Clostridium Tagluense

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1. Classification

a. Higher order taxa

Higher Order Taxa: Bacteria; Firmicutes; Clostridia; Clostridiales; Clostridiaceae; Clostridium

Species: Clostridium tagluense

2. Description and significance

Clostridium tagluense is a Gram-positive, anaerobic, psychrotolerant bacteria that was first isolated from the permafrost of the Canadian Arctic Archipelago in 2009 [2]. C. tagluense belongs to the loosely defined genus of Clostridium, which is comprised of over one hundred species that are highly diverse both phenotypically and taxonomically [3]. C. tagluense is involved in ‘blown pack’ spoilage (BPS) of vacuum packaged beef [4,5]. C. tagluense can survive under extreme conditions, including temperatures from -40 to 10 degrees Celsius, and it can also form spores, making its growth difficult to control [2]. Due to these properties, C. tagluense contamination of meat processing plants can be potentially disruptive to food and meat packing industries, as it is highly resilient and is known to cause meat spoilage [4]. Also, due to its psychrotolerant nature and strict anaerobic metabolism, C. tagluense is proposed to have potential in industrial applications that involve synthesis of compounds at low-temperatures and production of biofuels [6].

3. Genome structure

C. tagluense has a high 16S rRNA sequence similarity (92-99%) to the species in Cluster I of Clostridium (Clostridium sensu stricto), which is defined as the representative species of the loosely defined Clostridium genus [2,7,8]. It’s genome hybridizes with less than 52% of other species within Cluster I of Clostridium, indicating that it is a separate species that has significant differences from other species of the genus 2. Cytosine and guanine comprise 31.5% of the genome, close to that of most species in Cluster I Clostridium [2].

4. Cell structure

Interesting features of cell structure. Can be combined with “metabolic processes”

5. Metabolic processes

Describe important sources of energy, electrons, and carbon (i.e. trophy) for the organism/organisms you are focusing on, as well as important molecules it/they synthesize(s). =6. Ecology and Pathology Clostridium tagluense is a psychrotolerant bacteria that grows in a temperature range from 0-28 degrees Celsius [2.]] . The microbe creates spherical endospores that allow it to withstand temperatures below freezing. C. tagluense has an optimal pH of 6.5-7.2 and an optimal NaCl concentration of 0-2% [2.]] . It is an obligate anaerobe that cannot grow in the presence of oxygen. These properties allow C. tagluense to reside in vacuum-sealed, frozen meat products and deep within permafrost [2.]] .

C. tagluense is not known to cause any human infections. Its main impact on human life lies in its ability to infect meat products. The microbe is known to cause blown-pack spoilage of vacuum-sealed and frozen meat [10.]] . This type of spoilage is unlikely to cause disease in humans because meat with this spoilage is typically disposed of before consumption [11.]] .

Strains of C. tagluense have been isolated from spoilage juices from beef and samples of lamb femur bones [4.]] [10.]] . The meat spoilage caused by C. tagluense can lead to legal and economic problems for the meat industry [11.]] . C. tagluense spores have been found to survive treatment by peroxyacetic acid and the vacuum-sealing and cooling processes of meat packing [12.]] . The best method to stop the growth of C. tagluense found is to lower the pH of its environment below levels it can withstand [4.]] .

Meat Spoilage Despite the fact that C. tagluense was first isolated from the permafrost of the Canadian Arctic Archipelago, increasing evidence indicates that the bacteria may be found in meat processing plants and involved in red-meat spoilage as well 2. In fact, a study surveying vacuum packed meat spoilage issues across the United Kingdom and in Ireland has found 4 out of 35 vacuum-packed meat samples with 16S rRNA sequence resembling that of C. tagluense 12. Moreover, studies disagree on the pH range that is required for the growth of C. tagluense. While some cite pH range of 6.0 - 7.2, which is generally outside the normal range of pH of vacuum packed meat of pH 5.5, growth under pH 5.5 has also been reported 5. This not only highlights that C. tagluense could proliferate in packaged meat if contaminated, but this also indicates that there are still unknowns regarding the growth characteristics of C. tagluense 2. The majority of current research on Clostridium tagluense primarily focuses on its prevalence and function in red-meat spoilage. Some research focuses on determining which substrates various psychrophilic or psychrotolerant clostridia, including C. tagluense, are able to utilize in order to grow. The microbe’s ability to cause spoilage of red meat is limited by the availability of fermentable substrates at the meat’s surface 4. More specifically, even though C. tagluense can normally metabolize glucose and glycogen, the microbe’s metabolism ceases when the pH drops below a certain level. It has been suggested that low pH is a likely cause of cessation of growth of C. tagluense, but more studies will need to be conducted in the future to determine the exact pH level that is necessary to inhibit C. tagluense growth 4. In addition, research also focuses on determining whether C. tagluense is able to utilize substrates such as amino acids or lactate in order to cause blown pack spoilage of red meat 4.

Cold-Adapted Applications In addition to research regarding the relevance of C. tagluense to meat spoilage, other research focuses on the potential applications of the antifreeze proteins found in the bacterial cell. These cold-adapted enzymes are being investigated for use in cold-water detergents, as well as for use cleaning polluted arctic soils 9. These applications of cold-adapted bacteria are relatively new, and thus present an exciting area of potential discovery. Further research needs to be conducted in this regard before C. tagluense can be exploited for industrial or for ecological purposes.

9. References

[1.] Taxonomy Browser - Clostridium Tagluense. Retrieved October 20, 2018, from

[2.] Suetin, S. V., Shcherbakova, V. A., Chuvilskaya, N. A., Rivkina, E. M., Suzina, N. E., Lysenko, A. M., & Gilichinsky, D. A. (2009). Clostridium tagluense sp. nov., a psychrotolerant, anaerobic, spore-forming bacterium from permafrost. International Journal of Systematic and Evolutionary Microbiology 59(6): 1421-1426.

[3.] Udaondo, Z., Duque, E., Ramos J. L. (2017). The pangenome of the genus Clostridium. Environmental Microbiology. 19(7): 2588-2603.

[4.] Yang, X., & Badoni, M. (2013). Substrate utilization during incubation in meat juice medium psychrotolerant clostridia associated with blown pack spoilage. Food Microbiology, 34(2): 400-405.

[5.] Yang, X., Youssef M. K., Gill C. O., Badoni M., Lopez-Campos O. (2014). Effects of meat pH on growth of 11 species of psychrotolerant clostridia on vacuum packaged beef and blown pack spoilage of the product. Food Microbiology. 39: 13-18.

[6.] Kumar, M., Gayen, K., (2011) Developments in biobutanol production: New Insights. Applied Energy. 88(6): 1999-2012.

[7.] Finegold, S. M., Song, Y., Liu, C. (2002) Taxonomy—General Comments and Update on Taxonomy of Clostridia and Anaerobic cocci. Anaerobe. 8(5): 283-285.

[8.] Collins, M. D., Lawson, P. A., Willems, A., Cordoba, J. J., Fernandez-Garayzabal, J., Garcia, P., Cai, J., Hippe, H., Farrow, J. A. E. & other authors (1994). The phylogeny of the genus Clostridium: proposal of five new genera and eleven new species combinations. Int J Syst Bacteriol. 44: 812–826.

[9.] Shcherbakova, V., Troshina, O. (2018). Biotechnological perspectives of microorganisms isolated from the Polar Regions. Microbiology Australia. Retrieved from

[10.] Brightwell, G., & Horváth, K. M. (2018). Molecular discrimination of New Zealand sourced meat spoilage associated psychrotolerant Clostridium species by ARDRA and its comparison with 16s RNA gene sequencing. Meat Science 138: 23-27.

[11.] Dorn-In, S., Schwaiger, K., Springer, C., Barta, L., Ulrich, S., Gareis, M. (2018). Development of a Multiplex qPCR for the Species Identification of Clostridium Estertheticum, C. Frigoriphilum, C. Bowmanii and C. Tagluense-like from Blown Pack Spoilage (BPS) Meats and from Wild Boars. International Journal of Food Microbiology 286: 162–169.

[12.] Cavill, L., Roneteriea-Monterrubio, A., Helps, C., Corry, J. (2011). Detection of cold-tolerant clostridia other than Clostridium estertheticum in raw vacuum-packed chill-stored meat. Food Microbiology 5(28): 957-963.

Edited by [Jennifer Talbot], student of Jennifer Talbot for BI 311 General Microbiology, 2016, Boston University.