Marinobacter aquaeolei: Difference between revisions

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Does it have any plasmids?  Are they important to the organism's lifestyle?
Does it have any plasmids?  Are they important to the organism's lifestyle?


''Marino aquaeolei'' consists of 3960 genes comprised from 4,326,849 nucleotides.  The GC content of the bacteria is 57%.  The bacteria's chromosomal topology is circular.  It also consists of two plasmids, pMAQU01 and pMAQU02[http://www.ncbi.nlm.nih.gov/sites/entrez?Db=genome&Cmd=ShowDetailView&TermToSearch=20231].
''Marino aquaeolei'' consists of 3960 genes comprised from 4,326,849 nucleotides.  The GC content of the bacteria is 57%.  The bacteria's chromosomal topology is circular.  It also consists of two plasmids, pMAQU01 and pMAQU02[http://www.ncbi.nlm.nih.gov/sites/entrez?Db=genome&Cmd=ShowDetailView&TermToSearch=20231].  The nucleotides make up one double-stranded circular DNA.


==Cell structure and metabolism==
==Cell structure and metabolism==

Revision as of 03:40, 4 June 2007

A Microbial Biorealm page on the genus Marinobacter aquaeolei

SEM of Marinobacter aquaeolei grown on Halomonas complex medium containing 5% NaCl. From JGI.

Classification

Higher order taxa

Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Alteromonadaceae; Marinobacter; Marinobacter aquaeolei

Species

Marinobacter aquaeolei

NCBI: Taxonomy

Description and significance

The schematic of a bacterium by Todd Groesbeck, NCBI. From NCBI

Marinobacter aquaeolei are Gram-negative, non-spore forming, and rod shaped cells. They are motile by the use of a polar flagellum. Several strains of this cell were found at the head of and oil-producing well on an offshore platform in southern Vietnam. Marinobacter aquaeolei can be found throughout the water column and in the deep ocean. These organisms are capable of extremophilic lifestyles and are moderately halophilic and mesophilic. The growth of cells occurred at NaCl concentrations between 0% - 20%; the optimal level is at 5% NaCl.


Iron is one of the most important redox-active elements in the solar system. Iron oxidation is distributed widely among prokaryotes and the activities of these iron oxidizing bacteria is critical to many major elemental cycles, including the carbon cycle. Sequencing this bacteria can help us understand the fundamental biology of iron oxidation. Marinobacter aquaeolei grows rapidly on plates and in liquid culture and is open to biochemical methods of genetic manipulation. Marinobacter aquaeolei produce a rare class of amphiphilic siderophores that are envisioned to represent a unique mechanism for iron acquisition in marine bacteria.

Genome structure

Describe the size and content of the genome. How many chromosomes? Circular or linear? Other interesting features? What is known about its sequence? Does it have any plasmids? Are they important to the organism's lifestyle?

Marino aquaeolei consists of 3960 genes comprised from 4,326,849 nucleotides. The GC content of the bacteria is 57%. The bacteria's chromosomal topology is circular. It also consists of two plasmids, pMAQU01 and pMAQU02[1]. The nucleotides make up one double-stranded circular DNA.

Cell structure and metabolism

Describe any interesting features and/or cell structures; how it gains energy; what important molecules it produces.

Rod shaped isolates were obtained from offshore oil wells and all were Gram-negative cells. They were able to grow in a wide range of NaCl mediums. They were found to grow singly or in pairs. The cells were actively mobile by a polar flagellum only visible in some areas. "dl-Lactate, acetate, succinate, fumarate, citrate, l-glutamate and butyrate could be used as sole carbon and energy sources..." (Huu NB, Denner EB, Ha DT, Wanner G, Stan-Lotter H. Marinobacter aquaeolei can also use pristane, n-hexadecane, and crude oil components as sole carbon sources.

Ecology

Describe any interactions with other organisms (included eukaryotes), contributions to the environment, effect on environment, etc.

Marinobacter aquaeolei can degrade n-hexadecane, pristane and some crude oil components making it useful for research. Since Iron is so abundant, the oxidation of it by this particular bacteria proves to be something useful for research. Scientists still have a poor understanding of this major metabolic pathway. Marinobacter aquaeolei is important to understand for both biological iron oxidation and iron acquisition in the ocean.

Pathology

How does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.

Application to Biotechnology

Does this organism produce any useful compounds or enzymes? What are they and how are they used?

Current Research

Enter summaries of the most recent research here--at least three required

References

Joint Genome Institute Why Sequence Marinobacter aquaeolei?

Nguyen, B. Huu. 1999. Marinobacter aquaeolei sp. nov., a halophilic bacterium isolated from a Vietnamese oil-producing well.

Marinobacter aquaeolei VT8, complete genome, NCBI.

Edited by student of Rachel Larsen and Kit Pogliano