Citricoccus nitrophenolicus: Difference between revisions

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==Metabolism and Life Cycle==
==Metabolism and Life Cycle==
C. nitrophenolicus is a strict aerobe and grows best with pNP as its sole carbon, electron and energy source. Although the favored food source was pNP, growth was also observed when food sources such as short chain fatty acids, salicylate and a range of alcohols such as phenols and 4-cholorphenols were present. All these served as electron donors for growth.


- When pNP was used as the only source of carbon and energy:
Metabolism of pNP resulted in the release of nitrite


- When incubated with pNP and acetate:
pNP was metabolized first and then acetate


 
- When grown solely on acetate:
 
Both nitrite and nitrate served as oxygen sources where nitrate was quickly reduced to nitrite and therefore quickly accumulated in cultures during aerobic growth.
heterotrophs, anaerobically and microaerobically
 
require sulfur, thiosulfate or sulfate as electron acceptors
 
isolates grew well in yeast extract (carbon source)
    other sugars worked, but not as well (glycogen, gelatin, etc.)
large amounts of tetraether core lipids and trace amounts of diether core lipids.


==Ecology and Pathogenesis==
==Ecology and Pathogenesis==

Revision as of 17:15, 3 May 2013

Classification

Kingdom - Bacteria

Phylum - Actinobacteria

Class - Actinobacteridae

Order - Actinomycetales

Family - Micrococcineae

Genus - Micrococcaceae


Species

NCBI: Taxonomy

Citricoccus nitrophenolicus


Description and Significance

Citricoccus nitrophenolicus is a gram-posiitive, coccoid, non-motile aerobe that doesn't form endospores and metabolizes para-nitrophenol (pNP) into nitrite.

It was originally isolated from a wastewter treatment facility plant in Denmark, and by means of by means of selective enrichment using para-nitrophenol Citricoccus nitrophenolicus was isolated.

C. nitrophenolicus can grow at temperatures ranging from 15 to 32 degrees Celsius and at an optimum temperature of 27 degrees Celsius. Optimal growth was observed at a pH of 8 and ranged all the way from 6.8 to 10. C. nitrophenolicus was considered a halotolerant because of it's ability to grow at at salinity levels ranging from 3% to 17%.

When grown in the lab on agar plates, it exhibits bright yellow colonies, which are circular, convex and smooth.


Genome Structure

Describe the size and content of the genome. How many chromosomes? Circular or linear? Other interesting features? What is known about its sequence?

Metabolism and Life Cycle

C. nitrophenolicus is a strict aerobe and grows best with pNP as its sole carbon, electron and energy source. Although the favored food source was pNP, growth was also observed when food sources such as short chain fatty acids, salicylate and a range of alcohols such as phenols and 4-cholorphenols were present. All these served as electron donors for growth.

- When pNP was used as the only source of carbon and energy: Metabolism of pNP resulted in the release of nitrite

- When incubated with pNP and acetate: pNP was metabolized first and then acetate

- When grown solely on acetate: Both nitrite and nitrate served as oxygen sources where nitrate was quickly reduced to nitrite and therefore quickly accumulated in cultures during aerobic growth.

Ecology and Pathogenesis

Habitat; symbiosis; biogeochemical significance; contributions to environment.
If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.


References

[Sample reference] Takai, K., Sugai, A., Itoh, T., and Horikoshi, K. "Palaeococcus ferrophilus gen. nov., sp. nov., a barophilic, hyperthermophilic archaeon from a deep-sea hydrothermal vent chimney". International Journal of Systematic and Evolutionary Microbiology. 2000. Volume 50. p. 489-500.



http://ijsb.sgmjournals.org/content/49/3/1157.full.pdf+html [This is where I found all the above info, I will properly cite within the next day or two]


Author

Page authored by Cheryl Christie and Neha Rao, student of Prof. Jay Lennon at Michigan State University.

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