Bacterial nucleation in pseudomonas syringae: Difference between revisions

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[[Image:Lindow et al.jpg|thumb|400px|right|[Figure 1] <br> Proportions of frozen droplets with respect to temperature at <i>Pseudomonas syringae</i> concentrations of 10<sup>7</sup>, 10<sup>6</sup>, 10<sup>5</sup>, 3.5<sup>4</sup>, and 3.5<sup>3</sup>. Cells were grown on nutrient agar and suspended in sterile water at appropriate concentrations. Source: Lindow <i>et al.</i>, 1982.]]
[[Image:Lindow et al.jpg|thumb|400px|right|[Figure 1] <br> Proportions of frozen droplets with respect to temperature at <i>Pseudomonas syringae</i> concentrations of 10<sup>7</sup>, 10<sup>6</sup>, 10<sup>5</sup>, 3.5<sup>4</sup>, and 3.5<sup>3</sup>. Cells were grown on nutrient agar and suspended in sterile water at appropriate concentrations. Source: Lindow <i>et al.</i>, 1982.]]


[[Image:Lindow_et_al_sterile.jpg|thumb|400px|right|[Figure 2] <br> Ice nucleation activity of corn leaf disks from plants with and without leaf surface populations of <br><i>Pseudomonas syringae</i>. Plants were sprayed with suspensions of 2 x 10<sup>8</sup> cells/ml. Source: Lindow <i>et al.</i>, 1982.]]
[[Image:Lindow_et_al_sterile.jpg|thumb|400px|right|[Figure 2] <br> Ice nucleation activity of corn leaf disks from plants with and without leaf surface populations of <i>Pseudomonas syringae</i>. Plants were sprayed with suspensions of 2 x 10<sup>8</sup> cells/ml. Source: Lindow <i>et al.</i>, 1982.]]


==Current Research==
==Current Research==

Revision as of 01:59, 24 April 2011

Introduction

Pseudomonas syringae

[Image 1]
Pseudomonas syringae shown using SEM. Source: Gordon Vrdoljak, Electron Microscopy Laboratory, U.C. Berkeley [1]
[Image 2]
Infection of European horse chestnut (Aesculus hippocastanum) with leasions caused by Pseudomonas syringae pv. aesculi. Source: Steele et al. 2010.

Ice Nucleation Active (INA) Proteins

[Image 3]
Pseudomonas syringae Cross section of modeled INP and a B-helical protein showing a wire frame representation of one loop. Cross section after 100 steps of energy minimization. Source: Graether and Jia, (2001).
[Figure 1]
Proportions of frozen droplets with respect to temperature at Pseudomonas syringae concentrations of 107, 106, 105, 3.54, and 3.53. Cells were grown on nutrient agar and suspended in sterile water at appropriate concentrations. Source: Lindow et al., 1982.
[Figure 2]
Ice nucleation activity of corn leaf disks from plants with and without leaf surface populations of Pseudomonas syringae. Plants were sprayed with suspensions of 2 x 108 cells/ml. Source: Lindow et al., 1982.

Current Research

Conclusion

References

[1] http://genome.jgi-psf.org/psesy/psesy.home.html [2]

[2] Steele, H., B.E. Laue, G.A. MacAskill, A.J. Hendry, and S. Green. “Analysis of the natural infection of European horse chestnut (Aesculus hippocastanum) by Pseudomonas syringae pv. Aesculi.” Plant Pathology 59: 1005-1013.

[3] Graether, S.P., and Z. Jia. 2001. Modeling Pseudomonas syringae ice-nucleation protein as a B-helical protein. Biophysical Journal 80: 1169-1173.

[4] Lindow, S.E., D.C. Arny, and C.D. Upper. 1982. Bacterial ice nucleation: a factor in frost injury to plants. Plant Physiology 70: 1084-1089.


Edited by Ryan O'Connor,student of Joan Slonczewski for BIOL 238 Microbiology, 2011, Kenyon College.