Haloarcula hispanica: Difference between revisions

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{{Uncurated}}
=Classification=
=Classification=
Domain: Archaea  
Domain: Archaea;
Phylum: Euryarchaeota
Phylum: Euryarchaeota;
Class: Haloarchaea
Class: Haloarchaea;
Order: Halobacteriaies  
Order: Halobacteriaies;
Family: Halobacteriaceae
Family: Halobacteriaceae;
Genus: Haloarcula
Genus: ''Haloarcula''
==Species==
==Species==
Haloarcula hispanica
''Haloarcula hispanica'' (strain ATCC 33960)
 
=Description and significance=
=Description and significance=
Haloarcula Hispanica was isolated in 1986 in Spain from solar satern.
''Haloarcula hispanica'' was isolated in 1986 in Spain from a solar saltern, and was accepted as a new species in 1989 (Liu et al.,2011).
Haloarcula Hispanica is often grown in lab to be used to study SH1 virus that infects Haloarcula Hispanica.
''Haloarcula hispanica'' is often grown in lab to be used to study SH1 virus that infects ''Haloarcula hispanica''.  Additionally, many researchers have suggested that ''H. hispanica'' be used as a model archaeon for study. This is due to its low restriction activity compared to other members of its family, Halobacteriaceae. Apart from the study of host-virus interactions in saline environments, ''H. hispanica's'' metabolic pathways have been studied.  ''H. hispanica'' has been discovered to possess the ability to produce polyhydroxyalkanoates in nutrient-limiting conditions when carbon is in excess (Ding et al.,2014). PHAs are important for storing carbon and energy.  Furthermore, studies have been conducted to map out its sulfur metabolic pathway and discern amino acid biosynthesis.


[[File:HaloHisp1-multiz-phylo-tree.png‎|thumb|alt=Alt|Caption]]
=16S Ribosomal RNA Gene Information=
=16S Ribosomal RNA Gene Information=
  1 catgagcacg cgatgcgccg tagggaagcc gataagcgag ccgcctggga agtacgtccg
The most similar species to ''H. hispanica'' is the member of its genus, ''Holoarcula marismortui''. The 16S rRNA sequences of the two species have more than 99% similarity (Ding et al.,2014).
      61 caaggatgaa acttaaagga attggcgggg gagcaccaca accggaggag cctgcggttt
 
      121 aattggactc aacgccggac atctcaccgg tcccgacagt agtaatgaca gtcaggttga
=Genome Structure=
      181 cgactttact cgacgctact gagaggaggt gcatggccgc cgtcagctcg taccgtgagg
The strain ATCC 33960 has a genome of 3,890,005 bp consisting of two chromosomes (main chromosome 1 and minichromosome 2) and one megaplasmid (Liu et al., 2011).  The G+C content of the genome is greater than 62 percent, and nearly 4, 000 genes are protein-coding (Liu et al.,2011).  The genome also codes for 48 tRNAs, and 3 copies of 16S rRNA (Ding et al.,2014).  In a comparative analysis with another member of its genus, ''H. marismortui'', the two genomes featured 80 percent of proteins with counterparts in the other organism, while approximately 700 proteins were unique to ''H. hispanica'' (Arahal et al.,1996).
      241 cgtcctgtta agtcaggcaa cgagcgagac ccacacttct agttgccagc aacacccctg
The N601 strain is similar in length, G+C content, and number of protein-coding genes to ATCC 33960, but differs in that it possesses two plasmids (Ding et al.,2014).  Additionally, one clustered regularly interspaced short palindromic repeat (CRISPR) array is identified in N601.
      301 aggtggttgg gtacactagg aggactgcca ttgctaaagc ggaggaagga acgggcaacg
      361 gtaggtcagt atgccccgaa tggaccgggc aacacgcggg ctacaatggc tatgacagtg
      421 ggacgcaacg ccgagaggcg aagctaatct ccaaacgtag tcgtagttcg gattgcgggc
      481 tgaaacccgc ccgcatgaag ctggattcgg tagtaatcgc gtgtcagaag cgcgcggtga
      541 atacgtccct gctccttgca cacaccgccc gtcaaagcac ccgagtgggg tccggatgag
      601 gccgtcatgc gacggtcgaa tcgtca
=Genome Structure (if the genome exists)=
3,890,005 bp consisting of two chromosomes and one megaplasmid.  The G-C content of the genome is greater than 62 percent, and nearly 4, 000 genes are protein-coding.  In a comparative analysis with another member of its genus, H. marismortui, the two genomes featured 80 percent of proteins with counterparts in the other organism, while approximately 700 proteins were unique to H. hispanica.


=Cell structure and metabolism=
=Cell structure and metabolism=
The distinguishing characteristic of H. hispanica from other members of its genus are its unique polar lipids.  Cell growth occurs in triangle or square-shaped sheets of 65 cells.
The distinguishing characteristic of ''H. hispanica'' from other members of its genus are its unique polar lipids.  Cell growth occurs in triangle or square-shaped sheets of 65 cells (Juez et al.,1986).
 


=Ecology and Pathogenesis=
=Ecology and Pathogenesis=
The Genius Haloarcula is salt loving and often found in salt lakes,salty soil, and solar satern. the Genius Haloarcula requires at least 1.5 M NaCl for growth, but grow optimally in 2.0 to 4.5 M NaCl
The genus ''Haloarcula'' is salt loving and often found in salt lakes,salty soil, and solar saterns. The genus ''Haloarcula'' requires at least 1.5 M NaCl for growth, but grow optimally in 2.0 to 4.5 M NaCl.
The optimal temperature  for growth is 40-45 degrees celsius
The optimal temperature  for growth is 40-45 degrees Celsius (Liu et al,. 2011).
 
=Current Research=
=Current Research=
Current research is being done by Li et al. (2014). They found that to adapt the ''Haloarcula hispanica'' CRISPR-Cas system to a purified virus requires a priming process.


=References=
=References=
Soon to be updated
Arahal, D. R., Dewhirst, F. E., Paster, B. J., Volcani, B. E., & Ventosa, A. (1996). Phylogenetic analyses of some extremely halophilic archaea isolated from Dead Sea water, determined on the basis of their 16S rRNA sequences. Applied and Environmental Microbiology, 62(10), 3779-3786.
Ding, J. Y., Chiang, P. W., Hong, M. J., Dyall-Smith, M., & Tang, S. L. (2014). Complete genome sequence of the extremely halophilic archaeon ''Haloarcula hispanica'' strain N601. Genome announcements, 2(2), e00178-14.
 
Juez, G., Rodriguez-Valera, F., Ventosa, A., & Kushner, D. J. (1986). ''Haloarcula hispanica'' spec. nov. and ''Haloferax gibbonsii'' spec, nov., two new species of extremely halophilic archaebacteria. Systematic and Applied Microbiology, 8(1), 75-79.
 
Li, M., Wang, R., Zhao, D., & Xiang, H. (2014). Adaptation of the ''Haloarcula hispanica'' CRISPR-Cas system to a purified virus strictly requires a priming process. Nucleic Acids Research, 42(4), 2483-2492.
 
Liu, H., Wu, Z., Li, M., Zhang, F., Zheng, H., Han, J., & Xiang, H. (2011). Complete genome sequence of ''Haloarcula hispanica'', a model haloarchaeon for studying genetics, metabolism, and virus-host interaction. Journal of Bacteriology, 193(21), 6086-6087.


=Author=
=Author=
Page created by Max Pettit and Sebastian Howell students of Dr. Hidetoshi Urakawa, Florida Gulf Coast University

Latest revision as of 14:36, 4 October 2017

This student page has not been curated.

Classification

Domain: Archaea; Phylum: Euryarchaeota; Class: Haloarchaea; Order: Halobacteriaies; Family: Halobacteriaceae; Genus: Haloarcula

Species

Haloarcula hispanica (strain ATCC 33960)

Description and significance

Haloarcula hispanica was isolated in 1986 in Spain from a solar saltern, and was accepted as a new species in 1989 (Liu et al.,2011). Haloarcula hispanica is often grown in lab to be used to study SH1 virus that infects Haloarcula hispanica. Additionally, many researchers have suggested that H. hispanica be used as a model archaeon for study. This is due to its low restriction activity compared to other members of its family, Halobacteriaceae. Apart from the study of host-virus interactions in saline environments, H. hispanica's metabolic pathways have been studied. H. hispanica has been discovered to possess the ability to produce polyhydroxyalkanoates in nutrient-limiting conditions when carbon is in excess (Ding et al.,2014). PHAs are important for storing carbon and energy. Furthermore, studies have been conducted to map out its sulfur metabolic pathway and discern amino acid biosynthesis.


16S Ribosomal RNA Gene Information

The most similar species to H. hispanica is the member of its genus, Holoarcula marismortui. The 16S rRNA sequences of the two species have more than 99% similarity (Ding et al.,2014).

Genome Structure

The strain ATCC 33960 has a genome of 3,890,005 bp consisting of two chromosomes (main chromosome 1 and minichromosome 2) and one megaplasmid (Liu et al., 2011). The G+C content of the genome is greater than 62 percent, and nearly 4, 000 genes are protein-coding (Liu et al.,2011). The genome also codes for 48 tRNAs, and 3 copies of 16S rRNA (Ding et al.,2014). In a comparative analysis with another member of its genus, H. marismortui, the two genomes featured 80 percent of proteins with counterparts in the other organism, while approximately 700 proteins were unique to H. hispanica (Arahal et al.,1996). The N601 strain is similar in length, G+C content, and number of protein-coding genes to ATCC 33960, but differs in that it possesses two plasmids (Ding et al.,2014). Additionally, one clustered regularly interspaced short palindromic repeat (CRISPR) array is identified in N601.

Cell structure and metabolism

The distinguishing characteristic of H. hispanica from other members of its genus are its unique polar lipids. Cell growth occurs in triangle or square-shaped sheets of 65 cells (Juez et al.,1986).


Ecology and Pathogenesis

The genus Haloarcula is salt loving and often found in salt lakes,salty soil, and solar saterns. The genus Haloarcula requires at least 1.5 M NaCl for growth, but grow optimally in 2.0 to 4.5 M NaCl. The optimal temperature for growth is 40-45 degrees Celsius (Liu et al,. 2011).

Current Research

Current research is being done by Li et al. (2014). They found that to adapt the Haloarcula hispanica CRISPR-Cas system to a purified virus requires a priming process.

References

Arahal, D. R., Dewhirst, F. E., Paster, B. J., Volcani, B. E., & Ventosa, A. (1996). Phylogenetic analyses of some extremely halophilic archaea isolated from Dead Sea water, determined on the basis of their 16S rRNA sequences. Applied and Environmental Microbiology, 62(10), 3779-3786.

Ding, J. Y., Chiang, P. W., Hong, M. J., Dyall-Smith, M., & Tang, S. L. (2014). Complete genome sequence of the extremely halophilic archaeon Haloarcula hispanica strain N601. Genome announcements, 2(2), e00178-14.

Juez, G., Rodriguez-Valera, F., Ventosa, A., & Kushner, D. J. (1986). Haloarcula hispanica spec. nov. and Haloferax gibbonsii spec, nov., two new species of extremely halophilic archaebacteria. Systematic and Applied Microbiology, 8(1), 75-79.

Li, M., Wang, R., Zhao, D., & Xiang, H. (2014). Adaptation of the Haloarcula hispanica CRISPR-Cas system to a purified virus strictly requires a priming process. Nucleic Acids Research, 42(4), 2483-2492.

Liu, H., Wu, Z., Li, M., Zhang, F., Zheng, H., Han, J., & Xiang, H. (2011). Complete genome sequence of Haloarcula hispanica, a model haloarchaeon for studying genetics, metabolism, and virus-host interaction. Journal of Bacteriology, 193(21), 6086-6087.

Author

Page created by Max Pettit and Sebastian Howell students of Dr. Hidetoshi Urakawa, Florida Gulf Coast University