https://microbewiki.kenyon.edu/index.php?title=Amphibacillus_fermentum&feed=atom&action=historyAmphibacillus fermentum - Revision history2024-03-29T10:30:21ZRevision history for this page on the wikiMediaWiki 1.39.6https://microbewiki.kenyon.edu/index.php?title=Amphibacillus_fermentum&diff=79072&oldid=prevWilhelmR at 04:34, 27 December 20122012-12-27T04:34:53Z<p></p>
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 04:34, 27 December 2012</td>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>[[File:Afermentumnutrients2.png|<del style="font-weight: bold; text-decoration: none;">200px</del>|thumb|left|FIGURE 1. Energy sources utilized by ''A. fermentum'' under anaerobic and aerobic conditions.]]</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>[[File:Afermentumnutrients2.png|<ins style="font-weight: bold; text-decoration: none;">400px</ins>|thumb|left|FIGURE 1. Energy sources utilized by ''A. fermentum'' under anaerobic and aerobic conditions.]]</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>''A. fermentum'' are facultatively anaerobic chemoorganoheterotrophs [[#References|[1]]]. Under anaerobic conditions, they ferment glucose and other sugars to formate, acetate and ethanol [[#References|[1]]]. Formate production under anaerobic conditions is catalyzed by the enzyme pyruvate formate lyase, which cleaves pyruvate into formate [[#References|[8]]]. ''A. fermentum'' can oxidize additional sugars under aerobic conditions such as ribose, lactose and N-acetylglucosamine [[#References|[1]]]. Glucose metabolisation under aerobic conditions produces acetate as the major byproduct [[#References|[7]]]. ''A. fermentum'' exhibit high growth rates even in high mineral levels [[#References|[1]]]. In addition, ''A. fermentum'' cannot fix nitrogen though they can reduce sulfur, but it is not coupled with energy conservation [[#References|[1]]]. They are tolerant to sulfur and can grow in the presence of active sulfidogenesis [[#References|[1]]].</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>''A. fermentum'' are facultatively anaerobic chemoorganoheterotrophs [[#References|[1]]]. Under anaerobic conditions, they ferment glucose and other sugars to formate, acetate and ethanol [[#References|[1]]]. Formate production under anaerobic conditions is catalyzed by the enzyme pyruvate formate lyase, which cleaves pyruvate into formate [[#References|[8]]]. ''A. fermentum'' can oxidize additional sugars under aerobic conditions such as ribose, lactose and N-acetylglucosamine [[#References|[1]]]. Glucose metabolisation under aerobic conditions produces acetate as the major byproduct [[#References|[7]]]. ''A. fermentum'' exhibit high growth rates even in high mineral levels [[#References|[1]]]. In addition, ''A. fermentum'' cannot fix nitrogen though they can reduce sulfur, but it is not coupled with energy conservation [[#References|[1]]]. They are tolerant to sulfur and can grow in the presence of active sulfidogenesis [[#References|[1]]].</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>[[File:AfermentumNADH.png|<del style="font-weight: bold; text-decoration: none;">200px</del>|thumb|right|FIGURE 2. NADH re-oxidation by ''A. fermentum'' under anaerobic and aerobic conditions.]]</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>[[File:AfermentumNADH.png|<ins style="font-weight: bold; text-decoration: none;">400px</ins>|thumb|right|FIGURE 2. NADH re-oxidation by ''A. fermentum'' under anaerobic and aerobic conditions.]]</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>''A. fermentum'' can grow under both aerobic and anaerobic conditions because they possess both aerobic and anaerobic pathways to oxidize nutrients [[#References|[1]]]. In addition to the two major aerobic and anaerobic metabolic pathways exhibited in the type species ''A. xylanus'' [[#References|[6]]], ''A. fermentum'' possess a third metabolic pathway which produces lactate in both aerobic and anaerobic conditions [[#References|[7]]]. The ability to use three different metabolic pathways has allowed the organism to metabolize a large range of substrates [[#References|[8]]]. The NADH formed under anaerobic glycolysis is recycled to NAD<font size="-1"><sup>+</sup></font> by NAD- linked aldehyde dehydrogenase and NAD-linked alcohol dehydrogenase [[#References|[7]]]. While the NADH formed by the pyruvate pathway and glycolysis under the presence of oxygen can be re-oxidized by NADH oxidase [[#References|[7]]]. The contribution of each pathway to the overall metabolism depends on the growth phase of the organism and environmental factors such as temperature and nutrient levels [[#References|[8]]]. ''A. fermentum'' can effectively recycle NADH under both aerobic and anaerobic conditions, which allows them to be able to grow in both conditions [[#References|[7]]]. </div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>''A. fermentum'' can grow under both aerobic and anaerobic conditions because they possess both aerobic and anaerobic pathways to oxidize nutrients [[#References|[1]]]. In addition to the two major aerobic and anaerobic metabolic pathways exhibited in the type species ''A. xylanus'' [[#References|[6]]], ''A. fermentum'' possess a third metabolic pathway which produces lactate in both aerobic and anaerobic conditions [[#References|[7]]]. The ability to use three different metabolic pathways has allowed the organism to metabolize a large range of substrates [[#References|[8]]]. The NADH formed under anaerobic glycolysis is recycled to NAD<font size="-1"><sup>+</sup></font> by NAD- linked aldehyde dehydrogenase and NAD-linked alcohol dehydrogenase [[#References|[7]]]. While the NADH formed by the pyruvate pathway and glycolysis under the presence of oxygen can be re-oxidized by NADH oxidase [[#References|[7]]]. The contribution of each pathway to the overall metabolism depends on the growth phase of the organism and environmental factors such as temperature and nutrient levels [[#References|[8]]]. ''A. fermentum'' can effectively recycle NADH under both aerobic and anaerobic conditions, which allows them to be able to grow in both conditions [[#References|[7]]]. </div></td></tr>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>[[File:Lake_Magadi.jpeg|<del style="font-weight: bold; text-decoration: none;">200px</del>|thumb|right|FIGURE 3. Lake Magadi http://www.wildencounters.net/weblog/wp-content/uploads/2011/02/Lesser-Flamingos-on-the-edge-of-Lake-Magadi-Kenya-aerial-shot1.jpg]]</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>[[File:Lake_Magadi.jpeg|<ins style="font-weight: bold; text-decoration: none;">400px</ins>|thumb|right|FIGURE 3. Lake Magadi http://www.wildencounters.net/weblog/wp-content/uploads/2011/02/Lesser-Flamingos-on-the-edge-of-Lake-Magadi-Kenya-aerial-shot1.jpg]]</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br/></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>''A. fermentum'' was first isolated under anaerobic conditions from Lake Magadi, in Kenya [[#References|[1]]]. The isolates required yeast extract to provide methionine and grew on sucrose in single, pairs or in short chains [[#References|[1]]]. They are able to compete with obligate anaerobes under anaerobic conditions [[#References|[1]]]. In fact they should have a greater competitive ability since they are resistant to drying [[#References|[1]]]. ''A. fermentum'' interact with secondary anaerobes that utilize the formate, acetate, ethanol, and CO<font size="-1"><sub>2</sub></font> produced [[#References|[8]]]. In particular, the formate can be used for interspecies [http://en.wikipedia.org/wiki/Hydrogen_cycle hydrogen transfer] [[#References|[8]]]. The formate provides H<font size="-1"><sub>2</sub></font> for methanogens to synthesize methane via [http://en.wikipedia.org/wiki/Methanogenesis methanogenesis] [[#References|[9]]]. The consumption of H<font size="-1"><sub>2</sub></font> by methanogens is important for the growth of ''A. fermentum'' as anaerobic fermentation is inhibited by high H<font size="-1"><sub>2</sub></font> concentrations [[#References|[9]]]. </div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>''A. fermentum'' was first isolated under anaerobic conditions from Lake Magadi, in Kenya [[#References|[1]]]. The isolates required yeast extract to provide methionine and grew on sucrose in single, pairs or in short chains [[#References|[1]]]. They are able to compete with obligate anaerobes under anaerobic conditions [[#References|[1]]]. In fact they should have a greater competitive ability since they are resistant to drying [[#References|[1]]]. ''A. fermentum'' interact with secondary anaerobes that utilize the formate, acetate, ethanol, and CO<font size="-1"><sub>2</sub></font> produced [[#References|[8]]]. In particular, the formate can be used for interspecies [http://en.wikipedia.org/wiki/Hydrogen_cycle hydrogen transfer] [[#References|[8]]]. The formate provides H<font size="-1"><sub>2</sub></font> for methanogens to synthesize methane via [http://en.wikipedia.org/wiki/Methanogenesis methanogenesis] [[#References|[9]]]. The consumption of H<font size="-1"><sub>2</sub></font> by methanogens is important for the growth of ''A. fermentum'' as anaerobic fermentation is inhibited by high H<font size="-1"><sub>2</sub></font> concentrations [[#References|[9]]]. </div></td></tr>
</table>WilhelmRhttps://microbewiki.kenyon.edu/index.php?title=Amphibacillus_fermentum&diff=76841&oldid=prevAl201: Amphibacillus fermentum2012-12-12T06:24:16Z<p>Amphibacillus fermentum</p>
<p><b>New page</b></p><div>{{Uncurated}}<br />
=Classification=<br />
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Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Amphibacillus <br />
<br />
Species: ''Amphibacillus fermentum''<br />
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NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=173041&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy]<br />
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=Description=<br />
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''Amphibacillus fermentum'' are motile, gram positive, rod shaped, alkaliphilic, facultative anaerobes that are resistant to heat and dessication1. They were first isolated from the anaerobic regions of the alkaline [http://en.wikipedia.org/wiki/Lake_magadi Lake Magadi] in Kenya [[#References|[1]]]. ''A. fermentum'' requires Na+ and CO3- for growth and can grow at a pH of 7.0 to 10.5, though growth is optimized at a pH of 8.0 to 9.5 at 37 to 38<font size="-1"><sup>o</sup></font>C [[#References|[1]]]. They are saccharolytic chemoorganotrophs, which ferment glucose to formate, as well as ethanol and acetate under anaerobic conditions [[#References|[1]]]. Unlike other bacilli, ''A. fermentum'' do not form spores [[#References|[1]]]. However, as the bacteria grow independent of chloride levels [[#References|[1]]], they are resistant to the changing mineral and moisture levels of the alkaline environment and are highly adapted to their natural environment. Before the isolation of ''A. fermentum'', all bacilli isolated from alkaline lakes have been obligate aerobes [[#References|[2]]]. As such, ''A. fermentum'' serves as representatives of the anaerobic environment in alkaline lakes that undergo cycles of complete dessication [[#References|[1]]].<br />
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=Cell Structure and Life Cycle=<br />
<br />
''A. fermentum'' are thin rods with a subterminal flagella protruding from their gram positive cell wall [[#References|[1]]]. They divide by [http://en.wikipedia.org/wiki/Binary_fission binary fission], which can sometimes be unsymmetrical [[#References|[1]]]. ''A. fermentum'' are resistant to heat, and can resume growth after being heated to 80<font size="-1"><sup>o</sup></font>C [[#References|[1]]]. This is in contrast with other ''Amphibacillus'' species that produce spores in response to unfavorable heating [[#References|[1,3-6]]]. They are also resistant to drying, though unlike ''Amphibacillus tropicus'', they are not resistant when placed into salt crystals formed by evaporation of minerals [[#References|[1]]]. However, the natural environment of ''A. fermentum'' is not on salt crystals, therefore this does not necessarily represent the bacterium’s competitive ability to grow.<br />
<br />
The genome of ''A. fermentum'' has yet to be sequenced, however, the chemical characterization of the genome corroborates their classification as low G+C content (41.5%) member of the phylum, Firmicutes [[#References|[7]]]. The 16S rRNA gene of ''A. fermentum'' demonstrates the closest ancestry to the [http://en.wikipedia.org/wiki/Halotolerant haltolerant] ''Gracilibacillus halotolerans'', as opposed to the alkaliphilic ''Amphibacillus xylanus'' [[#References|[1]]]. It has been proposed that organisms unique to the alkaline environment have evolved separately [[#References|[2]]], which may explain the low homology between ''A. fermentum'' and ''A. xylanus''.<br />
<br />
=Metabolism=<br />
<br />
[[File:Afermentumnutrients2.png|200px|thumb|left|FIGURE 1. Energy sources utilized by ''A. fermentum'' under anaerobic and aerobic conditions.]]<br />
<br />
''A. fermentum'' are facultatively anaerobic chemoorganoheterotrophs [[#References|[1]]]. Under anaerobic conditions, they ferment glucose and other sugars to formate, acetate and ethanol [[#References|[1]]]. Formate production under anaerobic conditions is catalyzed by the enzyme pyruvate formate lyase, which cleaves pyruvate into formate [[#References|[8]]]. ''A. fermentum'' can oxidize additional sugars under aerobic conditions such as ribose, lactose and N-acetylglucosamine [[#References|[1]]]. Glucose metabolisation under aerobic conditions produces acetate as the major byproduct [[#References|[7]]]. ''A. fermentum'' exhibit high growth rates even in high mineral levels [[#References|[1]]]. In addition, ''A. fermentum'' cannot fix nitrogen though they can reduce sulfur, but it is not coupled with energy conservation [[#References|[1]]]. They are tolerant to sulfur and can grow in the presence of active sulfidogenesis [[#References|[1]]].<br />
<br />
[[File:AfermentumNADH.png|200px|thumb|right|FIGURE 2. NADH re-oxidation by ''A. fermentum'' under anaerobic and aerobic conditions.]]<br />
<br />
''A. fermentum'' can grow under both aerobic and anaerobic conditions because they possess both aerobic and anaerobic pathways to oxidize nutrients [[#References|[1]]]. In addition to the two major aerobic and anaerobic metabolic pathways exhibited in the type species ''A. xylanus'' [[#References|[6]]], ''A. fermentum'' possess a third metabolic pathway which produces lactate in both aerobic and anaerobic conditions [[#References|[7]]]. The ability to use three different metabolic pathways has allowed the organism to metabolize a large range of substrates [[#References|[8]]]. The NADH formed under anaerobic glycolysis is recycled to NAD<font size="-1"><sup>+</sup></font> by NAD- linked aldehyde dehydrogenase and NAD-linked alcohol dehydrogenase [[#References|[7]]]. While the NADH formed by the pyruvate pathway and glycolysis under the presence of oxygen can be re-oxidized by NADH oxidase [[#References|[7]]]. The contribution of each pathway to the overall metabolism depends on the growth phase of the organism and environmental factors such as temperature and nutrient levels [[#References|[8]]]. ''A. fermentum'' can effectively recycle NADH under both aerobic and anaerobic conditions, which allows them to be able to grow in both conditions [[#References|[7]]]. <br />
<br />
=Ecology=<br />
<br />
[[File:Lake_Magadi.jpeg|200px|thumb|right|FIGURE 3. Lake Magadi http://www.wildencounters.net/weblog/wp-content/uploads/2011/02/Lesser-Flamingos-on-the-edge-of-Lake-Magadi-Kenya-aerial-shot1.jpg]]<br />
<br />
''A. fermentum'' was first isolated under anaerobic conditions from Lake Magadi, in Kenya [[#References|[1]]]. The isolates required yeast extract to provide methionine and grew on sucrose in single, pairs or in short chains [[#References|[1]]]. They are able to compete with obligate anaerobes under anaerobic conditions [[#References|[1]]]. In fact they should have a greater competitive ability since they are resistant to drying [[#References|[1]]]. ''A. fermentum'' interact with secondary anaerobes that utilize the formate, acetate, ethanol, and CO<font size="-1"><sub>2</sub></font> produced [[#References|[8]]]. In particular, the formate can be used for interspecies [http://en.wikipedia.org/wiki/Hydrogen_cycle hydrogen transfer] [[#References|[8]]]. The formate provides H<font size="-1"><sub>2</sub></font> for methanogens to synthesize methane via [http://en.wikipedia.org/wiki/Methanogenesis methanogenesis] [[#References|[9]]]. The consumption of H<font size="-1"><sub>2</sub></font> by methanogens is important for the growth of ''A. fermentum'' as anaerobic fermentation is inhibited by high H<font size="-1"><sub>2</sub></font> concentrations [[#References|[9]]]. <br />
<br />
Processes such as mining and food processing factories have expanded the number of alkaline environments present [[#References|[8]]]. Alkaline lakes are almost closed systems, with photosynthetic cyanobacteria providing biologically available carbon and nitrogen [[#References|[2]]]. ''A. fermentum'' play a role in cycling the influx of nutrients. Other organisms growing under the presence of oxygen can use the aerobically produced acetate [[#References|[2]]]. These aerobic organisms possess hydrolytic enzymes and can therefore metabolize a variety of nutrients, including the metabolites produced by ''A. fermentum'' [[#References|[2]]]. In anaerobic conditions, the ethanol and acetate byproducts can be used by haloalkaliphiles, but not by the obligate methylotrophs as they only use methanol as substrate [[#References|[2]]]. <br />
<br />
=Significance=<br />
<br />
General alkaliphiles do not produce neutralizing compounds, however ''A. fermentum'' does produce ethanol [[#References|[10,11]]]. ''Exiguobacterium'' sp. is alkaliphilic and can neutralize wastewater from a pH of 12 to a pH of 7.5 [[#References|[11]]]. ''A. fermentum'' can maintain an intracellular pH that is two units lower than the environmental pH, and as such, can maintain an internal pH that is optimum for their enzymes despite the alkaline external environment [[#References|[8]]]. As ''A. fermentum'' can also produce neutralizing metabolites [[#References|[8]]], they may be used in bioremediation of waste in alkaline conditions such as wastewater from the beverage industry [[#References|[11]]]. Glucose, sucrose and fructose present in the beverage waste can be used as nutrient sources for ''A. fermentum'' and under anaerobic conditions, formate and ethanol can be formed as byproduct which aids in reducing the environmental pH [[#References|[11]]]. The ability for ''A. fermentum'' to competitively compete and grow under aerobic and anaerobic conditions makes it a practical candidate in bioremediation of alkaliphilic environments. <br />
<br />
=References=<br />
<br />
(1) Zhilina, T.N., Garnova, E.S., Tourova, T.P., Kostrikina, N.A. and Zavarzin, G.A. “Amphibacillus fermentum sp. nov. and Amphibacillus tropicus sp. nov., New Alkaliphilic, Facultatively Anaerobic, Saccharolytic Bacilli from Lake Magadi.” MICROBIOLOGY, 2001, DOI: 10.1023/A:1013196017556<br />
<br />
(2) Jones, B.E., Grant, W.D., Duckworth, A.W., and Owenson, G.G. “Microbial diversity of soda lakes.” EXTREMOPHILES, 1998, DOI: 10.1007/s007920050060<br />
<br />
(3) Pugin, B., Blamey, J.M., Baxter, B.K., and Wiegel, J. “Amphibacillus cookii sp. nov., a facultatively aerobic, spore-forming, moderately halophilic, alkalithermotolerant bacterium.” International Journal of Systematic and Evolutionary Microbiology, 2012, DOI: 10.1099/ijs.0.034629-0<br />
<br />
(4) Wu, X.Y., Zheng, G., Zhang, W.W., Xu, X.W., Wu, M., and Zhu, X.F. “Amphibacillus jilinensis sp. nov., a facultatively anaerobic alkaliphilic bacillus from a soda lake.” International Journal of Systematic and Evolutionary Microbiology, 2010, DOI: 10.1099/ijs.0.018259-0<br />
<br />
(5) An, S.Y., Ishikawa, S., Kasai, H., Goto, K., and Yokota, A. “Amphibacillus sediminis sp. nov., an endospore-forming bacterium isolated from lake sediment in Japan.” International Journal of Systematic and Evolutionary Microbiology, 2009, DOI: 10.1099/ijs.0.64926-0<br />
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