Bacterial nucleation in pseudomonas syringae: Difference between revisions

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==Species==
==Species==
As a Gram-negative, rod-shaped, obligate aerobic bacterium, Pseudomonas syringae is one of 78 species that has been described in the Pseudomonas genus (1). Characterized as an epiphyte, it grows supported non-parasitically by plants where it derives its nutrients and water from floating dust, rain, etc (9). This species can be found on tomatoes and beans to rice and tobacco, and is responsible for more surface frost damage to plants than any other mineral or organism (4). Its affiliation as a plant pathogen which causes disease in a large variety of plants makes this particular bacterium important in the field of food and biomass production, and is an important focus for the Department of Energy (2). P. syringae is a very stress-tolerant organism, and is the focus of many studies of stress-tolerant gene expression. Kurz et al., (2010) used biochemical approaches to address water stress tolerance in P. syringae (3). They showed that different osmolytes differentially contribute to water stress tolerance and interact at the level of transcription.
Different species of these flagellated, motile bacteria infect leaves and tissues of a wide range of hosts, but can be specific to a particular location of an individual. For example, Pseudomonas syringae pv. aesculi is able to infect vascular tissue to cause cankers in European horse chestnut in northwest Europe, but does not infect leaves, buds, or flowers on the same individual (Picture 2) (5). Some have also identified genes which are only expressed when the bacterium is on plants, which represent a ‘hidden genome’ not very well studied due to the absence in culture (2). Although there is variation with each pathovar, this bacterium grows optimally in cool, wet conditions from 15-25°C. It is proposed by Hirano et al. (1987) that momentum from falling raindrops is responsible for initiation of rapid growth and the forming of massive colonies on host surfaces (6). This has been seen as a method of how these bacterial cells are able to spread infection. This can also however, prevent infection by washing off bacterial cells from yet-infected surfaces.


[[Image:Pseudosmonas_syringae_SEM.jpg|thumb|400px|right|[Image 1]  
[[Image:Pseudosmonas_syringae_SEM.jpg|thumb|400px|right|[Image 1]  
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==Importance==
==Importance==
This particular species of Pseudomonas has been the subject of a large array of studies over the past 35 years with its involvement in crop freezing. The majority of frost-sensitive plants usually suffer from damage between -2 and -5°C (10, 11). When water gets this cold, water turns into ice in both inter- and intracellular ways, causing frost damage. P. syringae express a particular type of surface protein, ice-nucleation protein (INP), which increases temperatures to which water is able to freeze. In the absence of sites capable of ice nucleation, the cold water can supercool and freezing will not occur until the temperature is low enough for the most active ice nucleus available is able to catalyze crystallization of supercooled water (freezing)(12). Thus, supercooling instead of ice-nucleation could be a mechanism of frost protection.
P. syringae is a species of bacteria very important to present-day society, particularly with the ever-increasing population of planet earth. Chronic hunger affects 820 million people worldwide, killing 25,000 people every day, one child every eight seconds (13). Large quantities of crops are lost every year are lost due to frost such as in the state of Florida, where 40% of the world’s orange juice supply is grown (14). A large area of interest with Pseudomonas syringae focuses on genetically engineering these bacteria with ice-nucleation-minus proteins. Without these proteins it is thought the temperatures tolerable by plants may be decreased.


=Ice Nucleation Active (INA) Proteins=
=Ice Nucleation Active (INA) Proteins=

Revision as of 02:09, 24 April 2011

Introduction

Pseudomonas syringae

Species

As a Gram-negative, rod-shaped, obligate aerobic bacterium, Pseudomonas syringae is one of 78 species that has been described in the Pseudomonas genus (1). Characterized as an epiphyte, it grows supported non-parasitically by plants where it derives its nutrients and water from floating dust, rain, etc (9). This species can be found on tomatoes and beans to rice and tobacco, and is responsible for more surface frost damage to plants than any other mineral or organism (4). Its affiliation as a plant pathogen which causes disease in a large variety of plants makes this particular bacterium important in the field of food and biomass production, and is an important focus for the Department of Energy (2). P. syringae is a very stress-tolerant organism, and is the focus of many studies of stress-tolerant gene expression. Kurz et al., (2010) used biochemical approaches to address water stress tolerance in P. syringae (3). They showed that different osmolytes differentially contribute to water stress tolerance and interact at the level of transcription.

Different species of these flagellated, motile bacteria infect leaves and tissues of a wide range of hosts, but can be specific to a particular location of an individual. For example, Pseudomonas syringae pv. aesculi is able to infect vascular tissue to cause cankers in European horse chestnut in northwest Europe, but does not infect leaves, buds, or flowers on the same individual (Picture 2) (5). Some have also identified genes which are only expressed when the bacterium is on plants, which represent a ‘hidden genome’ not very well studied due to the absence in culture (2). Although there is variation with each pathovar, this bacterium grows optimally in cool, wet conditions from 15-25°C. It is proposed by Hirano et al. (1987) that momentum from falling raindrops is responsible for initiation of rapid growth and the forming of massive colonies on host surfaces (6). This has been seen as a method of how these bacterial cells are able to spread infection. This can also however, prevent infection by washing off bacterial cells from yet-infected surfaces.

[Image 1]
Pseudomonas syringae shown using SEM. Source: Gordon Vrdoljak, Electron Microscopy Laboratory, U.C. Berkeley [1]
[Image 2]
Infection of European horse chestnut (Aesculus hippocastanum) with leasions caused by Pseudomonas syringae pv. aesculi. Source: Steele et al. 2010.

Importance

This particular species of Pseudomonas has been the subject of a large array of studies over the past 35 years with its involvement in crop freezing. The majority of frost-sensitive plants usually suffer from damage between -2 and -5°C (10, 11). When water gets this cold, water turns into ice in both inter- and intracellular ways, causing frost damage. P. syringae express a particular type of surface protein, ice-nucleation protein (INP), which increases temperatures to which water is able to freeze. In the absence of sites capable of ice nucleation, the cold water can supercool and freezing will not occur until the temperature is low enough for the most active ice nucleus available is able to catalyze crystallization of supercooled water (freezing)(12). Thus, supercooling instead of ice-nucleation could be a mechanism of frost protection.

P. syringae is a species of bacteria very important to present-day society, particularly with the ever-increasing population of planet earth. Chronic hunger affects 820 million people worldwide, killing 25,000 people every day, one child every eight seconds (13). Large quantities of crops are lost every year are lost due to frost such as in the state of Florida, where 40% of the world’s orange juice supply is grown (14). A large area of interest with Pseudomonas syringae focuses on genetically engineering these bacteria with ice-nucleation-minus proteins. Without these proteins it is thought the temperatures tolerable by plants may be decreased.

Ice Nucleation Active (INA) Proteins

Description

[Figure 1]
Proportions of frozen droplets with respect to temperature at Pseudomonas syringae concentrations of 107, 106, 105, 3.54, and 3.53. Cells were grown on nutrient agar and suspended in sterile water at appropriate concentrations. Source: Lindow et al., 1982.

Structure

[Image 3]
Pseudomonas syringae Cross section of modeled INP and a B-helical protein showing a wire frame representation of one loop. Cross section after 100 steps of energy minimization. Source: Graether and Jia, (2001).
[Figure 2]
Ice nucleation activity of corn leaf disks from plants with and without leaf surface populations of Pseudomonas syringae. Plants were sprayed with suspensions of 2 x 108 cells/ml. Source: Lindow et al., 1982.

Bacterial Effects

Current Research

Conclusion

References

[1] http://genome.jgi-psf.org/psesy/psesy.home.html [2]

[2] Steele, H., B.E. Laue, G.A. MacAskill, A.J. Hendry, and S. Green. “Analysis of the natural infection of European horse chestnut (Aesculus hippocastanum) by Pseudomonas syringae pv. Aesculi.” Plant Pathology 59: 1005-1013.

[3] Graether, S.P., and Z. Jia. 2001. Modeling Pseudomonas syringae ice-nucleation protein as a B-helical protein. Biophysical Journal 80: 1169-1173.

[4] Lindow, S.E., D.C. Arny, and C.D. Upper. 1982. Bacterial ice nucleation: a factor in frost injury to plants. Plant Physiology 70: 1084-1089.


Edited by Ryan O'Connor,student of Joan Slonczewski for BIOL 238 Microbiology, 2011, Kenyon College.