Clostridium botulinum: Difference between revisions

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===Higher order taxa===
===Higher order taxa===


Bacteria; Firmicutes; Clostridia; Clostridiales; clostridiaceae
Domain: Bacteria  
 
Phylum: Firmicutes
 
Class: Clostridia
 
Order: Clostridiales
 
Family: Clostridiaceae
 
Genus: Clostridium


===Species===
===Species===
Line 17: Line 28:


==Description and significance==
==Description and significance==
The bacterium Clostridium botulinum is a rod-shaped organism of the genus Clostridium.  Most commonly found in soil, C. botulinum are found to grow most efficiently in low-oxygen conditions.  First discovered and isolated by Emile van Ermengem in 1896, C. botulinum survive by forming spores, remaining in a dormant state until environmental conditions arise that allow them to grow.  The importance of sequencing the genome of Clostridium Botulinum lies in its ability to produce a toxin known as botulin, one of the most powerful known toxins that lead to to the paralytic illness known as botulism.  
The bacterium ''Clostridium botulinum'' is a rod-shaped organism of the genus Clostridium.  Most commonly found in soil, ''Clostridium botulinum'' are found to grow most efficiently in low-oxygen conditions.  ''Clostridium botulinum'' was first discovered and isolated by Emile van Ermengem in 1896, and was later determined to survive by forming spores, remaining in a dormant state until environmental conditions arise that allow them to grow.  The importance of sequencing the genome of ''Clostridium Botulinum'' lies in its ability to produce a toxin known as botulin, one of the most powerful known toxins that leads to the paralytic illness known as botulism.  
 


Describe the appearance, habitat, etc. of the organism, and why it is important enough to have its genome sequenced. Describe how and where it was isolated.
 
Include a picture or two (with sources) if you can find them.
[http://www.affrc.go.jp/AVEM/Japanese/atlas/bacteria0.html]


==Genome structure==
==Genome structure==
The genome size of C. botulinum is estimated to be around 4,039 kbp, determined from the summation of restriction fragments of MluI, RsrII, and SmaI restriction endonuclease digestions.  The size of the chromosome of C. botulinum is relatively larger than many other gram-positive bacteria studied, possibly indicating that extra genomic requirements are needed for sporulation or the formation of disease-inducing toxins.  Genomic analysis by pulse-field gel electrophoresis revealed genes encoding neurotoxin, hemagglutinin A, and genese for a temperate phage, and various transposon Tn916 sites.
The genome size of ''C. botulinum'' is estimated to be around 4,039 kbp, determined from the summation of restriction fragments of MluI, RsrII, and SmaI restriction endonuclease digestions.  The size of the chromosome of ''C. botulinum'' is relatively larger than many other gram-positive bacteria studied, possibly indicating that extra genomic requirements are needed for sporulation or the formation of disease-inducing toxins.  Genomic analysis by pulse-field gel electrophoresis revealed genes encoding neurotoxin, hemagglutinin A, and genese for a temperate phage, and various transposon Tn916 sites.
 
Describe the size and content of the genome.  How many chromosomes?  Circular or linear?  Other interesting features?  What is known about its sequence?
Does it have any plasmids?  Are they important to the organism's lifestyle?


==Cell structure and metabolism==
==Cell structure and metabolism==
C. botulinum lies dormant in the form of spores until the right environmental conditions are met.  These spores are very resistant to adverse environmental effects, making them amenable to most environments and very hard to kill. The spores will grow under favorable conditions (anaerobiosis and substrate-rich environments) and will begin to produce their toxins as they rapidly propagate.  
''Clostridium botulinum'' is a gram-positive bacteria that is typically rod-shaped and arranged as singles, pairs, or chains.  ''C. botulinum'' lies dormant in the form of spores until the right environmental conditions are met.  These spores are very resistant to adverse environmental effects, making them amenable to most environments and very hard to kill. The spores will grow under favorable conditions (anaerobiosis and substrate-rich environments) and will begin to produce their toxins as they rapidly propagate.  
 
To prevent the occurrence of this bacterium in processed foods, many companies can their food with a pressurized boil to kill the bacterium with high temperatures.  Other techniques include high levels of oxygen, high acidity, high ratio of dissolved sugar, or very low levels of moisture. 
 


Describe any interesting features and/or cell structures; how it gains energy; what important molecules it produces.
To prevent the occurrence of this bacterium in processed foods, many companies can their food with a pressurized boil to kill the bacterium with high temperatures.  Other techniques include high levels of oxygen, high acidity, high ratio of dissolved sugar, or very low levels of moisture.


==Ecology==
==Ecology==
Describe any interactions with other organisms (included eukaryotes), contributions to the environment, effect on environment, etc.
The endospores that are produced from ''C. botulinum'' can survive in a vast array of environments until it meets the correct conditions to proliferate.  In order for proliferation to occur, the spores need to be present in non-halophilic salinity and anaerobic conditions.  ''C. botulinum'' can have multiple habitats, mainly in the soil and possibly food products.  This bacterium is also mesophilic, with an optimal temperature of around 37 degrees celsius.


==Pathology==
==Pathology==
Clostridium botulinum consists of seven different subtypes, labeled with the letters A-G.  While each of these subtypes produce a different botulin toxin, all of which, with the exception of C and D, are human pathogens.  Types A and B, which are commonly found in soil, are primarily the cause of botulism outbreaks in the United States, while Type E, which is found in fish, also contributes to cases of botulism in the country.
''Clostridium botulinum'' consists of seven different subtypes, labeled with the letters A-G.  Each of these subtypes produce a different botulin toxin, all of which, with the exception of C and D, are human pathogens.  Types A and B, which are commonly found in soil, are primarily the cause of botulism outbreaks in the United States, while Type E, which is found in fish, also contributes to cases of botulism in the country.


The botulinum toxin produced by this organism prevents the release of actylcholine at the neuromuscular junction, inducing paralysis by inhibiting muscle contraction.  A main cause of death from this disease involve death by asphyxiation, which is caused by the inability of the chest muscles to contract to facilitate breathing.   
The botulinum toxins most active forms are as dichain molecules, where heavy chains are linked to light chains via disulphide bonding.  Toxicity has appeared to be associated with the L chain, which blocks primarily on peripheral cholinergic synapses to prevent the calcium mediated release of acetylcholine, most likely interfering with the mechanism of exocytosis where vesicles containing neurotransmitters fuse with the cell membrane.  The toxin prevents the propagation of action potentials to the muscle fibers, inducing paralysis by inhibiting muscle contraction.  A main cause of death from this disease involve death by asphyxiation, which is caused by the inability of the chest muscles to contract to facilitate breathing.  Other effects of botulism, which affects both man and animals, include impaired vision and widespread muscular weakness.


How does this organism cause disease?  Human, animal, plant hosts? Virulence factors, as well as patient symptoms.
Currently there is no known cure for the toxic paralysis of botulism.  Although antitoxins do exist for this disease, by the time the symptoms of botulism show the toxin is irreversibly bound. At most the antitoxins may serve to neutralize the unbound toxin, but it is unable to reverse the binding of any toxins that are already exacting their effect.


==Application to Biotechnology==
==Application to Biotechnology==
Does this organism produce any useful compounds or enzymes? What are they and how are they used?
One conventional use of the toxins form ''C. botulinum'' include its effectiveness in treating a variety of conditions involving involuntary muscle spasm. A neurotoxin-haemagglutinin complex from Subtype A of ''Clostridium botulinum'' is used as a therapeutic toxin as an alternative to surgery in the treatment of strabismus and some focal dystonias. 


==Current Research==


Enter summaries of the most recent research here--at least three required


==References==
==References==
[Sample reference] [http://ijs.sgmjournals.org/cgi/reprint/50/2/489 Takai, K., Sugai, A., Itoh, T., and Horikoshi, K. "''Palaeococcus ferrophilus'' gen. nov., sp. nov., a barophilic, hyperthermophilic archaeon from a deep-sea hydrothermal vent chimney". ''International Journal of Systematic and Evolutionary Microbiology''. 2000. Volume 50. p. 489-500.]
 
Clostridium botulinum A A (Hall strain A (ATCC 3502)) (Project ID: 193) at Sanger Institute [In progress]
 
Mitchell WJ, Tewatia P, Meaden PG.
Genomic analysis of the phosphotransferase system in Clostridium botulinum.
J Mol Microbiol Biotechnol. 2007;12(1-2):33-42.
PMID: 17183209 [PubMed - in process]
 
Devriese, PP (1999) "On the discovery of Clostridium botulinum" J Hist Neurosci 8: 43-50
 
Lin, WJ, Johnson, EA
Genome analysis of Clostridium botulinum type A by pulsed-field gel electrophoresis
Appl. Environ. Microbiol. 1995 61: 4441-4447
 
Ryan KJ; Ray CG (editors) (2004). Sherris Medical Microbiology, 4th ed., McGraw Hill. ISBN 0838585299.  
 
Madigan M; Martinko J (editors). (2005). Brock Biology of Microorganisms, 11th ed., Prentice Hall. ISBN
 
 
Wells CL, Wilkins TD (1996). Botulism and Clostridium botulinum in: Baron's Medical Microbiology (Baron S et al, eds.), 4th ed., Univ of Texas Medical Branch. (via NCBI Bookshelf) ISBN 0-9631172-1-1
 
 


Edited by Brannon Peralta student of [mailto:ralarsen@ucsd.edu Rachel Larsen] and Kit Pogliano
Edited by Brannon Peralta student of [mailto:ralarsen@ucsd.edu Rachel Larsen] and Kit Pogliano

Latest revision as of 15:50, 15 April 2011

This is a curated page. Report corrections to Microbewiki.

A Microbial Biorealm page on the genus Clostridium botulinum

Classification

Higher order taxa

Domain: Bacteria

Phylum: Firmicutes

Class: Clostridia

Order: Clostridiales

Family: Clostridiaceae

Genus: Clostridium

Species

NCBI: Taxonomy

Clostridium Botulinum

Description and significance

The bacterium Clostridium botulinum is a rod-shaped organism of the genus Clostridium. Most commonly found in soil, Clostridium botulinum are found to grow most efficiently in low-oxygen conditions. Clostridium botulinum was first discovered and isolated by Emile van Ermengem in 1896, and was later determined to survive by forming spores, remaining in a dormant state until environmental conditions arise that allow them to grow. The importance of sequencing the genome of Clostridium Botulinum lies in its ability to produce a toxin known as botulin, one of the most powerful known toxins that leads to the paralytic illness known as botulism.


[1]

Genome structure

The genome size of C. botulinum is estimated to be around 4,039 kbp, determined from the summation of restriction fragments of MluI, RsrII, and SmaI restriction endonuclease digestions. The size of the chromosome of C. botulinum is relatively larger than many other gram-positive bacteria studied, possibly indicating that extra genomic requirements are needed for sporulation or the formation of disease-inducing toxins. Genomic analysis by pulse-field gel electrophoresis revealed genes encoding neurotoxin, hemagglutinin A, and genese for a temperate phage, and various transposon Tn916 sites.

Cell structure and metabolism

Clostridium botulinum is a gram-positive bacteria that is typically rod-shaped and arranged as singles, pairs, or chains. C. botulinum lies dormant in the form of spores until the right environmental conditions are met. These spores are very resistant to adverse environmental effects, making them amenable to most environments and very hard to kill. The spores will grow under favorable conditions (anaerobiosis and substrate-rich environments) and will begin to produce their toxins as they rapidly propagate.

To prevent the occurrence of this bacterium in processed foods, many companies can their food with a pressurized boil to kill the bacterium with high temperatures. Other techniques include high levels of oxygen, high acidity, high ratio of dissolved sugar, or very low levels of moisture.

Ecology

The endospores that are produced from C. botulinum can survive in a vast array of environments until it meets the correct conditions to proliferate. In order for proliferation to occur, the spores need to be present in non-halophilic salinity and anaerobic conditions. C. botulinum can have multiple habitats, mainly in the soil and possibly food products. This bacterium is also mesophilic, with an optimal temperature of around 37 degrees celsius.

Pathology

Clostridium botulinum consists of seven different subtypes, labeled with the letters A-G. Each of these subtypes produce a different botulin toxin, all of which, with the exception of C and D, are human pathogens. Types A and B, which are commonly found in soil, are primarily the cause of botulism outbreaks in the United States, while Type E, which is found in fish, also contributes to cases of botulism in the country.

The botulinum toxins most active forms are as dichain molecules, where heavy chains are linked to light chains via disulphide bonding. Toxicity has appeared to be associated with the L chain, which blocks primarily on peripheral cholinergic synapses to prevent the calcium mediated release of acetylcholine, most likely interfering with the mechanism of exocytosis where vesicles containing neurotransmitters fuse with the cell membrane. The toxin prevents the propagation of action potentials to the muscle fibers, inducing paralysis by inhibiting muscle contraction. A main cause of death from this disease involve death by asphyxiation, which is caused by the inability of the chest muscles to contract to facilitate breathing. Other effects of botulism, which affects both man and animals, include impaired vision and widespread muscular weakness.

Currently there is no known cure for the toxic paralysis of botulism. Although antitoxins do exist for this disease, by the time the symptoms of botulism show the toxin is irreversibly bound. At most the antitoxins may serve to neutralize the unbound toxin, but it is unable to reverse the binding of any toxins that are already exacting their effect.

Application to Biotechnology

One conventional use of the toxins form C. botulinum include its effectiveness in treating a variety of conditions involving involuntary muscle spasm. A neurotoxin-haemagglutinin complex from Subtype A of Clostridium botulinum is used as a therapeutic toxin as an alternative to surgery in the treatment of strabismus and some focal dystonias.


References

Clostridium botulinum A A (Hall strain A (ATCC 3502)) (Project ID: 193) at Sanger Institute [In progress]

Mitchell WJ, Tewatia P, Meaden PG. Genomic analysis of the phosphotransferase system in Clostridium botulinum. J Mol Microbiol Biotechnol. 2007;12(1-2):33-42. PMID: 17183209 [PubMed - in process]

Devriese, PP (1999) "On the discovery of Clostridium botulinum" J Hist Neurosci 8: 43-50

Lin, WJ, Johnson, EA Genome analysis of Clostridium botulinum type A by pulsed-field gel electrophoresis Appl. Environ. Microbiol. 1995 61: 4441-4447

Ryan KJ; Ray CG (editors) (2004). Sherris Medical Microbiology, 4th ed., McGraw Hill. ISBN 0838585299.

Madigan M; Martinko J (editors). (2005). Brock Biology of Microorganisms, 11th ed., Prentice Hall. ISBN


Wells CL, Wilkins TD (1996). Botulism and Clostridium botulinum in: Baron's Medical Microbiology (Baron S et al, eds.), 4th ed., Univ of Texas Medical Branch. (via NCBI Bookshelf) ISBN 0-9631172-1-1


Edited by Brannon Peralta student of Rachel Larsen and Kit Pogliano