Gisella Alcaraz-Bacillus Pumilus
==Classification== NCBI link to find]
Domain: Kingdom: Phylum: Class: Order: Family: Genus:
Species
NCBI: Taxonomy |
Genus species
Habitat Information
Describe the location and conditions under which the organism was isolated.
Description and Significance
Describe the appearance (colonial and cellular), possible antimicrobial activity etc. of the organism, and why the organism might be significant.
Colony Morphology:
Cellular Morpholoy:
When spread on a plate of Gram (+) E. faecalis, it produced a slight zone of inhibition.
Genome Structure
Describe the size and content of the genome. How many chromosomes? Circular or linear? Other interesting features? What is known about its sequence? Include S Ribosomal sequence that you obtained from PCR and sequencing here.
Cell Structure, Metabolism and Life Cycle
Interesting features of cell structure; how it gains energy; what important molecules it produces.
Physiology and Pathogenesis
Biochemical characteristics, enzymes made, other characteristics that may be used to identify the organism; contributions to environment (if any).
If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.
Further testing and Results from Biochemical Tests:
MR-VP (Methyl Red and Voges-Proskauer)Tests:
- No mixed acid fermentation
- Neutral end products
Citrate Test:
- Organism can use Citrate as the only carbon source.
SIM Test:
- Cannot use Sulfur as terminal electron acceptor
- Organism is not motile
- Tryptophan is not broken down and indole is not produced.
Nitrate Test:
- Organism did not make NO2 from NO3 OR organism broke down NO3 into another nitrogenous product.
- After adding zinc solution turned red = Organism did not reduce nitrate.
Urea Test:
- Organism is not able to break down Urea to Ammonia
TSI Test:
- Red Slant/ Yellow Butt/No gas production ___________________________________________________________________
Decarboxylation Test:
- Arginine-
- Lysine-
- Ornithine-
Phenylaline Deaminase Test:
Oxidase Test : (-) ______________________________________________________________________________
Eosin Methyline Blue Agar (EMB):
Hektoen Enteric Agar (HE):
MacConkeyh Agar (MAC):
Blood Agar Test:
Mannitol Salt Agar (MSA):
Phenylethyl Alcohol Agar (PEA):
Catalase Test :
6.5% Salt Tolerance Test:
Bile Esculin Test:
References
Author
Page authored by Gisella Alcaraz, student of Prof. Kristine Hollingsworth at Austin Community College.