Classification -higher order taxa- Phylum: Bacteroidetes, Family: Flavobacteriaceae, Genus: Mesonia because it showed a 97.5% genome in common with Mesonia mobilis and 95.4% in common with Mesonia algae. Mesonia algae is found from green algae and Mesonia mobilis is found from seawater. -Species- Mesonia phycicola
Description and significance This microbe is aerobic, which means it uses oxygen for cellular respiration. It is also a gram-negative so during Gram staining protocol it will not retain the crystal violet dye. The rod shape (bacillus) it has is common among many different taxonomic groups. It is non motile and also a catalase- and oxidase- positive bacterium. Much like the other species in the genus of Mesonia, Mesonia phycicola is isolated from a seawater environment. The species was found in seaweed collected in the vicinity of Mara in Jeju province, Republic of Korea. If experiments are done on this species, they should be grown in a marine broth of 2% sea salts (Sigma) solution. The colonies of this microbe are smooth, circular and convex. A yellow pigment was observed after 5 days of growth in a marine broth at 30 degrees Celsius. Growth can occurred between 10-30 degrees Celsius. The ph for growth is 6.1-9.1 and the NaCl concentration should be between 1-12% NaCl. The major menaquinone of Mesonia phycicola was MK-6. DNA G+C content was much lower than the reported range for the Mesonia genus. It was reported at 30.0 mol% and discovered by HPLC for the Mesonia phycicola species. The size of a typical cell is 0.6-1.6 micrometers in diameter and 2.1-2.5 micrometers in length.
Current Research -Wizard Genomic DNA Purification kit (Promega) isolated and purified chromosomal DNA in the study by Lee (Lee, S.D. 2007). -CLUSTAL_X was used on the species to align sequences multiple times (Thompson et al., 1997). -DNA-DNA hybridization experiments were preformed on the species by labelling the strain with the non-radioactive DIG High Prime System (Wayne et al., 1987).
Kang, H. S., & Lee, S. D. (2010). Mesonia phycicola sp. nov., isolated from seaweed, and emended description of the genus mesonia. International Journal of Systematic and Evolutionary Microbiology, 60(3), 591-594. Retrieved from http://ijs.sgmjournals.org/content/60/3/591.full? sid=e68583bc-c7c9-4dee-bc35-1c4a6126dc91
Lee, S. D. (2007). Tamlana crocina gen. nov., sp. nov., a marine bacterium of the family Flavobacteriaceae, isolated from beach sediment in Korea. Int J Syst Evol Microbiol 57, 764–769.
Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. (1997). The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.
Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors (1987). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.