Difference between revisions of "Aeropyrum pernix"

From MicrobeWiki, the student-edited microbiology resource
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==Genome structure==
 
==Genome structure==
Describe the size and content of the genome.  How many chromosomes? Circular or linear?  Other interesting features? What is known about its sequence?
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The genome is composed of 1,669,695 base pairs and contains 14 tRNA genes with introns, like the P. horikoshii’s genome (anaerobic archaen)It has a single circular chromosome. Some interesting features of this genome is that all the genes in the TCA cycle were found except for the alpha-ketoglutarate dehydrogenase gene and instead two sets of genes coding for the subunits of 2-oxoacid:ferredoxin oxidereductase were found in its place. One set is encoded in ORF’s ape2126 and ape2128 and the other in ape1473 and ape1472. These enzymes uses pyruvate as a main substrate.  Through sequence comparison among the assigned ORFs, most of the ORFs in the Aeropyrum pernix were generated by a sequence duplication.  Around 89.12% of the whole genome consisted of RNA coding regions and the assigned ORF.  The GC genome content is 67%
Does it have any plasmids?  Are they important to the organism's lifestyle?
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?list_uids=10382966&db=PubMed&dopt=Abstract&cmd=Retrieve]
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?list_uids=15848165&db=PubMed&dopt=Abstract&cmd=Retrieve]
  
 
==Cell structure and metabolism==
 
==Cell structure and metabolism==

Revision as of 18:47, 4 June 2007

A Microbial Biorealm page on the genus Aeropyrum pernix

Classification

Higher order taxa

Archaea; Crenarchaeota; Thermoprotei; Desulfurococcales; Desulfurococcaceae, Desulfurococcaceae; Aeropyrum [Others may be used. Use NCBI link to find]

Species

NCBI: Taxonomy

Aeropyrum pernix

Description and significance

The Aeropyrum pernix is an aerobic archaeon and is spherical in shape with a diameter of about 1 micro meters. It grows optimally at pH 7.0 and at a temperature of 95 degrees celcius and a salinity of 3.5%. This archaeon is important enough to have its genome sequenced because it can help answer how the Aeropyrum pernix is able to consume oxygen in such an extreme environment. Only anaerobic Archaea was found in such living conditions, until the discovery of the Aeropyrum pernix. Aeropyrum pernix was isolated at a hydrothermal vent at kodakara island in Kagoshima Prefecture by a team of researchers at Kyoto University in 1993. The whole genome was sequenced by using the shotgun sequencing approach. Include a picture or two (with sources) if you can find them.

Genome structure

The genome is composed of 1,669,695 base pairs and contains 14 tRNA genes with introns, like the P. horikoshii’s genome (anaerobic archaen). It has a single circular chromosome. Some interesting features of this genome is that all the genes in the TCA cycle were found except for the alpha-ketoglutarate dehydrogenase gene and instead two sets of genes coding for the subunits of 2-oxoacid:ferredoxin oxidereductase were found in its place. One set is encoded in ORF’s ape2126 and ape2128 and the other in ape1473 and ape1472. These enzymes uses pyruvate as a main substrate. Through sequence comparison among the assigned ORFs, most of the ORFs in the Aeropyrum pernix were generated by a sequence duplication. Around 89.12% of the whole genome consisted of RNA coding regions and the assigned ORF. The GC genome content is 67%

[1]

[2]

Cell structure and metabolism

Describe any interesting features and/or cell structures; how it gains energy; what important molecules it produces.

Ecology

Describe any interactions with other organisms (included eukaryotes), contributions to the environment, effect on environment, etc.

Pathology

How does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.

Application to Biotechnology

Does this organism produce any useful compounds or enzymes? What are they and how are they used?

Current Research

Enter summaries of the most recent research here--at least three required

References

[3] Complete genome sequence of an aerobic hyper-thermophilic crenarchaeon, Aeropyrum pernix K1.

[4] Gene expression and characterization of two 2-oxoacid:ferredoxin oxidoreductases from Aeropyrum pernix K1.

[5] Identification of the first archaeal oligopeptide-binding protein from the hyperthermophile Aeropyrum pernix.

[6] Proteome analysis of an aerobic hyperthermophilic crenarchaeon, Aeropyrum pernix K1.

[7] The acylaminoacyl peptidase from Aeropyrum pernix K1 thought to be an exopeptidase displays endopeptidase activity.

[8] Properties of the alpha subunit of a Chaperonin from the hyperthermophilic Crenarchaeon Aeropyrum pernix K1.


Edited by Daniel Pak, student of Rachel Larsen and Kit Pogliano