Pontibacter salisaro

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Classification

Domain: Bacteria

Kingdom: Bscterioldetes/chlorobi

Phylum: Bacteroidetes

Class: Cytophagia

Order: Cytophagales

Family: Cytophagaceae

Genus: Pontibacter

[Others may be used. Use NCBI link to find]

Species

NCBI: Taxonomy

Genus species Pontibacter Salisaro

Habitat Information

Latitude: 30.14402 degrees Longitude: -97.78506 degrees

It was a cloudy day high of 58 degrees low of 44 degrees on Jan 30th 2015. A spade and a glass mason jar to collet the soil sample. The soil was collected after digging 1 foot below the surface. The location of the soil sample chosen was in a natural ravine off the end of a gated home property Rainfall was 0.0" and the pressure was 28.53" The location was mostly Lewisville silty clay at 1-2 percent slopes.

Description and Significance

Describe the appearance (colonial and cellular), possible antimicrobial activity etc. of the organism, and why the organism might be significant.

Cellular: gram negative aerobic rod shaped red pigmented

Colonial: red pigmented round convex smooth

This soil microbe was placed on a lawn of Pseudomonas Aeruginosa, which is a gram negative bacteria, and it had a zone of inhibition which indicates antimicrobial capabilities.

Genome Structure

The Genome structure Pontibacter salisaro strain HMC5104 16S ribosomal RNA gene, partial sequence. It is linear, and not much else is known about this specific bacteria as of yet.

MR 19 forwards strain Max score: 907 total score: 907 Query cover: 85% Indetify: 98% accension: KC977017.1

MR 20 reverse Max score: 1282 Total score: 1282 Query Cover: 99% Indetify: 99% Accension: KC9677017.1

S Ribosomal sequence (partial):

                                1 ctataatgca gtcgaacgga ccttgagact tcggtttctt ggttagtggc gcacgggtgc
                                61 gtaacgcgta tgcaacctac cttccactgg gggatagccc ggggaaaccc ggattaatac
                                121 cgcatgacat cgtttgaggg catccgaaga cgatcaaagc tgaggcggtg gaagatgggc

Cell Structure, Metabolism and Life Cycle

Currently, Pontibacter Salisaro has not been observed for it's cell structure, metabolism and life cycle.

Here are the results from our lab tests.

It had a zone of inhibition against a lawn of Pseudamonas Aeruginosa

TSI: no fermentation however slight acidic

SIM: Positive for motility

Methyl Red: positive: acidic and is capable for mixed acid fermentation

Nitrate: positive change from Nitrate to Nitrite

Blood agar: alpha hemolysis which refers to partial hemolytic breakdown

PEA test: Gram negative bacteria because inhibition of growth

Bile Esculin and 6.5% salt tolerance test: tested positive for other group D enteroccoci

Physiology and Pathogenesis

Currently, Pontibacter Salisaro has not bee observed in relation to its pathogenic abilities, disinfectants and antibiotic tests were run on agar. It is resistant to oxacillian.

Lab notes

This bacteria was particularly difficult to isolate. I had multiple issues with contamination however upon further review of lab results and research lab book, the contamination could have resulted from not using aseptic techniques long enough in order to kill off any pre-existing bacteria on the loops used, possibly not keeping the covers of the plates and tubes used to culture the microbe closed and/or open for slanted and short periods of time as to not receive air contamination. When looking further into the bacteria there is still not enough available information in journals and books to cross reference information obtained in the lab. It seems this microbe was found in 2011 in South Korea and is still being typed and organized.

References

1. Joung, Y., Kim, H., Ahn, T.-S., and Joh, K. "Pontibacter salisaro sp. nov., isolated from a clay tablet solar saltern in Korea." J. Microbiol. (2011) 49:290-293.

Author

Page authored by Wendie Speed_, student of Prof. Kristine Hollingsworth at Austin Community College.