Natronomonas Pharaonis: Difference between revisions

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Edited by Sarah Kwan, student of Rachel Larsen and Kit Pogliano at UCSD
 


===Species===
===Species===




Natronomonas Pharaonis
<i>Natronomonas pharaonis</i>




Edited by Sarah Kwan, student of Rachel Larsen and Kit Pogliano at UCSD.
==Description and significance==
 
<i> Natronomonas pharaonis </i> is an aerobic, haloalkaliphilic archaeon that is sensitive to high concentrations of magnesium, but grows the best in conditions of 3.5 M NaCl and at a pH of 8.5.  The first isolated strains of <i> Natronomonas pharaonis </i> were found in the soda lakes of Egypt and Kenya.  These soda lakes are highly saturated with salt and have a pH of 11.  Genome analysis shows adaptation strategies of alkaliphiles in regards to its respiratory chain, nitrogen metabolism, and its cell envelope.


==Description and significance==
Strains of Natronomonas pharaonis were first isolated in Egypt and Kenya from highly saline soda lakes with a pH of 11.  N. pharaonis is an aerobic, haloalkaliphilic archaeon that grows optimally in 3.5 M NaCl and at a pH of 8.5, but it is sensitive to high magnesium concentrations.  Genome analysis shows adaptation strategies of alkaliphiles in regards to its respiratory chain, nitrogen metabolism, and its cell envelope.


==Genome structure==
==Genome structure==
Describe the size and content of the genomeHow many chromosomes? Circular or linear?  Other interesting features?  What is known about its sequence?
 
Does it have any plasmids?  Are they important to the organism's lifestyle?
The genome of ''N. pharaonis'' consists of three circular replicons, one 2.6-Mb chromosome and two plasmids (PL131 - 131kb, PL23 - 23kb).  Its chromosome is GC-rich (63.4% GC) with an integrated copy of PL23''N. pharaonis'' also contains a high amount of acidic amino acids (average 19.3%) in its cytoplasmic proteins. This results in low isoelectric points (average pI 4.6), which is an adaptive feature of haloarchaea in order to survive in its hypersaline environment.
 
 


==Cell structure and metabolism==
==Cell structure and metabolism==
Describe any interesting features and/or cell structures; how it gains energy; what important molecules it produces.


==Ecology==
''Natronomonas pharaonis'' has a high degree of nutritional self-sufficiency.  It contains the correct enzymes for the biosynthesis of amino acids and coenzymes.  However, because it lacks key enzymes of glycolytic pathways, ''N. pharaonis'' will most likely not be able to use sugar.  The genome analysis shows that ''N. pharaonis'' supplies ammonia through three main mechanisms, allowing them to survive in extreme pH conditions, which reduce levels of ammonium ions.
Describe any interactions with other organisms (included eukaryotes), contributions to the environment, effect on environment, etc.


==Pathology==
How does this organism cause disease?  Human, animal, plant hosts?  Virulence factors, as well as patient symptoms.


==Application to Biotechnology==
Does this organism produce any useful compounds or enzymes?  What are they and how are they used?


==Current Research==
==Current Research==


Enter summaries of the most recent research here--at least three required
"Genome-wide proteomics of Natronomonas pharaonis" January 2007.  A shotgun sequencing approach and SDS-PAGE was used to reach further conclusions about ''N. pharaonis'''s cellular physiology.  A set of identified proteins consist of about 60% of the cytosolic proteins involved in metabolism and genetic information processing.  Among the halophilic archaea, many of these proteins show a high genetic variablility.
 
"Large-Scale Identification of N-Terminal Peptides in the Halophilic Archaea Halobacterium salinarum and Natronomonas pharaonis" April 2007.  A combined fractional diagonal chromatography (COFRADIC) and a strong cation exchange chromatography (SCX)were two methods used to characterize the proteins of N-terminal peptides.  This data allowed for the correct assignment of start codons as well as showing that 60% of proteins undergo  methionine cleavage, instead of 13-18%.
 


==References==
==References==
Falb, M., Pfeiffer, F., Palm, P., Rodewald, K., Hickmann, V., Tittor, J., and Oesterhelt, D. "Living with two extremes: Conclusions from the genome sequence of Natronomonas pharaonis." Genome Research. 2005. 15. p. 1336-1343. (http://www.genome.org/cgi/reprint/15/10/1336)
Falb, M., Pfeiffer, F., Palm, P., Rodewald, K., Hickmann, V., Tittor, J., and Oesterhelt, D. "Living with two extremes: Conclusions from the genome sequence of Natronomonas pharaonis." Genome Research. 2005. 15. p. 1336-1343. (http://www.genome.org/cgi/reprint/15/10/1336)
Oesterhelt, D. "Natronomonas pharaonis - overview." Max Planck Institute of Biochemistry. 2007. (http://www.biochem.mpg.de/en/research/rd/oesterhelt/web_page_list/Org_Napha/index.html)


Edited by student of [mailto:ralarsen@ucsd.edu Rachel Larsen] and Kit Pogliano
Edited by student of [mailto:ralarsen@ucsd.edu Rachel Larsen] and Kit Pogliano
KMG

Latest revision as of 18:59, 19 August 2010

This student page has not been curated.

A Microbial Biorealm page on the genus Natronomonas Pharaonis

Classification

Higher order taxa

Archaea; Euryarchaeota; Halobacteria; Halobacteriales; Halobacteriaceae; Natronomonas



Species

Natronomonas pharaonis


Description and significance

Natronomonas pharaonis is an aerobic, haloalkaliphilic archaeon that is sensitive to high concentrations of magnesium, but grows the best in conditions of 3.5 M NaCl and at a pH of 8.5. The first isolated strains of Natronomonas pharaonis were found in the soda lakes of Egypt and Kenya. These soda lakes are highly saturated with salt and have a pH of 11. Genome analysis shows adaptation strategies of alkaliphiles in regards to its respiratory chain, nitrogen metabolism, and its cell envelope.


Genome structure

The genome of N. pharaonis consists of three circular replicons, one 2.6-Mb chromosome and two plasmids (PL131 - 131kb, PL23 - 23kb). Its chromosome is GC-rich (63.4% GC) with an integrated copy of PL23. N. pharaonis also contains a high amount of acidic amino acids (average 19.3%) in its cytoplasmic proteins. This results in low isoelectric points (average pI 4.6), which is an adaptive feature of haloarchaea in order to survive in its hypersaline environment.


Cell structure and metabolism

Natronomonas pharaonis has a high degree of nutritional self-sufficiency. It contains the correct enzymes for the biosynthesis of amino acids and coenzymes. However, because it lacks key enzymes of glycolytic pathways, N. pharaonis will most likely not be able to use sugar. The genome analysis shows that N. pharaonis supplies ammonia through three main mechanisms, allowing them to survive in extreme pH conditions, which reduce levels of ammonium ions.


Current Research

"Genome-wide proteomics of Natronomonas pharaonis" January 2007. A shotgun sequencing approach and SDS-PAGE was used to reach further conclusions about N. pharaonis's cellular physiology. A set of identified proteins consist of about 60% of the cytosolic proteins involved in metabolism and genetic information processing. Among the halophilic archaea, many of these proteins show a high genetic variablility.

"Large-Scale Identification of N-Terminal Peptides in the Halophilic Archaea Halobacterium salinarum and Natronomonas pharaonis" April 2007. A combined fractional diagonal chromatography (COFRADIC) and a strong cation exchange chromatography (SCX)were two methods used to characterize the proteins of N-terminal peptides. This data allowed for the correct assignment of start codons as well as showing that 60% of proteins undergo methionine cleavage, instead of 13-18%.


References

Falb, M., Pfeiffer, F., Palm, P., Rodewald, K., Hickmann, V., Tittor, J., and Oesterhelt, D. "Living with two extremes: Conclusions from the genome sequence of Natronomonas pharaonis." Genome Research. 2005. 15. p. 1336-1343. (http://www.genome.org/cgi/reprint/15/10/1336)

Oesterhelt, D. "Natronomonas pharaonis - overview." Max Planck Institute of Biochemistry. 2007. (http://www.biochem.mpg.de/en/research/rd/oesterhelt/web_page_list/Org_Napha/index.html)

Edited by student of Rachel Larsen and Kit Pogliano

KMG