Difference between revisions of "Baculoviridae"

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===Higher order taxa===
 
===Higher order taxa===
  
Virus; Baculoviridae; ''Granulovirus, Nucleopolyhedrovirus''
+
Virus; dsDNA viruses, no RNA stage; Baculoviridae
  
===Species===
+
===Genera===
  
''Autographa californica MNPV'', ''Trichoplusia ni SNPV'' (examples)
+
''Granulovirus, Nucleopolyhedrovirus''
  
 
==Description and Significance==
 
==Description and Significance==
  
Baculoviruses infect insect larvae, causing an infection which eventually turns the host organism into a liquid as the new viruses are released into the environment. The virus family has been known for hundreds of years, with the earliest accounts being about infections of Chinese silk worms. In the early 20th century, baculoviruses were suggested as a means of pest control, as it was discovered that a crystalline protein matrix protected virus particles when they were in an environment outside of a host organism. It wasn't until the 1980s, however, that there was a sucessful baculovirus-based pesticide, and more research is being done in this field of use. Currently, baculoviruses are used in biomedical research as well as in the study of virus-host interactions. There is also some discussion currently as to whether the genera in the Baculoviridae family should be reclassified. The reasons for this will be discussed in the next section.
+
Baculoviruses infect insect larvae, causing an infection which eventually turns the host organism into a liquid as the new viruses are released into the environment. The virus family has been known for hundreds of years, with the earliest accounts being about infections of Chinese silk worms. In the early 20th century, baculoviruses were suggested as a means of pest control, as it was discovered that a crystalline protein matrix protected virus particles when they were in an environment outside of a host organism. It wasn't until the 1980s, however, that there was a sucessful baculovirus-based pesticide, and more research is being done in this field of use. Currently, baculoviruses are used in biomedical research as well as in the study of virus-host interactions. There is also some discussion currently as to whether the genera in the Baculoviridae family should be reclassified. The reasons for this will be discussed in the next section. (source: [http://www-micro.msb.le.ac.uk/3035/kalmakoff/baculo/baculo.html Microbiology @ Leicester])
  
 
==Genome Structure==
 
==Genome Structure==
  
The baculovirus genome is non-segmented and contains a molecule of circular, double-stranded DNA. The complete genome sequence is 80000-180000 nucleotides long. Interspersed throughout the genome are sections of repetitive sequences of DNA known as ''homologous regions'', or ''hrs''. The complex structure of these ''hrs'' are formed by 60bp repeats, with each repeat containing a 28bp-long imperfect palindrome. These ''homologous regions'' enhance early transcription as well as act as origins for DNA replication. Many of the genes in the baculovirus genome overlap at the ends, which allows a large number of genes to be encoded in a small amount of DNA.
+
The baculovirus genome is non-segmented and contains a molecule of circular, double-stranded DNA. The complete genome sequence is 80000-180000 nucleotides long. Interspersed throughout the genome are sections of repetitive sequences of DNA known as ''homologous regions'', or ''hrs''. The complex structure of these ''hrs'' are formed by 60bp repeats, with each repeat containing a 28bp-long imperfect palindrome. These ''homologous regions'' enhance early transcription as well as act as origins for DNA replication. Many of the genes in the baculovirus genome overlap at the ends, which allows a large number of genes to be encoded in a small amount of DNA. (sources: [http://www.ncbi.nlm.nih.gov/ICTVdb/ICTVdB/06000000.htm ICTVdB] and [http://athena.bioc.uvic.ca/bioDoc/baculoviridae/ Viral Bioinformatics Resource Center])
  
Recent analysis of the genome sequence of baculoviruses suggests that the taxonomy of baculoviruses needs to be altered. More specifically, it has been discovered that the phylogeny of baculoviruses is more closely related to the classification of the host organism than the morphological traits of the virus, which had been used previously to classify baculoviruses.
+
Recent analysis of the genome sequence of baculoviruses suggests that the taxonomy of baculoviruses needs to be altered. More specifically, it has been discovered that the phylogeny of baculoviruses is more closely related to the classification of the host organism than the morphological traits of the virus, which had been used previously to classify baculoviruses. (source: [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=16648963&query_hl=2&itool=pubmed_docsum Jehle et al.] and [http://www.ncbi.nlm.nih.gov/ICTVdb/ICTVdB/06000000.htm ICTVdB])
  
 
==Viron Structure of a Baculovirus==
 
==Viron Structure of a Baculovirus==
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The two genera in the family Baculoviridae are definied by their different OV structure. ''Granulovirus'' OVs contain only one virion, and do not have a polyhedral envelope (known as a calyx). These OV are small, giving a "granular" appearance when many OVs are seen together. The protein that forms the crystalline matrix of ''Granulovirus'' OVs is known as ''granulin''. ''Nucleopolyhedrovirus'' OVs are much larger than ''Granulovirus'' OVs, holding 20 or more virions in each OV particle. The virions are either seperate or bundled together inside a calyx. The protein that forms the crystalline matrix of ''Nucleopolyhedrovirus'' OVs is called ''polyhedrin''.
 
The two genera in the family Baculoviridae are definied by their different OV structure. ''Granulovirus'' OVs contain only one virion, and do not have a polyhedral envelope (known as a calyx). These OV are small, giving a "granular" appearance when many OVs are seen together. The protein that forms the crystalline matrix of ''Granulovirus'' OVs is known as ''granulin''. ''Nucleopolyhedrovirus'' OVs are much larger than ''Granulovirus'' OVs, holding 20 or more virions in each OV particle. The virions are either seperate or bundled together inside a calyx. The protein that forms the crystalline matrix of ''Nucleopolyhedrovirus'' OVs is called ''polyhedrin''.
  
BVs are used for cell-to-cell transmission within an infected host. BV particles consist of a single capsid enclosed in an envelope which the capsid obtains when it "buds" out through the cell wall and into the host's system. Unlike OVs, BVs cannot survive outside the host organism.
+
BVs are used for cell-to-cell transmission within an infected host. BV particles consist of a single capsid enclosed in an envelope which the capsid obtains when it "buds" out through the cell wall and into the host's system. Unlike OVs, BVs cannot survive outside the host organism. (sources: [http://www.ncbi.nlm.nih.gov/ICTVdb/ICTVdB/06000000.htm ICTVdB] and [http://athena.bioc.uvic.ca/bioDoc/baculoviridae/ Viral Bioinformatics Resource Center])
  
 
==Reproductive Cycle of a Baculovirus in a Host Cell==
 
==Reproductive Cycle of a Baculovirus in a Host Cell==
Line 35: Line 35:
 
The OV, which is present in the environment, is transmitted through consumption. An insect larvae consumes an OV particle, which passes into the larvae's midgut. There the highly alkaline nature of the midgut dissolves the OV's protein matrix, releasing the virions. The virions attach to the epithelial cells of the midgut and infect them, using the host cell's DNA replication mechanisms to replicate itself. BV particles bud out through the cell membrane and spread throughout the host organism, causing a secondary infection. The BVs eventually infect the entire host organsim. Any cell infected by a BV creates large amounts of OVs, which are released through cell lysis. Due to the fact that so many cells are rupturing at the same time, the larvae is reduced to a milky-white liquid (a process known as ''liquefaction''). Eventually, the larvae ruptures, releasing the OVs into the environment.
 
The OV, which is present in the environment, is transmitted through consumption. An insect larvae consumes an OV particle, which passes into the larvae's midgut. There the highly alkaline nature of the midgut dissolves the OV's protein matrix, releasing the virions. The virions attach to the epithelial cells of the midgut and infect them, using the host cell's DNA replication mechanisms to replicate itself. BV particles bud out through the cell membrane and spread throughout the host organism, causing a secondary infection. The BVs eventually infect the entire host organsim. Any cell infected by a BV creates large amounts of OVs, which are released through cell lysis. Due to the fact that so many cells are rupturing at the same time, the larvae is reduced to a milky-white liquid (a process known as ''liquefaction''). Eventually, the larvae ruptures, releasing the OVs into the environment.
  
It should be noted that not all baculoviruses are lethal. Some baculoviruses set up persistent or even latent infections in healthy hosts. Transmission of such baculoviruses is vertical between hosts.
+
It should be noted that not all baculoviruses are lethal. Some baculoviruses set up persistent or even latent infections in healthy hosts. Transmission of such baculoviruses is vertical between hosts. (source: [http://athena.bioc.uvic.ca/bioDoc/baculoviridae/ Viral Bioinformatics Resource Center])
  
 
==Viral Ecology & Pathology==
 
==Viral Ecology & Pathology==
  
Baculoviruses infect insects. Most infections occur in a closely related species of insects, such as Lepidoptera. Baculoviruses achieve dispersal by passively interacting with other insects. Predators who consume infected insects, because of the fact that the acid environment of their stomachs will not dissolve the protein matrix surrounding OVs, disperse the virus through defecation. The soil is another reservoir for the virus. If the OVs can avoid solar UV radiation and alkaline conditions, then they can remain in the soil for a very long time.
+
Baculoviruses infect insects. Most infections occur in a closely related species of insects, such as Lepidoptera. Baculoviruses achieve dispersal by passively interacting with other insects. Predators who consume infected insects, because of the fact that the acid environment of their stomachs will not dissolve the protein matrix surrounding OVs, disperse the virus through defecation. The soil is another reservoir for the virus. If the OVs can avoid solar UV radiation and alkaline conditions, then they can remain in the soil for a very long time. (source: [http://www.trevorwilliams.info/Ecology_baculoviruses.htm Trevor Williams Homepage])
  
 
==References. Updated June 7, 2006==
 
==References. Updated June 7, 2006==
 
[http://www-micro.msb.le.ac.uk/3035/kalmakoff/baculo/baculo.html Microbiology @ Leicester: Virology: Baculoviruses]
 
  
 
[http://www.ncbi.nlm.nih.gov/ICTVdb/ICTVdB/06000000.htm ICTVdB - The Universal Virus Database, version 3. http://www.ncbi.nlm.nih.gov/ICTVdb/ICTVdB/]
 
[http://www.ncbi.nlm.nih.gov/ICTVdb/ICTVdB/06000000.htm ICTVdB - The Universal Virus Database, version 3. http://www.ncbi.nlm.nih.gov/ICTVdb/ICTVdB/]
  
[http://athena.bioc.uvic.ca/bioDoc/baculoviridae/ Viral Bioinformatics Resource Center & Viral Bioinformatics - Canada. Portal - Baculoviridae]
+
[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=16648963&query_hl=2&itool=pubmed_docsum Jehle, JA et al. "On the classification and nomenclature of baculoviruses: A proposal for revision."Arch Virol. 2006 May 2.]
  
[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=16648963&query_hl=2&itool=pubmed_docsum Jehle, JA et al. "On the classification and nomenclature of baculoviruses: A proposal for revision."Arch Virol. 2006 May 2.]
+
[http://www-micro.msb.le.ac.uk/3035/kalmakoff/baculo/baculo.html Microbiology @ Leicester: Virology: Baculoviruses]
  
 
[http://www.trevorwilliams.info/Ecology_baculoviruses.htm Trevor Williams Homepage: Biology and Ecology of Baculoviruses]
 
[http://www.trevorwilliams.info/Ecology_baculoviruses.htm Trevor Williams Homepage: Biology and Ecology of Baculoviruses]
 +
 +
[http://athena.bioc.uvic.ca/bioDoc/baculoviridae/ Viral Bioinformatics Resource Center & Viral Bioinformatics - Canada. Portal - Baculoviridae]

Revision as of 18:14, 9 June 2006

Electron micrograph of a baculovirus. From Marcus Drechsler, U. Bayreuth.

Baltimore Classification

Higher order taxa

Virus; dsDNA viruses, no RNA stage; Baculoviridae

Genera

Granulovirus, Nucleopolyhedrovirus

Description and Significance

Baculoviruses infect insect larvae, causing an infection which eventually turns the host organism into a liquid as the new viruses are released into the environment. The virus family has been known for hundreds of years, with the earliest accounts being about infections of Chinese silk worms. In the early 20th century, baculoviruses were suggested as a means of pest control, as it was discovered that a crystalline protein matrix protected virus particles when they were in an environment outside of a host organism. It wasn't until the 1980s, however, that there was a sucessful baculovirus-based pesticide, and more research is being done in this field of use. Currently, baculoviruses are used in biomedical research as well as in the study of virus-host interactions. There is also some discussion currently as to whether the genera in the Baculoviridae family should be reclassified. The reasons for this will be discussed in the next section. (source: Microbiology @ Leicester)

Genome Structure

The baculovirus genome is non-segmented and contains a molecule of circular, double-stranded DNA. The complete genome sequence is 80000-180000 nucleotides long. Interspersed throughout the genome are sections of repetitive sequences of DNA known as homologous regions, or hrs. The complex structure of these hrs are formed by 60bp repeats, with each repeat containing a 28bp-long imperfect palindrome. These homologous regions enhance early transcription as well as act as origins for DNA replication. Many of the genes in the baculovirus genome overlap at the ends, which allows a large number of genes to be encoded in a small amount of DNA. (sources: ICTVdB and Viral Bioinformatics Resource Center)

Recent analysis of the genome sequence of baculoviruses suggests that the taxonomy of baculoviruses needs to be altered. More specifically, it has been discovered that the phylogeny of baculoviruses is more closely related to the classification of the host organism than the morphological traits of the virus, which had been used previously to classify baculoviruses. (source: Jehle et al. and ICTVdB)

Viron Structure of a Baculovirus

Baculovirus virions have a complex structure which consists of an envelope and a rod-shaped nucleocapsid. The capsid is 200-450nm in length, and 30-100nm in diameter. The capsid has helical symmetry.

When the baculoviruses are extracellular, they can be found in two forms: budded virus (BV) and occluded virus (OV). OVs are polyhedral or oval-shaped crystalline protein matrices in which one or several mature virions are embedded. The OVs are large, measuring 0.15-15μm in length. OV particles are formed inside infected cells and are released when the cell lyses. The crystalline protein matrix of the OVprotects the virus while in the extracellular environment; because of this, OVs are used for transfer of the virus between hosts.

The two genera in the family Baculoviridae are definied by their different OV structure. Granulovirus OVs contain only one virion, and do not have a polyhedral envelope (known as a calyx). These OV are small, giving a "granular" appearance when many OVs are seen together. The protein that forms the crystalline matrix of Granulovirus OVs is known as granulin. Nucleopolyhedrovirus OVs are much larger than Granulovirus OVs, holding 20 or more virions in each OV particle. The virions are either seperate or bundled together inside a calyx. The protein that forms the crystalline matrix of Nucleopolyhedrovirus OVs is called polyhedrin.

BVs are used for cell-to-cell transmission within an infected host. BV particles consist of a single capsid enclosed in an envelope which the capsid obtains when it "buds" out through the cell wall and into the host's system. Unlike OVs, BVs cannot survive outside the host organism. (sources: ICTVdB and Viral Bioinformatics Resource Center)

Reproductive Cycle of a Baculovirus in a Host Cell

The OV, which is present in the environment, is transmitted through consumption. An insect larvae consumes an OV particle, which passes into the larvae's midgut. There the highly alkaline nature of the midgut dissolves the OV's protein matrix, releasing the virions. The virions attach to the epithelial cells of the midgut and infect them, using the host cell's DNA replication mechanisms to replicate itself. BV particles bud out through the cell membrane and spread throughout the host organism, causing a secondary infection. The BVs eventually infect the entire host organsim. Any cell infected by a BV creates large amounts of OVs, which are released through cell lysis. Due to the fact that so many cells are rupturing at the same time, the larvae is reduced to a milky-white liquid (a process known as liquefaction). Eventually, the larvae ruptures, releasing the OVs into the environment.

It should be noted that not all baculoviruses are lethal. Some baculoviruses set up persistent or even latent infections in healthy hosts. Transmission of such baculoviruses is vertical between hosts. (source: Viral Bioinformatics Resource Center)

Viral Ecology & Pathology

Baculoviruses infect insects. Most infections occur in a closely related species of insects, such as Lepidoptera. Baculoviruses achieve dispersal by passively interacting with other insects. Predators who consume infected insects, because of the fact that the acid environment of their stomachs will not dissolve the protein matrix surrounding OVs, disperse the virus through defecation. The soil is another reservoir for the virus. If the OVs can avoid solar UV radiation and alkaline conditions, then they can remain in the soil for a very long time. (source: Trevor Williams Homepage)

References. Updated June 7, 2006

ICTVdB - The Universal Virus Database, version 3. http://www.ncbi.nlm.nih.gov/ICTVdb/ICTVdB/

Jehle, JA et al. "On the classification and nomenclature of baculoviruses: A proposal for revision."Arch Virol. 2006 May 2.

Microbiology @ Leicester: Virology: Baculoviruses

Trevor Williams Homepage: Biology and Ecology of Baculoviruses

Viral Bioinformatics Resource Center & Viral Bioinformatics - Canada. Portal - Baculoviridae