Paracoccus denitrificans: Difference between revisions

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==Cell structure and metabolism==
==Cell structure and metabolism==
The cell structures of Paracoccus denitrificans is similar to those in an eukaryotic mitochondria.  They are able to obtain energy from both organic, such as methanol and methylamine, and inorganic compounds, such as hydrogen and sulfur.  A unique feature of this bacteria is its ability to single-handedly convert ammonia to dinitrogen.  It oxidizes ammonia to nitrite in a process called heterotrophic nitrification.  Denitrification converts the nitrite product into dinitrogen. 
Describe any interesting features and/or cell structures; how it gains energy; what important molecules it produces.
Describe any interesting features and/or cell structures; how it gains energy; what important molecules it produces.



Revision as of 06:13, 1 June 2007

A Microbial Biorealm page on the genus Paracoccus denitrificans

Classification

Higher order taxa

Bacteria; Proteobacteria; Alpha Proteobacteria; Rhodobacterales; Rhodobacteraceae;

Species

NCBI: Taxonomy

Paracoccus denitrificans

Description and significance

Paracoccus denitrificans is a coccoid shaped gram negative bacteria. They live in the soil in either aerobic or anaerobic environments. They also have the ability to live in many different kinds of media including C1 and sulfur. They can either use organic energy sources, such as methanol or methylamine, or act as chemolithotrophs, using inorganic energy sources with carbon dioxide as their carbon source. Paracoccus denitrificans was first isolate in 1910 by Martinus Beijerinck, a Dutch microbiologist, and was given the name, Micrococcus denitrificans. In 1969, D.H. Davis changed the name of the bacteria to its present name because of the discovery that the bacteria contained many features known to be in mitochonria. It is possible that Paracoccus denitrificans is an ancestor to the eukaryotic mitochondria.


Include a picture or two (with sources) if you can find them.

Genome structure

The genome of Paracoccus denitrificans consists of two circular chromosomes and one plasmid. The first chromosome has 2,852,282 base pairs. The second chromosome has 1,730,097 base pairs and the plasmid has 653,815 base pairs. The plasmid encodes 611 known proteins such as Formyltetrahydrofolate deformylase and TonB-dependent siderophore receptor precursor. These proteins are not essential for the survival of the bacterium; however, the proteins transcribed and translated from the plasmid allow the bacterium to perform many of its metabolic functions.

Cell structure and metabolism

The cell structures of Paracoccus denitrificans is similar to those in an eukaryotic mitochondria. They are able to obtain energy from both organic, such as methanol and methylamine, and inorganic compounds, such as hydrogen and sulfur. A unique feature of this bacteria is its ability to single-handedly convert ammonia to dinitrogen. It oxidizes ammonia to nitrite in a process called heterotrophic nitrification. Denitrification converts the nitrite product into dinitrogen.


Describe any interesting features and/or cell structures; how it gains energy; what important molecules it produces.

Ecology

Paracoccus denitrificans produce nitric oxide and nitrous oxide, which gives rise to atmospheric damage. They are also responsible for the loss of nitrogen fertilizer in agricultural soil.

Describe any interactions with other organisms (included eukaryotes), contributions to the environment, effect on environment, etc.

Pathology

There are no known pathological effects of this bacterium on humans.

Application to Biotechnology

Does this organism produce any useful compounds or enzymes? What are they and how are they used?

Current Research

A research study conducted in 2007 looked at the complex formed between cytochrome c oxidase. The lab used multi-frequency pulse electron paramagnetic resonance spectroscopy to study the complex. It was concluded that there was not set orientation or distance between the two proteins that made up the complex. Another study, conducted in 2007, used Fourier transform infrared spectroscopy to study the effects of pH on the reduced-minus-oxidized FTIR spectra. This research study found two pH dependent processes. A third study on Paracoccus denitrificans, published in 2007, studied the mechanism that reduces NO to N2O. The study concluded that the protons used in these reactions are taken up from the periplasm and not due to a proton electrochemical gradient.

References

[Sample reference] Takai, K., Sugai, A., Itoh, T., and Horikoshi, K. "Palaeococcus ferrophilus gen. nov., sp. nov., a barophilic, hyperthermophilic archaeon from a deep-sea hydrothermal vent chimney". International Journal of Systematic and Evolutionary Microbiology. 2000. Volume 50. p. 489-500.

Edited by student of Rachel Larsen and Kit Pogliano