H. influenzae: Difference between revisions

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Describe the size and content of the genome.  How many chromosomes?  Circular or linear?  Other interesting features?  What is known about its sequence?
Describe the size and content of the genome.  How many chromosomes?  Circular or linear?  Other interesting features?  What is known about its sequence?


Haemophilus influenzae is a non-motile Gram-negative coccobacillus first identifed by Dr. Robert Pfeiffer in 1892. the genome structure of haemophilus influenzae consist of 1,830,138 nucleotide base pairs and it is estimated to have approximately 1740 genes and was the first genome to be sequenced and assembled in a free living organism (Fleischmann RD et al). It consist of a single circular chromosome replicon which has coding regions for rRNA, tRNA and proteins. They identified that the four nucleotides are not used at equal frequency across the genome as A and T are more common than C and G. there are sequence uncertainties in the genome  as other symbols occur in the sequence and corresponds to positions in the sequence that are not clearly one base or another. other bases which are seen in the sequence are Y, R, K, M, S, W and N. (Computational Genomics). because bacteria can transfer DNA from one specie to another by horizontal gene transfer, haemophilus influenzae takes up DNA by recognizing a 9- base pair sequence, 5'-AAGTGCGGT which is carried in multiple copies in its chromosome. there are 1465 copies of the 9-base pair DNA uptake sequence.the aligning of theses 9-base pair sequence sites have shown that there is an extended consensus region within the DNA of 29 base pairs containing the core 9-base pair region and also two 6-base pair A-T rich regions, each spaced one helix turn apart. most of the sites are inverted repeats(IR) located downstream to a gene terminus and are thus capable of forming loop structures in mRNA that signals for termination of transcription.
Haemophilus influenzae is a non-motile Gram-negative coccobacillus first identifed by Dr. Robert Pfeiffer in 1892. the genome structure of haemophilus influenzae consist of 1,830,138 nucleotide base pairs and it is estimated to have approximately 1740 genes and was the first genome to be sequenced and assembled in a free living organism (Fleischmann RD et al). It consist of a single circular chromosome replicon which has coding regions for rRNA, tRNA and proteins. They identified that the four nucleotides are not used at equal frequency across the genome as A and T are more common than C and G. there are sequence uncertainties in the genome  as other symbols occur in the sequence and corresponds to positions in the sequence that are not clearly one base or another. other bases which are seen in the sequence are Y, R, K, M, S, W and N. (Computational Genomics). because bacteria can transfer DNA from one specie to another by horizontal gene transfer, haemophilus influenzae takes up DNA by recognizing a 9- base pair sequence, 5'-AAGTGCGGT which is carried in multiple copies in its chromosome. there are 1465 copies of the 9-base pair DNA uptake sequence.the aligning of theses 9-base pair sequence sites have shown that there is an extended consensus region within the DNA of 29 base pairs containing the core 9-base pair region and also two 6-base pair A-T rich regions, each spaced one helix turn apart. most of the sites are inverted repeats(IR) located downstream to a gene terminus and are thus capable of forming loop structures in mRNA that signals for termination of transcription.(Smith OH, 1995 Jul 28;269(5223):538-40).


==Cell Structure, Metabolism and Life Cycle==
==Cell Structure, Metabolism and Life Cycle==

Revision as of 18:53, 17 April 2011

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Classification

Haemophilus influenzae is found along the bacteria lineage: kindgom name:Bacteria, phylum name:Proteobacteria,class name:Gamma Proteobacteria;order:Pasteurellales, family name:Pasteurellaceae; and genus name Haemophilus. the specie name is haemophilus influenzae

hamophilus influenzae has other names by which it can be identifed as it was formely known as the Pfeiffer's bacillus or Bacillus influenzae. it is also known as haemophilus meningitidis,Mycobacterium influenzae,Influenza-bacillus to name a few.

other species that fall under the same genus name are: haemophilus ducreyi and haemophilus influenzae aegyptius

Domain; Phylum; Class; Order; family [Others may be used. Use NCBI link to find]

Species

NCBI: Taxonomy

Genus species

Description and Significance

Describe the appearance, habitat, etc. of the organism, and why you think it is important.

Genome Structure

Describe the size and content of the genome. How many chromosomes? Circular or linear? Other interesting features? What is known about its sequence?

Haemophilus influenzae is a non-motile Gram-negative coccobacillus first identifed by Dr. Robert Pfeiffer in 1892. the genome structure of haemophilus influenzae consist of 1,830,138 nucleotide base pairs and it is estimated to have approximately 1740 genes and was the first genome to be sequenced and assembled in a free living organism (Fleischmann RD et al). It consist of a single circular chromosome replicon which has coding regions for rRNA, tRNA and proteins. They identified that the four nucleotides are not used at equal frequency across the genome as A and T are more common than C and G. there are sequence uncertainties in the genome as other symbols occur in the sequence and corresponds to positions in the sequence that are not clearly one base or another. other bases which are seen in the sequence are Y, R, K, M, S, W and N. (Computational Genomics). because bacteria can transfer DNA from one specie to another by horizontal gene transfer, haemophilus influenzae takes up DNA by recognizing a 9- base pair sequence, 5'-AAGTGCGGT which is carried in multiple copies in its chromosome. there are 1465 copies of the 9-base pair DNA uptake sequence.the aligning of theses 9-base pair sequence sites have shown that there is an extended consensus region within the DNA of 29 base pairs containing the core 9-base pair region and also two 6-base pair A-T rich regions, each spaced one helix turn apart. most of the sites are inverted repeats(IR) located downstream to a gene terminus and are thus capable of forming loop structures in mRNA that signals for termination of transcription.(Smith OH, 1995 Jul 28;269(5223):538-40).

Cell Structure, Metabolism and Life Cycle

Interesting features of cell structure; how it gains energy; what important molecules it produces.


Ecology and Pathogenesis

Habitat; symbiosis; biogeochemical significance; contributions to environment.
If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.

References

[Sample reference] Takai, K., Sugai, A., Itoh, T., and Horikoshi, K. "Palaeococcus ferrophilus gen. nov., sp. nov., a barophilic, hyperthermophilic archaeon from a deep-sea hydrothermal vent chimney". International Journal of Systematic and Evolutionary Microbiology. 2000. Volume 50. p. 489-500.





DNA uptake signal sequences in naturally transformable bacteria. Smith HO, Gwinn ML, Salzberg SL. Res Microbiol. 1999 Nov-Dec; 150(9-10):603-16.

Whole-genome random sequencing and assembly of Haemophilus influenzae Rd. Fleischmann RD, Adams MD, White O, Clayton RA, Kirkness EF, Kerlavage AR, Bult CJ, Tomb JF, Dougherty BA, Merrick JM. Science. 1995 Jul 28; 269(5223):496-512.

Frequency and distribution of DNA uptake signal sequences in the Haemophilus influenzae Rd genome. Smith HO, Tomb JF, Dougherty BA, Fleischmann RD, Venter JC.

Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.

Author

Page authored by _____, student of Prof. Doreen Cunningham at Saint Augustine's College.