Bacterial nucleation in pseudomonas syringae

Description

[Image 1]
Pseudomonas syringae shown using SEM. Source: Gordon Vrdoljak, Electron Microscopy Laboratory, U.C. Berkeley [1]

Importance

[Image 2]
Infection of European horse chestnut (Aesculus hippocastanum) with leasions caused by Pseudomonas syringae pv. aesculi. Source: Steele et al. 2010.

Ice Nucleation Active (INA) Proteins

[Image 3]
Pseudomonas syringae Cross section of modeled INP and a B-helical protein showing a wire frame representation of one loop. Cross section after 100 steps of energy minimization. Source: Graether and Jia, (2001).

[Figure 1]
Proportions of frozen droplets with respect to temperature at Pseudomonas syringae concentrations of 10⁷, 10⁶, 10⁵, 3.5⁴, and 3.5³. Cells were grown on nutrient agar and suspended in sterile water at appropriate concentrations. Source: Lindow et al., 1982.

[Figure 2]
Ice nucleation activity of corn leaf disks from plants with and without leaf surface populations of Pseudomonas syringae. Plants were sprayed with suspensions of 2 x 10⁸ cells/ml. Source: Lindow et al., 1982.

References

[1] http://genome.jgi-psf.org/psesy/psesy.home.html [2]

[2] Steele, H., B.E. Laue, G.A. MacAskill, A.J. Hendry, and S. Green. “Analysis of the natural infection of European horse chestnut (Aesculus hippocastanum) by Pseudomonas syringae pv. Aesculi.” Plant Pathology 59: 1005-1013.

[3] Graether, S.P., and Z. Jia. 2001. Modeling Pseudomonas syringae ice-nucleation protein as a B-helical protein. Biophysical Journal 80: 1169-1173.

[4] Lindow, S.E., D.C. Arny, and C.D. Upper. 1982. Bacterial ice nucleation: a factor in frost injury to plants. Plant Physiology 70: 1084-1089.

Edited by Ryan O'Connor,student of Joan Slonczewski for BIOL 238 Microbiology, 2011, Kenyon College.