Methylosinus trichosporium: Difference between revisions

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Genus: Methylosinus
Genus: Methylosinus


[[File:M.trichosporium.png]
                                                                                                                                                  [[File:M.trichosporium.png]]
==Species==
==Species==
''Methylosinus trichosporium'' (strain ATCC35070)
''Methylosinus trichosporium'' (strain ATCC35070)
=Description and significance=
=Description and significance=
Methylosinus trichosporium is a gram negative, aerobic bacteria. This bacteria is found in the soil or in groundwater as well. Trichosporium is a bacteria that relies on methane as a source of carbon and energy. This bacteria not only helps by consuming and getting rid of methane it also serves another purpose as it has shown the ability to degrades Trichloroethylene and cometabolism of polyhydroxybutyrate. Trichloroethylene a chemical that is toxic to aquatic life, plants and animals.
''Methylosinus trichosporium'' is a Gram-negative, aerobic bacterium that relies on methane as a source of carbon and energy. This bacterium not only helps by consuming and getting rid of methane but also serves another purpose as it has shown the ability to degrade trichloroethylene and cometabolism of polyhydroxybutyrate. Trichloroethylene is a chemical that is toxic to aquatic life, plants, and animals. (''Methylosinus trichosporium''. Wikipedia the free encyclopedia https://en.wikipedia.org/wiki/Methylosinus_trichosporium)


=16S Ribosomal RNA Gene Information=
=16S Ribosomal RNA Gene Information=
Methylosinus trichosporium is a species of a-proteobacteria in the family Methylocystaceae. ("Methylosinus trichosporium". national library of medicine https://www.ncbi.nlm.nih.gov/data-hub/taxonomy/426/)
                                                                                                                                                                            [[File:Phylogenetic-Analysis-of-the-16S-rDNA-from-Methylosinus-trichosporium-IMV-3011-The-tree.png]].   
=Genome structure=
The genome structure methylosinus trichosporium was  sequenced, assembled, and annotated by the U.S. Department of Energy Joint Genome Institut. 44 contigs and 9 scaffolds was assembled from Roche 454-FLX, 454-std, 454-Titanium PE, and Illumina Solexa reads using Velvet (21). Automatic annotation was done using the Prokaryotic Dynamic Programming Genefinding Algorithm. Genome is 4.9 Mbp in size, contains 66% G+C, and encodes a single rRNA operon (Vallenet, D., S. Engelen, D. Mornico, S. Cruveiller, L. Fleury, A. Lajus, Z. Rouy, D. Roche, G. Salvignol, C. Scarpelli, and C. Medigue et al., 2009)


=Genome Structure (if the genome exists)=
The 16S rRNA sequencing of
The genome structure methylosinus trichosporium was  sequenced, assembled, and annotated by the U.S. Department of Energy Joint Genome Institut. 44 contigs and 9 scaffolds was assembled from Roche 454-FLX, 454-std, 454-Titanium PE, and Illumina Solexa reads using Velvet (21). Automatic annotation was done using the Prokaryotic Dynamic Programming Genefinding Algorithm. Genome is 4.9 Mbp in size, contains 66% G+C, and encodes a single rRNA operon
=Ecology and Pathogenesis=
=Ecology and Pathogenesis=
"Methylosinus trichosporium" has been found in various areas such as Soil, fresh water sediments, groundwater. )Whittenbury et al., 1970b) (Bowman et al., 1993) (Stein et al., 2010). "Methylosinus trichosporium" is mentioned to be non pathogenic to animals or humans according to a couple of books and articles including Bergey's Manual of Systematic Bacteriology and "fems microbiology  ecology.
                                            [[File:Screen Shot 2023-04-28 at 10.19.42 PM.png|400px|thumb|right|alt text]]


=Current Research=
=Current Research=
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https://pubmed.ncbi.nlm.nih.gov/29909832/, Soo Y, and Amy C Rosenzweig. “Recent Advances in the Genetic Manipulation of Methylosinus trichosporium OB3b.” Methods in enzymology vol. 605 (2018): 335-349. doi:10.1016/bs.mie.2018.02.011
https://pubmed.ncbi.nlm.nih.gov/29909832/, Soo Y, and Amy C Rosenzweig. “Recent Advances in the Genetic Manipulation of Methylosinus trichosporium OB3b.” Methods in enzymology vol. 605 (2018): 335-349. doi:10.1016/bs.mie.2018.02.011
Stein, L., Yoon, S., Semrau, J. D., Dispirito, A. A., Crombie, A., Murrell, J. C., Vauilleumier, S., Kalyuzhnaya, M. G., OP den camp, H. J., Bringer, G., D.Bruce, Cheng, J.-F., Copeland, A., Goodwin, L., Han, S., Hauser, L., Jetten, M. S. m., Lajus, A., Land, M. L., … Klotz, M. G. (2010, December 15). Journal of bacteriology. Journal of Bacteriology. Retrieved April 28, 2023, from https://journals.asm.org/doi/10.1128/JB.00144-06
Vallenet, D., S. Engelen, D. Mornico, S. Cruveiller, L. Fleury, A. Lajus, Z. Rouy, D. Roche, G. Salvignol, C. Scarpelli, and C. Medigue. 25 November 2009. MicroScope: a platform for microbial genome annotation and comparative genomics. Database (Oxford) 2009 :bap021.
https://www.ncbi.nlm.nih.gov/data-hub/taxonomy/426/
=author=
Page created by Erik Contreras, a student of Dr. Hidetoshi Urakawa of Florida Gulf Coast University for Microbial Ecology EVR 4024C.

Latest revision as of 04:40, 4 May 2023

Classification

Domain:Bacteria

Phylum: Pseudomonadota

Class: Alphaproteobacteria

Order: Hyphomicrobiales

Family: Methylocystaceae

Genus: Methylosinus 

                                                                                                                                                  M.trichosporium.png

Species

Methylosinus trichosporium (strain ATCC35070)

Description and significance

Methylosinus trichosporium is a Gram-negative, aerobic bacterium that relies on methane as a source of carbon and energy. This bacterium not only helps by consuming and getting rid of methane but also serves another purpose as it has shown the ability to degrade trichloroethylene and cometabolism of polyhydroxybutyrate. Trichloroethylene is a chemical that is toxic to aquatic life, plants, and animals. (Methylosinus trichosporium. Wikipedia the free encyclopedia https://en.wikipedia.org/wiki/Methylosinus_trichosporium)

16S Ribosomal RNA Gene Information

Methylosinus trichosporium is a species of a-proteobacteria in the family Methylocystaceae. ("Methylosinus trichosporium". national library of medicine https://www.ncbi.nlm.nih.gov/data-hub/taxonomy/426/) 

                                                                                                                                                                           Phylogenetic-Analysis-of-the-16S-rDNA-from-Methylosinus-trichosporium-IMV-3011-The-tree.png.


Genome structure

The genome structure methylosinus trichosporium was sequenced, assembled, and annotated by the U.S. Department of Energy Joint Genome Institut. 44 contigs and 9 scaffolds was assembled from Roche 454-FLX, 454-std, 454-Titanium PE, and Illumina Solexa reads using Velvet (21). Automatic annotation was done using the Prokaryotic Dynamic Programming Genefinding Algorithm. Genome is 4.9 Mbp in size, contains 66% G+C, and encodes a single rRNA operon (Vallenet, D., S. Engelen, D. Mornico, S. Cruveiller, L. Fleury, A. Lajus, Z. Rouy, D. Roche, G. Salvignol, C. Scarpelli, and C. Medigue et al., 2009)

Ecology and Pathogenesis

"Methylosinus trichosporium" has been found in various areas such as Soil, fresh water sediments, groundwater. )Whittenbury et al., 1970b) (Bowman et al., 1993) (Stein et al., 2010). "Methylosinus trichosporium" is mentioned to be non pathogenic to animals or humans according to a couple of books and articles including Bergey's Manual of Systematic Bacteriology and "fems microbiology ecology.

alt text

Current Research

Current research is underway for "Methylosinus trichosporium" it is currently being studied for multiple things such as methane assimilation, copper accumulation, and metal-dependent gene expression. Recent interest in methanotroph metabolic engineering has led to new protocols for genetic manipulation of methanotrophs that are effective and simple to use. "Methylosinus trichosporium" has shown to express both sMMO and pMMO, methanobactin (Dassama et al.,2017) and can express two distinct MDHs, the calcium-binding MxaF and the lanthanide-binding XoxF (Haque. Kalidass, et al., 2015).


References

https://www.frontiersin.org/articles/10.3389/fmicb.2013.00040/full#h10=Author= 

https://en.wikipedia.org/wiki/Methylosinus_trichosporium#:~:text=Methylosinus%20trichosporium%20is%20an%20obligate,ability%20to%20oxidize%20methane%2C%20M.

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6010078/, Soo Y, and Amy C Rosenzweig. “Recent Advances in the Genetic Manipulation of Methylosinus trichosporium OB3b.” Methods in enzymology vol. 605 (2018): 335-349. doi:10.1016/bs.mie.2018.02.011

https://pubmed.ncbi.nlm.nih.gov/29909832/, Soo Y, and Amy C Rosenzweig. “Recent Advances in the Genetic Manipulation of Methylosinus trichosporium OB3b.” Methods in enzymology vol. 605 (2018): 335-349. doi:10.1016/bs.mie.2018.02.011

Stein, L., Yoon, S., Semrau, J. D., Dispirito, A. A., Crombie, A., Murrell, J. C., Vauilleumier, S., Kalyuzhnaya, M. G., OP den camp, H. J., Bringer, G., D.Bruce, Cheng, J.-F., Copeland, A., Goodwin, L., Han, S., Hauser, L., Jetten, M. S. m., Lajus, A., Land, M. L., … Klotz, M. G. (2010, December 15). Journal of bacteriology. Journal of Bacteriology. Retrieved April 28, 2023, from https://journals.asm.org/doi/10.1128/JB.00144-06

Vallenet, D., S. Engelen, D. Mornico, S. Cruveiller, L. Fleury, A. Lajus, Z. Rouy, D. Roche, G. Salvignol, C. Scarpelli, and C. Medigue. 25 November 2009. MicroScope: a platform for microbial genome annotation and comparative genomics. Database (Oxford) 2009 :bap021.

https://www.ncbi.nlm.nih.gov/data-hub/taxonomy/426/

author

Page created by Erik Contreras, a student of Dr. Hidetoshi Urakawa of Florida Gulf Coast University for Microbial Ecology EVR 4024C.

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