Matt Brown, Genie Song - B. pumilus

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Template:B. "pumilus"

Classification

  • Kingdom: Bacteria
  • Phylum: Firmicutes
  • Class: Bacilli
  • Order: Bacillales
  • Family: Bacillaceae


Species

NCBI: Taxonomy

  • Genus: Bacillus
  • Species: pumilus

Habitat Information

The soil sample was collected from 6607 Brodie Lane, Austin TX, 78745. January 24th with no rainfall in the previous 24 hours, no solar radiation, 46% humidity, 30.44 inches of seal level pressure and an air temperature of 65 degrees Fahrenheit. The soil consisted of a speck stony clay loam.


Description and Significance

  • Colonial morphology: Irregular, wrinkled and opaque. Colonies are smooth with a yellow tint. Margins are undulated.
  • Cellular morphology: Rod shaped, bacillus, gram positive, and motile.
  • Significance:Shows high resistance to environmental stresses such as: UV radiation, hydrogen peroxide, and desiccation (state of extreme dryness).
*Isolates of B. pumilus were recently recovered aboard the International Space Station from hardware surfaces and air particles.
*Food intoxications to humans and may produce toxins.
*Its plasmids used for gene transfer systems.
*Involved in rectal fistulas.
*Causes widespread lysis and damage to HEp-2 cells.
*Used in agriculture for its antifungal activity as fungicides. Growth of the bacterium on plant roots prevents Rhizoctonia and Fusarium spores from germinating.
*Generally non-pathogenic, only three documented cases of cutaneous infection.

Genome Structure

B. "pumilus" is created from a singular circular chromosome of an estimated 4000 genes and a median protein count of 3721. The most common bases being GC compromising 41.5% of the sequence. The median length of the sequence is 3.7 Mb.

MC1- Forward Sequence: ATTGGGCGTAAGGGCTCGCAGGCGGTTTCTTAAGTCTGATGTGAAAGCCCCCGG

CTCAACCGGGGAGGGTCATTGGAAACTGGGAAACTTGAGTGCAGAAGAGGAGAGTGGAATTCCACGTGTAGCGGTGAAAT GCGTAGAGATGTGGAGGAACACCAGTGGCGAAGGCGACTCTCTGGTCTGTAACTGACGCTGAGGAGCGAAAGCGTGGGGA GCGAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTAGGGGGTTTCCGCCCCTTAGTGC TGCAGCTAACGCATTAAGCACTCCGCCTGGGGAGTACGGTCGCAAGACTGAAACTCAAAGGAATTGACGGGGGCCCGCAC AAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCTCTGACAACCCTAGAGA TAGGGCTTTCCCTTCGGGGACAGAGTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTC CCGCAACGAGCGCAACCCTTGATCTTAGTTGCCAGCATTCAGTTGGGCACTCTAAGGTGACTGCCGGTGACAAACCGGAG GAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTGCTACAATGGACAGAACAAAGGGCT GCAAGACCGCAAGGTTTAGCCAATCCCATAAATCTGTTCTCAGTTCGGATCGCAGTCTGCAACTCGACTGCGTGAAGCTG GAATCGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCACGAG AGTTTGCAACACCCGAAGTCGGTGAGGTAACCTTTATGGAGCCAGCCGCCGAA


Cell Structure, Metabolism and Life Cycle

Interesting features of cell structure; how it gains energy; what important molecules it produces.


Physiology and Pathogenesis

  • Gram Reaction: Gram positive
  • Capsule stain: Negative
  • Endospore stain: Positive
  • Motility results: Motile
  • Phenol Red Broth: No change
  • Starch Hydrolysis: No clearing
  • Casein Hydrolysis: No clearing. Casease is absent. Negative.
  • Gelatin Hydrolysis: Gelatin is solid. No gelatinase is present.
  • DNA Hydrolysis: Clearing in agar around growth. DNAse is present.
  • Lipid Hydrolysis: No clearing in agar. Lipase is not present.
  • Methyl Red: Negative.No color change. No mixed acid fermentation.
  • Voges Proskauer: Negative.
  • Citrate Test: Negative.
  • SIM Tests (3 in 1): Negative
  • Nitrate Reduction Test: Positive for nitrite.
  • Urea Hydrolysis: Negative
  • Triple Sugar Iron Agar: Negative, sulfur was used as terminal electron acceptor, no gas present.
  • PCR: Procedure performed in class
  • Oxidase: Negative.
  • Eosin Methylene Blue Agar: Negative (light pink).
  • Hektoen Enteric Agar: No change visible.
  • MacConkey Agar: Positive, no bile precipitate.
  • Decarboxylation: Lysine: negative; Ornithine and Arginine: positive.
  • Phenylalanine Deaminase: Negative.
  • Catalase: Positive.
  • Blood Agar: Alpha, partial clearing (green).
  • Mannitol Salt Agar: No change.
  • 6.5% NaCl Broth Salt Tolerance Test: Negative.
  • Bile Esculin Test: Negative.


References

[Sample reference] Takai, K., Sugai, A., Itoh, T., and Horikoshi, K. "Palaeococcus ferrophilus gen. nov., sp. nov., a barophilic, hyperthermophilic archaeon from a deep-sea hydrothermal vent chimney". International Journal of Systematic and Evolutionary Microbiology. 2000. Volume 50. p. 489-500.

Author

Page authored by _____, student of Prof. Kristine Hollingsworth at Austin Community College.

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