Matt Brown, Genie Song - B. pumilus
Classification
- Kingdom: Bacteria
- Phylum: Firmicutes
- Class: Bacilli
- Order: Bacillales
- Family: Bacillaceae
Species
NCBI: Taxonomy |
- Genus: Bacillus
- Species: pumilus
Habitat Information
The soil sample was collected from 6607 Brodie Lane, Austin TX, 78745. January 24th with no rainfall in the previous 24 hours, no solar radiation, 46% humidity, 30.44 inches of seal level pressure and an air temperature of 65 degrees Fahrenheit. The soil consisted of a speck stony clay loam.
Description and Significance
- Colonial morphology: Irregular, wrinkled and opaque. Colonies are smooth with a yellow tint. Margins are undulated.
- Cellular morphology: Rod shaped, bacillus, gram positive, and motile.
- Significance:Shows high resistance to environmental stresses such as: UV radiation, hydrogen peroxide, and desiccation (state of extreme dryness).
- Isolates of B. pumilus were recently recovered aboard the International Space Station from hardware surfaces and air particles.
- Food intoxications to humans and may produce toxins.
- Its plasmids used for gene transfer systems.
- Involved in rectal fistulas.
- Causes widespread lysis and damage to HEp-2 cells.
- Used in agriculture for its antifungal activity as fungicides. Growth of the bacterium on plant roots prevents Rhizoctonia and Fusarium spores from germinating.
- Generally non-pathogenic, only three documented cases of cutaneous infection and two septic infections.
Genome Structure
B. "pumilus" is created from a singular circular chromosome of an estimated 4000 genes and a median protein count of 3721. The most common bases being GC compromising 41.5% of the sequence. The median length of the sequence is 3.7 Mb.
- MC1- Forward Sequence: ATTGGGCGTAAGGGCTCGCAGGCGGTTTCTTAAGTCTGATGTGAAAGCCCCCGG
CTCAACCGGGGAGGGTCATTGGAAACTGGGAAACTTGAGTGCAGAAGAGGAGAGTGGAATTCCACGTGTAGCGGTGAAAT GCGTAGAGATGTGGAGGAACACCAGTGGCGAAGGCGACTCTCTGGTCTGTAACTGACGCTGAGGAGCGAAAGCGTGGGGA GCGAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTAGGGGGTTTCCGCCCCTTAGTGC TGCAGCTAACGCATTAAGCACTCCGCCTGGGGAGTACGGTCGCAAGACTGAAACTCAAAGGAATTGACGGGGGCCCGCAC AAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCTCTGACAACCCTAGAGA TAGGGCTTTCCCTTCGGGGACAGAGTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTC CCGCAACGAGCGCAACCCTTGATCTTAGTTGCCAGCATTCAGTTGGGCACTCTAAGGTGACTGCCGGTGACAAACCGGAG GAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTGCTACAATGGACAGAACAAAGGGCT GCAAGACCGCAAGGTTTAGCCAATCCCATAAATCTGTTCTCAGTTCGGATCGCAGTCTGCAACTCGACTGCGTGAAGCTG GAATCGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCACGAG AGTTTGCAACACCCGAAGTCGGTGAGGTAACCTTTATGGAGCCAGCCGCCGAA
Cell Structure, Metabolism and Life Cycle
Interesting features of cell structure; how it gains energy; what important molecules it produces.
Physiology and Pathogenesis
- Gram Reaction: Gram positive
- Capsule stain: Negative
- Endospore stain: Positive
- Motility results: Motile
- Phenol Red Broth: No change
- Starch Hydrolysis: No clearing
- Casein Hydrolysis: No clearing. Casease is absent. Negative.
- Gelatin Hydrolysis: Gelatin is solid. No gelatinase is present.
- DNA Hydrolysis: Clearing in agar around growth. DNAse is present.
- Lipid Hydrolysis: No clearing in agar. Lipase is not present.
- Methyl Red: Negative.No color change. No mixed acid fermentation.
- Voges Proskauer: Negative.
- Citrate Test: Negative.
- SIM Tests (3 in 1): Negative
- Nitrate Reduction Test: Positive for nitrite.
- Urea Hydrolysis: Negative
- Triple Sugar Iron Agar: Negative, sulfur was used as terminal electron acceptor, no gas present.
- PCR: Procedure performed in class
- Oxidase: Negative.
- Eosin Methylene Blue Agar: Negative (light pink).
- Hektoen Enteric Agar: No change visible.
- MacConkey Agar: Positive, no bile precipitate.
- Decarboxylation: Lysine: negative; Ornithine and Arginine: positive.
- Phenylalanine Deaminase: Negative.
- Catalase: Positive.
- Blood Agar: Alpha, partial clearing (green).
- Mannitol Salt Agar: No change.
- 6.5% NaCl Broth Salt Tolerance Test: Negative.
- Bile Esculin Test: Negative.
References
Author
Page authored by Matthew Brown and Eugenie Song, student of Prof. Kristine Hollingsworth at Austin Community College.