Acid mine drainage

From MicrobeWiki, the student-edited microbiology resource

Introduction

Acid mine drainage in a stream just outside of Pittsburgh, PA. [[1]]

Acid mine drainage (AMD) occurs when metal sulfides, most commonly pyrite, are exposed to and react with air and water. When water flows over or through sulfur-bearing mine tailings, a chemical reaction occurs between the water and rocks resulting in metal-rich water. AMD typically has a bright orange, yellow, or bownish-red color to it. The mine drainage is acidic, as its name states, and found around ore and coal mines. Abandoned and currently operating mining activities contribute to AMD. Metal rich drainage can also occur in areas that have not been mined. Areas like construction sites and other places that have been highly disturbed can exacerbate natural rock weathering processes leading to acidic drainage.

An area heavily mined for coal, therefore greatly impacted by AMD, is the mid-Atlantic region in the middle of the Appalachian Mountains. Here, and everywhere AMD occurs, has a highly altered ecosystem. The greatest consequence of AMD is water pollution. This in turn results in contaminated drinking water, damage to aquatic flora and fauna, and corrosion of man-made infrastructure.

Microorganisms like bacteria and archaea significantly affect AMD. When metal sulfides, usually pyrite, that are contained in rock are exposed to water and air, an oxidation reaction takes place. Microbes speed up the decomposition of these metal ions. Microorganisms also play a huge part in the bioremediation of AMD. Techniques that are being researched include using metal-immobilizing bacteria, biocontrol with bacteria and archaea, and bioleaching. [3]

Physical environment

Physical & Chemical Characteristics

AMD is effected by characteristics such as pore size, particle size, and mineral composition of the materials being oxidized. Water and oxygen availability are the most important factors though.

Oxidation of Pyrite

A lot of mines use the technique of sub-surface mining, where extraction occurs beneath the earth’s surface. With this technique mines are commonly below the water table, requiring water to be pumped out of the mine to stop flooding. When a mine is deserted, water once again fills in its natural position beneath the surface. This water is one of the necessary ingredients for the oxidation of pyrite leading to AMD formation.

Exposing pyrite to oxygen and water leads to an oxidation reaction, where hydrogen and sulfate ions and soluble metal cations are created:

2FeS2(s) + 7O2(g) + 2H2O(l) → 2Fe2+(aq) + 4SO42-(aq) + 4H+(aq)

Pyrite oxidation occurs naturally at a slow rate in undisturbed rock. However, the acidity created is buffered by water. Because mining exposes more surface area of these sulfur-bearing rocks, additional acid is produced that is beyond the water’s usual buffering capabilities.

When enough oxygen is available either from dissolved oxygen in the water or the atmosphere, further oxidation of ferrous iron (Fe+2) to ferric iron (Fe+3) occurs:

4Fe2+(aq) + O2(g) + 4H+(aq) → 4Fe3+(aq) + 2H2O(l)

Ferric iron (Fe+3) can either precipitate as ochre (Fe(OH)3 ), the reddish-orange precipitate observed in acid mine drainage waters:

2Fe3+(aq) + 6H2O(l) <→ 2Fe(OH)3¬(s) + 6H+(aq)

or it can react directly with pyrite to make additional ferrous iron and hydrogen ions:

FeS2(s) + 14Fe3+(aq) + 8H2O(l) → 15Fe2+(aq) + 2SO42-(aq) + 16H+(aq)

Overall, these reactions release hydrogen ions, which decreases pH leading to an acidic environment. [4]

Metal Contamination

AMD is also known to be contaminated by toxic metals such as copper and nickel and lesser trace metals like lead, aluminum, arsenic, and manganese. Heavy metals are normally less toxic at pH levels near neutral. On the other hand, trace metals like cadmium, copper, and zinc which may also be found in mine drainage, are toxic at even the lowest concentrations [5].

Effects on Environment

AMD effects' on the environment vary from the eradication of all but a few most tolerant algae, macroinvertebrates, and fish, to insignificant or no effect. According to Jane Earle and Thomas Callaghan of the Pennsylvania Department of Environmental Protection, "the most severe effects are caused by high volume, low pH discharges with high concentrations of dissolved metals that drain into lightly buffered streams and produce accumulations of precipitated iron or aluminum. Little or no effect may occur from low volume or alkaline discharges with relatively low concentrations of metals that drain into moderate or highly buffered streams." [6]

Role of Microbes

Catalyze Oxidation

The general agreement among scientists of microbial-induced pyrite oxidation is that these microorganisms raising the amount of available ferric iron which increases the rate of pyrite oxidation. Acidithiobacillus ferrooxidans, for example, use reduced ferrous iron in AMD areas as an electron donor for energy creation at low pH.

Treatment Methods

  • Bioleaching: the process of using microorganisms to extract certain metals from low-grade ores. Sulfate-reducing bacteria use sulfate rather than oxygen to oxidize organic matter. Their metabolic products include bicarbonate, a water neutralizing substance, and hydrogen sulfide, which develops highly insoluble precipitates with a number of toxic metals. [7].
  • Reactive Permeable Barriers: barriers made of organic matter that are placed in the path of groundwater flow that react with specific substances of concern while allowing water to flow through easily. The barriers can be composed of materials like leaf compost, municipal compost, woodchips, or sawdust. The organic matter encourages the growth of sulfate-reducing bacteria which reduce sulfate to sulfide, resulting in the subsequent formation of insoluble metal sulfides. In the process, pH is increased therefore making the environment less acidic [8].
  • Bactericides: To stop the oxidation of Fe+2 to Fe+3, anionic surfactants are applied on surfaces where sulfide oxidizing bacteria are likely to inhabit. Bactericides thereby control pyrite oxidation. Commonly used in situations where the control of AMD formation is urgent, bactericides work best on fresh, unoxidized sulfides. They usually are produced in liquid solutions so application to mining equipment, infrastructure, and acid-producing substances. Bactericides can save mining companies thousands of dollars in treatment costs. Seen as a promising treatment method, they seem to work best along with other control methods. [9]

Key Microorganisms

Named extremophiles because of their ability to live in severe conditions, microbes living in AMD have to deal with low pH levels (extreme acidophiles) and sometimes high temperatures (extreme thermophiles). There are more microorganisms besides archaea and bacteria present in AMD. Other potential individuals in the community include fungi, algae, protozoa, and yeasts [10].

Bacteria

Scanning electron micrograph of A. ferrooxidans[[2]]
  • Acidithiobacillus ferrooxidans
  • Acidithiobacillus thiooxidans
  • Leptospirillum ferrooxidans
  • Sulfobacillus thermosulfidooxidans

Archaea

Ferroplasma acidiphilum

Adaptations

Organisms living in such harsh conditions must have adapted special systems for tolerating their environment. Scientists are particularly interested in the ability of microorganisms to to maintain internal pH homeostasis in such acidic conditions. The most studied acidophilic chemolithotroph is Acidithiobacillus ferrooxidans. A. ferrooxidans grows best in the range of pH from 1.5 to 3.5. But within this range, it is able to retain a cytoplasmic pH near neutral. Some research suggests a reversed membrane potential is responsible for internal pH maintenance, though the specific mechanisms for extreme acidophiles are unknown [11].

Current Research

Iron Mountain, California

At Iron Mountain near Redding, CA research is currently focusing on a “molecular-level understanding of the metabolism of organisms involved in AMD formation”. The project is using several methods to identify the molecular community and the roles of specific organisms present. DNA sequence analysis is used to learn what organisms are in the environment and then fluorescence in-situ hybridizations (FISH) determines cell type distribution and geochemical conditions. Samples are taking from different locations such as sediments, pore fluids, free-flowing waters, and subaerial biofilms. These field samples then serve as innoculum in various media that are incubated under aerobic, microaerophillic, and anaerobic conditions. Information about growth rates, metabolic capability, and optimal growth conditions is taken from isolates which are then identified through DNA sequence analyses. Currently, analysis has identified Leptospirillum group II, Leptospirillum group III, and Ferroplasma acidarmanus. [12]

Acid Drainage Technology Initiative, West Virginia

Begun in 1995 by Federal agencies, the Acid Drainage Technology Initiative (ADTI) was created “to identify, evaluate, and develop cost-effective and practical acid drainage technologies”. The main goal of ADTI is to address drainage quality concerns with metal mining and acid drainage from coal mines for “abandoned, active and future mines”. ADTI wants to develop a consensus among regulatory agencies (Federal and state) and industry through techonology development. Specific studies include in-situ deep mine technology demonstration where they are injecting alkaline solutions into the acidic mine pool. [13]

Silver Bow County, Montana

At the deserted Calliope mine, three Sulfate Reducing Bacteria bioreactors were installed to treat discharge waters. A bioreactor is a lined pit or trench that contains various substances, usually a combinations of compost, other organic matter, cobbles, and sometimes an alkaline agent, over which water flows. In this study, two of the three reactors were positioned below grade to limit the effects of temperature changes and one above ground to investigate the results of freezing. Filled with cobbles, crushed limestone and cobbles, the reactors were monitored monthly. The results were a reduction in acidity and toxic metal concentrations. Overall, the study was a success showing future potential for bioreactors as a treatment method. [14]

References

1. Curtis, Brandon. 2009. “Acid Mine Drainage, Wester Pa Style”. Flickr.

2. Murr, L. 2006. “Biological Issues in Materials Science and Engineering: Interdisciplinarity and the Bio-Materials Paradigm”. JOM 58:23-33.

3. “What is Acid Mine Drainage”. U.S. Environmental Protection Agency. http://www.sosbluewaters.org/epa-what-is-acid-mine-drainage%5B1%5D.pdf

4. Costello, C. 2003. “Acid Mine Drainage: Innovative Treatment Technologies”. National Network of Environmental Management Studies.

5.Earle, J. and Callaghan, T. 1998. Coal Mine Drainage Prediction and Pollution Prevention in Pennsylvania. Chapter 4: Impacts of mine Drainage on Aquatic Life, Water Uses, and Man-made Structures. p 1-10.

6. See reference 5.

7. See reference 4.

8. See reference 4.

9. Skousen, J., Sexstone, A., and Ziemkiewicz, P. 2000. “Reclamation of Drastically Distrubed Lands”. American Society of Agronomy and American Society for Surface Mining and Reclamation. Agronomy 41: 1-42.

10. Johnson, D.B. 1998. “Biodiversity and ecology of acidophilic microorganisms”. FEMS Microbiology Ecology 27: 307–317

11. Allen, E. and Banfield, J. 2005. “Community genomics in microbial ecology and evolution”. Nauture Reviews Microbiology 3:489-498.

12. See reference 11.

13. “Acid Drainage Technology Initiative. West Virginia Water Research Institute. West Virginia University. http://wvwri.nrcce.wvu.edu/programs/adti/index.cfm#listing

14. See reference 4.


Edited by student of Angela Kent at the University of Illinois at Urbana-Champaign.