Bacillus Pumilus RTMG

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Classification

Domain: Bacteria

Phylum: Firmicutes

Class: Bacilli

Order: Bacillales

Family: Bacillaceae

Genus: Bacillus

Other Names:

Species

NCBI: Taxonomy

Genus species: Bacillus pumilus

Habitat Information

Latitude: 30.398974 degrees Longitude: -97.704909 degrees

It was a clear day with a temperature of 65 F in the area of central Austin on January 25th 2018. A zip lock bag was used to collect the soil from the area mostly from the surface about a half inch deep. The location of the soil sample was taken from an area close to residential buildings underneath a tree. Rainfall was 0.0" and the pressure was 30.13 inHg. The description of the location was mostly Austin silty clay and urban land. 2-5% slopes and eroded.

Description and Significance

Describe the appearance (colonial and cellular), possible antimicrobial activity etc. of the organism, and why the organism might be significant.


Cellular: Gram positive

Colonial: rod-shape

Aerobic

Spore-Forming

Genome Structure

Describe the size and content of the genome. How many chromosomes? Circular or linear? Other interesting features? What is known about its sequence? Include S Ribosomal sequence that you obtained from PCR and sequencing here.

>RM1_4-16S-rRNA-SeqF_D09.ab1

ATTATTGGGCGTANGGGCTCGCAGGCGGTTTCTTAAGTCTGATGTGAAAGCCCCCGGCTCAACC GGGGAGGGTCATTGGAAACTGGGAAACTTGAGTGCAGAAGAGGAGAGTGGAATTCCACGTGTAGCGGTGAAATGCGTAGA GATGTGGAGGAACACCAGTGGCGAAGGCGACTCTCTGGTCTGTAACTGACGCTGAGGAGCGAAAGCGTGGGGAGCGAACA GGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTAGGGGGTTTCCGCCCCTTAGTGCTGCAGCT AACGCATTAAGCACTCCGCCTGGGGAGTACGGTCGCAAGACTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGT GGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCTCTGACAACCCTAGAGATAGGGCT TTCCCTTCGGGGACAGAGTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAAC GAGCGCAACCCTTGATCTTAGTTGCCAGCATTTAGTTGGGCACTCTAAGGTGACTGCCGGTGACAAACCGGAGGAAGGTG GGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTGCTACAATGGACAGAACAAAGGGCTGCGAGAC CGCAAGGTTTAGCCAATCCCATAAATCTGTTCTCAGTTCGGATCGCAGTCTGCAACTCGACTGCGTGAAGCTGGAATCGC TAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCACGAGAGTTTGT AACACCCGAAGTCGGTGAGGTAACCTTTATGGAGCCAGCCGCCGAA

Cell Structure, Metabolism and Life Cycle

Interesting features of cell structure; how it gains energy; what important molecules it produces.


Physiology and Pathogenesis

BIOCHEMICAL TEST RESULTS

  • Phenol Red Broth Tests: Glucose: positive; Lactose: negative; Sucrose: negative
  • Starch Hydrolysis Test: negative
  • Casein Hydrolysis Test: negative
  • Gelatin Hydrolysis Test: positive
  • DNA Hydrolysis Test: slight positive
  • Lipid Hydrolysis Test: positive
  • Methyl Red Test: negative
  • Voges Proskauer Test: negative
  • Citrate Test: positive
  • SIM Tests: negative for all
  • Nitrate Reduction: negative
  • Urea Hydrolysis: negative
  • Triple Sugar Iron Agar: negative for all
  • Oxidase Test: negative
  • Eosin Methylene Blue Agar (EMB) Test: positive
  • Hektoen Enteric Agar (HE) Test: negative
  • MacConkey Agar Test: negative
  • Decarboxylation Tests: Arginine: no change; Lysine: negative; Orinithine: negative
  • Phenylalanine Deaminase Test: negative
  • Catalase Test: negative
  • Blood Agar Test: slight positive
  • Mannitol Salt Agar (MSA) Test: negative
  • Phenylethyl Alcohol Agar (PEA) Test: negative
  • Bacitracin/Optochin Susceptibility Test: Bacitracin: resistant; Optochin: resistant
  • Bile Esculin Test: negative
  • 6.5% Salt Tolerance Test: slight positive


References

[Sample reference] Takai, K., Sugai, A., Itoh, T., and Horikoshi, K. "Palaeococcus ferrophilus gen. nov., sp. nov., a barophilic, hyperthermophilic archaeon from a deep-sea hydrothermal vent chimney". International Journal of Systematic and Evolutionary Microbiology. 2000. Volume 50. p. 489-500.

https://www.ncbi.nlm.nih.gov/pubmed/25410940, "Pseudomonas granadensis sp. nov., a new bacterial species isolated from the Tejeda, Almijara and Alhama Natural Park, Granada, Spain". PubMed.gov. Epub 2014 Nov 19.

Author

Page authored by Reggie Tuvilla and Maddy Gregory, students of Prof. Kristine Hollingsworth at Austin Community College.