Erwinia carotovora

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A Microbial Biorealm page on the genus Erwinia carotovora

Classification

Higher order taxa

Kingdom: Bacteria Phylum: Proteobacteria Class: Gammaproteobacteria Order: Enterobacteriales Family: Enterobacteriaceae Genus: Erwinia Species: carotovora

Species

Erwinia amylovora; Erwinia aphidicola; Erwinia billingiae; Erwinia carotovora; Erwinia chrysantum; Erwinia mallotivora; Erwinia papayae; Erwinia persicina; Erwinia psidii; Erwinia pyrifoliae; Erwinia rhapontici; Erwinia toletana; Erwinia tracheiphila

Description and significance

Erwinia carotovora is a rod shaped bacterium that was named after the crop of carrots from which it was first isolated. The bacterium infects a variety of vegetables and plants including carrots, potatoes, cucumbers, onions, tomatoes, lettuce and ornamental plants like iris.(2) These widespread microbes can be found in soil, guts of insects, water and suspended aerosols in air.(1) A major problem in agriculture, the microbes ceaselessly invade crops of potatoes and other vegetables in the fields or in storages that cause plant tissues to become soft and watery which eventually turn slimy and foul-smelling.(2) Whereas Erwinia carotovora subsp. atrosepticum’s pathogencity is restricted to potatoes in temperate temperatures, Erwinia carotovora subsp. carotovora infects a much broader host of plants, including potatoes, in warmer climates.(3)

Genome structure

Only the genome of Erwinia carotovora subsp. atroseptica strain SCRI1043 (Eca 1043) has been sequenced. The sequencing shows that the genome of Erwinia carotovora subsp. atroseptica is a single 5,064,019 base pair circular chromosome with 50.97 percent of guanine and cytosine, approximately 4,491 coding sequences (CDSs), seven rRNA operons, 76 tRNAs, and around 25 stable noncoding RNAs. It has acquired eleven horizontally acquired genomic islands (HAIs) that are predicted to allow nitrogen fixation, phenazine antibiotic production, phytotoxin synthesis and the production of adhesion proteins used for host attachment. In addition, the microbe acquired genes for opine catabolism. The bacterium contains all six Gram-negative protein secretion systems. Type one and type two secretion systems produce plant cell-wall degrading enzymes (PCWDEs) and other pathogenic determinants. There are twenty pectinase genes and seven CDCs that contribute to the production of PCWDEs. The type III secretion systems secrete proteins into eukaryotic cells where they contribute to pathogenesis by disrupting host cell signal transductions and cellular processes.(9) A mutation of the genes that resemble the type four secretion system locus of Agrobacterium tumefaciens negatively affects pathogenicity. The type five secretion system produces an auto transporter and the two-partner secretion systems may contribute to host cell attachment. (3)

Cell structure and metabolism

Erwinia Carotovora is a Gram-negative, rod-shaped bacterium that lives alone or aggregates into pairs and chains. Non-sporeforming and peritrichously flagellated, it is a facultative anaerobe that is catalase negative and oxidase positive. Erwinia carotovora produces a number of extracellular plant cell wall degrading enzymes such as pectic enzymes that degrade pectin, cellulase that degrades cellulose, hemicellulases, arabanases, cyanoses and a protease. As a mesophilic bacterium, Erwinia carotovora thrives the most in the temperature range between 27 and 30 degrees Celsius.(1) The genome sequencing of Erwinia carotovora subsp. atroseptica indicates that this microbe may not only be capable of nitrogen fixation and opine catabolism, but may also utilize around eighty ATP binding cassette transport systems.(3)

Ecology

In addition to infected plants, Erwinia carotovora can also be found in the guts of insects and bodies of water introduced by aerosols, runoff into rivers and dumping of potatoes. After rainfall upon diseased plants, an aerosol containing the bacteria is created. Fifty percent of the bacteria that become suspended in aerosols can survive for five to ten minutes and may travel for miles.(1)

Pathology

Erwinia carotovora is a plant pathogen that causes cell death through plant cell wall destruction by creating an osmotically fragile cell through the production of extracellular pectic enzymes and cellulase that break down integrity of the pectin and degrades cellulose. This organism causes soft-rot diseases of many plants, including potatoes through plant to plant infection, insect to plant infection, soil to plant and water to plant infection.

Application to Biotechnology

Does this organism produce any useful compounds or enzymes? What are they and how are they used?

Current Research

In the recent article "Genome sequence of the enterobacterial phytopathogen Erwinia carotovora subsp. atroseptica and characterization of virulence factors.", it was discovered that organism responsible for blackleg and soft-rot disease in potatoes, Erwinia Carotovora, demonstrates unexpected metabolic traits such as nitrogen fixation and opine catabolism. The proportion of genes responsible for the aforementioned features also encoded pathogenic determinants which were possibly aquired through horizontally transfered gene clusters of type IV secretion and polyketide phytotoxin synthesis.

References

Wood, M. 1998. Ubi7-new tool for potato breeders. Agricultural Research/January 1998, pp. 12-13.

Perombelon, Michael CM. The Prokaryotes. Second Edition. p2899- - 2921.

Bell KS, Sebaihia M, Pritchard L, Holden MT, Hyman LJ, Holeva MC, Thomson NR, Bentley SD, Churcher LJ, Mungall K, Atkin R, Bason N, Brooks K, Chillingworth T, Clark K, Doggett J, Fraser A, Hance Z, Hauser H, Jagels K, Moule S, Norbertczak H, Ormond D, Price C, Quail MA, Sanders M, Walker D, Whitehead S, Salmond GP, Birch PR, Parkhill J, Toth IK. "Genome sequence of the enterobacterial phytopathogen Erwinia carotovora subsp. atroseptica and characterization of virulence factors" Proceedings of the National Academy of Sciences of the United States of America 2004 Jul 27;101(30):11105-10. Epub 2004 Jul 19