Bacillus Pumilus RTMG
Classification
Domain: Bacteria
Phylum: Firmicutes
Class: Bacilli
Order: Bacillales
Family: Bacillaceae
Genus: Bacillus
Other Names:
Species
NCBI: Taxonomy |
Genus species: Bacillus pumilus
Habitat Information
Latitude: 30.398974 degrees Longitude: -97.704909 degrees
It was a clear day with a temperature of 65 F in the area of central Austin on January 25th 2018. A zip lock bag was used to collect the soil from the area mostly from the surface about a half inch deep. The location of the soil sample was taken from an area close to residential buildings underneath a tree. Rainfall was 0.0" and the pressure was 30.13 inHg. The description of the location was mostly Austin silty clay and urban land. 2-5% slopes and eroded.
Description and Significance
Describe the appearance (colonial and cellular), possible antimicrobial activity etc. of the organism, and why the organism might be significant.
Cellular: gram negative aerobic
Colonial:
Genome Structure
Describe the size and content of the genome. How many chromosomes? Circular or linear? Other interesting features? What is known about its sequence? Include S Ribosomal sequence that you obtained from PCR and sequencing here.
ED2-Reverse_B05.ab1 ACCGTCCTCCCGAAGGTTAGACTAGCTACTTCTGGTGCAACCCACTCCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGA ACGTATTCACCGCGACATTCTGATTCGCGATTACTAGCGATTCCGACTTCACGCAGTCGAGTTGCAGACTGCGATCCGGACTACG ATCGGTTTTATGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTTTGTACCGACCATTGTAGCACGTGTGTAGCCCAGGCCGTA AGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCTTAGAGTGCCCACCATTACGTGCTG GTAACTAAGGACAAGGGTTGCGCTCGTTACGGGACTTAACCCAACATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTG TCTCAATGTTCCCGAAGGCACCAATCCATCTCTGGAAAGTTCATTGGATGTCAAGGCCTGGTAAGGTTCTTCGCGTTGCTTCGAA TTAAACCACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCATTTGAGTTTTAACCTTGCGGCCGTACTCCCCAGGCGGTCA ACTTAATGCGTTAGCTGCGCCACTAAGAGCTCAAGGCTCCCAACGGCTAGTTGACATCGTTTACGGCGTGGACTACCAGGGTAT CTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGGTGGTCGCCTTCGCCACTGGTGTTCCTTCCTA TATCTACGCATTTCACCGCTACACAGGAAATTCCACCACCCTCTACCATACTCTAGCTCGCCAGTTTTGGATGCAGTTCCCAGGT TGAGCCCGGGGATTTCACATCCAACTTAACGAACCACCTACGCGCGCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTC TGTATTACCGCG
Cell Structure, Metabolism and Life Cycle
Interesting features of cell structure; how it gains energy; what important molecules it produces.
Physiology and Pathogenesis
BIOCHEMICAL TEST RESULTS
- Phenol Red Broth Tests: Glucose: positive; Lactose: negative; Sucrose: negative
- Starch Hydrolysis Test: negative
- Casein Hydrolysis Test: negative
- Gelatin Hydrolysis Test: positive
- DNA Hydrolysis Test: slight positive
- Lipid Hydrolysis Test: positive
- Methyl Red Test: negative
- Voges Proskauer Test: negative
- Citrate Test: positive
- SIM Tests: negative for all
- Nitrate Reduction: negative
- Urea Hydrolysis: negative
- Triple Sugar Iron Agar: negative for all
- Oxidase Test: negative
- Eosin Methylene Blue Agar (EMB) Test: positive
- Hektoen Enteric Agar (HE) Test: negative
- MacConkey Agar Test: negative
- Decarboxylation Tests: Arginine: no change; Lysine: negative; Orinithine: negative
- Phenylalanine Deaminase Test: negative
- Catalase Test: negative
- Blood Agar Test: slight positive
- Mannitol Salt Agar (MSA) Test: negative
- Phenylethyl Alcohol Agar (PEA) Test: negative
- Bacitracin/Optochin Susceptibility Test: Bacitracin: resistant; Optochin: resistant
- Bile Esculin Test: negative
- 6.5% Salt Tolerance Test: slight positive
References
https://www.ncbi.nlm.nih.gov/pubmed/25410940, "Pseudomonas granadensis sp. nov., a new bacterial species isolated from the Tejeda, Almijara and Alhama Natural Park, Granada, Spain". PubMed.gov. Epub 2014 Nov 19.
Author
Page authored by Diane Marques and Emma Riegle, students of Prof. Kristine Hollingsworth at Austin Community College.