Oceanobacillus iheyensis

A Microbial Biorealm page on the genus Oceanobacillus iheyensis

Classification

Higher order taxa

Bacteria; Firmicutes; Bacillales; Oceanobacillus

Species

iheyensis HTE831

Genus species Oceanobacillus iheyensis HTE831

Description and significance

Oceanobacillus iheyensis HTE831 is an extremely halotolerant and alkaliphilic Bacillus related species. It is isolated from deep sea sediment collected at a depth of 1050m on the Iheya Ridge.(1) The strain is aerobic, Gram positive, rod-shaped, and spore forming. It is motile by the use of meretricious flagella. Isolate HTE831 grows from a pH range of 6.5-9.5 and in 0-21% NaCl. The salinities were 0-21% in NaCl at pH 7.5 and 0-18% NaCl at pH 9.5. The proposal that O.iheyensis is a member of a new species in a new genus came after phylogenetic analysis using 16S rDNA sequencing, chemotaxonomy, and the physiology of strain HTE831. (1)

Genome structure

The genome of O.iheyensis is a single circular chromosome consisting of 3,630,528 nt. The entire genome sequence for O.iheyensis has been determined in Japan MSTC sequencing center. There are 3594 genes and 3500 are protein coding with 92 structural RNAs. The genome has 3496 ORFs with 1972 assigned functions. (1) Six new kind of insertion sequences (Iss), IS667 to IS672, a group II intron (Oi.Int), and an incomplete transponson (Tn8521oi) were identified in O.iheyensis. All the new insertion sequences except IS699 made a 4 to 8 bp duplication of the target site sequence. (5) The ISs carried 23 to 28 bp inverted repeats. Most of the ISs and the group II intron are widely distributed throughout the genome and were inserted in noncoding regions. In HTE831 genome there are 19 identified ISs. Seven of these were truncated, showing the occurrence of internal genome rearrangement. (5)

Cell structure and metabolism

Describe any interesting features and/or cell structures; how it gains energy; what important molecules it produces.

Ecology

Describe any interactions with other organisms (included eukaryotes), contributions to the environment, effect on environment, etc.

Pathology

How does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.

Application to Biotechnology

Does this organism produce any useful compounds or enzymes? What are they and how are they used?

Current Research

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References

(1)Oceanobacillus iheyensis gen. nov., sp. nov., a deep-sea extremely halotolerant and alkaliphilic species isolated from a depth of 1050 m on the Iheya Ridge.Lu J, Nogi Y, Takami H.Microbial Genome Research Group, DEEPSTAR, Japan Marine Science and Technology Center, 2-15 Natsushima, 237-0061, Yokosuka, Japan.

(2)Hideto Takami,a Yoshihiro Takaki, and Ikuo Uchiyama1 Japan Marine Science and Technology Center, Microbial Genome Research Group, 2–15 Natsushima, Yokosuka, Kanagawa 237-0061, Japan and 1Research Center for Computational Science, Okazaki National Research Institute, Nishigonaka 38, Myodaiji, Okazaki, Aichi 444-85

(3)Takami H., Inoue,A., Fuji,F. and Horikoshi,K. (1997) Microbial flora in the deepest sea mud of Mariana Trench. FEMS Microbiol. Lett., 152:, 279–285. [PubMed].

(4)Takami H., Kobata,K., Nagahama,T., Kobayashi,H., Inoue,A. and Horikoshi,K. (1999) Biodiversity in deep-sea sites near the south part of Japan. Extremophiles, 3:, 97–102. [PubMed].

(5)Priest F.G. (1993) Systematics and ecology of Bacillus. In Sonenshein,A.L., Hoch,J.A. and Losick,R. (eds), Bacillus subtilis and Other Gram-Positive Bacteria. ASM Press, Washington, DC, pp. 3–16.

(6)Takami H. (1999) Isolation and characterization of microorganisms from deep-sea mud. In Horikoshi,K. and Tsujii,K. (eds), Extremophiles in Deep-Sea Environments. Springer-Verlag, Tokyo, Japan, pp. 3–26.

(7) Akira Funahashi<funa@symbio.jst.go.jp> Wed Jun 16 22:52:26 JST 2004Oceanobacillus iheyensis. Glycolysis / Gluconeogenesis, [SBML: Level-1 ver-2| Level-2 ver-1 ], [view in KEGG]. Citrate cycle (TCA cycle), [SBML: Level-1 ... sbserv.symbio.jst.go.jp/001/kegg/oih.html

(8) Deep-sea Microorganism Research Group, Japan Marine Science and Technology Center, Yokoruka, Japan. takamih@jamstec.go.jp

(9) Ventosa, A. and Nieto, J.J.: Biotechnological applications and potentialities of halophilic microorganisms. World J. Microbiol.Biotechnol., 11, 85-94 (1995).

(10)www.ebi.ac.uk/2can/genomes/bacteria/Oceanobacillus_iheyensis.html - 9k -

(11) Anthony E. Choudhry,1 Tracy L. Mandichak,1† John P. Broskey,1 Richard W. Egolf,1† Cynthia Kinsland,2 Tadhg P. Begley,2 Mark A. Seefeld,1 Thomas W. Ku,1,James R. Brown,1* Magdalena Zalacain,1 and Kapila Ratnam1*Microbial, Musculoskeletal and Proliferative Diseases and Bioinformatics, GlaxoSmithKline Pharmaceuticals, Collegeville, Pennsylvania 19426,1 and Department of Chemistry and Chemical Biology, Cornell University, Ithaca New York 148502 Received 22 November 2002/Returned for modification 20 January 2003/Accepted 28 February 2003

(12) 625 Oceanobacillus iheyensis PIRSF002583 300 PubMed ID=>12235376

(13)Genome sequence of Oceanobacillus iheyensis isolated from the Iheya Ridge and its unexpected adaptive capabilities to extreme environments. [Nucleic Acids Res. 2002] PMID: 12235376

(14)Robert L. J. Graham, Catherine E. Pollock, S. Naomi O'Loughlin, Nigel G. Ternan, D. Brent Weatherly, Rick L. Tarleton, Geoff McMullan. (2007) Multidimensional analysis of the insoluble sub-proteome ofOceanobacillus iheyensis HTE831, an alkaliphilic and halotolerant deep-sea bacterium isolated from the Iheya ridge. PROTEOMICS 7:1, 82

Edited by student Gloria Slusher