NS_Mystery_Organism&diff=131942NS Mystery Organism2017-12-06T04:39:04Z<p>Suchanart.apisaksiri: /* Description and Significance */</p>
<hr />
<div>{{Uncurated}}<br />
==Classification==<br />
<br />
Domain; Phylum; Class; Order; family [Others may be used. Use [http://www.ncbi.nlm.nih.gov/Taxonomy/ NCBI] link to find]<br />
<br />
===Species===<br />
<br />
{|<br />
| height="10" bgcolor="#FFDF95" |<br />
'''NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Tree&id=2&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy]'''<br />
|}<br />
<br />
''NS Mystery Organism''<br />
<br />
==Habitat Information ==<br />
Describe the location and conditions under which the organism was isolated.<br />
<br />
Date: 09/06/2017 <br />
<br />
Time: 18:49:00 CST<br />
<br />
Air Temperature: 83 ℉<br />
<br />
Humidity: 33%<br />
<br />
24 hr rainfall: 0 inches<br />
<br />
Pressure: 30.6 inch/ 1,017.0 mb<br />
<br />
Solar Radiation: 22.49 ms/m²<br />
<br />
Latitude: 30.144673 | Longitude: -97.841463<br />
<br />
The organism was isolated at a latitude of 30.144673 and a longitude of -97.841463 on September 6, 2017 at 18:49:00 CST. The conditions under which the NS Mystery Organism was isolated includes it being 83 degrees fahrenheit and 33% humidity. There were 0 inches of rainfall with a solar radiation of 22.49 ms/m^2.<br />
<br />
==Description and Significance==<br />
Describe the appearance (colonial and cellular), possible antimicrobial activity etc. of the organism, and why the organism might be significant.<br />
The colony morphology of NS Mystery Organism has a smooth surface with slightly raised elevation and an entire margin. The organism exhibits creamy yellow color. <br />
<br />
Cellular arrangement through microscopic observation is Diplobacilli. The antimicrobial activity test has shown a zone of inhibitions with all the antibiotics used. The Organism was also sensitized by some disinfectants used including Tea tree, PepperMint, Lysol 10% and Bleach 100%.<br />
<br />
==Genome Structure==<br />
<br />
Unknown<br />
<br />
==Cell Structure, Metabolism and Life Cycle==<br />
Interesting features of cell structure; how it gains energy; what important molecules it produces.<br />
<br />
<br />
==Physiology and Pathogenesis==<br />
Biochemical characteristics, enzymes made, other characteristics that may be used to identify the organism; contributions to environment (if any).<br><br />
If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.<br><br><br />
<br />
==References==<br />
[Sample reference] [http://ijs.sgmjournals.org/cgi/reprint/50/2/489 Takai, K., Sugai, A., Itoh, T., and Horikoshi, K. "''Palaeococcus ferrophilus'' gen. nov., sp. nov., a barophilic, hyperthermophilic archaeon from a deep-sea hydrothermal vent chimney". ''International Journal of Systematic and Evolutionary Microbiology''. 2000. Volume 50. p. 489-500.]<br />
<br />
==Author==<br />
Page authored by Suchanart Apisaksiri and Sally Phonthaasa, students of Prof. Kristine Hollingsworth at Austin Community College.<br />
<br />
<!-- Do not remove this line-->[[Category:Pages edited by students of Kristine Hollingsworth at Austin Community College]]</div>Suchanart.apisaksirihttps://microbewiki.kenyon.edu/index.php?title=NS_Mystery_Organism&diff=131940NS Mystery Organism2017-12-06T04:38:06Z<p>Suchanart.apisaksiri: /* Genome Structure */</p>
<hr />
<div>{{Uncurated}}<br />
==Classification==<br />
<br />
Domain; Phylum; Class; Order; family [Others may be used. Use [http://www.ncbi.nlm.nih.gov/Taxonomy/ NCBI] link to find]<br />
<br />
===Species===<br />
<br />
{|<br />
| height="10" bgcolor="#FFDF95" |<br />
'''NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Tree&id=2&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy]'''<br />
|}<br />
<br />
''NS Mystery Organism''<br />
<br />
==Habitat Information ==<br />
Describe the location and conditions under which the organism was isolated.<br />
<br />
Date: 09/06/2017 <br />
<br />
Time: 18:49:00 CST<br />
<br />
Air Temperature: 83 ℉<br />
<br />
Humidity: 33%<br />
<br />
24 hr rainfall: 0 inches<br />
<br />
Pressure: 30.6 inch/ 1,017.0 mb<br />
<br />
Solar Radiation: 22.49 ms/m²<br />
<br />
Latitude: 30.144673 | Longitude: -97.841463<br />
<br />
The organism was isolated at a latitude of 30.144673 and a longitude of -97.841463 on September 6, 2017 at 18:49:00 CST. The conditions under which the NS Mystery Organism was isolated includes it being 83 degrees fahrenheit and 33% humidity. There were 0 inches of rainfall with a solar radiation of 22.49 ms/m^2.<br />
<br />
==Description and Significance==<br />
Describe the appearance (colonial and cellular), possible antimicrobial activity etc. of the organism, and why the organism might be significant.<br />
<br />
==Genome Structure==<br />
<br />
Unknown<br />
<br />
==Cell Structure, Metabolism and Life Cycle==<br />
Interesting features of cell structure; how it gains energy; what important molecules it produces.<br />
<br />
<br />
==Physiology and Pathogenesis==<br />
Biochemical characteristics, enzymes made, other characteristics that may be used to identify the organism; contributions to environment (if any).<br><br />
If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.<br><br><br />
<br />
==References==<br />
[Sample reference] [http://ijs.sgmjournals.org/cgi/reprint/50/2/489 Takai, K., Sugai, A., Itoh, T., and Horikoshi, K. "''Palaeococcus ferrophilus'' gen. nov., sp. nov., a barophilic, hyperthermophilic archaeon from a deep-sea hydrothermal vent chimney". ''International Journal of Systematic and Evolutionary Microbiology''. 2000. Volume 50. p. 489-500.]<br />
<br />
==Author==<br />
Page authored by Suchanart Apisaksiri and Sally Phonthaasa, students of Prof. Kristine Hollingsworth at Austin Community College.<br />
<br />
<!-- Do not remove this line-->[[Category:Pages edited by students of Kristine Hollingsworth at Austin Community College]]</div>Suchanart.apisaksirihttps://microbewiki.kenyon.edu/index.php?title=NS_Mystery_Organism&diff=131938NS Mystery Organism2017-12-06T04:36:06Z<p>Suchanart.apisaksiri: /* Classification */</p>
<hr />
<div>{{Uncurated}}<br />
==Classification==<br />
<br />
Domain; Phylum; Class; Order; family [Others may be used. Use [http://www.ncbi.nlm.nih.gov/Taxonomy/ NCBI] link to find]<br />
<br />
===Species===<br />
<br />
{|<br />
| height="10" bgcolor="#FFDF95" |<br />
'''NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Tree&id=2&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy]'''<br />
|}<br />
<br />
''NS Mystery Organism''<br />
<br />
==Habitat Information ==<br />
Describe the location and conditions under which the organism was isolated.<br />
<br />
Date: 09/06/2017 <br />
<br />
Time: 18:49:00 CST<br />
<br />
Air Temperature: 83 ℉<br />
<br />
Humidity: 33%<br />
<br />
24 hr rainfall: 0 inches<br />
<br />
Pressure: 30.6 inch/ 1,017.0 mb<br />
<br />
Solar Radiation: 22.49 ms/m²<br />
<br />
Latitude: 30.144673 | Longitude: -97.841463<br />
<br />
The organism was isolated at a latitude of 30.144673 and a longitude of -97.841463 on September 6, 2017 at 18:49:00 CST. The conditions under which the NS Mystery Organism was isolated includes it being 83 degrees fahrenheit and 33% humidity. There were 0 inches of rainfall with a solar radiation of 22.49 ms/m^2.<br />
<br />
==Description and Significance==<br />
Describe the appearance (colonial and cellular), possible antimicrobial activity etc. of the organism, and why the organism might be significant.<br />
<br />
==Genome Structure==<br />
Due to complications with PCR results, the sequence of the organism is unknown.<br />
<br />
==Cell Structure, Metabolism and Life Cycle==<br />
Interesting features of cell structure; how it gains energy; what important molecules it produces.<br />
<br />
<br />
==Physiology and Pathogenesis==<br />
Biochemical characteristics, enzymes made, other characteristics that may be used to identify the organism; contributions to environment (if any).<br><br />
If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.<br><br><br />
<br />
==References==<br />
[Sample reference] [http://ijs.sgmjournals.org/cgi/reprint/50/2/489 Takai, K., Sugai, A., Itoh, T., and Horikoshi, K. "''Palaeococcus ferrophilus'' gen. nov., sp. nov., a barophilic, hyperthermophilic archaeon from a deep-sea hydrothermal vent chimney". ''International Journal of Systematic and Evolutionary Microbiology''. 2000. Volume 50. p. 489-500.]<br />
<br />
==Author==<br />
Page authored by Suchanart Apisaksiri and Sally Phonthaasa, students of Prof. Kristine Hollingsworth at Austin Community College.<br />
<br />
<!-- Do not remove this line-->[[Category:Pages edited by students of Kristine Hollingsworth at Austin Community College]]</div>Suchanart.apisaksirihttps://microbewiki.kenyon.edu/index.php?title=NS_Mystery_Organism&diff=131937NS Mystery Organism2017-12-06T04:35:12Z<p>Suchanart.apisaksiri: /* Genome Structure */</p>
<hr />
<div>{{Uncurated}}<br />
==Classification==<br />
<br />
Domain; Phylum; Class; Order; family [Others may be used. Use [http://www.ncbi.nlm.nih.gov/Taxonomy/ NCBI] link to find]<br />
<br />
===Species===<br />
<br />
{|<br />
| height="10" bgcolor="#FFDF95" |<br />
'''NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Tree&id=2&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy]'''<br />
|}<br />
<br />
''Genus species''<br />
<br />
==Habitat Information ==<br />
Describe the location and conditions under which the organism was isolated.<br />
<br />
Date: 09/06/2017 <br />
<br />
Time: 18:49:00 CST<br />
<br />
Air Temperature: 83 ℉<br />
<br />
Humidity: 33%<br />
<br />
24 hr rainfall: 0 inches<br />
<br />
Pressure: 30.6 inch/ 1,017.0 mb<br />
<br />
Solar Radiation: 22.49 ms/m²<br />
<br />
Latitude: 30.144673 | Longitude: -97.841463<br />
<br />
The organism was isolated at a latitude of 30.144673 and a longitude of -97.841463 on September 6, 2017 at 18:49:00 CST. The conditions under which the NS Mystery Organism was isolated includes it being 83 degrees fahrenheit and 33% humidity. There were 0 inches of rainfall with a solar radiation of 22.49 ms/m^2.<br />
<br />
==Description and Significance==<br />
Describe the appearance (colonial and cellular), possible antimicrobial activity etc. of the organism, and why the organism might be significant.<br />
<br />
==Genome Structure==<br />
Due to complications with PCR results, the sequence of the organism is unknown.<br />
<br />
==Cell Structure, Metabolism and Life Cycle==<br />
Interesting features of cell structure; how it gains energy; what important molecules it produces.<br />
<br />
<br />
==Physiology and Pathogenesis==<br />
Biochemical characteristics, enzymes made, other characteristics that may be used to identify the organism; contributions to environment (if any).<br><br />
If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.<br><br><br />
<br />
==References==<br />
[Sample reference] [http://ijs.sgmjournals.org/cgi/reprint/50/2/489 Takai, K., Sugai, A., Itoh, T., and Horikoshi, K. "''Palaeococcus ferrophilus'' gen. nov., sp. nov., a barophilic, hyperthermophilic archaeon from a deep-sea hydrothermal vent chimney". ''International Journal of Systematic and Evolutionary Microbiology''. 2000. Volume 50. p. 489-500.]<br />
<br />
==Author==<br />
Page authored by Suchanart Apisaksiri and Sally Phonthaasa, students of Prof. Kristine Hollingsworth at Austin Community College.<br />
<br />
<!-- Do not remove this line-->[[Category:Pages edited by students of Kristine Hollingsworth at Austin Community College]]</div>Suchanart.apisaksirihttps://microbewiki.kenyon.edu/index.php?title=NS_Mystery_Organism&diff=131936NS Mystery Organism2017-12-06T04:32:27Z<p>Suchanart.apisaksiri: /* Habitat Information */</p>
<hr />
<div>{{Uncurated}}<br />
==Classification==<br />
<br />
Domain; Phylum; Class; Order; family [Others may be used. Use [http://www.ncbi.nlm.nih.gov/Taxonomy/ NCBI] link to find]<br />
<br />
===Species===<br />
<br />
{|<br />
| height="10" bgcolor="#FFDF95" |<br />
'''NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Tree&id=2&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy]'''<br />
|}<br />
<br />
''Genus species''<br />
<br />
==Habitat Information ==<br />
Describe the location and conditions under which the organism was isolated.<br />
<br />
Date: 09/06/2017 <br />
<br />
Time: 18:49:00 CST<br />
<br />
Air Temperature: 83 ℉<br />
<br />
Humidity: 33%<br />
<br />
24 hr rainfall: 0 inches<br />
<br />
Pressure: 30.6 inch/ 1,017.0 mb<br />
<br />
Solar Radiation: 22.49 ms/m²<br />
<br />
Latitude: 30.144673 | Longitude: -97.841463<br />
<br />
The organism was isolated at a latitude of 30.144673 and a longitude of -97.841463 on September 6, 2017 at 18:49:00 CST. The conditions under which the NS Mystery Organism was isolated includes it being 83 degrees fahrenheit and 33% humidity. There were 0 inches of rainfall with a solar radiation of 22.49 ms/m^2.<br />
<br />
==Description and Significance==<br />
Describe the appearance (colonial and cellular), possible antimicrobial activity etc. of the organism, and why the organism might be significant.<br />
<br />
==Genome Structure==<br />
Describe the size and content of the genome. How many chromosomes? Circular or linear? Other interesting features? What is known about its sequence? Include S Ribosomal sequence that you obtained from PCR and sequencing here.<br />
<br />
Unknown<br />
<br />
==Cell Structure, Metabolism and Life Cycle==<br />
Interesting features of cell structure; how it gains energy; what important molecules it produces.<br />
<br />
<br />
==Physiology and Pathogenesis==<br />
Biochemical characteristics, enzymes made, other characteristics that may be used to identify the organism; contributions to environment (if any).<br><br />
If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.<br><br><br />
<br />
==References==<br />
[Sample reference] [http://ijs.sgmjournals.org/cgi/reprint/50/2/489 Takai, K., Sugai, A., Itoh, T., and Horikoshi, K. "''Palaeococcus ferrophilus'' gen. nov., sp. nov., a barophilic, hyperthermophilic archaeon from a deep-sea hydrothermal vent chimney". ''International Journal of Systematic and Evolutionary Microbiology''. 2000. Volume 50. p. 489-500.]<br />
<br />
==Author==<br />
Page authored by Suchanart Apisaksiri and Sally Phonthaasa, students of Prof. Kristine Hollingsworth at Austin Community College.<br />
<br />
<!-- Do not remove this line-->[[Category:Pages edited by students of Kristine Hollingsworth at Austin Community College]]</div>Suchanart.apisaksirihttps://microbewiki.kenyon.edu/index.php?title=NS_Mystery_Organism&diff=131935NS Mystery Organism2017-12-06T04:27:37Z<p>Suchanart.apisaksiri: /* Genome Structure */</p>
<hr />
<div>{{Uncurated}}<br />
==Classification==<br />
<br />
Domain; Phylum; Class; Order; family [Others may be used. Use [http://www.ncbi.nlm.nih.gov/Taxonomy/ NCBI] link to find]<br />
<br />
===Species===<br />
<br />
{|<br />
| height="10" bgcolor="#FFDF95" |<br />
'''NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Tree&id=2&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy]'''<br />
|}<br />
<br />
''Genus species''<br />
<br />
==Habitat Information ==<br />
Describe the location and conditions under which the organism was isolated.<br />
<br />
Date: 09/06/2017 Time: 18:49:00 CST<br />
Air Temperature: 83 ℉<br />
Humidity: 33%<br />
24 hr rainfall: 0 inches<br />
Pressure: 30.6 inch/ 1,017.0 mb<br />
Solar Radiation: 22.49 ms/m²<br />
Latitude: 30.144673 | Longitude: -97.841463<br />
<br />
The organism was isolated at a latitude of 30.144673 and a longitude of -97.841463 on September 6, 2017 at 18:49:00 CST. The conditions under which the NS Mystery Organism was isolated includes it being 83 degrees fahrenheit and 33% humidity. There were 0 inches of rainfall with a solar radiation of 22.49 ms/m^2.<br />
<br />
==Description and Significance==<br />
Describe the appearance (colonial and cellular), possible antimicrobial activity etc. of the organism, and why the organism might be significant.<br />
<br />
==Genome Structure==<br />
Describe the size and content of the genome. How many chromosomes? Circular or linear? Other interesting features? What is known about its sequence? Include S Ribosomal sequence that you obtained from PCR and sequencing here.<br />
<br />
Unknown<br />
<br />
==Cell Structure, Metabolism and Life Cycle==<br />
Interesting features of cell structure; how it gains energy; what important molecules it produces.<br />
<br />
<br />
==Physiology and Pathogenesis==<br />
Biochemical characteristics, enzymes made, other characteristics that may be used to identify the organism; contributions to environment (if any).<br><br />
If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.<br><br><br />
<br />
==References==<br />
[Sample reference] [http://ijs.sgmjournals.org/cgi/reprint/50/2/489 Takai, K., Sugai, A., Itoh, T., and Horikoshi, K. "''Palaeococcus ferrophilus'' gen. nov., sp. nov., a barophilic, hyperthermophilic archaeon from a deep-sea hydrothermal vent chimney". ''International Journal of Systematic and Evolutionary Microbiology''. 2000. Volume 50. p. 489-500.]<br />
<br />
==Author==<br />
Page authored by Suchanart Apisaksiri and Sally Phonthaasa, students of Prof. Kristine Hollingsworth at Austin Community College.<br />
<br />
<!-- Do not remove this line-->[[Category:Pages edited by students of Kristine Hollingsworth at Austin Community College]]</div>Suchanart.apisaksirihttps://microbewiki.kenyon.edu/index.php?title=NS_Mystery_Organism&diff=131934NS Mystery Organism2017-12-06T04:24:25Z<p>Suchanart.apisaksiri: /* Habitat Information */</p>
<hr />
<div>{{Uncurated}}<br />
==Classification==<br />
<br />
Domain; Phylum; Class; Order; family [Others may be used. Use [http://www.ncbi.nlm.nih.gov/Taxonomy/ NCBI] link to find]<br />
<br />
===Species===<br />
<br />
{|<br />
| height="10" bgcolor="#FFDF95" |<br />
'''NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Tree&id=2&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy]'''<br />
|}<br />
<br />
''Genus species''<br />
<br />
==Habitat Information ==<br />
Describe the location and conditions under which the organism was isolated.<br />
<br />
Date: 09/06/2017 Time: 18:49:00 CST<br />
Air Temperature: 83 ℉<br />
Humidity: 33%<br />
24 hr rainfall: 0 inches<br />
Pressure: 30.6 inch/ 1,017.0 mb<br />
Solar Radiation: 22.49 ms/m²<br />
Latitude: 30.144673 | Longitude: -97.841463<br />
<br />
The organism was isolated at a latitude of 30.144673 and a longitude of -97.841463 on September 6, 2017 at 18:49:00 CST. The conditions under which the NS Mystery Organism was isolated includes it being 83 degrees fahrenheit and 33% humidity. There were 0 inches of rainfall with a solar radiation of 22.49 ms/m^2.<br />
<br />
==Description and Significance==<br />
Describe the appearance (colonial and cellular), possible antimicrobial activity etc. of the organism, and why the organism might be significant.<br />
<br />
==Genome Structure==<br />
Describe the size and content of the genome. How many chromosomes? Circular or linear? Other interesting features? What is known about its sequence? Include S Ribosomal sequence that you obtained from PCR and sequencing here.<br />
<br />
<br />
==Cell Structure, Metabolism and Life Cycle==<br />
Interesting features of cell structure; how it gains energy; what important molecules it produces.<br />
<br />
<br />
==Physiology and Pathogenesis==<br />
Biochemical characteristics, enzymes made, other characteristics that may be used to identify the organism; contributions to environment (if any).<br><br />
If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.<br><br><br />
<br />
==References==<br />
[Sample reference] [http://ijs.sgmjournals.org/cgi/reprint/50/2/489 Takai, K., Sugai, A., Itoh, T., and Horikoshi, K. "''Palaeococcus ferrophilus'' gen. nov., sp. nov., a barophilic, hyperthermophilic archaeon from a deep-sea hydrothermal vent chimney". ''International Journal of Systematic and Evolutionary Microbiology''. 2000. Volume 50. p. 489-500.]<br />
<br />
==Author==<br />
Page authored by Suchanart Apisaksiri and Sally Phonthaasa, students of Prof. Kristine Hollingsworth at Austin Community College.<br />
<br />
<!-- Do not remove this line-->[[Category:Pages edited by students of Kristine Hollingsworth at Austin Community College]]</div>Suchanart.apisaksirihttps://microbewiki.kenyon.edu/index.php?title=NS_Mystery_Organism&diff=131933NS Mystery Organism2017-12-06T04:04:31Z<p>Suchanart.apisaksiri: /* Author */</p>
<hr />
<div>{{Uncurated}}<br />
==Classification==<br />
<br />
Domain; Phylum; Class; Order; family [Others may be used. Use [http://www.ncbi.nlm.nih.gov/Taxonomy/ NCBI] link to find]<br />
<br />
===Species===<br />
<br />
{|<br />
| height="10" bgcolor="#FFDF95" |<br />
'''NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Tree&id=2&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy]'''<br />
|}<br />
<br />
''Genus species''<br />
<br />
==Habitat Information ==<br />
Describe the location and conditions under which the organism was isolated.<br />
<br />
==Description and Significance==<br />
Describe the appearance (colonial and cellular), possible antimicrobial activity etc. of the organism, and why the organism might be significant.<br />
<br />
==Genome Structure==<br />
Describe the size and content of the genome. How many chromosomes? Circular or linear? Other interesting features? What is known about its sequence? Include S Ribosomal sequence that you obtained from PCR and sequencing here.<br />
<br />
<br />
==Cell Structure, Metabolism and Life Cycle==<br />
Interesting features of cell structure; how it gains energy; what important molecules it produces.<br />
<br />
<br />
==Physiology and Pathogenesis==<br />
Biochemical characteristics, enzymes made, other characteristics that may be used to identify the organism; contributions to environment (if any).<br><br />
If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.<br><br><br />
<br />
==References==<br />
[Sample reference] [http://ijs.sgmjournals.org/cgi/reprint/50/2/489 Takai, K., Sugai, A., Itoh, T., and Horikoshi, K. "''Palaeococcus ferrophilus'' gen. nov., sp. nov., a barophilic, hyperthermophilic archaeon from a deep-sea hydrothermal vent chimney". ''International Journal of Systematic and Evolutionary Microbiology''. 2000. Volume 50. p. 489-500.]<br />
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==Author==<br />
Page authored by Suchanart Apisaksiri and Sally Phonthaasa, students of Prof. Kristine Hollingsworth at Austin Community College.<br />
<br />
<!-- Do not remove this line-->[[Category:Pages edited by students of Kristine Hollingsworth at Austin Community College]]</div>Suchanart.apisaksirihttps://microbewiki.kenyon.edu/index.php?title=NS_Mystery_Organism&diff=131776NS Mystery Organism2017-12-01T21:52:32Z<p>Suchanart.apisaksiri: Created page with "{{Uncurated}} ==Classification== Domain; Phylum; Class; Order; family [Others may be used. Use [http://www.ncbi.nlm.nih.gov/Taxonomy/ NCBI] link to find] ===Species=== {|..."</p>
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<div>{{Uncurated}}<br />
==Classification==<br />
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Domain; Phylum; Class; Order; family [Others may be used. Use [http://www.ncbi.nlm.nih.gov/Taxonomy/ NCBI] link to find]<br />
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===Species===<br />
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{|<br />
| height="10" bgcolor="#FFDF95" |<br />
'''NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Tree&id=2&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy]'''<br />
|}<br />
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''Genus species''<br />
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==Habitat Information ==<br />
Describe the location and conditions under which the organism was isolated.<br />
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==Description and Significance==<br />
Describe the appearance (colonial and cellular), possible antimicrobial activity etc. of the organism, and why the organism might be significant.<br />
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==Genome Structure==<br />
Describe the size and content of the genome. How many chromosomes? Circular or linear? Other interesting features? What is known about its sequence? Include S Ribosomal sequence that you obtained from PCR and sequencing here.<br />
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==Cell Structure, Metabolism and Life Cycle==<br />
Interesting features of cell structure; how it gains energy; what important molecules it produces.<br />
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==Physiology and Pathogenesis==<br />
Biochemical characteristics, enzymes made, other characteristics that may be used to identify the organism; contributions to environment (if any).<br><br />
If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.<br><br><br />
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==References==<br />
[Sample reference] [http://ijs.sgmjournals.org/cgi/reprint/50/2/489 Takai, K., Sugai, A., Itoh, T., and Horikoshi, K. "''Palaeococcus ferrophilus'' gen. nov., sp. nov., a barophilic, hyperthermophilic archaeon from a deep-sea hydrothermal vent chimney". ''International Journal of Systematic and Evolutionary Microbiology''. 2000. Volume 50. p. 489-500.]<br />
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==Author==<br />
Page authored by _____, student of Prof. Kristine Hollingsworth at Austin Community College.<br />
<br />
<!-- Do not remove this line-->[[Category:Pages edited by students of Kristine Hollingsworth at Austin Community College]]</div>Suchanart.apisaksiri