DH5-Alpha E.coli: Difference between revisions

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The genomic structure of this strain is a singular circular chromosome consisting of 4,686,137 nucleotides, 4359 genes, and 4128 protein encoding genes (Cite http://www.ncbi.nlm.nih.gov/sites/entrez!db=genome&cmd=Retrieve&dopt=Overview&list_uids=22068)  
The genomic structure of this strain is a singular circular chromosome consisting of 4,686,137 nucleotides, 4359 genes, and 4128 protein encoding genes (Cite http://www.ncbi.nlm.nih.gov/sites/entrez!db=genome&cmd=Retrieve&dopt=Overview&list_uids=22068)  


This strain also contains numerous plasmids, and has the ability to accept plasmid insertion exceptionally well.   
This strain also contains plasmids, and has the ability to accept plasmid insertion exceptionally well.   





Revision as of 20:12, 26 October 2011

A Microbial Biorealm page on the genus BI246 General Microbiology Skidmore College Fall 2011 Contents

   1 Classification
   2 Description and significance
   3 Genome structure
   4 Cell structure, metabolism & life cycle
   5 Ecology (including pathogenesis)
   6 Interesting feature
   7 References

Classification

Domain: Bacteria Phylum: Protobacteria Class: Gammaproteobacteria Order: Enterobacteriales Family:Enterobacteriaceae Genus: Escherichia Species: Escherishia coli DH5-Alpha


Description and significance

This strain of E. Coli is not a pathogen, and was developed for laboratory cloning use. This strain was developed by D. Hanahan as a cloning strain with multiple mutations that enable high-efficiency transformations (Cite PDF). The mutations that the DH5-Alpha strain has are: dlacZ Delta M15 Delta(lacZYA-argF) U169 recA1 endA1 hsdR17(rK-mK+) supE44 thi-1 gyrA96 relA1 (cite PDF). These mutations correspond to the distinct characteristics that make the DH5-Alpha strain excel in laboratory cloning procedures.

lacZ Delta M15 mutation: Allows for blue-white screening for recombinant cells. (http://bloodjournal.hematologylibrary.org/content/71/2/463.short) endA1 mutation: Allows for lower endonuclease degradation which ensures higher plasmid transfer rates. (http://www.ncbi.nlm.nih.gov/pubmed/18245285) recA1 mutation: reduces homologous recombination for a more stable insert. (http://www.ncbi.nlm.nih.gov/pubmed/6363880)


Genome structure

The genomic structure of this strain is a singular circular chromosome consisting of 4,686,137 nucleotides, 4359 genes, and 4128 protein encoding genes (Cite http://www.ncbi.nlm.nih.gov/sites/entrez!db=genome&cmd=Retrieve&dopt=Overview&list_uids=22068)

This strain also contains plasmids, and has the ability to accept plasmid insertion exceptionally well.


Cell structure, metabolism & life cycle

Provide a physical and biochemical description of the organism. What kind of organism is it, what does it look like, how is it built, what are its metabolic properties, how can it be identified, what is it's life cycle, &c. In other words, describe the organism from its perspective. Ecology (including pathogenesis)

Describe its habitat, symbiosis, and contributions to environment. If it is a pathogen, how does this organism cause disease? Human, animal, plant hosts? Describe virulence factors and patient symptoms. Interesting feature

Describe in detail one particularly interesting aspect of your organism or it's affect on humans or the environment. References

[Sample reference] Takai, K., Sugai, A., Itoh, T., and Horikoshi, K. "Palaeococcus ferrophilus gen. nov., sp. nov., a barophilic, hyperthermophilic archaeon from a deep-sea hydrothermal vent chimney". International Journal of Systematic and Evolutionary Microbiology. 2000. Volume 50. p. 489-500.