Nitrobacter hamburgensis

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A Microbial Biorealm page on the genus Nitrobacter hamburgensis

Classification

Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales; Bradyrhizobiaceae; Nitrobacter; Nitrobacter hamburgensis

Description and significance

Nitrobacter hamburgensis, gram negative bacteria, was isolated from soil of the Old Botanic Garden in Hamburg and of a corn field in Yucatan. The main types of environments they inhabit are soil, building sandstone, and sewage sludge. Its cells are 0.5-0.8 x 1.2-2.0 m in size. They are pleomorphic; mostly pear-shaped and motile via one subpolar to lateral flagellum. Intracytoplasmic membranes appear as caps of flattened vesicles or membrane vesicles in the central region of the cell. The bacteria have an enzyme capable of oxidizing nitrite. This is why it is important to sequence the genome of N. hamburgensis.

Genome structure

There is one circular DNA chromosome and three circular DNA plasmids. The chromosome has 4,406,967 nucleotides. Plasmid 1 has 294,829 nucleotides, 2 has 188,318 nucleotides, and 3 has 121,408 nucleotides.

Cell structure and metabolism

N. hamburgensis gains energy from oxidation of nitrite to nitrate via the enzyme nitrite oxidoreductase (NOR). It grows best mixotrophically with a doubling time of 10 hours to 18 hours. Its growth rate under heterotrophic conditions is slower than under mixotrophic conditions, but higher than under lithoautotrophic conditions.

Ecology

Describe any interactions with other organisms (included eukaryotes), contributions to the environment, effect on environment, etc.

Pathology

How does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.

Application to Biotechnology

This organism produces nitrite oxidoreductase used for oxidizing nitrite to nitrate. From the redox reaction; the organism gains energy.

Current Research

Enter summaries of the most recent research here--at least three required

References

Jens Aamand, Thomas Ahl, and Eva Spieck. 1996. "Monoclonal Antibodies Recognizing Nitirite Oxidoreductase fo Nitrobacter hamburgensis, N. winogradskyi, and N. vulgaris." Applied and Environmental Microbiology, vol. 67, no. 7. (2352-5)

E. Bock et al. 1983. “New facultative lithoautotrophic nitrite-oxidizing bacteria.” Archives of Microbiology, vol. 136, no.4. (281-284)

Franco-Rivera A, Paniaqua-Michel S, Zamora-Castro J. 2007. “Characterization and performance of constructed nitrifying biofilms during nitrogen bioremediation of a wastewater effluent.” Journal of industrial microbiology and biotechnology, vol. 34, no. 4. (279-287)

De la Maza, Luis M., Marie T. Pezzlo, and Janet T. Shigei. Color Atlas of Medical Bacteriology. Washington, DC: American Society for Microbiology, 2004.


Edited by Rashonda Butler student of Rachel Larsen and Kit Pogliano