Bradyrhizobium japonicum: Difference between revisions

From MicrobeWiki, the student-edited microbiology resource
No edit summary
 
(21 intermediate revisions by 4 users not shown)
Line 1: Line 1:
{{Curated}}
{{Biorealm Genus}}
{{Biorealm Genus}}


Line 10: Line 11:
Class: Alphaproteobacteria,
Class: Alphaproteobacteria,
Order: Rhizobiales,
Order: Rhizobiales,
Family: Bradyrhizobiaceae
Family: Bradyrhizobiaceae*1




Line 19: Line 20:




Edited by Erik Low, student of Rachel Larsen at UCSD.


===Genus===  
===Genus===  
Line 33: Line 33:
|}
|}
   
   
Edited by Erik Low, student of Rachel Larsen at UCSD.


==Description and significance==
==Description and significance==
Describe the appearance, habitat, etc. of the organism, and why it is important enough to have its genome sequenced.  Describe how and where it was isolated.
Include a picture or two (with sources) if you can find them.


''Bradyrhizobium japonicum'' is gram negative, rod shaped, nitrogen fixing bacteria that forms a symbiotic relationship with Glycine max, a soybean plant.  It is located on the root tips of the soy bean plant Glycine Max and eventually colonizes in the root nodules of the plant itself.  Within these root nodules, Bradyrhizobium japonicum is located in symbiosomes derived from the plant membrane.  One to several of these bacteria can inhabit a single symbiosome. In this symbiotic relationship, the plant provides a safe environment and a constant food supply such as carbon, which is used for growth and energy.  Such carbon sources come in the form of dicarboxylic acids, succinate, fumarate, and malate.  The bacteria in turn, provides the plant with fixed nitrogen, which is nitrogen gas that has been reduced and is readily usable by the plant.  This allows the plant to grow significantly in the absence of external fertilizer.  It is important to have the Bradyrhizobium japonicum's genome sequenced because manipulation of its genome can produce benefitial and desirable traits, which can improve soy bean crop production.  It was originally isolated from a soybean nodule in Florida, USA in 1957 through whole genome shotgun sequencing combined with bridging shotgun method.*4  
''Bradyrhizobium japonicum'' is gram-negative, rod shaped, nitrogen fixing bacteria that forms a symbiotic relationship with Glycine max, a soybean plant.  It is located on the root tips of the soy bean plant Glycine Max and eventually colonizes in the root nodules of the plant itself.  Within these root nodules, ''Bradyrhizobium japonicum'' is located in symbiosomes derived from the plant membrane.  One to several of these bacteria can inhabit a single symbiosome. In this symbiotic relationship, the plant provides a safe environment and a constant food supply such as carbon, which is used for growth and energy.  Such carbon sources come in the form of dicarboxylic acids, succinate, fumarate, and malate.  The bacteria in turn, provide the plant with fixed nitrogen, which is nitrogen gas that has been reduced and is readily usable by the plant.  This allows the plant to grow significantly in the absence of external fertilizer.  It is important to have t''he Bradyrhizobium japonicum's'' genome sequenced because manipulation of its genome can produce beneficial and desirable traits, which can improve soy bean crop production.  The bacterium was originally isolated from a soybean nodule in Florida, USA in 1957. The genome was sequenced by whole genome shotgun sequencing in 2002.*4
 
 
Edited by Erik Low, student of Rachel Larsen at UCSD.


==Genome structure==
==Genome structure==
Describe the size and content of the genome.  How many chromosomes?  Circular or linear?  Other interesting features?  What is known about its sequence?
Does it have any plasmids?  Are they important to the organism's lifestyle?


Bradyrhizobium japonicum has a complete genome. It is made up of a single circular chromosome with 9,105,828 base pairs in length and has no plasmids.  The average GC content was 64.1%.  It has 8317 potential protein coding genes, 1 set of rRNA genes, and 50 sets of tRNA genes.  There is a total of 167 genes coding for transposases with 104 insertion sequences in the genome.  DNA insertions of 4 kb to 97 kb in tRNA genes were found at 14 different locations in the genome.  This produced variant copies of the target tRNA genes.  These observations support the idea of B. japonicum genome's plasticity.  Its plasticity is probably due to homologous recombination and horizontal transfer and insertion of different DNA elements.*4
''Bradyrhizobium japonicum'' genome has been completely sequenced. It is made up of a single circular chromosome of 9,105,828 base pairs and has no plasmids.  The average GC content is 64.1%.  It has 8317 potential protein coding genes, 1 set of rRNA genes, and 50 sets of tRNA genes.  There is a total of 167 genes coding for transposases with 104 insertion sequences in the genome.  DNA insertions of 4 kb to 97 kb in tRNA genes were found at 14 different locations in the genome.  This produced variant copies of the target tRNA genes.  These observations support the idea of ''B. japonicum'' genome's plasticity.  Its plasticity is probably due to homologous recombination and horizontal transfer and insertion of different DNA elements.*4


Replicon Type: chromosome.
Replicon Type: chromosome.


Edited by Erik Low, student of Rachel Larsen at UCSD.


==Cell structure and metabolism==
==Cell structure and metabolism==
Describe any interesting features and/or cell structures; how it gains energy; what important molecules it produces.


Gram-negative soil bacteria of the family Rhizobiaceae such as Bradyrhizobium japonicum, synthesize a variety of cell-surface carbohydrates. These carbohydrates include lipopolysaccharides, capsular polysaccharides, exopolysaccharides (EPS), nodule polysaccharides, lipo chitin oligosaccharides, and cyclic -glucans, some of which may provide functions important to symbiosis.  It uses these carbohydrate structures to obtain the carbon energy sources from the soybean plant as well as gain entry.  
Gram-negative soil bacteria of the family Rhizobiaceae such as ''Bradyrhizobium japonicum'', synthesize a variety of cell-surface carbohydrates. These carbohydrates include lipopolysaccharides, capsular polysaccharides, exopolysaccharides (EPS), nodule polysaccharides, lipo chitin oligosaccharides, and cyclic -glucans, some of which may provide functions important to symbiosis.  It uses these carbohydrate structures to obtain the carbon energy sources from the soybean plant as well as gain entry. ''Bradyrhizobium japonicum'' uptakes the sugar trehalose the most rapidly and converts it to CO2.  Another energy source is UDP-Glucose which was taken up in large amounts but was very slowly metabolized.  Sucrose and glucose are also alternative energy sources, but they are metabolized at very low rates as well.*9 


Edited by Erik Low, student of Rachel Larsen at UCSD.


==Ecology==
==Ecology==
Describe any interactions with other organisms (included eukaryotes), contributions to the environment, effect on environment, etc.
 
''Bradyrhizobium japonicum'' has a symbiotic relationship with legumes, or more specifically soybean plants.  These bacteria are very beneficial to the environment as they promote the growth of the soybean plants.  It carries out a process called nitrogen fixation in the plant, so that the plant has a usable form of nitrogen.  This in turn causes the plant to grow rapidly since it has an abundance of readily usable nitrogen.  Promotion of plant growth causes more oxygen to be released into the environment, which is a crucial element for survival for most organisms. *4,5
 


==Pathology==
==Pathology==
''Bradyrhizobium japonicum'' uses its extracellular carbohydrate structures to gain entry into the host root cell.  It produces polysaccharide degrading enzymes in order to hydrolyze the cell wall.  Specifically polyalacturonase and variants of carboxymethylcellulase cleave glycosidic bonds of the host cell wall polymers.  These create erosion pits in the epidermal cells of the root, which allow for entry into the host root cells.  These enzymes are only secreted in highly localized areas where the bacteria is concentrated.  The reason for localization of these degradative enzymes is that it prevents excessive cell wall hydrolysis to the point where it either kills the host cells or causes a host cell immune reaction, which would ultimately hurt the bacteria.  In this way, it can back up away from the root cell if it senses that root cell degradation is proceeding to quickly.  However, it should be noted that the entry of Bradyrhizobium japonicum into root cells does not cause disease or damage because it has symbiotic relationship.  It has a symbiotic relationship where it fixes nitrogen for the plant, while the plant provides a safe environment and steady carbon source for it.*5   
 
''Bradyrhizobium japonicum'' uses its extracellular carbohydrate structures to gain entry into the host root cell.  It produces polysaccharide degrading enzymes in order to hydrolyze the cell wall.  Specifically polyalacturonase and variants of carboxymethylcellulase cleave glycosidic bonds of the host cell wall polymers.  These create erosion pits in the epidermal cells of the root, which allow for entry into the host root cells.  These enzymes are only secreted in highly localized areas where the bacteria are concentrated.  The reason for localization of these degradative enzymes is that it prevents excessive cell wall hydrolysis to the point where it either kills the host cells or causes a host cell immune reaction, which would ultimately hurt the bacteria.  In this way, it can back up away from the root cell if it senses that root cell degradation is proceeding too quickly.  However, it should be noted that the entry of ''Bradyrhizobium japonicum'' into root cells does not cause disease or damage because it has symbiotic relationship.  It has a symbiotic relationship where it fixes nitrogen for the plant, while the plant provides a safe environment and steady carbon source for it.*5   




Edited by Erik Low, student of Rachel Larsen at UCSD.


==Application to Biotechnology==
==Application to Biotechnology==
Does this organism produce any useful compounds or enzymes? What are they and how are they used?
This organism does not produce compounds or enzymes currently used in biotechnology, but it does carry out proccesses that are applicable to biotechnology.  It carries out the nitrogen fixation providing plants with a usable source of nitrogen.  This allows the soybean plants to grow in the absence of external fertilizers. Therefore if we can engineer or culture these microorganisms and incorporate it into plants, agriculture will flourish.  This will benefit the entire environment by providing bigger and better plants that will in turn give off oxygen.*4 
 


==Current Research==
==Current Research==
1.  Bradyrhizobium japonicum produces haloalkane dehalogenase, which degrades halogenated aliphatic pollutants.  The haloalkane dehalogenase that it produces has high catalytic activity for beta methylated haloalkanes and researchers are trying to discover the mechanism of its substrate specificity.*6  
1.  ''Bradyrhizobium japonicum'' produces haloalkane dehalogenase, which degrades halogenated aliphatic pollutants.  The haloalkane dehalogenase that it produces has high catalytic activity for beta methylated haloalkanes and researchers are trying to discover the mechanism of its substrate specificity.*6  
 
2.  Analysis of the diversity of rhizobia in different parts of China.  It was found that certain species of rhizobia were specific to certain geographic areas of China.  It was discovered that ''Bradyrhizobium japonicum'' was predominant in the nodules of Trifolium, which suggests that the bacteria is selected by both geographic features and legume hosts. *7


2.   
3Using rational genome mining, a mandelonitrile hydrolase was discovered in ''Bradyrhizobium japonicum''.  It was then transformed into ''E. coli'' where it was discovered to have a molecular mass of 37kDA.  It was also discovered that the mandelonitrile hydrolase was very effective in hydrolyzing mandelonitrile derivatives and converting mandelonitrile to mandelic acid.*8
Enter summaries of the most recent research here--at least three required


==References==
==References==
1.  [http://expasy.org/sprot/hamap/BRAJA.html, Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. ;
"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110.";
DNA Res. 9:189-197(2002)]
2.  [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi, Joe Bischoff, Mikhail Domrachev, Scott Federhen, Carol Hotton, Detlef Leipe, Vladimir Soussov, Richard Sternberg, Sean Turner.]
    [http://web.umr.edu/~djwesten/Bj.html, David J. Westenberg]
3.  [http://aem.asm.org/cgi/content/full/67/2/1011, Heather A. Louch1, and Karen J. Miller1,2,*
Intercollege Graduate Program in Genetics1 and Department of Food Science,2 The Pennsylvania State University, University Park, Pennsylvania 16802]
4.  [http://dnaresearch.oxfordjournals.org/cgi/reprint/9/6/189]
5.  [http://article.pubs.nrc-cnrc.gc.ca/ppv/RPViewDoc?issn=0008-4166&volume=47&issue=6&startPage=475]
6.  [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&dopt=AbstractPlus&list_uids=17401198&query_hl=3&itool=pubmed_docsum]
7. 


example:
1.  [http://expasy.org/sprot/hamap/BRAJA.html, Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. 2002. "Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110.". DNA Res. 9:189-197]
[http://www.pnas.org/cgi/content/full/100/14/8298 Glockner, F. O., M. Kube, M. Bauer, H. Teeling, T. Lombardot, W. Ludwig, D. Gade, A. Beck, K Borzym, K Heitmann, R. Rabus, H. Schlesner, R. Amann, and R. Reinhardt. 2003. "Complete genome sequence of the marine planctomycete ''Pirellula'' sp. strain 1."'' Proceedings of the National Acedemy of Sciences'', vol. 100, no. 14. (8298-8303)]
 
2.  [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi, Joe Bischoff, Mikhail Domrachev, Scott Federhen, Carol Hotton, Detlef Leipe, Vladimir Soussov, Richard Sternberg, Sean Turner. 2007. "Taxonomy Browser".]
 
3.  [http://aem.asm.org/cgi/content/full/67/2/1011, Heather A. Louch, and Karen J. Miller. 2000. "Synthesis of a Low-Molecular-Weight Form of Exopolysaccharide by Bradyrhizobium japonicum USDA 110". Applied and Environmental Microbiology. p. 1011-1014, Vol. 67, No. 2]
 
4.  [http://dnaresearch.oxfordjournals.org/cgi/reprint/9/6/189, Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. 2002. "Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110.". DNA Res. 9:189-197]
 
5.  [http://article.pubs.nrc-cnrc.gc.ca/ppv/RPViewDoc?issn=0008-4166&volume=47&issue=6&startPage=475, Pedro F. Mateos, David L. Baker, Maureen Petersen, Encarna Velázquez,José I. Jiménez-Zurdo, Eustoquio Martínez-Molina, Andrea Squartini,Guy Orgambide, David H. Hubbell, and Frank B. Dazzo. 2001. "Erosion of root epidermal cell walls by Rhizobium polysaccharide-degrading enzymes as related to primary host infection in the Rhizobium–legume symbiosis". Can. J. Microbiol. 47: 475–487]
 
6.  [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&dopt=AbstractPlus&list_uids=17401198&query_hl=3&itool=pubmed_docsum, Sato Y, Natsume R, Tsuda M, Damborsky J, Nagata Y, Senda T. 2007. "Crystallization and preliminary crystallographic analysis of a haloalkane dehalogenase, DbjA, from Bradyrhizobium japonicum USDA110.". PMID: 17401198.]
 
7.  [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&dopt=AbstractPlus&list_uids=17497134&query_hl=3&itool=pubmed_docsum, Liu XY, Wang ET, Li Y, Chen WX. 2007. "Diverse bacteria isolated from root nodules of Trifolium, Crotalaria and Mimosa grown in the subtropical regions of China.". PMID: 17497134]
 
8.  [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&dopt=AbstractPlus&list_uids=17350705&query_hl=3&itool=pubmed_docsum, Zhu D, Mukherjee C, Biehl ER, Hua L. 2007. "Discovery of a mandelonitrile hydrolase from Bradyrhizobium japonicum USDA110 by rational genome mining.". PMID: 17350705]
 
9.  [http://www.pubmedcentral.nih.gov/picrender.fcgi?artid=1056400&blobtype=pdf, SEPPO 0. SALMINEN, JOHN G. STREETER. 1986. "Uptake and Metabolism of Carbohydrates by Bradyrhizobium japonicum Bacteroids". Plant Physiol. 83, 535-540]




Edited by student of [mailto:ralarsen@ucsd.edu Rachel Larsen] and Kit Pogliano
Edited by Erik Low student of [mailto:ralarsen@ucsd.edu Rachel Larsen] and Kit Pogliano
KMG

Latest revision as of 15:47, 2 September 2015

This is a curated page. Report corrections to Microbewiki.

A Microbial Biorealm page on the genus Bradyrhizobium japonicum

Classification

Gram-negative nitrogen fixing bacteria

Higher order taxa

Domain: Bacteria, Phylum: Proteobacteria, Class: Alphaproteobacteria, Order: Rhizobiales, Family: Bradyrhizobiaceae*1


Strains: Bradyrhizobium japonicum strain USDA 110. Bradyrhizobium japonicum bv. genistearum. Bradyrhizobium japonicum bv. glycinearum.


Genus

Genus species: Bradyrhizobium japonicum


NCBI: Taxonomy


Description and significance

Bradyrhizobium japonicum is gram-negative, rod shaped, nitrogen fixing bacteria that forms a symbiotic relationship with Glycine max, a soybean plant. It is located on the root tips of the soy bean plant Glycine Max and eventually colonizes in the root nodules of the plant itself. Within these root nodules, Bradyrhizobium japonicum is located in symbiosomes derived from the plant membrane. One to several of these bacteria can inhabit a single symbiosome. In this symbiotic relationship, the plant provides a safe environment and a constant food supply such as carbon, which is used for growth and energy. Such carbon sources come in the form of dicarboxylic acids, succinate, fumarate, and malate. The bacteria in turn, provide the plant with fixed nitrogen, which is nitrogen gas that has been reduced and is readily usable by the plant. This allows the plant to grow significantly in the absence of external fertilizer. It is important to have the Bradyrhizobium japonicum's genome sequenced because manipulation of its genome can produce beneficial and desirable traits, which can improve soy bean crop production. The bacterium was originally isolated from a soybean nodule in Florida, USA in 1957. The genome was sequenced by whole genome shotgun sequencing in 2002.*4

Genome structure

Bradyrhizobium japonicum genome has been completely sequenced. It is made up of a single circular chromosome of 9,105,828 base pairs and has no plasmids. The average GC content is 64.1%. It has 8317 potential protein coding genes, 1 set of rRNA genes, and 50 sets of tRNA genes. There is a total of 167 genes coding for transposases with 104 insertion sequences in the genome. DNA insertions of 4 kb to 97 kb in tRNA genes were found at 14 different locations in the genome. This produced variant copies of the target tRNA genes. These observations support the idea of B. japonicum genome's plasticity. Its plasticity is probably due to homologous recombination and horizontal transfer and insertion of different DNA elements.*4

Replicon Type: chromosome.


Cell structure and metabolism

Gram-negative soil bacteria of the family Rhizobiaceae such as Bradyrhizobium japonicum, synthesize a variety of cell-surface carbohydrates. These carbohydrates include lipopolysaccharides, capsular polysaccharides, exopolysaccharides (EPS), nodule polysaccharides, lipo chitin oligosaccharides, and cyclic -glucans, some of which may provide functions important to symbiosis. It uses these carbohydrate structures to obtain the carbon energy sources from the soybean plant as well as gain entry. Bradyrhizobium japonicum uptakes the sugar trehalose the most rapidly and converts it to CO2. Another energy source is UDP-Glucose which was taken up in large amounts but was very slowly metabolized. Sucrose and glucose are also alternative energy sources, but they are metabolized at very low rates as well.*9


Ecology

Bradyrhizobium japonicum has a symbiotic relationship with legumes, or more specifically soybean plants. These bacteria are very beneficial to the environment as they promote the growth of the soybean plants. It carries out a process called nitrogen fixation in the plant, so that the plant has a usable form of nitrogen. This in turn causes the plant to grow rapidly since it has an abundance of readily usable nitrogen. Promotion of plant growth causes more oxygen to be released into the environment, which is a crucial element for survival for most organisms. *4,5


Pathology

Bradyrhizobium japonicum uses its extracellular carbohydrate structures to gain entry into the host root cell. It produces polysaccharide degrading enzymes in order to hydrolyze the cell wall. Specifically polyalacturonase and variants of carboxymethylcellulase cleave glycosidic bonds of the host cell wall polymers. These create erosion pits in the epidermal cells of the root, which allow for entry into the host root cells. These enzymes are only secreted in highly localized areas where the bacteria are concentrated. The reason for localization of these degradative enzymes is that it prevents excessive cell wall hydrolysis to the point where it either kills the host cells or causes a host cell immune reaction, which would ultimately hurt the bacteria. In this way, it can back up away from the root cell if it senses that root cell degradation is proceeding too quickly. However, it should be noted that the entry of Bradyrhizobium japonicum into root cells does not cause disease or damage because it has symbiotic relationship. It has a symbiotic relationship where it fixes nitrogen for the plant, while the plant provides a safe environment and steady carbon source for it.*5


Application to Biotechnology

This organism does not produce compounds or enzymes currently used in biotechnology, but it does carry out proccesses that are applicable to biotechnology. It carries out the nitrogen fixation providing plants with a usable source of nitrogen. This allows the soybean plants to grow in the absence of external fertilizers. Therefore if we can engineer or culture these microorganisms and incorporate it into plants, agriculture will flourish. This will benefit the entire environment by providing bigger and better plants that will in turn give off oxygen.*4


Current Research

1. Bradyrhizobium japonicum produces haloalkane dehalogenase, which degrades halogenated aliphatic pollutants. The haloalkane dehalogenase that it produces has high catalytic activity for beta methylated haloalkanes and researchers are trying to discover the mechanism of its substrate specificity.*6

2. Analysis of the diversity of rhizobia in different parts of China. It was found that certain species of rhizobia were specific to certain geographic areas of China. It was discovered that Bradyrhizobium japonicum was predominant in the nodules of Trifolium, which suggests that the bacteria is selected by both geographic features and legume hosts. *7

3. Using rational genome mining, a mandelonitrile hydrolase was discovered in Bradyrhizobium japonicum. It was then transformed into E. coli where it was discovered to have a molecular mass of 37kDA. It was also discovered that the mandelonitrile hydrolase was very effective in hydrolyzing mandelonitrile derivatives and converting mandelonitrile to mandelic acid.*8

References

1. Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. 2002. "Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110.". DNA Res. 9:189-197

2. Joe Bischoff, Mikhail Domrachev, Scott Federhen, Carol Hotton, Detlef Leipe, Vladimir Soussov, Richard Sternberg, Sean Turner. 2007. "Taxonomy Browser".

3. Heather A. Louch, and Karen J. Miller. 2000. "Synthesis of a Low-Molecular-Weight Form of Exopolysaccharide by Bradyrhizobium japonicum USDA 110". Applied and Environmental Microbiology. p. 1011-1014, Vol. 67, No. 2

4. Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. 2002. "Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110.". DNA Res. 9:189-197

5. Pedro F. Mateos, David L. Baker, Maureen Petersen, Encarna Velázquez,José I. Jiménez-Zurdo, Eustoquio Martínez-Molina, Andrea Squartini,Guy Orgambide, David H. Hubbell, and Frank B. Dazzo. 2001. "Erosion of root epidermal cell walls by Rhizobium polysaccharide-degrading enzymes as related to primary host infection in the Rhizobium–legume symbiosis". Can. J. Microbiol. 47: 475–487

6. Sato Y, Natsume R, Tsuda M, Damborsky J, Nagata Y, Senda T. 2007. "Crystallization and preliminary crystallographic analysis of a haloalkane dehalogenase, DbjA, from Bradyrhizobium japonicum USDA110.". PMID: 17401198.

7. Liu XY, Wang ET, Li Y, Chen WX. 2007. "Diverse bacteria isolated from root nodules of Trifolium, Crotalaria and Mimosa grown in the subtropical regions of China.". PMID: 17497134

8. Zhu D, Mukherjee C, Biehl ER, Hua L. 2007. "Discovery of a mandelonitrile hydrolase from Bradyrhizobium japonicum USDA110 by rational genome mining.". PMID: 17350705

9. SEPPO 0. SALMINEN, JOHN G. STREETER. 1986. "Uptake and Metabolism of Carbohydrates by Bradyrhizobium japonicum Bacteroids". Plant Physiol. 83, 535-540


Edited by Erik Low student of Rachel Larsen and Kit Pogliano KMG