https://microbewiki.kenyon.edu/index.php?title=File:Southernblottanalysis.png&feed=atom&action=historyFile:Southernblottanalysis.png - Revision history2024-03-29T06:08:17ZRevision history for this page on the wikiMediaWiki 1.39.6https://microbewiki.kenyon.edu/index.php?title=File:Southernblottanalysis.png&diff=87326&oldid=prevBellk: Only one mating type gene pair remains in the somatic nucleus. Southern blot analysis was carried out using whole-cell genomic DNA from a mature strain of each mating type (SB4208, SB4211, SB4214, SB4217, SB4220, and SB4223; see Table S1). The DNA was ...2013-05-08T21:58:27Z<p>Only one mating type gene pair remains in the somatic nucleus. Southern blot analysis was carried out using whole-cell genomic DNA from a mature strain of each mating type (SB4208, SB4211, SB4214, SB4217, SB4220, and SB4223; see Table S1). The DNA was ...</p>
<p><b>New page</b></p><div>Only one mating type gene pair remains in the somatic nucleus. Southern blot analysis was carried out using whole-cell genomic DNA from a mature strain of each mating type (SB4208, SB4211, SB4214, SB4217, SB4220, and SB4223; see Table S1). The DNA was digested with PvuII restriction endonuclease and separated by pulsed-field gel electrophoresis. Black segments, mating type-specific segment of each gene pair; diagonally hatched segments, conserved TM exons; arrows, PvuII sites; thin black bars, probes; size (kb) shown is that of the relevant PvuII fragment in the somatic genome (the corresponding germline PvuII fragments are not visible due to differences in size and copy number).doi:10.1371/journal.pbio.1001518.g004</div>Bellk