Lutibacter aestuarii sp. nov., isolated from a tidal flat sediment, and emended description of the genus Lutibacter Choi and cho 2006.
Classification; New taxa Bacteroidetes, Bacteria;Lutibacter aestuarii, phylum; Bacteroidetes, family; Flavobacteriaceae, genus; Lutibacter Species; Aestuarii
Description and significance: "A gram-negative, non-spore-forming, non-motile rod, designated MA-My1(T), was isolated from a tidal flat sediment of the South Sea, Korea. Strain MA-My1(T) grew optimally at pH 7.0-7.5, at 30 °C and with 2.0-3.0 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain MA-My1(T) clustered with Lutibacter litoralis CL-TF09(T) and Lutibacter maritimus S7-2(T), with which it exhibited 97.3 and 95.3 % 16S rRNA gene sequence similarity, respectively. Strain MA-My1(T) contained MK-6 as the predominant menaquinone and iso-C(15 : 0), iso-C(15 : 0) 3-OH and iso-C(16 : 0) 3-OH as the major fatty acids. The major polar lipids were phosphatidylethanolamine and two unidentified lipids. The DNA G+C content was 30.6 mol% and DNA-DNA relatedness between strain MA-My1(T) and L. litoralis JCM 13034(T) was 6.3±0.8 %. The differential phenotypic properties, together with phylogenetic and genotypic distinctiveness, distinguished strain MA-My1(T) from the members of the genus Lutibacter. On the basis of the data presented, strain MA-My1(T) is considered to represent a novel species of the genus Lutibacter, for which the name Lutibacter aestuarii sp. nov. is proposed. The type strain is MA-My1(T) ( = KCTC 23499(T) = CCUG 60022(T). The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain MA-My1T is HM234096". "The genus Lutibacter (family Flavobacteriaceae, phylum Bacteroidetes) was first proposed by Choi & Cho (2006)and, at the time of writing, comprises two species with validly published names, Lutibacter litoralis (type species;Choi & Cho, 2006) and Lutibacter maritimus (Park et al., 2010). The genus is distinguished from the phylogenetically related genera Tenacibaculum and Polaribacter by 16S rRNA gene sequence phylogeny, phenotypic properties and fatty acid compositions (Choi & Cho, 2006). The two recognized Lutibacter species were isolated from tidal flat sediments on the west coast of the Korean peninsula (Choi & Cho, 2006; Park et al., 2010). Recently, a Lutibacter-like bacterial strain, designated MA-My1T, was also isolated from a tidal flat, but on the south coast of the peninsula. Strain MA-My1T was isolated by the dilution-plating technique at 25 uC on marine agar 2216 (MA; Difco). The isolate was maintained on MA at 4 uC and stored in 20 % (w/v) glycerol in distilled water at –80 uC. L. litoralis JCM13034T was obtained from the JCM (Saitama, Japan) and used as a reference strain for DNA–DNA hybridization. The morphological, physiological and biochemical characteristics of strain MA-My1T were investigated after cultivation on MA at 30 uC. Cell morphology was examined by light microscopy (E600; Nikon) and transmission electron microscopy (CM-20; Philips). The presence of flagella was assessed by transmission electron microscopy on cells from an exponentially growing culture. For this purpose, cells were negatively stained with 1 % (w/v) phosphotungstic acid and grids were examined after being air-dried. Gliding motility was investigated as described by Bowman (2000). The Gram reaction was determined using the bioMerieux".
References: http://www.ncbi.nlm.nih.gov/pubmed/21460139, http://ijs.sgmjournals.org/content/62/2/420.full.pdf+html, Lee,S.Y., Lee,M.H., Oh,T.K. and Yoon,J.H. Edited by (Ahmed Alneemi), student of Rachel Larsen at the University of Southern Maine [Category:Pages edited by students of Rachel Larsen]]