Oxalobacter formigenes: Difference between revisions

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If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.<br><br>
If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.<br><br>


The habitat of this organism is the large intestine of the human gut, and although it is not initially found in the gut at birth, O. formigenes quickly is established in the gut microflora [Mittal]. O. formigenes plays a unique role in the gut microflora in that it is capable of metabolizing 70 to 100 mg per day of oxalate that is ingested in one’s diet [Mittal]. Although soluble oxalate is absorbed in the human small intestine, O. formigenes fulfills the role of metabolizing the remaining dietary oxalate, which can otherwise accumulate, leading to hyperoxaluria and the formation of calcium oxalate stones, which are harmful to the human host [Siva]. This can be considered a symbiosis, in that the organism, a strict anaerobe, is given a safe environment to live and reproduce, along with a steady supply of oxalate to consume. In addition, O. formigenes confers a benefit to its human host. O. formigenes is not explicitly pathogenic.
The habitat of this organism is the large intestine of the human gut, and although it is not initially found in the gut at birth, O. formigenes quickly is established in the gut microflora [Mittal]. O. formigenes plays a unique role in the gut microflora in that it is capable of metabolizing 70 to 100 mg per day of oxalate. [Mittal]. Although soluble dietary oxalate is absorbed in the human small intestine, O. formigenes fulfills the role of metabolizing the remaining dietary oxalate and oxalate produced by the host's metabolism, which can otherwise accumulate, leading to hyperoxaluria and the formation of calcium oxalate stones, which are harmful to the human host [Siva]. This can be considered a symbiosis, in that the organism, a strict anaerobe, is given a safe environment to live and reproduce, along with a steady supply of oxalate to consume. In addition, O. formigenes confers a benefit to its human host in the sense that it degrades the otherwise toxic excess oxalate in the large intestine. O. formigenes is not explicitly pathogenic.


==References==
==References==

Revision as of 18:39, 14 April 2009

Classification

Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Oxalobacteraceae; Oxalobacter

Species

Oxalobacter formigenes

Description and Significance

Oxalobacter formigenes is a nonmotile, non-spore-forming, gram negative beta-proteobacterium that colonizes the human large intestine (colon) [stewart] This organism has also been isolated from the gut of ruminant and other non-ruminant herbivores [Kuhner]. O. formigenes is a strict anaerobe and is thought to be largely important in the metabolism of oxalate in the large intestine that may be remaining from the diet or a byproduct of host metabolism [stewart]. The absence of O. formigenes is thought to be a cause of hyperoxaluria and the formation of calcium oxalate stones in the kidney [Siva], and it is thought that O. formigenes may be useful as a probiotic agent for the treatment of these conditions [stewart].

Genome Structure

Two separate shotgun sequencing projects are currently under way to sequence the genome of O. formigenes, Neither of these projects are complete (and they each have many unresolved contigs). The length of genetic material currently sequenced is approximately 2.4 MB for both projects, and the GC content of this sequence approximately 50%. The currently available sequence likely does not encompass the entire genome due to toxic or unstable (unable to be cloned) portions of the genome. The genome has not yet been annotated, and the number of chromosomes and characteristics of the genome (such as circularity or linearity) are unknown.

[INSERT REFERENCE]

Cell Structure, Metabolism and Life Cycle

Oxalobacter formigenes is a rod-shaped, gram negative obligate anaerobe. This organism requires oxalate as a source of energy in order to survive [Mittal]. O. formigenes requires oxalate as both a source of carbon and energy [Stewart]. [Insert Paragraph A] Additionally, it requires acetate as a carbon source, but has no glycolytic pathways, and is thus unable to grow on sugars [Stewart] O. formigenes utilizes an oxalate/formate antiporter system to generate a proton motive force for the production of ATP (energy) [Stewart]. The exchanged oxalate (taken in by the cell) can then be decarboxylated to regenerate formate for export, or further metabolized for the production of glycerate (See Figure 1) [Stewart]. [Insert Paragraph B].


Figure 1: Oxalate metabolism by Oxalobacter formigenes [Stewart]

Oxalate degradation.jpg

Ecology and Pathogenesis

Habitat; symbiosis; biogeochemical significance; contributions to environment.
If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.

The habitat of this organism is the large intestine of the human gut, and although it is not initially found in the gut at birth, O. formigenes quickly is established in the gut microflora [Mittal]. O. formigenes plays a unique role in the gut microflora in that it is capable of metabolizing 70 to 100 mg per day of oxalate. [Mittal]. Although soluble dietary oxalate is absorbed in the human small intestine, O. formigenes fulfills the role of metabolizing the remaining dietary oxalate and oxalate produced by the host's metabolism, which can otherwise accumulate, leading to hyperoxaluria and the formation of calcium oxalate stones, which are harmful to the human host [Siva]. This can be considered a symbiosis, in that the organism, a strict anaerobe, is given a safe environment to live and reproduce, along with a steady supply of oxalate to consume. In addition, O. formigenes confers a benefit to its human host in the sense that it degrades the otherwise toxic excess oxalate in the large intestine. O. formigenes is not explicitly pathogenic.

References

[Sample reference] Takai, K., Sugai, A., Itoh, T., and Horikoshi, K. "Palaeococcus ferrophilus gen. nov., sp. nov., a barophilic, hyperthermophilic archaeon from a deep-sea hydrothermal vent chimney". International Journal of Systematic and Evolutionary Microbiology. 2000. Volume 50. p. 489-500.

2. Stewart, C.S., Duncan, S. H., Cave, D.R. 2004. "Oxalobacter formigenes and its role in oxalate metabolism in the human gut." FEMS Microbiology Letters. 230: 1-7.

3. Siva, S., Barrack, E.R., Reddy, G.P.V., Thamilselvan, V., Thamilselvan, S., Menon, M., Bhandari, M. 2009. "A Critical analysis of the role of gut Oxalobacter formigenes in oxalate stone disease." BJU International. 103 (1): 18-21.

4. Mittal, R.D, Kumar, R. 2004. "Gut-inhabiting bacterium Oxalobacter formigenes: Role in calcium oxalate urolithiasis." Jorunal of Endourology. 18 (5): 418-424

Author

Page authored by Mark Charbonneau and Ashley Behan, students of Prof. Jay Lennon at Michigan State University.