Bacillus Pumilus RTMG
Classification
Domain: Bacteria
Phylum: Firmicutes
Class: Bacilli
Order: Bacillales
Family: Bacillaceae
Genus: Bacillus
Other Names:
Species
|
NCBI: Taxonomy |
Genus species: Bacillus pumilus
Habitat Information
Latitude: 30.398974 degrees Longitude: -97.704909 degrees
It was a clear day with a temperature of 65 F in the area of central Austin on January 25th 2018. A zip lock bag was used to collect the soil from the area mostly from the surface about a half inch deep. The location of the soil sample was taken from an area close to residential buildings underneath a tree. Rainfall was 0.0" and the pressure was 30.13 inHg. The description of the location was mostly Austin silty clay and urban land. 2-5% slopes and eroded.
Description and Significance
Describe the appearance (colonial and cellular), possible antimicrobial activity etc. of the organism, and why the organism might be significant.
Cellular: Gram positive
Colonial: rod-shape
Aerobic
Spore-Forming
Genome Structure
Describe the size and content of the genome. How many chromosomes? Circular or linear? Other interesting features? What is known about its sequence? Include S Ribosomal sequence that you obtained from PCR and sequencing here.
>RM1_4-16S-rRNA-SeqF_D09.ab1
ATTATTGGGCGTANGGGCTCGCAGGCGGTTTCTTAAGTCTGATGTGAAAGCCCCCGGCTCAACC GGGGAGGGTCATTGGAAACTGGGAAACTTGAGTGCAGAAGAGGAGAGTGGAATTCCACGTGTAGCGGTGAAATGCGTAGA GATGTGGAGGAACACCAGTGGCGAAGGCGACTCTCTGGTCTGTAACTGACGCTGAGGAGCGAAAGCGTGGGGAGCGAACA GGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTAGGGGGTTTCCGCCCCTTAGTGCTGCAGCT AACGCATTAAGCACTCCGCCTGGGGAGTACGGTCGCAAGACTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGT GGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCTCTGACAACCCTAGAGATAGGGCT TTCCCTTCGGGGACAGAGTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAAC GAGCGCAACCCTTGATCTTAGTTGCCAGCATTTAGTTGGGCACTCTAAGGTGACTGCCGGTGACAAACCGGAGGAAGGTG GGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTGCTACAATGGACAGAACAAAGGGCTGCGAGAC CGCAAGGTTTAGCCAATCCCATAAATCTGTTCTCAGTTCGGATCGCAGTCTGCAACTCGACTGCGTGAAGCTGGAATCGC TAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCACGAGAGTTTGT AACACCCGAAGTCGGTGAGGTAACCTTTATGGAGCCAGCCGCCGAA
Cell Structure, Metabolism and Life Cycle
Interesting features of cell structure; how it gains energy; what important molecules it produces.
Physiology and Pathogenesis
BIOCHEMICAL TEST RESULTS
- Phenol Red Broth Tests: Glucose: positive; Lactose: negative; Sucrose: posititve
- Starch Hydrolysis Test: positive
- Casein Hydrolysis Test: positive
- Gelatin Hydrolysis Test: positive
- DNA Hydrolysis Test: negative
- Lipid Hydrolysis Test: positive
- Methyl Red Test: negative
- Voges Proskauer Test: positive
- Citrate Test: positive
- SIM Tests: negative for all
- Nitrate Reduction: negative
- Urea Hydrolysis: positive
- Triple Sugar Iron Agar: negative for all
- Oxidase Test: positive
- Eosin Methylene Blue Agar (EMB) Test: negative
- Hektoen Enteric Agar (HE) Test: negative
- MacConkey Agar Test: negative
- Decarboxylation Tests: Arginine: negative; Lysine: negative; Orinithine: negative
- Phenylalanine Deaminase Test: negative
- Catalase Test: positive
- Blood Agar Test: Beta - Complete Breakdown
- Mannitol Salt Agar (MSA) Test: negative
- Phenylethyl Alcohol Agar (PEA) Test: Positive
- Bacitracin/Optochin Susceptibility Test: Bacitracin: resistant; Optochin: resistant
- Bile Esculin Test: positive
- 6.5% Salt Tolerance Test: negative
References
1. United States. National Center for Biotechnology Information. N.p., n.d. Bacillus pumilus: A ubiquitous soil organism. Web. 29 April 2016. http://www.ncbi.nlm.nih.gov/genome/?term=bacillus%20pumilus
2. Bacillus pumilus SAFR-032. N.p., n.d. Web. 27 April 2016. http://www.genome.jp/kegg-bin/show_organism?org=bpu
3. From, C., Hormazabal, V., and Granum, P. “Food poisoning associated with pumilacidin-producing Bacillus pumilus in rice”. International Journal of Food Microbiology. 2007. Volume 115. p. 319-324.
4. Tena, D., Martinez-Torres, J., Perez-Pomata, M., Saez-Nieto, J., Rubio, V., and Bisquert, J. “Cutaneous infection due to Bacillus pumilus: Report of 3 cases”. Clinical Infectious Diseases. 2007. Volume 44. P. e40-2.
Author
Page authored by Reggie Tuvilla and Maddy Gregory, students of Prof. Kristine Hollingsworth at Austin Community College.
