Bacillus coagulase

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Bacillus Coagulase

General:

B. coagulase is a lactic acid-forming bacterial species. After as a cause of an outbreak of coagulation in evaporated milk packed by an Iowa condensary. The organism was first isolated and described as Bacillus coagulans in 1915 by B.W. Hammer at the Iowa Agricultural Experiment Station

Environment: B. coagulase exists in humans. It can help in improving the vaginal flora, alleviating abdominal pain and reducing bloating in irritable bowel syndrome patients. Research suggests that Bacillus coagulans is effective in both treating as well as preventing recurrence of Clostridium difficile associated diarrhea.

Spores of the bacterium are activated in the acidic environment of the stomach and first start germinating and proliferating in the intestine. In some countries, Sporeforming B. coagulans strains are used as probiotics for patients on antibiotics.

Identification:

B. coagulans is a Gram-positive rod (0.9 by 3.0 to 5.0 μm in size). It may appear gram negative at times because the cell wall structure of endospore-forming bacteria is consistent with that of Gram-positive bacteria, and fresher cultures stain as expected. However, many sporeformers look Gram-negative when starting to grow.

It is catalase positive, spore-forming, motile, and a facultative anaerobe. It may look Gram-negative when entering the stationary phase of growth. The best temperature for growth is 50 °C (122 °F) ; and it can tolerate a temperature range of 30-55 °C (86-131 °F). It is IMViC tests VP and MR (methyl-red) positive.

Reference: https://en.wikipedia.org/wiki/Bacillus_coagulans http://textbookofbacteriology.net/Bacillus.html