DH5-Alpha E.coli: Difference between revisions
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Classification | Classification | ||
Domain | Domain: Bacteria | ||
Phylum: Protobacteria | |||
Class: Gammaproteobacteria | |||
Order: Enterobacteriales | |||
Family:Enterobacteriaceae | |||
Genus: Escherichia | |||
Species: Escherishia coli DH5-Alpha | |||
Description and significance | Description and significance | ||
This strain of E. Coli is not a pathogen, and was developed for laboratory cloning use. This strain was developed by D. Hanahan as a cloning strain with multiple mutations that enable high-efficiency transformations (Cite PDF). The mutations that the DH5-Alpha strain has are: dlacZ Delta M15 Delta(lacZYA-argF) U169 recA1 endA1 hsdR17(rK-mK+) supE44 thi-1 gyrA96 relA1 (cite PDF). These mutations correspond to the distinct characteristics that make the DH5-Alpha strain excel in laboratory cloning procedures. | |||
lacZ Delta M15 mutation: Allows for blue-white screening for recombinant cells. (http://bloodjournal.hematologylibrary.org/content/71/2/463.short) | |||
endA1 mutation: Allows for lower endonuclease degradation which ensures higher plasmid transfer rates. (http://www.ncbi.nlm.nih.gov/pubmed/18245285) | |||
recA1 mutation: reduces homologous recombination for a more stable insert. (http://www.ncbi.nlm.nih.gov/pubmed/6363880) | |||
Read more: http://wiki.answers.com/Q/What_is_the_significance_of_Ecoli_DH5_alpha#ixzz1buQk2cHN | |||
Describe the disease caused by this organism if it is a pathogen, or the natural macroscopic "field guide" appearance and habitat of your organism if it is not. What is or has been the impact your organism on human history or our environment?. How does it do this? How have we harnessed this power, or tried to prevent it? In other words, how do you know it if you see it, and how does its presence influence humans in the present, and historically? | Describe the disease caused by this organism if it is a pathogen, or the natural macroscopic "field guide" appearance and habitat of your organism if it is not. What is or has been the impact your organism on human history or our environment?. How does it do this? How have we harnessed this power, or tried to prevent it? In other words, how do you know it if you see it, and how does its presence influence humans in the present, and historically? | ||
Genome structure | Genome structure | ||
Revision as of 17:32, 26 October 2011
A Microbial Biorealm page on the genus BI246 General Microbiology Skidmore College Fall 2011 Contents
1 Classification 2 Description and significance 3 Genome structure 4 Cell structure, metabolism & life cycle 5 Ecology (including pathogenesis) 6 Interesting feature 7 References
Classification
Domain: Bacteria Phylum: Protobacteria Class: Gammaproteobacteria Order: Enterobacteriales Family:Enterobacteriaceae Genus: Escherichia Species: Escherishia coli DH5-Alpha
Description and significance
This strain of E. Coli is not a pathogen, and was developed for laboratory cloning use. This strain was developed by D. Hanahan as a cloning strain with multiple mutations that enable high-efficiency transformations (Cite PDF). The mutations that the DH5-Alpha strain has are: dlacZ Delta M15 Delta(lacZYA-argF) U169 recA1 endA1 hsdR17(rK-mK+) supE44 thi-1 gyrA96 relA1 (cite PDF). These mutations correspond to the distinct characteristics that make the DH5-Alpha strain excel in laboratory cloning procedures.
lacZ Delta M15 mutation: Allows for blue-white screening for recombinant cells. (http://bloodjournal.hematologylibrary.org/content/71/2/463.short) endA1 mutation: Allows for lower endonuclease degradation which ensures higher plasmid transfer rates. (http://www.ncbi.nlm.nih.gov/pubmed/18245285) recA1 mutation: reduces homologous recombination for a more stable insert. (http://www.ncbi.nlm.nih.gov/pubmed/6363880)
Read more: http://wiki.answers.com/Q/What_is_the_significance_of_Ecoli_DH5_alpha#ixzz1buQk2cHN
Describe the disease caused by this organism if it is a pathogen, or the natural macroscopic "field guide" appearance and habitat of your organism if it is not. What is or has been the impact your organism on human history or our environment?. How does it do this? How have we harnessed this power, or tried to prevent it? In other words, how do you know it if you see it, and how does its presence influence humans in the present, and historically?
Genome structure
Describe the size and content of the genome. How many chromosomes and plasmids? Circular or linear? Other interesting features? What is known about its sequence? Cell structure, metabolism & life cycle
Provide a physical and biochemical description of the organism. What kind of organism is it, what does it look like, how is it built, what are its metabolic properties, how can it be identified, what is it's life cycle, &c. In other words, describe the organism from its perspective. Ecology (including pathogenesis)
Describe its habitat, symbiosis, and contributions to environment. If it is a pathogen, how does this organism cause disease? Human, animal, plant hosts? Describe virulence factors and patient symptoms. Interesting feature
Describe in detail one particularly interesting aspect of your organism or it's affect on humans or the environment. References
[Sample reference] Takai, K., Sugai, A., Itoh, T., and Horikoshi, K. "Palaeococcus ferrophilus gen. nov., sp. nov., a barophilic, hyperthermophilic archaeon from a deep-sea hydrothermal vent chimney". International Journal of Systematic and Evolutionary Microbiology. 2000. Volume 50. p. 489-500.