Difference between revisions of "Methanospirillum hungatei"

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Page authored by Shannon Henderson and David Hall, students of [http://www.kbs.msu.edu/faculty/lennon/ Prof. Jay Lennon] at Michigan State University.
 
Page authored by Shannon Henderson and David Hall, students of [http://www.kbs.msu.edu/faculty/lennon/ Prof. Jay Lennon] at Michigan State University.
  
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Latest revision as of 15:12, 2 October 2017

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Classification

Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales; Methanospirillaceae

Species

NCBI: Taxonomy

Methanospirillum hungatei

Description and Significance

P=plug, S=sheath, W=(cell) wall, AM=amorphous matrix
Image from (Beveridge et. al. 1991)

Methanospirillum hungatei was first found in sewage sludge and was named in honor of R.E. Hungate. This genus and species name was first proposed in 1974 by Ferry et. al. The cultures are usually yellow in color, circular in shape, and convex with lobate margins (Ferry et. al. 1974). The cells are spiral shaped (curved rods) and range from 0.5-7.4 microns in diameter and 15 to several hundred microns long and have tufts of polar flagella that provide a small amount of motility (Ferry et. al. 1974).

The optimum habitat for these organisms has a temperature range of 30-37° Celsius (mesophilic) and a pH range of 6.6-7.4 (Ferry et. al. 1974).

This microorganism is very important to the waste treatment and bioenergy industries because it can break down organic wastes and produces methane in the process.

Genome Structure

Methanospirillum hungatei has circular chromosomes of length 3544738; it has 3139 gene proteins and 66 RNA genes (Kanehisa Labs). The guanine and cytosine content is 45% for this organism (Ferry et. al. 1974).

Cell Structure, Metabolism and Life Cycle

Methanospirillum hungatei has very interesting structure. For instance, it is mostly gram negative, but the multicellular filaments at the ends test as gram positive (Beveridge et. al. 1991). It grows in groups consisting of chains of cells about 9-12 cells long, which are encased in a paracrystalline sheath (Beveridge et. al. 1991). It is thought that this proteinaceous sheath acts as a kind of pressure regulator; the sheath is able to expand as the internal pressure of the cell increases due to the production of methane, which increases the permeability of the sheath to allow methane to diffuse out and hydrogen and carbon dioxide to diffuse in; the study found a possible maximum internal pressure of 300-400 atm (Xu et. al. 1996). It does not form spores (Ferry et. al. 1974). The sheath circles the cell in hoop-like structures that also contain plugs on the surface, which have holes that are 3-6 times larger than the pores on the rest of the sheath, presumably to allow larger particles through (Xu et. al. 1996).

The cell's cytoplasmic membrane consists of 7-8% of the cell's dry weight, while the sheath is about 13% of the cell's dry weight. The cytoplasmic membrane is 35-37% lipids, 45-50% protein, and 10-12% carbohydrates. 90% of the cell's phytanyl lipids are contained in the cytoplasmic membrane. (Sprott et. al. 1983).

The flagella on these organisms are about 10nm long, which is smaller than most bacteria (Southam et. al. 1990). Since groups of 2-3 cells still have some motility, it is thought that there must be some sort of communication method between these cells to coordinate the movement of their flagella (Southam et. al. 1990).

This microorganism is considered a "fastidious anaerobe," meaning it is very sensitive to oxygen (Ferry et. al. 1974). It is also a methanogen and a hydrogenotroph and grows best in an atmosphere with a mixture of hydrogen and carbon dioxide, with 80% H2 and 20% CO2 being the optimum conditions (Ferry et. al. 1974). It can use formate or acetate as a carbon source and has been shown to demonstrate a chemotactic response to the presence of acetate (Migas et. al. 1989). According to a study that used magnetic resonance of 13C to tag carbon dioxide and acetate, the acetate and carbon dioxide are used to form pyruvate, which then goes through the glucogenic pathway to form carbohydrates (Ekiel et. al. 1983). They found that 99% of the carbon in the methane produced comes from carbon dioxide (Ekiel et. al. 1983).

Ecology and Pathogenesis

Methanospirillum hungatei, as well as many other methanogens, are often used to anaerobically treat organic wastes, such as municipal wastewater or industrial waste, and can sometimes be good at degrading particularly complex wastes (Qui et. al. 2004). The degradation of some wastes, such as phtalate isomer-containing wastewaters, is accomplished by syntrophic cooperation between several different kinds of microorganisms, for instance microorganisms that produce hydrogen or formate coupled with Methanospirillum hungatei, which consumes hydrogen and formate (Qui et. al. 2004). Treating waste anaerobically can sometimes be more cost efficient than aerobically because there is no need for aeration, which can use a lot of energy and therefore cost a lot of money.

Methane is a greenhouse gas, but can often be captured from constructed waste treatment systems and used as a fuel in place of natural gas, which is a fossil fuel.

No pathogenesis is known.

References

1: NCBI. “Methanospirillum hungatei.” Taxonomy Browser.

2: Ferry, J.G., Smith, P.H., and Wolfe, R.S. “Methanospirillum, a New Genus of Methanogenic Bacteria,and Characterization of Methanospirillum hungatii sp.nov.” International Journal of Systematic Bacteriology. 1974. Volume 24. p. 465-469.

3: Migas, J., Anderson, K.L., Cruden, D.L., and Markovetz, J. “Chemotaxis in Methanospirillum hungatei.” Applied and Environmental Microbiology. 1989. Volume 55. p. 264-265.

4: Kanehisa Laboratories in the Bioinformatics Center of Kyoto University and the Human Genome Center of the University of Tokyo. “Methanospirillum hungatei.” Kyoto Encyclopedia of Genes and Genomes.

5: Beveridge, T.J., Sprott, G.D., Whippey, P. “Ultrastructure, inferred porosity, and gram-staining character of Methanospirillum hungatei filament termini describe a unique cell permeability for this archaeobacterium.” Journal of Bacteriology. 1991. Volume 173. p. 130-140. PMCID: PMC207166.

6: Xu, W., Mulhern, P.J., Blackford, B.L., Jericho, M.H., Firtel, M., Beveridge, J. "Modeling and Measuring the Elastic Properties of an Archaeal Surface, the Sheath of Methanospirillum hungatei, and the Implication for Methane Production." Journal of Bacteriology. 1996. Volume 178. p. 3106-3112.

7: Southam, G., Firtel, M., Blackford, B.L., Jericho, M.H., Xu, W., Mulhern, P.J., Beveridge, T.J. "Transmission Electron Microscopy, Scanning Tunneling Microscopy, and Atomic Force Microscopy of the Cell Envelope Layers of the Archaeobacterium Methanospirillum hungatei GP1." Journal of Bacteriology. 1993. Volume 175. p. 1946-1955.

8: Southam, G., Kalmokoff, M.L., Jarrell, K.F., Koval, S.F., Beveridge, T.J. "Isolation, Characterization, and Cellular Insertion of the Flagella from Two Strains of the Archaebacterium Methanospirillum hungatei." Journal of Bacteriology. 1990. Volume 172. p. 3221-3228.

9: Sprott, G.D., Shaw, K.M., Jarrell, K.F. "Isolation and Chemical Composition of the Cytoplasmic Membrane of the Archaebacterium Methanospirillum hungatei." Journal of Biological Chemistry. 1983. Volume 258. p. 4026-4031.

10: Muller, N., Schleheck, D., Schink, B. "Involvement of NADH:Acceptor Oxidoreductase and Butyryl Coenzyme A Dehydrogenase in Reversed Electron Transport during Syntrophic Butyrate Oxidation by Syntrophomonas wolfei." Journal of Bacteriology. 2009. Volume 191. p. 6167-6177.

11: Ekiel, I., Smith, I.P.C., Sprott, G.D. "Biosynthetic Pathways in Methanospirillum hungatei as Determined by 13C Nuclear Magnetic Resonance." Journal of Bacteriology. 1983. Volume 156. p. 316-326.

12: Qui, Y., Sekiguchi, Y., Imachi, H., Kamagata, Y., Tseng, I., Cheng, S., Ohashi, A., Harada, H. "Identification and Isolation of Anaerobic, Syntrophic Phthalate Isomer-Degrading Microbes from Methanogenic Sludges Treating Wastewater from Terephthalate Manufacturing." Aplied and Environmental Microbiology. 2004. Volume 70. p. 1617-1626.

Author

Page authored by Shannon Henderson and David Hall, students of Prof. Jay Lennon at Michigan State University.