Jamie and Erika's Bacteria

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Classification

Because our PCR and gel electrophoresis were inconclusive, we were not able to perform a BLAST search for our DNA sequence and we are not 100% sure what bacteria it is.

Our DNA Hydrolysis test was positive (showed clearing) so we can assume that the bacteria contains DNA but we made a mistake during the electrophoresis loading procedures.

Species

NCBI: Taxonomy

Genus species: Unknown

Habitat Information

Describe the location and conditions under which the organism was isolated. File:Https://farm5.staticflickr.com/4562/38809350611 8118138bc5 c.jpg <img src ="https://farm5.staticflickr.com/4562/38809350611_8118138bc5_c.jpg">

Description and Significance

Cellular Morphology: The bacteria is a Gram-negative bacillus that showed mostly singular bacilli and a few diplobacillus throughout. It is non-motile. Our capsule stain was negative and the Endospore stain was positive.

Colony Morphology: The bacteria presented entire margins, creamy white, slightly yellow/tan, opaque, shiny in appearance with no extracellular staining. Isolated colonies were round, moist looking and slightly raised. This bacterium did present a slight odor.

The bacteria shoed no sensitivity to Bacitracin or Optichin disks, it grew as normal in the agar. It was also tested for a sensitivity to Axlocillin, Ceftazidines, Ampicillin, Oxacillin, and Vancomycin. Out of these five, only Ceftazidine presented a zone of inhibition to the growth of the bacteria. Vancomycin showed a very slight zone of inhibition, but probably not enough to be significant in fighting the bacteria.

, and why the organism might be significant.

Genome Structure

Since our PCR and gel electrophoresis techniqes were inconclusive, we cant be sure what our genome structure consists of. Since it is a bacteria we can assume it would have a circular chromosome.

Cell Structure, Metabolism and Life Cycle

Since our bacteria was positive for the Oxidase test we can assume it uses the electron transport chain for energy. It also appears to be aerobic.

Physiology and Pathogenesis

Selective Media Test Results:

Hektoen Enteric Agar = Negative / No Growth

MacConkey Agar = Negative / No Growth

Mannitol Salt Agar = Negative / No Growth

6.5% Salt Broth = Negative / No Growth

Phenylethyl Alcohol Agar = Negative / No Growth

Eosin Methylene Blue Agar = Positive for growth, mucoid pink growth indicates weak lactose fermentation

Differential Tests:

Blood Agar = Gamma Hemolysis (no clearing)

Nitrate Reduction = Negative for Nitrate reduction abilities

Triple Sugar Iron Agar = No fermentation / No gas production

Citrate Test = Negative

MR VP = Negative / Negative

Phenol Red Broth = No fermentation / No gas

Decarboxylation Test = Negative for all three substrates

Phenylalanine Deaminase = Negative

SIM Test = Negative for motility / Negative for tryptophanase / Negative sulfur reduction

Catalase Test = Postive

Oxidase Test = Positive

Bile Esculine = Positive

Gelatin Hydrolysis = Negative

Lipid Hydrolysis = Positive

Starch Hydrolysis = Negative

DNA Hydrolysis = Positive

Urea Hydrolysis = Negative

No known enzymes as of now.No known contributions to the environment as of now.

Diseases and symptoms unknown as of right now.

References

[Sample reference] Takai, K., Sugai, A., Itoh, T., and Horikoshi, K. "Palaeococcus ferrophilus gen. nov., sp. nov., a barophilic, hyperthermophilic archaeon from a deep-sea hydrothermal vent chimney". International Journal of Systematic and Evolutionary Microbiology. 2000. Volume 50. p. 489-500.

Author

Page authored by Jamie Powell and Erika Anderson, students of Prof. Kristine Hollingsworth at Austin Community College.