Mycoplasma pulmonis: Difference between revisions

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4.Chambaud, Isabelle, Roland Heilig, Stéphane Ferris, Valérie Barbe, and Delphine Samson. "The Complete Genome Sequence of the Murine Respiratory Pathogen Mycoplasma Pulmonis." PubMedCentral os 29.10 (2001): 2145-2153. PubMed. 2 May 2007 <http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=55444>.
4.Chambaud, Isabelle, Roland Heilig, Stéphane Ferris, Valérie Barbe, and Delphine Samson. "The Complete Genome Sequence of the Murine Respiratory Pathogen Mycoplasma Pulmonis." PubMedCentral os 29.10 (2001): 2145-2153. PubMed. 2 May 2007 <http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=55444>.


5.Minion, F C., and J D. Goguen. "Trypsin-Sensitive, Bovine Serum Albumin-Dependent Hemolysis Activity in Mycoplasma Pulmonis." Infection and Immunity 49 (1985): 440-442. PubMed Central. 4 June 2007 <http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=262037>.


Edited by student of [mailto:ralarsen@ucsd.edu Rachel Larsen] and Kit Pogliano
 
 
Edited by Grace Wanto

Revision as of 02:04, 5 June 2007

A Microbial Biorealm page on the genus Mycoplasma pulmonis

Mycoplasma pulmonis. The white bar equals 1mm. Courtesy of mycoplasma-experience

Classification

Micoplasma2.gif

Higher order taxa

Bacteria; Firmicutes; Mollicutes; Mycoplasmataceae; Mycoplasma

Species

Mycoplasma pulmonis

Description and significance

The organism falls under genus mycoplasma that are easily distinguished from other bacteria due to its small size (0.3-0.8 micron in diameter) and lack of cell wall. Its cell membrane is rich in protein components that consists of structurally adaptive lipoproteins used to invade host immune system, attachment to the host cells, and pathogenic invasion. The primary habitats of human and animal mycoplasma are in mucous membranes of the respiratory and urogenital tracts, eyes, mammary glands and the joints.

Genome structure

Genome.png

Mycoplasma pulmonis are among the first organism to have its genome sequenced due to its small size. Mycoplasmas has the smallest of the prokaryotic genome and it lack complex gene-regulatory systems. M.pulmonis has a single circular 963 879 bp chromosome with a G + C content of 26.6 mol%. The genome contains 782 putative coding sequences (CDSs)with 482 CDS that could havefunction assigned and 92 CDS that have sequence matched to hypothetical protein. The genome also have a single set of rRNA genes and 29 tRNAs genes.

The length of the tandem repeat region of the variable surface antigen (Vsa) protein determines the biofilm formation of M.pulmonis. When Vsa protein are short with few tandem repeats, they form biofilm that attaches the polystyrene and glass. However, when Vsa protein are long with many tandem repeats, M. pulmonis are found floating on the medium as microcolonies. Surprisingly, several of the genes previously reported to be essential for a self-replicating minimal cell are missing in the M.pulmonis genome although this one is larger than the other mycoplasma genomes fully sequenced until now

Cell structure and metabolism

The majority of mycoplasmas are deficient in genes coding for components of intermediary and energy metabolism and thus generates ATP using electron transport phosphorylation or glycolysis. They lost most of their genes involved in amino acid and cofactor biosynthesis, synthesis of the cell wall, lipid metabolism, and cellular processes during evolution. Therefore they serves as model for minimal structure needed for cell metabolism. The bacteria

Pathology

Mycoplasma pulmonis is the agent of murine respiratory mycoplasmosis (MRM), a pneumonia-like slow progressing disease that sometimes does not develop clinical symptoms. It can also cause genital infections which are associated with a decreased fertility both in rodent.

The predicted virulence factor for M.pulmonis are hemolysin, secreted nucleases and a glyco-protease. They do not lyse the erythrocytes but instead they rapidly lyse it in erythrocytes with cytoskeletal defficiency because it increases diffusion for glycophorin. Trypsin on the cell wall of M.pulmonis plays part in hemaglutination of the red blood cells (RBCs) because it cleaves the glycophorin on the membrane of RBCs. Bovin serum albumin also thought to assist in the hemaglutination of the erythrocytes.

The species is also capable of gliding motility in which the mechanism is still unknown. However, it is hypothesized that they glide over the surface by employing actin-like cytoskeletal components and motility proteins specific for the microbial group.

Application to Biotechnology

Complete genome sequence of the organism enables it to serve as the model organism for bacterial evolution related to pathogenicity. It is also useful as model for studying mycoplasmal infections.

Current Research

1. Evidence for type III restriction and modification systems in Mycoplasma pulmonis.

  J Bacteriol. 2007 Mar;189(6):2197-202. Epub 2007 Jan 5.
  Mycoplasma pulmonis is predicted to code for type III restriction and modification (R-M) enzymes.

2. A stochastic mechanism for biofilm formation by Mycoplasma pulmonis.

  J Bacteriol. 2007 Mar;189(5):1905-13. Epub 2006 Dec 1. 
  Biofilm formation of M. pulmonis depends on  the length of Vsc protein.

3. Neutrophil histamine contributes to inflammation in mycoplasma pneumonia.

  J Exp Med. 2006 Dec 25;203(13):2907-17. Epub 2006 Dec 11.  
  Mycoplasma directly stimulated histamine production by naive neutrophils and strongly upregulated mRNA encoding histidine decarboxylase, the rate-limiting enzyme in histamine synthesis.

References

1.Bischoff, Joe, Mikhail Domrachev, Scott Federhen, Carol Hotton, Detlef Leipe, Vladimir Soussov, Richard Sternberg, and Sean Turner. "Mycoplasma Pulmonis." NCBI. 1 May 2007 <http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=2107&lvl=3&p=mapview&p=has_linkout&p=blast_url&p=genome_blast&keep1&srchmode=1&unlock&lin=s

2.Wl, Simmons, Bolland Jr, Daubenspeck Jm, and Dybviq K. "A Stochastic Mechanism for Biofilm Formation by Mycoplasma Pulmonis." os. PubMed. 2 May 2007 <http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&dopt=AbstractPlus&list_uids=17142389&query_hl=5&itool=pubmed_docsum>

3."Mycoplasma Pulmonis UAB CTIP Project At Genoscope." NCBI. 2 May 2007 <http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=genomeprj&cmd=Retrieve&dopt=Overview&list_uids=100>.

4.Chambaud, Isabelle, Roland Heilig, Stéphane Ferris, Valérie Barbe, and Delphine Samson. "The Complete Genome Sequence of the Murine Respiratory Pathogen Mycoplasma Pulmonis." PubMedCentral os 29.10 (2001): 2145-2153. PubMed. 2 May 2007 <http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=55444>.

5.Minion, F C., and J D. Goguen. "Trypsin-Sensitive, Bovine Serum Albumin-Dependent Hemolysis Activity in Mycoplasma Pulmonis." Infection and Immunity 49 (1985): 440-442. PubMed Central. 4 June 2007 <http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=262037>.


Edited by Grace Wanto