File:Southernblottanalysis.png
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Only one mating type gene pair remains in the somatic nucleus. Southern blot analysis was carried out using whole-cell genomic DNA from a mature strain of each mating type (SB4208, SB4211, SB4214, SB4217, SB4220, and SB4223; see Table S1). The DNA was digested with PvuII restriction endonuclease and separated by pulsed-field gel electrophoresis. Black segments, mating type-specific segment of each gene pair; diagonally hatched segments, conserved TM exons; arrows, PvuII sites; thin black bars, probes; size (kb) shown is that of the relevant PvuII fragment in the somatic genome (the corresponding germline PvuII fragments are not visible due to differences in size and copy number).doi:10.1371/journal.pbio.1001518.g004
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
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| current | 21:58, 8 May 2013 | | 1,944 × 1,707 (836 KB) | Bellk (talk | contribs) | Only one mating type gene pair remains in the somatic nucleus. Southern blot analysis was carried out using whole-cell genomic DNA from a mature strain of each mating type (SB4208, SB4211, SB4214, SB4217, SB4220, and SB4223; see Table S1). The DNA was ... |
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