Difference between revisions of "Canine Distemper Virus: Wildlife Conservation Implications"

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==Effect on Wildlife Conservation==
==Effect on Wildlife Conservation==
[[Image:Amur_leopard.jpg|thumb|400px|right|Figure 2. <ref name=Leopard> [https://phys.org/news/2018-01-canine-distemper-eastern-leopard-world.html]</ref>.]]
[[Image:Amur_leopard.jpg|thumb|400px|right|Figure 2. A critically endangered Amur leopard, populations of which have recently been infected by CDV, threatening the survival of this species.<ref name=Leopard> [https://phys.org/news/2018-01-canine-distemper-eastern-leopard-world.html]</ref>.]]

Revision as of 13:45, 11 April 2018

By Sam Lisak

Baltimore Classification

Group V: (−) sense single-stranded RNA viruse

Higher Order Taxa

Order: Mononegavirales

Family: Paramyxoviridae

Genus: Morbillivirus

Species: Canine Distemper Virus

Description and Significance

Canine distemper virus (CDV) is a viral pathogen that is commonly known for its ability to infect domestic dogs, though it affects many other species. This single-stranded, negative-sense RNA virus belongs to the Paramyxoviridae family and the genus Morbillivirus [1]. CDV is highly contagious, spreading through aerosol droplets and bodily fluids. Generally, the virus initially replicates in the respiratory tract where it enters the blood stream and spreads to lymphoid, nervous, and epithelial tissues [2]. While symptoms depend on the host species as well as the strain and severity of the virus, CDV often causes fever, ocular discharge, lethargy, anorexia, vomiting, diarrhea, seizure, paralysis, and the thickening of feet pads [3]. Although much research on this virus concentrates on its relationship to domestic dogs, CDV affects many mammal families such as Canidae (domestic and wild canids), Ursidae (bears), Pinnipeds (seals, sea lions, etc.), and Felidae (wild cats), to name a few [4]. Due to the wide range of potential hosts and the easy transmission pathway from domestic dogs to wildlife, CDV poses a significant threat to the conservation efforts of many threatened mammals. This report attempts to summarize current research on CDV as well as provide insight on how the virus affects wildlife, particularly, the conservation of threatened species.

Virion and Genome Structure

Figure 1. Viron and genome structure of Canine Distemper Virus[5].

All Morbilliviruses, including CDV, are virions enveloped by a lipid bilayer containing a single-stranded, negative-sense RNA genome [6]. CDV virus particles typically are between 150-300 nm in diameter [7].Their genomes code for six structural proteins in the following order: N (nucleocapsid), P (phosphoprotein). M (matrix protein). F (fusion protein). H (hemagglutinin protein), and L (large protein). They also code for V and C, two non-structural proteins [5]. The P and L proteins are coupled and attached to the N protein, which surrounds the genome to form the nucleocapsid. The P protein serves as the co-factor for the viral RNA-dependent RNA polymerase (L protein) [5]. The two glycoproteins, H and F, are spikes embedded in the lipid bilayer that mediate fusion between other cells. The envelope-associated M protein is evenly arranged underneath the lipid bilayer and regulates the two glycoproteins in addition to linking the nucleoplasmid with these proteins [5].

The CDV genome consists of 15,616 nucleotides and parallels the genomes of other morbilliviruses, particularly that of its closest relative, the measles virus (MV) [8]. This genome begins with the putative regulatory 3' leader (55 nucleotides long) that directly precedes the N gene, which is then followed by the P, M, F, H, and L genes. Between the L gene and the end of the CDV genome (the 5' terminus), lies the putative 5' leader region (38 nucleotides long) [9]. The putative sequences direct the transcription and replication of the genome. Intergenic sequences, located at the 3' and 5' ends of every structural gene segment control transcription termination and reinitiation [10].

Viral Ecology and Pathology

Include some current research in each topic, with at least one figure showing data.

Signs and Symptoms


Prevention and Treatment

Figure 2. Nobivac® DHP, a widely available CDV vaccine [12].

The prevention of CDV, while simple in theory, is almost impossible to successfully implement. Effective vaccines exist and are the only realistic solution for controlling canine distemper in domestic dogs [13]. While CDV vaccines have greatly decreased the prevalence on canine distemper, the disease still spreads among unvaccinated populations including animal shelters, pet stores, and feral dogs. Additionally, many domestic dogs are suceptible to CDV if their owner forgets or declines to vaccinate their puppies with the required booster shots that are needed until the puppy reaches 16 weeks [14]. Prevention of CDV in captive wild animals can be achieved through careful vaccination, however these vaccines must be proven to be safe for the animals as they can occasionally have adverse effects [13]. However, the widespread vaccination of wild animals is obviously unfeasible, so effective conservation and wildlife management strategies must be implemented to achieve prevention (possible solutions described below).

Despite the widespread prevalence of CDV vaccinations, there are currently no antiviral drugs or specific treatments for canine distemper [15]. Available treatments are symptomatic, aimed at controlling neurological dysfunction, maintaining fluid balance, and defend against secondary bacterial invasions [16]. However, there is hope for an antiviral medication after studies have shown the drugs ribavarin (RBV) and interferon-alpha (IFNα) inhibit the replication of CDV in vitro [15].

Effect on Wildlife Conservation

Figure 2. A critically endangered Amur leopard, populations of which have recently been infected by CDV, threatening the survival of this species.[17].

Include some current research in each topic, with at least one figure showing data.

Potential Conservation Solutions


Edited by student of Joan Slonczewski for BIOL 238 Microbiology, 2018, Kenyon College.